DK3059307T5 - Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier - Google Patents
Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier Download PDFInfo
- Publication number
- DK3059307T5 DK3059307T5 DK15305265.9T DK15305265T DK3059307T5 DK 3059307 T5 DK3059307 T5 DK 3059307T5 DK 15305265 T DK15305265 T DK 15305265T DK 3059307 T5 DK3059307 T5 DK 3059307T5
- Authority
- DK
- Denmark
- Prior art keywords
- laminin
- differentiation
- cell lines
- pluripotent cells
- hepatocyte cell
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/98—Xeno-free medium and culture conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/12—Hepatocyte growth factor [HGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/237—Oncostatin M [OSM]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/40—Regulators of development
- C12N2501/415—Wnt; Frizzeled
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases (EC 2.)
- C12N2501/727—Kinases (EC 2.7.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15305265.9A EP3059307B2 (en) | 2015-02-20 | 2015-02-20 | Use of a laminin for differentiating pluripotent cells into hepatocyte lineage cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DK3059307T3 DK3059307T3 (da) | 2019-01-14 |
| DK3059307T4 DK3059307T4 (da) | 2022-08-01 |
| DK3059307T5 true DK3059307T5 (da) | 2022-08-29 |
Family
ID=52598699
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15305265.9T DK3059307T5 (da) | 2015-02-20 | 2015-02-20 | Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier |
| DK16705202.6T DK3259345T3 (da) | 2015-02-20 | 2016-02-19 | Anvendelse af laminin til differentiering af pluripotente celler i hepatocyt-linie-celler |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16705202.6T DK3259345T3 (da) | 2015-02-20 | 2016-02-19 | Anvendelse af laminin til differentiering af pluripotente celler i hepatocyt-linie-celler |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US20180030415A1 (da) |
| EP (2) | EP3059307B2 (da) |
| JP (2) | JP6840084B2 (da) |
| CN (2) | CN107532144A (da) |
| CA (1) | CA2986830A1 (da) |
| CY (1) | CY1121243T1 (da) |
| DK (2) | DK3059307T5 (da) |
| ES (2) | ES2703167T5 (da) |
| FI (1) | FI3259345T3 (da) |
| HR (2) | HRP20182119T4 (da) |
| HU (2) | HUE041518T2 (da) |
| LT (2) | LT3059307T (da) |
| PL (2) | PL3059307T5 (da) |
| PT (2) | PT3059307T (da) |
| RS (2) | RS58070B2 (da) |
| SI (2) | SI3059307T2 (da) |
| WO (1) | WO2016131959A1 (da) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT2872620T (pt) * | 2012-07-12 | 2020-03-03 | Isletone Ab | Derivação e autorrenovação de células lsl+ e usos das mesmas |
| PL3059307T5 (pl) * | 2015-02-20 | 2023-04-03 | Inserm (Institut National De La Santé Et De La Recherche Medicale) | Zastosowanie lamininy do różnicowania komórek pluripotencjalnych w komórki linii hepatocytów |
| AU2016344599B2 (en) * | 2015-10-30 | 2020-12-17 | Biolamina Ab | Methods for producing hepatocytes |
| WO2018019780A1 (en) * | 2016-07-28 | 2018-02-01 | F. Hoffmann-La Roche Ag | Non-human primate induced pluripotent stem cell derived hepatocytes and uses thereof |
| CN110573607B (zh) * | 2016-11-16 | 2023-10-20 | 洋蓟治疗有限公司 | 多能干细胞测定 |
| CA3051442A1 (en) | 2017-02-14 | 2018-08-23 | Alejandro Soto-Gutierrez | Methods of engineering human induced pluripotent stem cells to produce liver tissue |
| JP6950887B2 (ja) * | 2017-04-28 | 2021-10-13 | 米満 吉和 | 体外型の人工肝臓、及び体外型の人工肝臓用又は肝細胞培養用の器具 |
| CA3061599A1 (en) * | 2017-05-05 | 2018-11-08 | National University Of Singapore | Methods for culturing human keratinocytes |
| CN112513252A (zh) * | 2018-05-16 | 2021-03-16 | 国家健康科学研究所 | 培养增殖性肝细胞的方法 |
