DK2970899T3 - In vitro-differentiering af pluripotente stamceller i pankreatiske endodermceller (pec) og endokrine celler - Google Patents

In vitro-differentiering af pluripotente stamceller i pankreatiske endodermceller (pec) og endokrine celler Download PDF

Info

Publication number
DK2970899T3
DK2970899T3 DK14721621.2T DK14721621T DK2970899T3 DK 2970899 T3 DK2970899 T3 DK 2970899T3 DK 14721621 T DK14721621 T DK 14721621T DK 2970899 T3 DK2970899 T3 DK 2970899T3
Authority
DK
Denmark
Prior art keywords
cells
pec
pluripotent stem
pancreatic endoderm
vitro differentiation
Prior art date
Application number
DK14721621.2T
Other languages
English (en)
Inventor
Alan Agulnick
Kevin D'amour
Original Assignee
Viacyte Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Viacyte Inc filed Critical Viacyte Inc
Application granted granted Critical
Publication of DK2970899T3 publication Critical patent/DK2970899T3/da

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37Digestive system
    • A61K35/39Pancreas; Islets of Langerhans
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48Reproductive organs
    • A61K35/54Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • A61P3/08Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012Cell encapsulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0676Pancreatic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0676Pancreatic cells
    • C12N5/0678Stem cells; Progenitor cells; Precursor cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/117Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/119Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/15Transforming growth factor beta (TGF-β)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/155Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/16Activin; Inhibin; Mullerian inhibiting substance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/195Heregulin, neu differentiation factor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones
    • C12N2501/38Hormones with nuclear receptors
    • C12N2501/385Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/415Wnt; Frizzeled
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • C12N2501/72Transferases (EC 2.)
    • C12N2501/727Kinases (EC 2.7.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998Proteins not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999Small molecules not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Reproductive Health (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • Nutrition Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physiology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Emergency Medicine (AREA)
  • Endocrinology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK14721621.2T 2013-03-14 2014-03-13 In vitro-differentiering af pluripotente stamceller i pankreatiske endodermceller (pec) og endokrine celler DK2970899T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361781005P 2013-03-14 2013-03-14
US14/106,330 US8859286B2 (en) 2013-03-14 2013-12-13 In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
PCT/US2014/026529 WO2014160413A1 (en) 2013-03-14 2014-03-13 In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (pec) and endocrine cells

Publications (1)

Publication Number Publication Date
DK2970899T3 true DK2970899T3 (da) 2019-08-12

Family

ID=51527928

Family Applications (1)

Application Number Title Priority Date Filing Date
DK14721621.2T DK2970899T3 (da) 2013-03-14 2014-03-13 In vitro-differentiering af pluripotente stamceller i pankreatiske endodermceller (pec) og endokrine celler

Country Status (12)

Country Link
US (5) US8859286B2 (da)
EP (2) EP3521418A1 (da)
JP (6) JP6219494B2 (da)
KR (6) KR102328311B1 (da)
AU (6) AU2014243791B2 (da)
CA (2) CA3204013A1 (da)
DK (1) DK2970899T3 (da)
ES (1) ES2739082T3 (da)
IL (3) IL294691A (da)
RU (3) RU2734657C2 (da)
SG (2) SG10201610787XA (da)
WO (1) WO2014160413A1 (da)

