CN1154734C - 突变的5-烯醇丙酮酰莽草酸-3-磷酸合酶以及编码此蛋白的基因 - Google Patents

突变的5-烯醇丙酮酰莽草酸-3-磷酸合酶以及编码此蛋白的基因 Download PDF

Info

Publication number
CN1154734C
CN1154734C CNB961968893A CN96196889A CN1154734C CN 1154734 C CN1154734 C CN 1154734C CN B961968893 A CNB961968893 A CN B961968893A CN 96196889 A CN96196889 A CN 96196889A CN 1154734 C CN1154734 C CN 1154734C
Authority
CN
China
Prior art keywords
ala
val
leu
gly
thr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CNB961968893A
Other languages
English (en)
Other versions
CN1196088A (zh
Inventor
Ъ
米歇尔·利布朗
阿兰·赛兰德
乔治斯·弗赖西耐特
¸
埃里克·德格里西
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer LLC
Bayer CropScience SA
Original Assignee
Rhone Poulenc Agrochimie SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=9481324&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN1154734(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Rhone Poulenc Agrochimie SA filed Critical Rhone Poulenc Agrochimie SA
Publication of CN1196088A publication Critical patent/CN1196088A/zh
Application granted granted Critical
Publication of CN1154734C publication Critical patent/CN1154734C/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Catching Or Destruction (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

本专利公开了一种草甘膦抗性的，至少包括一个102位异亮氨酸置换苏氨酸的5-烯醇丙酮酰莽草酸-3-磷酸合酶(EPSPS)突变基因，以及用于产生抗草甘膦的转化的植物。

