MX2015008619A - Suspension y agrupamiento de celulas humanas pluripotentes para la diferenciacion a celulas endocrinas pancreaticas. - Google Patents

Suspension y agrupamiento de celulas humanas pluripotentes para la diferenciacion a celulas endocrinas pancreaticas.

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Publication number
MX2015008619A
MX2015008619A MX2015008619A MX2015008619A MX2015008619A MX 2015008619 A MX2015008619 A MX 2015008619A MX 2015008619 A MX2015008619 A MX 2015008619A MX 2015008619 A MX2015008619 A MX 2015008619A MX 2015008619 A MX2015008619 A MX 2015008619A
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Mexico
Prior art keywords
cells
differentiation
clustering
suspension
human pluripotent
Prior art date
Application number
MX2015008619A
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English (en)
Inventor
Shelley Nelson
Benjamin Fryer
Daina Laniauskas
Marcia Blackmoore
Haiyun Wang
Kostadinka Lilova
Elizabeth Rosocha
Original Assignee
Janssen Biotech Inc
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Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of MX2015008619A publication Critical patent/MX2015008619A/es

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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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Abstract

La presente invención proporciona métodos para preparar agrupaciones de células madre pluripotentes agregadas para la diferenciación.
MX2015008619A 2012-12-31 2013-12-30 Suspension y agrupamiento de celulas humanas pluripotentes para la diferenciacion a celulas endocrinas pancreaticas. MX2015008619A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261747799P 2012-12-31 2012-12-31
US201361962158P 2013-11-01 2013-11-01
PCT/US2013/078191 WO2014106141A1 (en) 2012-12-31 2013-12-30 Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells

Publications (1)

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MX2015008619A true MX2015008619A (es) 2016-01-12

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MX2015008619A MX2015008619A (es) 2012-12-31 2013-12-30 Suspension y agrupamiento de celulas humanas pluripotentes para la diferenciacion a celulas endocrinas pancreaticas.

Country Status (16)

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US (1) US10377989B2 (es)
EP (2) EP4039798A1 (es)
JP (1) JP6529440B2 (es)
KR (1) KR20150103203A (es)
CN (1) CN105705634A (es)
AU (1) AU2013370228B2 (es)
BR (1) BR112015015714A2 (es)
CA (1) CA2896750A1 (es)
DK (1) DK2938722T3 (es)
ES (1) ES2906998T3 (es)
HK (1) HK1217727A1 (es)
MX (1) MX2015008619A (es)
PH (1) PH12015501477A1 (es)
RU (2) RU2687379C2 (es)
SG (2) SG10201709384SA (es)
WO (1) WO2014106141A1 (es)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20240014630A (ko) 2013-06-11 2024-02-01 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 SC-β 세포 및 조성물 그리고 그 생성 방법
MX2016005657A (es) * 2013-11-01 2017-01-05 Janssen Biotech Inc Suspensión y agrupamiento de células madre humanas pluripotentes para la diferenciación a células endocrinas pancreáticas.
CN106536718B (zh) * 2014-05-21 2021-04-27 国立大学法人京都大学 胰芽细胞的制造方法及含有胰芽细胞的胰疾病治疗剂
EP4374863A2 (en) 2014-12-18 2024-05-29 President and Fellows of Harvard College Methods for generating stem cell-derived beta cells and uses thereof
WO2016100930A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Methods for generating stem cell-derived b cells and methods of use thereof
US10443042B2 (en) 2014-12-18 2019-10-15 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
JP2016202172A (ja) * 2015-04-16 2016-12-08 国立大学法人京都大学 疑似膵島の製造方法
WO2017127921A1 (en) * 2016-01-26 2017-08-03 Centre For Commercialization Of Regenerative Medicine Method for dissociating cell aggregates
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
MA45502A (fr) 2016-06-21 2019-04-24 Janssen Biotech Inc Génération de cellules bêta fonctionnelles dérivées de cellules souches pluripotentes humaines ayant une respiration mitochondriale glucose-dépendante et une réponse en sécrétion d'insuline en deux phases
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
WO2019018818A1 (en) * 2017-07-21 2019-01-24 Semma Therapeutics, Inc. REAGGREGATION OF BETA PANCREATIC CELLS DERIVED FROM STEM CELLS
MX2020002421A (es) * 2017-09-11 2020-07-13 Novo Nordisk As Enriquecimiento de celulas que coexpresan nkx6.1 y peptido c derivadas in vitro a partir de celulas madre.
MX2020004939A (es) * 2017-11-15 2020-11-11 Semma Therapeutics Inc Fabricacion de composiciones de celulas islote y metodos de uso de las mismas.
US20200399602A1 (en) * 2018-01-18 2020-12-24 Agency For Science, Technology And Research Method for Differentiation of Human Pluripotent Stem Cell Lines in Suspension Culture
EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated ISLE DIFFERENTIATION DERIVED FROM STEM CELLS
US10724052B2 (en) 2018-09-07 2020-07-28 Crispr Therapeutics Ag Universal donor cells
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
AU2020283150B2 (en) 2019-05-31 2023-08-17 Viacyte, Inc. Cell encapsulation devices with controlled oxygen diffusion distances
US20220234006A1 (en) 2019-05-31 2022-07-28 W. L. Gore & Associates, Inc. A biocompatible membrane composite
CA3139585C (en) 2019-05-31 2024-01-23 W. L. Gore & Associates, Inc. A biocompatible membrane composite
EP4025690A1 (en) 2019-09-05 2022-07-13 CRISPR Therapeutics AG Universal donor cells
MX2022002663A (es) 2019-09-05 2022-04-07 Crispr Therapeutics Ag Celulas donantes universales.