Families Citing this family (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
EP2185693B1 (en) 2007-07-31 2019-07-03 Lifescan, Inc. Differentiation of human embryonic stem cells
CA2959401C (en) 2008-02-21 2021-12-07 Centocor Ortho Biotech Inc. Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
EP2310492B1 (en) 2008-06-30 2015-07-22 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
CA2742268C (en) 2008-10-31 2020-02-18 Centocor Ortho Biotech Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
AU2009316583B2 (en) 2008-11-20 2016-04-21 Janssen Biotech, Inc. Methods and compositions for cell attachment and cultivation on planar substrates
RU2555538C2 (ru) 2008-11-20 2015-07-10 Сентокор Орто Байотек Инк. Культура плюрипотентных стволовых клеток на микроносителях
BR112012001480A2 (pt) 2009-07-20 2015-09-01 Janssen Biotech Inc Diferenciação de células-tronco embriônicas humanas
US9150833B2 (en) 2009-12-23 2015-10-06 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
WO2011109279A2 (en) 2010-03-01 2011-09-09 Centocor Ortho Biotech Inc. Methods for purifying cells derived from pluripotent stem cells
PL2611909T3 (pl) 2010-08-31 2018-05-30 Janssen Biotech, Inc Zróżnicowanie ludzkich embrionalnych komórek macierzystych
BR112014015417A8 (pt) 2011-12-22 2017-07-04 Janssen Biotech Inc diferenciação de células-tronco embrionárias humanas em células positivas de insulina hormonal únicas
US10376845B2 (en) 2016-04-14 2019-08-13 Lockheed Martin Corporation Membranes with tunable selectivity
US10653824B2 (en) 2012-05-25 2020-05-19 Lockheed Martin Corporation Two-dimensional materials and uses thereof
CN104334719B (zh) 2012-06-08 2018-02-13 詹森生物科技公司 人胚胎干细胞向胰腺内分泌细胞的分化
USD933584S1 (en) 2012-11-08 2021-10-19 Sunpower Corporation Solar panel
USD1009775S1 (en) 2014-10-15 2024-01-02 Maxeon Solar Pte. Ltd. Solar panel
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
MX2015008578A (es) 2012-12-31 2015-09-07 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas en celulas endocrinas pancreaticas mediante el uso de relugadores de hb9.
MX2015008619A (es) 2012-12-31 2016-01-12 Janssen Biotech Inc Suspension y agrupamiento de celulas humanas pluripotentes para la diferenciacion a celulas endocrinas pancreaticas.
RU2018116647A (ru) 2012-12-31 2018-10-24 Янссен Байотек, Инк. Культивация эмбриональных стволовых клеток человека в воздушно-жидкостной зоне взаимодействия с целью их дифференцировки в панкреатические эндокринные клетки
WO2014164621A1 (en) 2013-03-12 2014-10-09 Lockheed Martin Corporation Method for forming filter with uniform aperture size
CN106414718A (zh) 2013-06-11 2017-02-15 哈佛学院校长同事会 SC-β细胞以及用于产生其的组合物和方法
US9572918B2 (en) 2013-06-21 2017-02-21 Lockheed Martin Corporation Graphene-based filter for isolating a substance from blood
CN106029596A (zh) 2014-01-31 2016-10-12 洛克希德马丁公司 采用多孔非牺牲性支撑层的二维材料形成复合结构的方法
RU2694311C2 (ru) 2014-05-16 2019-07-11 Янссен Байотек, Инк. Применение малых молекул для увеличения экспрессии mafa в панкреатических эндокринных клетках
US10472610B2 (en) 2014-05-21 2019-11-12 Kyoto University Method for generating pancreatic bud cells and therapeutic agent for pancreatic disease containing pancreatic bud cells
CA2973472A1 (en) 2014-09-02 2016-03-10 Lockheed Martin Corporation Hemodialysis and hemofiltration membranes based upon a two-dimensional membrane material and methods employing same