Description

突变的5-烯醇丙酮酰莽草酸-3 -磷酸合酶以及编码此蛋白的基因
本发明涉及一种新的5-烯醇丙酮酰莽草酸-3-磷酸合酶(EPSPS)，它对那些在EPSPS活性中作为磷酸烯醇式丙酮酸(PEP)竞争性抑制剂的除草剂表现出增强的抗性。这种抗性更强的EPSP合酶至少含有一个“异亮氨酸对苏氨酸”的置换。本发明也涉及编码这一蛋白的基因，涉及由含此基因的嵌合基因构建体转化的植物细胞，涉及由这些细胞再生的植株以及使用这些转化植株杂交产生的植株。
草甘膦，sulfosate和fosametine是属于膦酰甲基甘氨酸(phosphonomethylglycine)家族的广谱内吸除草剂。它们主要作为5-烯醇丙酮酰莽草酸-3-磷酸合酶(EC 2.5.1.19.)或EPSPS的竞争性抑制剂，竞争PEP(磷酸烯醇式丙酮酸)。把它们施用到植株后，它们在植株内运输并积累到快速生长的部位，特别是茎和根尖，造成损伤直至破坏敏感植株的程度。
质体EPSPS，这些产品的主要靶目标，是芳香族氨基酸生物合成途径的一种酶，它由一个或多个核基因编码并以胞质前体的形式合成，然后输入到质体并以成熟形式积累。
植株对草甘膦及其同族产品的耐受性是通过向它们的基因组稳定引入EPSPS基因获得，基因是植物或细菌来源的，相对于草甘膦抑制该基因产物的特征是突变的或者不同的。鉴于草甘膦的作用方式和使用基因的产物对草甘膦的耐受程度，能够表达该基因翻译产物以在质体中积累到相当的量是十分便利的。
已知例如美国专利4,535,060，通过向植物基因组引入至少携带一个突变从而在酶定位到质体区后使此酶对它的竞争性抑制剂(草甘膦)更具抗性的一个编码EPSPS的基因，可以给与植物对上述类型除草剂的抗性，特别是N-膦酰甲基甘氨酸(N-phosphonomethylglycine)或草甘膦。然而这些技术都需要改进，使这些植物在田间条件下使用时获得更高的可靠性。
在本描述中，“植物”意味着任何能够进行光合作用的分化的多细胞有机体，“植物细胞”意味着来源于植物并能够形成未分化组织例如愈伤组织或分化的组织例如胚或植株部分或种子的任何细胞。
本发明的目的是通过含有耐受膦酰甲基甘氨酸族除草剂的基因的新嵌合基因转化的细胞的再生，产生对此类除草剂具有增强耐受性的转化植物。
本发明的另一个目的是能够给与植物对以EPSPS作为靶的除草剂具增强耐受性的嵌合基因，在转录方向上包括：一个启动子区，任选的一个转运肽区，编码草甘膦耐受性酶基因和3′末端非翻译的聚腺苷酸信号区的序列，特征是相对于其衍生的基因，草甘膦耐受性基因在“aroA”(EPSPS)区含有一个“异亮氨酸对102位苏氨酸”的置换。优选地，它在同一区域另外包括一个“丝氨酸对106位脯氨酸”的置换。这些置换可以引入到或存在于任何来源的EPSPS序列，特别是植物，细菌，藻类或真菌来源的。
可用于转运肽区的本质已知的转运肽可以是植物来源，例如来源于玉米，向日葵，豌豆，烟草或诸如此类。第一和第二个转运肽可以相同，相似或不同。此外，它们可各自含有按照欧洲专利申请EPO 508 909的一个或多个转运肽单位。此特征区的功能是允许成熟和天然的蛋白，特别是上述突变的EPSPS在质体区的最高效率的释放。
依据本发明嵌合基因的启动子区有利地由在植物中天然表达的至少一个基因启动子或启动子片段组成(微管蛋白，内含子，肌动蛋白，组蛋白)。
嵌合基因3′末端非翻译的转录终止信号区可以是任何来源，例如细菌来源，诸如来自胭脂氨酸合酶基因，或植物来源，诸如来自依据欧洲专利申请(欧洲专利申请号633 317)的拟南芥组蛋白H4A748基因。
除了上述基本部分之外，根据发明的嵌合基因可包括至少一个非翻译中介(接头)区，其位于上述不同转录区之间。此中介区可以是任何来源，例如细菌，病毒或植物来源。
分离编码玉米EPSPS的cDNA：
下面叙述获得用作引入两个突变的基质的玉米EPSPS cDNA的不同步骤。下面描述的所有操作以实施例的形式提供，且对应于为达到同一结果从存在的不同方法中作出的一种选择。这种选择对结果的质量无影响，因而本技术领域熟练技术人员可使用任何合适的方法达到相同的结果。大多数DNA片段基因工程方法描述于“分子生物学通用方法(Current Protocols inMolecular Biology)”卷1和卷2，Ausubel F.M.等，Greene PublishingAssociates and Wiley-Interscience(1989)出版(下文中，对描述于此书中方法的参考文献将命名为“ref.CPMB”)。根据此书中描述方法进行的有关DNA的操作主要如下：DNA片段的连接，用Klenow DNA聚合酶和T4DNA聚合酶处理，微量制备或大量制备质粒和噬菌体λDNA，以及分别依据Southern和Northern技术进行DNA和RNA检测。也采用了描述于此书中的其它方法，并且只有对这些方法进行显著修改或附加时才在下面予以描述。
实施例1：
1.获取拟南芥EPSPS片段
a)根据拟南芥EPSPS基因序列(Klee H.J.等(1987)分子及普通遗传学(Mol.Gen.Genet.)，210，437-442)合成的两段20基体寡聚核苷酸，序列分别是：
5′-GCTCTGCTCATGTCTGCTCC-3′
5′-GCCCGCCCTTGACAAAGAAA-3′
这两段寡聚核苷酸分别在公布序列的1523到1543位和1737到1717位，并且方向相反。
b)拟南芥(哥伦比亚变种)总DNA来自Clontech(目录参考号：6970-1)。
c)把50纳克(ng)DNA与每种300ng寡聚核苷酸混合，使用Perkin-Elmer 9600仪器，在供应商建议的标准扩增介质条件下扩增35循环。产生的204-碱基对(bp)片段形成拟南芥EPSPS片段。
2.用BMS玉米细胞系构建cDNA文库
a)将5克过滤的细胞在液氮中研磨，总核酸的提取按照Shure等描述的方法进行并有以下修改：
-裂解缓冲液pH值调至pH9.0；
-用异丙醇沉淀后，以水溶解沉淀并在溶解后调节至2.5M LiCl。℃温育12小时后，在4℃ 30,000g离心15分钟得到沉淀并重新溶解。然后重复LiCl沉淀步骤。重溶的沉淀含有总核酸的RNA组分。
b)按照“分子生物学通用方法(Current Protocols in Molecular Biology)”所述在寡聚脱氧胸腺苷酸-纤维素柱(oligo(dT)-cellulose column)上色谱法获得RNA组分中的聚腺苷酸化RNA(poly(A)+RNA)组分。
c)具有合成的EcoRI末端的双链cDNA的合成：这一步骤按照合成所需的以试剂盒形式提供的各种试剂的供应商提供的方法进行：“复制试剂盒(copy kit)”来自In Vitrogen公司。
将分别具有序列：
5′-AATTCCCGGG-3′
5′-CCCGGG-3′(后者已磷酸化)
的两单链及其部分互补寡聚核苷酸与平头末端的双链cDNA连接。
衔接子的连接导致产生了结合到双链cDNA的SmaI位点和在每一双链cDNA末端粘性形式的EcoRI位点。
d)文库的创建
依照供应商New England Biolabs提供的方法将在其末端含有人工粘性EcoRI位点的cDNA与用EcoRI酶切并去磷酸化的噬菌体λgt10 cDNA相连接。
按照供应商的说明书，使用叫作Gigapack Gold的包装抽提物在体外包装一份连接反应物；用细菌大肠杆菌C600hfl(E.coli C60ohfl)滴定文库。由此获得的文库按照同一供应商的说明加以扩增和贮存，形成BMS玉米细胞悬液cDNA文库。
3.使用拟南芥EPSP探针筛选BMS玉米细胞悬液cDNA文库。
遵循“分子生物学通用方法(Current Protocols in Molecular Biology)”卷1和2，Ausubel F.M.等，Greene Publishing Associates and Wiley-Interscience(1989)(CPMB)中所述的方法。简洁说明，大约106个重组噬菌体以平均密度100个噬菌体/平方厘米在LB平皿中铺平板。裂解噬菌斑在Amersham公司Hybond N膜上双份复制。
通过1600KJ紫外处理(Stratagene Stratalinker)把DNA固定于滤膜。滤膜在65℃下6×SSC/0.1％SDS/0.25脱脂乳中预杂交2小时。根据供应商建议(Pharmacia Ready to Go kit)使用自由引物方法将拟南芥EPSPS探针用[32P]dCTP标记。获得的特异活性具有每微克片段108cpm的数量级。在100℃下变性5分钟后，将探针加入到预杂交介质中并在55℃下继续杂交14小时。使用Kodak XAR5胶片和Amersham Hyperscreen RPN增感屏，将滤膜在-80℃下荧光自显影48小时。对比滤膜上的阳性位点与它们来源的平皿，能够从该平皿中筛选出相应于与拟介芥EPSPS探针显示阳性杂交反应的噬菌体的区域。重复此铺平板，转化，杂交和再生的步骤，直到连续纯化的噬菌体在平板上的全部位点在杂交时证实有100％阳性。然后，在稀释的入培养基中(Tris-Cl pH7.5；10mM MgSO4；0.1M NaCl；0.1％明胶)挑选噬菌体裂解的独立噬菌斑；溶液中的这些噬菌体构成BMS玉米细胞悬液的EPSP-阳性克隆。
4.BMS玉米细胞悬液EPSP克隆DNA的制备和检测
将大约5×108噬菌体加到20毫升OD600nm值为2/毫升的C600hfl菌液中并在37℃下温育15分钟。然后将悬液稀释到一个1升三角瓶中的200毫升细菌生长培养基中，并以250rpm的速度在旋转搅拌器上搅拌。对应于混浊菌液的裂解，在搅拌大约4小时后，培养液澄清指示显著裂解。然后按“分子生物学通用方法(Current Protocols in Molecular Biology)”中所述处理此清液。获得的DNA对应于BMS玉米细胞悬液EPSP克隆。
用EcoRI酶切1到2微克此DNA并在0.8％LGTA/TBE琼脂糖凝胶上分离(参考CPMB)。最终结论是发现纯化的DNA确实与拟南芥EPSPS探针显示杂交信号。电泳之后，按照描述于“分子生物学通用方法(Current Protocolsin Molecular Biology)”中的Southem方法将DNA片段转移到AmershamHybond N膜。按照上述第3部分所述的条件将滤膜与拟南芥EPSPS探针杂交。显示与拟南芥EPSPS探针杂交信号并含有最长EcoRI片段的克隆按凝胶带估计具有大约1.7kbp的大小。
5.获得克隆pRPA-ML-711
用EcoRI消化10微克含有1.7kbp插入片段的噬菌体克隆并在0.8％LGTA/TBE琼脂糖凝胶上分离(参考CPMB)。从BET染色的凝胶上切下含有1.7kbp插入片段的凝胶片段，并按照供应商New England Biolabs的方法用β-琼脂糖酶处理该片段。按照描述于“分子生物学通用方法(Cwrrent Protocolsin Molecular Biology)”中的连接方法将1.7kbp片段纯化DNA与用EcoRI酶切的质粒PUC 19(New England Biolabs)DNA在12℃下连接14小时。两微升上述连接混合物用于转化等份的电感受态大肠杆菌(E.coli)DH10B；以电穿孔法进行转化，使用下面条件：将感受态细菌和连接液混合物引入到一个厚度0.2厘米的电穿孔室(Biorad)，预冷至0℃。使用Biorad制造的电穿孔仪，电穿孔的物理条件是2500伏特，25微法拉和200欧姆。在此条件下，电容器(Condenser)的平均放电时间在4.2毫秒的数量级。然后将细菌溶解于1毫升SOC培养基(ref.CPMB)并于15毫升角状管(coring tubes)中在旋转搅拌器上在200rpm搅拌1小时。按照描述于“分子生物学通用方法”(Current Protocols inMolecular Biology)”中的方法，在补充有100微克/毫升的羧苄青霉素的LB/琼脂培养基上铺平板后，产生了在37℃下生长一夜的微制备的细菌克隆。用EcoRI消化DNA并在0.8％LGTA/TBE琼脂糖凝胶上电泳分离后(ref.CPMB)，保留含有1.7-kbp插入片段的克隆。最终结论是发现纯化的DNA确实显示与拟南芥EPSPS探针的杂交信号。电泳之后，按照描述于“分子生物学通用方法(Current Protocols in Molecular Biology)”中的Southem方法将DNA片段转移到Amersham Hybond N膜。按照描述于上述第3部分的条件将滤膜与拟南芥EPSPS探针杂交。大量制备含有1.7kbp插入片段并与拟南芥EPSPS探针杂交的质粒克隆，并将细菌裂解产生的DNA按“分子生物学通用方法(Current Protocols in Molecular Biology)”中所述用氯化铯梯度纯化。纯化的DNA使用Pharmacia试剂盒按照供应商的说明并用定购于同一供应商M13正向和反向通用引物作为引物进行部分测序。产生的部分序列大约有0.5kbp。衍生的在成熟蛋白区的氨基酸序列(大约50氨基酸残基)与描述于美国专利USP 4,971,908中的成熟玉米EPSPS的相应氨基酸序列显示100％同源性。命名这一相应于BMS玉米细胞悬浮EPSPS DNA的1.7kbp EccRI片段的克隆为pRPA-ML-711。使用Pharmacia试剂盒方法确定了此克隆每链的全序列并且合成互补寡核苷酸及其相反方向寡核苷酸各大约250bp。获得的此1713bp克隆的全序列显示于序列1。
6.获得克隆pRPA-ML-715
克隆pRPA-ML-711序列的检测，特别是将衍生的氨基酸序列与玉米的相比较，显示在编码玉米EPSPS(美国专利USP 4,971,908)成熟部分N末端丙氨酸的GCG密码子上游有一段92bp的序列延伸。相似地，观察到在编码玉米EPSPS(美国专利USP 4,971,908)成熟部分C末端天冬酰胺的AAT密码子下游有一段288bp的延伸。这两个部分可能是，N端延伸对应于用于质体定位的转运肽序列的一部分，C端延伸对应于非翻译的cDNA3′区域。
为了获得编码玉米EPSPS cDNA成熟部分的cDNA，如USP 4,971,908中所述，进行了以下操作：
a)除去非翻译的3′区域：构建pRPA-ML-712：
用限制性酶AseI切克隆pRPA-ML-711，并按照CPMB中所述方法使用DNA聚合酶I的Klenow片段把由此裂解产生的末端处理成钝末端。然后用限制性酶SacII进行切割。由这些操作产生的DNA通过在1％LGTA/TBE琼脂糖凝胶上电泳进行分离(ref.CPMB)。
从凝胶上切下含0.4-kbp“AseI-钝末端/SacII”插入片段的胶片段并按照描述于上述第5部分的方法纯化。用限制性酶HindIII在克隆载体PUC19多接头的HindIII位点切割克隆pRPA-ML-711DNA，并用DNA聚合酶I的Klenow片段把此切割产生的末端处理成钝末端。然后用限制性酶SacII切割处理。从这些操作产生的DNA在0.7％LGTA/TBE琼脂糖凝胶上电泳分离(ref.CPMB)。
从凝胶上切下含有大约3.7kbp HindIII-钝末端/SacII插入片段的胶片段并按照描述于上述第5部分的方法纯化。
将两插入片段连接，并用2微升连接混合物按照上述第5部分所述转化大肠杆菌DH10B。
按照描述于pRDA-ML-711的步骤检测不同克隆的质粒DNA内容。选择的一个质粒克隆含有大约1.45-kbp的EcoRI-HindIII插入片段。这一克隆的末端序列揭示插入片段的5′末端准确对应于pRPA-ML-711的相应末端，并且其3′末端具有下面序列：
“5′-… AATTAAGCTCTAGAGTCGACCTGCAGGCATGCAAGCTT-3′”。
划线序列对应C末端氨基酸天冬酰胺的密码子，下一个密码子对应翻译终止密码子。下游的核苷酸对应PUC 19多接头的序列元素。此克隆包括pRPA-ML-711序列直至成熟玉米EPSPS的翻译终止位点，其后的PUC 19多接头序列直至HindIII位点命名为pRPA-ML-712。
b)pRPA-ML-7125′末端的修饰：
构建pRPA-ML-715：
用限制性酶PstI和HindIII切克隆pRPA-ML-712。将这些操作产生的DNA在0.8％LGTA/TBE琼脂糖凝胶上电泳分离(参照CPMB)。从凝胶上切下含有1.3-kbp PstI-EcoRI插入片段的胶片段并按照上面第5部分所述的方法纯化。此插入片段在等摩尔量的每种下面两个部分互补寡核苷酸序列存在下：
寡核苷酸1：5′-GAGCCGAGCTCCATGGCCGGCGCCGAGGAGATCGTGCTGCA-3′
寡核苷酸2：5′-GCACGATCTCCTCGGCGCCGGCCATGGAGCTCGGCTC-3′以及用限制性酶BamHI和HindIII消化的质粒PUC 19 DNA存在下连接。
使用2微升连接混合物按上面第5部分所述转化大肠杆菌DH10B。按照描述于上面第5部分的步骤对不同克隆的质粒DNA内容检测后，保留一个含有大约1.3-kbp插入片段的克隆用于下面的检测。选择的克隆5′末端序列揭示此区域的DNA序列如下：PUC 19多接头从EcoRI到BamHI位点的序列，其后是用于克隆的寡核苷酸序列，最后是存在于pRPA-ML-712的残余序列。此克隆命名为pRPA-ML-713。此克隆含有一个包括在成熟EPSP合酶N末端丙氨酸密码子上游NcoI位点的甲硫氨酸ATG密码子。此外，保留了N末端丙氨酸和甘氨酸密码子，但修改了第三个可变碱基：初始的GC GGG T修改为GC CGG C。
用限制性酶HindIII切克隆pRPA-ML-713，并用DNA聚合酶IKlenow片段把切割末端处理成钝末端。然后用限制性酶SacI切割。由这些操作产生的DNA在0.8％LGTA/TBE琼脂糖凝胶上电泳分离(ref.CPMB)。从凝胶上切下含有1.3-kbp“HindIII-钝末端/SacI”插入片段的胶片段，并按上面第5部分所述的方法纯化。此插入片段在用限制性酶XbaI消化并用DNA聚合酶I的Klenow片段将切割末端处理成钝末端的质粒PUC 19DNA存在下连接。然后用限制性酶SacI切割。两微升连接混合物如上面第5部分所述用于转化大肠杆菌DH10B。按照上面第5部分所述方法对不同克隆的质粒DNA内容检测之后，保留一个含有大约1.3-kbp插入片段的克隆进一步检测。选择的克隆末端序列揭示DNA序列如下：PUC 19多接头从EcoRI到SacI位点的序列，其后是用于克隆的寡核苷酸序列，其中缺失了上述寡核苷酸1的4bpGATCC，最后是存在于pRPA-ML-712直至HindIII位点的残余序列以及PUC 19多接头从XbaI到HindIII的序列。此克隆命名为pRPA-ML-715。
7.获得编码突变的玉米EPSPS的cDNA
所有的突变步骤都按照供应商说明使用Pharmacia U.S.E突变发生试剂盒进行。此突变发生体系的原理如下：热变性质粒DNA并在一摩尔过量的一方面突变发生寡核苷酸，以及另一方面能够消除存在于多接头的特定限制性酶切位点的寡核苷酸存在下重缔合。重缔合步骤后，在提供的合适缓冲液中T4 DNA连接酶和基因32蛋白存在下利用T4 DNA聚合酶作用合成互补链。合成产物在假定其位点已通过突变除去的限制性酶存在下温育。特别是含有mutS突变的大肠杆菌菌株用作此DNA转化的宿主。在液体培养基中生长后，制备总质粒DNA并在前面使用的限制性酶存在下温育。这些处理之后，使用大肠杆菌菌株DH10B作为转化的宿主。制备分离克隆的质粒DNA，并通过测序证实引入突变的存在。
A)-基本上不影响玉米对作为EPSP合酶活性竞争性抑制剂的产品的EPSPS-抗性特征的位点或序列修饰：从pRPA-ML-715除去一个内部NcoI位点
通过把N末端丙氨酸密码子GCC的第一个碱基置于1位对pRPA-ML-715序列人为编号。此序列在1217位含有一个NcoI位点。该位点修饰的寡核苷酸序列为：
5′-CCACAGGATGGCGATGGCCTTCTCC-3′。
按照上面提供的参考文献测序后，突变后序列阅读对应于所用的寡聚核苷酸。确实已除去了NcoI位点，并且此区域向氨基酸的翻译保留了pRPA-ML-715中存在的初始序列。
此克隆命名为pRPA-ML-716。
此克隆的1340-bp序列存在于序列2和序列3。
B)-使玉米具有对作为EPSP合酶活性竞争性抑制剂的产品的EPSPS-抗性特征增强的序列修饰。
使用下列寡核苷酸：
a)突变苏氨酸(Thr)102→异亮氨酸(Ile)。
5′-GAATGCTGGAATCGCAATGCGGCCATTGACAGC-3′
b)突变脯氨酸(Pro)106→丝氨酸(Ser)。
5′-GAATGCTGGAACTGCAATGCGGTCCTTGACAGC-3′
c)突变甘氨酸(Gly)101→丙氨酸(Ala)和苏氨酸(Thr)102→异亮氨酸(Ile)。
5′-CTTGGGGAATGCTGCCATCGCAATGCGGCCATTG-3′
d)突变苏氨酸(Thr)102→异亮氨酸(Ile)和脯氨酸(Pro)106→丝氨酸(Ser)。
5′-GGGGAATGCTGGAATCGCAATGCGGTCCTTGACAGC-3′
测序表明，三个突变片段突变后的序列阅读除了对应于使用的突变产生寡核苷酸的突变产生区域之外，其余都与母本pRPA-ML-716DNA序列相同。命名这些克隆：pRPA-ML-717对应突变Thr102→Ile，pRPA-ML-718对应突变Pro106→Ser，pRPA-ML-719对应突变Gly101→Ala和Thr102→Ile以及pRPA-ML-720对应突变Thr102→Ile和Pro106→Ser。
pRPA-ML-720的1340-bp序列存在于序列4和序列5。
1395-bp的NcoI-HindIII插入片段是所有构建体的基础，这些构建体用于转化植物以引入对那些作为EPSPS竞争性抑制剂的除草剂的抗性，特别是草甘膦抗性。此插入片段将在后面的描述“玉米EPSPS双突变”中指明。
实施例2：
各种突变体的体外草甘膦耐受性
2.a：提取EPSP合酶
将各种EPSP合酶基因以NcoI-HindIII盒形式引入用NcoI和HindIII酶切的质粒载体pTrc99a(Pharmacia，参照：27-5007-01)。超量表达各种EPSP合酶的重组大肠杆菌DH10B在每10克沉淀细胞40毫升缓冲液中超声波处理，并用加有1克聚乙烯吡咯烷酮的同种缓冲液(200mM Tris-HClpH7.8，50mM巯基乙醇，5mM EDTA和1mM PMSF)洗涤。悬液在4℃下搅拌15分钟，然后在4℃下27,000g离心20分钟。
向上清液中加入硫酸铵使溶液达到40％的硫酸铵饱和度。混合液在4℃下27,000g离心20分钟。将硫酸铵加入新的上清液使溶液达到70％的硫酸铵饱和度。混合液在4℃下27,000g离心30分钟。存在于此蛋白沉淀的EPSP合酶溶解于1毫升缓冲液(20mM Tris-HCl pH7.8和50mM巯基乙醇)。将此溶液对两升同种缓冲液在4℃下透析过夜。
2.b：酶活性
每种酶活性及其草甘膦抗性在下面的反应混合液中于37℃下10分钟内体外测定：100mM马来酸pH5.6，1mM磷酸烯醇式丙酮酸，3mM莽草酸3-磷酸(按照Knowles PF和Sprinson D.B.1970.酶学方法(Methods inEnzymol)17A，351-352制备，来自产气气杆菌(Aerobacter aerogenes)ATCC25597)和10mM氟化钾。酶提取物在加入草甘膦后立即加入，终浓度为0到20mM。
按照Tausky H.A.和Shorr E.1953.生物化学杂志(J.Biol.Chem.)202，675-685中的技术通过测定释放的磷酸盐确定酶活性。
在这种情况下，野生型(WT)酶在草甘膦浓度为0.12mM时已有85％被抑制。在此浓度下，名为Ser106的突变酶只有50％抑制，并且另外三个突变体，Ile102，Ile102/Ser106和Ala101/Ile102表现极小的甚至没有抑制。
为了产生对突变体酶Ile102 50％的抑制，必须把草甘膦浓度增大10倍，即1.2mM，而突变体Ile102/Ser106，Ala/Ile和Ala仍不被抑制。
应当指出的是，直到草甘膦浓度为10mM时突变体Ala/Ile和Ala仍不被抑制，并且甚至草甘膦浓度加倍，即20mM时，突变体Ile102/Ser106活性仍无减少。
实施例3：
转化的烟草植株的抗性
1-1-转化
将载体pRPA-RD-173引入携带粘性pTVK291(Komari等.，1986)的根瘤土壤杆菌(Agrobacterium tumefaciens)菌株EHA101(Hood等.，1987)。转化技术基于Horsh等，(1985)的方法。
1-2-再生
在含有30克/升蔗糖和200微克/毫升卡那霉素的Murashige和Skoog(Ms)基本培养基上从叶外植体(leaf explants)进行PBD6烟草(来自SEITA法国)的再生。叶外植体取自温室培养或体外培养的植株，并按照叶盘法(leaf disctechnique)(科学(Science)，1985，卷227，PP.1229-1231)通过三个连续步骤转化：第一步包括在补充有30克/升蔗糖包含0.05毫克/升萘乙酸(NAA)和2毫克/升苄基氨基嘌呤(BAP)的培养基上诱导苗15天。然后将这一步形成的苗通过在补充有30克/升蔗糖但不含任何激素的MS培养基上培养发育10天。随后把发育的苗移到具有一半浓度盐，维生素和蔗糖且不含任何激素的MS生根培养基上培养。大约15天后，将生根的苗移至土壤。
1-3-草甘膦抗性
二十株转化植株得以再生并转移到温室用以构建pRPA-RD-173。这些植株在温室中5叶期时用总计相应于每公顷0.8公斤的草甘膦活性物质的水悬液处理。
结果表示处理3星期后记录的植物毒性指数。在这种情况下，发现用构建体pRPA-RD-173转化的植株显示良好的耐受性，而未转化的对照植株完全被破坏。
这些结果清楚表明由于使用依据本发明的嵌合基因作为编码草甘膦耐受性的同一基因带来的改进。
实施例4：
转化和选择玉米细胞
依据Klein等.1987年所述的原理和方法(Klein T.M.，Wolf E.D.，Wu R.和Sandford J.C.(1987)：用于将核酸传递至活细胞的高速微投射(High Velocitymicroprojectiles for delivering nucleic acids into living cells)，自然(NATURE)Vol.327 pp.70-73)用构建体pRPA-RD-130轰击对数生长期的BMS(黑墨西哥甜(Black Mexican Sweet))玉米细胞。
轰击两天后，将细胞转移至含有2mM N-(膦酰甲基)甘氨酸的同一培养基。
在此培养基上筛选8星期后，选择发育的愈伤组织，然后用聚合酶链式反应(PCR)扩增并检测，清楚地揭示了嵌合的OTP-EPSPS基因的存在。
未被轰击并在含有2mM N-(膦酰甲基)甘氨酸的相同培养基上生长的细胞被除草剂阻碍而不发育。
依据本发明的转化植株可用作亲本以获得具有对应于引入的嵌合基因表达的表型特征的种系或杂种。
质粒构建体的描述
pRPA-RD-124：将一个“nos”聚腺苷酸化信号添加到pRPA-ML-720，并伴有包括玉米双突变EPSPS基因(Thr102→Ile和Pro106→Ser)的克隆盒的形成。用HindIII消化pRPA-RD-720并用大肠杆菌DNA聚合酶I的Klenow片段处理形成钝末端。用NcoI进行第二次消化，并纯化EPSPS片段。然后将EPSPS基因与纯化的pRPA-RD-12(含有胭脂氨酸合酶聚腺苷酸信号的一个克隆盒)相连接以形成pRPA-RD-124。为了获得有效的纯化载体pRPA-RD-12，必须预先用SalI消化后者，用KlenowDNA聚合酶处理然后用NcoI第二次消化。
pRPA-RD-125：将一个最优化转运肽(OTP)添加到pRPA-RD-124，并伴有包含定向到质粒的EPSPS基因的克隆盒的形成。用SphI消化pRPA-RD-7(欧洲专利申请EP 65 2286)，用T4 DNA聚合酶处理然后用SpeI消化，并纯化OTP片段。将此OTP片段克隆到pRPA-RD-124中，它已预先用NcoI消化，用Klenow DNA聚合酶处理除去突出的3′部分然后用SpeI消化。然后对此克隆测序以保证OTP和EPSPS基因之间正确的翻译融合。这样获得了pRPA-RD-125。
pRPA-RD-130：将H3C4玉米组蛋白启动子和pRPA-RD-123(专利申请EP 507698)的adhl内含子1序列添加到pRPA-RD-125，并伴有用于植物中表达单子叶植物组织中的双突变EPSPS基因的表达盒的形成。用NcoI和SacI消化pRPA-RD-123(一个包含H3C4玉米组蛋白启动子与adhl内含子1融合的盒)。然后纯化含有来自pRPA-RD-123的启动子的DNA片段，并与预先用NcoI和SacI消化的pRPA-RD-125连接。
pRPA-RD-159：将H4A748拟南芥属(Arabidopsis)组蛋白双启动子(专利申请EP 507698)添加到pRPA-RD-125，并伴有用于植物中表达双子叶植物组织中的“OTP-双突变EPSPS基因”的表达盒的形成。用NcoI和SacI消化pRPA-RD-132(一个含有H4A748双启动子的盒(专利申请EP507 698))。然后将纯化的启动子片段克隆到已用EcoI和SacI消化的pRPA-RD-125中。
pRPA-RD-173；将pRPA-RD-159的“H4A748启动子-OTP-双突变EPSPS基因”基因添加到质粒pRPA-BL-150A(欧洲专利申请508 909)，并伴有根瘤土壤杆菌(Agrobacterium tumefaciens)转化载体的形成。用NotI消化pRPA-RD-159并用Klenow聚合酶处理。然后将此片段用SmaI克隆到pRPA-BL-150A。
                    序列表
(1)一般资料：
    (i)申请人：Lebrun，Michel
               Sailland，Alain
               Freyssinet Goorgee
               Deqryse Eric
    (ii)发明名称：突变的5-烯醇丙酮酰莽草酸-3-磷酸合酶，编码
                  比蛋白的基因以及含有此基因的转化的植物
    (iii)序列数目：5
    (iv)通讯地址：
        (A)收信人：
        (B)街道：
        (C)城市：
        (E)国家：法国
        (F)邮编：69263
    (v)计算机可读形式：
        (A)媒介类型：软盘
        (B)计算机：IBM兼容PC
        (C)操作系统：PC-DOS/MS-DOS
        (D)软件：Patent In Release#1.0，#1.25版本
    (vi)当前申请资料：
        (A)申请号：08/945,144
        (B)申请日期：
        (C)分类：
    (viii)律师/代理人资料：
        (A)姓名：
    (ix)电讯资料：
        (A)电话：(33)72-29-26-46
        (B)传真：(33)72-29-28-43
(2)序列资料1：
    (i)序列特征：
        (A)长度：1713碱基对
        (B)类型：核酸
        (C)链型：双链
        (D)拓扑结构：线性
    (ii)分子类型：cDNA
    (vi)原始来源：
        (A)生物：zea mays
        (B)品质：黑墨西哥甜玉米(Black Mexicon Sweet)
        (E)组织类型：愈伤组织
    (vii)直接来源：
        (A)文库：lambda qt10
        (B)克隆：pRPA-ML-711
    (xi)序列描述：序列1：
AATCAATTTC ACACAGGAAA CACCTATCAC CATGATTACG AATTCGGGCC CGGGCGCGTG     60
ATCCGGCGGC GGCAGCGGCG GCGGCGGTGC AGGCGGGTGC CGAGGAGATC GTGCTGCAGC    120
CCATCAAGGA GATCTCCGGC ACCGTCAAGC TGCCGGGGTC CAAGTCGCTT TCCAACCGGA    180
TCCTCCTACT CGCCGCCCTC TCCGAGGGGA CAACAGTCCT TCATAACCTG CTGAACAGTG    240
AGGATGTCCA CTACATGCTC GGGGCCTTGA GGACTCTTGG TCTCTCTGTC GAAGCGGACA    300
AAGCTGCCAA AAGAGCTGTA GTTGTTGGCT GTGGTGGAAA GTTCCCAGTT GAGGATGCTA    360
AAGAGGAAGT GCAGCTCTTC TTGGGGAATG CTGGAACTGC AATGCGCCCA TTCACAGCAG    420
CTCTTACTCC TGCTGGTGGA AATGCAACTT ACGTGCTTGA TGGAGTACCA AGAATGAGGG    480
AGAGACCCAT TGGCGACTTG GTTGTCGGAT TGAAGCAGCT TGGTGCAGAT GTTGATTGTT    540
TCCTTGGCAC TGACTGCCCA CCTGTTCGTG TCAATGGAAT CGGAGGCCTA CCTGGTGGCA    600
AGGTCAAGCT GTCTGGCTCC ATCACCACTC AGTACTTGAG TGCCTTGCTG ATGGCTGCTC    660
CTTTGGCTCT TGGGGATGTG GAGATTGAAA TCATTGATAA ATTAATCTCC ATTCCGTACG    720
TCGAAATGAC ATTGAGATTG ATGGAGCGTT TTGGTGTGAA AGCAGAGCAT TCTGATAGCT    780
GGGACAGATT CTACATTAAG GGAGGTCAAA AATACAACTC CCCTAAAAAT GCCTATGTTG    540
AAGGTGATGC CTCAAGCGCA AGCTATTTCT TGGCTGGTGC TGCAATTACT GGAGGGACTG    900
TGACTGTGGA AGGTTGTGGC ACCACCAGTT TGCAGGGTGA TGTGAAGTTT GCTGAGGTAC    960
TGGAGATGAT GGGAGCGAAC GTTACATGGA CCGAGACTAC CCTAACTCTT ACTGGCCCAC   1020
CGCGGGAGCC ATTTGGGAGG AAACACCTCA AGGCGATTGA TGTCAACATG AACAAGATGC   1080
CTGATGTCGC CATGACTCTT GCTGTGGTTG CCCTCTTTGC CGATGGCCCC ACAGCCATCA   1140
GAGACGTGGC TTCCTGGAGA GTAAAGGAGA CCCACACCAT GGTTGCGATC CCCACCCACC   1200
TAACCAACCT CCCACCATCT GTTGAGGAAG GGCCGGACTA CTGCATCATC ACGCCGCCGG    1260
AGAAGCTGAA CGTGACGGCG ATCGACACGT ACGACGACCA CAGGATGGCC ATGGCCTTCT    1320
CCCTTGCCGC CTGTGCCGAG GTCCCCGTCA CCATCCGGGA CCCTGGGTGC ACCCGGAAGA    1380
CCTTCCCCGA CTACTTCCAT GTGCTGACCA CTTTCGTCAA GAATTAATAA AGCGTGCGAT    1440
ACTACCACGC AGCTTGATTG AAGTGATAGG CTTGTGCTGA GGAAAACATC TTCTTTTGTT    1500
CTGTTTTTCT CTTTCACGGG ATTAAGTTTT GAGTCTGTAA CGTTAGTTGT TTGTAGCAAG    1560
TTTCTATTTC GGATCTTAAG TTTCTGCACT GTAAGCCAAA TTTCATTTCA AGAGTGGTTC    1620
CTTCCAATAA TAAGAATAAT AAATTACGTT TCAGTGAAAA AAAAAAAAAA AAAAAAAAAA    1680
AAAAAAAAAA AAAAAAAAAA AACCCGGGAA TTC                                 1713
(2)序列资料2：
    (i)序列特征：
        (A)长度：1340碱基对
        (B)类型：核酸
        (C)链型：双链
        (D)拓扑结构：线性
    (ii)分子类型cDNA
    (vi)原始来源：
        (A)生物：zea mays
        (B)品系：黑墨西哥甜玉米(Black Mexicon Sweet)
    (vii)直接来源：
        (B)克隆：pRPA-ML-716
    (ix)特征：
        (A)名字/关键词：CDS
        (B)位置：6..1337
    (xi)序列描述：序列2：
CCATG GCC GGC GCC GAG GAG ATC GTG CTG CAG CCC ATC AAG GAG ATC      47
      Ala Gly Ala Clu Clu Ilc Val Leu Gln Pro Ile Lys Glu Ile
        1               5                  10
TCC GCC ACC GTC AAG CTG CCC CCC TCC AAG TCG CTT TCC AAC CGG ATC    95
Ser Gly Thr Val Lys Leu Pro Gly Ser Tys Ser Leu Ser Asn Arg Ile
 15                  20                  25                  30
CTC CTA CTC GCC GCC CTG TCC GAG GGG ACA ACA GTG GTT GAT AAC CTG   143
Leu Leu Leu Ala Ala Leu Ser Glu Gly Thr Thr Val Val Asp Asn Leu
                 35                  40                  45
CTG AAC AGT GAG GAT GTC CAC TAC ATG CTC GGG GCC TTG AGG ACT CTT    191
Leu Asn Ser Glu Asp Val His Tyr Met Leu Gly Ala Leu Arg Thr Leu
             50                  55                  60
GGT CTC TCT GTC GAA GCG GAC AAA GCT GCC AAA AGA GCT CTA CTT GTT    239
Gly Leu Ser Val Glu Ala Asp Lys Ala Ala Lys Arg Ala Val Val Val
         65                  70                  75
GGC TGT GGT GGA AAG TTC CCA GTT GAG GAT GCT AAA GAG GAA GTG CAG    287
Gly Cys Gly Gly Lys Pho Pro Val Glu Asp Ala Lys Glu Glu Val Gln
     80                  85                  90
CTC TTC TTC GGG AAT GCT CCA ACT CCA ATG CGG CCA TTG ACA GCA GCT    335
Leu Phe Leu Gly Asn Ala Gly Thr Ala Met Arg Pro Leu Thr Ala Ala
 95                 100                 105                 110
GTT ACT GCT GCT GGT GGA AAT GCA ACT TAC GTG CTT GAT GGA GTA CCA    383
Val Thr Ala Ala Gly Gly Asn Ala Thr Tyr Val Leu Asp Cly Val Pro
                115                 120                 125
AGA ATG AGG GAG AGA CCC ATT GGC GAC TTG GTT GTC CCA TTC AAC CAC    431
Arg Met Arg Glu Arg Pro Ile Gly Asp Leu Val Val Gly Leu Lys Gln
            130                 135                 140
CTT GGT GCA GAT GTT GAT TGT TTC CTT GGC ACT GAC TGC CCA CCT GTT    479
Leu Gly Ala Asp Val Asp Cys Phe Leu Gly Thr Asp Cys Pro Pro Val
        145                 150                 155
CGT GTC AAT GGA ATC GGA GGG CTA CCT GGT GGC AAG GTC AAG CTG TCT    527
Arg Val Asn Gly Ile Gly Gly Leu Pro Gly Cly Lys Val Lys Leu Ser
    160                 165                 170
GGC TCC ATC AGC AGT CAG TAC TTG AGT GCC TTG CTG ATG GCT GCT CCT    575
Gly Ser Ile Ser Ser Gln Tyr Leu Ser Ala Leu Leu Met Ala Ala Pro
175                 180                 105                 190
TTG GCT CTT GGG GAT GTG GAG ATT GAA ATC ATT GAT AAA TTA ATC TCC    623
Leu Ala Leu Gly Asp Val Glu Ile Glu Ile Ile Asp Lys Leu Ile Ser
                195                 200                 205
ATT CCG TAC GTC GAA ATC ACA TTG AGA TTG ATG GAC CCT TTT CCT CTG    671
Ile Pro Tyr Val Glu Met Thr Leu Arg Leu Met Glu Arg Phe Gly Val
            210                 215                 220
AAA GCA GAG CAT TCT GAT AGC TGG GAC AGA TTC TAC ATT AAG GGA GGT    719
Lys Ala Glu His Ser Asp Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly
        225                 230                 235
CAA AAA TAC AAG TCC CCT AAA AAT GCC TAT GTT GAA GGT GAT GCC TCA    767
Gln Lys Tyr Lys Ser Pro Lys Asn Ala Tyr Val Glu Gly Asp Ala Ser
    240                 245                 250
AGC GCA AGC TAT TTC TTG GCT GGT GCT GCA ATT ACT CGA GGG ACT GTG    815
Ser Ala Ser Tyr Phe Leu Ala Gly Ala Ala Ile Thr Gly Gly Thr Val
355                 260                 265                 270
ACT GTG GAA GGT TGT GGC ACC ACC AGT TTG CAG GGT GAT GTG AAG TTT    863
Thr Val Glu Gly Cye Cly Thr Thr Ser Leu Gln Gly Asp Val Lys Phe
                275                 280                 285
GCT GAG GTA CTG GAG ATG ATG CCA CCC AAC CTT ACA TGG ACC GAG ACT    911
Ala Glu Val Leu Glu Met Met Gly Ala Lys Val Thr Trp Thr Glu Thr
            290                 295                 300
AGC GTA ACT GTT ACT GGC CCA CCG CGG GAG CCA TTT GGG AGG AAA CAC     959
Ser Val Thr Val Thr Gly Pro Pro Arg Glu Pro Phe Gly Arg Lys His
        305                 310                 315
CTC AAG GCG ATT GAT GTC AAC ATG AAC AAG ATG CCT GAT GTC GCC ATG    1007
Leu Lys Ala Ile Asp Val Asn Met Asn Lys Met Pro Asp Val Ala Met
    320                 325                 330
ACT CTT GCT GTG GTT GCC CTC TTT GCC GAT GGC CCG ACA GCC ATC ACA    1055
Thr Leu Ala Val Val Ala Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg
335                 340                 345                 350
GAC GTG GCT TCC TGG AGA GTA AAG GAG ACC GAG AGG ATG GTT GCG ATC    1103
Asp Val Ala Ser Trp Arg Val Lys Glu Thr Glu Arg Met Val Ala Ile
                355                 360                 365
CGG ACG GAG CTA ACC AAG CTG GGA GCA TCT GTT GAG GAA GGG CCG GAC    1151
Arg Thr Glu Leu Thr Lys Leu Gly Ala Ser Val Glu Glu Gly Pro Asp
            370                 375                 380
TAC TGC ATC ATC ACG CCG CCG GAG AAG CTG AAC GTG ACG GCG ATC GAC    1199
Tyr Cys Ile Ile Thr Pro Pro Glu Lys Leu Asn Val Thr Ala Ile Asp
        385                 390                 395
ACG TAC GAC GAC CAC AGG ATG GCC ATG GCC TTC TCC CTT GCC GCC TGT    1247
Thr Tyr Asp Asp His Arg Met Ala Met Ala Pho Ser Leu Ala Ala Cys
    400                 405                 410
GCC GAG GTC CCC CTC ACC ATC CGG GAC CCT CGG TCC ACC CCC AAC ACC    1290
Ala Glu Val Pro Val Thr Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr
 415                420                 425                 430
TTC CCC GAC TAC TTC GAT GTG CTG AGC ACT TTC GTC AAG AAT            1337
Phe Pro Asp Tyr Phe Asp Val Leu Ser Thr Phe Val Lys Asn
                435                 440
TAA                                                                1340
(2)序列资料3：
    (i)序列特征：
        (A)长度：444氨基酸
        (B)类型：氨基酸
        (D)拓扑结构：线性
    (ii)分子类型：蛋白质
    (ix)序列描述：序列3：
Ala Gly Ala Glu Glu Ile Val Leu Gln Pro Ile Lys Glu Ile Ser Gly
  1               5                  10                  15
Thr Val Lys Leu Pro Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu
             20                  25                  20
Lcu Ala Ala Leu Ser Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn
         35                  40                  45
Ser Glu Asp Val His Tyr Met Leu Gly Ala Leu Arg Thr Leu Gly Leu
     50                  55                  60
Ser Val Glu Ala Asp Lys Ala Ala Lys Arg Ala Val Val Val Gly Cys
 65                  70                  75                  80
Gly Gly Lys Phe Pro Val Glu Asp Ala Lys Clu Clu Val Gln Leu Phe
                 85                  90                  95
Leu Gly Asn Ala Gly Thr Ala Met Arg Pro Leu Thr Ala Ala Val Thr
            100                 105                 110
Ala Ala Gly Gly Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met
        115                 120                 125
Arg Glu Arg Pro Ile Gly Asp Leu Val Val Gly Leu Lys Gln Leu Gly
    130                 135                 140
Ala Asp Val Asp Cys Phe Leu Cly Thr Asp Cys Pro Pro Val Arg Val
145                 150                 155                 160
Asn Gly Ile Gly Gly Leu Pro Gly Gly Lys Val Lys Leu Ser Gly Ser
                165                 170                 175
Ile Ser Ser Gln Tyr Leu Ser Ala Leu Leu Met Ala Ala Pro Leu Ala
            180                 185                 190
Leu Gly Asp Val Glu Ile Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro
        195                 200                 205
Tyr Val Glu Met Thr Leu Arg Leu Met Glu Arg Phe Gly Val Lys Ala
    210                 215                 220
Glu His Ser Asp Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Gln Lys
225                 230                 235                 240
Tyr Lys Ser Pro Lys Asn Ala Tyr Val Glu Gly Asp Ala Ser Ser Ala
                245                 250                 255
Ser Tyr Phe Leu Ala Gly Ala Ala Ile Thr Gly Gly Thr Val Thr Val
            260                 265                 270
Glu Gly Cys Gly Thr Thr Ser Leu Gln Gly Asp Val Lys Phe Ala Glu
        275                 280                 285
Val Leu Glu Met Met Gly Ala Lys Val Thr Trp Thr Glu Thr Ser Val
    290                 295                 300
Thr Val Thr Gly Pro Pro Arg Glu Pro Phe Gly Arg Lys His Leu Lys
305                 310                 315                 320
Ala Ile Asp Val Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu
                335                 330                 335
Ala Val Val Ala Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val
            340                 345                 350
Ala Ser Trp Arg Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr
        355                 360                 365
Glu Leu Thr Lys Leu Gly Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys
    370                 375                 380
Ile Ile Thr Pro Pro Glu Lys Leu Asn Val Thr Ala Ile Asp Thr Tyr
385                 390                 395                 400
Asp Asp His Arg Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu
                405                 410                 415
Val Pro Val Thr Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro
            420                 425                 430
Asp Tyr Phe Asp Val Leu Ser Thr Phe Val Lys Asn
        435                 440
(2)序列资料4：
    (i)序列特征：
        (A)长度：1340碱基对
        (B)类型：核酸
        (C)链型：双链
        (D)拓扑结构：线性
    (ii)分子类型：cDNA
    (vi)原始来源：
        (A)生物：zea mays
        (B)品系：黑墨西哥甜玉米(Black Mexicon Sweet)
    (vii)直接来源：
        (B)克隆：pRPA-ML-720
    (ix)特征：
        (A)名字/关键词：CDS
        (B)位置：6..1337
    (xi)序列描述：序列4：
CCATG GCC GGC GCC CAG GAG ATC GTG CTG CAC CCC ATC AAG GAG ATC      47
      Ala Gly Ala Glu Glu Ile Val Leu Gln Pro Ile Lys Glu Ile
        1               5                  10
TCC GGC ACC GTC AAG CTG CCG GGG TCC AAG TCG CTT TCC AAC CGG ATC    95
Ser Gly Thr Val Lys Leu Pro Gly Ser Lys Ser Leu Ser Asn Arg Ile
 15                  20                  25                  30
CTC CTA CTC GCC GCC CTG TCC GAG GGG ACA ACA GTG GTT GAT AAC CTG    143
Leu Leu Leu Ala Ala Leu Ser Glu Gly Thr Thr Val Val Asp Acn Lcu
                 35                  40                  45
CTG AAC AGT GAG GAT GTC CAC TAC ATG CTC GGG GCC TTG AGG ACT CTT    191
Leu Asn Ser Glu Asp Val His Tyr Met Leu Gly Ala Leu Arg Thr Leu
             50                  55                  60
GGT CTC TCT GTC GAA GCG GAC AAA GCT GCC AAA AGA GCT GTA GTT GTT    239
Gly Leu Ser Val Glu Ala Asp Lys Ala Ala Lys Arg Ala Val Val Val
         65                  70                  75
GGC TGT GGT GGA AAG TTC CCA GTT GAG GAT CCT AAA GAG GAA GTG CAG    257
Gly Cys Gly Gly Lys Phe Pro Val Glu Asp Ala Lys Glu Glu Val Gln
     80                  85                  90
CTC TTC TTG GGG AAT GCT GGA ATC GCA ATG CGG TCC TTG ACA GCA GCT    335
Leu Phe Leu Gly Asn Ala Gly Ile Ala Met Arg Ser Leu Thr Ala Ala
 95                 100                 105                 110
GTT ACT GCT GCT GGT GGA AAT GCA ACT TAC GTG CTT GAT GGA GTA CCA    383
Val Thr Ala Ala Gly Gly Asn Ala Thr Tyr Val Lcu Asp Cly Val Pro
                115                 120                 125
AGA ATG AGG GAG AGA CCC ATT GGC GAC TTG GTT GTC GGA TTG AAG CAC    431
Arg Met Arg Glu Arg Pro Ile Gly Asp Leu Val Val Gly Leu Lys Gln
            130                 135                 140
CTT GGT GCA GAT GTT GAT TGT TTC CTT GGC ACT GAC TGC CCA CCT GTT    479
Leu Cly Ala Asp Val Asp Cys Phe Leu Gly Thr Asp Cys Pro Pro Val
        145                 150                 155
CCT CTC AAT CCA ATC CGA GGG CTA CCT GGT GGC AAG GTC AAG CTG TCT    527
Arg Val Asn Gly Ile Gly Gly Leu Pro Gly Gly Lys Val Lys Leu Ser
    160                 165                 170
GGC TCC ATC AGC AGT CAG TAC TTG AGT GCC TTG CTG ATG GCT GCT CCT    575
Gly Ser Ile Ser Ser Gln Tyr Leu Ser Ala Leu Leu Met Ala Ala Pro
175                 180                 185                 190
TTG GCT CTT GGG GAT GTG GAG ATT GAA ATC ATT GAT AAA TTA ATC TCC    623
Leu Ala Leu Gly Asp Val Glu Ile Glu Ile Ile Asp Lys Leu Ile Ser
                195                 200                 205
ATT CCG TAC GTC GAA ATG ACA TTG AGA TTG ATG GAG CGT TTT GGT GTC    671
Ile Pro Tyr Val Glu Met Thr Leu Arg Leu Met Glu Arg Phe Gly Val
            210                 215                 220
AAA GCA GAG CAT TCT GAT AGC TGG GAC AGA TTC TAC ATT AAG GGA GGT    719
Lyo Ala Clu His Ser Asp Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly
        225                 230                 235
CAA AAA TAC AAC TCC CCT AAA AAT GCC TAT GTT GAA GGT GAT GCC TCA    767
Gln Lys Tyr Lys Ser Pro Lys Asp Ala Tyr Val Glu Gly Asp Ala Ser
    240                 245                 250
AGC GCA AGC TAT TTC TTG GCT GGT GCT GCA ATT ACT GGA GGG ACT GTG    815
Ser Ala Ser Tyr Phe Leu Ala Gly Als Ala Ile Thr Gly Gly Thr Val
255                 260                 265                 270
ACT GTG GAA GGT TGT GGC ACC ACC AGT TTG CAG GGT GAT GTG AAG TTT    863
Thr Val Glu Gly Cys Gly Thr Thr Ser Leu Cln Cly Asp Val Lys Phe
                275                 280                 285
GCT GAG GTA CTG GAG ATG ATG CCA CCG AAG GTT ACA TGG ACC CAC ACT    911
Ala Glu Val Leu Glu Met Met Gly Ala Lys Val Thr Trp Thr Glu Thr
            290                 295                 300
AGC GTA ACT GTT ACT GGC CCA CCG CGG GAG CCA TTT GGG AGG AAA CAC    959
Ser Val Thr Val Thr Gly Pro Pro Arg Glu Pro Phe Gly Arg Lys His
        305                 310                 315
CTC AAG GCG ATT GAT CTC AAC ATG AAC AAG ATG CCT GAT GTC GCC ATG    1007
Leu Lys Ala Ile Asp Val Asn Met Asn Lys Met Pro Asp Val Ala Met
    320                 325                 330
ACT CTT CCT CTG GTT GCC CTC TTT GCC GAT CCC CCC ACA CCC ATC AGA    1055
Thr Leu Ala Val Val Ala Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg
335                 340                 345                 350
GAC GTG GCT TCC TGG AGA GTA AAG GAG ACC GAG AGG ATG GTT GCG ATC    1103
Asp Val Ala Ser Trp Arg Val Lys Glu Thr Glu Arg Met Val Ala Ile
                355                 360                 365
CGG ACG GAG CTA ACC AAG CTG GGA GCA TCT GTT GAG GAA GGG CCG GAC    1151
Arg Thr Glu Leu Thr Lys Leu Gly Ala Ser Val Glu Glu Gly Pro Asp
            370                 375                 380
TAC TGC ATC ATC ACG CCG CCG GAG AAG CTG AAC GTG ACG GCG ATC GAC    1199
Tyr Cys Ile Ile Thr Pro Pro Glu Lys Leu Asn Val Thr Ala Ile Asp
        395                 390                 395
ACG TAC GAC GAC CAC AGG ATG GCG ATG GCC TTC TCC CTT GCC GCC TGT    1247
Thr Tyr Asp Asp Hic Arg Met Ala Met Ala Phe Ser Leu Ala Ala Cys
    400                 405                 410
GCC GAG GTC CCC GTC ACC ATC CCC CAC CCT CCC TCC ACC CGG AAG ACC    1295
Ala Glu Val Pro Val Thr Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr
415                 420                 425                 430
TTC CCC GAC TAC TTC GAT GTG CTG AGC ACT TTC GTC AAG AAT            1337
Phe Pro Asp Tyr Phe Asp Val Leu Ser Thr Phe Val Lys Asn
                435                 440
TAA                                                                1340
(2)序列资料5：
    (i)序列特征：
        (A)长度：444氨基酸
        (B)类型：氨基酸
        (D)拓引结构：线性
    (ii)分子类型：蛋白质
    (ix)序列描述：序列5：
Ala Gly Ala Glu Glu Ile Val Leu Gln Pro Ile Lys Glu Ile Ser Gly
  1               5                  10                  15
Thr Val Lys Leu Pro Gly Ser Lys Ser Leu Ser Asn Arg Ile Leu Leu
             20                  35                  30
Leu Ala Ala Leu Ser Glu Gly Thr Thr Val Val Asp Asn Leu Leu Asn
         35                  40                  45
Scr Glu Asp Val His Tyr Met Leu Gly Ala Leu Arg Thr Leu Gly Leu
     50                  55                  60
Ser Val Clu Ala Asp Lys Ala Ala Lys Arg Ala Val Val Val Gly Cys
 65                  70                  75                  80
Gly Gly Lys Phe Pro Val Glu Asp Ala Lys Glu Glu Val Gln Leu Phe
                 85                  90                  95
Leu Gly Asn Ala Cly Ile Ala Met Arg Ser Leu Thr Ala Ala Val Thr
            100                 105                 110
Ala Ala Gly Gly Asn Ala Thr Tyr Val Leu Asp Gly Val Pro Arg Met
        115                 120                 125
Arg Glu Arg Pro Ilc Cly Asp Leu Val Val Gly Leu Lys Gln Leu Gly
    130                 135                 140
Ala Asp Val Asp Cys Phe Lcu Gly Thr Asp Cys Pro Pro Val Arg Val
145                 150                 155                 160
Acn Gly Ile Gly Gly Leu Pro Cly Cly Lys Val Lys Leu Ser Gly Ser
                165                 170                 175
Ile Ser Ser Gln Tyr Leu Ser Ala Leu Lcu Met Ala Ala Pro Leu Ala
            180                 185                 190
Leu Gly Asp Val Glu Ile Glu Ile Ile Asp Lys Leu Ile Ser Ile Pro
        195                 200                 205
Tyr Val Glu Met Thr Leu Arg Leu Met Glu Arg Phe Cly Val Lys Ala
    210                 215                 220
Glu His Ser Asp Ser Trp Asp Arg Phe Tyr Ile Lys Gly Gly Cln Lys
225                 230                 235                 240
Tyr Lys Ser Pro Lys Asn Ala Tyr Val Glu Cly Asp Ala Scr Scr Ala
                245                 250                 255
Ser Tyr Phe Leu Ala Gly Ala Ala Ile Thr Gly Cly Thr Val Thr Val
            260                 265                 270
Glu Gly Cys Gly Thr Thr Ser Leu Gln Gly Asp Val Lye Phe Ala Glu
        275                 280                 285
Val Leu Glu Met Met Gly Ala Lys Val Thr Trp Thr Glu Thr Ser Val
    290                 295                 300
Thr Val Thr Gly Pro Pro Arg Glu Pro Phe Gly Arg Lys His Leu Lye
305                 310                 315                 320
Ala Ile Asp Val Asn Met Asn Lys Met Pro Asp Val Ala Met Thr Leu
                325                 330                 335
Ala Val Val Ala Leu Phe Ala Asp Gly Pro Thr Ala Ile Arg Asp Val
            340                 345                 350
Ala Ser Trp Arg Val Lys Glu Thr Glu Arg Met Val Ala Ile Arg Thr
        355                 360                 365
Glu Leu Thr Lys Leu Gly Ala Ser Val Glu Glu Gly Pro Asp Tyr Cys
    370                 375                 380
Ile Ile Thr Pro Pro Glu Lys Leu Asn Val Thr Ala Ile Asp Thr Tyr
385                 390                 395                 400
Asp Asp His Arg Met Ala Met Ala Phe Ser Leu Ala Ala Cys Ala Glu
                405                 410                 415
Val Pro Val Thr Ile Arg Asp Pro Gly Cys Thr Arg Lys Thr Phe Pro
            420                 425                 430
Asp Tyr Phe Asp Val Leu Ser Thr Phe Val Lys Asn
        435                 440

