CO2020003122A2 - Enriquecimiento de células que coexpresan nkx6.1 y péptido c derivadas in vitro a partir de células madre - Google Patents
Enriquecimiento de células que coexpresan nkx6.1 y péptido c derivadas in vitro a partir de células madreInfo
- Publication number
- CO2020003122A2 CO2020003122A2 CONC2020/0003122A CO2020003122A CO2020003122A2 CO 2020003122 A2 CO2020003122 A2 CO 2020003122A2 CO 2020003122 A CO2020003122 A CO 2020003122A CO 2020003122 A2 CO2020003122 A2 CO 2020003122A2
- Authority
- CO
- Colombia
- Prior art keywords
- cells
- vitro
- peptide
- express
- derived
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
- C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/39—Pancreas; Islets of Langerhans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2523/00—Culture process characterised by temperature
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Diabetes (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Developmental Biology & Embryology (AREA)
- Physiology (AREA)
- Nutrition Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Emergency Medicine (AREA)
- Endocrinology (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
La presente invención se relaciona con un método para enriquecer agrupaciones de células que coexpresan NKX6.1 y péptido C derivadas in vitro a partir de células madre, dicho método comprende las etapas de disociar las agrupaciones de células endocrinas en células individuales, tratar las células individuales con medio de crioconservación y disminuir la temperatura para obtener células crioconservadas, descongelar las células crioconservadas; y reagrupar las células obtenidas después de descongelar en células endocrinas.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP17190412 | 2017-09-11 | ||
PCT/EP2018/074390 WO2019048690A1 (en) | 2017-09-11 | 2018-09-11 | ENRICHMENT OF CELLS COEXPRESSING NKX6.1 AND PEPTIDE C, IN VITRO DERIVATIVES FROM STEM CELLS |
Publications (1)
Publication Number | Publication Date |
---|---|
CO2020003122A2 true CO2020003122A2 (es) | 2020-06-19 |
Family
ID=59846503
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CONC2020/0003122A CO2020003122A2 (es) | 2017-09-11 | 2020-03-16 | Enriquecimiento de células que coexpresan nkx6.1 y péptido c derivadas in vitro a partir de células madre |
Country Status (15)
Country | Link |
---|---|
US (1) | US20200199540A1 (es) |
EP (1) | EP3681992A1 (es) |
JP (1) | JP7389020B2 (es) |
KR (1) | KR20200051664A (es) |
CN (1) | CN111108190A (es) |
AU (1) | AU2018330499A1 (es) |
BR (1) | BR112020004428A2 (es) |
CA (1) | CA3074910A1 (es) |
CO (1) | CO2020003122A2 (es) |
IL (1) | IL272734A (es) |
MA (1) | MA50279A (es) |
MX (1) | MX2020002421A (es) |
RU (1) | RU2020111055A (es) |
SG (1) | SG11202001906PA (es) |
WO (1) | WO2019048690A1 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3953451A1 (en) | 2019-04-08 | 2022-02-16 | Novo Nordisk A/S | Generation of pancreatic endoderm from stem cell derived definitive endoderm |
US20220389378A1 (en) | 2019-11-22 | 2022-12-08 | Novo Nordisk A/S | Spin-aggregated neural microspheres and the application thereof |
WO2023203208A1 (en) | 2022-04-21 | 2023-10-26 | Evotec International Gmbh | New cell populations and means and methods for their differentiation and preservation |
WO2024008810A1 (en) | 2022-07-06 | 2024-01-11 | Novo Nordisk A/S | Differentiation of stem cells to pancreatic endocrine cells |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2018638C (en) * | 1990-06-08 | 2002-10-01 | Wah Jun Tze | Banking of pancreatic endocrine cells for transplantation |