SG11201701844YA (en) * 2014-10-08 2017-04-27 Agency Science Tech & Res Methods of differentiating stem cells into liver cell lineages
USD896747S1 (en) 2014-10-15 2020-09-22 Sunpower Corporation Solar panel
USD933585S1 (en) 2014-10-15 2021-10-19 Sunpower Corporation Solar panel
USD913210S1 (en) 2014-10-15 2021-03-16 Sunpower Corporation Solar panel
USD999723S1 (en) 2014-10-15 2023-09-26 Sunpower Corporation Solar panel
US11584916B2 (en) 2014-10-17 2023-02-21 Children's Hospital Medical Center Method of making in vivo human small intestine organoids from pluripotent stem cells
WO2016100898A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof
WO2016100909A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College METHODS FOR GENERATING STEM CELL-DERIVED β CELLS AND USES THEREOF
WO2016100930A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Methods for generating stem cell-derived b cells and methods of use thereof
EP3234109A4 (en) 2014-12-19 2018-06-27 Janssen Biotech, Inc. Suspension culturing of pluripotent stem cells
JPWO2016104541A1 (ja) * 2014-12-24 2017-10-05 国立大学法人京都大学 内胚葉系細胞の製造方法、肝臓細胞の製造方法、膵臓細胞の製造方法、内胚葉系細胞の誘導促進剤、肝臓細胞の誘導促進キット、膵臓細胞の誘導促進キット、およびマイクロ流体デバイス
CN107530379A (zh) 2015-03-11 2018-01-02 佩特尼资源有限公司 用于治疗肥胖症和2型糖尿病(t2d)的胰腺内分泌祖细胞疗法
AU2016243750B2 (en) 2015-04-03 2022-02-03 Propagenix Inc. Ex vivo proliferation of epithelial cells
EP3081638A1 (en) 2015-04-16 2016-10-19 Kyoto University Method for producing pseudo-islets
EP3081633A1 (en) 2015-04-16 2016-10-19 ARKRAY, Inc. Device and system for cell culture
PT3286300T (pt) 2015-04-24 2020-12-28 Univ Copenhagen Isolamento de células progenitoras pancreáticas genuínas
AU2016303048A1 (en) 2015-08-05 2018-03-01 Lockheed Martin Corporation Perforatable sheets of graphene-based material
JP2018530499A (ja) 2015-08-06 2018-10-18 ロッキード・マーチン・コーポレーション グラフェンのナノ粒子変性及び穿孔
WO2017043647A1 (ja) * 2015-09-11 2017-03-16 国立研究開発法人理化学研究所 転写因子結合部位特異的なdna脱メチル化方法
EP3892717A1 (en) * 2015-09-11 2021-10-13 Propagenix Inc. Ex vivo proliferation of epithelial cells
US10370637B2 (en) 2015-12-15 2019-08-06 The Regents Of The University Of California Technology for sustaining pluripotency and improved growth of stem cells in culture
WO2017180141A1 (en) 2016-04-14 2017-10-19 Lockheed Martin Corporation Selective interfacial mitigation of graphene defects
MA45479A (fr) 2016-04-14 2019-02-20 Janssen Biotech Inc Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen
CA3020686A1 (en) 2016-04-14 2017-10-19 Lockheed Martin Corporation Method for treating graphene sheets for large-scale transfer using free-float method
KR20190018410A (ko) 2016-04-14 2019-02-22 록히드 마틴 코포레이션 흐름 통로들을 갖는 2차원 막 구조들
KR20180133430A (ko) 2016-04-14 2018-12-14 록히드 마틴 코포레이션 결함 형성 또는 힐링의 인 시츄 모니터링 및 제어를 위한 방법
USD824042S1 (en) 2016-11-10 2018-07-24 Viacyte, Inc. Perforated cell encapsulation device
EP3537986A4 (en) 2016-11-10 2020-06-03 ViaCyte, Inc. PANCREATIC ENDODERM CELLS PDX1 IN CELL DELIVERY DEVICES AND RELATED METHODS
WO2018094107A1 (en) * 2016-11-17 2018-05-24 Baylor College Of Medicine Recreation of pancreatic niche allows for novel methods for human, mature beta derivation from pluripotent stem cells
SG10202105768WA (en) 2016-12-05 2021-06-29 Childrens Hospital Med Ct Colonic organoids and methods of making and using same
