SG11201805846TA - Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound - Google Patents
Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compoundInfo
- Publication number
- SG11201805846TA SG11201805846TA SG11201805846TA SG11201805846TA SG11201805846TA SG 11201805846T A SG11201805846T A SG 11201805846TA SG 11201805846T A SG11201805846T A SG 11201805846TA SG 11201805846T A SG11201805846T A SG 11201805846TA SG 11201805846T A SG11201805846T A SG 11201805846TA
- Authority
- SG
- Singapore
- Prior art keywords
- cells
- hepatic
- molecular
- low
- weight compound
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/407—Liver; Hepatocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5067—Liver cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases (EC 2.)
- C12N2501/727—Kinases (EC 2.7.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/04—Screening or testing on artificial tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Physiology (AREA)
- Nutrition Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Abstract
The present invention provides a method for producing hepatic stem/progenitor cells from mammal hepatocytes, comprising bringing a TGFI-receptor inhibitor, and optionally a GSK3 inhibitor and/or a ROCK inhibitor into contact with the hepatocytes in vitro. 36
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2016003088 | 2016-01-08 | ||
PCT/JP2017/000342 WO2017119512A1 (en) | 2016-01-08 | 2017-01-06 | Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201805846TA true SG11201805846TA (en) | 2018-08-30 |
Family
ID=59273713
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201805846TA SG11201805846TA (en) | 2016-01-08 | 2017-01-06 | Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound |
SG10202006104YA SG10202006104YA (en) | 2016-01-08 | 2017-01-06 | Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG10202006104YA SG10202006104YA (en) | 2016-01-08 | 2017-01-06 | Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound |
Country Status (13)
Country | Link |
---|---|
US (1) | US10961507B2 (en) |
EP (1) | EP3401392B1 (en) |
JP (1) | JP7063624B2 (en) |
KR (1) | KR20180114032A (en) |
CN (1) | CN108779439B (en) |
AU (1) | AU2017205801A1 (en) |
CA (1) | CA3010808A1 (en) |
DK (1) | DK3401392T3 (en) |
ES (1) | ES2908707T3 (en) |
PL (1) | PL3401392T3 (en) |
RU (1) | RU2018128598A (en) |
SG (2) | SG11201805846TA (en) |
WO (1) | WO2017119512A1 (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20190302100A1 (en) * | 2016-10-28 | 2019-10-03 | National Cancer Center | Method for preparing liver progenitor cells |
WO2018097127A1 (en) * | 2016-11-22 | 2018-05-31 | 国立大学法人京都大学 | Method for stepwise induction from hepatoblasts into biliary epithelial progenitor cells |
CA3116184A1 (en) * | 2018-10-15 | 2020-04-23 | Cynity Co., Ltd. | Method for producing stem/precursor cells, by using low molecular weight compound, from cells derived from endodermal tissue or organ |
JP7284985B2 (en) * | 2019-03-29 | 2023-06-01 | 国立大学法人 長崎大学 | Method for producing cell population containing hepatic progenitor cells |
CN110013544B (en) * | 2019-04-23 | 2021-04-30 | 中国人民解放军第二军医大学 | Application of small molecule combination in preparing medicine for treating chronic liver injury |
EP4379045A1 (en) | 2021-07-29 | 2024-06-05 | Tokyo Medical University | Method for producing highly proliferative cell, and highly proliferative cell and use thereof |
KR20240099268A (en) | 2021-10-18 | 2024-06-28 | 에비아 라이프 사이언시스 인코포레이션 | Composition and method of use in the treatment of liver fibrosis |
AU2022369459A1 (en) | 2021-10-22 | 2024-05-30 | Evia Life Sciences Inc. | Methods for making extracellular vesicles, and compositions and methods of use thereof |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08163996A (en) * | 1994-12-13 | 1996-06-25 | Sumitomo Bakelite Co Ltd | Testing method of toxicity of animal tissue |
JPH08308562A (en) * | 1995-05-16 | 1996-11-26 | Sumitomo Bakelite Co Ltd | Animal cell incubator and measurement of metabolic activity of medicine using the same |
JP2000189189A (en) * | 1998-12-24 | 2000-07-11 | Jms Co Ltd | Method and apparatus for evaluation test using cell |