AU2020399213A1 (en) * 2019-12-11 2022-05-05 Repairon Gmbh Expansion of stem cells in suspension in a bioreactor
JP2024503290A (ja) 2020-12-31 2024-01-25 クリスパー セラピューティクス アクチェンゲゼルシャフト ユニバーサルドナー細胞
WO2022203051A1 (ja) * 2021-03-25 2022-09-29 株式会社カネカ 多能性幹細胞集団の製造方法
CA3222122A1 (en) * 2021-06-15 2022-12-22 Lara SILVERMAN Novel methods for production of therapeutic mammalian cells and cell spheres and compositions of same
WO2023229819A1 (en) * 2022-05-24 2023-11-30 Bluerock Therapeutics Lp Methods of making oligodendrocyte progenitor cells

Family Cites Families (273)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3209652A (en) 1961-03-30 1965-10-05 Burgsmueller Karl Thread whirling method
AT326803B (de) 1968-08-26 1975-12-29 Binder Fa G Maschenware sowie verfahren zur herstellung derselben
US3935067A (en) 1974-11-22 1976-01-27 Wyo-Ben Products, Inc. Inorganic support for culture media
CA1201400A (en) 1982-04-16 1986-03-04 Joel L. Williams Chemically specific surfaces for influencing cell activity during culture
US4499802A (en) 1982-09-29 1985-02-19 Container Graphics Corporation Rotary cutting die with scrap ejection
US4537773A (en) 1983-12-05 1985-08-27 E. I. Du Pont De Nemours And Company α-Aminoboronic acid derivatives
US4557264A (en) 1984-04-09 1985-12-10 Ethicon Inc. Surgical filament from polypropylene blended with polyethylene
US5215893A (en) 1985-10-03 1993-06-01 Genentech, Inc. Nucleic acid encoding the ba chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US5089396A (en) 1985-10-03 1992-02-18 Genentech, Inc. Nucleic acid encoding β chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
US4737578A (en) 1986-02-10 1988-04-12 The Salk Institute For Biological Studies Human inhibin
US5863531A (en) 1986-04-18 1999-01-26 Advanced Tissue Sciences, Inc. In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework
US5759830A (en) 1986-11-20 1998-06-02 Massachusetts Institute Of Technology Three-dimensional fibrous scaffold containing attached cells for producing vascularized tissue in vivo
CA1340581C (en) 1986-11-20 1999-06-08 Joseph P. Vacanti Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices
US5567612A (en) 1986-11-20 1996-10-22 Massachusetts Institute Of Technology Genitourinary cell-matrix structure for implantation into a human and a method of making
NZ229354A (en) 1988-07-01 1990-09-26 Becton Dickinson Co Treating polymer surfaces with a gas plasma and then applying a layer of endothelial cells to the surface
EP0363125A3 (en) 1988-10-03 1990-08-16 Hana Biologics Inc. Proliferated pancreatic endocrine cell product and process
SU1767433A1 (ru) 1989-11-27 1992-10-07 Пермский государственный медицинский институт Способ определени инсулинорезистентности имунного генеза у больных сахарным диабетом I типа
US5837539A (en) 1990-11-16 1998-11-17 Osiris Therapeutics, Inc. Monoclonal antibodies for human mesenchymal stem cells
ES2134212T3 (es) 1991-04-25 1999-10-01 Chugai Pharmaceutical Co Ltd Anticuerpo humano reconstituido contra el receptor de la interleuquina 6 humano.
US5449383A (en) 1992-03-18 1995-09-12 Chatelier; Ronald C. Cell growth substrates
GB9206861D0 (en) 1992-03-28 1992-05-13 Univ Manchester Wound healing and treatment of fibrotic disorders
CA2114282A1 (en) 1993-01-28 1994-07-29 Lothar Schilder Multi-layered implant
JP3525221B2 (ja) 1993-02-17 2004-05-10 味の素株式会社 免疫抑制剤
AU687386B2 (en) 1993-04-08 1998-02-26 Human Cell Cultures, Inc. Cell culturing method and medium
US5523226A (en) 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
GB9310557D0 (en) 1993-05-21 1993-07-07 Smithkline Beecham Plc Novel process and apparatus
TW257671B (es) 1993-11-19 1995-09-21 Ciba Geigy
US6001647A (en) 1994-04-28 1999-12-14 Ixion Biotechnology, Inc. In vitro growth of functional islets of Langerhans and in vivo uses thereof
US5834308A (en) 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans
US6703017B1 (en) 1994-04-28 2004-03-09 Ixion Biotechnology, Inc. Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US6083903A (en) 1994-10-28 2000-07-04 Leukosite, Inc. Boronic ester and acid compounds, synthesis and uses
JP4079461B2 (ja) 1994-12-29 2008-04-23 中外製薬株式会社 Ｉｌ−６アンタゴニストを含んでなる抗腫瘍剤の作用増強剤
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5718922A (en) 1995-05-31 1998-02-17 Schepens Eye Research Institute, Inc. Intravitreal microsphere drug delivery and method of preparation
