ES2304573T3 - Recipiente para llevar a cabo y controlar procesos biologicos. - Google Patents
Recipiente para llevar a cabo y controlar procesos biologicos. Download PDFInfo
- Publication number
- ES2304573T3 ES2304573T3 ES04022352T ES04022352T ES2304573T3 ES 2304573 T3 ES2304573 T3 ES 2304573T3 ES 04022352 T ES04022352 T ES 04022352T ES 04022352 T ES04022352 T ES 04022352T ES 2304573 T3 ES2304573 T3 ES 2304573T3
- Authority
- ES
- Spain
- Prior art keywords
- sample
- temperature
- container
- fluorescence
- samples
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title description 75
- 230000008569 process Effects 0.000 title description 15
- 239000000523 sample Substances 0.000 claims abstract description 424
- 230000003321 amplification Effects 0.000 claims abstract description 86
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 86
- 239000004033 plastic Substances 0.000 claims abstract description 54
- 229920003023 plastic Polymers 0.000 claims abstract description 54
- 239000011521 glass Substances 0.000 claims abstract description 46
- 239000000463 material Substances 0.000 claims abstract description 40
- 239000012472 biological sample Substances 0.000 claims abstract description 23
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 13
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 10
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 10
- 239000002131 composite material Substances 0.000 claims abstract description 3
- 238000006243 chemical reaction Methods 0.000 claims description 71
- 239000012530 fluid Substances 0.000 claims description 59
- 238000001514 detection method Methods 0.000 claims description 52
- 230000005284 excitation Effects 0.000 claims description 28
- 239000000203 mixture Substances 0.000 claims description 14
- 238000003753 real-time PCR Methods 0.000 claims description 2
- 238000007789 sealing Methods 0.000 abstract description 4
- 239000000047 product Substances 0.000 description 91
- 238000010438 heat treatment Methods 0.000 description 47
- 238000004925 denaturation Methods 0.000 description 45
- 230000036425 denaturation Effects 0.000 description 45
- 108020004414 DNA Proteins 0.000 description 42
- 230000003287 optical effect Effects 0.000 description 28
- 238000012546 transfer Methods 0.000 description 28
- 230000004544 DNA amplification Effects 0.000 description 22
- 239000000975 dye Substances 0.000 description 22
- 239000013615 primer Substances 0.000 description 22
- 238000007664 blowing Methods 0.000 description 21
- 238000001816 cooling Methods 0.000 description 21
- 238000003752 polymerase chain reaction Methods 0.000 description 21
- 238000009396 hybridization Methods 0.000 description 20
- 230000000694 effects Effects 0.000 description 19
- 230000004044 response Effects 0.000 description 18
- 230000008901 benefit Effects 0.000 description 17
- 239000007788 liquid Substances 0.000 description 17
- 230000004888 barrier function Effects 0.000 description 16
- 239000003153 chemical reaction reagent Substances 0.000 description 15
- 230000007423 decrease Effects 0.000 description 15
- 230000005855 radiation Effects 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 14
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 13
- 238000002844 melting Methods 0.000 description 13
- 230000008018 melting Effects 0.000 description 13
- 239000003921 oil Substances 0.000 description 13
- 238000005516 engineering process Methods 0.000 description 12
- 238000001917 fluorescence detection Methods 0.000 description 12
- 239000011159 matrix material Substances 0.000 description 12
- 230000007704 transition Effects 0.000 description 12
- 241000282414 Homo sapiens Species 0.000 description 11
- 238000010586 diagram Methods 0.000 description 10
- 230000006870 function Effects 0.000 description 10
- 238000012544 monitoring process Methods 0.000 description 10
- 238000011002 quantification Methods 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 9
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 9
- 102000053602 DNA Human genes 0.000 description 8
- 230000008859 change Effects 0.000 description 8
- 238000004891 communication Methods 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 8
- 230000004927 fusion Effects 0.000 description 8
- 230000008878 coupling Effects 0.000 description 7
- 238000010168 coupling process Methods 0.000 description 7
- 238000005859 coupling reaction Methods 0.000 description 7
- 239000012634 fragment Substances 0.000 description 7
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 6
- 230000009471 action Effects 0.000 description 6
- 230000031018 biological processes and functions Effects 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 239000011541 reaction mixture Substances 0.000 description 6
- 230000000717 retained effect Effects 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 5
- 108091034117 Oligonucleotide Proteins 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 125000004122 cyclic group Chemical group 0.000 description 5
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 5
- 229960005542 ethidium bromide Drugs 0.000 description 5
- 230000007062 hydrolysis Effects 0.000 description 5
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- 230000000670 limiting effect Effects 0.000 description 5
- 239000004417 polycarbonate Substances 0.000 description 5
- 108060002716 Exonuclease Proteins 0.000 description 4
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 4
- 108010010677 Phosphodiesterase I Proteins 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 102000013165 exonuclease Human genes 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 239000011810 insulating material Substances 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 238000011068 loading method Methods 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 230000001869 rapid Effects 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 108010085238 Actins Proteins 0.000 description 3
- 102000007469 Actins Human genes 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 101000899111 Homo sapiens Hemoglobin subunit beta Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 230000001154 acute effect Effects 0.000 description 3
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- 230000008020 evaporation Effects 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 238000005194 fractionation Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 238000012207 quantitative assay Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 229910052724 xenon Inorganic materials 0.000 description 3
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000005355 Hall effect Effects 0.000 description 2
