AR094348A1 - Diferenciación de células madre embrionarias humanas a células pancreáticas endocrinas con el uso de reguladores hb9 - Google Patents

Diferenciación de células madre embrionarias humanas a células pancreáticas endocrinas con el uso de reguladores hb9

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Publication number
AR094348A1
AR094348A1 ARP140100012A ARP140100012A AR094348A1 AR 094348 A1 AR094348 A1 AR 094348A1 AR P140100012 A ARP140100012 A AR P140100012A AR P140100012 A ARP140100012 A AR P140100012A AR 094348 A1 AR094348 A1 AR 094348A1
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AR
Argentina
Prior art keywords
cells
differentiation
embryous
regulators
human
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ARP140100012A
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English (en)
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Janssen Biotech Inc
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Publication of AR094348A1 publication Critical patent/AR094348A1/es

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/19—Growth and differentiation factors [GDF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
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    • C12N2501/395—Thyroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
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    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente solicitud proporciona métodos para promover la diferenciación de células madre pluripotenciales a células del endodermo pancreático que expresan PDX1, NKX6.1 y HB9. Particularmente, los métodos incluyen el cultivo de células de la Etapa 4 a la Etapa 6 con una hormona tiroidea (por ejemplo, T3), un inhibidor de ALK5, o ambos.
ARP140100012A 2012-12-31 2014-01-03 Diferenciación de células madre embrionarias humanas a células pancreáticas endocrinas con el uso de reguladores hb9 AR094348A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261747672P 2012-12-31 2012-12-31

Publications (1)

Publication Number Publication Date
AR094348A1 true AR094348A1 (es) 2015-07-29

Family

ID=51017619

Family Applications (1)

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ARP140100012A AR094348A1 (es) 2012-12-31 2014-01-03 Diferenciación de células madre embrionarias humanas a células pancreáticas endocrinas con el uso de reguladores hb9

Country Status (17)

Country Link
US (3) US10138465B2 (es)
EP (2) EP2938723B1 (es)
JP (5) JP6557147B2 (es)
KR (2) KR102036780B1 (es)
CN (2) CN105073979B (es)
AR (1) AR094348A1 (es)
AU (4) AU2013368224B2 (es)
BR (1) BR112015015701A2 (es)
CA (1) CA2896658C (es)
DK (1) DK2938723T3 (es)
ES (1) ES2942484T3 (es)
HK (1) HK1217109A1 (es)
MX (1) MX2015008578A (es)
PH (1) PH12015501450A1 (es)
RU (1) RU2684215C2 (es)
SG (2) SG10201707811XA (es)
WO (1) WO2014105546A1 (es)

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