SG11201906225PA (en) * 2017-01-17 2019-08-27 Agency Science Tech & Res Maintenance and expansion of pancreatic progenitor cells
US20200080058A1 (en) * 2017-03-21 2020-03-12 Etablissement Francais Du Sang Methods of improving hematopoietic grafts
US10767164B2 (en) 2017-03-30 2020-09-08 The Research Foundation For The State University Of New York Microenvironments for self-assembly of islet organoids from stem cells differentiation
JP7284948B2 (ja) 2017-06-14 2023-06-01 バーテックス ファーマシューティカルズ インコーポレイテッド 療法薬を送達するためのデバイスおよび方法
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
EP3654996A4 (en) * 2017-07-21 2021-03-24 Semma Therapeutics, Inc. REAGGREGATION OF PANCREAS BETA CELLS FROM STEM CELLS
MA50279A (fr) * 2017-09-11 2020-07-22 Novo Nordisk As Enrichissement de cellules coexprimant nkx6.1 et le peptide c, dérivées in vitro à partir de cellules souches
CA2983845C (en) 2017-10-26 2024-01-30 University Of Copenhagen Generation of glucose-responsive beta cells
CA3081762A1 (en) * 2017-11-15 2019-05-23 Semma Therapeutics, Inc. Islet cell manufacturing compositions and methods of use
US11530211B2 (en) 2018-01-18 2022-12-20 Daiichi Sankyo Company, Limited Dihydroindolizinone derivative
JP7248986B2 (ja) * 2018-01-30 2023-03-30 株式会社片岡製作所 インスリン産生細胞の製造方法
EP3758719A4 (en) * 2018-03-02 2021-12-08 Vertex Pharmaceuticals Inc. METHODS FOR IMPROVING THE DIFFERENTIATION OF STEM CELLS INTO BETA CELLS
CA3108275A1 (en) 2018-08-10 2020-02-13 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
US20200080107A1 (en) 2018-09-07 2020-03-12 Crispr Therapeutics Ag Universal donor cells
JP2022534545A (ja) 2019-05-31 2022-08-01 ダブリュ.エル.ゴア アンド アソシエイツ,インコーポレイティド 生体適合性メンブレン複合体
CA3139591C (en) 2019-05-31 2024-01-16 Timothy M. BRUHN A biocompatible membrane composite
WO2020243668A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. Cell encapsulation devices with controlled oxygen diffusion distances
EP3975926A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates, Inc. A biocompatible membrane composite
WO2021030373A1 (en) * 2019-08-13 2021-02-18 Children's Hospital Medical Center Improved methods for making organoid compositions
CN114364791A (zh) 2019-09-05 2022-04-15 克里斯珀医疗股份公司 通用供体细胞
US11104918B2 (en) 2019-09-05 2021-08-31 Crispr Therapeutics Ag Universal donor cells
CN114945378A (zh) * 2019-10-10 2022-08-26 休斯敦大学体系 用于分层上皮干细胞的基于饲养层和无饲养层干细胞培养系统及其相关用途
CN113637630B (zh) * 2020-05-11 2024-03-29 中国科学院分子细胞科学卓越创新中心 一种胰岛样细胞团及其制法和应用
AU2021319209A1 (en) 2020-07-31 2023-03-02 Vertex Pharmaceuticals Incorporated Differentiation of pancreatic endocrine cells
EP4204542A1 (en) * 2020-08-28 2023-07-05 Novo Nordisk A/S Method for screening in vitro population of stem cell derived beta like cells and novel markers thereof
CA3203392A1 (en) 2020-12-31 2022-07-07 Alireza Rezania Universal donor cells
CN114350598A (zh) * 2021-12-03 2022-04-15 深圳华源再生医学有限公司 一种多能干细胞体外形成3d球的培养方法
WO2023201361A1 (en) 2022-04-15 2023-10-19 Aspen Neuroscience, Inc. Methods of classifying the differentiation state of cells and related compositions of differentiated cells
WO2023203208A1 (en) 2022-04-21 2023-10-26 Evotec International Gmbh New cell populations and means and methods for their differentiation and preservation
WO2024020587A2 (en) 2022-07-22 2024-01-25 Tome Biosciences, Inc. Pleiopluripotent stem cell programmable gene insertion