JP2004198150A (en) * | 2002-12-17 | 2004-07-15 | Banyu Pharmaceut Co Ltd | Method for estimating hepatic metabolic speed of medical substance and method for estimating interaction of medical substance |
JP2006254896A (en) * | 2005-01-07 | 2006-09-28 | Effector Cell Institute Inc | Human hepatocyte-like cell and uses thereof |
PL1969118T5 (en) * | 2005-12-21 | 2015-06-30 | Univ Catholique Louvain | Isolated liver stem cells |
CA2634909A1 (en) * | 2005-12-22 | 2007-07-05 | Vesta Therapeutics, Inc. | Method of using hepatic progenitors in treating liver dysfunction |
WO2009117439A2 (en) * | 2008-03-17 | 2009-09-24 | The Scripps Research Institute | Combined chemical and genetic approaches for generation of induced pluripotent stem cells |
US20120021519A1 (en) | 2008-09-19 | 2012-01-26 | Presidents And Fellows Of Harvard College | Efficient induction of pluripotent stem cells using small molecule compounds |
WO2010093655A2 (en) * | 2009-02-10 | 2010-08-19 | University Of Dayton | Enhanced method for producing stem-like cells from somatic cells |
CN102741394B (en) | 2009-10-16 | 2016-05-04 | 斯克里普斯研究所 | The induction of pluripotent cell |
JP5812492B2 (en) * | 2010-02-03 | 2015-11-11 | 国立研究開発法人国立がん研究センター | Induced hepatic stem cell, method for producing the same, and application of the cell |
CN103380212B (en) * | 2010-12-22 | 2017-04-05 | 菲特治疗公司 | For unicellular sorting and the cell culture platform of enhanced IP SC reprogramming |
US20130071931A1 (en) | 2011-08-02 | 2013-03-21 | National Cancer Center | Process for hepatic differentiation from induced hepatic stem cells, and induced hepatic progenitor cells differentiated thereby |
WO2014025046A1 (en) * | 2012-08-09 | 2014-02-13 | 独立行政法人国立がん研究センター | Promoter of differentiation from hepatic progenitor cell into hepatic cell, and use thereof |
JP6113999B2 (en) * | 2012-10-18 | 2017-04-12 | 株式会社クラレ | Compound screening method |
WO2014083132A1 (en) * | 2012-11-29 | 2014-06-05 | Cellectis Sa | Maturation of hepatocyte-like cells derived from human pluripotent stem cells |
WO2014058080A1 (en) | 2012-11-30 | 2014-04-17 | 独立行政法人理化学研究所 | Method for promoting somatic cell reprogramming, and cell preparation kit |
AU2014314152B2 (en) | 2013-08-28 | 2020-02-06 | Promethera Therapeutics Sa | Method for producing adult liver progenitor cells |
CN105154386B (en) * | 2014-05-30 | 2018-04-24 | 中国人民解放军第二军医大学东方肝胆外科医院 | Human liver cell maintains for a long time and the special culture media and cultural method of propagation secondary culture |
-
2017
- 2017-01-06 RU RU2018128598A patent/RU2018128598A/en not_active Application Discontinuation
- 2017-01-06 EP EP17736049.2A patent/EP3401392B1/en active Active
- 2017-01-06 SG SG11201805846TA patent/SG11201805846TA/en unknown
- 2017-01-06 KR KR1020187022775A patent/KR20180114032A/en active Search and Examination
- 2017-01-06 WO PCT/JP2017/000342 patent/WO2017119512A1/en active Application Filing
- 2017-01-06 PL PL17736049T patent/PL3401392T3/en unknown
- 2017-01-06 JP JP2017560449A patent/JP7063624B2/en active Active
- 2017-01-06 SG SG10202006104YA patent/SG10202006104YA/en unknown
- 2017-01-06 US US16/068,607 patent/US10961507B2/en active Active
- 2017-01-06 CN CN201780005945.4A patent/CN108779439B/en active Active
- 2017-01-06 CA CA3010808A patent/CA3010808A1/en active Pending
- 2017-01-06 AU AU2017205801A patent/AU2017205801A1/en not_active Abandoned
- 2017-01-06 DK DK17736049.2T patent/DK3401392T3/en active
- 2017-01-06 ES ES17736049T patent/ES2908707T3/en active Active
Also Published As
Publication number | Publication date |
---|---|
AU2017205801A1 (en) | 2018-08-16 |
JPWO2017119512A1 (en) | 2018-11-15 |
US20190010464A1 (en) | 2019-01-10 |
WO2017119512A1 (en) | 2017-07-13 |
SG10202006104YA (en) | 2020-07-29 |
EP3401392A4 (en) | 2019-09-18 |
JP7063624B2 (en) | 2022-05-09 |
PL3401392T3 (en) | 2022-06-27 |
KR20180114032A (en) | 2018-10-17 |
DK3401392T3 (en) | 2022-03-07 |
ES2908707T3 (en) | 2022-05-03 |
US10961507B2 (en) | 2021-03-30 |
CA3010808A1 (en) | 2017-07-13 |
EP3401392A1 (en) | 2018-11-14 |
CN108779439A (en) | 2018-11-09 |
RU2018128598A (en) | 2020-02-11 |
CN108779439B (en) | 2023-05-30 |
EP3401392B1 (en) | 2022-02-23 |
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