AU2014214004B2 - Methods for improved production of Rebaudioside D and Rebaudioside M - Google Patents

Methods for improved production of Rebaudioside D and Rebaudioside M Download PDF

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Publication number
AU2014214004B2
AU2014214004B2 AU2014214004A AU2014214004A AU2014214004B2 AU 2014214004 B2 AU2014214004 B2 AU 2014214004B2 AU 2014214004 A AU2014214004 A AU 2014214004A AU 2014214004 A AU2014214004 A AU 2014214004A AU 2014214004 B2 AU2014214004 B2 AU 2014214004B2
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AU2014214004A1 (en
Inventor
Federico BRIANZA
Simon CARLSEN
Louis During
Jorgen Hansen
Paula Hicks
Michael Dalgaard Mikkelsen
Kim OLSSON
Alexei OUSPENSKI
Angelika SEMMLER
Ernesto SIMON
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Danstar Ferment AG
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Priority to AU2020273296A priority patent/AU2020273296B2/en
Priority to AU2022283672A priority patent/AU2022283672B2/en
Assigned to DANSTAR FERMENT AG reassignment DANSTAR FERMENT AG Request for Assignment Assignors: EVOLVA SA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Seasonings (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AU2014214004A 2013-02-06 2014-02-06 Methods for improved production of Rebaudioside D and Rebaudioside M Active AU2014214004B2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2018220068A AU2018220068B2 (en) 2013-02-06 2018-08-22 Methods for improved production of rebaudioside D and rebaudioside M
AU2020273296A AU2020273296B2 (en) 2013-02-06 2020-11-19 Methods for improved production of rebaudioside D and rebaudioside M
AU2022283672A AU2022283672B2 (en) 2013-02-06 2022-12-06 Methods for improved production of Rebaudioside D and Rebaudioside M

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361761490P 2013-02-06 2013-02-06
US61/761,490 2013-02-06
US201361886442P 2013-10-03 2013-10-03
US61/886,442 2013-10-03
PCT/EP2014/052363 WO2014122227A2 (en) 2013-02-06 2014-02-06 Methods for improved production of rebaudioside d and rebaudioside m

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2018220068A Division AU2018220068B2 (en) 2013-02-06 2018-08-22 Methods for improved production of rebaudioside D and rebaudioside M

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AU2014214004A1 AU2014214004A1 (en) 2015-07-30
AU2014214004B2 true AU2014214004B2 (en) 2018-05-24

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AU2014214004A Active AU2014214004B2 (en) 2013-02-06 2014-02-06 Methods for improved production of Rebaudioside D and Rebaudioside M
AU2018220068A Active AU2018220068B2 (en) 2013-02-06 2018-08-22 Methods for improved production of rebaudioside D and rebaudioside M
AU2020273296A Active AU2020273296B2 (en) 2013-02-06 2020-11-19 Methods for improved production of rebaudioside D and rebaudioside M
AU2022283672A Active AU2022283672B2 (en) 2013-02-06 2022-12-06 Methods for improved production of Rebaudioside D and Rebaudioside M

Family Applications After (3)

Application Number Title Priority Date Filing Date
AU2018220068A Active AU2018220068B2 (en) 2013-02-06 2018-08-22 Methods for improved production of rebaudioside D and rebaudioside M
AU2020273296A Active AU2020273296B2 (en) 2013-02-06 2020-11-19 Methods for improved production of rebaudioside D and rebaudioside M
AU2022283672A Active AU2022283672B2 (en) 2013-02-06 2022-12-06 Methods for improved production of Rebaudioside D and Rebaudioside M

Country Status (11)

Country Link
US (4) US9957540B2 (enExample)
EP (2) EP2954058B1 (enExample)
JP (4) JP6379112B2 (enExample)
KR (1) KR20150115002A (enExample)
CN (2) CN105051195B (enExample)
AU (4) AU2014214004B2 (enExample)
CA (2) CA2899276C (enExample)
MX (1) MX369525B (enExample)
MY (2) MY199452A (enExample)
SG (2) SG11201505597SA (enExample)
WO (1) WO2014122227A2 (enExample)

Families Citing this family (111)

* Cited by examiner, † Cited by third party
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