Family Cites Families (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US800807A (en) 1905-04-03 1905-10-03 Hans P Larsen Composition of matter for soldering.
US2161804A (en) 1935-11-09 1939-06-13 Baldwin Southwark Corp Hydraulic press
US5529914A (en) 1990-10-15 1996-06-25 The Board Of Regents The Univeristy Of Texas System Gels for encapsulation of biological materials
KR940702451A (ko) 1991-09-06 1994-08-20 고야 다다시 4-아미로(알킬)시클로헥산-1-카르복사미드 화합물 및 그 용도
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5690926A (en) 1992-10-08 1997-11-25 Vanderbilt University Pluripotential embryonic cells and methods of making same
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US20030059913A1 (en) 1995-11-20 2003-03-27 Walter Weyler Novel Microorganisms with ability to degrade indole and novel enzymes therefrom
CA2247502A1 (en) 1996-02-28 1997-09-04 Vanderbilt University Compositions and methods of making embryonic stem cells
US6075007A (en) 1997-07-17 2000-06-13 Regeneron Pharmaceuticals, Inc. Modified noggin polypeptide and compositions
CA2307807C (en) 1997-10-23 2008-09-02 Andrea G. Bodnar Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6756511B2 (en) 2000-01-24 2004-06-29 Merck Sharp & Dohme Limited Gamma-secretase inhibitors
GB0005251D0 (en) 2000-03-03 2000-04-26 Merck Sharp & Dohme Therapeutic compounds
US7005252B1 (en) 2000-03-09 2006-02-28 Wisconsin Alumni Research Foundation Serum free cultivation of primate embryonic stem cells
GB0008710D0 (en) 2000-04-07 2000-05-31 Merck Sharp & Dohme Therapeutic compounds
ES2248397T3 (es) 2000-11-02 2006-03-16 MERCK SHARP & DOHME LTD. Sulfamidas como inhibidores de la gamma-secretasa.
JP2002184539A (ja) 2000-12-14 2002-06-28 Auto Network Gijutsu Kenkyusho:Kk コネクタ
TWI336328B (en) 2001-03-23 2011-01-21 Bayer Healthcare Llc Rho-kinase inhibitors
HN2002000067A (es) 2001-03-23 2003-10-24 Bayer Healthcare Llc Inhibidores de la rho - quinasa.
AU2002319780A1 (en) 2001-08-06 2003-02-24 Bresagen, Ltd. Alternative compositions and methods for the culture of stem cells
JP2003097552A (ja) 2001-09-21 2003-04-03 Nsk Ltd 摩擦付加装置および直動案内装置
US20030062225A1 (en) 2001-10-03 2003-04-03 Stucky Paul A. Elevator load bearing assembly having a detectable element that is indicative of local strain
US20030077256A1 (en) * 2001-10-19 2003-04-24 Paul Czernichow Pancreas regeneration using embryonic pancreatic cells
CA2692325C (en) 2001-12-07 2015-10-20 Geron Corporation Islet cells from human embryonic stem cells
DE10297545T5 (de) 2001-12-13 2005-01-13 General Binding Corp., Northbrook Transportsystem für Blattübergabe zwischen Blatt- oder Blattstapel verarbeitender Ausrüstung
CA2472619A1 (en) 2002-01-10 2003-07-24 Bayer Corporation Fused pyrimidine derivates as rho-kinase inhibitors
WO2003062227A1 (en) 2002-01-23 2003-07-31 Bayer Pharmaceuticals Corporation Rho-kinase inhibitors
US6924290B2 (en) 2002-01-23 2005-08-02 Bayer Pharmaceuticals Corporation Rho-kinase inhibitors
US20040039796A1 (en) 2002-08-08 2004-02-26 Virtual Radio, Inc. Personalized cyber disk jockey and Internet radio advertising
GB0223038D0 (en) 2002-10-04 2002-11-13 Merck Sharp & Dohme Therapeutic compounds
GB0223039D0 (en) 2002-10-04 2002-11-13 Merck Sharp & Dohme Therapeutic compounds
CA2501949A1 (en) 2002-10-11 2004-04-22 Novocell, Inc. Implantation of encapsulated biological materials for treating diseases
WO2004039796A1 (de) 2002-10-28 2004-05-13 Bayer Healthcare Ag Heteroaryloxy-substituierte phenylaminopyrimidine als rho-kinaseinhibitoren
GB0225474D0 (en) 2002-11-01 2002-12-11 Merck Sharp & Dohme Therapeutic agents
GB0326039D0 (en) 2003-11-07 2003-12-10 Merck Sharp & Dohme Therapeutic agents
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
AU2004309421B2 (en) 2003-12-23 2011-04-21 Viacyte, Inc. Definitive endoderm
WO2005080598A1 (ja) 2004-02-19 2005-09-01 Dainippon Sumitomo Pharma Co., Ltd. 体細胞核初期化物質のスクリーニング方法
US8597947B2 (en) * 2004-12-29 2013-12-03 Hadasit Medical Research Services & Development Limited Undifferentiated stem cell culture systems
US7739764B2 (en) 2005-04-27 2010-06-22 Whirlpool Corporation Method and apparatus for monitoring load size and load imbalance in washing machine
DK2674485T3 (da) 2005-10-27 2019-08-26 Viacyte Inc Pdx-1 udtrykkende dorsal og ventral fortarm endoderm
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