- 241000711549 Hepacivirus C Species 0.000 description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 239000013060 biological fluid Substances 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000009833 condensation Methods 0.000 description 2
- 230000005494 condensation Effects 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000003708 edge detection Methods 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 230000002964 excitative effect Effects 0.000 description 2
- 238000001506 fluorescence spectroscopy Methods 0.000 description 2
- 229910052736 halogen Inorganic materials 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- 208000002672 hepatitis B Diseases 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000012212 insulator Substances 0.000 description 2
- 239000000138 intercalating agent Substances 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 239000003068 molecular probe Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 239000002751 oligonucleotide probe Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 150000008300 phosphoramidites Chemical class 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 239000012780 transparent material Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 229910001369 Brass Inorganic materials 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 229910001006 Constantan Inorganic materials 0.000 description 1
- 201000003883 Cystic fibrosis Diseases 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 229920004943 Delrin® Polymers 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- QTANTQQOYSUMLC-UHFFFAOYSA-O Ethidium cation Chemical compound C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 QTANTQQOYSUMLC-UHFFFAOYSA-O 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 108091036333 Rapid DNA Proteins 0.000 description 1
- 241000555745 Sciuridae Species 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 241000589596 Thermus Species 0.000 description 1
- ULHRKLSNHXXJLO-UHFFFAOYSA-L Yo-Pro-1 Chemical compound [I-].[I-].C1=CC=C2C(C=C3N(C4=CC=CC=C4O3)C)=CC=[N+](CCC[N+](C)(C)C)C2=C1 ULHRKLSNHXXJLO-UHFFFAOYSA-L 0.000 description 1
- XUQUNBOKLNVMMK-UHFFFAOYSA-N [5-[6-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyhexylcarbamoyl]-6'-(2,2-dimethylpropanoyloxy)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] 2,2-dimethylpropanoate Chemical compound C12=CC=C(OC(=O)C(C)(C)C)C=C2OC2=CC(OC(=O)C(C)(C)C)=CC=C2C21OC(=O)C1=CC(C(=O)NCCCCCCOP(N(C(C)C)C(C)C)OCCC#N)=CC=C21 XUQUNBOKLNVMMK-UHFFFAOYSA-N 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 239000010951 brass Substances 0.000 description 1
- 239000001273 butane Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000013481 data capture Methods 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 230000005279 excitation period Effects 0.000 description 1
- 239000011152 fibreglass Substances 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 239000000976 ink Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 239000005367 kimax Substances 0.000 description 1
- 239000005351 kimble Substances 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 1
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 229910001120 nichrome Inorganic materials 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002987 primer (paints) Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229910052761 rare earth metal Inorganic materials 0.000 description 1
- 150000002910 rare earth metals Chemical class 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 230000003134 recirculating effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000002165 resonance energy transfer Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
- B01L2300/0838—Capillaries
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1838—Means for temperature control using fluid heat transfer medium
- B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/07—Centrifugal type cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Pathology (AREA)
- Clinical Laboratory Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Hematology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Optical Measuring Cells (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Selective Calling Equipment (AREA)
- Microscoopes, Condenser (AREA)
- Control Of Temperature (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US65899396A | 1996-06-04 | 1996-06-04 | |
| US658993 | 1996-06-04 | ||
| EP97929833A EP0906449B1 (en) | 1996-06-04 | 1997-06-04 | System and method for carrying out and monitoring polymerase chain reactions |
| EP03025810A EP1442794B1 (en) | 1996-06-04 | 1997-06-04 | System and method for carrying out and monitoring biological processes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2304573T3 true ES2304573T3 (es) | 2008-10-16 |
Family
ID=24643601
Family Applications (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES04022352T Expired - Lifetime ES2304573T3 (es) | 1996-06-04 | 1997-06-04 | Recipiente para llevar a cabo y controlar procesos biologicos. |
| ES97926890T Expired - Lifetime ES2253778T3 (es) | 1996-06-04 | 1997-06-04 | Sistema y metodos de monitorizacion de una amplificacion de adn mediante fluorescencia. |
| ES06004925T Expired - Lifetime ES2354902T3 (es) | 1996-06-04 | 1997-06-04 | Aparato para llevar a cabo la pcr y monitorización de la reacción en tiempo real durante ciclos de temperatura. |
| ES97929833T Expired - Lifetime ES2215230T3 (es) | 1996-06-04 | 1997-06-04 | Sistema y metodo para llevar a cabo y supervisar reacciones de cadena de polimerasa. |
| ES10174616T Expired - Lifetime ES2434258T3 (es) | 1996-06-04 | 1997-06-04 | Aparato para llevar a cabo la PCR y monitorización de la reacción en tiempo real durante ciclos de temperatura |
| ES03025810T Expired - Lifetime ES2260562T3 (es) | 1996-06-04 | 1997-06-04 | Sistema y metodo para llevar a cabo y supervisar procesos biologicos. |
Family Applications After (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES97926890T Expired - Lifetime ES2253778T3 (es) | 1996-06-04 | 1997-06-04 | Sistema y metodos de monitorizacion de una amplificacion de adn mediante fluorescencia. |
| ES06004925T Expired - Lifetime ES2354902T3 (es) | 1996-06-04 | 1997-06-04 | Aparato para llevar a cabo la pcr y monitorización de la reacción en tiempo real durante ciclos de temperatura. |
| ES97929833T Expired - Lifetime ES2215230T3 (es) | 1996-06-04 | 1997-06-04 | Sistema y metodo para llevar a cabo y supervisar reacciones de cadena de polimerasa. |
| ES10174616T Expired - Lifetime ES2434258T3 (es) | 1996-06-04 | 1997-06-04 | Aparato para llevar a cabo la PCR y monitorización de la reacción en tiempo real durante ciclos de temperatura |
| ES03025810T Expired - Lifetime ES2260562T3 (es) | 1996-06-04 | 1997-06-04 | Sistema y metodo para llevar a cabo y supervisar procesos biologicos. |
Country Status (11)
| Country | Link |
|---|---|
| EP (7) | EP1442794B1 (enExample) |