US5908782A (en) 1995-06-05 1999-06-01 Osiris Therapeutics, Inc. Chemically defined medium for human mesenchymal stem cells
US5681561A (en) 1995-06-07 1997-10-28 Life Medical Sciences, Inc. Compositions and methods for improving autologous fat grafting
EP0986635A4 (en) 1997-01-10 2001-11-07 Life Technologies Inc SERUM SUBSTITUTE FOR EMBRYONIC STEM CELLS
PT1028954E (pt) 1997-04-24 2003-11-28 Ortho Mcneil Pharm Inc Imidazoles substituidos uteis no tratamento de doencas inflamatorias
CA2294944A1 (en) 1997-07-03 1999-01-14 Osiris Therapeutics, Inc. Human mesenchymal stem cells from peripheral blood
US6670127B2 (en) 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
EP1015576B1 (en) 1997-09-16 2005-05-04 Egea Biosciences, LLC Method for the complete chemical synthesis and assembly of genes and genomes
AU1197699A (en) 1997-10-23 1999-05-10 Geron Corporation Methods and materials for the growth of primate-derived primordial stem cells
AR014195A1 (es) 1997-12-29 2001-02-07 Ortho Mcneil Pharm Inc Compuestos de trifenilpropanamida utiles para el tratamiento de procesos inflamatorios, composiciones anti-inflamatorias que los comprenden, ymetodos para prepararlos
WO1999047163A2 (en) 1998-03-18 1999-09-23 Osiris Therapeutics, Inc. Mesenchymal stem cells for prevention and treatment of immune responses in transplantation
MY132496A (en) 1998-05-11 2007-10-31 Vertex Pharma Inhibitors of p38
US6413773B1 (en) 1998-06-01 2002-07-02 The Regents Of The University Of California Phosphatidylinositol 3-kinase inhibitors as stimulators of endocrine differentiation
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6610540B1 (en) 1998-11-18 2003-08-26 California Institute Of Technology Low oxygen culturing of central nervous system progenitor cells
US6413556B1 (en) 1999-01-08 2002-07-02 Sky High, Llc Aqueous anti-apoptotic compositions
EP1144597A2 (en) 1999-01-21 2001-10-17 Vitro Diagnostics, Inc. Immortalized cell lines and methods of making the same
US6815203B1 (en) 1999-06-23 2004-11-09 Joslin Diabetes Center, Inc. Methods of making pancreatic islet cells
US6306424B1 (en) 1999-06-30 2001-10-23 Ethicon, Inc. Foam composite for the repair or regeneration of tissue
US6333029B1 (en) 1999-06-30 2001-12-25 Ethicon, Inc. Porous tissue scaffoldings for the repair of regeneration of tissue
AU7719300A (en) 1999-09-27 2001-04-30 Ixion Biotechnology, Inc. Reversal of insulin-dependent diabetes by islet-producing stem cells, islet progenitor cells and islet-like structures
US6685936B2 (en) 1999-10-12 2004-02-03 Osiris Therapeutics, Inc. Suppressor cells induced by culture with mesenchymal stem cells for treatment of immune responses in transplantation
US20030082155A1 (en) 1999-12-06 2003-05-01 Habener Joel F. Stem cells of the islets of langerhans and their use in treating diabetes mellitus
JP2003517592A (ja) 1999-12-13 2003-05-27 ザ スクリプス リサーチ インスティチュート 膵島αおよびβ前駆体の同定ならびに単離のためのマーカー
US7005252B1 (en) 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
US7439064B2 (en) 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
US6436704B1 (en) 2000-04-10 2002-08-20 Raven Biotechnologies, Inc. Human pancreatic epithelial progenitor cells and methods of isolation and use thereof
US6458589B1 (en) 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
AU2001274624A1 (en) 2000-06-26 2002-01-08 Renomedix Institute Inc. Cell fraction containing cells capable of differentiating into nervous system cells
IL155367A0 (en) 2000-10-23 2003-12-23 Smithkline Beecham Corp NOVEL 2,4,8-TRISUBSTITUTED-8h-PYRIDO[2,3,-d]PYRIMIDIN-7-ONE COMPOUNDS, PHARMACEUTICAL COMPOSITIONS COMPRISING THE SAME, PROCESSES FOR THE PREPARATION THEREOF, AND USE THEREOF IN THE PREPARATION OF MEDICAMENTS FOR TREATING CSBP/p38 KINASE MEDIATED DISEASES
ES2245994T3 (es) 2000-12-08 2006-02-01 Ortho-Mcneil Pharmaceutical, Inc. Compuestos macro-heterociclicos utilizados como inhibidores de quinasa.
BR0116468A (pt) 2000-12-08 2004-06-29 Ortho Mcneil Pharm Inc Compostos de pirrolina substituìda por indazolila como inibidores de cinase
US6599323B2 (en) 2000-12-21 2003-07-29 Ethicon, Inc. Reinforced tissue implants and methods of manufacture and use
CA2435826A1 (en) 2001-01-24 2002-08-01 The Government Of The United States Of America Differentiation of stem cells to pancreatic endocrine cells
ES2359391T3 (es) 2001-01-25 2011-05-23 The United States Of America, Represented By The Secretary, Department Of Health And Human Services Formulación de compuestos de acido boronico.