CA2643478C (en) 2006-02-23 2019-06-18 Novocell, Inc. Compositions and methods useful for culturing differentiable cells
CA2644468C (en) 2006-03-02 2022-02-01 Cythera, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
WO2007139929A2 (en) 2006-05-25 2007-12-06 The Burnham Institute For Medical Research Methods for culture and production of single cell populations of human embryonic stem cells
US20090298169A1 (en) * 2006-06-02 2009-12-03 The University Of Georgia Research Foundation Pancreatic and Liver Endoderm Cells and Tissue by Differentiation of Definitive Endoderm Cells Obtained from Human Embryonic Stems
WO2008013664A2 (en) 2006-07-26 2008-01-31 Cythera, Inc. Methods of producing pancreatic hormones
DK177279B1 (da) 2006-07-27 2012-09-24 Vegano Aps Ukrudtsmiddel samt fremgangsmåde til fremstilling
CN101743306A (zh) 2007-03-23 2010-06-16 威斯康星校友研究基金会 体细胞重编程
CN105176919A (zh) * 2007-07-18 2015-12-23 生命扫描有限公司 人胚胎干细胞的分化
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
US8623650B2 (en) 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
RU2391401C2 (ru) * 2008-02-12 2010-06-10 Общество с ограниченной ответственностью "Лаборатория клеточного мониторинга" СПОСОБ ПОЛУЧЕНИЯ ПОПУЛЯЦИИ CD4+CD25+Foxp3+ Т-ЛИМФОЦИТОВ ЧЕЛОВЕКА ex vivo, СПОСОБ ЛЕЧЕНИЯ ЗАБОЛЕВАНИЯ
CA2718904C (en) 2008-03-17 2017-01-03 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
RU2359030C1 (ru) * 2008-03-19 2009-06-20 Общество С Ограниченной Ответственностью "Лаборатория Клеточных Технологий" Способ получения эндотелиальных клеток из эмбриональных стволовых клеток человека (варианты)
US8338170B2 (en) 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
WO2009154606A1 (en) 2008-06-03 2009-12-23 Cythera, Inc. Growth factors for production of definitive endoderm
US20090298178A1 (en) 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm
EP3447128A1 (en) 2008-06-04 2019-02-27 FUJIFILM Cellular Dynamics, Inc. Methods for the production of ips cells using non-viral approach
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
CA2742583C (en) 2008-11-04 2022-09-27 Viacyte, Inc. Stem cell aggregate suspension compositions and methods for differentiation thereof
ES2932850T3 (es) 2008-11-14 2023-01-27 Viacyte Inc Encapsulación de células pancreáticas derivadas de células madre pluripotentes humanas
JP5488000B2 (ja) 2009-02-02 2014-05-14 リコーイメージング株式会社 高変倍ズームレンズ系
DK2421957T3 (da) * 2009-04-22 2021-01-25 Viacyte Inc Cellesammensætninger afledt af dedifferentierede omprogrammerede celler
US9328331B2 (en) * 2009-05-21 2016-05-03 Joslin Diabetes Center, Inc. Compositions and methods for promoting beta cell maturity
CN102482640B (zh) * 2009-07-20 2015-03-11 詹森生物科技公司 人胚胎干细胞的分化
RU2664864C1 (ru) 2009-12-23 2018-08-23 Янссен Байотек, Инк. Способы увеличения экспрессии ngn3 и nkx6.1 в эндокринных клетках поджелудочной железы
US9150833B2 (en) 2009-12-23 2015-10-06 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
KR101827956B1 (ko) 2009-12-29 2018-02-12 포리 오토메이션, 인코포레이티드 무인 반송차용 구동 기구
US8424928B2 (en) 2010-09-24 2013-04-23 Thase Enterprise Co., Ltd. Door handle having a handgrip changeable indoor and outdoor
US20120270890A1 (en) 2011-04-22 2012-10-25 Cheng-Ho Chung Methods of Pancreatic Beta Cell Regeneration
BR112014015417A8 (pt) 2011-12-22 2017-07-04 Janssen Biotech Inc diferenciação de células-tronco embrionárias humanas em células positivas de insulina hormonal únicas
CN104334719B (zh) 2012-06-08 2018-02-13 詹森生物科技公司 人胚胎干细胞向胰腺内分泌细胞的分化
MX352528B (es) 2012-10-01 2017-11-29 Nissan Motor Dispositivo de control de estabilidad.
RU2018116647A (ru) * 2012-12-31 2018-10-24 Янссен Байотек, Инк. Культивация эмбриональных стволовых клеток человека в воздушно-жидкостной зоне взаимодействия с целью их дифференцировки в панкреатические эндокринные клетки
DE102013212018A1 (de) 2013-06-25 2015-01-08 Evonik Industries Ag Metalloxid-Prekursoren, sie enthaltende Beschichtungszusammensetzungen, und ihre Verwendung
US10702008B2 (en) 2018-02-26 2020-07-07 Hbn Shoe, Llc Device and method of constructing shoes
EP3986392A4 (en) 2019-06-20 2023-07-12 ChemoCentryx, Inc. COMPOUNDS FOR TREATMENT OF PD-L1 DISEASES
US11586805B2 (en) 2021-07-26 2023-02-21 Atlassian Pty Ltd. Machine-learning-based natural language processing techniques for low-latency document summarization