| JP (2) | JP4278712B2 (enExample) |
| AT (5) | ATE390491T1 (enExample) |
| AU (2) | AU727296B2 (enExample) |
| CA (2) | CA2256612C (enExample) |
| DE (5) | DE69727932T2 (enExample) |
| DK (1) | DK2298931T3 (enExample) |
| ES (6) | ES2304573T3 (enExample) |
| NZ (2) | NZ333137A (enExample) |
| PT (1) | PT2298931E (enExample) |
| WO (2) | WO1997046707A2 (enExample) |
Families Citing this family (243)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2505331T3 (es) * | 1997-02-28 | 2014-10-09 | Cepheid | Montaje para reacción química con intercambio de calor e interrogación óptica |
| DE19811730A1 (de) * | 1998-03-18 | 1999-09-23 | November Ag Molekulare Medizin | Verfahren und Vorrichtung zum Identifizieren einer Markierung |
| EP2316571A3 (en) | 1998-05-01 | 2011-07-27 | Gen-Probe Incorporated | Automated diagnostic analyzer and method |
| US7498164B2 (en) | 1998-05-16 | 2009-03-03 | Applied Biosystems, Llc | Instrument for monitoring nucleic acid sequence amplification reaction |
| US6818437B1 (en) | 1998-05-16 | 2004-11-16 | Applera Corporation | Instrument for monitoring polymerase chain reaction of DNA |
| JP2003524754A (ja) * | 1998-05-16 | 2003-08-19 | ピーイー コーポレイション （エヌワイ） | Ｄｎａのポリメラーゼ連鎖反応をモニタする装置 |
| US6140054A (en) * | 1998-09-30 | 2000-10-31 | University Of Utah Research Foundation | Multiplex genotyping using fluorescent hybridization probes |
| DE19850593A1 (de) * | 1998-11-03 | 2000-05-04 | Biochip Technologies Gmbh | Verfahren zum Nachweis von Hybridisierungsereignissen bei DNA |
| US6303305B1 (en) | 1999-03-30 | 2001-10-16 | Roche Diagnostics, Gmbh | Method for quantification of an analyte |
| US6387621B1 (en) | 1999-04-27 | 2002-05-14 | University Of Utah Research Foundation | Automated analysis of real-time nucleic acid amplification |
| US7410793B2 (en) | 1999-05-17 | 2008-08-12 | Applera Corporation | Optical instrument including excitation source |
| US7423750B2 (en) | 2001-11-29 | 2008-09-09 | Applera Corporation | Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion |
| US20050279949A1 (en) | 1999-05-17 | 2005-12-22 | Applera Corporation | Temperature control for light-emitting diode stabilization |
| US7387891B2 (en) | 1999-05-17 | 2008-06-17 | Applera Corporation | Optical instrument including excitation source |
| WO2001013096A1 (de) * | 1999-08-13 | 2001-02-22 | Zeptosens Ag | Vorrichtung und verfahren zur multianalytbestimmung |
| DE19946296A1 (de) * | 1999-09-28 | 2001-03-29 | Roche Diagnostics Gmbh | Multiplex-PCR zum Nachweis von EHEC-Infektionen |
| WO2001035079A1 (en) * | 1999-11-12 | 2001-05-17 | E. I. Du Pont De Nemours And Company | Fluorometer with low heat-generating light source |
| US6691041B2 (en) | 2000-03-31 | 2004-02-10 | Roche Molecular Systems, Inc. | Method for the efficiency-corrected real-time quantification of nucleic acids |
| DE10045521A1 (de) * | 2000-03-31 | 2001-10-04 | Roche Diagnostics Gmbh | Nukleinsäureamplifikationen |
| US6783934B1 (en) * | 2000-05-01 | 2004-08-31 | Cepheid, Inc. | Methods for quantitative analysis of nucleic acid amplification reaction |
| US6720187B2 (en) | 2000-06-28 | 2004-04-13 | 3M Innovative Properties Company | Multi-format sample processing devices |
| EP2316569A1 (en) * | 2000-06-28 | 2011-05-04 | 3M Innovative Properties Company | Enhanced sample processing devices, systems and methods |
| US6734401B2 (en) | 2000-06-28 | 2004-05-11 | 3M Innovative Properties Company | Enhanced sample processing devices, systems and methods |
| US6563581B1 (en) | 2000-07-14 | 2003-05-13 | Applera Corporation | Scanning system and method for scanning a plurality of samples |
| EP1360324A2 (en) | 2000-08-31 | 2003-11-12 | Mayo Foundation For Medical Education And Research | Detection of varicella-zoster virus |
| DE10044373A1 (de) | 2000-09-08 | 2002-03-21 | Roche Diagnostics Gmbh | Neues Reagenz zur Markierung von Nukleinsäuren |
| WO2002061390A2 (en) | 2001-01-31 | 2002-08-08 | Mayo Foundation For Medical Education And Research | Detection of herpes simplex virus |
| WO2002089844A1 (en) | 2001-05-07 | 2002-11-14 | Mayo Foundation For Medical Education And Research | Legionella detection with fluorescent resonance energy transfer |
| US7226732B2 (en) | 2001-07-16 | 2007-06-05 | Cepheid | Methods, apparatus, and computer programs for verifying the integrity of a probe |
| US7635588B2 (en) | 2001-11-29 | 2009-12-22 | Applied Biosystems, Llc | Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength |
| US6889468B2 (en) | 2001-12-28 | 2005-05-10 | 3M Innovative Properties Company | Modular systems and methods for using sample processing devices |
| US7373253B2 (en) | 2002-02-12 | 2008-05-13 | Idaho Technology | Multi-test analysis of real-time nucleic acid amplification |
| US6593093B1 (en) | 2002-02-20 | 2003-07-15 | Mayo Foundation For Medical Education And Research | Detection of group a Streptococcus |
| US7785776B2 (en) | 2002-05-13 | 2010-08-31 | Idaho Technology, Inc. | Genotyping by amplicon melting curve analysis |
| ES2543587T3 (es) | 2002-05-17 | 2015-08-20 | Life Technologies Corporation | Instrumento óptico que incluye una fuente de excitación |
| AUPS267802A0 (en) | 2002-05-30 | 2002-06-20 | Bio-Molecular Holdings Pty Limited | Improved dna amplification apparatus and method |
| CA2433473A1 (en) * | 2002-07-23 | 2004-01-23 | F. Hoffmann-La Roche Ag | Fluorescent hybridization probes with reduced background |
| US7074598B2 (en) | 2002-09-25 | 2006-07-11 | Mayo Foundation For Medical Education And Research | Detection of vancomycin-resistant enterococcus spp. |
| US7365176B2 (en) | 2002-09-26 | 2008-04-29 | Mayo Foundation For Medical Education And Research | Detection of Epstein-Barr virus |
| US7074599B2 (en) | 2002-09-27 | 2006-07-11 | Mayo Foundation For Medical Education And Research | Detection of mecA-containing Staphylococcus spp. |
| US9850381B2 (en) * | 2002-10-23 | 2017-12-26 | University Of Utah Research Foundation | Amplicon melting analysis with saturation dyes |
| US7582429B2 (en) | 2002-10-23 | 2009-09-01 | University Of Utah Research Foundation | Amplicon melting analysis with saturation dyes |
| EP1564300A4 (en) * | 2002-11-13 | 2007-09-05 | Hitachi High Tech Corp | PROCESS FOR NUCLEIC ACID AMPLIFICATION AND DEVICE THEREFOR |
| US7718361B2 (en) | 2002-12-06 | 2010-05-18 | Roche Molecular Systems, Inc. | Quantitative test for bacterial pathogens |
| CN105349657A (zh) | 2002-12-06 | 2016-02-24 | 霍夫曼-拉罗奇有限公司 | 病原生物的多种分析检测 |
| GB2399776A (en) * | 2003-03-24 | 2004-09-29 | Pa Knowledge Ltd | Cyclical heating and cooling device and associated methods |
| EP2284278A1 (en) | 2003-04-04 | 2011-02-16 | Roche Diagnostics GmbH | Improved system for multi color real time PCR |
| US7148043B2 (en) | 2003-05-08 | 2006-12-12 | Bio-Rad Laboratories, Inc. | Systems and methods for fluorescence detection with a movable detection module |
