AR090970A1 - Diferenciacion de celulas madre humanas embrionarias en endodermo pancreatico - Google Patents

Diferenciacion de celulas madre humanas embrionarias en endodermo pancreatico

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Publication number
AR090970A1
AR090970A1 ARP130101563A ARP130101563A AR090970A1 AR 090970 A1 AR090970 A1 AR 090970A1 AR P130101563 A ARP130101563 A AR P130101563A AR P130101563 A ARP130101563 A AR P130101563A AR 090970 A1 AR090970 A1 AR 090970A1
Authority
AR
Argentina
Prior art keywords
cells
pancreatic endoderm
differentiation
embryous
markers indicative
Prior art date
Application number
ARP130101563A
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English (en)
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Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of AR090970A1 publication Critical patent/AR090970A1/es

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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/065—Modulators of histone acetylation
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/19—Growth and differentiation factors [GDF]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
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    • C12N2511/00—Cells for large scale production
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente acta proporciona métodos para promover la diferenciación de células madres pluripotenciales. Particularmente, se proporciona métodos para producir una población de células del endodermo pancreático, en donde la densidad de siembra inicial de pluripotenciales no diferenciadas está definida. Reivindicación 31: Un método para obtener células que expresan marcadores indicativos del endodermo pancreático; el método comprende: a) sembrar las células madre pluripotenciales en una superficie; b) diferenciar las células madre pluripotenciales en células que expresan marcadores indicativos del endodermo definitivo; y c) diferenciar las células que expresan marcadores indicativos del endodermo definitivo en células que expresan marcadores indicativos del endodermo pancreático. Reivindicación 32: El método de acuerdo con la reivindicación 31, en donde las células madre pluripotenciales se siembran en una superficie a una densidad de siembra de aproximadamente 0,8 x 10⁵ células/cm² a aproximadamente 3,0 x 10⁵ células/cm².
ARP130101563A 2012-05-07 2013-05-07 Diferenciacion de celulas madre humanas embrionarias en endodermo pancreatico AR090970A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261643684P 2012-05-07 2012-05-07

Publications (1)

Publication Number Publication Date
AR090970A1 true AR090970A1 (es) 2014-12-17

Family

ID=49551213

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130101563A AR090970A1 (es) 2012-05-07 2013-05-07 Diferenciacion de celulas madre humanas embrionarias en endodermo pancreatico

Country Status (17)

Country Link
US (1) US20140162359A1 (es)
EP (1) EP2847319A4 (es)
JP (1) JP6450674B2 (es)
KR (1) KR20150014478A (es)
CN (1) CN104284977A (es)
AR (1) AR090970A1 (es)
AU (1) AU2013259706A1 (es)
BR (1) BR112014027783A2 (es)
CA (1) CA2872770A1 (es)
HK (1) HK1207885A1 (es)
IL (1) IL235132A0 (es)
IN (1) IN2014DN08561A (es)
MX (1) MX2014013524A (es)
PH (1) PH12014502686A1 (es)
RU (1) RU2668814C2 (es)
SG (1) SG11201406884SA (es)
WO (1) WO2013169769A1 (es)

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US10190096B2 (en) 2014-12-18 2019-01-29 President And Fellows Of Harvard College Methods for generating stem cell-derived β cells and uses thereof
US10443042B2 (en) 2014-12-18 2019-10-15 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
WO2016100930A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Methods for generating stem cell-derived b cells and methods of use thereof
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JP2022534545A (ja) 2019-05-31 2022-08-01 ダブリュ．エル．ゴア アンド アソシエイツ，インコーポレイティド 生体適合性メンブレン複合体
KR20220052370A (ko) 2019-09-05 2022-04-27 크리스퍼 테라퓨틱스 아게 보편적 공여자 세포
WO2021044377A1 (en) 2019-09-05 2021-03-11 Crispr Therapeutics Ag Universal donor cells
CA3203392A1 (en) 2020-12-31 2022-07-07 Alireza Rezania Universal donor cells
CN113046306B (zh) * 2021-03-12 2022-10-18 广东东阳光药业有限公司 一种多能干细胞的培养方法

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Also Published As

Publication number Publication date
RU2014149185A (ru) 2016-06-27
JP6450674B2 (ja) 2019-01-09
SG11201406884SA (en) 2014-11-27
WO2013169769A1 (en) 2013-11-14
RU2668814C2 (ru) 2018-10-02
EP2847319A1 (en) 2015-03-18
US20140162359A1 (en) 2014-06-12
BR112014027783A2 (pt) 2017-06-27
CN104284977A (zh) 2015-01-14
CA2872770A1 (en) 2013-11-14
KR20150014478A (ko) 2015-02-06
HK1207885A1 (en) 2016-02-12
IN2014DN08561A (es) 2015-05-22
IL235132A0 (en) 2014-12-31
AU2013259706A1 (en) 2014-10-30
EP2847319A4 (en) 2015-12-16
PH12014502686A1 (en) 2015-01-26
JP2015516161A (ja) 2015-06-11
MX2014013524A (es) 2015-02-10

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