Also Published As

Publication number Publication date
AU2019264658A1 (en) 2019-12-05
IL240028A0 (en) 2015-09-24
SG10201610787XA (en) 2017-02-27
JP2018007670A (ja) 2018-01-18
US20230165908A1 (en) 2023-06-01
RU2764465C1 (ru) 2022-01-17
AU2021202943A1 (en) 2021-06-03
KR102288191B1 (ko) 2021-08-11
AU2014243791A1 (en) 2015-07-30
RU2734657C2 (ru) 2020-10-21
KR20210100743A (ko) 2021-08-17
AU2019202849A1 (en) 2019-05-16
KR20200015855A (ko) 2020-02-12
AU2019202849B2 (en) 2019-08-22
US20140271566A1 (en) 2014-09-18
JP2018183142A (ja) 2018-11-22
KR102077367B1 (ko) 2020-02-13
IL240028B (en) 2021-05-31
CA3204013A1 (en) 2014-10-02
JP2022017361A (ja) 2022-01-25
AU2021202943B2 (en) 2021-08-19
JP6654664B2 (ja) 2020-02-26
CA2897984C (en) 2023-08-22
IL283094A (en) 2021-06-30
KR20200138434A (ko) 2020-12-09
US8859286B2 (en) 2014-10-14
AU2021266324C1 (en) 2023-06-08
EP3521418A1 (en) 2019-08-07
US20150017135A1 (en) 2015-01-15
EP2970899B1 (en) 2019-05-08
JP6219494B2 (ja) 2017-10-25
ES2739082T3 (es) 2020-01-28
JP7187647B2 (ja) 2022-12-12
AU2014243791B2 (en) 2019-01-31
KR20150127063A (ko) 2015-11-16
KR20210142763A (ko) 2021-11-25
AU2021266324A1 (en) 2021-12-09
JP2016513972A (ja) 2016-05-19
WO2014160413A1 (en) 2014-10-02
AU2019264658B2 (en) 2021-02-18
RU2018134644A (ru) 2019-03-21
KR20210034100A (ko) 2021-03-29
US10376545B2 (en) 2019-08-13
AU2021266324B2 (en) 2022-12-22
CA2897984A1 (en) 2014-10-02
US20170202885A1 (en) 2017-07-20
KR102328311B1 (ko) 2021-11-19
JP2023029922A (ja) 2023-03-07
AU2023201602A1 (en) 2023-04-13
IL294691A (en) 2022-09-01
RU2670064C2 (ru) 2018-10-17
SG11201505866RA (en) 2015-08-28
RU2018134644A3 (da) 2019-03-21
US11446335B2 (en) 2022-09-20
KR102187510B1 (ko) 2020-12-08
EP2970899A1 (en) 2016-01-20
JP6966583B2 (ja) 2021-11-17
JP2020096605A (ja) 2020-06-25
US20190307810A1 (en) 2019-10-10
US9650610B2 (en) 2017-05-16
AU2021202943C1 (en) 2022-02-24
JP6355802B2 (ja) 2018-07-11
RU2015137400A (ru) 2017-04-18
JP7490035B2 (ja) 2024-05-24
KR102231393B1 (ko) 2021-03-25