US6656488B2 (en) 2001-04-11 2003-12-02 Ethicon Endo-Surgery, Inc. Bioabsorbable bag containing bioabsorbable materials of different bioabsorption rates for tissue engineering
WO2002086107A2 (en) 2001-04-19 2002-10-31 DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung A method for differentiating stem cells into insulin-producing cells
JP4296781B2 (ja) 2001-04-24 2009-07-15 味の素株式会社 幹細胞及びその分離方法
JP2004531262A (ja) 2001-05-15 2004-10-14 ラッパポート ファミリー インスチチュート フォア リサーチ イン ザ メディカル サイエンシズ ヒト胚性幹細胞由来インスリン産生細胞
US6626950B2 (en) 2001-06-28 2003-09-30 Ethicon, Inc. Composite scaffold with post anchor for the repair and regeneration of tissue
KR100418195B1 (ko) 2001-07-05 2004-02-11 주식회사 우리기술 전력케이블의 다중절연진단장치 및 그 방법
GB0117583D0 (en) 2001-07-19 2001-09-12 Astrazeneca Ab Novel compounds
WO2003014313A2 (en) 2001-08-06 2003-02-20 Bresagen, Ltd. Alternative compositions and methods for the culture of stem cells
US6617152B2 (en) 2001-09-04 2003-09-09 Corning Inc Method for creating a cell growth surface on a polymeric substrate
EP1298201A1 (en) * 2001-09-27 2003-04-02 Cardion AG Process for the production of cells exhibiting an islet-beta-cell-like state
CA2463914A1 (en) 2001-10-18 2003-04-24 Ixion Biotechnology, Inc. Conversion of liver stem and progenitor cells to pancreatic functional cells
AU2002363659B2 (en) 2001-11-15 2008-09-25 Children's Medical Center Corporation Methods of isolation, expansion and differentiation of fetal stem cells from chorionic villus, amniotic fluid, and placenta and therapeutic uses thereof
US7033831B2 (en) 2001-12-07 2006-04-25 Geron Corporation Islet cells from human embryonic stem cells
JP4653952B2 (ja) 2001-12-07 2011-03-16 サイトリ セラピューティクス インコーポレイテッド 処理済み脂肪吸引細胞で患者を治療するためのシステムと方法
WO2003054169A1 (en) 2001-12-21 2003-07-03 Thromb-X Nv Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability
AU2002367091A1 (en) 2001-12-28 2003-07-15 Cellartis Ab A method for the establishment of a pluripotent human blastocyst-derived stem cell line
US20030162290A1 (en) 2002-01-25 2003-08-28 Kazutomo Inoue Method for inducing differentiation of embryonic stem cells into functioning cells
US20030180268A1 (en) 2002-02-05 2003-09-25 Anthony Atala Tissue engineered construct for supplementing or replacing a damaged organ
EP1498478A1 (en) 2002-04-17 2005-01-19 Otsuka Pharmaceutical Co., Ltd. Method of forming pancreatic beta cells from mesenchymal cells
US20040161419A1 (en) 2002-04-19 2004-08-19 Strom Stephen C. Placental stem cells and uses thereof
WO2003095452A1 (en) 2002-05-08 2003-11-20 Janssen Pharmaceutica N.V. Substituted pyrroline kinase inhibitors
US20060003446A1 (en) 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
EP1578925A4 (en) 2002-05-28 2006-10-11 Becton Dickinson Co METHOD FOR IN VITRO EXPANSION AND TRANSDIFFERENCING HUMAN PANCREATIC AZINUS CELLS FOR INSULIN-PRODUCING CELLS
BR0311821A (pt) 2002-06-05 2005-04-05 Janssen Pharmaceutica Nv Derivados de bisindolil-maleimid como inibidores de cinase
GB0212976D0 (en) 2002-06-06 2002-07-17 Tonejet Corp Pty Ltd Ejection method and apparatus
CN1171991C (zh) 2002-07-08 2004-10-20 徐如祥 人神经干细胞的培养方法
US6877147B2 (en) 2002-07-22 2005-04-05 Broadcom Corporation Technique to assess timing delay by use of layout quality analyzer comparison
US7838290B2 (en) 2002-07-25 2010-11-23 The Scripps Research Institute Hematopoietic stem cells and methods of treatment of neovascular eye diseases therewith
JP2005534345A (ja) 2002-07-29 2005-11-17 エス セル インターナショナル ピーティーイー リミテッド インスリン陽性、グルコース応答性細胞の分化のための多段階方法
US20040063204A1 (en) 2002-08-14 2004-04-01 Lijun Yang Bone marrow cell differentiation
US7371576B2 (en) 2002-09-06 2008-05-13 Reneuron, Inc. CD56 positive human adult pancreatic endocrine progenitor cells
US9969977B2 (en) 2002-09-20 2018-05-15 Garnet Biotherapeutics Cell populations which co-express CD49c and CD90
US20040062753A1 (en) 2002-09-27 2004-04-01 Alireza Rezania Composite scaffolds seeded with mammalian cells
AU2003285172A1 (en) 2002-11-08 2004-06-03 The Johns Hopkins University Human embryonic stem cell cultures, and compositions and methods for growing same
US7144999B2 (en) 2002-11-23 2006-12-05 Isis Pharmaceuticals, Inc. Modulation of hypoxia-inducible factor 1 alpha expression
US20060040385A1 (en) 2002-12-05 2006-02-23 Technion Research & Development Foundation Ltd. Cultured human pancreatic islets, and uses thereof
DK1572984T3 (en) 2002-12-16 2016-06-13 Technion Res & Dev Foundation FEEDER CELL-FREE, XENOPHRIC CULTIVATION SYSTEM FOR HUMAN EMBRYONAL STEM CELLS
US20050118148A1 (en) 2002-12-20 2005-06-02 Roland Stein Compositions and methods related to mammalian Maf-A
RU2359671C2 (ru) 2003-01-29 2009-06-27 Такеда Фармасьютикал Компани Лимитед Способ получения препарата с покрытием
US7976853B2 (en) 2003-01-29 2011-07-12 Takeda Pharmaceutical Company Limited Process for producing coated preparation
WO2004073633A2 (en) 2003-02-14 2004-09-02 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for modulating the development of stem cells
WO2005045001A2 (en) 2003-02-14 2005-05-19 The Board Of Trustees Of The Leland Stanford Junior University Insulin-producing cells derived from stem cells
WO2004087885A2 (en) 2003-03-27 2004-10-14 Ixion Biotechnology, Inc. Method for transdifferentiation of non-pancreatic stem cells to the pancreatic pathway
US20060194315A1 (en) 2003-03-31 2006-08-31 Condie Brian G Compositions and methods for the control, differentiaton and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway
US20090203141A1 (en) 2003-05-15 2009-08-13 Shi-Lung Lin Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant RNA agents
PL2338982T3 (pl) 2003-06-27 2016-01-29 Depuy Synthes Products Inc Komórki poporodowe pochodzące z tkanek pępowinowych i sposoby ich wytwarzania i zastosowania
IL161903A0 (en) 2003-07-17 2005-11-20 Gamida Cell Ltd Ex vivo progenitor and stem cell expansion for usein the treatment of disease of endodermally- deri ved organs
ITRM20030395A1 (it) 2003-08-12 2005-02-13 Istituto Naz Per Le Malattie Infettive Lazz Terreno di coltura per il mantenimento, la proliferazione e il differenziamento di cellule di mammifero.