Claims (12)

1.编码突变的5-烯醇丙酮酰莽草酸-3-磷酸合酶的DNA基因，特征是，该DNA基因至少包括，相对于玉米5-烯醇丙酮酰莽草酸-3-磷酸合酶的氨基酸序列而言，一个异亮氨酸对102位苏氨酸的置换和一个丝氨酸对106位脯氨酸的置换。
2.根据权利要求1所述的DNA基因，特征是它是玉米来源。
3.突变的5-烯醇丙酮酰莽草酸-3-磷酸合酶蛋白，特征是它由权利要求1所述的DNA基因编码获得。
4.在5’和3’位置含有一个编码序列和调节元件的、异源的并能在植物中起作用的嵌合基因，特征是它至少包括权利要求1的序列作为编码序列。
5.根据权利要求4所述的嵌合基因，特征是它含有一个植物病毒启动子。
6.根据权利要求4所述的嵌合基因，特征是它含有一个植物启动子。
7.根据权利要求6所述的嵌合基因，特征是所述植物启动子选自α-微管蛋白、组蛋白、内含子和肌动蛋白的启动子。
8.用于转化植物的载体，特征是它至少含有根据权利要求4所述的嵌合基因。
9.植物细胞，特征是它至少含有根据权利要求4所述的嵌合基因。
10.对以5-烯醇丙酮酰莽草酸-3-磷酸合酶为靶的除草剂具有改进耐受性的植株产生的方法，特征是用根据权利要求1所述基因转化植物细胞或原生质体，并且转化的细胞用于再生。
11.用以5-烯醇丙酮酰莽草酸-3-磷酸合酶为靶的除草剂处理植株的方法，特征是将除草剂施加于根据权利要求9所述的植物细胞再生获得的植株。
12.根据权利要求11所述的方法，特征是使用草甘膦或一种草甘膦前体。
CNB961968893A 1995-07-19 1996-07-18 突变的5-烯醇丙酮酰莽草酸-3-磷酸合酶以及编码此蛋白的基因 Expired - Lifetime CN1154734C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9508979A FR2736926B1 (fr) 1995-07-19 1995-07-19 5-enol pyruvylshikimate-3-phosphate synthase mutee, gene codant pour cette proteine et plantes transformees contenant ce gene
FR95/08979 1995-07-19

Publications (2)

Publication Number Publication Date
CN1196088A CN1196088A (zh) 1998-10-14
CN1154734C true CN1154734C (zh) 2004-06-23

Family

ID=9481324

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB961968893A Expired - Lifetime CN1154734C (zh) 1995-07-19 1996-07-18 突变的5-烯醇丙酮酰莽草酸-3-磷酸合酶以及编码此蛋白的基因

Country Status (31)

Country Link
US (1) US6566587B1 (zh)
EP (2) EP0837944B1 (zh)
JP (2) JP4691733B2 (zh)
KR (1) KR19990029084A (zh)
CN (1) CN1154734C (zh)
AP (1) AP886A (zh)
AT (2) ATE321866T1 (zh)
AU (1) AU6619196A (zh)
BG (1) BG64628B1 (zh)
BR (1) BR9609792A (zh)
CA (1) CA2223875C (zh)
CU (1) CU23172A3 (zh)
CZ (1) CZ295649B6 (zh)
DE (2) DE69635913T2 (zh)
DK (2) DK0837944T3 (zh)
EA (1) EA199800138A1 (zh)
ES (2) ES2255534T3 (zh)
FR (1) FR2736926B1 (zh)
HU (2) HU226302B1 (zh)
IL (1) IL122941A0 (zh)
MX (1) MX9800562A (zh)
NZ (1) NZ313667A (zh)
OA (1) OA10788A (zh)
PL (1) PL189453B1 (zh)
PT (2) PT1217073E (zh)
RO (1) RO120849B1 (zh)
SI (2) SI1217073T1 (zh)
SK (1) SK285144B6 (zh)
TR (1) TR199800065T1 (zh)
UA (2) UA75315C2 (zh)
WO (1) WO1997004103A2 (zh)