Similar Documents

Publication Publication Date Title
DK2970899T3 (da) In vitro-differentiering af pluripotente stamceller i pankreatiske endodermceller (pec) og endokrine celler
EP3334820C0 (en) EXPANDED AND INDUCED PLURIPOTENT STEM CELLS, METHODS OF MANUFACTURE AND USE
HK1250740A1 (zh) 補充牛磺酸的細胞培養基和使用方法
IL241930B (en) Isolated naive pluripotent stem cells and methods of generating same
DK3060652T3 (da) In vitro-produktion af fortarm-stamceller
HK1217450A1 (zh) 為移植物之器官移植忍耐性的器官及造血細胞合併
HK1224335A1 (zh) 使用合成信使 無飼養層衍生人誘導多能幹細胞
BR112015030946A2 (pt) composição compreendendo uma célula ß pancreática não-nativa, método de diferenciação de células progenitoras em células ß pancreáticas in vitro e uso de uma célula ß pancreática não-nativa
SG11201508387WA (en) Polycaprolactone microcarriers for stem cell culture and fabrication thereof
SG11201603047PA (en) Suspension and clustering of human pluripotent stem cells for differentiation into pancreatic endocrine cells
DK3059307T4 (da) Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier
SG11201505965TA (en) Culture method for stable undifferentiated proliferation of pluripotent stem cells
DK3017038T3 (da) Differentiering af pluripotente stamceller og hjerteprogenitorceller i tværstribede kardiomyocytfibre under anvendelse af lamininer ln-511, ln-521 og ln-221
IL247798B (en) Expansion of mature stem cells in vitro
DK3344758T3 (da) Fremgangsmåde in vitro til differentiering af en human pluripotent stamcellepopulation i en kardiomyocytcellepopulation
EP2949746A4 (en) METHOD OF SUB-CULTURE OF PLURIPOTENT STEM CELLS
EP3070159A4 (en) METHOD AND PLANT FOR CURING PLURIPOTENT STEM CELLS
HK1221258A1 (zh) 用於體外培養幹細胞的方法和培養基
DK3060653T3 (da) Fremgangsmåde til reduktion af den inflammatoriske aktivitet i et stamcelletransplantat og anvendelse deraf
EP3045530A4 (en) PROCESS FOR CULTURING PLURIPOTENT STEM CELLS AND KIT AND CULTURE MEDIA THEREOF
TH1601002463A (th) การแขวนลอยและการคลัสเตอร์เซลล์ต้นกำเนิดพลูริโพเทนต์ของมนุษย์ สำหรับการเจริญเปลี่ยนรูปไปเป็นเซลล์ต่อมไร้ท่อของตับอ่อน
TH1401006654A (th) การเจริญเปลี่ยนรูปของเซลล์ต้นกำเนิดจากเอ็มบริโอของมนุษย์ไปเป็นเอนโดเดิร์มของตับอ่อน