ES2116578T3 - Procedimiento y dispositivo para evaluar la aptitud de biopolimeros. - Google Patents

Procedimiento y dispositivo para evaluar la aptitud de biopolimeros.

Info

Publication number
ES2116578T3
ES2116578T3 ES94905081T ES94905081T ES2116578T3 ES 2116578 T3 ES2116578 T3 ES 2116578T3 ES 94905081 T ES94905081 T ES 94905081T ES 94905081 T ES94905081 T ES 94905081T ES 2116578 T3 ES2116578 T3 ES 2116578T3
Authority
ES
Spain
Prior art keywords
molecules
aptitude
biopolymers
evaluate
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94905081T
Other languages
English (en)
Inventor
Rudolf Rigler
Manfred Eigen
Karsten Henco
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Evotec Biosystems GmbH
Original Assignee
Evotec Biosystems GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27204658&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2116578(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from DE4301005A external-priority patent/DE4301005A1/de
Application filed by Evotec Biosystems GmbH filed Critical Evotec Biosystems GmbH
Application granted granted Critical
Publication of ES2116578T3 publication Critical patent/ES2116578T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/808—Optical sensing apparatus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Microscoopes, Condenser (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Electrotherapy Devices (AREA)
  • Image Analysis (AREA)

Abstract

LA INVENCION SE REFIERE A UN PROCEDIMIENTO PARA LA INDICACION DE UNO O UN PEQUEÑO NUMERO DE MOLECULAS, EN PARTICULAR EN UNA DILUCION MENOR O IGUAL A 1 MICRA, MEDIANTE LA UTILIZACION DE CORRELACION ELECTROSCOPICA DE FLUORESCENCIA SIMULADA LASER CON TIEMPOS DE MEDICION DE MENOR O IGUAL A 500 MS Y VIAS DE DIFUSION CORTAS PARA LA MOLECULAS BAJO ANALISIS, SIENDO REALIZADO CON PREFERENCIA LA MEDICION SOBRE VOLUMENES PEQUEÑOS DE MENOR O IGUAL A 10 MENOS{SUP.14} LITROS PARA LA MEDICION ESPECIFICA DE LOS PARAMETROS DEL MATERIAL Y QUE PUEDEN SER DETERMINADOS MEDIANTE MEDICIONES DE LUMINISCENCIA SOBRE LAS MOLECULAS EN INVESTIGACION. EL DISPOSITIVO SE UTILIZA CON PREFERENCIA PARA LA REALIZACION DEL METODO PROPUESTO Y DISPONE POR SI MISMO DE OPTICAS MICROSCOPICAS DE TIPO CONOCIDO QUE FOCALIZAN LA LUZ LASER UTILIZADA PARA ESTIMULAR LA FLUORESCENCIA DE UNA SOLUCION ALTAMENTE DIVIDIDA EN UNA CELULA DE DIVISION PEQUEÑA Y PARA FORMA UNA IMAGEN A PARTIR DE LA LUZ EMITIDA EN LA ETAPA DE MEDICION SUBSECUENTE MEDIANTE DISPOSICION DE IMAGEN CONFOCAL. LA OPTICA UTILIZADA DISPONE DE UNA APERTURA NUMERICA GRANDE DE MAYOR O IGUAL A 1,2 N.A., LA CANTIDAD DE LA LUZ ESTA LIMITADA POR MEDIO DE UN DIAFRAGMA DE APERTURA DISPUESTO DE FORMA CONFOCAL CON EL PLANO DEL OBJETO DESPUES DE LAS LENTES OBJETIVO DEL MICROSCOPIO, Y LA CELDA DE MEDICION ESTA POSICIONADA A UNA DISTANCIA ENTRE 0 Y 1.000 MICRAS A PARTIR DE LAS LENTES DE OBSERVACION.
ES94905081T 1993-01-18 1994-01-18 Procedimiento y dispositivo para evaluar la aptitud de biopolimeros. Expired - Lifetime ES2116578T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4301005A DE4301005A1 (de) 1993-01-18 1993-01-18 Verfahren und Vorrichtung zur Bewertung der Fitness von Biopolymeren
EP93912765 1993-05-22
DE4342703 1993-12-15

Publications (1)

Publication Number Publication Date
ES2116578T3 true ES2116578T3 (es) 1998-07-16

Family

ID=27204658

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94905081T Expired - Lifetime ES2116578T3 (es) 1993-01-18 1994-01-18 Procedimiento y dispositivo para evaluar la aptitud de biopolimeros.