| WO2020006539A1 (en) * | 2018-06-29 | 2020-01-02 | Platelet Biogenesis, Inc. | Compositions for drug delivery and methods of use thereof |
| US11227435B2 (en) | 2018-08-13 | 2022-01-18 | Magic Leap, Inc. | Cross reality system |
| CN117802031A (zh) * | 2018-09-30 | 2024-04-02 | 中国科学院分子细胞科学卓越创新中心 | 肝细胞的体外扩增培养方法及应用 |
| EP3969568A4 (en) * | 2018-11-09 | 2022-09-07 | Children's Hospital Medical Center | IN VITRO CELL CULTURE SYSTEM FOR THE PRODUCTION OF HEPATOCYTE-LIKE CELLS AND THEIR USES |
| CN111621467A (zh) * | 2020-06-12 | 2020-09-04 | 青岛大学 | 一种诱导人脂肪间充质干细胞分化为肝脏样细胞的方法 |
| CN115851578B (zh) * | 2022-12-23 | 2023-11-21 | 华南理工大学 | 一种3d悬浮诱导持续扩增肝祖细胞类器官和/或肝细胞类器官的试剂盒及其应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| JP2005095138A (ja) * | 2003-08-19 | 2005-04-14 | Effector Cell Institute Inc | 多分化能を有する細胞の分化誘導方法 |
| US7332336B2 (en) * | 2003-08-19 | 2008-02-19 | Effector Cell Institute, Inc. | Methods for inducing differentiation of pluripotent cells |
| WO2006040763A2 (en) | 2004-10-12 | 2006-04-20 | Technion Research & Development Foundation Ltd. | Isolated primate embryonic cells and methods of generating and using same |
| EP4223769A3 (en) | 2005-12-13 | 2023-11-01 | Kyoto University | Nuclear reprogramming factor |
| US8741643B2 (en) * | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
| US8951799B2 (en) * | 2007-01-04 | 2015-02-10 | Biolamina Ab | Composition and method for enabling proliferation of pluripotent stem cells |
| EP2179034A1 (en) * | 2007-07-20 | 2010-04-28 | Cellartis AB | A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts stem cells |
| EP2096169B1 (en) | 2007-10-31 | 2020-11-18 | Kyoto University | Nuclear reprogramming method |
| WO2009115295A1 (en) | 2008-03-17 | 2009-09-24 | Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | Vectors and methods for generating vector-free induced pluripotent stem (ips) cells using site-specific recombination |
| WO2010042490A1 (en) | 2008-10-06 | 2010-04-15 | Boston Medical Center Corporation | A single lentiviral vector system for induced pluripotent (ips) stem cells derivation |
| WO2010140464A1 (ja) * | 2009-06-05 | 2010-12-09 | 国立大学法人 熊本大学 | 細胞の分化誘導方法 |
| CN102666853B (zh) * | 2009-10-28 | 2014-12-03 | 财团法人日本健康科学振兴财团 | 由干细胞向肝细胞的分化诱导方法 |
| EP2580319B1 (en) * | 2010-06-11 | 2016-07-20 | Takara Bio Europe AB | 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes |
| SG191216A1 (en) | 2010-12-17 | 2013-07-31 | Biolamina Ab | Cell culture medium |
| AU2012311233B2 (en) * | 2011-09-22 | 2017-03-16 | Sergey Rodin | Cell culture substrate comprising a laminin and a cadherin |
| US20130330825A1 (en) * | 2012-06-07 | 2013-12-12 | City Of Hope | Attachment substrates for directed differentiation of human embryonic stem cells in culture |
| US10000554B2 (en) * | 2012-12-28 | 2018-06-19 | Osaka University | Modified laminin containing collagen binding molecule and use thereof |
| EP2956540A4 (en) * | 2013-02-18 | 2016-08-03 | Univ Health Network | METHOD OF GENERATING HEPATOCYTES AND CHOLANGIOZYTS FROM PLURIPOTENTIAL STEM CELLS |
| EA201501002A1 (ru) * | 2013-04-08 | 2016-02-29 | Нэшнл Инститьютс Оф Байомедикал Инновейшн, Хелт Энд Ньютришн | Способ культивирования гепатобластподобных клеток и продукт их культивирования |
| PL3059307T5 (pl) * | 2015-02-20 | 2023-04-03 | Inserm (Institut National De La Santé Et De La Recherche Medicale) | Zastosowanie lamininy do różnicowania komórek pluripotencjalnych w komórki linii hepatocytów |
-
2015
- 2015-02-20 PL PL15305265.9T patent/PL3059307T5/pl unknown
- 2015-02-20 LT LTEP15305265.9T patent/LT3059307T/lt unknown
- 2015-02-20 HU HUE15305265A patent/HUE041518T2/hu unknown
- 2015-02-20 RS RS20181530A patent/RS58070B2/sr unknown
- 2015-02-20 EP EP15305265.9A patent/EP3059307B2/en active Active
