ES2009286A6 - Metodos para preparar acido nucleico bicatenario con una secuencia objetivo, detectar dicha secuencia y multiplicar un segmento de acido nucleico objetivo, y estuche de deteccion correspondiente. - Google Patents

Metodos para preparar acido nucleico bicatenario con una secuencia objetivo, detectar dicha secuencia y multiplicar un segmento de acido nucleico objetivo, y estuche de deteccion correspondiente.

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Publication number
ES2009286A6
ES2009286A6 ES888801899A ES8801899A ES2009286A6 ES 2009286 A6 ES2009286 A6 ES 2009286A6 ES 888801899 A ES888801899 A ES 888801899A ES 8801899 A ES8801899 A ES 8801899A ES 2009286 A6 ES2009286 A6 ES 2009286A6
Authority
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Spain
Prior art keywords
nucleic acid
primer
transcription
duplex
acid amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES888801899A
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English (en)
Inventor
Thomas Raymond Gingeras
Ulrich Merten
Deborah Yantis Kwoh
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Siska Diagnostics Inc
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Siska Diagnostics Inc
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26744190&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2009286(A6) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Siska Diagnostics Inc filed Critical Siska Diagnostics Inc
Publication of ES2009286A6 publication Critical patent/ES2009286A6/es
Expired legal-status Critical Current

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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6867Replicase-based amplification, e.g. using Q-beta replicase
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6865Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/702Specific hybridization probes for retroviruses
    • C12Q1/703Viruses associated with AIDS
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

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  • Chemical & Material Sciences (AREA)
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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Radar Systems Or Details Thereof (AREA)
  • Indicating And Signalling Devices For Elevators (AREA)
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Abstract

METODOS PARA PREPARAR ACIDO NUCLEICO BICATENARIO CON UNA SECUENCIA OBJETIVO, DETECTAR DICHA SECUENCIA Y MULTIPLICAR UN SEGMENTO DE ACIDO NUCLEICO OBJETIVO, Y ESTUCHE DE DETECCION CORRESPONDIENTE. EL ACIDO NUCLEICO SE PREPARA HIBRIDANDO CON UNA SECUENCIA OBJETIVO Y PROLONGANDO UN PRIMER INICIADOR DEL MISMO, SEPARANDO LAS CADENAS DEL DUPLO FORMADO, E HIBRIDANDO CON LA CADENA SEPARADA Y PROLONGANDO UN SEGUNDO INICIADOR DEL ACIDO. LA DETECCION DE LA SECUENCIA OBJETIVO COMPRENDE EMPLEAR DICHO ACIDO NUCLEICO COMO PLANTILLA PARA OBTENER TRANSCRITOS CON UNA SECUENCIA DE ARN, Y DETECTAR ESTA SECUENCIA. EL METODO DE MULTIPLICACION COMPRENDE HIBRIDAR Y PROLONGAR INICIADORES PRIMERO T SEGUNDO DE ADN MONOCATENARIO, Y EMPLEAR EL PRODUCTO BICATENARIO OBTENIDO COMO PLANTILLA DE UNA REACCION CATALIZADA POR POLIMERASA. EL ESTUCHE INCLUYE INICIADORES PRIMERO Y SEGUNDO DE ACIDO NUCLEICO, Y MEDIOS PARA HIBRIDARLOS Y PROLONGARLOS, Y DETECTAR LOS TRANSCRITOS PRODUCIDOS. EL INVENTO ES UTIL PARA ANALIZAR PATOGENOSO GENES DEFICIENTES EN SANGRE.
ES888801899A 1987-06-19 1988-06-17 Metodos para preparar acido nucleico bicatenario con una secuencia objetivo, detectar dicha secuencia y multiplicar un segmento de acido nucleico objetivo, y estuche de deteccion correspondiente. Expired ES2009286A6 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6414187A 1987-06-19 1987-06-19
US20297888A 1988-06-06 1988-06-06

Publications (1)

Publication Number Publication Date
ES2009286A6 true ES2009286A6 (es) 1989-09-16

Family

ID=26744190

Family Applications (1)

Application Number Title Priority Date Filing Date
ES888801899A Expired ES2009286A6 (es) 1987-06-19 1988-06-17 Metodos para preparar acido nucleico bicatenario con una secuencia objetivo, detectar dicha secuencia y multiplicar un segmento de acido nucleico objetivo, y estuche de deteccion correspondiente.

Country Status (17)

Country Link
EP (2) EP0368906B2 (es)
JP (1) JP2843586B2 (es)
KR (1) KR960014107B1 (es)
AT (2) ATE114335T1 (es)
BR (1) BR8807097A (es)
DE (2) DE3856428T2 (es)
DK (1) DK175614B1 (es)
ES (1) ES2009286A6 (es)
FI (1) FI93743C (es)
GR (1) GR880100396A (es)
HU (1) HU216317B (es)
IE (1) IE65089B1 (es)
IL (1) IL86724A (es)
NO (1) NO303068B1 (es)
NZ (1) NZ225077A (es)
PT (1) PT87769B (es)
WO (1) WO1988010315A1 (es)

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FI896077A0 (fi) 1989-12-19
DK175614B1 (da) 2004-12-27
KR960014107B1 (ko) 1996-10-14
EP0368906B2 (en) 1999-08-04
IE881848L (en) 1988-12-19
HUT52823A (en) 1990-08-28
IE950293L (en) 1988-12-19
DE3856428T2 (de) 2001-04-19
WO1988010315A1 (en) 1988-12-29
PT87769B (pt) 1992-10-30
JP2843586B2 (ja) 1999-01-06
NO303068B1 (no) 1998-05-25
ATE196657T1 (de) 2000-10-15
KR890701741A (ko) 1989-12-21
DE3852177T2 (de) 1995-04-06
ATE114335T1 (de) 1994-12-15
NO895090L (no) 1990-02-19
DE3852177T3 (de) 2000-06-15
IL86724A0 (en) 1988-11-30
EP0623683B1 (en) 2000-09-27
IL86724A (en) 1995-01-24
FI93743B (fi) 1995-02-15
DK644489D0 (da) 1989-12-19
BR8807097A (pt) 1989-10-17
DK644489A (da) 1990-02-19
DE3856428D1 (de) 2000-11-02
EP0368906A1 (en) 1990-05-23
HU216317B (hu) 1999-06-28
JPH02500565A (ja) 1990-03-01
NO895090D0 (no) 1989-12-18
FI93743C (fi) 1995-05-26
EP0623683A1 (en) 1994-11-09
NZ225077A (en) 1991-06-25
PT87769A (pt) 1989-05-31

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