Country Status (9)

Country Link
US (1) US6582903B1 (es)
EP (1) EP0679251B1 (es)
JP (1) JP3517241B2 (es)
AT (1) ATE164943T1 (es)
AU (1) AU5884394A (es)
DE (1) DE59405644D1 (es)
DK (1) DK0679251T3 (es)
ES (1) ES2116578T3 (es)
WO (1) WO1994016313A2 (es)

Families Citing this family (126)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7241569B2 (en) * 1993-01-18 2007-07-10 Olympus Corporation Method and a device for the evaluation of biopolymer fitness
EP0765470A2 (de) * 1994-06-17 1997-04-02 Evotec BioSystems GmbH Verfahren und vorrichtung zur gezielten entnahme von komponenten aus komplexen mischungen
DE4438391C2 (de) * 1994-10-27 1997-07-03 Evotec Biosystems Gmbh Vorrichtung zur Bestimmung stoffspezifischer Parameter eines oder weniger Moleküle mittels Korrelations-Spektroskopie
DE69615818T2 (de) * 1995-01-16 2002-06-06 Erkki Soini Ein biospezifisches multiparametrisches testverfahren
FI101829B (fi) * 1995-03-07 1998-08-31 Erkki Juhani Soini Biospesifinen määritysmenetelmä
FI98765C (fi) 1995-01-16 1997-08-11 Erkki Soini Virtaussytometrinen menetelmä ja laite
DE19604363A1 (de) * 1995-02-23 1996-08-29 Zeiss Carl Fa Zusatzmodul zur ortsaufgelösten Fokusvermessung
DE19508366C2 (de) * 1995-03-10 1998-01-29 Evotec Biosystems Gmbh Verfahren zum direkten Nachweisen weniger Nucleinsäurestränge
DE19545965A1 (de) * 1995-12-08 1997-06-12 Max Planck Gesellschaft Verfahren zur Bestimmung von Nukleinsäuremolekülen in niedriger Konzentration
DE19648458C1 (de) * 1996-11-22 1998-07-09 Evotec Biosystems Gmbh Mikromechanische Ejektionspumpe zum Heraustrennen kleinster Fluidvolumina aus einem strömenden Probenfluid
DE19702914C2 (de) * 1997-01-28 1998-12-24 Max Planck Gesellschaft Verfahren und Anordnung zum Bestimmen vorgegebener Eigenschaften von Zielpartikeln eines Probenmediums
DE19649048C1 (de) * 1996-11-27 1998-04-09 Evotec Biosystems Gmbh Verfahren zur Unterscheidung oder Erfassung von Partikeln in einer Probe durch Identifizierung von Signalabschnitten zeitaufgelöster, optischer Rohsignale aus der Probe auf Basis von Einzelphotonendetektion
DE19649605A1 (de) * 1996-11-29 1998-06-04 Deutsches Krebsforsch Fluoreszenzkorrelationsspektroskopiemodul für ein Mikroskop
US6361956B1 (en) 1996-12-03 2002-03-26 Erkki Soini Biospecific, two photon excitation, fluorescence detection and device
US6310354B1 (en) 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
DE19723873B4 (de) * 1997-06-06 2004-02-05 Evotec Oai Ag Verfahren und Vorrichtung zur Bewegungserfassung eines sich zumindest zeitweilig periodisch bewegenden Objekts
EP0884583A1 (en) * 1997-06-10 1998-12-16 Evotec BioSystems GmbH A method for characterizing samples in at least two dimensional space of specific physical properties
DE19729245C1 (de) * 1997-07-09 1999-05-06 Evotec Biosystems Ag Spiegelobjektiv und dessen Verwendung
DE19735119C2 (de) 1997-08-13 2000-05-18 Deutsches Krebsforsch Verfahren zur Fluoreszenzkorrelationsspektroskopie, insbesbesondere zur Mehrfarben-Fluoreszenzkorrelationsspektroskopie und Vorrichtung zur Durchführung des Verfahrens
US6493459B2 (en) 1997-11-06 2002-12-10 Fuji Photo Film Co., Ltd. Image reading apparatus
DE19757740C2 (de) * 1997-12-23 2000-04-13 Evotec Biosystems Ag Verfahren zum Nachweis von Assoziations-, Dissoziations-, Verknüpfungs- oder Spaltreaktionen sowie Konformationsänderungen mittels Koinzidenzanalyse
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
DE19903576C2 (de) 1999-01-29 2001-02-22 Bodenseewerk Perkin Elmer Co Quantitative Bestimmung von Analyten in einem heterogenen System
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
DE19951188B4 (de) * 1999-10-22 2013-04-25 Carl Zeiss Microscopy Gmbh Verfahren und Einrichtung zur Aufzeichnung von Impulssignalen
DE10008594B4 (de) * 2000-02-22 2018-09-20 Carl Zeiss Microscopy Gmbh Einrichtung und Verfahren zur ortsaufgelösten Fluoreszenz-Korrelations-Spektroskopie