| US7788039B2 (en) | 2003-09-25 | 2010-08-31 | Roche Molecular Systems, Inc. | Quantitation of nucleic acids using growth curves |
| CA2494571C (en) | 2003-12-02 | 2010-02-09 | F.Hoffmann-La Roche Ag | Oligonucleotides containing molecular rods |
| CA2548205C (en) | 2003-12-03 | 2017-06-27 | Abbott Laboratories | Double stranded linear nucleic acid probe and uses thereof |
| AU2005220746A1 (en) | 2004-03-05 | 2005-09-22 | Medical College Of Ohio | Methods and compositions for assessing nucleic acids and alleles |
| JP2005295877A (ja) * | 2004-04-09 | 2005-10-27 | Taiyo Yuden Co Ltd | 核酸分析方法、分析装置及び分析用ディスク |
| EP1745157A4 (en) | 2004-04-12 | 2008-06-11 | Ohio Med College | METHOD AND COMPOSITIONS FOR TESTING ANALYTES |
| US9657347B2 (en) | 2004-04-20 | 2017-05-23 | University of Utah Research Foundation and BioFire Defense, LLC | Nucleic acid melting analysis with saturation dyes |
| EP2708595A1 (en) | 2004-08-05 | 2014-03-19 | Universal Bio Research Co., Ltd. | Reaction vessel, reaction vessel liquid introducing device, liquid introducing and reaction measuring device, and liquid introducing device |
| EP1627921B1 (de) * | 2004-08-18 | 2008-08-27 | Roche Diagnostics GmbH | Verfahren zur Messung einer Nukleinsäureamplifikation in Real Time beinhaltend die Positionierung eines Reaktionsgefässes relativ zu einer Detektionseinheit |
| GB0419325D0 (en) * | 2004-09-01 | 2004-09-29 | Perkinelmer Ltd | A method of analysing a sample including fluorescent labels and apparatus therefor |
| EP1634965B1 (en) | 2004-09-09 | 2010-01-20 | Roche Diagnostics GmbH | Real time PCR with the addition of pyrophosphatase |
| DE102004043909A1 (de) * | 2004-09-10 | 2006-03-30 | Kendro Laboratory Products Gmbh | Erwärmungsvorrichtung für Proben auf dem Gebiet der Life-Science |
| EP2615160A1 (en) | 2004-10-06 | 2013-07-17 | Universal Bio Research Co., Ltd. | Reaction vessel and reaction controller |
| US20060094028A1 (en) * | 2004-11-04 | 2006-05-04 | Welch Allyn, Inc. | Rapid diagnostic assay |
| DE602005000877T2 (de) | 2005-01-18 | 2007-12-20 | Roche Diagnostics Gmbh | Fluoreszenzabbildung mittels Telezentrizität |
| EP1681555B1 (en) | 2005-01-18 | 2007-03-21 | Roche Diagnostics GmbH | Imaging of fluorescence signals using telecentric excitation and imaging optics |
| US7932081B2 (en) | 2005-03-10 | 2011-04-26 | Gen-Probe Incorporated | Signal measuring system for conducting real-time amplification assays |
| EP2348320B1 (en) * | 2005-03-10 | 2024-05-01 | Gen-Probe Incorporated | Methods and systems for detecting multiple fluorescent emission signals |
| EP1700922B1 (en) | 2005-03-10 | 2016-08-24 | Roche Diagnostics GmbH | 3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them |
| US7759469B2 (en) | 2005-03-10 | 2010-07-20 | Roche Diagnostics Operations, Inc. | Labeling reagent |
| US7680604B2 (en) | 2005-03-11 | 2010-03-16 | Roche Molecular Systems, Inc. | PCR elbow determination by rotational transform after zero slope alignment |
| US7323660B2 (en) | 2005-07-05 | 2008-01-29 | 3M Innovative Properties Company | Modular sample processing apparatus kits and modules |
| US7763210B2 (en) | 2005-07-05 | 2010-07-27 | 3M Innovative Properties Company | Compliant microfluidic sample processing disks |
| US7754474B2 (en) | 2005-07-05 | 2010-07-13 | 3M Innovative Properties Company | Sample processing device compression systems and methods |
| US9017964B2 (en) | 2005-08-26 | 2015-04-28 | Denator Aktiebolag | Preparing biological samples for analysis |
| US20070048877A1 (en) * | 2005-08-26 | 2007-03-01 | Karl Skold | Method and device for preparing a biological sample for biological analyses |
| US7569367B2 (en) | 2005-09-07 | 2009-08-04 | Roche Diagnostics Operations, Inc. | Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy |
| US7991561B2 (en) | 2005-09-29 | 2011-08-02 | Roche Molecular Systems, Inc. | Ct determination by cluster analysis with variable cluster endpoint |
| US7991558B2 (en) | 2005-09-29 | 2011-08-02 | Roche Molecular Systems, Inc. | Systems and methods for determining real-time PCR cycle thresholds using cluster analysis |
| US7781165B2 (en) | 2005-10-19 | 2010-08-24 | Roche Diagnostics Operations, Inc. | Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification |
| US7875425B2 (en) * | 2005-11-09 | 2011-01-25 | Applied Biosystems, Llc | Methods for monitoring polymerase chain reactions |
| GB0522972D0 (en) * | 2005-11-11 | 2005-12-21 | Integrated Design Ltd | Thermal cycler |
| ES2332139T3 (es) | 2005-11-23 | 2010-01-27 | F. Hoffmann-La Roche Ag | Polinucleotidos con mimetico de fosfato. |
| US7680868B2 (en) | 2005-12-20 | 2010-03-16 | Roche Molecular Systems, Inc. | PCR elbow determination by use of a double sigmoid function curve fit with the Levenburg-Marquardt algorithm and normalization |
| US7844403B2 (en) * | 2005-12-20 | 2010-11-30 | Roche Molecular Systems, Inc. | Temperature step correction with double sigmoid Levenberg-Marquardt and robust linear regression |
| US7668663B2 (en) | 2005-12-20 | 2010-02-23 | Roche Molecular Systems, Inc. | Levenberg-Marquardt outlier spike removal method |
| US7991562B2 (en) | 2005-12-20 | 2011-08-02 | Roche Molecular Systems, Inc. | PCR elbow determination using curvature analysis of a double sigmoid |
| US7981606B2 (en) | 2005-12-21 | 2011-07-19 | Roche Molecular Systems, Inc. | Control for nucleic acid testing |
| CA2638854C (en) | 2006-02-27 | 2012-05-15 | F. Hoffmann-La Roche Ag | Pcr hot start by magnesium sequestration |
| US8293473B2 (en) | 2006-04-04 | 2012-10-23 | Labonnet Ltd. | Assessment of reaction kinetics compatibility between polymerase chain reactions |
| US8232091B2 (en) | 2006-05-17 | 2012-07-31 | California Institute Of Technology | Thermal cycling system |
| US8119352B2 (en) | 2006-06-20 | 2012-02-21 | Cepheld | Multi-stage amplification reactions by control of sequence replication times |
| US8097419B2 (en) | 2006-09-12 | 2012-01-17 | Longhorn Vaccines & Diagnostics Llc | Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009 |
| US8080645B2 (en) | 2007-10-01 | 2011-12-20 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection/transport compositions and methods |
| US9481912B2 (en) | 2006-09-12 | 2016-11-01 | Longhorn Vaccines And Diagnostics, Llc | Compositions and methods for detecting and identifying nucleic acid sequences in biological samples |
| US8535888B2 (en) | 2006-12-29 | 2013-09-17 | Mayo Foundation For Medical Education And Research | Compositions and methods for detecting methicillin-resistant S. aureus |
| EP1956097A1 (en) | 2007-02-06 | 2008-08-13 | bioMerieux B.V. | Method for discriminating single nucleotide polymorphisms (SNPs) |
| JP5381100B2 (ja) * | 2007-02-22 | 2014-01-08 | 東洋紡株式会社 | 核酸増幅装置 |
| US9475051B2 (en) | 2007-02-27 | 2016-10-25 | Sony Corporation | Nucleic acid amplifier |