Families Citing this family (767)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW305860B (zh) * 1994-03-15 1997-05-21 Toray Industries
DK0975778T3 (da) 1997-04-03 2007-10-08 Dekalb Genetics Corp Anvendelse af glyphostat-resistende majslinier
US6040497A (en) * 1997-04-03 2000-03-21 Dekalb Genetics Corporation Glyphosate resistant maize lines
GB9711015D0 (en) * 1997-05-28 1997-07-23 Zeneca Ltd Improvements in or relating to organic compounds
US6187994B1 (en) 1997-11-18 2001-02-13 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
AU1526199A (en) 1997-11-18 1999-06-07 Pioneer Hi-Bred International, Inc. Targeted manipulation of herbicide-resistance genes in plants
US7102055B1 (en) 1997-11-18 2006-09-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
FR2772787B1 (fr) * 1997-12-24 2001-12-07 Rhone Poulenc Agrochimie Promoteur h3c4 de mais associe au premier intron de l'actine de riz, gene chimere le comprenant et plante transformee
US6153811A (en) 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
CA2332700A1 (en) * 1998-07-10 2000-01-20 Calgene Llc Expression of herbicide tolerance genes in plant plastids
US6492578B1 (en) * 1998-07-10 2002-12-10 Calgene Llc Expression of herbicide tolerance genes in plant plastids
EP1173581A1 (en) * 1999-04-29 2002-01-23 Syngenta Limited Herbicide resistant plants
MXPA01010930A (es) * 1999-04-29 2003-06-30 Syngenta Ltd Plantas resistentes a herbicidas.
AU4134000A (en) 1999-04-29 2000-11-17 Syngenta Limited Herbicide resistant plants
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6271360B1 (en) 1999-08-27 2001-08-07 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
AR025996A1 (es) 1999-10-07 2002-12-26 Valigen Us Inc Plantas no transgenicas resistentes a los herbicidas.
ATE298364T1 (de) * 2000-03-09 2005-07-15 Monsanto Technology Llc Verfahren zum herstellen von glyphosat-toleranten pflanzen
US6580019B1 (en) 2000-03-09 2003-06-17 Dekalb Genetics Corporation Non-reciprocal recombination-mediated transgene deletion in transgenic plants
EP1582583A3 (en) * 2000-03-09 2005-10-12 Monsanto Technology LLP Methods for making plants tolerant to glyphosate and compositions thereof
US6750379B2 (en) 2000-03-09 2004-06-15 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
EP1325136A1 (en) * 2000-09-29 2003-07-09 Syngenta Limited Herbicide resistant plants
US7560622B2 (en) 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
US7462481B2 (en) 2000-10-30 2008-12-09 Verdia, Inc. Glyphosate N-acetyltransferase (GAT) genes
FR2815969B1 (fr) 2000-10-30 2004-12-10 Aventis Cropscience Sa Plantes tolerantes aux herbicides par contournement de voie metabolique
EP1988099B1 (en) 2001-01-09 2012-11-14 Bayer CropScience NV Bacillus thuringiensis insecticidal proteins
AU2002249270B2 (en) 2001-02-22 2006-07-06 Biogemma S.A.S. Constitutive promoter from arabidopsis
US20050081258A1 (en) * 2002-01-18 2005-04-14 Prokopishyn Nicole Lesley Short fragment homologous recombination to effect targeted genetic alterations in plants
CN1330762C (zh) * 2002-05-10 2007-08-08 北京大学 新的草甘膦耐受型5－烯醇丙酮酰莽草酸－3－磷酸合酶及其编码基因
BRPI0311324B1 (pt) 2002-05-22 2020-01-28 Monsanto Technology Llc polinucleotídeo, vetor, polipeptídeo com atividade dessasturase, métodos para produção de planta e óleo de milho contendo ácidos graxos de ômega-3, para aumentar o valor nutricional de um produto comestível, de fabricação de alimento ou alimentação, bem como composição comestível
US7045684B1 (en) 2002-08-19 2006-05-16 Mertec, Llc Glyphosate-resistant plants
FR2844142B1 (fr) 2002-09-11 2007-08-17 Bayer Cropscience Sa Plantes transformees a biosynthese de prenylquinones amelioree
FR2848064B1 (fr) * 2002-12-06 2006-01-13 Bayer Cropscience Sa Plantes legumineuses transplastomiques fertiles
FR2848570B1 (fr) * 2002-12-12 2005-04-01 Bayer Cropscience Sa Cassette d'expression codant pour une 5-enol pyruvylshikimate-3-phosphate synthase (epsps) et plantes tolerantes aux herbicides la contenant
EP1594961B1 (en) * 2003-02-18 2013-12-25 Monsanto Technology LLC Glyphosate resistant class i 5-enolpyruvylshikimate-3-phosphate synthase (epsps)
EP2535414B1 (en) 2003-04-29 2017-12-13 Pioneer Hi-Bred International Inc. Novel glyphosate-n-acetyltransferase (gat) genes
CA2553759A1 (en) * 2004-01-21 2005-08-11 Omega Genetics, Llc Glyphosate tolerant plants and methods of making and using the same
US7405074B2 (en) 2004-04-29 2008-07-29 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GAT) genes
BRPI0518249A2 (pt) * 2004-10-29 2008-11-11 Bayer Bioscience Nv plantas de algodço tolerantes À tensço
US7737326B2 (en) * 2005-04-29 2010-06-15 Midwest Oil Seeds Inc. EPSPS promoter from maize
EP1922409B1 (en) * 2005-08-08 2017-11-08 Bayer CropScience NV Herbicide tolerant cotton plants and methods for identifying same
MEP3508A (xx) 2005-08-24 2010-02-10 Pioneer Hi Bred Int Kompozicije koje obezbjeđuju otpornost na više hibricida i njihov postupak upotrebe
EP1934354B1 (en) 2005-10-13 2017-11-08 Monsanto Technology, LLC Methods for producing hybrid seed
RU2441366C2 (ru) * 2006-01-12 2012-02-10 Сайбас Юроп Б.В. Мутанты epsps
CA2639900C (en) * 2006-01-23 2014-08-26 Board Of Trustees Of Michigan State University Methods for breeding glyphosate resistant plants and compositions thereof
EP1996009A4 (en) 2006-03-02 2009-09-30 Athenix Corp METHODS AND COMPOSITIONS FOR ENHANCING ENZYMA ACTIVITY IN TRANSGENIC PLANTS
CA2651428A1 (en) * 2006-05-12 2007-11-22 Commonwealth Scientific And Industrial Research Organisation Enzymes for degrading herbicides
CN101490267B (zh) 2006-05-17 2013-04-17 先锋高级育种国际公司 人工植物微染色体
US9045765B2 (en) * 2006-06-09 2015-06-02 Athenix Corporation EPSP synthase genes conferring herbicide resistance
NZ573399A (en) 2006-06-13 2011-12-22 Athenix Corp Improved epsp synthases: compositions and methods of use
US7951995B2 (en) 2006-06-28 2011-05-31 Pioneer Hi-Bred International, Inc. Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof
PT2087124E (pt) * 2006-11-29 2011-07-29 Athenix Corp Epsp sintases grg23 melhoradas: composições e processos de utilização
CL2007003744A1 (es) * 2006-12-22 2008-07-11 Bayer Cropscience Ag Composicion que comprende un derivado 2-piridilmetilbenzamida y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos.
CL2007003743A1 (es) * 2006-12-22 2008-07-11 Bayer Cropscience Ag Composicion que comprende fenamidona y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos.
EP1969930A1 (de) 2007-03-12 2008-09-17 Bayer CropScience AG Phenoxyphenylamidine und deren Verwendung als Fungizide
US9199922B2 (en) * 2007-03-12 2015-12-01 Bayer Intellectual Property Gmbh Dihalophenoxyphenylamidines and use thereof as fungicides
EP1969931A1 (de) * 2007-03-12 2008-09-17 Bayer CropScience Aktiengesellschaft Fluoalkylphenylamidine und deren Verwendung als Fungizide
US20100167926A1 (en) * 2007-03-12 2010-07-01 Bayer Cropscience Ag 3-substituted phenoxyphenylamidines and use thereof as fungicides
US8080688B2 (en) * 2007-03-12 2011-12-20 Bayer Cropscience Ag 3, 4-disubstituted phenoxyphenylamidines and use thereof as fungicides
EP1969934A1 (de) 2007-03-12 2008-09-17 Bayer CropScience AG 4-Cycloalkyl-oder 4-arylsubstituierte Phenoxyphenylamidine und deren Verwendung als Fungizide
JP2010524869A (ja) 2007-04-19 2010-07-22 バイエル・クロツプサイエンス・アクチエンゲゼルシヤフト チアジアゾリルオキシフェニルアミジンおよび殺菌剤としてのこれらの使用
DE102007045955A1 (de) 2007-09-26 2009-04-09 Bayer Cropscience Ag Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
DE102007045922A1 (de) 2007-09-26 2009-04-02 Bayer Cropscience Ag Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
DE102007045919B4 (de) 2007-09-26 2018-07-05 Bayer Intellectual Property Gmbh Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
DE102007045956A1 (de) * 2007-09-26 2009-04-09 Bayer Cropscience Ag Wirkstoffkombination mit insektiziden und akariziden Eigenschaften
DE102007045920B4 (de) 2007-09-26 2018-07-05 Bayer Intellectual Property Gmbh Synergistische Wirkstoffkombinationen
DE102007045953B4 (de) 2007-09-26 2018-07-05 Bayer Intellectual Property Gmbh Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften
DE102007045957A1 (de) 2007-09-26 2009-04-09 Bayer Cropscience Ag Wirkstoffkombinationen mit insektiziden und akarziden Eigenschaften
EP2090168A1 (de) 2008-02-12 2009-08-19 Bayer CropScience AG Methode zur Verbesserung des Pflanzenwachstums
MX2010002746A (es) * 2007-10-02 2010-06-01 Bayer Cropscience Ag Procedimientos para mejorar el crecimiento de las plantas.
US8158850B2 (en) * 2007-12-19 2012-04-17 Monsanto Technology Llc Method to enhance yield and purity of hybrid crops
EP2072506A1 (de) 2007-12-21 2009-06-24 Bayer CropScience AG Thiazolyloxyphenylamidine oder Thiadiazolyloxyphenylamidine und deren Verwendung als Fungizide
AU2009210450A1 (en) 2008-02-01 2009-08-13 Athenix Corporation Directed evolution of GRG31 and GRG36 EPSP synthase enzymes
CA2714460C (en) * 2008-03-03 2016-05-17 Ms Technologies Llc Antibodies immunoreactive with mutant 5-enolpyruvylshikimate-3-phosphate synthase
EP2113172A1 (de) * 2008-04-28 2009-11-04 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
US7964774B2 (en) * 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
US7935870B2 (en) * 2008-05-14 2011-05-03 Monsanto Technology Llc Plants and seeds of spring canola variety SCV354718
US8829282B2 (en) * 2008-05-14 2014-09-09 Monsanto Technology, Llc Plants and seeds of spring canola variety SCV425044
US7947877B2 (en) * 2008-05-14 2011-05-24 Monosanto Technology LLC Plants and seeds of spring canola variety SCV328921
US8715656B2 (en) 2008-07-08 2014-05-06 Geneuro Sa Therapeutic use of specific ligand in MSRV associated diseases
EP2168434A1 (de) 2008-08-02 2010-03-31 Bayer CropScience AG Verwendung von Azolen zur Steigerung der Resistenz von Pflanzen oder Pflanzenteilen gegenüber abiotischem Stress
CA2733134A1 (en) 2008-08-08 2010-02-11 Bayer Bioscience N.V. Methods for plant fiber characterization and identification
AU2009281457A1 (en) 2008-08-14 2010-02-18 Bayer Cropscience Ag Insecticidal 4-phenyl-1H-pyrazoles
DE102008041695A1 (de) 2008-08-29 2010-03-04 Bayer Cropscience Ag Methoden zur Verbesserung des Pflanzenwachstums
AP2011005671A0 (en) 2008-09-26 2011-04-30 Basf Agrochemical Products Bv Herbicide-resistant AHAS-mutants and methods of use.
EP2201838A1 (de) 2008-12-05 2010-06-30 Bayer CropScience AG Wirkstoff-Nützlings-Kombinationen mit insektiziden und akariziden Eigenschaften
EP2198709A1 (de) 2008-12-19 2010-06-23 Bayer CropScience AG Verfahren zur Bekämpfung resistenter tierischer Schädlinge
EP2204094A1 (en) 2008-12-29 2010-07-07 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants Introduction
CN102333445B (zh) 2008-12-29 2014-09-03 拜尔农作物科学股份公司 改善利用转基因植物生产潜力的方法
EP2223602A1 (de) 2009-02-23 2010-09-01 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials genetisch modifizierter Pflanzen
EP2039771A2 (en) 2009-01-06 2009-03-25 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants
EP2039770A2 (en) 2009-01-06 2009-03-25 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants
EP2039772A2 (en) 2009-01-06 2009-03-25 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants introduction
AR074941A1 (es) 2009-01-07 2011-02-23 Bayer Cropscience Sa Plantas transplastomicas exentas de marcador de seleccion
WO2010081689A2 (en) 2009-01-19 2010-07-22 Bayer Cropscience Ag Cyclic diones and their use as insecticides, acaricides and/or fungicides
EP2227951A1 (de) 2009-01-23 2010-09-15 Bayer CropScience AG Verwendung von Enaminocarbonylverbindungen zur Bekämpfung von durch Insekten übertragenen Viren
ES2406131T3 (es) 2009-01-28 2013-06-05 Bayer Intellectual Property Gmbh Derivados fungicidas de N-cicloalquil-N-biciclometileno-carboxamina
AR075126A1 (es) * 2009-01-29 2011-03-09 Bayer Cropscience Ag Metodo para el mejor uso del potencial de produccion de plantas transgenicas
EP2218717A1 (en) 2009-02-17 2010-08-18 Bayer CropScience AG Fungicidal N-((HET)Arylethyl)thiocarboxamide derivatives
EP2398770B1 (en) 2009-02-17 2016-12-28 Bayer Intellectual Property GmbH Fungicidal n-(phenylcycloalkyl)carboxamide, n-(benzylcycloalkyl)carboxamide and thiocarboxamide derivatives
TW201031331A (en) 2009-02-19 2010-09-01 Bayer Cropscience Ag Pesticide composition comprising a tetrazolyloxime derivative and a fungicide or an insecticide active substance
DE102009001469A1 (de) 2009-03-11 2009-09-24 Bayer Cropscience Ag Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
DE102009001681A1 (de) 2009-03-20 2010-09-23 Bayer Cropscience Ag Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
DE102009001732A1 (de) 2009-03-23 2010-09-30 Bayer Cropscience Ag Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
DE102009001728A1 (de) 2009-03-23 2010-09-30 Bayer Cropscience Ag Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
DE102009001730A1 (de) 2009-03-23 2010-09-30 Bayer Cropscience Ag Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
EA020314B9 (ru) 2009-03-25 2015-03-31 Байер Кропсайенс Аг Пестицидная комбинация биологически активных веществ
EP2410849A1 (de) 2009-03-25 2012-02-01 Bayer CropScience AG Wirkstoffkombinationen mit insektiziden und akariziden eigenschaften
MA33140B1 (fr) 2009-03-25 2012-03-01 Bayer Cropscience Ag Combinaisons d'agents actifs ayant des proprietes insecticides et acaricides
EP2410848A1 (de) 2009-03-25 2012-02-01 Bayer CropScience AG Wirkstoffkombinationen mit insektiziden und akariziden eigenschaften
WO2010108504A1 (de) 2009-03-25 2010-09-30 Bayer Cropscience Ag Wirkstoffkombinationen mit insektiziden und akariziden eigenschaften
EP2232995A1 (de) 2009-03-25 2010-09-29 Bayer CropScience AG Verfahren zur verbesserten Nutzung des Produktionspotentials transgener Pflanzen
EP2239331A1 (en) 2009-04-07 2010-10-13 Bayer CropScience AG Method for improved utilization of the production potential of transgenic plants
EP2427059B1 (en) 2009-05-06 2015-06-03 Bayer Intellectual Property GmbH Cyclopentanedione compounds and their use as insecticides and acaricides
EP2251331A1 (en) 2009-05-15 2010-11-17 Bayer CropScience AG Fungicide pyrazole carboxamides derivatives
AR076839A1 (es) 2009-05-15 2011-07-13 Bayer Cropscience Ag Derivados fungicidas de pirazol carboxamidas
WO2010132214A1 (en) 2009-05-15 2010-11-18 University Of Tennessee Research Foundation Environmental stress-inducible promoter and its application in crops
EP2255626A1 (de) 2009-05-27 2010-12-01 Bayer CropScience AG Verwendung von Succinat Dehydrogenase Inhibitoren zur Steigerung der Resistenz von Pflanzen oder Pflanzenteilen gegenüber abiotischem Stress
MX2011013224A (es) 2009-06-09 2012-06-01 Pioneer Hi Bred Int Promotor de endospermo temprano y metodos de uso.
US8071848B2 (en) * 2009-06-17 2011-12-06 Monsanto Technology Llc Plants and seeds of spring canola variety SCV218328
AU2010268400A1 (en) 2009-07-01 2012-02-02 Bayer Cropscience Nv. Methods and means for obtaining plants with enhanced glyphosate tolerance
EP2453750A2 (de) * 2009-07-16 2012-05-23 Bayer CropScience AG Synergistische wirkstoffkombinationen mit phenyltriazolen
WO2011015524A2 (en) 2009-08-03 2011-02-10 Bayer Cropscience Ag Fungicide heterocycles derivatives
EP2292094A1 (en) 2009-09-02 2011-03-09 Bayer CropScience AG Active compound combinations
US8937214B2 (en) 2009-10-23 2015-01-20 Monsanto Technology Llc Methods and compositions for expression of transgenes in plants
WO2011056544A1 (en) 2009-10-26 2011-05-12 Pioneer Hi-Bred International, Inc. Somatic ovule specific promoter and methods of use
US8153132B2 (en) 2009-10-30 2012-04-10 Ms Technologies, Inc. Antibodies immunoreactive with mutant hydroxypenylpyruvate dioxygenase
RU2639530C2 (ru) * 2009-11-23 2017-12-21 Байер Кропсайенс Н.В. Элитное событие ее-gm3 и способы и наборы для идентификации такого события в биологических образцах
AR080566A1 (es) 2009-11-23 2012-04-18 Ms Technologies Llc Plantas de soja con tolerancia a herbicidas y metodos para identificarlas
EP2504442B1 (en) 2009-11-24 2014-07-16 Katholieke Universiteit Leuven, K.U. Leuven R&D Banana promoters
GB0920891D0 (en) 2009-11-27 2010-01-13 Syngenta Participations Ag Herbicidal compositions
EP2343280A1 (en) 2009-12-10 2011-07-13 Bayer CropScience AG Fungicide quinoline derivatives
JP5871813B2 (ja) 2009-12-23 2016-03-01 バイエル・インテレクチュアル・プロパティ・ゲーエムベーハーＢａｙｅｒ Ｉｎｔｅｌｌｅｃｔｕａｌ Ｐｒｏｐｅｒｔｙ Ｇｍｂｈ Ｈｐｐｄ阻害剤型除草剤に耐性の植物
AR079883A1 (es) 2009-12-23 2012-02-29 Bayer Cropscience Ag Plantas tolerantes a herbicidas inhibidores de las hppd
UY33139A (es) 2009-12-23 2011-07-29 Bayer Cropscience Ag Plantas tolerantes a herbicidas inhibidores de las hppd
WO2011076885A1 (en) 2009-12-23 2011-06-30 Bayer Cropscience Ag Plants tolerant to hppd inhibitor herbicides
CA2785225C (en) 2009-12-23 2019-01-22 Bayer Intellectual Property Gmbh Plants tolerant to hppd inhibitor herbicides.
JP5782658B2 (ja) 2009-12-28 2015-09-24 バイエル・クロップサイエンス・アクチェンゲゼルシャフト 殺菌剤ヒドロキシモイル−テトラゾール誘導体
JP5782657B2 (ja) 2009-12-28 2015-09-24 バイエル・クロップサイエンス・アクチェンゲゼルシャフト 殺菌剤ヒドロキシモイル−テトラゾール誘導体
BR112012012755A2 (pt) 2009-12-28 2015-09-08 Bayer Crospscience Ag "composto de fórmula (i), composição fungicida e método para controle do fungo fitopatogênico de culturas"
PE20121693A1 (es) 2010-01-22 2012-12-01 Bayer Ip Gmbh Combinacion de espiromesifeno y abamectina como insecticidas
US9279130B2 (en) 2010-01-25 2016-03-08 Bayer Cropscience Nv Methods for manufacturing plant cell walls comprising chitin
EP2529028A1 (en) 2010-01-26 2012-12-05 Pioneer Hi-Bred International, Inc. Polynucleotide and polypeptide sequences associated with herbicide tolerance
CN102741415A (zh) 2010-02-02 2012-10-17 拜尔农科股份公司 使用hppd抑制剂作为选择剂的大豆转化
US8148611B2 (en) 2010-02-26 2012-04-03 Monsanto Technology Llc Plants and seeds of spring canola variety SCV453784
US8143488B2 (en) 2010-02-26 2012-03-27 Monsanto Technoloy LLC Plants and seeds of spring canola variety SCV470336
US8138394B2 (en) 2010-02-26 2012-03-20 Monsanto Technology Llc Plants and seeds of spring canola variety SCV431158
JP2013521255A (ja) 2010-03-04 2013-06-10 バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング フルオロアルキル置換２−アミドベンズイミダゾールおよび植物中のストレス耐性を強化するためのその使用
US8581048B2 (en) * 2010-03-09 2013-11-12 Monsanto Technology, Llc Plants and seeds of spring canola variety SCV119103
US8153865B2 (en) * 2010-03-11 2012-04-10 Monsanto Technology Llc Plants and seeds of spring canola variety SCV152154
BR112012023551A2 (pt) 2010-03-18 2015-09-15 Bayer Ip Gmbh aril e hetaril sulfonamidas como agentes ativos contra estresse abiótico em plantas
WO2011124554A2 (de) 2010-04-06 2011-10-13 Bayer Cropscience Ag Verwendung der 4-phenylbuttersäure und/oder ihrer salze zur steigerung der stresstoleranz in pflanzen
BR112012025848A2 (pt) 2010-04-09 2015-09-08 Bayer Ip Gmbh uso de derivados do ácido (1-cianociclopropil) fenilfosfínico, os ésteres do mesmo e/ou os sais do mesmo para aumentar a tolerância de plantas a estresse abiótico.
WO2011134911A2 (en) 2010-04-28 2011-11-03 Bayer Cropscience Ag Fungicide hydroximoyl-tetrazole derivatives
WO2011134912A1 (en) 2010-04-28 2011-11-03 Bayer Cropscience Ag Fungicide hydroximoyl-heterocycles derivatives
JP2013525401A (ja) 2010-04-28 2013-06-20 バイエル・クロップサイエンス・アーゲー 殺菌剤ヒドロキシモイル−複素環誘導体
CA2799614A1 (en) 2010-05-19 2011-11-24 The Samuel Roberts Noble Foundation, Inc. Altered leaf morphology and enhanced agronomic properties in plants
PL2576517T3 (pl) 2010-06-03 2015-06-30 Bayer Ip Gmbh N-[(het)aryloalkilo)]pirazolo(tio)karboksyamidy i ich analogi heteropodstawione
UA110703C2 (uk) 2010-06-03 2016-02-10 Байєр Кропсайнс Аг Фунгіцидні похідні n-[(тризаміщений силіл)метил]-карбоксаміду
JP5730992B2 (ja) 2010-06-03 2015-06-10 バイエル・クロップサイエンス・アーゲーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ａｇ Ｎ−［（ヘタ）アリールエチル）］ピラゾール（チオ）カルボキサミド類及びそれらのヘテロ置換された類似体
WO2011154158A1 (en) 2010-06-09 2011-12-15 Bayer Bioscience N.V. Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering
MX347575B (es) 2010-06-09 2017-05-03 Bayer Cropscience N V * Metodos y medios para modificar un genoma vegetal en una secuencia de nucleotidos usada comunmente en la ingenieria genetica de genomas vegetales.
CA2804487A1 (en) 2010-07-08 2012-01-12 University Of Copenhagen Glucosinolate transporter protein and uses thereof
CN103003246B (zh) 2010-07-20 2015-11-25 拜尔农科股份公司 作为抗真菌剂的苯并环烯烃
EP2603591A1 (en) 2010-08-13 2013-06-19 Pioneer Hi-Bred International Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (hppd) activity
EP2611300B1 (de) 2010-09-03 2016-04-06 Bayer Intellectual Property GmbH Substituierte anellierte dihydropyrimidinonderivate
CN102399794A (zh) * 2010-09-08 2012-04-04 创世纪转基因技术有限公司 一种棉花epsp合成酶突变体基因及其应用
EP2460406A1 (en) 2010-12-01 2012-06-06 Bayer CropScience AG Use of fluopyram for controlling nematodes in nematode resistant crops
RU2610088C2 (ru) 2010-09-22 2017-02-07 Байер Интеллектуэль Проперти Гмбх Применение активных ингредиентов для борьбы с нематодами у сельскохозяйственных культур, резистентных к нематодам
PE20131399A1 (es) 2010-10-07 2013-12-16 Bayer Cropscience Ag Composicion fungicida que comprende un derivado de tetrazoliloxima y un derivado de tiazolilpiperidina
KR20130132816A (ko) 2010-10-21 2013-12-05 바이엘 인텔렉쳐 프로퍼티 게엠베하 1-(헤테로시클릭 카르보닐) 피페리딘
EP2630125B1 (en) 2010-10-21 2016-08-24 Bayer Intellectual Property GmbH N-benzyl heterocyclic carboxamides
JP2013542215A (ja) 2010-11-02 2013-11-21 バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー Ｎ−ヘタリールメチルピラゾリルカルボキサミド類
US9045549B2 (en) 2010-11-03 2015-06-02 The Samuel Roberts Noble Foundation, Inc. Transcription factors for modification of lignin content in plants
ES2594284T3 (es) 2010-11-10 2016-12-19 Bayer Cropscience Ag Variantes de HPPD y procedimientos de uso
EP2640191A1 (en) 2010-11-15 2013-09-25 Bayer Intellectual Property GmbH 5-halogenopyrazole(thio)carboxamides
EP2640706B1 (en) 2010-11-15 2017-03-01 Bayer Intellectual Property GmbH N-aryl pyrazole(thio)carboxamides
US9375004B2 (en) 2010-11-15 2016-06-28 Bayer Intellectual Property Gmbh 5-halogenopyrazolecarboxamides
CN103748227A (zh) 2010-11-24 2014-04-23 先锋国际良种公司 芸苔gat事件dp-073496-4及其鉴定和/或检测组合物和方法
CA2818918A1 (en) 2010-11-24 2012-05-31 Pioneer Hi-Bred International, Inc. Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof
EP2460407A1 (de) 2010-12-01 2012-06-06 Bayer CropScience AG Wirkstoffkombinationen umfassend Pyridylethylbenzamide und weitere Wirkstoffe
EP2645856A1 (en) 2010-12-01 2013-10-09 Bayer Intellectual Property GmbH Use of fluopyram for controlling nematodes in crops and for increasing yield
BR112013013377A2 (pt) * 2010-12-03 2016-11-22 Ms Technologies Llc molécula de ácido nucléico codificante de uma sintase de ácido 5-enolpiruvil-3-fosfo-chiquímico (epsps), constructo, vetor, célula de planta, planta, método para produzir uma planta tendo
CA2820706C (en) 2010-12-03 2018-05-22 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
KR102031625B1 (ko) 2010-12-03 2019-10-21 다우 아그로사이언시즈 엘엘씨 스태킹된 제초제 내성 이벤트 ８２６４.４４.０６.１, 관련된 트랜스제닉 대두 계통, 및 이의 검출
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
WO2012088227A1 (en) 2010-12-22 2012-06-28 Pioneer Hi-Bred International, Inc. Viral promoter, truncations thereof, and methods of use
CA2821509A1 (en) 2010-12-22 2012-06-28 Pioneer Hi-Bred International, Inc. Viral promoter, truncations thereof, and methods of use
EP2474542A1 (en) 2010-12-29 2012-07-11 Bayer CropScience AG Fungicide hydroximoyl-tetrazole derivatives
BR112013016755A2 (pt) 2010-12-29 2016-07-12 Bayer Intelectual Property Gmbh derivado de tetrazoiloxima de fórmula (i), composto e método para controlar fungos fitopatogênicos de culturas
EP2471363A1 (de) 2010-12-30 2012-07-04 Bayer CropScience AG Verwendung von Aryl-, Heteroaryl- und Benzylsulfonamidocarbonsäuren, -carbonsäureestern, -carbonsäureamiden und -carbonitrilen oder deren Salze zur Steigerung der Stresstoleranz in Pflanzen
CN103328635B (zh) 2011-01-04 2015-11-25 拜尔作物科学公司 纤维选择性启动子
CN102146371B (zh) * 2011-01-17 2013-08-07 杭州瑞丰生物科技有限公司 高抗草甘膦突变基因及其改良方法和应用
MX356630B (es) 2011-01-24 2018-06-06 Bayer Cropscience Nv Uso del promotor rd29 o fragmentos del mismo para expresión de transgenes inducible por estrés en algodón.
GB201101743D0 (en) 2011-02-01 2011-03-16 Syngenta Ltd Herbicidal compositions
WO2012112411A1 (en) 2011-02-15 2012-08-23 Pioneer Hi-Bred International, Inc. Root-preferred promoter and methods of use
EP2494867A1 (de) 2011-03-01 2012-09-05 Bayer CropScience AG Halogen-substituierte Verbindungen in Kombination mit Fungiziden
EP2683239A1 (en) 2011-03-10 2014-01-15 Bayer Intellectual Property GmbH Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds
BR112013023502A2 (pt) 2011-03-14 2016-08-02 Bayer Ip Gmbh composto fórmula (i), composição fungicida, método para o controle de fungos fitopatogênicos de culturas, utilização dos compostos de fórmula (i) e processo para a produção das composições
US9078446B2 (en) 2011-03-25 2015-07-14 Bayer Intellectual Property Gmbh Use of N-(tetrazol-4-yl)- or N-(triazol-3-yl)arylcarboxamides or their salts for controlling unwanted plants in areas of transgenic crop plants being tolerant to HPPD inhibitor herbicides
WO2012130684A1 (en) 2011-03-25 2012-10-04 Bayer Cropscience Ag Use of n-(1,2,5-oxadiazol-3-yl)benzamides for controlling unwanted plants in areas of transgenic crop plants being tolerant to hppd inhibitor herbicides
US8513487B2 (en) 2011-04-07 2013-08-20 Zenon LISIECZKO Plants and seeds of spring canola variety ND-662c
US20140051575A1 (en) 2011-04-08 2014-02-20 Juergen Benting Fungicide hydroximoyl-tetrazole derivatives
US8513494B2 (en) 2011-04-08 2013-08-20 Chunren Wu Plants and seeds of spring canola variety SCV695971
AR085585A1 (es) 2011-04-15 2013-10-09 Bayer Cropscience Ag Vinil- y alquinilciclohexanoles sustituidos como principios activos contra estres abiotico de plantas
AR090010A1 (es) 2011-04-15 2014-10-15 Bayer Cropscience Ag 5-(ciclohex-2-en-1-il)-penta-2,4-dienos y 5-(ciclohex-2-en-1-il)-pent-2-en-4-inos sustituidos como principios activos contra el estres abiotico de las plantas, usos y metodos de tratamiento
EP2511255A1 (de) 2011-04-15 2012-10-17 Bayer CropScience AG Substituierte Prop-2-in-1-ol- und Prop-2-en-1-ol-Derivate
AR085568A1 (es) 2011-04-15 2013-10-09 Bayer Cropscience Ag 5-(biciclo[4.1.0]hept-3-en-2-il)-penta-2,4-dienos y 5-(biciclo[4.1.0]hept-3-en-2-il)-pent-2-en-4-inos sustituidos como principios activos contra el estres abiotico de las plantas
CA2833749C (en) 2011-04-22 2019-06-04 Bayer Intellectual Property Gmbh Active compound combinations comprising a (thio)carboxamide derivative and a fungicidal compound
BR112013027635A2 (pt) * 2011-04-29 2019-09-24 Keygene Nv sequência de nucleotídeos, enzima, vetor, hospedeiro, planta ou planta transgênica ou parte da mesma, semente, métodos para fornecer uma planta (célula) com resistência a glifosato, para acentuar resistência a glifosato de uma planta (célula), e para gerar um produto de planta, produto de planta, usos de uma sequência de nucleotídeos e de uma mutação
US8507761B2 (en) 2011-05-05 2013-08-13 Teresa Huskowska Plants and seeds of spring canola variety SCV372145
US8513495B2 (en) 2011-05-10 2013-08-20 Dale Burns Plants and seeds of spring canola variety SCV291489
GB201109239D0 (en) 2011-06-01 2011-07-13 Syngenta Participations Ag Herbicidal compositions
CN103597082B (zh) 2011-06-06 2017-09-15 拜尔作物科学公司 用于在预选位点修饰植物基因组的方法和手段
WO2013006472A1 (en) 2011-07-01 2013-01-10 Monsanto Technology Llc Methods and compositions for selective regulation of protein expression
WO2013004652A1 (de) 2011-07-04 2013-01-10 Bayer Intellectual Property Gmbh Verwendung substituierter isochinolinone, isochinolindione, isochinolintrione und dihydroisochinolinone oder jeweils deren salze als wirkstoffe gegen abiotischen pflanzenstress
BR112014001808A2 (pt) 2011-07-28 2017-10-31 Genective evento vc0-01981-5 do milho tolerante ao glifosato e kit e método para a detecção do mesmo
US8785729B2 (en) 2011-08-09 2014-07-22 Nunhems, B.V. Lettuce variety redglace
US9265252B2 (en) 2011-08-10 2016-02-23 Bayer Intellectual Property Gmbh Active compound combinations comprising specific tetramic acid derivatives
MX2014001689A (es) 2011-08-12 2014-05-27 Bayer Cropscience Nv Expresion especifica de celula guardiana de transgenes en algodon.
US8759618B2 (en) 2011-08-17 2014-06-24 Stine Seed Farm, Inc. Maize event HCEM485, compositions and methods for detecting and use thereof