DE10013854A1 (de) * 2000-03-17 2001-09-20 Zeiss Carl Jena Gmbh Anordnung und Verfahren zur Untersuchung von Wirkstoffen zur Beeinflussung intrazellulärer Vorgänge
DE60126711T2 (de) * 2000-03-27 2007-10-25 Olympus Co. Verfahren zum messen von polymorphismen
JP3910000B2 (ja) * 2000-03-27 2007-04-25 オリンパス株式会社 １塩基置換検出方法
JP2001269198A (ja) * 2000-03-27 2001-10-02 Olympus Optical Co Ltd 多型遺伝子の型を決定する方法
JP2001272404A (ja) * 2000-03-27 2001-10-05 Olympus Optical Co Ltd 蛍光相関分光法による抗原抗体反応
JP4589480B2 (ja) * 2000-03-30 2010-12-01 オリンパス株式会社 反復配列の多型を分析する方法
FR2807543B1 (fr) * 2000-04-06 2004-11-05 Imstar S A Appareil d'imagerie associe a une base de donnees images
AU2001262284A1 (en) * 2000-05-12 2001-11-20 Gnothis Holding Sa Method for detecting polynucleotides using fluorescence correlation spectroscopy
DE10065632A1 (de) 2000-05-12 2001-11-15 Smtech Biovision Holding Ag Ec Verfahren zum Nachweis von Polynukleotiden
DE10023423B4 (de) * 2000-05-12 2009-03-05 Gnothis Holding Sa Direkter Nachweis von Einzelmolekülen
DE10031028B4 (de) * 2000-06-26 2008-09-04 Gnothis Holding Sa Verfahren zur Selektion von Partikeln
DE10035190C5 (de) * 2000-07-20 2009-07-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren und Vorrichtung zur Fluoreszenzmessung
US6603546B1 (en) * 2000-07-21 2003-08-05 I.S.S. (Usa) Inc. Rapid high throughput spectrometer and method
DE10040988A1 (de) 2000-08-22 2002-03-21 Evotec Biosystems Ag Verfahren und Vorrichtung zum Messen chemischer und/oder biologischer Proben
DE50114075D1 (de) * 2000-08-22 2008-08-14 Evotec Ag Verfahren und vorrichtung zum messen chemischer und/oder biologischer proben
DE10103304A1 (de) * 2001-01-25 2002-08-01 Gnothis Holding Sa Ecublens Verfahren zur fluoreszenzspektroskopischen, insbesondere fluoreszenzkorrelationsspektroskopischen Untersuchung einer Messprobe sowie Einrichtung zur Durchführung desselben
WO2002073172A2 (de) * 2001-03-09 2002-09-19 Gnothis Holding Sa Bestimmung von analyten durch fluoreszenz-korrelationsspektroskopie
DE10111420A1 (de) * 2001-03-09 2002-09-12 Gnothis Holding Sa Ecublens Bestimmung von Analyten durch Fluoreszenz-Korrelationsspektroskopie
DE10116610B4 (de) * 2001-04-03 2004-04-15 Evotec Oai Ag Verfahren zum Messen chemischer und/oder biologischer Proben mit Hilfe der Lumineszenz-Spektroskopie
JP2002310894A (ja) * 2001-04-11 2002-10-23 Japan Science & Technology Corp 発光観測装置、発光体選別装置、及び発光体の選別方法
WO2002090947A2 (de) * 2001-05-07 2002-11-14 Deutsches Krebsforschungszentrum Stiftung D. Öffentl. Rechts Fluoreszenzfluktuationsmikroskop, -messmodul bzw.-scanmodul und verfahren zur fluoreszenzfluktuationsmessung sowie verfahren und vorrichtung zur justage eines fluoreszenzfluktuationsmikroskops
DE10126083A1 (de) 2001-05-29 2002-12-05 Gnothis Holding Sa Ecublens Verwendung von optischen Diffraktionselementen in Nachweisverfahren
EP1421367A1 (de) 2001-08-28 2004-05-26 Gnothis Holding SA Einkanal-mehrfarben-korrelationsanalyse
MXPA04003926A (es) * 2001-10-24 2004-06-18 Singulex Inc Metodos para detectar haplotipos geneticos por interaccion con sondas.
US20040028559A1 (en) * 2001-11-06 2004-02-12 Peter Schuck Sample delivery system with laminar mixing for microvolume biosensing
JP2003177131A (ja) * 2001-12-11 2003-06-27 Olympus Optical Co Ltd 生物学的な結合親和性を検出する方法
DE10221564A1 (de) * 2002-05-15 2003-11-27 Evotec Ag Vorrichtung und Verfahren zur Untersuchung chemischer und/oder biologischer Proben
EP1546675A4 (en) * 2002-08-01 2008-03-26 Sensor Technologies Inc METHOD FOR MEASURING MOLECULAR INTERACTIONS
JP4002818B2 (ja) * 2002-11-21 2007-11-07 オリンパス株式会社 測定装置
US7400396B2 (en) * 2003-02-13 2008-07-15 Hamanatsu Photonics K.K. Fluorescent correalated spectrometric analysis device
US7229763B2 (en) * 2003-04-07 2007-06-12 Beckman Coulter, Inc. Assay system using labeled oligonucleotides