- 2015-02-20 ES ES15305265T patent/ES2703167T5/es active Active
- 2015-02-20 PT PT15305265T patent/PT3059307T/pt unknown
- 2015-02-20 SI SI201530533T patent/SI3059307T2/sl unknown
- 2015-02-20 HR HRP20182119TT patent/HRP20182119T4/hr unknown
- 2015-02-20 DK DK15305265.9T patent/DK3059307T5/da active
-
2016
- 2016-02-19 PL PL16705202.6T patent/PL3259345T3/pl unknown
- 2016-02-19 US US15/551,551 patent/US20180030415A1/en not_active Abandoned
- 2016-02-19 HU HUE16705202A patent/HUE061457T2/hu unknown
- 2016-02-19 EP EP16705202.6A patent/EP3259345B1/en active Active
- 2016-02-19 DK DK16705202.6T patent/DK3259345T3/da active
- 2016-02-19 RS RS20230211A patent/RS64062B1/sr unknown
- 2016-02-19 WO PCT/EP2016/053560 patent/WO2016131959A1/en active Application Filing
- 2016-02-19 HR HRP20230302TT patent/HRP20230302T1/hr unknown
- 2016-02-19 FI FIEP16705202.6T patent/FI3259345T3/fi active
- 2016-02-19 CN CN201680010458.2A patent/CN107532144A/zh active Pending
- 2016-02-19 LT LTEPPCT/EP2016/053560T patent/LT3259345T/lt unknown
- 2016-02-19 CN CN202010391416.6A patent/CN111548985A/zh active Pending
- 2016-02-19 CA CA2986830A patent/CA2986830A1/en active Pending
- 2016-02-19 PT PT167052026T patent/PT3259345T/pt unknown
- 2016-02-19 SI SI201631673T patent/SI3259345T1/sl unknown
- 2016-02-19 JP JP2017543392A patent/JP6840084B2/ja active Active
- 2016-02-19 ES ES16705202T patent/ES2940191T3/es active Active
-
2018
- 2018-12-14 CY CY20181101344T patent/CY1121243T1/el unknown
-
2020
- 2020-10-01 JP JP2020167137A patent/JP7132463B2/ja active Active
-
2023
- 2023-11-17 US US18/512,545 patent/US20240150721A1/en active Pending
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK3059307T5 (da) | Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier | |
| IL267616A (en) | Immunologically engineered pluripotent cells | |
| IL254071B1 (en) | Mammalian cells are enriched in active mitochondria | |
| DK3224351T3 (da) | Dyrkningsmedium til ekspandering af brystepitelstamceller | |
| DK3207124T3 (da) | Fremgangsmåder og sammensætninger til generering eller bevaring af pluripotente celler | |
| DK3500664T3 (da) | Fremgangsmåde til differentiering af pluripotente celler | |
| IL287553A (en) | Modified pluripotent cells | |
| GB201611982D0 (en) | Cell culture | |
| DK3464550T3 (da) | Beholdere og spinnerkobler med reduceret hjulslør til dyrkning af celler | |
| DK3253863T3 (da) | Bioreaktorsystem til celledyrkning | |
| DK3344758T3 (da) | Fremgangsmåde in vitro til differentiering af en human pluripotent stamcellepopulation i en kardiomyocytcellepopulation | |
| DK3250202T3 (da) | Sammensætning til anvendelse til forøgelse af effektiviteten af inkorporering af hæmatopoetiske stamceller efter transplantation | |
| SG11201706886PA (en) | Cell culture method for mesenchymal stem cells | |
| DK3766582T3 (da) | Cellekultur | |
| DK3365432T3 (en) | Stem cell therapy based on adipose-derived stem cells | |
| DK3304037T3 (da) | Fremgangsmåde til opsamling af cellekolonier | |
| DK3257097T3 (da) | Tætning til fast polymerelektrolytbrændselscelle | |
| DE112017006675A5 (de) | PEM-Brennstoffzelle | |
| FI20145745A (fi) | Elektrolyyttimembraani käytettäväksi sähkökemiallisessa kennossa | |
| ZA201804586B (en) | Fuel cell electrolyte management device | |
| GB201402543D0 (en) | Multipotent stromal cell loaded microcarriers | |
| DK3347487T3 (da) | Ikke-invasive fremgangsmåder til vurdering af genetisk integritet af pluripotente stamceller | |
| DK3194574T3 (da) | Fremgangsmåde til dyrkning af stamceller in vitro | |
| GB2534241B (en) | Non-prismatic electrochemical cell | |
| GB2539233B (en) | Electrochemical cell |