DK3059307T4 - Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier - Google Patents

Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier Download PDF

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Publication number
DK3059307T4
DK3059307T4 DK15305265.9T DK15305265T DK3059307T4 DK 3059307 T4 DK3059307 T4 DK 3059307T4 DK 15305265 T DK15305265 T DK 15305265T DK 3059307 T4 DK3059307 T4 DK 3059307T4
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laminin
differentiation
cell lines
pluripotent cells
hepatocyte cell
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DK15305265.9T
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DK3059307T5 (da
DK3059307T3 (da
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Angélique Fourrier
Tuan Huy Nguyen
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Inst Nat Sante Rech Med
Univ Nantes
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
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  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK15305265.9T 2015-02-20 2015-02-20 Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier DK3059307T5 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP15305265.9A EP3059307B2 (en) 2015-02-20 2015-02-20 Use of a laminin for differentiating pluripotent cells into hepatocyte lineage cells

Publications (3)

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DK3059307T3 DK3059307T3 (da) 2019-01-14
DK3059307T4 true DK3059307T4 (da) 2022-08-01
DK3059307T5 DK3059307T5 (da) 2022-08-29

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DK15305265.9T DK3059307T5 (da) 2015-02-20 2015-02-20 Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier
DK16705202.6T DK3259345T3 (da) 2015-02-20 2016-02-19 Anvendelse af laminin til differentiering af pluripotente celler i hepatocyt-linie-celler

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US (2) US20180030415A1 (da)
EP (2) EP3059307B2 (da)
JP (2) JP6840084B2 (da)
CN (2) CN107532144A (da)
CA (1) CA2986830A1 (da)
CY (1) CY1121243T1 (da)
DK (2) DK3059307T5 (da)
ES (2) ES2703167T5 (da)
FI (1) FI3259345T3 (da)
HR (2) HRP20182119T4 (da)
HU (2) HUE041518T2 (da)
LT (2) LT3059307T (da)
PL (2) PL3059307T5 (da)
PT (2) PT3059307T (da)
RS (2) RS58070B2 (da)
SI (2) SI3059307T2 (da)
WO (1) WO2016131959A1 (da)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2872620T (pt) * 2012-07-12 2020-03-03 Isletone Ab Derivação e autorrenovação de células lsl+ e usos das mesmas
HUE041518T2 (hu) * 2015-02-20 2019-05-28 Inst Nat Sante Rech Med Laminin alkalmazása pluripotens sejtek hepatocita sejtvonalhoz tartozó sejtekké történõ differenciálódásához
US10683486B2 (en) 2015-10-30 2020-06-16 Biolamina Ab Methods for producing hepatocytes
WO2018019780A1 (en) * 2016-07-28 2018-02-01 F. Hoffmann-La Roche Ag Non-human primate induced pluripotent stem cell derived hepatocytes and uses thereof
RU2758006C2 (ru) * 2016-11-16 2021-10-25 Сината Терапьютикс Лимитед Анализ плюрипотентных стволовых клеток
US11530388B2 (en) 2017-02-14 2022-12-20 University of Pittsburgh—of the Commonwealth System of Higher Education Methods of engineering human induced pluripotent stem cells to produce liver tissue
JP6950887B2 (ja) * 2017-04-28 2021-10-13 米満　吉和 体外型の人工肝臓、及び体外型の人工肝臓用又は肝細胞培養用の器具
EP3619293B1 (en) 2017-05-05 2023-11-29 National University of Singapore Methods for culturing human keratinocytes
JP2021525100A (ja) * 2018-05-16 2021-09-24 インセルム（インスティチュート ナショナル デ ラ サンテ エ デ ラ リシェルシェ メディカル） 増殖性肝細胞を培養する方法
WO2020006539A1 (en) * 2018-06-29 2020-01-02 Platelet Biogenesis, Inc. Compositions for drug delivery and methods of use thereof
US11227435B2 (en) 2018-08-13 2022-01-18 Magic Leap, Inc. Cross reality system
CN117802031A (zh) * 2018-09-30 2024-04-02 中国科学院分子细胞科学卓越创新中心 肝细胞的体外扩增培养方法及应用
US20210395679A1 (en) * 2018-11-09 2021-12-23 Children's Hospital Medical Center In vitro cell culture system for producing hepatocyte-like cells and uses thereof
CN111621467A (zh) * 2020-06-12 2020-09-04 青岛大学 一种诱导人脂肪间充质干细胞分化为肝脏样细胞的方法
CN115851578B (zh) * 2022-12-23 2023-11-21 华南理工大学 一种3d悬浮诱导持续扩增肝祖细胞类器官和/或肝细胞类器官的试剂盒及其应用