EP1617221A4 (en) * 2003-04-23 2006-08-02 Olympus Corp METHOD FOR SCREENING A SUBSTANCE CAPABLE OF BINDING TO A RECEIVER
JP2004347562A (ja) * 2003-05-26 2004-12-09 Olympus Corp 測定装置
US20040265171A1 (en) * 2003-06-27 2004-12-30 Pugia Michael J. Method for uniform application of fluid into a reactive reagent area
US7672786B2 (en) * 2003-07-02 2010-03-02 Sergey Krylov Non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM)—based methods for drug and diagnostic development
WO2005019419A2 (en) * 2003-07-31 2005-03-03 Singulex, Inc. Co-detection of single polypeptide and polynucleotide molecules
DE102004003993A1 (de) * 2003-08-12 2005-03-17 Leica Microsystems Heidelberg Gmbh Vorrichtung zum Nachweis von Photonen eines Lichtstrahls
EP1664889B1 (de) * 2003-08-12 2010-10-06 Leica Microsystems CMS GmbH Vorrichtung zum nachweis von photonen eines lichtstrahls
JP2005069973A (ja) * 2003-08-27 2005-03-17 Kyocera Corp 遺伝子反応管および遺伝子検出装置
JP2005083980A (ja) * 2003-09-10 2005-03-31 Olympus Corp 共焦点光学顕微鏡およびそれを用いた分析方法
US20080021674A1 (en) * 2003-09-30 2008-01-24 Robert Puskas Methods for Enhancing the Analysis of Particle Detection
DE10350918B3 (de) * 2003-10-31 2005-04-14 Evotec Technologies Gmbh Vorrichtung und Verfahren zur Messung der Transmission eines Objekts
ES2237319B1 (es) * 2003-11-27 2006-12-16 Universidad De Barcelona Metodo de identificacion de pigmentos de una sola celula mediante espectrofotometria de imagen confocal en comunidades fototroficas.
JP4338590B2 (ja) * 2004-06-03 2009-10-07 独立行政法人科学技術振興機構 蛍光相関分光法による抗原の迅速検出及び／又は測定法。
JP4914715B2 (ja) 2004-06-21 2012-04-11 オリンパス株式会社 倒立顕微鏡システム
JP2006017628A (ja) * 2004-07-02 2006-01-19 Olympus Corp 蛍光分光分析装置及び蛍光分光分析方法
US7276720B2 (en) * 2004-07-19 2007-10-02 Helicos Biosciences Corporation Apparatus and methods for analyzing samples
JP4425098B2 (ja) * 2004-09-06 2010-03-03 浜松ホトニクス株式会社 蛍光顕微鏡および蛍光相関分光解析装置
US9040305B2 (en) * 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
US8685711B2 (en) * 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US20060078998A1 (en) * 2004-09-28 2006-04-13 Singulex, Inc. System and methods for sample analysis
US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties
DE102004047953A1 (de) * 2004-10-01 2006-04-20 Rudolf Rigler Selektion von Partikeln im laminaren Fluss
US20070070349A1 (en) * 2005-09-23 2007-03-29 Helicos Biosciences Corporation Optical train and method for TIRF single molecule detection and analysis
JP2006208240A (ja) * 2005-01-28 2006-08-10 Nippon Sheet Glass Co Ltd 光学測定用基板および該基板の作製方法
EP1726643A1 (en) 2005-05-27 2006-11-29 Direvo Biotech AG Method for the provision, identification and selection of proteases with altered sensitivity to activity-modulating substances
JP4517980B2 (ja) * 2005-08-23 2010-08-04 株式会社島津製作所 粒子測定装置
EP1757969A1 (en) * 2005-08-24 2007-02-28 Olympus Corporation Microscope moving unit and microscope apparatus
WO2007032266A1 (ja) 2005-09-12 2007-03-22 Japan Science And Technology Agency 蛍光相関分光法又は蛍光相互相関分光法を用いた抗原の迅速検出法
JP4650277B2 (ja) * 2006-01-17 2011-03-16 株式会社島津製作所 粒径計測装置
US7838250B1 (en) 2006-04-04 2010-11-23 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
WO2007114947A2 (en) 2006-04-04 2007-10-11 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
WO2008009673A2 (en) 2006-07-18 2008-01-24 Direvo Industrial Biotechnology Gmbh Mannanases
WO2008085991A2 (en) 2007-01-08 2008-07-17 U.S. Genomics, Inc. Reaction chamber
DE102007033737A1 (de) * 2007-07-18 2009-01-22 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zum Bestimmen eines Messwerts auf der Basis von Einzelmolekülereignissen
AU2008352940B2 (en) 2007-12-19 2014-06-05 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
CN102016552A (zh) * 2008-03-05 2011-04-13 神谷来克斯公司 用于分子的高灵敏性检测的方法和组合物
US8361716B2 (en) 2008-10-03 2013-01-29 Pathogenetix, Inc. Focusing chamber
