MX2020002421A - Enriquecimiento de celulas que coexpresan nkx6.1 y peptido c derivadas in vitro a partir de celulas madre. - Google Patents

Enriquecimiento de celulas que coexpresan nkx6.1 y peptido c derivadas in vitro a partir de celulas madre.

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Publication number
MX2020002421A
MX2020002421A MX2020002421A MX2020002421A MX2020002421A MX 2020002421 A MX2020002421 A MX 2020002421A MX 2020002421 A MX2020002421 A MX 2020002421A MX 2020002421 A MX2020002421 A MX 2020002421A MX 2020002421 A MX2020002421 A MX 2020002421A
Authority
MX
Mexico
Prior art keywords
cells
vitro
peptide
enrichment
stem cells
Prior art date
Application number
MX2020002421A
Other languages
English (en)
Inventor
Jeannette Schlichting Kirkegaard
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of MX2020002421A publication Critical patent/MX2020002421A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Diabetes (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Emergency Medicine (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La presente invención se relaciona con un método para enriquecer agrupaciones de células que coexpresan NKX6.1 y péptido C derivadas in vitro a partir de células madre, el método comprende las etapas de disociar las agrupaciones de células endocrinas en células individuales, tratar las células individuales con medio de crioconservación y disminuir la temperatura para obtener células crioconservadas, descongelar las células crioconservadas; y reagrupar las células obtenidas después de descongelar en células endocrinas.
MX2020002421A 2017-09-11 2018-09-11 Enriquecimiento de celulas que coexpresan nkx6.1 y peptido c derivadas in vitro a partir de celulas madre. MX2020002421A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17190412 2017-09-11
PCT/EP2018/074390 WO2019048690A1 (en) 2017-09-11 2018-09-11 ENRICHMENT OF CELLS COEXPRESSING NKX6.1 AND PEPTIDE C, IN VITRO DERIVATIVES FROM STEM CELLS

Publications (1)

Publication Number Publication Date
MX2020002421A true MX2020002421A (es) 2020-07-13

Family

ID=59846503

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020002421A MX2020002421A (es) 2017-09-11 2018-09-11 Enriquecimiento de celulas que coexpresan nkx6.1 y peptido c derivadas in vitro a partir de celulas madre.

Country Status (15)

Country Link
US (1) US20200199540A1 (es)
EP (1) EP3681992A1 (es)
JP (1) JP7389020B2 (es)
KR (1) KR20200051664A (es)
CN (1) CN111108190A (es)
AU (1) AU2018330499A1 (es)
BR (1) BR112020004428A2 (es)
CA (1) CA3074910A1 (es)
CO (1) CO2020003122A2 (es)
IL (1) IL272734A (es)
MA (1) MA50279A (es)
MX (1) MX2020002421A (es)
RU (1) RU2020111055A (es)
SG (1) SG11202001906PA (es)
WO (1) WO2019048690A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3953451A1 (en) 2019-04-08 2022-02-16 Novo Nordisk A/S Generation of pancreatic endoderm from stem cell derived definitive endoderm
US20220389378A1 (en) 2019-11-22 2022-12-08 Novo Nordisk A/S Spin-aggregated neural microspheres and the application thereof
WO2023203208A1 (en) 2022-04-21 2023-10-26 Evotec International Gmbh New cell populations and means and methods for their differentiation and preservation
WO2024008810A1 (en) 2022-07-06 2024-01-11 Novo Nordisk A/S Differentiation of stem cells to pancreatic endocrine cells

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2018638C (en) * 1990-06-08 2002-10-01 Wah Jun Tze Banking of pancreatic endocrine cells for transplantation
US20050095703A1 (en) 2001-12-28 2005-05-05 Henrik Semb Method for the establishment of a pluripotent human blastocyst - derived stem cell line
CA2624728A1 (en) 2005-10-07 2007-04-19 Cellartis Ab A method for obtaining a xeno-free hbs cell line
US7695965B2 (en) * 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
US9080145B2 (en) * 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
CA2743641C (en) * 2008-11-14 2024-03-26 Viacyte, Inc. Encapsulation of pancreatic cells derived from human pluripotent stem cells
US9969981B2 (en) * 2010-03-01 2018-05-15 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
WO2012175633A1 (en) 2011-06-21 2012-12-27 Novo Nordisk A/S Efficient induction of definitive endoderm from pluripotent stem cells
CN104903440B (zh) 2012-09-03 2018-04-06 诺和诺德股份有限公司 使用小分子从多能干细胞产生胰内胚层
CN105705634A (zh) 2012-12-31 2016-06-22 詹森生物科技公司 用于分化成胰腺内分泌细胞的人多能细胞的悬浮和群集
WO2014138671A2 (en) * 2013-03-08 2014-09-12 Viacyte, Inc. Cryopreservation, hibernation and room temperature storage of encapulated pancreatic endoderm cell aggregates
US8859286B2 (en) * 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
CN105683361B (zh) * 2013-08-30 2021-04-02 诺和诺德股份有限公司 使用小分子从人多能干细胞产生内分泌祖细胞
JP2017112835A (ja) * 2014-04-17 2017-06-29 東京エレクトロン株式会社 多能性幹細胞の凍結保存方法および凍結保存システム
WO2017094001A1 (en) * 2015-11-30 2017-06-08 Kadimastem Ltd Methods for differentiating and purifying pancreatic endocrine cells
ES2967942T3 (es) 2016-02-24 2024-05-06 Novo Nordisk As Generación de células beta funcionales a partir de progenitoras endocrinas derivadas de células madre pluripotentes humanas

Also Published As

Publication number Publication date
WO2019048690A1 (en) 2019-03-14
BR112020004428A2 (pt) 2020-09-08
CA3074910A1 (en) 2019-03-14
IL272734A (en) 2020-04-30
CO2020003122A2 (es) 2020-06-19
JP2020532978A (ja) 2020-11-19
CN111108190A (zh) 2020-05-05
MA50279A (fr) 2020-07-22
RU2020111055A (ru) 2021-09-17
AU2018330499A1 (en) 2020-04-09
KR20200051664A (ko) 2020-05-13
SG11202001906PA (en) 2020-04-29
US20200199540A1 (en) 2020-06-25
JP7389020B2 (ja) 2023-11-29
EP3681992A1 (en) 2020-07-22
RU2020111055A3 (es) 2022-04-22

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