| JP4458133B2 (ja) * | 2007-02-27 | 2010-04-28 | ソニー株式会社 | 核酸増幅装置 |
| JP5191041B2 (ja) | 2007-04-05 | 2013-04-24 | エフ．ホフマン−ラ ロシュ アーゲー | 急速ワンステップｒｔ−ｐｃｒ |
| JPWO2008126827A1 (ja) * | 2007-04-06 | 2010-07-22 | ユニバーサル・バイオ・リサーチ株式会社 | 温度制御装置および温度制御方法 |
| JP5444632B2 (ja) * | 2007-04-26 | 2014-03-19 | 東洋紡株式会社 | 核酸増幅方法およびそれに用いる容器 |
| EP2182049A1 (en) | 2007-05-23 | 2010-05-05 | Trust Medical Co., Ltd. | Container for liquid reaction mixture, reaction-promoting device using the same and method therefor |
| US11041215B2 (en) | 2007-08-24 | 2021-06-22 | Longhorn Vaccines And Diagnostics, Llc | PCR ready compositions and methods for detecting and identifying nucleic acid sequences |
| US8386184B2 (en) | 2007-08-28 | 2013-02-26 | Becton, Dickinson And Company | Systems and methods for determining an amount of starting reagent using the polymerase chain reaction |
| DE102007041864B4 (de) | 2007-09-04 | 2012-05-03 | Sirs-Lab Gmbh | Verfahren zum Nachweis von Bakterien und Pilzen |
| US20090119020A1 (en) | 2007-09-25 | 2009-05-07 | Roche Molecular Systems, Inc. | Pcr elbow determination using quadratic test for curvature analysis of a double sigmoid |
| BRPI0816047A2 (pt) | 2007-09-28 | 2018-06-05 | 3M Innovative Properties Co | conjunto de oligonucleotídeos para detecção de um ácido nucleico alvo, métodos para detecção da presença ou ausência de um ácido nucleico alvo e de pelo menos dois ácidos nucleicos alvo, kit, mistura de reação e amplicon identificado por fluorescência |
| MX352987B (es) | 2007-10-02 | 2017-12-15 | Theranos Ip Co Llc | Dispositivos modulares de punto de cuidado y usos de los mismos. |
| JP5178149B2 (ja) * | 2007-10-31 | 2013-04-10 | キヤノン株式会社 | 標的核酸のリアルタイムｐｃｒ方法及びその装置 |
| DE102007063102B4 (de) | 2007-12-28 | 2022-02-10 | Robert Bosch Gmbh | Verfahren zur Erfassung eines periodisch pulsierenden Betriebsparameters |
| EP2238459B1 (en) * | 2008-01-23 | 2019-05-08 | Roche Diagnostics GmbH | Integrated instrument performing synthesis and amplification |
| JP2011510675A (ja) * | 2008-02-07 | 2011-04-07 | フォレンシック サイエンシーズ サーヴィス リミテッド | 分析の及び分析に関する改善 |
| JP5665548B2 (ja) | 2008-02-08 | 2015-02-04 | メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ | クロストリジウム・ディフィシレの検出 |
| DE102008010436A1 (de) * | 2008-02-21 | 2009-09-03 | Berthold Technologies Gmbh & Co. Kg | Vorrichtung und Verfahren zur Messung von Lumineszenz und Fluoreszenz von transfizierten Zellen oder Organteilen |
| JP2009201444A (ja) * | 2008-02-29 | 2009-09-10 | Hitachi High-Technologies Corp | 核酸分析装置 |
| CA2658520C (en) | 2008-03-19 | 2016-11-08 | F. Hoffmann-La Roche Ag | Nucleic acid amplification in the presence of modified randomers |
| GB0806676D0 (en) | 2008-04-12 | 2008-05-14 | Environment Agency The | Environmental monitoring |
| ES2439951T3 (es) | 2008-06-06 | 2014-01-27 | F. Hoffmann-La Roche Ag | Detección y cuantificación multiplex de ácidos nucleicos microbianos controlada de forma interna |
| EP2138588A1 (en) | 2008-06-23 | 2009-12-30 | Koninklijke Philips Electronics N.V. | Melting curve measurement during amplification |
| US8097412B2 (en) | 2008-07-12 | 2012-01-17 | Biodiagnostics, Inc. | DNA-based test for detection of annual and intermediate ryegrass |
| EP2148187A1 (de) | 2008-07-25 | 2010-01-27 | Roche Diagnostics GmbH | Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion |
| CA2638458A1 (en) | 2008-07-31 | 2010-01-31 | Spartan Bioscience Inc. | Thermal recycling by positioning relative to fixed-temperature source |
| CN102112631A (zh) | 2008-08-01 | 2011-06-29 | 霍夫曼-拉罗奇有限公司 | 用于mRNA扩增的改进的裂解和逆转录 |
| US7910720B2 (en) | 2008-09-09 | 2011-03-22 | Roche Diagnostics Operations, Inc. | Polyanion for improved nucleic acid amplification |
| US8219324B2 (en) | 2008-09-12 | 2012-07-10 | Roche Molecular Systems, Inc. | Real-time PCR elbow calling by equation-less algorithm |
| CA2682439A1 (en) | 2008-10-17 | 2010-04-17 | F. Hoffmann-La Roche Ag | Cell monitoring and molecular analysis |
| US8346485B2 (en) | 2008-11-25 | 2013-01-01 | Quest Diagnostics Investments Incorporated | Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction |
| CA2688174C (en) | 2008-12-19 | 2018-08-07 | F. Hoffmann-La Roche Ag | Dry composition of reaction compounds with stabilized polymerase |
| KR101423936B1 (ko) * | 2009-03-11 | 2014-07-29 | (주)바이오니아 | 실시간 핵산 분석 통합 장치 및 이를 이용한 타겟 핵산의 검출방법 |
| CN105154326A (zh) * | 2009-03-31 | 2015-12-16 | 财团法人神奈川科学技术研究院 | 液体回流型高速基因扩增装置 |
| JP2012530243A (ja) | 2009-06-15 | 2012-11-29 | ビージー リサーチ エルティーディー | 核酸検出方法 |
| US8219366B2 (en) | 2009-08-26 | 2012-07-10 | Roche Molecular Sytems, Inc. | Determination of elbow values for PCR for parabolic shaped curves |
| US10288632B2 (en) * | 2009-09-21 | 2019-05-14 | Pocared Diagnostics Ltd. | System for conducting the identification of bacteria in biological samples |
| FI20096021A0 (fi) | 2009-10-06 | 2009-10-06 | Wallac Oy | Optinen mittausinstrumentti |
| US8834792B2 (en) | 2009-11-13 | 2014-09-16 | 3M Innovative Properties Company | Systems for processing sample processing devices |
| US20110250598A1 (en) | 2010-04-12 | 2011-10-13 | Ulrike Fischer | Detergent free polymerases |
| EP2743705B1 (en) | 2010-07-23 | 2016-10-12 | Beckman Coulter, Inc. | System or method of including analytical units |
| DE112011102524T5 (de) | 2010-07-29 | 2013-07-11 | Roche Diagnostics Gmbh | Präparation generischer Proben |
| US9046507B2 (en) | 2010-07-29 | 2015-06-02 | Gen-Probe Incorporated | Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure |
| WO2012013734A1 (en) | 2010-07-29 | 2012-02-02 | F. Hoffmann - La Roche Ag | Control nucleic acids for multiple parameters |
| CA2802548C (en) | 2010-07-29 | 2017-01-03 | F. Hoffmann-La Roche Ag | Qualitative and quantitative detection of microbial nucleic acids |
| WO2012013732A1 (en) | 2010-07-29 | 2012-02-02 | F. Hoffmann - La Roche Ag | Generic pcr |
| EP2625285B1 (en) | 2010-10-04 | 2020-04-29 | Roche Diagniostics GmbH | Method for cell lysis and pcr within the same reaction vessel |
| WO2012045668A1 (en) | 2010-10-04 | 2012-04-12 | Roche Diagnostics Gmbh | Method for cell lysis in a rt-pcr reaction buffer |
| EP2625286B1 (en) | 2010-10-04 | 2020-04-15 | Roche Diagniostics GmbH | Method for cell lysis in a pcr reaction buffer |
| US9057101B2 (en) | 2010-10-15 | 2015-06-16 | Universal Bio Research Co., Ltd. | Automated nucleic acid processor and automated nucleic acid processing method using multi function dispensing unit |
| US8361720B2 (en) | 2010-11-15 | 2013-01-29 | Exact Sciences Corporation | Real time cleavage assay |
| EP2465945A1 (en) | 2010-12-17 | 2012-06-20 | F. Hoffmann-La Roche AG | Generic matrix for control nucleic acids |
| CN106290159A (zh) | 2011-01-21 | 2017-01-04 | 提拉诺斯公司 | 样品使用最大化的系统和方法 |
| USD684273S1 (en) | 2011-02-22 | 2013-06-11 | Universal Bio Research Co., Ltd. | Reaction vessel for amplifying nucleic acid |