EP2186827A1 (en) 2008-11-14 2010-05-19 HS LifeSciences Ltd. Surrogate marker directed cDNA cloning of selectively induced mRNAs
US8409839B2 (en) 2008-12-10 2013-04-02 Direvo Industrial Biotechnology Gmbh Polypeptides having cellobiohydrolase II activity
AU2010259022B2 (en) * 2009-06-08 2016-05-12 Singulex, Inc. Highly sensitive biomarker panels
DE102010012580A1 (de) * 2010-03-23 2011-09-29 Hochschule Niederrhein Vorrichtung und Verfahren zur zeitaufgelösten Durchflusszytometrie
US20110301053A1 (en) 2010-05-06 2011-12-08 Singulex, Inc. Methods for Diagnosing, Staging, Predicting Risk for Developing and Identifying Treatment Responders for Rheumatoid Arthritis
JP5876045B2 (ja) 2010-07-12 2016-03-02 ユニバーシティー オブ サザン カリフォルニア 幹細胞と標的組織の相互接続を促進する生体適合性基材およびそれを埋め込む方法
CN103097878B (zh) 2010-09-10 2015-07-22 奥林巴斯株式会社 使用单个发光颗粒的光强度的光学分析方法
WO2012032955A1 (ja) 2010-09-10 2012-03-15 オリンパス株式会社 複数の波長帯域の光計測を用いた光分析方法
EP2631631B1 (en) 2010-11-25 2016-01-20 Olympus Corporation Photometric analysis device and photometric analysis method using wavelength characteristic of light emitted from single illuminant particle
US8877489B2 (en) 2011-12-05 2014-11-04 California Institute Of Technology Ultrathin parylene-C semipermeable membranes for biomedical applications
WO2012149468A2 (en) 2011-04-29 2012-11-01 University Of Southern California Instruments and methods for the implantation of cell-seeded substrates
US9248013B2 (en) 2011-12-05 2016-02-02 California Institute Of Technology 3-Dimensional parylene scaffold cage
US9588113B2 (en) 2012-02-22 2017-03-07 Church & Dwight Co., Inc. Methods for electronic analyte assaying
US9920364B2 (en) 2012-03-06 2018-03-20 Rudolf Rigler Cyclic single molecule sequencing process
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
DE102013213362A1 (de) 2013-07-08 2015-01-08 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur Identifizierung und Quantifizierung von emittierenden Teilchen in Systemen
WO2015104245A1 (en) 2014-01-10 2015-07-16 Gnothis Holding Ag Single molecule analysis with high accuracy
US9867250B1 (en) 2015-04-20 2018-01-09 The Arizona Board Of Regents On Behalf Of The University Of Arizona System having a configurable pico-second pulsed LED driver circuit and photomultiplier tube biasing and gating circuits for real-time, time-resolved transient recording of fluorescence
EP3112841A1 (en) 2015-06-30 2017-01-04 Gnothis Holding SA Single molecule analysis in an electrical field
DE102017105113B4 (de) 2017-03-10 2021-09-30 Leibniz-Institut für Photonische Technologien e.V. (Engl.Leibniz Institute of Photonic Technology) Anordnung und Verfahren für die Erfassung von Änderungen der optischen Weglänge in einem Nano-Kapillarkanal
US20220220430A1 (en) * 2019-05-23 2022-07-14 Bristol-Myers Squibb Company Methods of monitoring cell culture media
US12013521B2 (en) 2020-06-29 2024-06-18 Mgi Tech Co., Ltd. Systems and methods for optical scanning and imaging through a fluid medium for nucleic acid sequencing
CN113495402B (zh) * 2021-07-06 2022-06-14 宁波胤瑞生物医学仪器有限责任公司 自动对焦装置
EP4379378A1 (en) 2022-11-29 2024-06-05 Gnothis Holding AG Selective-area coated protein resistant surface
WO2024099959A1 (en) 2022-11-07 2024-05-16 Gnothis Holding Ag Selective-area coated protein resistant surface
EP4403643A1 (en) 2023-01-19 2024-07-24 Gnothis Holding AG Multiple molecular binding sites
WO2024110463A1 (en) 2022-11-21 2024-05-30 Gnothis Holding Ag Multiple molecular binding sites
EP4375644A1 (en) 2022-11-22 2024-05-29 Gnothis Holding AG Fingerprint analysis of single molecule events
EP4379355A1 (en) 2022-11-29 2024-06-05 Gnothis Holding AG High throughput analysis of single molecule events
EP4382614A1 (en) 2022-12-06 2024-06-12 Gnothis Holding AG High yield support
US20240302260A1 (en) * 2023-03-10 2024-09-12 Solaris Biosciences, Inc. Optical systems and methods of probing biological fluids

