SG10201803131TA - Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells - Google Patents

Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells

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Publication number
SG10201803131TA
SG10201803131TA SG10201803131TA SG10201803131TA SG10201803131TA SG 10201803131T A SG10201803131T A SG 10201803131TA SG 10201803131T A SG10201803131T A SG 10201803131TA SG 10201803131T A SG10201803131T A SG 10201803131TA SG 10201803131T A SG10201803131T A SG 10201803131TA
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Singapore
Prior art keywords
cell population
cholangiocytes
hepatocyte
methods
pluripotent stem
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SG10201803131TA
Inventor
Gordon Keller
Shinichiro Ogawa
Anand Ghanekar
Christine Bear
Binita M Kamath
Mina Ogawa
James Surapisitchat
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Univ Health Network
Hospital For Sick Children
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Application filed by Univ Health Network, Hospital For Sick Children filed Critical Univ Health Network
Publication of SG10201803131TA publication Critical patent/SG10201803131TA/en

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Abstract

METHODS FOR GENERATING HEPATOCYTES AND CHOLANGIOCYTES FROM PLURIPOTENT STEM CELLS Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates . [Fig. 11]
SG10201803131TA 2013-02-18 2014-02-18 Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells SG10201803131TA (en)

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US (2) US20160002595A1 (en)
EP (1) EP2956540A4 (en)
JP (3) JP2016517266A (en)
KR (5) KR20220000930A (en)
CN (2) CN105121632B (en)
AU (1) AU2014218290B2 (en)
CA (1) CA2901377C (en)
HK (1) HK1218137A1 (en)
IL (2) IL240616B (en)
SG (2) SG11201506520TA (en)
WO (1) WO2014124527A1 (en)
ZA (1) ZA201506189B (en)

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