JP2014524455A (ja) 2011-08-22 2014-09-22 バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー 殺真菌性ヒドロキシモイル−テトラゾール誘導体
BR112014003919A2 (pt) 2011-08-22 2017-03-14 Bayer Cropscience Ag métodos e meios para modificar um genoma de planta
EP2561759A1 (en) 2011-08-26 2013-02-27 Bayer Cropscience AG Fluoroalkyl-substituted 2-amidobenzimidazoles and their effect on plant growth
CN103781353B (zh) 2011-09-09 2016-10-19 拜耳知识产权有限责任公司 用于改良植物产量的酰基高丝氨酸内酯衍生物
US8754293B2 (en) 2011-09-09 2014-06-17 Nunhems B.V. Lettuce variety intred
EP2755949B1 (en) 2011-09-12 2015-10-21 Bayer Intellectual Property GmbH Fungicidal 4-substituted-3-{phenyl[(heterocyclylmethoxy)imino]methyl}-1,2,4-oxadizol-5(4h)-one derivatives
MX362112B (es) 2011-09-16 2019-01-07 Bayer Ip Gmbh Uso de fenilpirazolin-3-carboxilatos para mejorar el rendimiento de las plantas.
EA029850B9 (ru) 2011-09-16 2018-12-28 Байер Интеллектуэль Проперти Гмбх Применение изоксадифен-этила или изоксадифена для повышения урожайности растений
UA115971C2 (uk) 2011-09-16 2018-01-25 Байєр Інтеллектуал Проперті Гмбх Застосування ацилсульфонамідів для покращення врожайності рослин
JP2014527973A (ja) 2011-09-23 2014-10-23 バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー 非生物的な植物ストレスに対する作用剤としての４−置換１−フェニルピラゾール−３−カルボン酸誘導体の使用
PL2764101T3 (pl) 2011-10-04 2017-09-29 Bayer Intellectual Property Gmbh RNAi do kontroli grzybów i lęgniowców poprzez hamowanie genu dehydrogenazy sacharopinowej
WO2013050324A1 (de) 2011-10-06 2013-04-11 Bayer Intellectual Property Gmbh Abiotischen pflanzenstress-reduzierende kombination enthaltend 4- phenylbuttersäure (4-pba) oder eines ihrer salze (komponente (a)) und eine oder mehrere ausgewählte weitere agronomisch wirksame verbindungen (komponente(n) (b)
WO2013075817A1 (en) 2011-11-21 2013-05-30 Bayer Intellectual Property Gmbh Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives
US9725414B2 (en) 2011-11-30 2017-08-08 Bayer Intellectual Property Gmbh Fungicidal N-bicycloalkyl and N-tricycloalkyl pyrazole-4-(thio)carboxamide derivatives
CN104270946B (zh) 2011-12-19 2017-05-10 拜耳农作物科学股份公司 邻氨基苯甲酸二酰胺衍生物用于防治转基因作物中的害虫的用途
WO2013096818A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11268
WO2013096810A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11482
CN104039769B (zh) 2011-12-29 2016-10-19 拜耳知识产权有限责任公司 杀真菌的3-[(1,3-噻唑-4-基甲氧基亚氨基)(苯基)甲基]-2-取代的-1,2,4-噁二唑-5(2h)-酮衍生物
KR102028903B1 (ko) 2011-12-29 2019-10-07 바이엘 인텔렉쳐 프로퍼티 게엠베하 살진균 3-[(피리딘-2-일메톡시이미노)(페닐)메틸]-2-치환-1,2,4-옥사디아졸-5(2h)-온 유도체
US9380756B2 (en) 2012-01-04 2016-07-05 Nunhems B.V. Lettuce variety multigreen 50
WO2013103371A1 (en) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Ovule specific promoter and methods of use
US9006515B2 (en) 2012-01-06 2015-04-14 Pioneer Hi Bred International Inc Pollen preferred promoters and methods of use
WO2013109754A1 (en) 2012-01-17 2013-07-25 Pioneer Hi-Bred International, Inc. Plant transcription factors, promoters and uses thereof
NZ722692A (en) 2012-02-22 2018-02-23 Bayer Ip Gmbh Use of succinate dehydrogenase inhibitors (sdhis) for controlling wood diseases in grape
KR101891320B1 (ko) 2012-02-27 2018-08-23 바이엘 인텔렉쳐 프로퍼티 게엠베하 티아졸릴이속사졸린 및 살진균제를 함유하는 활성 화합물의 배합물
WO2013138309A1 (en) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
US9783814B2 (en) 2012-03-13 2017-10-10 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
WO2013139949A1 (en) 2012-03-23 2013-09-26 Bayer Intellectual Property Gmbh Compositions comprising a strigolactame compound for enhanced plant growth and yield
EP2836489B1 (en) 2012-04-12 2016-06-29 Bayer Cropscience AG N-acyl-2-(cyclo) alkylpyrrolidines and piperidines useful as fungicides
WO2013156559A1 (en) 2012-04-20 2013-10-24 Bayer Cropscience Ag N-cycloalkyl-n-[(heterocyclylphenyl)methylene]-(thio)carboxamide derivatives
US20150080337A1 (en) 2012-04-20 2015-03-19 Bayer Cropscience N-cycloalkyl-n-[(trisubstitutedsilylphenyl)methylene]-(thio)carboxamide derivatives
CN104245940A (zh) 2012-04-23 2014-12-24 拜尔作物科学公司 植物中的靶向基因组工程
US8859857B2 (en) 2012-04-26 2014-10-14 Monsanto Technology Llc Plants and seeds of spring canola variety SCV259778
US8802935B2 (en) 2012-04-26 2014-08-12 Monsanto Technology Llc Plants and seeds of spring canola variety SCV942568
IN2014DN09529A (zh) 2012-04-26 2015-07-17 Adisseo France Sas
US8835720B2 (en) 2012-04-26 2014-09-16 Monsanto Technology Llc Plants and seeds of spring canola variety SCV967592
US8878009B2 (en) 2012-04-26 2014-11-04 Monsanto Technology, LLP Plants and seeds of spring canola variety SCV318181
JP6326043B2 (ja) 2012-05-09 2018-05-16 バイエル・クロップサイエンス・アクチェンゲゼルシャフト ５−ハロゲノピラゾールインダニルカルボキサミド類
EP2662361A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazol indanyl carboxamides
EP2662363A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole biphenylcarboxamides
EP2662370A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole benzofuranyl carboxamides
EP2662362A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazole indanyl carboxamides
EP2662364A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG Pyrazole tetrahydronaphthyl carboxamides
EP2662360A1 (en) 2012-05-09 2013-11-13 Bayer CropScience AG 5-Halogenopyrazole indanyl carboxamides
EP2847170B1 (en) 2012-05-09 2017-11-08 Bayer CropScience AG Pyrazole indanyl carboxamides
AR091104A1 (es) 2012-05-22 2015-01-14 Bayer Cropscience Ag Combinaciones de compuestos activos que comprenden un derivado lipo-quitooligosacarido y un compuesto nematicida, insecticida o fungicida
EP2859104B1 (en) 2012-06-07 2017-05-31 Dow AgroSciences LLC Construct and method for expressing transgenes using a brassica bidirectional constitutive promoter
CA2876426A1 (en) 2012-06-15 2013-12-19 E. I. Du Pont De Nemours And Company Methods and compositions involving als variants with native substrate preference
EP2871958A1 (en) 2012-07-11 2015-05-20 Bayer CropScience AG Use of fungicidal combinations for increasing the tolerance of a plant towards abiotic stress
JP6293746B2 (ja) 2012-07-11 2018-03-14 アディセオ・フランセ・エス・アー・エス ２，４−ジヒドロキシ酪酸塩の調製のための方法
ES2672883T3 (es) 2012-07-11 2018-06-18 Institut National Des Sciences Appliquees Microorganismo modificado para la producción de 1,3-propanodiol
AU2012208997B1 (en) 2012-07-30 2013-09-19 Dlf Usa Inc. An alfalfa variety named magnum salt
US20150216168A1 (en) 2012-09-05 2015-08-06 Bayer Cropscience Ag Use of substituted 2-amidobenzimidazoles, 2-amidobenzoxazoles and 2-amidobenzothiazoles or salts thereof as active substances against abiotic plant stress
EP2895601A1 (en) 2012-09-14 2015-07-22 Bayer Cropscience LP Hppd variants and methods of use
DK3622810T5 (da) 2012-09-24 2024-09-02 Seminis Vegetable Seeds Inc Fremgangsmåder og sammensætninger til forlængelse af holdbarheden af planteprodukter
US20140109259A1 (en) 2012-10-11 2014-04-17 Pioneer Hi Bred International Inc Guard Cell Promoters and Uses Thereof
EA026838B1 (ru) 2012-10-19 2017-05-31 Байер Кропсайенс Аг Способ обработки растений, пораженных грибами, устойчивыми к фунгицидам, с использованием производных карбоксамида или тиокарбоксамида
EA026839B1 (ru) 2012-10-19 2017-05-31 Байер Кропсайенс Аг Комбинации активных соединений, содержащие карбоксамидные соединения
MX2015004773A (es) 2012-10-19 2015-08-14 Bayer Cropscience Ag Metodo de promocion de crecimiento de planta usando derivados de carboxamida.
CA2888559C (en) 2012-10-19 2021-03-02 Bayer Cropscience Ag Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives
EP2735231A1 (en) 2012-11-23 2014-05-28 Bayer CropScience AG Active compound combinations
WO2014079957A1 (de) 2012-11-23 2014-05-30 Bayer Cropscience Ag Selektive inhibition der ethylensignaltransduktion
WO2014083089A1 (en) 2012-11-30 2014-06-05 Bayer Cropscience Ag Ternary fungicidal and pesticidal mixtures
EA030020B1 (ru) 2012-11-30 2018-06-29 Байер Кропсайенс Акциенгезельшафт Двойные фунгицидные смеси
EA031510B1 (ru) 2012-11-30 2019-01-31 Байер Кропсайенс Акциенгезельшафт Двойная фунгицидная смесь
BR112015012055B1 (pt) 2012-11-30 2021-01-12 Bayer Cropscience Ag composição fungicida ternária, seu processo de preparação, método para controlar um ou mais microrganismos nocivos, semente resistente a microrganismos nocivos e seu método de tratamento
CA3082683A1 (en) 2012-11-30 2014-06-05 Bayer Cropscience Ag Binary fungicidal mixtures
US20150305334A1 (en) 2012-12-05 2015-10-29 Bayer Cropscience Ag Use of substituted 1-(aryl ethynyl)-, 1-(heteroaryl ethynyl)-, 1-(heterocyclyl ethynyl)- and 1-(cycloalkenyl ethynyl)-cyclohexanols as active agents against abiotic plant stress
EP2740356A1 (de) 2012-12-05 2014-06-11 Bayer CropScience AG Substituierte (2Z)-5(1-Hydroxycyclohexyl)pent-2-en-4-insäure-Derivate
EP2740720A1 (de) 2012-12-05 2014-06-11 Bayer CropScience AG Substituierte bicyclische- und tricyclische Pent-2-en-4-insäure -Derivate und ihre Verwendung zur Steigerung der Stresstoleranz in Pflanzen
WO2014090765A1 (en) 2012-12-12 2014-06-19 Bayer Cropscience Ag Use of 1-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]-5-amino-3-trifluoromethyl)-1 h-1,2,4 tfia zole for controlling nematodes in nematode-resistant crops
AR093996A1 (es) 2012-12-18 2015-07-01 Bayer Cropscience Ag Combinaciones bactericidas y fungicidas binarias
US9428459B2 (en) 2012-12-19 2016-08-30 Bayer Cropscience Ag Difluoromethyl-nicotinic- tetrahydronaphtyl carboxamides
US20150351390A1 (en) 2012-12-21 2015-12-10 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
EP2964614A1 (en) 2013-03-07 2016-01-13 Bayer Cropscience AG Fungicidal 3-{phenyl[(heterocyclylmethoxy)imino]methyl}-heterocycle derivatives
US10221429B2 (en) 2013-03-07 2019-03-05 Bayer Cropscience Lp Toxin genes and methods for their use
US9273322B2 (en) 2013-03-12 2016-03-01 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
US9243258B2 (en) 2013-03-12 2016-01-26 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
CA2905743C (en) 2013-03-13 2021-09-28 Pioneer Hi-Bred International, Inc. Glyphosate application for weed control in brassica
US20160040149A1 (en) 2013-03-14 2016-02-11 Pioneer Hi-Bred International Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
BR112015023272A2 (pt) 2013-03-14 2017-07-18 Pioneer Hi Bred Int célula vegetal, planta, explante vegetal, semente transgênica, método para produzir uma célula vegetal tendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade de dicamba descarboxilase, método para controlar plantas daninhas em um campo contendo uma cultura e método para controlar plantas daninhas em um campo contendo uma cultura
CN105143248A (zh) 2013-03-14 2015-12-09 先锋国际良种公司 玉蜀黍胁迫相关转录因子18及其用途
CN105339380A (zh) 2013-03-14 2016-02-17 先锋国际良种公司 用以防治昆虫害虫的组合物和方法
EP2971000A4 (en) 2013-03-15 2016-11-23 Pioneer Hi Bred Int PHI-4 POLYPEPTIDES AND METHOD FOR THEIR USE
US20160017350A1 (en) 2013-03-15 2016-01-21 Pioneer Hi-Bred International, Inc. Compositions and methods of use of acc oxidase polynucleotides and polypeptides
EP2981614A1 (en) 2013-04-02 2016-02-10 Bayer CropScience NV Targeted genome engineering in eukaryotes
MX2015014365A (es) 2013-04-12 2015-12-07 Bayer Cropscience Ag Derivados de triazol novedosos.
EP2984080B1 (en) 2013-04-12 2017-08-30 Bayer CropScience Aktiengesellschaft Novel triazolinthione derivatives
BR112015026235A2 (pt) 2013-04-19 2017-10-10 Bayer Cropscience Ag método para melhorar a utilização do potencial de produção de plantas transgênicas envolvendo a aplicação de um derivado de ftaldiamida
CN105307492B (zh) 2013-04-19 2018-03-30 拜耳作物科学股份公司 二元杀虫或除虫混合物
TW201507722A (zh) 2013-04-30 2015-03-01 Bayer Cropscience Ag 做為殺線蟲劑及殺體內寄生蟲劑的ｎ－（２－鹵素－２－苯乙基）－羧醯胺類
WO2014177514A1 (en) 2013-04-30 2014-11-06 Bayer Cropscience Ag Nematicidal n-substituted phenethylcarboxamides
WO2014178932A1 (en) * 2013-05-02 2014-11-06 Syngenta Participations Ag Glyphosate resistant class i epsps genes
US10443063B2 (en) 2013-06-11 2019-10-15 Syngenta Participations Ag Methods for generating transgenic plants
WO2014206953A1 (en) 2013-06-26 2014-12-31 Bayer Cropscience Ag N-cycloalkyl-n-[(bicyclylphenyl)methylene]-(thio)carboxamide derivatives
AU2014289341A1 (en) 2013-07-09 2016-01-28 Bayer Cropscience Aktiengesellschaft Use of selected pyridone carboxamides or salts thereof as active substances against abiotic plant stress
CA2918909A1 (en) 2013-07-25 2015-01-29 Pioneer Hi-Bred International, Inc. Method for producing hybrid brassica seed
EP2837287A1 (en) 2013-08-15 2015-02-18 Bayer CropScience AG Use of prothioconazole for increasing root growth of Brassicaceae
WO2015023846A2 (en) 2013-08-16 2015-02-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2015038622A1 (en) 2013-09-11 2015-03-19 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
EP4159028A1 (en) 2013-09-13 2023-04-05 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA2925033A1 (en) 2013-09-24 2015-04-02 Bayer Cropscience Nv Hetero-transglycosylase and uses thereof
WO2015057600A1 (en) 2013-10-18 2015-04-23 E. I. Du Pont De Nemours And Company Glyphosate-n-acetyltransferase (glyat) sequences and methods of use
WO2015061548A1 (en) 2013-10-25 2015-04-30 Pioneer Hi-Bred International, Inc. Stem canker tolerant soybeans and methods of use
US10071967B2 (en) 2013-12-05 2018-09-11 Bayer Cropscience Aktiengesellschaft N-cycloalkyl-N-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives
EP3077378B1 (en) 2013-12-05 2018-11-07 Bayer CropScience Aktiengesellschaft N-cyclopropyl-n-{[2-(1-substitutedcyclopropyl)phenyl]methylene}-(thio)carboxamide derivatives
US20150173369A1 (en) 2013-12-20 2015-06-25 Dow Agrosciences Llc Synergistic herbicidal weed control and improved crop tolerance from combinations of 2,4-d-choline, glyphosate, and glufosinate in 2,4-d-, glyphosate- and glufosinate-tolerant soybeans, corn, cotton and other crop areas
BR112016018103B1 (pt) 2014-02-07 2024-01-16 E.I. Du Pont De Nemours And Company Polipeptídeo e seu uso, polinucleotídeo, composição, proteína de fusão, método para controlar uma população, método para inibir o crescimento, método para controlar a infestação, método para obtenção de uma planta ou célula vegetal, construto
MX2016010187A (es) 2014-02-07 2017-07-11 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
US10876130B2 (en) 2014-03-11 2020-12-29 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
AR100159A1 (es) 2014-04-22 2016-09-14 Du Pont Genes de anhidrasa carbónica plastídica para el incremento de aceite en semillas con expresión incrementada de dgat
AR100785A1 (es) 2014-06-09 2016-11-02 Dow Agrosciences Llc Control herbicida de maleza a partir de combinaciones de fluroxipir e inhibidores de als
US9686931B2 (en) 2014-07-07 2017-06-27 Alforex Seeds LLC Hybrid alfalfa variety named HybriForce-3400
AR101214A1 (es) 2014-07-22 2016-11-30 Bayer Cropscience Ag Ciano-cicloalquilpenta-2,4-dienos, ciano-cicloalquilpent-2-en-4-inas, ciano-heterociclilpenta-2,4-dienos y ciano-heterociclilpent-2-en-4-inas sustituidos como principios activos contra el estrés abiótico de plantas
WO2016022516A1 (en) 2014-08-08 2016-02-11 Pioneer Hi-Bred International, Inc. Ubiquitin promoters and introns and methods of use
WO2016044092A1 (en) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions and methods to control insect pests
US9648826B2 (en) 2014-09-29 2017-05-16 Alforex Seeds LLC Low lignin non-transgenic alfalfa varieties and methods for producing the same
CA2963558C (en) 2014-10-16 2023-04-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
AU2015346281B2 (en) 2014-11-12 2021-12-02 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
AR103024A1 (es) 2014-12-18 2017-04-12 Bayer Cropscience Ag Piridoncarboxamidas seleccionadas o sus sales como sustancias activas contra estrés abiótico de las plantas
US20170359965A1 (en) 2014-12-19 2017-12-21 E I Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
CN107531676A (zh) 2015-04-13 2018-01-02 拜耳作物科学股份公司 N‑环烷基‑n‑(双杂环基亚乙基)‑(硫代)羧酰胺衍生物
RU2017144238A (ru) 2015-05-19 2019-06-19 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
US10647995B2 (en) 2015-06-16 2020-05-12 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
RU2762832C2 (ru) 2015-08-06 2021-12-23 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки растительного происхождения и способы их применения
JP6873979B2 (ja) 2015-09-11 2021-05-19 バイエル・クロップサイエンス・アクチェンゲゼルシャフト Ｈｐｐｄ変異体および使用方法
CN108291236B (zh) * 2015-09-30 2022-07-26 先锋国际良种公司 植物epsp合酶和使用方法
EP3374907B1 (en) 2015-11-10 2022-10-19 Corteva Agriscience LLC Methods and systems for predicting the risk of transgene silencing
RU2758718C2 (ru) 2015-12-16 2021-11-01 Зингента Партисипейшнс Аг Участки генов и гены, ассоциированные с повышенной урожайностью у растений
BR112018012887B1 (pt) 2015-12-22 2024-02-06 Pioneer Hi-Bred International, Inc Cassete de expressão, vetor, métodos de obtenção de célula vegetal e planta transgênica, métodos para expressar um polinucleotídeo
WO2017136204A1 (en) 2016-02-05 2017-08-10 Pioneer Hi-Bred International, Inc. Genetic loci associated with brown stem rot resistance in soybean and methods of use
US11085050B2 (en) 2016-04-20 2021-08-10 Basf Agricultural Solutions Seed, Us Llc Elite event EE-GH7 and methods and kits for identifying such event in biological samples
EP3451837B1 (en) 2016-05-04 2021-08-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
BR112018076047A2 (pt) 2016-06-16 2019-03-26 Pioneer Hi Bred Int elemento de silenciamento, construto de dna, cassete de expressão, célula hospedeira, composição, célula vegetal, planta ou parte de planta, semente transgênica, método para controlar um inseto-praga de planta e kit
ES2924552T3 (es) 2016-06-24 2022-10-07 Pioneer Hi Bred Int Elementos reguladores de las plantas y métodos de uso de los mismos
CA3029271A1 (en) 2016-06-28 2018-01-04 Cellectis Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
MX2018015906A (es) 2016-07-01 2019-04-04 Pioneer Hi Bred Int Proteinas insecticidas de plantas y metodos para sus usos.
EP3269816A1 (en) 2016-07-11 2018-01-17 Kws Saat Se Development of fungal resistant crops by higs (host-induced gene silencing) mediated inhibition of gpi-anchored cell wall protein synthesis
WO2018013333A1 (en) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US20190159451A1 (en) 2016-07-29 2019-05-30 Bayer Cropscience Aktiengesellschaft Active compound combinations and methods to protect the propagation material of plants
BR112019005668A2 (pt) 2016-09-22 2019-06-04 Bayer Ag novos derivados de triazol
WO2018054829A1 (en) 2016-09-22 2018-03-29 Bayer Cropscience Aktiengesellschaft Novel triazole derivatives and their use as fungicides
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
GB201617420D0 (en) * 2016-10-14 2016-11-30 Univ Durham Herbicidal compositions
MX2019004930A (es) 2016-10-26 2019-06-06 Bayer Cropscience Ag Uso de piraziflumid para el control de sclerotinia spp en aplicaciones de tratamiento de semillas.
US11021716B2 (en) 2016-11-01 2021-06-01 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CA3234859A1 (en) 2016-11-23 2018-05-31 BASF Agricultural Solutions Seed US LLC Axmi669 and axmi991 toxin genes and methods for their use
MX2019006738A (es) 2016-12-08 2019-08-22 Bayer Cropscience Ag Uso de insecticidas para el control de gusanos.
EP3550958A4 (en) 2016-12-08 2020-10-07 Syngenta Participations AG PROCESSES FOR IMPROVING THE FREQUENCY OF TRANSFORMATION
WO2018108627A1 (de) 2016-12-12 2018-06-21 Bayer Cropscience Aktiengesellschaft Verwendung substituierter indolinylmethylsulfonamide oder deren salze zur steigerung der stresstoleranz in pflanzen
EP3332645A1 (de) 2016-12-12 2018-06-13 Bayer Cropscience AG Verwendung substituierter pyrimidindione oder jeweils deren salze als wirkstoffe gegen abiotischen pflanzenstress
CN110225974B (zh) 2016-12-22 2024-03-29 巴斯夫农业种子解决方案美国有限责任公司 使用cry14来控制线虫害虫
BR112019014720A2 (pt) 2017-01-18 2020-04-07 BASF Agricultural Solutions Seed US LLC métodos para conferir resistência a doença em uma planta e para aumentar o rendimento em uma planta
AR110757A1 (es) 2017-01-18 2019-05-02 Bayer Cropscience Lp Gen de toxina bp005 y procedimientos para su uso
EP3354738A1 (en) 2017-01-30 2018-08-01 Kws Saat Se Transgenic maize plant exhibiting increased yield and drought tolerance
US9999189B1 (en) 2017-02-28 2018-06-19 M.S. Technologies, Llc Soybean cultivar 54113122
US9961860B1 (en) 2017-02-28 2018-05-08 M.S. Technologies, Llc Soybean cultivar 52030201
US9867357B1 (en) 2017-02-28 2018-01-16 M.S. Technologies, Llc Soybean cultivar 56171900
US10058049B1 (en) 2017-02-28 2018-08-28 M.S. Technologies Llc Soybean cultivar 59104161
US9961859B1 (en) 2017-02-28 2018-05-08 M.S. Technologies, Llc Soybean cultivar 57111348
US9999190B1 (en) 2017-02-28 2018-06-19 M.S. Technologies, Llc Soybean cultivar 54172927
US9961861B1 (en) 2017-02-28 2018-05-08 M.S. Technologies, Llc Soybean cultivar 54190212
US9961858B1 (en) 2017-02-28 2018-05-08 M.S. Technologies, Llc Soybean cultivar 54062650
CA3055317A1 (en) 2017-03-07 2018-09-13 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use
WO2019025153A1 (de) 2017-07-31 2019-02-07 Bayer Cropscience Aktiengesellschaft Verwendung von substituierten n-sulfonyl-n'-aryldiaminoalkanen und n-sulfonyl-n'-heteroaryldiaminoalkanen oder deren salzen zur steigerung der stresstoleranz in pflanzen
CA3076831A1 (en) 2017-09-25 2019-03-28 Pioneer Hi-Bred International, Inc. Tissue-preferred promoters and methods of use
BR112020008096A2 (pt) 2017-10-24 2020-11-03 Basf Se método para conferir tolerância a um herbicida e planta de soja transgênica
WO2019083810A1 (en) 2017-10-24 2019-05-02 Basf Se IMPROVING HERBICIDE TOLERANCE FOR 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD) INHIBITORS BY NEGATIVE REGULATION OF HPPD EXPRESSION IN SOYBEANS
CN109112120B (zh) 2017-11-02 2019-11-12 四川天豫兴禾生物科技有限公司 一种含a138t突变的植物epsps突变体及其编码基因和应用
CN109182291B (zh) * 2017-11-02 2020-02-14 四川天豫兴禾生物科技有限公司 一种含k85突变的植物epsps突变体及其编码基因和应用
CA3092075A1 (en) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
CA3092078A1 (en) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US10499594B1 (en) 2018-05-21 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 79162140
US10485208B1 (en) 2018-05-21 2019-11-26 M.S. Technologies, L.L.C. Soybean cultivar 69311428
US10494639B1 (en) 2018-05-21 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 63301112
US10485209B1 (en) 2018-05-21 2019-11-26 M.S. Technologies, L.L.C. Soybean cultivar 72151329
US10501747B1 (en) 2018-05-21 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 77130123
US11702668B2 (en) 2018-05-22 2023-07-18 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
JP2021525774A (ja) 2018-06-04 2021-09-27 バイエル アクチェンゲゼルシャフトＢａｙｅｒ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 除草活性二環式ベンゾイルピラゾール
EP3833747A1 (en) 2018-06-28 2021-06-16 Pioneer Hi-Bred International, Inc. Methods for selecting transformed plants
CA3107382A1 (en) 2018-07-26 2020-01-30 Bayer Aktiengesellschaft Use of the succinate dehydrogenase inhibitor fluopyram for controlling root rot complex and/or seedling disease complex caused by rhizoctonia solani, fusarium species and pythium species in brassicaceae species
US10492401B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 64002217
US10448604B1 (en) 2018-08-01 2019-10-22 M.S. Technologies, L.L.C. Soybean cultivar 60111110
US10485210B1 (en) 2018-08-01 2019-11-26 M.S. Technologies, L.L.C. Soybean cultivar 64432136
US10455796B1 (en) 2018-08-01 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar 76420724
US10485212B1 (en) 2018-08-01 2019-11-26 M.S. Technologies, L.L.C. Soybean cultivar 65110742
US10455794B1 (en) 2018-08-01 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar 51284052
US10492433B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 78492244
US10492400B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 76011212
US10492403B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 70311819
US10492404B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 61332840
US10455793B1 (en) 2018-08-01 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar 69090024
US10492399B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 75001212
US10455795B1 (en) 2018-08-01 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar 79150907
US10555479B1 (en) 2018-08-01 2020-02-11 M.S. Technologies, L.L.C. Soybean cultivar 71342318
US10492402B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 63452016
US10485211B1 (en) 2018-08-01 2019-11-26 M.S. Technologies, L.L.C. Soybean cultivar 74142136
US10499582B1 (en) 2018-08-01 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 60312840
US10555478B1 (en) 2018-08-01 2020-02-11 M.S. Technologies, L.L.C. Soybean cultivar 75242840
US10542691B1 (en) 2018-08-02 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 73040436
US10499583B1 (en) 2018-08-02 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 73390208
US10506783B1 (en) 2018-08-02 2019-12-17 M.S. Technologies, L.L.C. Soybean cultivar 70391206
US10349606B1 (en) 2018-08-02 2019-07-16 M.S. Technologies, Llc Soybean cultivar 70404329
US10512229B1 (en) 2018-08-02 2019-12-24 M.S. Technologies, L.L.C. Soybean cultivar 74340613
US10492439B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 78281713
US10537076B1 (en) 2018-08-02 2020-01-21 M.S. Technologies, L.L.C. Soybean cultivar 70120311
US10524445B1 (en) 2018-08-02 2020-01-07 M.S. Technologies, L.L.C. Soybean cultivar 75052534
US10398121B1 (en) 2018-08-02 2019-09-03 M.S. Technologies, Llc Soybean cultivar 76132184
US10531620B1 (en) 2018-08-02 2020-01-14 M.S. Technologies, L.L.C. Soybean cultivar 70140849
US10537084B1 (en) 2018-08-02 2020-01-21 M.S. Technologies, L.L.C. Soybean cultivar 71201428
US10542690B1 (en) 2018-08-02 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 71052129
US10349605B1 (en) 2018-08-02 2019-07-16 M.S. Technologies, Llc Soybean cultivar 78320329
US10499596B1 (en) 2018-08-02 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 76034331
US10492440B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 76172605
US10499597B1 (en) 2018-08-02 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 74211709
US10548271B1 (en) 2018-08-02 2020-02-04 M.S. Technologies, L.L.C. Soybean cultivar 65180532
US10398120B1 (en) 2018-08-02 2019-09-03 M.S. Technologies, L.L.C. Soybean cultivar 70271905
US10492436B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 70422534
US10492438B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 72191315
US10537085B1 (en) 2018-08-02 2020-01-21 M.S. Technologies, L.L.C. Soybean cultivar 73330613
US10440926B1 (en) 2018-08-02 2019-10-15 M.S. Technologies, L.L.C. Soybean cultivar 75251428
US10499595B1 (en) 2018-08-02 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 77242824
US10440924B1 (en) 2018-08-02 2019-10-15 M.S. Technologies, L.L.C. Soybean cultivar 60431428
US10492441B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 75162223
US10448605B1 (en) 2018-08-02 2019-10-22 M.S. Technologies, L.L.C. Soybean cultivar 63332027
US10492437B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 73081781
US10440925B1 (en) 2018-08-02 2019-10-15 M.S. Technologies, L.L.C. Soybean cultivar 61414428
US10542692B1 (en) 2018-08-02 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 70262703
US10660291B2 (en) 2018-08-03 2020-05-26 M.S. Technologies, L.L.C. Soybean cultivar 76071630
US10492443B1 (en) 2018-08-03 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 70552824
US10542693B1 (en) 2018-08-03 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 74312619
US10492442B1 (en) 2018-08-03 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 64490328
US10517247B1 (en) 2018-08-03 2019-12-31 M.S. Technologies, L.L.C. Soybean cultivar 71270402
US10537077B1 (en) 2018-08-03 2020-01-21 M.S. Technologies, L.L.C. Soybean cultivar 76391606
US10537078B1 (en) 2018-08-03 2020-01-21 M.S. Technologies, L.L.C. Soybean cultivar 78221232
US10609881B2 (en) 2018-08-03 2020-04-07 M.S. Technologies, L.L.C. Soybean cultivar 74092327
US10631483B2 (en) 2018-08-03 2020-04-28 M.S. Technologies, L.L.C. Soybean cultivar 63030535
US10660286B2 (en) 2018-08-03 2020-05-26 M.S. Technologies, L.L.C. Soybean cultivar 66472542
US10631484B2 (en) 2018-08-03 2020-04-28 M.S. Technologies, L.L.C. Soybean cultivar 60310209
US10542694B1 (en) 2018-08-03 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 77290232
US10517246B1 (en) 2018-08-03 2019-12-31 M.S. Technologies, L.L.C. Soybean cultivar 61242247