US20050095703A1 (en) | 2001-12-28 | 2005-05-05 | Henrik Semb | Method for the establishment of a pluripotent human blastocyst - derived stem cell line |
CA2624728A1 (en) | 2005-10-07 | 2007-04-19 | Cellartis Ab | A method for obtaining a xeno-free hbs cell line |
US7695965B2 (en) * | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
US9080145B2 (en) * | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
CA2743641C (en) * | 2008-11-14 | 2024-03-26 | Viacyte, Inc. | Encapsulation of pancreatic cells derived from human pluripotent stem cells |
US9969981B2 (en) * | 2010-03-01 | 2018-05-15 | Janssen Biotech, Inc. | Methods for purifying cells derived from pluripotent stem cells |
WO2012175633A1 (en) | 2011-06-21 | 2012-12-27 | Novo Nordisk A/S | Efficient induction of definitive endoderm from pluripotent stem cells |
CN104903440B (zh) | 2012-09-03 | 2018-04-06 | 诺和诺德股份有限公司 | 使用小分子从多能干细胞产生胰内胚层 |
CN105705634A (zh) | 2012-12-31 | 2016-06-22 | 詹森生物科技公司 | 用于分化成胰腺内分泌细胞的人多能细胞的悬浮和群集 |
WO2014138671A2 (en) * | 2013-03-08 | 2014-09-12 | Viacyte, Inc. | Cryopreservation, hibernation and room temperature storage of encapulated pancreatic endoderm cell aggregates |
US8859286B2 (en) * | 2013-03-14 | 2014-10-14 | Viacyte, Inc. | In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells |
CN105683361B (zh) * | 2013-08-30 | 2021-04-02 | 诺和诺德股份有限公司 | 使用小分子从人多能干细胞产生内分泌祖细胞 |
JP2017112835A (ja) * | 2014-04-17 | 2017-06-29 | 東京エレクトロン株式会社 | 多能性幹細胞の凍結保存方法および凍結保存システム |
WO2017094001A1 (en) * | 2015-11-30 | 2017-06-08 | Kadimastem Ltd | Methods for differentiating and purifying pancreatic endocrine cells |
ES2967942T3 (es) | 2016-02-24 | 2024-05-06 | Novo Nordisk As | Generación de células beta funcionales a partir de progenitoras endocrinas derivadas de células madre pluripotentes humanas |
-
2018
- 2018-09-11 RU RU2020111055A patent/RU2020111055A/ru unknown
- 2018-09-11 MA MA050279A patent/MA50279A/fr unknown
- 2018-09-11 EP EP18769975.6A patent/EP3681992A1/en active Pending
- 2018-09-11 JP JP2020512652A patent/JP7389020B2/ja active Active
- 2018-09-11 AU AU2018330499A patent/AU2018330499A1/en active Pending
- 2018-09-11 WO PCT/EP2018/074390 patent/WO2019048690A1/en active Application Filing
- 2018-09-11 MX MX2020002421A patent/MX2020002421A/es unknown
- 2018-09-11 US US16/645,840 patent/US20200199540A1/en active Pending
- 2018-09-11 CA CA3074910A patent/CA3074910A1/en active Pending
- 2018-09-11 SG SG11202001906PA patent/SG11202001906PA/en unknown
- 2018-09-11 BR BR112020004428-8A patent/BR112020004428A2/pt unknown
- 2018-09-11 CN CN201880058738.XA patent/CN111108190A/zh active Pending
- 2018-09-11 KR KR1020207008866A patent/KR20200051664A/ko not_active Application Discontinuation
-
2020
- 2020-02-18 IL IL272734A patent/IL272734A/en unknown
- 2020-03-16 CO CONC2020/0003122A patent/CO2020003122A2/es unknown
Also Published As
Publication number | Publication date |
---|---|
WO2019048690A1 (en) | 2019-03-14 |
BR112020004428A2 (pt) | 2020-09-08 |
CA3074910A1 (en) | 2019-03-14 |
IL272734A (en) | 2020-04-30 |
MX2020002421A (es) | 2020-07-13 |
JP2020532978A (ja) | 2020-11-19 |
CN111108190A (zh) | 2020-05-05 |
MA50279A (fr) | 2020-07-22 |
RU2020111055A (ru) | 2021-09-17 |
AU2018330499A1 (en) | 2020-04-09 |
KR20200051664A (ko) | 2020-05-13 |
SG11202001906PA (en) | 2020-04-29 |
US20200199540A1 (en) | 2020-06-25 |
JP7389020B2 (ja) | 2023-11-29 |
EP3681992A1 (en) | 2020-07-22 |
RU2020111055A3 (es) | 2022-04-22 |
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