US7569385B2 (en) 2003-08-14 2009-08-04 The Regents Of The University Of California Multipotent amniotic fetal stem cells
US7157275B2 (en) 2003-08-15 2007-01-02 Becton, Dickinson And Company Peptides for enhanced cell attachment and growth
US20060205072A1 (en) 2003-08-27 2006-09-14 Nobuko Uchida Enriched pancreatic stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations
WO2005058301A1 (en) 2003-12-17 2005-06-30 Allergan, Inc. Methods for treating retinoid responsive disorders using selective inhibitors of cyp26a and cyp26b
GB0329498D0 (en) 2003-12-19 2004-01-28 Novartis Ag Organic compounds
US20060030042A1 (en) 2003-12-19 2006-02-09 Ali Brivanlou Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime
US7625753B2 (en) 2003-12-23 2009-12-01 Cythera, Inc. Expansion of definitive endoderm cells
CN1946838A (zh) 2003-12-23 2007-04-11 赛瑟拉公司 定形内胚层
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
DK1709159T3 (da) 2003-12-23 2019-07-29 Viacyte Inc Definitiv endoderm
TWI334443B (en) 2003-12-31 2010-12-11 Ind Tech Res Inst Method of single cell culture of undifferentiated human embryonic stem cells
US20050233446A1 (en) 2003-12-31 2005-10-20 Parsons Xuejun H Defined media for stem cell culture
US7794704B2 (en) 2004-01-23 2010-09-14 Advanced Cell Technology, Inc. Methods for producing enriched populations of human retinal pigment epithelium cells for treatment of retinal degeneration
US20080241107A1 (en) 2004-01-23 2008-10-02 Copland Iii John A Methods and Compositions For Preparing Pancreatic Insulin Secreting Cells
US20070298453A1 (en) 2004-02-12 2007-12-27 University Of Newcastle Upon Tyne Stem Cells
JP4901471B2 (ja) 2004-02-19 2012-03-21 国立大学法人京都大学 体細胞核初期化物質のスクリーニング方法
CA2581424A1 (en) 2004-03-09 2005-09-22 Gang Xu Methods for generating insulin-producing cells
WO2005086845A2 (en) 2004-03-10 2005-09-22 Regents Of The University Of California Compositions and methods for growth of embryonic stem cells
KR101178786B1 (ko) 2004-03-23 2012-09-07 다이이찌 산쿄 가부시키가이샤 다능성 줄기세포의 증식 방법
WO2005097980A2 (en) 2004-03-26 2005-10-20 Geron Corporation New protocols for making hepatocytes from embryonic stem cells
EP1730268A2 (en) 2004-04-01 2006-12-13 Wisconsin Alumni Research Foundation Differentiation of stem cells to endoderm and pancreatic lineage
EP1740612B1 (en) 2004-04-27 2019-08-07 Viacyte, Inc. Pdx1 expressing endoderm
WO2006016999A1 (en) 2004-07-09 2006-02-16 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
CA2576872C (en) 2004-08-13 2013-11-12 University Of Georgia Research Foundation, Inc. Compositions and methods for self-renewal and differentiation in human embryonic stem cells
US20080268533A1 (en) 2004-08-25 2008-10-30 University Of Georgia Research Foundation, Inc. Methods and Compositions Utilizing Myc and Gsk3Beta to Manipulate the Pluripotency of Embryonic Stem Cells
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
KR20070058584A (ko) 2004-09-08 2007-06-08 위스콘신 얼럼나이 리서어치 화운데이션 인간 배아 줄기 세포의 배양
WO2006029197A1 (en) 2004-09-08 2006-03-16 Wisconsin Alumni Research Foundation Medium and culture of embryonic stem cells
GB2437689B (en) 2005-01-28 2009-10-28 Novathera Ltd Culture and screening methods
AU2006210955A1 (en) 2005-01-31 2006-08-10 Es Cell International Pte Ltd. Directed differentiation of embryonic stem cells and uses thereof
WO2006088867A2 (en) 2005-02-15 2006-08-24 Medistem Laboratories, Incorporated Method for expansion of stem cells
JP5265204B2 (ja) 2005-03-04 2013-08-14 ライフスキャン・インコーポレイテッド 成人膵臓由来間質細胞
GB0505970D0 (en) 2005-03-23 2005-04-27 Univ Edinburgh Culture medium containing kinase inhibitor, and uses thereof
CN100425694C (zh) 2005-04-15 2008-10-15 北京大学 诱导胚胎干细胞向胰腺细胞分化的方法
US7998938B2 (en) 2005-04-15 2011-08-16 Geron Corporation Cancer treatment by combined inhibition of proteasome and telomerase activities
WO2006114098A2 (en) 2005-04-26 2006-11-02 Aarhus Universitet Biocompatible material for surgical implants and cell guiding tissue culture surfaces
JP5092124B2 (ja) 2005-05-24 2012-12-05 国立大学法人 熊本大学 Ｅｓ細胞の分化誘導方法
AU2006202209B2 (en) 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
KR20080024194A (ko) 2005-06-10 2008-03-17 아이알엠 엘엘씨 배아 줄기 세포의 다능성을 유지하는 화합물
WO2006138433A2 (en) 2005-06-14 2006-12-28 The Regents Of The University Of California Induction of cell differentiation by class i bhlh polypeptides
EP1931764A1 (en) 2005-06-21 2008-06-18 GE Healthcare Bio-Sciences AB Method for cell culture