| US8718948B2 (en) | 2011-02-24 | 2014-05-06 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
| ES2744237T3 (es) | 2011-05-18 | 2020-02-24 | Diasorin S P A | Sistemas y métodos de distribución en un dispositivo de procesamiento de muestra |
| AU2012255151B2 (en) | 2011-05-18 | 2015-09-03 | Diasorin Italia S.P.A. | Systems and methods for detecting the presence of a selected volume of material in a sample processing device |
| MX336625B (es) | 2011-05-18 | 2016-01-26 | 3M Innovative Properties Co | Sistemas y metodos para medicion volumetrica en dispositivo de procesamiento de muestra. |
| US9034581B2 (en) | 2011-05-26 | 2015-05-19 | Roche Molecular Systems, Inc. | Compositions and methods for detection of Staphylococcus aureus |
| US9632102B2 (en) | 2011-09-25 | 2017-04-25 | Theranos, Inc. | Systems and methods for multi-purpose analysis |
| US9664702B2 (en) | 2011-09-25 | 2017-05-30 | Theranos, Inc. | Fluid handling apparatus and configurations |
| US8475739B2 (en) | 2011-09-25 | 2013-07-02 | Theranos, Inc. | Systems and methods for fluid handling |
| US20140170735A1 (en) | 2011-09-25 | 2014-06-19 | Elizabeth A. Holmes | Systems and methods for multi-analysis |
| US10012664B2 (en) | 2011-09-25 | 2018-07-03 | Theranos Ip Company, Llc | Systems and methods for fluid and component handling |
| US9810704B2 (en) | 2013-02-18 | 2017-11-07 | Theranos, Inc. | Systems and methods for multi-analysis |
| CN104114282B (zh) | 2011-09-30 | 2017-07-04 | 生命技术公司 | 用于生物分析的系统和方法 |
| BR112014011048A2 (pt) | 2011-11-07 | 2017-05-02 | Beckman Coulter Inc | braço robótico |
| EP2776846B1 (en) | 2011-11-07 | 2019-08-21 | Beckman Coulter, Inc. | Aliquotter system and workflow |
| JP6320926B2 (ja) | 2011-11-07 | 2018-05-09 | ベックマン コールター， インコーポレイテッド | 遠心分離機システムおよびワークフロー |
| ES2778054T3 (es) | 2011-11-07 | 2020-08-07 | Beckman Coulter Inc | Sistema y método para transportar recipientes de muestras |
| WO2013070748A1 (en) | 2011-11-07 | 2013-05-16 | Beckman Coulter, Inc. | Magnetic damping for specimen transport system |
| WO2013070744A2 (en) | 2011-11-07 | 2013-05-16 | Beckman Coulter, Inc. | Specimen container detection |
| EP2828400B1 (en) | 2012-03-21 | 2018-09-19 | Roche Diagniostics GmbH | Methods for assessing rna quality |
| US9982313B2 (en) | 2012-08-17 | 2018-05-29 | Roche Molecular Systems, Inc. | Compositions and methods for detection of herpes simplex virus 1 and 2 |
| DE102012108158B4 (de) | 2012-09-03 | 2016-03-17 | Johann Wolfgang Goethe-Universität | Kapillarzelle, Anordnung und Verfahren zur Aufnahme, zur Positionierung und zur Untersuchung einer mikroskopischen Probe |
| US10176293B2 (en) | 2012-10-02 | 2019-01-08 | Roche Molecular Systems, Inc. | Universal method to determine real-time PCR cycle threshold values |
| EP2722399A1 (en) | 2012-10-18 | 2014-04-23 | Roche Diagniostics GmbH | Method for preventing high molecular weight products during amplification |
| EP4480582A1 (en) | 2013-01-18 | 2024-12-25 | Biomeme, Inc. | Analytic device |
| WO2014122924A1 (ja) | 2013-02-05 | 2014-08-14 | パナソニックヘルスケア株式会社 | 解析装置および遺伝子解析方法、解析容器、ファジィ制御の制御方法 |
| AU2013202788B2 (en) * | 2013-03-14 | 2015-10-01 | Gen-Probe Incorporated | Indexing signal detection module |
| JP6307500B2 (ja) | 2013-05-21 | 2018-04-04 | ユニバーサル・バイオ・リサーチ株式会社 | シーケンサ前処理装置およびその方法 |
| US10072288B2 (en) | 2013-11-11 | 2018-09-11 | Roche Molecular Systems, Inc. | Detecting single nucleotide polymorphism using overlapped primer and melting probe |
| US9745618B2 (en) | 2014-11-19 | 2017-08-29 | Roche Molecular Systems, Inc. | Photoblocked probes and methods for sequential detection of nucleic acids |
| US9920381B2 (en) | 2014-12-02 | 2018-03-20 | Roche Molecular Systems, Inc. | Compositions and methods for detecting MECC-containing methicillin-resistant Staphylococcus aureus |
| FR3035411A1 (fr) * | 2015-04-23 | 2016-10-28 | Morpho | Procede d'amplification d'acide nucleique a des fins d'analyse, machine d'amplification correspondante et cartouche pour cette machine |
| US11266730B2 (en) | 2015-09-29 | 2022-03-08 | The General Hospital Corporation | Methods of treating and diagnosing disease using biomarkers for BCG therapy |
| EP3387107B1 (en) | 2015-12-11 | 2020-08-12 | Spartan Bioscience Inc. | Tube sealing system and methods for nucleic acid amplification |
| CA3017265C (en) | 2016-03-11 | 2022-08-16 | F. Hoffmann-La Roche Ag | Compositions and methods for detection of zika virus |
| ES2929367T3 (es) | 2016-05-18 | 2022-11-28 | Hoffmann La Roche | Amplificación por PCR ultrarrápida cuantitativa usando un dispositivo basado en electrohumectación |
| WO2017202894A1 (en) | 2016-05-27 | 2017-11-30 | Roche Diagnostics Gmbh | Compositions and methods for detection of trichomonas vaginalis |
| US10612101B2 (en) | 2016-05-27 | 2020-04-07 | Roche Molecular Systems, Inc. | Compositions and methods for detection of Mycoplasma genitalium |
| US20170356025A1 (en) | 2016-06-14 | 2017-12-14 | Roche Molecular Systems, Inc. | Internal control probes for improving pcr assay performance |
| AU2017301881A1 (en) | 2016-07-29 | 2019-02-07 | Juno Therapeutics, Inc. | Methods for assessing the presence or absence of replication competent virus |
| WO2018024562A1 (en) | 2016-08-02 | 2018-02-08 | Roche Diagnostics Gmbh | Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids |
| CN107964507B (zh) * | 2016-10-18 | 2022-09-27 | 财团法人工业技术研究院 | 热对流聚合酶连锁反应装置及其光学检测方法 |
| US10793923B2 (en) | 2016-11-09 | 2020-10-06 | Roche Molecular Systems, Inc. | Compositions and methods for detection of BK virus |
| US20190211377A1 (en) | 2016-12-22 | 2019-07-11 | Roche Molecular Systems, Inc. | Cobra probes to detect a marker for epidemic ribotypes of clostridium difficile |
| US10793901B2 (en) | 2016-12-28 | 2020-10-06 | Roche Molecular Systems, Inc. | Reversibly protected nucleotide reagents with high thermal stability |
| CN108738349A (zh) | 2016-12-28 | 2018-11-02 | 达纳福股份有限公司 | 分析装置 |
| US20180363044A1 (en) | 2017-06-14 | 2018-12-20 | Roche Molecular Systems, Inc. | Compositions and methods for improving the thermal stability of nucleic acid amplification reagents |
| US10760137B2 (en) | 2017-07-18 | 2020-09-01 | Roche Molecular Systems, Inc. | Compositions and methods for detection of Babesia |
| JP7293236B2 (ja) | 2017-09-15 | 2023-06-19 | バイオミーム インコーポレイテッド | 自動試料処理のための方法およびシステム |
| WO2019063661A1 (en) | 2017-09-29 | 2019-04-04 | Roche Diagnostics Gmbh | COMPOSITIONS AND METHODS FOR THE DETECTION OF TRICHOMONAS VAGINALIS |
| US11078534B2 (en) | 2017-11-27 | 2021-08-03 | Roche Molecular Systems, Inc. | Photocleavable nucleotide reagents with high stability |
| CN111712581A (zh) | 2017-12-15 | 2020-09-25 | 生米公司 | 用于分析样品的便携式装置和方法 |
| US11535903B2 (en) | 2018-01-31 | 2022-12-27 | Juno Therapeutics, Inc. | Methods and reagents for assessing the presence or absence of replication competent virus |