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US7332336B2 (en) * 2003-08-19 2008-02-19 Effector Cell Institute, Inc. Methods for inducing differentiation of pluripotent cells
JP2005095138A (ja) * 2003-08-19 2005-04-14 Effector Cell Institute Inc 多分化能を有する細胞の分化誘導方法
WO2006040763A2 (en) 2004-10-12 2006-04-20 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
AU2006325975B2 (en) 2005-12-13 2011-12-08 Kyoto University Nuclear reprogramming factor
US8741643B2 (en) * 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US8951799B2 (en) * 2007-01-04 2015-02-10 Biolamina Ab Composition and method for enabling proliferation of pluripotent stem cells
WO2009013254A1 (en) * 2007-07-20 2009-01-29 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts stem cells
WO2009057831A1 (ja) 2007-10-31 2009-05-07 Kyoto University 核初期化方法
EP2268796A1 (en) 2008-03-17 2011-01-05 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Vectors and methods for generating vector-free induced pluripotent stem (ips) cells using site-specific recombination
WO2010042490A1 (en) 2008-10-06 2010-04-15 Boston Medical Center Corporation A single lentiviral vector system for induced pluripotent (ips) stem cells derivation
JP2011010653A (ja) * 2009-06-05 2011-01-20 Kumamoto Univ 細胞の分化誘導方法
CN102666853B (zh) * 2009-10-28 2014-12-03 财团法人日本健康科学振兴财团 由干细胞向肝细胞的分化诱导方法
AU2011263652B2 (en) * 2010-06-11 2014-11-20 Takara Bio Europe Ab 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes
KR101921350B1 (ko) * 2010-12-17 2018-11-22 바이오라미나 에이비 인간 다능성 줄기세포를 유지하기 위한 시스템
US10240124B2 (en) * 2011-09-22 2019-03-26 Biolamina Ab Cell culture substrate comprising a laminin and a cadherin
US20130330825A1 (en) * 2012-06-07 2013-12-12 City Of Hope Attachment substrates for directed differentiation of human embryonic stem cells in culture
CN104884468B (zh) * 2012-12-28 2018-08-31 国立大学法人大阪大学 附加有胶原结合性分子的改造层粘连蛋白及其利用
CN113913366A (zh) * 2013-02-18 2022-01-11 大学健康网络 由多能干细胞生成肝细胞和胆管细胞的方法
JP6421335B2 (ja) * 2013-04-08 2018-11-14 国立研究開発法人医薬基盤・健康・栄養研究所 肝幹前駆様細胞の培養方法及びその培養物
HUE041518T2 (hu) 2015-02-20 2019-05-28 Inst Nat Sante Rech Med Laminin alkalmazása pluripotens sejtek hepatocita sejtvonalhoz tartozó sejtekké történõ differenciálódásához

Also Published As

Publication number Publication date
JP7132463B2 (ja) 2022-09-07
EP3059307B1 (en) 2018-10-17
JP2021006054A (ja) 2021-01-21
DK3059307T5 (da) 2022-08-29
CN111548985A (zh) 2020-08-18
FI3259345T3 (fi) 2023-04-03
DK3059307T3 (da) 2019-01-14
RS58070B1 (sr) 2019-02-28
JP6840084B2 (ja) 2021-03-17
EP3059307A1 (en) 2016-08-24
US20240150721A1 (en) 2024-05-09
ES2940191T3 (es) 2023-05-04
SI3059307T2 (sl) 2022-08-31
ES2703167T3 (es) 2019-03-07
CY1121243T1 (el) 2020-05-29
PT3059307T (pt) 2019-01-23
PL3059307T3 (pl) 2019-05-31
RS58070B2 (sr) 2022-10-31
WO2016131959A1 (en) 2016-08-25
LT3259345T (lt) 2023-03-27
EP3259345B1 (en) 2023-02-08
EP3059307B2 (en) 2022-05-04
HRP20182119T4 (hr) 2022-09-02
HUE041518T2 (hu) 2019-05-28
RS64062B1 (sr) 2023-04-28
HRP20182119T1 (hr) 2019-03-08
EP3259345A1 (en) 2017-12-27
CN107532144A (zh) 2018-01-02
HRP20230302T1 (hr) 2023-05-12
SI3059307T1 (sl) 2019-03-29
LT3059307T (lt) 2019-01-10
US20180030415A1 (en) 2018-02-01
JP2018508207A (ja) 2018-03-29
CA2986830A1 (en) 2016-08-25
HUE061457T2 (hu) 2023-06-28
PT3259345T (pt) 2023-03-15
PL3259345T3 (pl) 2023-05-02
DK3259345T3 (da) 2023-03-27
SI3259345T1 (sl) 2023-08-31
PL3059307T5 (pl) 2023-04-03
ES2703167T5 (es) 2022-09-30

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