US10531621B1 (en) 2018-08-03 2020-01-14 M.S. Technologies, L.L.C. Soybean cultivar 61364961
US10492444B1 (en) 2018-08-03 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 73412247
US10517245B1 (en) 2018-08-03 2019-12-31 M.S. Technologies, L.L.C. Soybean cultivar 67371612
US10477819B1 (en) 2018-08-06 2019-11-19 M.S. Technologies, L.L.C. Soybean cultivar 75162339
CN112714613A (zh) 2018-09-17 2021-04-27 拜耳公司 琥珀酸脱氢酶抑制剂氟吡菌酰胺用于在谷物中防治麦角菌和减少菌核的用途
WO2020057939A1 (en) 2018-09-17 2020-03-26 Bayer Aktiengesellschaft Use of the fungicide isoflucypram for controlling claviceps purpurea and reducing sclerotia in cereals
EP3874050A1 (en) 2018-10-31 2021-09-08 Pioneer Hi-Bred International, Inc. Compositions and methods for ochrobactrum-mediated plant transformation
EP3938521A1 (en) 2019-03-11 2022-01-19 Pioneer Hi-Bred International, Inc. Methods for clonal plant production
WO2020198408A1 (en) 2019-03-27 2020-10-01 Pioneer Hi-Bred International, Inc. Plant explant transformation
WO2020198496A1 (en) 2019-03-28 2020-10-01 Pioneer Hi-Bred International, Inc. Modified agrobacterium strains and use thereof for plant transformation
US10631511B1 (en) 2019-04-04 2020-04-28 M.S. Technologies, L.L.C. Soybean cultivar 83190332
US10667483B1 (en) 2019-04-22 2020-06-02 M.S. Technolgies, L.L.C. Soybean cultivar 88092742
US10595486B1 (en) 2019-06-13 2020-03-24 M.S. Technologies, L.L.C. Soybean cultivar 80330329
US10952395B2 (en) 2019-08-19 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 81371335
US10993402B2 (en) 2019-08-19 2021-05-04 M.S. Technologies, L.L.C. Soybean cultivar 87390112
US10945399B1 (en) 2019-08-19 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 89442841
US10945400B1 (en) 2019-08-19 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 86220335
US10966396B2 (en) 2019-08-19 2021-04-06 M.S. Technologies, L.L.C. Soybean cultivar 89192414
US10980204B2 (en) 2019-08-19 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 85010111
US10952394B2 (en) 2019-08-19 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 88390016
US10897866B1 (en) 2019-08-19 2021-01-26 M.S. Technologies, L.L.C. Soybean cultivar 81442208
US10980206B2 (en) 2019-08-19 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 86052115
US10932431B1 (en) 2019-08-19 2021-03-02 M.S. Technologies, L.L.C. Soybean cultivar 86072910
US10973197B2 (en) 2019-08-19 2021-04-13 M.S. Technologies, L.L.C. Soybean cultivar 87161800
US10945401B1 (en) 2019-08-19 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 83372609
US10993403B2 (en) 2019-08-19 2021-05-04 M.S. Technologies, L.L.C. Soybean cultivar 88042312
US11044870B2 (en) 2019-08-19 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 87011338
US10918064B1 (en) 2019-08-19 2021-02-16 M.S. Technologies, L.L.C. Soybean cultivar 84322401
US10980205B2 (en) 2019-08-19 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 81140111
US10897867B1 (en) 2019-08-19 2021-01-26 M.S. Technologies, L.L.C. Soybean cultivar 84450325
US10939651B1 (en) 2019-08-19 2021-03-09 M.S. Technologies, L.L.C. Soybean cultivar 83011212
US10980207B2 (en) 2019-08-19 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 87242903
US10932432B1 (en) 2019-08-20 2021-03-02 M.S. Technologies, L.L.C. Soybean cultivar 87222215
US11044871B2 (en) 2019-08-20 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 84340383
US11212998B2 (en) 2019-08-20 2022-01-04 M.S. Technologies, L.L.C. Soybean cultivar 88282833
US10952399B2 (en) 2019-08-20 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 80230701
US11044873B2 (en) 2019-08-20 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 88482541
US10945405B1 (en) 2019-08-20 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 81090603
US10952397B2 (en) 2019-08-20 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 86160724
US10945402B1 (en) 2019-08-20 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 88020223
US11044874B2 (en) 2019-08-20 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 83292541
US10952396B2 (en) 2019-08-20 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 88362310
US11219179B2 (en) 2019-08-20 2022-01-11 M.S. Technologies, L.L.C. Soybean cultivar 87272833
US11044872B2 (en) 2019-08-20 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 84344663
US11337394B2 (en) 2019-08-20 2022-05-24 M.S. Technologies, L.L.C. Soybean cultivar 86092833
US10945404B1 (en) 2019-08-20 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 83221630
US11044875B2 (en) 2019-08-20 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 81322943
US10952398B2 (en) 2019-08-20 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 84380724
US10945403B1 (en) 2019-08-20 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 85202128
US11172632B2 (en) 2019-08-20 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 85031644
US11006604B2 (en) 2019-08-27 2021-05-18 M.S. Technologies, L.L.C. Soybean cultivar 82431018
US11044877B2 (en) 2019-08-27 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 85262507
US11071273B2 (en) 2019-08-27 2021-07-27 M.S. Technologies, L.L.C. Soybean cultivar 86172030
US10993405B2 (en) 2019-08-27 2021-05-04 M.S. Technologies, L.L.C. Soybean cultivar 85281832
US11006605B2 (en) 2019-08-27 2021-05-18 M.S. Technologies, L.L.C. Soybean cultivar 84410120
US10897871B1 (en) 2019-08-27 2021-01-26 M.S. Technologies, L.L.C. Soybean cultivar 86240211
US11071274B2 (en) 2019-08-28 2021-07-27 M.S. Technologies, L.L.C. Soybean cultivar 83050118
US11006606B2 (en) 2019-08-28 2021-05-18 M.S. Technologies, L.L.C. Soybean cultivar 81201100
US10966399B2 (en) 2019-08-28 2021-04-06 M.S. Technologies, L.L.C. Soybean cultivar 80532336
US10966397B2 (en) 2019-08-28 2021-04-06 M.S. Technologies, L.L.C. Soybean cultivar 82151940
US10952401B1 (en) 2019-08-28 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 83292238
US10966398B2 (en) 2019-08-28 2021-04-06 M.S. Technologies, L.L.C. Soybean cultivar 86240546
US11019791B2 (en) 2019-08-28 2021-06-01 M.S. Technologies, L.L.C. Soybean cultivar 89242215
US11096364B2 (en) 2019-08-28 2021-08-24 M.S. Technologies, L.L.C. Soybean cultivar 81111940
US11076554B2 (en) 2019-08-28 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 87272107
US10986798B2 (en) 2019-08-28 2021-04-27 M.S. Technologies, L.L.C. Soybean cultivar 82230919
US10986799B2 (en) 2019-08-28 2021-04-27 M.S. Technologies, L.L.C. Soybean cultivar 81440919
US10999999B2 (en) 2019-08-28 2021-05-11 M.S. Technologies, L.L.C. Soybean cultivar 83392343
US10959391B2 (en) 2019-08-28 2021-03-30 M.S. Technologies, L.L.C. Soybean cultivar 80412336
US10986797B2 (en) 2019-08-28 2021-04-27 M.S. Technologies, L.L.C. Soybean cultivar 86440139
US10939654B1 (en) 2019-08-29 2021-03-09 M.S. Technologies, L.L.C. Soybean cultivar 81111423
US11140846B2 (en) 2019-08-29 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 88070907
US10980209B2 (en) 2019-08-29 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 87230016
US10952404B1 (en) 2019-08-29 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 87092440
US11071275B2 (en) 2019-08-29 2021-07-27 M.S. Technologies, L.L.C. Soybean cultivar 88060022
US10952402B1 (en) 2019-08-29 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 81171312
US11013198B2 (en) 2019-08-29 2021-05-25 M.S. Technologies, L.L.C. Soybean cultivar 84042612
US10905082B1 (en) 2019-08-29 2021-02-02 M.S. Technologies, L.L.C. Soybean cultivar 82212235
US11076555B2 (en) 2019-08-29 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 84490022
US11044879B2 (en) 2019-08-29 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 82352802
US10939653B1 (en) 2019-08-29 2021-03-09 M.S. Technologies, L.L.C. Soybean cultivar 88432102
US10925245B1 (en) 2019-08-29 2021-02-23 M.S. Technologies, L.L.C. Soybean cultivar 89021021
US11044878B2 (en) 2019-08-29 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 88031336
US11006607B2 (en) 2019-08-29 2021-05-18 M.S. Technologies, L.L.C. Soybean cultivar 80462534
US10952403B1 (en) 2019-08-29 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 80462430
US10980210B2 (en) 2019-08-29 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 80202604
US10912276B1 (en) 2019-08-29 2021-02-09 M.S. Technologies, L.L.C. Soybean cultivar 85161716
US11134635B2 (en) 2019-08-29 2021-10-05 M.S. Technologies, L.L.C. Soybean cultivar 82371519
US11140847B2 (en) 2019-08-29 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 80372223
US11076557B2 (en) 2019-08-29 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 83381828
US11140845B2 (en) 2019-08-29 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 88041740
US11134636B2 (en) 2019-08-29 2021-10-05 M.S. Technologies, L.L.C. Soybean cultivar 83222640
US11026391B2 (en) 2019-08-29 2021-06-08 M.S. Technologies, L.L.C. Soybean cultivar 82152612
US10952405B1 (en) 2019-08-29 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 88103440
US11076556B2 (en) 2019-08-29 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 83422133
US11000001B2 (en) 2019-08-29 2021-05-11 M.S. Technologies, L.L.C. Soybean cultivar 83271604
US11140855B2 (en) 2020-02-13 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 90420357
US11051479B1 (en) 2020-02-13 2021-07-06 M.S. Technologies, L.L.C. Soybean cultivar 94140580
US11202427B2 (en) 2020-02-13 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 94110636
US11147229B2 (en) 2020-02-13 2021-10-19 M.S. Technologies, L.L.C. Soybean cultivar 98240355
US11051480B1 (en) 2020-02-13 2021-07-06 M.S. Technologies, L.L.C. Soybean cultivar 93230440
US11076563B1 (en) 2020-02-13 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 94040702
US11051481B1 (en) 2020-02-13 2021-07-06 M.S. Technologies, L.L.C. Soybean cultivar 93020437
US11147230B2 (en) 2020-02-13 2021-10-19 M.S. Technologies, L.L.C. Soybean cultivar 91420287
US11116168B2 (en) 2020-02-13 2021-09-14 M.S. Technologies, L.L.C. Soybean cultivar 95420460
US11109557B1 (en) 2020-02-13 2021-09-07 M.S. Technologies, L.L.C. Soybean cultivar 98110162
US11076562B1 (en) 2020-02-13 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 95130401
US11134640B2 (en) 2020-02-13 2021-10-05 M.S. Technologies, L.L.C. Soybean cultivar 94220034
US11191238B2 (en) 2020-02-13 2021-12-07 M.S. Technologies, L.L.C. Soybean cultivar 99240189
US11140856B2 (en) 2020-02-21 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 93440976
US11109558B1 (en) 2020-02-21 2021-09-07 M.S. Technologies, L.L.C. Soybean cultivar 91220032
US11147231B2 (en) 2020-02-21 2021-10-19 M.S. Technologies, L.L.C. Soybean cultivar 97040540
US11166430B1 (en) 2020-06-02 2021-11-09 M.S. Technologies, L.L.C. Soybean cultivar 99120525
US11122764B1 (en) 2020-06-02 2021-09-21 M.S. Technologies, L.L.C. Soybean cultivar 91210322
US11172639B1 (en) 2020-06-02 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 99310382
US11202429B1 (en) 2020-06-02 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 91410746
US11122766B1 (en) 2020-06-02 2021-09-21 M.S. Technologies, L.L.C. Soybean cultivar 96140088
US11102951B1 (en) 2020-06-02 2021-08-31 M.S. Technologies, L.L.C. Soybean cultivar 96130264
US11172640B1 (en) 2020-06-02 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 91230357
US11172637B1 (en) 2020-06-02 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 96350326
US11140857B1 (en) 2020-06-02 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 93320341
US11213001B2 (en) 2020-06-02 2022-01-04 M.S. Technologies, L.L.C. Soybean cultivar 98320614
US11116169B1 (en) 2020-06-02 2021-09-14 M.S. Technologies, L.L.C. Soybean cultivar 92050703
US11122765B1 (en) 2020-06-02 2021-09-21 M.S. Technologies, L.L.C. Soybean cultivar 98220804
US11172638B1 (en) 2020-06-02 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 97320638
US11202430B1 (en) 2020-06-02 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 93120753
US11191242B1 (en) 2020-07-14 2021-12-07 M.S. Technologies, L.L.C. Soybean cultivar 95450804
US11202432B1 (en) 2020-07-14 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 91410530
US11202433B1 (en) 2020-07-14 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 92010858
US11116170B1 (en) 2020-07-14 2021-09-14 M.S. Technologies, L.L.C. Soybean cultivar 91250440
US11134642B1 (en) 2020-07-14 2021-10-05 M.S. Technologies, L.L.C. Soybean cultivar 98272614
US11197452B1 (en) 2020-07-14 2021-12-14 M.S. Technologies, L.L.C. Soybean cultivar 92140814
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas
US11191241B1 (en) 2020-07-14 2021-12-07 M.S. Technologies, L.L.C. Soybean cultivar 92220615
US11172642B1 (en) 2020-07-14 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 99150287
US11140858B1 (en) 2020-07-14 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 91040342
US11172643B1 (en) 2020-07-14 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 94440162
US11191240B1 (en) 2020-07-14 2021-12-07 M.S. Technologies, L.L.C. Soybean cultivar 91210615
US11172641B1 (en) 2020-07-14 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 90140287
US11252910B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 99350737
US11224183B1 (en) 2020-07-17 2022-01-18 M.S. Technologies, L.L.C. Soybean cultivar 95040275
US11219184B1 (en) 2020-07-17 2022-01-11 M.S. Technologies, L.L.C. Soybean cultivar 95250357
US11252911B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 91110447
US11259490B2 (en) 2020-07-17 2022-03-01 M.S. Technologies, L.L.C. Soybean cultivar 99250287
US11252908B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 98310437
US11252909B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 94120737
US11252914B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 95111047
US11252913B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 97240377
US11202434B1 (en) 2020-07-17 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 93140657
US11252912B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 90220377
US11202435B1 (en) 2020-07-17 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 92040765
US11229179B1 (en) 2020-07-29 2022-01-25 M.S. Technologies, L.L.C. Soybean cultivar 91410830
US11252915B1 (en) 2020-07-29 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 99040204
US11266101B2 (en) 2020-07-29 2022-03-08 M.S. Technologies, L.L.C. Soybean cultivar 96310052
US11337397B2 (en) 2020-07-29 2022-05-24 M.S. Technologies, L.L.C. Soybean cultivar 90442929
US11266103B2 (en) 2020-07-29 2022-03-08 M.S. Technologies, L.L.C. Soybean cultivar 99150754
US11259491B2 (en) 2020-07-29 2022-03-01 M.S. Technologies, L.L.C. Soybean cultivar 80540918
US11266104B2 (en) 2020-07-29 2022-03-08 M.S. Technologies, L.L.C. Soybean cultivar 99030547
US11219187B1 (en) 2020-07-29 2022-01-11 M.S. Technologies, L.L.C. Soybean cultivar 94240013
US11219185B1 (en) 2020-07-29 2022-01-11 M.S. Technologies, L.L.C. Soybean cultivar 97250069
US11266102B2 (en) 2020-07-29 2022-03-08 M.S. Technologies, L.L.C. Soybean cultivar 92220922
US11197453B1 (en) 2020-07-29 2021-12-14 M.S. Technologies, L.L.C. Soybean cultivar 95130716
US11337395B2 (en) 2020-07-29 2022-05-24 M.S. Technologies, L.L.C. Soybean cultivar 99090148
US11330782B2 (en) 2020-07-29 2022-05-17 M.S. Technologies, L.L.C. Soybean cultivar 93070018
US11134643B1 (en) 2020-07-29 2021-10-05 M.S. Technologies, L.L.C. Soybean cultivar 93410922
US11337396B2 (en) 2020-07-29 2022-05-24 M.S. Technologies, L.L.C. Soybean cultivar 92312145
US11140860B1 (en) 2020-07-29 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 96220972
US11178838B1 (en) 2020-07-29 2021-11-23 M.S. Technologies, L.L.C. Soybean cultivar 99262713
US11178837B1 (en) 2020-07-29 2021-11-23 M.S. Technologies, L.L.C. Soybean cultivar 92230102
US11140859B1 (en) 2020-07-29 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 90120947
US11219186B1 (en) 2020-07-29 2022-01-11 M.S. Technologies, L.L.C. Soybean cultivar 91120809
US11224184B1 (en) 2020-07-29 2022-01-18 M.S. Technologies, L.L.C. Soybean cultivar 93330609
US11363791B2 (en) 2020-09-25 2022-06-21 M.S. Technologies, L.L.C. Soybean cultivar 96060511
US11432513B2 (en) 2020-09-25 2022-09-06 M.S. Technologies, L.L.C. Soybean cultivar 99350040
US11369075B2 (en) 2020-09-25 2022-06-28 M.S. Technologies, L.L.C. Soybean cultivar 90392435
US11363789B2 (en) 2020-09-25 2022-06-21 M.S. Technologies, L.L.C. Soybean cultivar 90440910
US11363790B2 (en) 2020-09-25 2022-06-21 M.S. Technologies, L.L.C Soybean cultivar 91320747
US11412693B2 (en) 2020-09-25 2022-08-16 M.S. Technologies, L.L.C. Soybean cultivar 95240447
US20240002870A1 (en) 2020-09-30 2024-01-04 Pioneer Hi-Bred International, Inc. Rapid transformation of monocot leaf explants
US11540481B2 (en) 2020-12-29 2023-01-03 M.S. Technologies, L.L.C. Soybean cultivar 97282440
US11445691B2 (en) 2020-12-29 2022-09-20 M.S. Technologies, L.L.C. Soybean cultivar 92170645
US11477962B2 (en) 2020-12-29 2022-10-25 M.S. Technologies, L.L.C. Soybean cultivar 94110617
US11457602B2 (en) 2020-12-29 2022-10-04 M.S. Technologies, L.L.C. Soybean cultivar 97442034
EP4351621A1 (en) 2021-06-09 2024-04-17 Morehouse School of Medicine Hevamine-related plant composition and methods
US11716948B2 (en) 2021-09-07 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 04010758
US11825797B2 (en) 2021-09-07 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 03020534
US11678638B2 (en) 2021-09-07 2023-06-20 M.S. Technologies, L.L.C. Soybean cultivar 02050116
US11930766B2 (en) 2021-09-07 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 03220758
US11737418B2 (en) 2021-09-07 2023-08-29 M.S. Technologies, L.L.C. Soybean cultivar 00120926
US11696559B2 (en) 2021-09-07 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 08230349
US11737417B2 (en) 2021-09-07 2023-08-29 M.S. Technologies, L.L.C. Soybean cultivar 09020706
US11678637B2 (en) 2021-09-07 2023-06-20 M.S. Technologies, L.L.C. Soybean cultivar 03220116
US11818998B2 (en) 2021-09-07 2023-11-21 M.S. Technologies, L.L.C. Soybean cultivar 05370116
US11653616B2 (en) 2021-09-07 2023-05-23 M.S. Technologies, L.L.C. Soybean cultivar 03310138
US11712016B2 (en) 2021-09-07 2023-08-01 M.S. Technologies, L.L.C. Soybean cultivar 05101723
US11832575B2 (en) 2021-09-08 2023-12-05 M.S. Technologies, L.L.C. Soybean cultivar 09230307
US11825800B2 (en) 2021-09-08 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 05020705
US11766017B2 (en) 2021-09-08 2023-09-26 M.S. Technologies, L.L.C. Soybean cultivar 01430308
US11700828B2 (en) 2021-09-08 2023-07-18 M.S. Technologies, L.L.C. Soybean cultivar 00320209
US11825799B2 (en) 2021-09-08 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 01120432
US11716951B2 (en) 2021-09-08 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 04130322
US11707043B2 (en) 2021-09-08 2023-07-25 M.S. Technologies, L.L.C. Soybean cultivar 02330315
US11771039B2 (en) 2021-09-08 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 06210302
US11622528B2 (en) 2021-09-08 2023-04-11 M.S. Technologies, L.L.C. Soybean cultivar 01230324
US11825798B2 (en) 2021-09-08 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 07030530
US11690345B2 (en) 2021-09-08 2023-07-04 M.S. Technologies, L.L.C. Soybean cultivar 09150308
US11985948B2 (en) 2021-09-08 2024-05-21 M.S. Technologies, L.L.C. Soybean cultivar 04420343
US11716950B2 (en) 2021-09-08 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 02020322
US11895974B2 (en) 2021-09-08 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 08140308
US11716952B2 (en) 2021-09-08 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 04233715
US11653617B2 (en) 2021-09-08 2023-05-23 M.S. Technologies, L.L.C. Soybean cultivar 08330707
US11839191B2 (en) 2021-09-08 2023-12-12 M.S. Technologies, L.L.C. Soybean cultivar 01230720
US11647728B2 (en) 2021-09-08 2023-05-16 M.S. Technologies, L.L.C. Soybean cultivar 04420302
US11716949B2 (en) 2021-09-08 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 04130507
US11696560B2 (en) 2021-09-08 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 01440925
US11696562B2 (en) 2021-09-10 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 06320913
US11766018B2 (en) 2021-09-10 2023-09-26 M.S. Technologies, L.L.C. Soybean cultivar 08110534
US11856915B2 (en) 2021-09-10 2024-01-02 M.S. Technologies, L.L.C. Soybean cultivar 00350156
US11716955B2 (en) 2021-09-10 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 08080534
US11744213B2 (en) 2021-09-10 2023-09-05 M.S. Technologies, L.L.C. Soybean cultivar 05150332
US11716954B2 (en) 2021-09-10 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 04110156
US11744214B2 (en) 2021-09-10 2023-09-05 M.S. Technologies, L.L.C. Soybean cultivar 04040454
US11712017B2 (en) 2021-09-10 2023-08-01 M.S. Technologies, L.L.C. Soybean cultivar 02270817
US11716953B2 (en) 2021-09-10 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 02440012
US11690346B2 (en) 2021-09-10 2023-07-04 M.S. Technologies, L.L.C. Soybean cultivar 08210041
US11696561B2 (en) 2021-09-10 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 03420817
US11778971B2 (en) 2021-09-10 2023-10-10 M.S. Technologies, L.L.C. Soybean cultivar 08150118
US11785909B2 (en) 2021-09-10 2023-10-17 M.S. Technologies, L.L.C. Soybean cultivar 08080157
US11825801B2 (en) 2021-09-15 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 03120254
US11758867B2 (en) 2021-09-15 2023-09-19 M.S. Technologies, L.L.C. Soybean cultivar 01020340
US11766021B2 (en) 2021-09-15 2023-09-26 M.S. Technologies, L.L.C. Soybean cultivar 03420109
US11766020B2 (en) 2021-09-15 2023-09-26 M.S. Technologies, L.L.C. Soybean cultivar 01450536
US11895975B2 (en) 2021-09-15 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 09001515
US11771040B2 (en) 2021-09-15 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 07110300
US11771042B2 (en) 2021-09-15 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 01310539
US11758868B2 (en) 2021-09-15 2023-09-19 M.S. Technologies, L.L.C. Soybean cultivar 03130400
US11785910B2 (en) 2021-09-15 2023-10-17 M.S. Technologies, L.L.C. Soybean cultivar 00150230
US11856916B2 (en) 2021-09-15 2024-01-02 M.S. Technologies, L.L.C. Soybean cultivar 06380605
US11766019B2 (en) 2021-09-15 2023-09-26 M.S. Technologies, L.L.C. Soybean cultivar 08220628
US11771041B2 (en) 2021-09-15 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 00230222
US11778972B2 (en) 2021-09-15 2023-10-10 M.S. Technologies, L.L.C. Soybean cultivar 05120629
US11930767B2 (en) 2021-09-15 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 04150316
US11856917B2 (en) 2021-09-15 2024-01-02 M.S. Technologies, L.L.C. Soybean cultivar 02220303
US11832576B2 (en) 2021-09-15 2023-12-05 M.S. Technologies, L.L.C. Soybean cultivar 08050515
US11819002B2 (en) 2021-09-22 2023-11-21 M.S. Technologies, L.L.C. Soybean cultivar 06140706
US11819000B2 (en) 2021-09-22 2023-11-21 M.S. Technologies, L.L.C. Soybean cultivar 05030038
US11716956B2 (en) 2021-09-22 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 07090548
US11812716B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 04110707
US11832577B2 (en) 2021-09-22 2023-12-05 M.S. Technologies, L.L.C. Soybean cultivar 08070926
US11778973B2 (en) 2021-09-22 2023-10-10 M.S. Technologies, L.L.C. Soybean cultivar 00150108
US11825802B2 (en) 2021-09-22 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 05220177
US11812714B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 03330181
US11812715B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 05150847
US11812713B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 09080611
US11771043B2 (en) 2021-09-22 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 06110608
US11818999B2 (en) 2021-09-22 2023-11-21 M.S. Technologies, L.L.C. Soybean cultivar 04420349
US11819001B2 (en) 2021-09-22 2023-11-21 M.S. Technologies, L.L.C. Soybean cultivar 02180205
US11812712B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 02120535
US11825803B2 (en) 2021-09-22 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 08140870
US11778974B2 (en) 2021-09-22 2023-10-10 M.S. Technologies, L.L.C. Soybean cultivar 07050021
US11812717B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 06360802
US11925164B2 (en) 2021-10-06 2024-03-12 M.S. Technologies, L.L.C. Soybean cultivar 07370900
US11925165B2 (en) 2021-10-06 2024-03-12 M.S. Technologies, L.L.C. Soybean cultivar 03050606
US11832580B2 (en) 2021-10-06 2023-12-05 M.S. Technologies, L.L.C. Soybean cultivar 06150159
US11758870B2 (en) 2021-10-06 2023-09-19 M.S. Technologies, L.L.C. Soybean cultivar 09020446
US12035679B2 (en) 2021-10-06 2024-07-16 M.S. Technologies, L.L.C. Soybean cultivar 01310057
US11925162B2 (en) 2021-10-06 2024-03-12 M.S. Technologies, L.L.C. Soybean cultivar 01030624
US11930772B2 (en) 2021-10-06 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 04220959
US11925163B2 (en) 2021-10-06 2024-03-12 M.S. Technologies, L.L.C. Soybean cultivar 07160900
US11930771B2 (en) 2021-10-06 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 02130624
US11839194B2 (en) 2021-10-06 2023-12-12 M.S. Technologies, L.L.C. Soybean cultivar 04020201
US11832579B2 (en) 2021-10-06 2023-12-05 M.S. Technologies, L.L.C. Soybean cultivar 04120519
US12035678B2 (en) 2021-10-06 2024-07-16 M.S. Technologies, L.L.C. Soybean cultivar 03440554
US11903359B2 (en) 2021-10-06 2024-02-20 M.S. Technologies, L.L.C. Soybean cultivar 05010818
US12022793B2 (en) 2021-10-07 2024-07-02 M.S. Technologies, L.L.C. Soybean cultivar 05330516
US11930773B2 (en) 2021-10-07 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 81150353
US11930775B2 (en) 2021-10-07 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 09160202
US11882809B2 (en) 2021-10-07 2024-01-30 M.S. Technologies, L.L.C. Soybean cultivar 04110611
US11930774B2 (en) 2021-10-07 2024-03-19 M.S. Technologies. L.L.C. Soybean cultivar 03040515
US11895977B2 (en) 2021-10-07 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 03220926
US11895976B2 (en) 2021-10-07 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 04370122
US11997979B2 (en) 2021-10-07 2024-06-04 M.S. Technologies, L.L.C. Soybean cultivar 05250624
US11917975B2 (en) 2021-10-07 2024-03-05 M.S. Technologies, L.L.C. Soybean cultivar 05410624
US12029190B2 (en) 2021-10-07 2024-07-09 M.S. Technologies, L.L.C. Soybean cultivar 02220412
US11980153B2 (en) 2021-11-01 2024-05-14 M.S. Technologies, L.L.C. Soybean cultivar 02460140
US11991976B2 (en) 2021-11-01 2024-05-28 M.S. Technologies, L.L.C. Soybean cultivar 04310504
US11980154B2 (en) 2021-11-01 2024-05-14 M.S. Technologies, L.L.C. Soybean cultivar 01139005
US11950562B2 (en) 2021-11-01 2024-04-09 M.S. Technologies, L.L.C. Soybean cultivar 04230926
US11895978B2 (en) 2021-11-01 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 03140402
US11895979B2 (en) 2021-11-01 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 04420512
US11930776B2 (en) 2021-11-09 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 07081321
US11980156B2 (en) 2021-11-19 2024-05-14 M.S. Technologies, L.L.C. Soybean cultivar 88173583
US11980155B2 (en) 2021-11-19 2024-05-14 M.S. Technologies, L.L.C. Soybean cultivar 89033583
US11991978B2 (en) 2021-11-19 2024-05-28 M.S. Technologies, L.L.C. Soybean cultivar 86422336
US11991977B2 (en) 2021-11-19 2024-05-28 M.S. Technologies, L.L.C. Soybean cultivar 80101544
US12035680B2 (en) 2021-11-19 2024-07-16 M.S. Technologies, L.L.C. Soybean cultivar 87470849
US12016305B2 (en) 2022-03-15 2024-06-25 M.S. Technologies, L.L.C. Soybean cultivar 13390425
US11980157B2 (en) 2022-03-15 2024-05-14 M.S. Technologies, L.L.C. Soybean cultivar 13130617
US12016304B2 (en) 2022-03-15 2024-06-25 M.S. Technologies, L.L.C. Soybean cultivar 18120126
US12016306B2 (en) 2022-03-15 2024-06-25 M.S. Technologies, L.L.C. Soybean cultivar 11050808
US12089559B2 (en) 2022-08-31 2024-09-17 M.S. Technologies, L.L.C. Soybean cultivar 13320419
US12070007B2 (en) 2022-08-31 2024-08-27 M.S. Technologies, L.L.C. Soybean cultivar 11230247
US12089560B2 (en) 2022-08-31 2024-09-17 M.S. Technologies, L.L.C. Soybean cultivar 14240419
WO2024137438A2 (en) 2022-12-19 2024-06-27 BASF Agricultural Solutions Seed US LLC Insect toxin genes and methods for their use
EP4431609A1 (en) 2023-03-14 2024-09-18 Adisseo France S.A.S. Method for improving 2, 4 dihydroxybutyric acid production and yield

