AR108847A1 - GENERACIÓN DE CÉLULAS MADRE PLURIPOTENTES HUMANAS DERIVADAS DE CÉLULAS FUNCIONALES b CON RESPIRACIÓN MITOCONDRIAL DEPENDIENTE DE GLUCOSA Y CON RESPUESTA EN DOS FASES DE SECRECIÓN DE INSULINA - Google Patents
GENERACIÓN DE CÉLULAS MADRE PLURIPOTENTES HUMANAS DERIVADAS DE CÉLULAS FUNCIONALES b CON RESPIRACIÓN MITOCONDRIAL DEPENDIENTE DE GLUCOSA Y CON RESPUESTA EN DOS FASES DE SECRECIÓN DE INSULINAInfo
- Publication number
- AR108847A1 AR108847A1 ARP170101710A ARP170101710A AR108847A1 AR 108847 A1 AR108847 A1 AR 108847A1 AR P170101710 A ARP170101710 A AR P170101710A AR P170101710 A ARP170101710 A AR P170101710A AR 108847 A1 AR108847 A1 AR 108847A1
- Authority
- AR
- Argentina
- Prior art keywords
- glucose
- cells
- insulin secretion
- mitocondrial
- phases
- Prior art date
Links
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title abstract 3
- 239000008103 glucose Substances 0.000 title abstract 3
- 230000001419 dependent effect Effects 0.000 title abstract 2
- 230000003914 insulin secretion Effects 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 title 1
- 230000029058 respiratory gaseous exchange Effects 0.000 title 1
- 210000000130 stem cell Anatomy 0.000 title 1
- 210000002237 B-cell of pancreatic islet Anatomy 0.000 abstract 3
- 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 abstract 1
- 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 abstract 1
- 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 abstract 1
- 101000971533 Homo sapiens Killer cell lectin-like receptor subfamily G member 1 Proteins 0.000 abstract 1
- 101000979205 Homo sapiens Transcription factor MafA Proteins 0.000 abstract 1
- 101000939387 Homo sapiens Urocortin-3 Proteins 0.000 abstract 1
- 102100021457 Killer cell lectin-like receptor subfamily G member 1 Human genes 0.000 abstract 1
- 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 abstract 1
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 abstract 1
- 102100029794 Urocortin-3 Human genes 0.000 abstract 1
- 230000004069 differentiation Effects 0.000 abstract 1
- 210000003890 endocrine cell Anatomy 0.000 abstract 1
- OGQSCIYDJSNCMY-UHFFFAOYSA-H iron(3+);methyl-dioxido-oxo-$l^{5}-arsane Chemical compound [Fe+3].[Fe+3].C[As]([O-])([O-])=O.C[As]([O-])([O-])=O.C[As]([O-])([O-])=O OGQSCIYDJSNCMY-UHFFFAOYSA-H 0.000 abstract 1
- 210000004153 islets of langerhan Anatomy 0.000 abstract 1
- 230000006540 mitochondrial respiration Effects 0.000 abstract 1
- 230000009996 pancreatic endocrine effect Effects 0.000 abstract 1
- 210000001778 pluripotent stem cell Anatomy 0.000 abstract 1
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
- C12N5/0678—Stem cells; Progenitor cells; Precursor cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/39—Pancreas; Islets of Langerhans
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Abstract
La solicitud proporciona métodos para diferenciar células endocrinas pancreáticas en células beta pancreáticas que expresen PDX1, NKX6.1, MAFA, UCN3 y SLC2A. Estas células beta pancreáticas pueden obtenerse por diferenciación escalonada de células madre pluripotentes. Las células beta pancreáticas exhiben una respiración mitocondrial dependiente de glucosa y una secreción de insulina estimulada por glucosa similar a las células de los islotes.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662352968P | 2016-06-21 | 2016-06-21 |
Publications (1)
Publication Number | Publication Date |
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AR108847A1 true AR108847A1 (es) | 2018-10-03 |
Family
ID=60659269
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP170101710A AR108847A1 (es) | 2016-06-21 | 2017-06-21 | GENERACIÓN DE CÉLULAS MADRE PLURIPOTENTES HUMANAS DERIVADAS DE CÉLULAS FUNCIONALES b CON RESPIRACIÓN MITOCONDRIAL DEPENDIENTE DE GLUCOSA Y CON RESPUESTA EN DOS FASES DE SECRECIÓN DE INSULINA |
Country Status (15)
Country | Link |
---|---|
US (2) | US10633635B2 (es) |
EP (2) | EP4194548A1 (es) |
JP (3) | JP7044726B2 (es) |
KR (4) | KR102376911B1 (es) |
CN (2) | CN109415693B (es) |
AR (1) | AR108847A1 (es) |
AU (2) | AU2017282638C1 (es) |
CA (1) | CA3027879A1 (es) |
DK (1) | DK3472303T3 (es) |
ES (1) | ES2939233T3 (es) |
MA (1) | MA45502A (es) |
RU (1) | RU2752499C2 (es) |
SG (1) | SG11201810550WA (es) |
TW (1) | TW201809266A (es) |
WO (1) | WO2017222879A1 (es) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP7284948B2 (ja) | 2017-06-14 | 2023-06-01 | バーテックス ファーマシューティカルズ インコーポレイテッド | 療法薬を送達するためのデバイスおよび方法 |
CA3081762A1 (en) * | 2017-11-15 | 2019-05-23 | Semma Therapeutics, Inc. | Islet cell manufacturing compositions and methods of use |
EP3758719A4 (en) * | 2018-03-02 | 2021-12-08 | Vertex Pharmaceuticals Inc. | METHODS FOR IMPROVING THE DIFFERENTIATION OF STEM CELLS INTO BETA CELLS |
US20210246427A1 (en) * | 2018-04-27 | 2021-08-12 | Kaneka Corporation | Method for producing pancreatic beta cells |
EP3790561A4 (en) * | 2018-05-07 | 2022-06-08 | President and Fellows of Harvard College | CELL POPULATIONS AND GENE EXPRESSION RELATED TO IN VITRO BETA CELL DIFFERENTIATION |
WO2019217875A1 (en) * | 2018-05-11 | 2019-11-14 | Cornell University | Method of differentiation of human pluripotent stem cells to monohormonal cells |
CA3108275A1 (en) | 2018-08-10 | 2020-02-13 | Vertex Pharmaceuticals Incorporated | Stem cell derived islet differentiation |
WO2020243668A1 (en) | 2019-05-31 | 2020-12-03 | W. L. Gore & Associates, Inc. | Cell encapsulation devices with controlled oxygen diffusion distances |
CA3139591C (en) | 2019-05-31 | 2024-01-16 | Timothy M. BRUHN | A biocompatible membrane composite |
JP2022534545A (ja) | 2019-05-31 | 2022-08-01 | ダブリュ.エル.ゴア アンド アソシエイツ,インコーポレイティド | 生体適合性メンブレン複合体 |
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WO2020264072A1 (en) * | 2019-06-25 | 2020-12-30 | Semma Therapeutics, Inc. | Enhanced differentiation of beta cells |
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