ES2092056T3 - Procedimiento para detectar arnm y adn especificos en celulas. - Google Patents

Procedimiento para detectar arnm y adn especificos en celulas.

Info

Publication number
ES2092056T3
ES2092056T3 ES92308190T ES92308190T ES2092056T3 ES 2092056 T3 ES2092056 T3 ES 2092056T3 ES 92308190 T ES92308190 T ES 92308190T ES 92308190 T ES92308190 T ES 92308190T ES 2092056 T3 ES2092056 T3 ES 2092056T3
Authority
ES
Spain
Prior art keywords
cells
screening
compounds
applicable
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92308190T
Other languages
English (en)
Inventor
Michael J Banker
Dennis A Pereira
Ralph E Davidson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Inc
Original Assignee
Pfizer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Inc filed Critical Pfizer Inc
Application granted granted Critical
Publication of ES2092056T3 publication Critical patent/ES2092056T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

ESTA INVENCION SE REFIERE A UN PROCESO PARA DETECTAR LA PRESENCIA Y MEDIR LA CANTIDAD DE SECUENCIAS DE MARN ESPECIFICAS PRESENTES EN CELULAS IN VIVO O EN CELULAS MANTENIDAS IN VITRO. EL PROCESO DE LA INVENCION SE PUEDE APLICAR PARA PROTEGER A ORGANISMOS PROCARIOTICOS Y EUCARIOTICOS INCLUIDA LA PROTECCION DE SERES HUMANOS CONTRA LA PRESENCIA DE ESTADOS ENFERMIZOS. EL PROCESO DE LA INVENCION SE PUEDE APLICAR TAMBIEN A LA PROTECCION IN VITRO CONTRA EL EFECTO O EFECTOS DE COMPUESTOS QUIMICOS EN UNO O VARIOS PRODUCTOS DE GENES SEGUN LO MUESTRE LA PRESENCIA Y CANTIDAD DE MARN RESULTANTE DE LA TRANSCRIPCION DE DICHO GEN O GENES. EL PROCESO DE ESTA INVENCION ES PARTICULARMENTE ADECUADO PROTEGER UN GRAN NUMERO DE COMPUESTO CONTRA EL EFECTO O EFECTOS DE LOS COMPUESTOS SOBRE LOS PRODUCTOS DE GENES. ESTA INVENCION SE REFIERE TAMBIEN A LOS COMPUESTOS CAPACES DE AFECTAR LA PRESENCIA DE SECUENCIAS DE MARN EN LAS CELULAS. EL PROCESO DE ESTA INVENCION TAMBIEN SE PUEDE APLICAR EN LA IDENTIFICACION DE NUEVASFORMACIONES DE GENES EN VIRUS, MICROORGANISMOS, PLANTAS Y ANIMALES. ESTA INVENCION SE REFIERE TAMBIEN A UN NUEVO PROCESO PARA AISLAR EL ARN O EL ADN DE LAS CELULAS A BASE DE LISIARLOS EN AGUA CALENTADA A 99 (GRADOS) C APROXIMADAMENTE.
ES92308190T 1991-09-23 1992-09-09 Procedimiento para detectar arnm y adn especificos en celulas. Expired - Lifetime ES2092056T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US76446291A 1991-09-23 1991-09-23

Publications (1)

Publication Number Publication Date
ES2092056T3 true ES2092056T3 (es) 1996-11-16

Family

ID=25070802

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92308190T Expired - Lifetime ES2092056T3 (es) 1991-09-23 1992-09-09 Procedimiento para detectar arnm y adn especificos en celulas.

Country Status (10)

Country Link
US (1) US5643730A (es)
EP (1) EP0534640B1 (es)
JP (1) JP2703156B2 (es)
AT (1) ATE143700T1 (es)
CA (1) CA2078703C (es)
DE (1) DE69214243T2 (es)
DK (1) DK0534640T3 (es)
ES (1) ES2092056T3 (es)
FI (1) FI103413B (es)
GR (1) GR3021721T3 (es)

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US6017496A (en) 1995-06-07 2000-01-25 Irori Matrices with memories and uses thereof
WO1997029211A1 (en) 1996-02-09 1997-08-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services RESTRICTION DISPLAY (RD-PCR) OF DIFFERENTIALLY EXPRESSED mRNAs
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US6573044B1 (en) 1997-08-07 2003-06-03 The Regents Of The University Of California Methods of using chemical libraries to search for new kinase inhibitors
EP1027607A1 (en) * 1997-10-31 2000-08-16 Sarnoff Corporation Method for enhancing fluorescence
US20100105572A1 (en) * 1997-12-19 2010-04-29 Kris Richard M High throughput assay system
US6458533B1 (en) 1997-12-19 2002-10-01 High Throughput Genomics, Inc. High throughput assay system for monitoring ESTs
US20030096232A1 (en) * 1997-12-19 2003-05-22 Kris Richard M. High throughput assay system
US6238869B1 (en) 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
US6232066B1 (en) 1997-12-19 2001-05-15 Neogen, Inc. High throughput assay system
US20030039967A1 (en) * 1997-12-19 2003-02-27 Kris Richard M. High throughput assay system using mass spectrometry
WO1999034018A1 (en) * 1997-12-24 1999-07-08 The Regents Of The University Of California Methods of using chemical libraries to search for new kinase inhibitors
WO1999050401A1 (fr) * 1998-03-27 1999-10-07 Helix Research Institute Procede pour detecter les changements de l'expression genique par le traitement avec un compose de test
US6448003B1 (en) * 1998-06-10 2002-09-10 Dna Sciences Laboratories, Inc. Genotyping the human phenol sulfotransferbase 2 gene STP2
CA2702148C (en) 1999-01-06 2014-03-04 Genenews Inc. Method of profiling gene expression in a human subject having an infectious disease
US7473528B2 (en) 1999-01-06 2009-01-06 Genenews Inc. Method for the detection of Chagas disease related gene transcripts in blood
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US20040185464A1 (en) * 2000-09-15 2004-09-23 Kris Richard M. High throughput assay system
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US7575864B2 (en) * 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
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JP2022102386A (ja) * 2020-12-25 2022-07-07 国立大学法人旭川医科大学 遺伝子解析用サンプルの製造方法

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Also Published As

Publication number Publication date
FI924242A0 (fi) 1992-09-22
CA2078703A1 (en) 1993-03-24
GR3021721T3 (en) 1997-02-28
DE69214243T2 (de) 1997-02-06
DE69214243D1 (de) 1996-11-07
EP0534640B1 (en) 1996-10-02
FI924242A (fi) 1993-03-24
DK0534640T3 (es) 1997-03-17
CA2078703C (en) 2000-10-17
ATE143700T1 (de) 1996-10-15
FI103413B1 (fi) 1999-06-30
FI103413B (fi) 1999-06-30
JP2703156B2 (ja) 1998-01-26
US5643730A (en) 1997-07-01
JPH05192199A (ja) 1993-08-03
EP0534640A1 (en) 1993-03-31

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