US9074181B2 (en) 2005-06-22 2015-07-07 Asterias Biotherapeutics, Inc. Suspension culture of human embryonic stem cells
EA017545B1 (ru) 2005-06-30 2013-01-30 Янссен Фармацевтика Н.В. Циклические анилино-пиридинотриазины в качестве ингибиторов gsk-3
DE102005031532A1 (de) * 2005-07-01 2007-01-04 Cytonet Gmbh & Co. Kg Lagermedium für Zellen
GB2443370A (en) 2005-07-29 2008-04-30 Australian Stem Cell Ct Ltd Compositions and methods for growth of pluripotent cells
WO2007016485A2 (en) 2005-07-29 2007-02-08 Athersys, Inc. Use of a gsk-3 inhibitor to maintain potency of cultured cells
WO2007025234A2 (en) 2005-08-26 2007-03-01 The Trustees Of Columbia University In The City Of New York Generation of pancreatic endocrine cells from primary duct cell cultures and methods of use for treatment of diabetes
SG177946A1 (en) 2005-08-29 2012-02-28 Technion Res & Dev Foundation Media for culturing stem cells
JP2009506769A (ja) 2005-09-02 2009-02-19 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 間充織幹細胞誘導方法
US9422521B2 (en) 2005-09-12 2016-08-23 Es Cell International Pte Ltd. Differentiation of pluripotent stem cells with a kinase inhibitor or PGI2
EP1941032A2 (en) 2005-10-14 2008-07-09 Regents Of The University Of Minnesota Differentiation of non-embryonic stem cells to cells having a pancreatic phenotype
US7732202B2 (en) 2005-10-21 2010-06-08 International Stem Cell Corporation Oxygen tension for the parthenogenic activation of human oocytes for the production of human embryonic stem cells
AU2006305879B2 (en) 2005-10-27 2012-05-10 Viacyte, Inc. PDX1-expressing dorsal and ventral foregut endoderm
CN103113463B (zh) 2005-12-13 2015-02-18 国立大学法人京都大学 核重新编程因子
WO2007082963A1 (es) 2006-01-18 2007-07-26 Fundación Instituto Valenciano De Infertilidad Líneas de células madre embrionarias humanas y métodos para usar las mismas
SG170021A1 (en) 2006-02-23 2011-04-29 Novocell Inc Compositions and methods useful for culturing differentiable cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
SG10201405380QA (en) 2006-03-02 2014-10-30 Cythera Inc Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
AU2007244675A1 (en) 2006-04-28 2007-11-08 Lifescan, Inc. Differentiation of human embryonic stem cells
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
JP5288209B6 (ja) 2006-05-02 2018-06-27 ウイスコンシン アラムニ リサーチ ファンデーション 幹細胞の内胚葉細胞および膵臓系列細胞への分化方法
US8685730B2 (en) 2006-05-02 2014-04-01 Wisconsin Alumni Research Foundation Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage
WO2007136673A2 (en) 2006-05-19 2007-11-29 Medistem Laboratories, Inc. Treatment of disc degenerative disease and compositions for same
WO2007139929A2 (en) 2006-05-25 2007-12-06 The Burnham Institute For Medical Research Methods for culture and production of single cell populations of human embryonic stem cells
CN101541953A (zh) 2006-06-02 2009-09-23 佐治亚大学研究基金会 通过从人胚胎干细胞获得的定形内胚层细胞的分化得到胰和肝内胚层细胞及组织
AU2007254766A1 (en) 2006-06-02 2007-12-13 University Of Georgia Research Foundation, Inc. Pancreatic and liver endoderm cells and tissue by differentiation of definitive endoderm cells obtained from human embryonic stems
WO2007149182A2 (en) 2006-06-19 2007-12-27 Geron Corporation Differentiation and enrichment of islet-like cells from human pluripotent stem cells
CN100494359C (zh) 2006-06-23 2009-06-03 中日友好医院 神经干细胞三维立体培养体外扩增的方法
ES2610812T3 (es) 2006-06-26 2017-05-03 Lifescan, Inc. Cultivo de células madre pluripotentes
US20080003676A1 (en) 2006-06-26 2008-01-03 Millipore Corporation Growth of embryonic stem cells
AU2007270069B2 (en) 2006-07-06 2013-05-16 Es Cell International Pte Ltd Method for stem cell culture and cells derived therefrom
WO2008013664A2 (en) 2006-07-26 2008-01-31 Cythera, Inc. Methods of producing pancreatic hormones
ES2704401T3 (es) 2006-08-02 2019-03-18 Technion Res & Dev Foundation Métodos de expansión de células madre embrionarias en un cultivo en suspensión
KR101331510B1 (ko) 2006-08-30 2013-11-20 재단법인서울대학교산학협력재단 저농도의 포도당을 함유하는 인간 배아줄기세포용 배지조성물 및 이를 이용한 인간 배아 줄기세포로부터 인슐린생산 세포 또는 세포괴로 분화시키는 방법, 그리고그로부터 유도된 인슐린 생산 세포 또는 세포괴
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
WO2008039521A2 (en) 2006-09-26 2008-04-03 Nmt Medical, Inc. Method for modifying a medical implant surface for promoting tissue growth
CA2667053C (en) 2006-10-17 2015-04-28 Stiefel Laboratories, Inc. Talarazole metabolites
US20100323442A1 (en) 2006-10-17 2010-12-23 Emmanuel Edward Baetge Modulation of the phosphatidylinositol-3-kinase pathway in the differentiation of human embryonic stem cells