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5094945A (en) 1983-01-05 1992-03-10 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
DK175922B1 (da) * 1985-08-07 2005-07-04 Monsanto Technology Llc Glyphosat-resistente planter
EP0293538A1 (fr) * 1987-06-04 1988-12-07 Charles Guibernao Dispositif de réduction du frottement d'une côque de bateau
US5013659A (en) 1987-07-27 1991-05-07 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5605011A (en) 1986-08-26 1997-02-25 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US4971908A (en) * 1987-05-26 1990-11-20 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
US5145783A (en) 1987-05-26 1992-09-08 Monsanto Company Glyphosate-tolerant 5-endolpyruvyl-3-phosphoshikimate synthase
US5312910A (en) 1987-05-26 1994-05-17 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
US5310667A (en) 1989-07-17 1994-05-10 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US7705215B1 (en) * 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
ES2150900T3 (es) 1989-10-31 2000-12-16 Monsanto Co Promotor para plantas transgenicas.
AU7182791A (en) * 1990-01-05 1991-07-24 Cornell Research Foundation Inc. Rice actin gene and promoter
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5866775A (en) * 1990-09-28 1999-02-02 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
FR2673642B1 (fr) * 1991-03-05 1994-08-12 Rhone Poulenc Agrochimie Gene chimere comprenant un promoteur capable de conferer a une plante une tolerance accrue au glyphosate.
FR2673643B1 (fr) * 1991-03-05 1993-05-21 Rhone Poulenc Agrochimie Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide.
EP0666921A4 (en) * 1991-10-02 1996-02-07 Cornell Res Foundation Inc MONOCOT HAVING A DICOT PROMOTER WHICH MAY BE INDUCED BY INJURY.
US5593874A (en) * 1992-03-19 1997-01-14 Monsanto Company Enhanced expression in plants
FR2706909B1 (zh) * 1993-06-25 1995-09-29 Rhone Poulenc Agrochimie
DE4412643A1 (de) * 1993-08-26 1995-03-02 Putzmeister Maschf Großmanipulator, insbesondere für Autobetonpumpen, sowie Verfahren zu dessen Handhabung
FR2712302B1 (fr) * 1993-11-10 1996-01-05 Rhone Poulenc Agrochimie Eléments promoteurs de gènes chimères de tubuline alpha.
FR2736929B1 (fr) * 1995-07-19 1997-08-22 Rhone Poulenc Agrochimie Sequence adn isolee pouvant servir de zone de regulation dans un gene chimere utilisable pour la transformation des plantes
FR2751347B1 (fr) * 1996-07-16 2001-12-07 Rhone Poulenc Agrochimie Gene chimere a plusieurs genes de tolerance herbicide, cellule vegetale et plante tolerantes a plusieurs herbicides