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3872312A (en) * 1973-07-02 1975-03-18 Block Engineering Method and apparatus for detecting and classifying nucleic acid particles
US4405237A (en) * 1981-02-04 1983-09-20 The United States Of America As Represented By The Secretary Of The Navy Coherent anti-Stokes Raman device
EP0245206A1 (en) * 1986-05-05 1987-11-11 IntraCel Corporation Analytical method for detecting and measuring specifically sequenced nucleic acid
CA1341055C (en) * 1987-12-08 2000-07-18 Alan Mcclelland Transfectant cell lines which express the major human rhinovirus receptor
US5720928A (en) * 1988-09-15 1998-02-24 New York University Image processing and analysis of individual nucleic acid molecules
US5252743A (en) * 1989-11-13 1993-10-12 Affymax Technologies N.V. Spatially-addressable immobilization of anti-ligands on surfaces
DE4022792A1 (de) * 1990-07-18 1992-02-06 Max Planck Gesellschaft Platte mit zumindest einer mulde zur aufnahme von chemischen und/oder biochemischen und/oder mikrobiologischen substanzen und verfahren zur herstellung der platte
EP0501688A1 (en) * 1991-02-27 1992-09-02 Hitachi, Ltd. Apparatus and method for applying a laser beam through a microscope
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
GB9218482D0 (en) * 1992-09-01 1992-10-14 Dixon Arthur E Apparatus and method for scanning laser imaging of macroscopic samples
US5381224A (en) * 1993-08-30 1995-01-10 A. E. Dixon Scanning laser imaging system
US5608519A (en) * 1995-03-20 1997-03-04 Gourley; Paul L. Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells

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ATE164943T1 (de) 1998-04-15
EP0679251A1 (de) 1995-11-02
US6582903B1 (en) 2003-06-24
DE59405644D1 (de) 1998-05-14
WO1994016313A3 (de) 1994-09-29
JP3517241B2 (ja) 2004-04-12
EP0679251B1 (de) 1998-04-08
AU5884394A (en) 1994-08-15
WO1994016313A2 (de) 1994-07-21
DK0679251T3 (da) 1999-01-25

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