CA2666789C (en) 2006-10-18 2016-11-22 Yong Zhao Embryonic-like stem cells derived from adult human peripheral blood and methods of use
WO2008056779A1 (fr) 2006-11-09 2008-05-15 Japan As Represented By The President Of International Medical Center Of Japan Procédé destiné à la culture et au passage d'une cellule souche embryonnaire de primate, et procédé destiné à induire la différenciation de la cellule souche embryonnaire
US8217027B2 (en) 2006-12-21 2012-07-10 Abbott Laboratories Sphingosine-1-phosphate receptor agonist and antagonist compounds
TW200836749A (en) 2007-01-09 2008-09-16 Vioquest Pharmaceuticals Inc Compositions including triciribine and bortezomib and derivatives thereof and methods of use thereof
EP2126045A4 (en) 2007-01-30 2010-05-26 Univ Georgia EARLY MESODERM CELLS, STABLE POPULATION OF MESENDODERM CELLS WITH THE ABILITY TO GENERATE ENDODERM AND MESODERM CELL LINES AND MULTIPOTENTIAL MIGRATION CELLS
GB0703188D0 (en) 2007-02-19 2007-03-28 Roger Land Building Large scale production of stem cells
US20090053182A1 (en) 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
WO2009006422A1 (en) 2007-06-29 2009-01-08 Stem Cell Products, Inc. Automated method and apparatus for embryonic stem cell culture
WO2009006399A1 (en) 2007-07-01 2009-01-08 Lifescan Single pluripotent stem cell culture
US20100255580A1 (en) 2007-07-18 2010-10-07 Lifesccan, Inc. Differentiation of Human Embryonic Stem Cells
CN101952415B (zh) 2007-07-31 2017-06-27 生命扫描有限公司 人胚胎干细胞的分化
CN102317443B (zh) 2007-07-31 2018-09-14 生命扫描有限公司 用人饲养细胞进行的多能干细胞分化
AU2008291930B2 (en) 2007-08-24 2014-04-17 Slotervaart Participaties Bv Compositions for the treatment of neoplastic diseases
WO2009061442A1 (en) 2007-11-06 2009-05-14 Children's Medical Center Corporation Method to produce induced pluripotent stem (ips) cells form non-embryonic human cells
CA2954431C (en) 2007-11-27 2021-08-24 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic cells
GB0800524D0 (en) * 2008-01-14 2008-02-20 Univ Brighton Cell culture system
SG154367A1 (en) 2008-01-31 2009-08-28 Es Cell Int Pte Ltd Method of differentiating stem cells
WO2009096049A1 (ja) 2008-02-01 2009-08-06 Kyoto University 人工多能性幹細胞由来分化細胞
EP2250252A2 (en) 2008-02-11 2010-11-17 Cambridge Enterprise Limited Improved reprogramming of mammalian cells, and the cells obtained
US10066203B2 (en) 2008-02-21 2018-09-04 Janssen Biotech Inc. Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
JPWO2009110215A1 (ja) 2008-03-03 2011-07-14 独立行政法人科学技術振興機構 繊毛細胞の分化誘導方法
EP2271747B1 (en) * 2008-03-17 2016-09-07 Agency for Science, Technology And Research Microcarriers for pluripotent stem cell culture
RU2359030C1 (ru) 2008-03-19 2009-06-20 Общество С Ограниченной Ответственностью "Лаборатория Клеточных Технологий" Способ получения эндотелиальных клеток из эмбриональных стволовых клеток человека (варианты)
WO2009131568A1 (en) 2008-04-21 2009-10-29 Cythera, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
US8338170B2 (en) 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
US8728812B2 (en) 2008-04-22 2014-05-20 President And Fellows Of Harvard College Compositions and methods for promoting the generation of PDX1+ pancreatic cells
US7939322B2 (en) 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
US20090298178A1 (en) 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm
DK2297319T3 (en) 2008-06-03 2015-10-19 Viacyte Inc GROWTH FACTORS FOR PREPARING DEFINITIVE ENDODERM
DE102008032236A1 (de) 2008-06-30 2010-04-01 Eberhard-Karls-Universität Tübingen Isolierung und/oder Identifizierung von Stammzellen mit adipozytärem, chondrozytärem und pankreatischem Differenzierungspotential
RU2533805C2 (ru) * 2008-06-30 2014-11-20 Сентокор Орто Байотек Инк. Дифференцирование плюрипотентных стволовых клеток, представляющих собой линию клеток человека
US20100028307A1 (en) 2008-07-31 2010-02-04 O'neil John J Pluripotent stem cell differentiation
WO2010022395A2 (en) 2008-08-22 2010-02-25 President And Fellows Of Harvard College Methods of reprogramming cells
SG160248A1 (en) * 2008-09-18 2010-04-29 Agency Science Tech & Res Use of novel monoclonal antibodies targeting human embryonic stem cells to characterize and kill induced pluripotent stem cells
WO2010051223A1 (en) 2008-10-31 2010-05-06 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