Also Published As

Publication number Publication date
PL324572A1 (en) 1998-06-08
PT1217073E (pt) 2006-07-31
PT837944E (pt) 2006-07-31
JP2011041567A (ja) 2011-03-03
FR2736926B1 (fr) 1997-08-22
SI1217073T1 (sl) 2006-06-30
AU6619196A (en) 1997-02-18
NZ313667A (en) 2000-01-28
CA2223875A1 (en) 1997-02-06
ATE321866T1 (de) 2006-04-15
BG64628B1 (bg) 2005-09-30
SK285144B6 (sk) 2006-07-07
PL189453B1 (pl) 2005-08-31
DE69635995D1 (de) 2006-05-18
CZ295649B6 (cs) 2005-09-14
ES2255534T3 (es) 2006-07-01
EP1217073B1 (fr) 2006-03-15
MX9800562A (es) 1998-04-30
DE69635995T2 (de) 2006-11-30
DE69635913D1 (de) 2006-05-11
HUP9900463A2 (hu) 1999-05-28
KR19990029084A (ko) 1999-04-15
CU23172A3 (es) 2006-09-22
EP0837944B1 (fr) 2006-03-29
ES2256863T3 (es) 2006-07-16
IL122941A0 (en) 1998-08-16
RO120849B1 (ro) 2006-08-30
DK0837944T3 (da) 2006-05-01
BG102247A (en) 1999-04-30
BR9609792A (pt) 1999-03-16
TR199800065T1 (xx) 1998-05-21
HU0700738D0 (en) 2008-01-28
WO1997004103A3 (fr) 1997-03-20
WO1997004103A2 (fr) 1997-02-06
SI0837944T1 (sl) 2006-08-31
AP9801195A0 (en) 1998-03-31
UA75315C2 (en) 2006-04-17
FR2736926A1 (fr) 1997-01-24
HUP9900463A3 (en) 2001-11-28
EA199800138A1 (ru) 1998-10-29
EP1217073A3 (fr) 2002-09-04
EP0837944A2 (fr) 1998-04-29
DK1217073T3 (da) 2006-04-10
US6566587B1 (en) 2003-05-20
CN1196088A (zh) 1998-10-14
ATE320494T1 (de) 2006-04-15
CZ17498A3 (cs) 1998-06-17
CA2223875C (en) 2009-12-08
UA80895C2 (en) 2007-11-12
DE69635913T2 (de) 2007-05-31
AP886A (en) 2000-11-03
JP4691733B2 (ja) 2011-06-01
HU226302B1 (en) 2008-08-28
EP1217073A2 (fr) 2002-06-26
HU226089B1 (en) 2008-04-28
SK6498A3 (en) 1998-11-04
JPH11510043A (ja) 1999-09-07
OA10788A (en) 2002-12-24

Similar Documents

Publication Publication Date Title
CN1154734C (zh) 突变的5-烯醇丙酮酰莽草酸-3-磷酸合酶以及编码此蛋白的基因
CN1154741C (zh) 含有几个除草剂耐受性基因的嵌合基因、对几种除草剂具耐受性的植物细胞及植物
CN1202255C (zh) 编码具有果糖基转移酶活性的蛋白质的核酸分子及制备长链菊粉的方法
CN1024021C (zh) 含谷胱甘肽s-转移酶基因的除莠剂耐性植物
CN1245516C (zh) 编码乙酰乳酸合酶基因的基因
CN1656224A (zh) 编码乙酰乳酸合酶的基因
CN1044298A (zh) 合成杀虫的晶状蛋白质基因
CN1285875A (zh) 突变的羟基苯丙酮酸双氧化酶、基dna序列和含该基因且耐除草剂的植物的分离
CN101065490A (zh) 用于植物的启动子分子
CN1602356A (zh) 表达真菌mrp样abc转运蛋白的转基因生物
CN101044153A (zh) 用于植物中的真核翻译起始因子基因调节元件
CN1854154A (zh) 一种稻瘟病抗性相关蛋白及其编码基因与应用
CN1298853C (zh) 耐缺铁性稻类的构建
CN101037695A (zh) 一种控制水稻花粉育性基因及应用
CN1842592A (zh) 赋予植物再分化能力的基因及其应用
CN1898383A (zh) 来自地钱的不饱和脂肪酸合成系统酶基因及其利用
CN1262651C (zh) 使植物产生杂草防治化合物耐受性的方法
CN1283796C (zh) 编码半胱氨酸蛋白酶的基因和启动子及生产雄性不育水稻的方法
CN101037696A (zh) 一种水稻冷害基因及应用
CN1198921C (zh) 棉子糖合成酶基因、棉子糖的制备方法及其转化的植物
CN1207772A (zh) 修饰方法
CN1250728C (zh) 在原核细胞内产生活性异二聚体amv－rt的方法
CN1608132A (zh) 通过改进丙酮酸磷酸二激酶提高植物光合作用速度的方法
CN100339479C (zh) 参与油菜素类固醇合成的基因
CN1297666C (zh) 植物转录阻遏物,结合其的蛋白质核因子,及它们的应用

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1016648

Country of ref document: HK

C56 Change in the name or address of the patentee

Owner name: BAYER CO., LTD.

Free format text: FORMER NAME: BAYER CROPSCIENCE S.A.

Owner name: RHONE-POULENC AGRO

Free format text: FORMER NAME: RHONE-POULENC AGRO GMBH

Owner name: BAYER CROPSCIENCE S.A.

Free format text: FORMER NAME: RHONE-POULENC AGRO

CP01 Change in the name or title of a patent holder

Address after: lyon

Patentee after: Bayer LLC

Address before: lyon

Patentee before: BAYER CROPSCIENCE S.A.

Address after: lyon

Patentee after: BAYER CROPSCIENCE S.A.

Address before: lyon

Patentee before: AVENTIS CROPSCIENCE S.A.

Address after: lyon

Patentee after: AVENTIS CROPSCIENCE S.A.

Address before: lyon

Patentee before: RHONE-POULENC AGRO

CX01 Expiry of patent term

Granted publication date: 20040623

EXPY Termination of patent right or utility model