CA2256773C - System and process for carrying out and monitoring polymerase chain reaction - Google Patents

System and process for carrying out and monitoring polymerase chain reaction Download PDF

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Publication number
CA2256773C
CA2256773C CA2256773A CA2256773A CA2256773C CA 2256773 C CA2256773 C CA 2256773C CA 2256773 A CA2256773 A CA 2256773A CA 2256773 A CA2256773 A CA 2256773A CA 2256773 C CA2256773 C CA 2256773C
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CA
Canada
Prior art keywords
sample
temperature
fluorescence
reaction
monitoring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CA2256773A
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English (en)
French (fr)
Other versions
CA2256773A1 (en
Inventor
Carl T. Wittwer
Kirk M. Ririe
Randy P. Rasmussen
David R. Hillyard
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University of Utah Research Foundation Inc
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University of Utah Research Foundation Inc
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Application filed by University of Utah Research Foundation Inc filed Critical University of Utah Research Foundation Inc
Publication of CA2256773A1 publication Critical patent/CA2256773A1/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6452Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6823Release of bound markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0832Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838Capillaries
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/18Means for temperature control
    • B01L2300/1838Means for temperature control using fluid heat transfer medium
    • B01L2300/1844Means for temperature control using fluid heat transfer medium using fans
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N2021/6482Sample cells, cuvettes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178Special arrangements of analysers
    • G01N2035/00237Handling microquantities of analyte, e.g. microvalves, capillary networks
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03Cuvette constructions
    • G01N21/0303Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03Cuvette constructions
    • G01N21/07Centrifugal type cuvettes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Selective Calling Equipment (AREA)
  • Microscoopes, Condenser (AREA)
  • Control Of Temperature (AREA)
CA2256773A 1996-06-04 1997-06-04 System and process for carrying out and monitoring polymerase chain reaction Expired - Lifetime CA2256773C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US65899396A 1996-06-04 1996-06-04
US08/658,993 1996-06-04
PCT/US1997/009856 WO1997046712A2 (en) 1996-06-04 1997-06-04 System and method for carrying out and monitoring biological processes

Publications (2)

Publication Number Publication Date
CA2256773A1 CA2256773A1 (en) 1997-12-11
CA2256773C true CA2256773C (en) 2010-01-26

Family

ID=24643601

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2256612A Expired - Lifetime CA2256612C (en) 1996-06-04 1997-06-04 System and methods for monitoring for dna amplification by fluorescence
CA2256773A Expired - Lifetime CA2256773C (en) 1996-06-04 1997-06-04 System and process for carrying out and monitoring polymerase chain reaction

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA2256612A Expired - Lifetime CA2256612C (en) 1996-06-04 1997-06-04 System and methods for monitoring for dna amplification by fluorescence

Country Status (11)

Country Link
EP (7) EP1704922A3 (enExample)
JP (2) JP4281877B2 (enExample)
AT (5) ATE260988T1 (enExample)
AU (2) AU727296B2 (enExample)
CA (2) CA2256612C (enExample)
DE (5) DE69727932T2 (enExample)
DK (1) DK2298931T3 (enExample)
ES (6) ES2215230T3 (enExample)
NZ (2) NZ333137A (enExample)
PT (1) PT2298931E (enExample)
WO (2) WO1997046707A2 (enExample)

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EP2298931A1 (en) 2011-03-23
DE69734587D1 (de) 2005-12-15
ES2354902T3 (es) 2011-03-21
EP1493826B1 (en) 2008-03-26
EP0912760A2 (en) 1999-05-06
EP2298931B1 (en) 2013-08-07
DE69735563T2 (de) 2007-05-16
EP1704922A3 (en) 2007-12-12
JP4281877B2 (ja) 2009-06-17
AU727296B2 (en) 2000-12-07
EP1442794A3 (en) 2005-05-11
CA2256612C (en) 2012-09-25
AU3380097A (en) 1998-01-05
ES2260562T3 (es) 2006-11-01
CA2256612A1 (en) 1997-12-11
NZ333137A (en) 2000-03-27
EP1704922A2 (en) 2006-09-27
EP1442794B1 (en) 2006-03-29
ATE309388T1 (de) 2005-11-15
PT2298931E (pt) 2013-11-11
EP0912760B1 (en) 2005-11-09
DK2298931T3 (da) 2013-11-11
EP2298931A9 (en) 2011-06-01
WO1997046707A2 (en) 1997-12-11
ATE321891T1 (de) 2006-04-15
CA2256773A1 (en) 1997-12-11
DE69727932T2 (de) 2005-03-10
JP2000512138A (ja) 2000-09-19
ES2215230T3 (es) 2004-10-01
ES2253778T3 (es) 2006-06-01
JP2000511435A (ja) 2000-09-05
EP1674585A1 (en) 2006-06-28
ATE390491T1 (de) 2008-04-15
DE69738605T2 (de) 2009-04-30
ES2434258T3 (es) 2013-12-16
AU3154797A (en) 1998-01-05
ATE260988T1 (de) 2004-03-15
WO1997046712A2 (en) 1997-12-11
EP2298931B2 (en) 2020-06-24
JP4278712B2 (ja) 2009-06-17
DE69740030D1 (de) 2010-12-02
DE69738605D1 (de) 2008-05-08
ATE485397T1 (de) 2010-11-15
WO1997046707A3 (en) 1998-10-08
DE69735563D1 (de) 2006-05-18
EP1674585B1 (en) 2010-10-20
DE69727932D1 (de) 2004-04-08
WO1997046712A3 (en) 1998-05-07
NZ333135A (en) 2000-02-28
EP0906449A2 (en) 1999-04-07
EP1442794A2 (en) 2004-08-04
EP0906449B1 (en) 2004-03-03
AU729644B2 (en) 2001-02-08
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DE69734587T2 (de) 2006-07-20
EP1493826A1 (en) 2005-01-05

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