ES2727950T3 (es) 2008-10-31 2019-10-21 Janssen Biotech Inc Diferenciación de células madre embrionarias humanas en linaje endocrino pancreático
US8895300B2 (en) * 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
EP3550012A1 (en) 2008-11-04 2019-10-09 ViaCyte, Inc. Stem cell aggregate suspension compositions and methods for differentiation thereof
WO2010057039A2 (en) 2008-11-14 2010-05-20 Cythera, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
WO2010059775A1 (en) 2008-11-20 2010-05-27 Centocor Ortho Biotech Inc. Pluripotent stem cell culture on micro-carriers
JP5604766B2 (ja) 2008-12-05 2014-10-15 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 多能性細胞の神経分化のための方法および培地
GB0911060D0 (en) * 2009-06-26 2009-08-12 Ge Healthcare Uk Ltd Methods for predicting the toxicity of a chemical
CA2768720C (en) 2009-07-20 2018-12-18 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
RU2579278C2 (ru) 2009-07-20 2016-04-10 Янссен Байотек, Инк. Популяция панкреатических эндокринных клеток-предшественников для снижения концентрации глюкозы в крови и способ дифференцировки панкреатических эндодермальных клеток
RU2540021C2 (ru) 2009-07-20 2015-01-27 Янссен Байотек, Инк. Дифференцировка эмбриональных стволовых клеток человека
KR101755214B1 (ko) 2009-08-12 2017-07-07 고쿠리츠 다이가쿠 호진 교토 다이가쿠 만능줄기세포의 신경전구세포로의 분화 유도 방법
US20110104805A1 (en) 2009-10-29 2011-05-05 Centocor Ortho Biotech Inc. Pluripotent Stem Cells
CA3174901A1 (en) 2009-11-12 2011-05-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
FI20096288A0 (fi) 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
CA2784425A1 (en) * 2009-12-23 2011-06-30 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells
MX343786B (es) 2009-12-23 2016-11-22 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas.
JP5762979B2 (ja) 2009-12-29 2015-08-12 武田薬品工業株式会社 膵ホルモン産生細胞の製造法
CN102858958A (zh) 2010-02-03 2013-01-02 日本国立癌症研究中心 诱导肝干细胞及其制造方法、以及该细胞的用途
SG183400A1 (en) 2010-03-02 2012-09-27 Univ Singapore Culture additives to boost stem cell proliferation and differentiation response
CN102959076B (zh) 2010-03-31 2015-09-16 斯克里普斯研究所 重编程细胞
CN103068970A (zh) 2010-04-25 2013-04-24 西奈山医学院 来自多能细胞的前部前肠内胚层的形成
WO2011143299A2 (en) 2010-05-12 2011-11-17 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells
JP5936134B2 (ja) 2010-06-15 2016-06-15 国立大学法人京都大学 ヒト人工多能性幹細胞の選択方法
WO2011160066A1 (en) * 2010-06-17 2011-12-22 Regents Of The University Of Minnesota Production of insulin producing cells
BR122020000909B1 (pt) 2010-08-05 2021-02-23 Wisconsin Alumni Research Foundation Método para derivar uma célula tronco pluripotente induzida (ips)humana sob condições definidas
PL2611907T3 (pl) 2010-08-31 2016-11-30 Różnicowanie pluripotencjalnych komórek macierzystych
US9528090B2 (en) * 2010-08-31 2016-12-27 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
MY177150A (en) 2011-02-28 2020-09-08 Stempeutics Res Malaysia Sdn Bhd Isolation and expansion of adult stem cells, their therapeutic composition and uses thereof
WO2012170853A1 (en) * 2011-06-10 2012-12-13 Wisconsin Alumni Research Foundation ("Warf") Methods and devices for differentiating pluripotent stem cells into cells of the pancreatic lineage
US20130274184A1 (en) 2011-10-11 2013-10-17 The Trustees Of Columbia University In The City Of New York Er stress relievers in beta cell protection
US8951798B2 (en) 2011-10-13 2015-02-10 Wisconsin Alumni Research Foundation Generation of cardiomyocytes from human pluripotent stem cells
WO2013055397A1 (en) 2011-10-14 2013-04-18 Children's Medical Center Corporation Inhibition and enhancement of reprogramming by chromatin modifying enzymes
CN105143446B (zh) 2011-12-22 2020-11-03 詹森生物科技公司 人胚胎干细胞分化成单一激素胰岛素阳性细胞
US10519422B2 (en) 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
ES2690118T3 (es) 2012-06-08 2018-11-19 Janssen Biotech, Inc. Diferenciación de células madre embrionarias humanas en células endocrinas pancreáticas
JP6470687B2 (ja) 2012-09-03 2019-02-13 ノヴォ ノルディスク アー／エス 小分子を用いた多能性幹細胞からの膵臓内胚葉の作製
CN111394298A (zh) 2012-12-31 2020-07-10 詹森生物科技公司 使用hb9调节子使人胚胎干细胞分化为胰腺内分泌细胞的方法
US8987471B2 (en) 2013-02-14 2015-03-24 Allergan, Inc. Substituted dihydropyrazoles as sphingosine receptor modulators
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
TW201522637A (zh) 2013-03-15 2015-06-16 Jackson Lab 非胚胎幹細胞之單離及其用途

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US20140242693A1 (en) 2014-08-28
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