| WO2019155488A1 (en) * | 2018-02-07 | 2019-08-15 | Jaydeep Bhattacharya | A low cost microfluidic device for dna/rna isolation, purification and amplification using chip based pcr/ rt- pcr for biosensing applications |
| US20190292596A1 (en) | 2018-03-21 | 2019-09-26 | Roche Molecular Systems, Inc. | Modified nucleoside phosphates with high thermal stability |
| US11384376B2 (en) | 2018-05-31 | 2022-07-12 | Roche Molecular Systems, Inc. | Reagents and methods for post-synthetic modification of nucleic acids |
| GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
| JPWO2020032226A1 (ja) | 2018-08-10 | 2021-08-10 | 株式会社Ｍｉｒａｉ Ｇｅｎｏｍｉｃｓ | 分析装置 |
| EP3891302B1 (en) | 2018-12-03 | 2025-04-16 | F. Hoffmann-La Roche AG | Compositions and methods for detection of candida auris |
| CN113874708A (zh) | 2019-03-21 | 2021-12-31 | 生米公司 | 多功能分析装置 |
| CN116786186A (zh) * | 2019-05-01 | 2023-09-22 | 卢米耐克斯公司 | 用于热循环的装置以及样品模块 |
| CN113785073A (zh) | 2019-05-02 | 2021-12-10 | 豪夫迈·罗氏有限公司 | 基于链分离温度利用dITP进行RNA靶标相比于DNA靶标的优先性/选择性扩增 |
| JP7592027B2 (ja) | 2019-05-07 | 2024-11-29 | エフ． ホフマン－ラ ロシュ アーゲー | ナイセリア・ゴノレア（Ｎｅｉｓｓｅｒｉａ Ｇｏｎｏｒｒｏｈｅａｅ）を検出するための組成物および方法 |
| EP4004240B1 (en) | 2019-07-25 | 2024-03-13 | F. Hoffmann-La Roche AG | Compositions and methods for detection of epstein barr virus (ebv) |
| ES3039650T3 (en) | 2019-08-27 | 2025-10-23 | Hoffmann La Roche | Compositions and methods for amplification and detection of hepatitis b virus rna, including hbv rna transcribed from cccdna |
| US11441167B2 (en) | 2019-11-20 | 2022-09-13 | Roche Molecular Systems, Inc. | Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi |
| US20230050863A1 (en) | 2019-12-09 | 2023-02-16 | Roche Molecular Systems, Inc. | Dicationic fluorescent dyes |
| JP7716408B2 (ja) | 2019-12-27 | 2025-07-31 | エフ． ホフマン－ラ ロシュ アーゲー | メチシリン耐性黄色ブドウ球菌を検出するための組成物および方法 |
| CN116171333A (zh) | 2020-03-09 | 2023-05-26 | 豪夫迈·罗氏有限公司 | 用于检测严重急性呼吸综合征冠状病毒2(sars-cov-2)、甲型流感和乙型流感的组合物和方法 |
| WO2022061105A1 (en) | 2020-09-18 | 2022-03-24 | Biomeme, Inc. | Portable devices and methods for analyzing samples |
| JP2023552546A (ja) | 2020-12-04 | 2023-12-18 | エフ． ホフマン－ラ ロシュ アーゲー | マラリアを検出するための組成物および方法 |
| JP7209980B2 (ja) | 2020-12-11 | 2023-01-23 | 東洋紡株式会社 | Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体 |
| JP2022099005A (ja) | 2020-12-22 | 2022-07-04 | 船井電機株式会社 | マイクロ流体デバイスおよび核酸増幅方法 |
| US20220205020A1 (en) | 2020-12-30 | 2022-06-30 | Roche Molecular Systems, Inc. | Compositions and methods for detection of bacteria and fungi associated with bacterial and candida vaginosis |
| EP4288567A1 (en) | 2021-02-05 | 2023-12-13 | F. Hoffmann-La Roche AG | Compositions and methods for detection of human parainfluenza viruses 1-4 (hpiv 1-4) |
| US20220290221A1 (en) | 2021-03-15 | 2022-09-15 | Roche Molecular Systems, Inc. | Compositions and methods for detecting severe acute respiratory syndrome coronavirus 2 (sars-cov-2) variants having spike protein mutations |
| JP2024511359A (ja) | 2021-03-19 | 2024-03-13 | ビージー リサーチ エルティーディー | 熱サイクリングのための装置及び関連する方法 |
| CN117858960A (zh) | 2021-05-06 | 2024-04-09 | 豪夫迈·罗氏有限公司 | 用于通过双靶标测定来检测丁型肝炎病毒的组合物和方法 |
| CN113355230B (zh) * | 2021-07-06 | 2023-11-24 | 宁波胤瑞生物医学仪器有限责任公司 | 芯片锁紧装置 |
| TWI876600B (zh) * | 2021-07-08 | 2025-03-11 | 安盛生科股份有限公司 | 進行定量聚合酶鏈反應之系統及方法 |
| KR20240065279A (ko) | 2021-09-23 | 2024-05-14 | 엔6 테크, 인크. | 샘플 분석을 위한 방법 및 시스템 |
| WO2023079032A1 (en) | 2021-11-05 | 2023-05-11 | F. Hoffmann-La Roche Ag | Compositions and methods for detection of malaria |
| US20250011882A1 (en) | 2021-11-22 | 2025-01-09 | Roche Molecular Systems, Inc. | Compositions and Methods for Detecting vanA and/or vanB Genes Associated with Multidrug Resistance |
| CA3241326A1 (en) | 2021-12-03 | 2023-06-08 | Medicinal Genomics Corporation | Psilocybe assay |
| WO2024003260A1 (en) | 2022-06-30 | 2024-01-04 | F. Hoffmann-La Roche Ag | Compositions and methods for detecting lymphogranuloma venereum (lgv) serovars of chlamydia trachomatis |
| WO2024042042A1 (en) | 2022-08-24 | 2024-02-29 | F. Hoffmann-La Roche Ag | Compositions and methods for detecting monkeypox virus |
| EP4587594A1 (en) | 2022-09-13 | 2025-07-23 | Medicinal Genomics Corporation | <smallcapsilocybe assay |
| CN115555060B (zh) * | 2022-09-22 | 2024-11-05 | 冰山松洋生物科技(大连)有限公司 | 一种环境试验箱 |
| CN120418449A (zh) | 2022-12-25 | 2025-08-01 | 豪夫迈·罗氏有限公司 | 用于检测耐碳青霉烯的醋酸钙不动杆菌-鲍氏不动杆菌(crab)的组合物和方法 |
| CN120731278A (zh) | 2023-02-25 | 2025-09-30 | 豪夫迈·罗氏有限公司 | 用于念珠菌属物种检测的组合物和方法 |
| CN120917150A (zh) | 2023-03-03 | 2025-11-07 | 豪夫迈·罗氏有限公司 | 用于检测b族链球菌（无乳链球菌）和克林霉素抗性基因决定子的组合物和方法 |
| WO2024235904A1 (en) | 2023-05-16 | 2024-11-21 | Roche Molecular Systems, Inc. | Detection of target analytes using multimodal signal probes |
| WO2025027080A1 (en) | 2023-08-03 | 2025-02-06 | F. Hoffmann-La Roche Ag | Use of 5'-tailed long primers to improve amplification performance when targeting short primer-binding sites |
| WO2025202488A1 (en) | 2024-03-28 | 2025-10-02 | Roche Diagnostics Gmbh | Enhancer of nucleic acid amplification |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3219416A (en) * | 1962-10-30 | 1965-11-23 | Scientific Industries | Apparatus for the automatic chemical sequential treatment and analysis of small quantities of material |
| US3556659A (en) * | 1966-02-03 | 1971-01-19 | Applied Physics Corp | Laser-excited raman spectrometer |
| US3616264A (en) | 1969-06-30 | 1971-10-26 | Beckman Instruments Inc | Temperature-controlled discrete sample analyzer |
| US3718133A (en) * | 1971-01-12 | 1973-02-27 | Damon Corp | Container unit for liquid samples |
| CH587486A5 (enExample) * | 1974-11-29 | 1977-05-13 | Hoffmann La Roche | |
| AU528259B2 (en) * | 1978-09-14 | 1983-04-21 | Union Carbide Corp | Method and apparatus for assaying liquid materials |
| IT1101295B (it) | 1978-11-22 | 1985-09-28 | Erba Strumentazione | Camera gas-cromatografica |
| US4420679A (en) | 1982-02-26 | 1983-12-13 | Delta Associates, Inc. | Gas chromatographic oven using symmetrical flow of preheated - premixed ambient air |
| JPS60241884A (ja) * | 1984-05-15 | 1985-11-30 | Tokyo Daigaku | 自動サイクリング反応装置およびこれを用いる自動分析装置 |
| US4762413A (en) * | 1984-09-07 | 1988-08-09 | Olympus Optical Co., Ltd. | Method and apparatus for measuring immunological reaction with the aid of fluctuation in intensity of scattered light |
| US4740472A (en) * | 1985-08-05 | 1988-04-26 | The United States Of America As Represented By The United States Department Of Energy | Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer |
| CA1273552A (en) * | 1985-12-23 | 1990-09-04 | Michael J. Heller | Fluorescent stokes shift probes for polynucleotide hybridization assays |
| US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
| US4902624A (en) * | 1987-11-23 | 1990-02-20 | Eastman Kodak Company | Temperature cycling cuvette |
| US4908112A (en) * | 1988-06-16 | 1990-03-13 | E. I. Du Pont De Nemours & Co. | Silicon semiconductor wafer for analyzing micronic biological samples |
| DE69012887D1 (de) * | 1989-06-20 | 1994-11-03 | Claudio Bonini | Prüfröhrchen mit einer linsenförmigen Aussenfläche, insbesondere für automatische klinische Analysen. |
| US5449621A (en) * | 1989-07-31 | 1995-09-12 | Biotope, Inc. | Method for measuring specific binding assays |
| US5455175A (en) * | 1990-06-04 | 1995-10-03 | University Of Utah Research Foundation | Rapid thermal cycling device |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| WO1992020778A1 (en) * | 1991-05-24 | 1992-11-26 | Kindconi Pty Limited | Biochemical reaction control |
| US5340715A (en) * | 1991-06-07 | 1994-08-23 | Ciba Corning Diagnostics Corp. | Multiple surface evanescent wave sensor with a reference |
| DE4234086A1 (de) † | 1992-02-05 | 1993-08-12 | Diagen Inst Molekularbio | Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen |
| DE69207580T2 (de) * | 1992-03-23 | 1996-08-08 | Hoffmann La Roche | Verfahren zum DNS-Nachweis |
| AU4047893A (en) * | 1992-04-06 | 1993-11-08 | Abbott Laboratories | Method and device for detection of nucleic acid or analyte using total internal reflectance |
| JPH0634546A (ja) * | 1992-07-17 | 1994-02-08 | Tosoh Corp | 蛍光検出器 |
| US5798215A (en) * | 1993-02-18 | 1998-08-25 | Biocircuits Corporation | Device for use in analyte detection assays |
| AU6104994A (en) * | 1993-03-18 | 1994-10-11 | Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture | Apparatus for the quantitative determination of particulate analytes |
| JP3426602B2 (ja) * | 1993-05-18 | 2003-07-14 | ユニヴァーシティ オブ ユタ リサーチ ファンデーション | 多分析物均質蛍光免疫検定用の装置および方法 |
| JP3598123B2 (ja) * | 1993-07-15 | 2004-12-08 | 浜松ホトニクス株式会社 | 核酸の変性検出装置 |
| ATE208658T1 (de) * | 1993-07-28 | 2001-11-15 | Pe Corp Ny | Vorrichtung und verfahren zur nukleinsäurevervielfältigung |
| CA2129787A1 (en) * | 1993-08-27 | 1995-02-28 | Russell G. Higuchi | Monitoring multiple amplification reactions simultaneously and analyzing same |
| DK1921169T3 (da) * | 1993-11-12 | 2012-06-04 | Phri Properties Inc | Hybridiseringssonder til nukleinsyredetektion, universelle stamceller, fremgangsmåder og kits |
| US5654419A (en) * | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
| JP3383310B2 (ja) * | 1994-02-01 | 2003-03-04 | ロバート イー． フィールズ， | 分子分析器および使用方法 |
| CA2143365A1 (en) * | 1994-03-14 | 1995-09-15 | Hugh V. Cottingham | Nucleic acid amplification method and apparatus |
| ATE198511T1 (de) * | 1994-04-29 | 2001-01-15 | Perkin Elmer Corp | Verfahren und vorrichtung zur echtzeiterfassung der produkte von nukleinsäureamplifikation |
| WO1995032306A1 (en) * | 1994-05-23 | 1995-11-30 | Biotronics Corporation | Method for detecting a target nucleic acid |
| US5622821A (en) * | 1994-06-29 | 1997-04-22 | The Regents Of The University Of California | Luminescent lanthanide chelates and methods of use |
| DE69527125T2 (de) * | 1994-11-09 | 2003-02-27 | Hitachi, Ltd. | Verfahren und Vorrichtung zur DNS Analyse |
| US5800996A (en) * | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
-
1997
- 1997-06-04 AT AT04022352T patent/ATE390491T1/de not_active IP Right Cessation
- 1997-06-04 EP EP03025810A patent/EP1442794B1/en not_active Expired - Lifetime
- 1997-06-04 ES ES04022352T patent/ES2304573T3/es not_active Expired - Lifetime
- 1997-06-04 AT AT06004925T patent/ATE485397T1/de not_active IP Right Cessation
- 1997-06-04 WO PCT/US1997/009620 patent/WO1997046707A2/en not_active Ceased
- 1997-06-04 ES ES97926890T patent/ES2253778T3/es not_active Expired - Lifetime
- 1997-06-04 DE DE69727932T patent/DE69727932T2/de not_active Expired - Lifetime
- 1997-06-04 ES ES06004925T patent/ES2354902T3/es not_active Expired - Lifetime
- 1997-06-04 CA CA2256612A patent/CA2256612C/en not_active Expired - Lifetime
- 1997-06-04 EP EP06004925A patent/EP1674585B1/en not_active Expired - Lifetime
- 1997-06-04 CA CA2256773A patent/CA2256773C/en not_active Expired - Lifetime
- 1997-06-04 NZ NZ333137A patent/NZ333137A/xx not_active IP Right Cessation
- 1997-06-04 AT AT97929833T patent/ATE260988T1/de not_active IP Right Cessation
- 1997-06-04 DE DE69740030T patent/DE69740030D1/de not_active Expired - Lifetime
- 1997-06-04 ES ES97929833T patent/ES2215230T3/es not_active Expired - Lifetime
- 1997-06-04 DE DE69738605T patent/DE69738605T2/de not_active Expired - Lifetime
- 1997-06-04 EP EP97926890A patent/EP0912760B1/en not_active Expired - Lifetime
- 1997-06-04 DE DE69734587T patent/DE69734587T2/de not_active Expired - Lifetime
- 1997-06-04 NZ NZ333135A patent/NZ333135A/en unknown
- 1997-06-04 AT AT97926890T patent/ATE309388T1/de active
- 1997-06-04 EP EP04022352A patent/EP1493826B1/en not_active Expired - Lifetime
- 1997-06-04 JP JP50080798A patent/JP4278712B2/ja not_active Expired - Lifetime
- 1997-06-04 ES ES10174616T patent/ES2434258T3/es not_active Expired - Lifetime
- 1997-06-04 DE DE69735563T patent/DE69735563T2/de not_active Expired - Lifetime
- 1997-06-04 DK DK10174616.2T patent/DK2298931T3/da active
- 1997-06-04 PT PT101746162T patent/PT2298931E/pt unknown
- 1997-06-04 ES ES03025810T patent/ES2260562T3/es not_active Expired - Lifetime
- 1997-06-04 JP JP50087498A patent/JP4281877B2/ja not_active Expired - Lifetime
- 1997-06-04 WO PCT/US1997/009856 patent/WO1997046712A2/en not_active Ceased
- 1997-06-04 EP EP05020549A patent/EP1704922A3/en not_active Withdrawn
- 1997-06-04 EP EP10174616.2A patent/EP2298931B2/en not_active Expired - Lifetime
- 1997-06-04 AU AU31547/97A patent/AU727296B2/en not_active Expired
- 1997-06-04 AU AU33800/97A patent/AU729644B2/en not_active Expired
- 1997-06-04 AT AT03025810T patent/ATE321891T1/de not_active IP Right Cessation
- 1997-06-04 EP EP97929833A patent/EP0906449B1/en not_active Expired - Lifetime
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2304573T3 (es) | Recipiente para llevar a cabo y controlar procesos biologicos. | |
| US7273749B1 (en) | Container for carrying out and monitoring biological processes | |
| US7081226B1 (en) | System and method for fluorescence monitoring | |
| ES2871861T3 (es) | Sistema de amplificación por reacción en cadena de la polimerasa mediada por luz y de detección de productos y métodos de uso | |
| Wittwer et al. | The LightCyclerTM: a microvolume multisample fluorimeter with rapid temperature control | |
| ES2248121T3 (es) | Sistema para termociclacion de fluidos en cartuchos. | |
| KR100479782B1 (ko) | 생물학적공정모니터링방법및시스템 | |
| JPWO2004068144A1 (ja) | 液体試料の分析方法及び分析装置 | |
| KR100479767B1 (ko) | 형광에의한dna증폭모니터링방법 | |
| Chien et al. | Simultaneous quantification for Hepatitis B virus and Hepatitis C virus using real-time PCR Lab-on-a-chip | |
| Tan et al. | A miniaturized fluorescence imaging system for detecting disease genes on a micro PCR biochip | |
| WO2004055499A1 (en) | Assay apparatus |