DK3059307T3 - Anvendelse af et laminin til differentiering af pluripotente celler til hepatocyt-cellelinjer - Google Patents
Anvendelse af et laminin til differentiering af pluripotente celler til hepatocyt-cellelinjer Download PDFInfo
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- DK3059307T3 DK3059307T3 DK15305265.9T DK15305265T DK3059307T3 DK 3059307 T3 DK3059307 T3 DK 3059307T3 DK 15305265 T DK15305265 T DK 15305265T DK 3059307 T3 DK3059307 T3 DK 3059307T3
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Claims (11)
1. Anvendelse in vitro af et humant laminin (LN) som en matrix til hepatisk differentiering.
2. Anvendelse in vitro af et humant LN til induktion og/eller forbedret differentiering af en population af pluripotente celler, en population af multipotente celler eller en population af definitive endoderme (DE) celler til en population af hepatocyt-cellelinjer.
3. Anvendelse ifølge krav 1 eller 2, hvor LN er valgt fra gruppen bestående af laminin-111 (LN-111), laminin-211 (LN-211), laminin-332 (LN-332), laminin-411 (LN-411), laminin-421 (LN-421), laminin-511 (LN-511)og laminin-521 (LN-521).
4. Anvendelse ifølge et hvilket som helst af kravene 1 til 3, hvor LN er human rekombinant LN-111 eller human rekombinant LN-521.
5. Fremgangsmåde in vitro til induktion af human hepatisk differentiering, der omfatter følgende trin: (i) dyrkning af en population af humane DE-celler på en bærer coatet med et humant laminin i et hepatisk induktionsmedium for at frembringe en population af humane hepatoblast-lignende celler, og (ii) dyrkning af populationen af humane hepatoblast-lignende celler på en bærer coatet med et humant laminin i et hepatisk modningsmedium for at frembringe en population af humane føtale hepatocyt-lignende celler.
6. Fremgangsmåde in vitro til induktion af human hepatisk differentiering, der omfatter følgende trin: (i) dyrkning af en population af humane pluripotente celler på en bærer coatet med et humant laminin i et endodermt induktionsmedium for at frembringe en population af humane DE-celler, (ii) dyrkning af populationen af humane DE-celler på en bærer coatet med et humant laminin i et hepatisk induktionsmedium for at frembringe en population af humane hepatoblast-lignende celler, og (iii) dyrkning af populationen af humane hepatoblast-lignende celler på en bærer coatet med et humant laminin i et hepatisk modningsmedium for at frembringe en population af humane føtale hepatocyt-lignende celler.
7. Fremgangsmåde in vitro ifølge krav 5 eller 6, hvor det hepatiske induktionsmedium er et kemisk defineret medium, der omfatter knoglemorfogenetisk protein 4 (BMP4) og en fibroblast-vækstfaktor valgt fra FGF10 og FGF2.
8. Fremgangsmåde in vitro ifølge et hvilket som helst af kravene 5 til 7, hvor det hepatiske modningsmedium er et kemisk defineret medium, der omfatter hepatisk vækstfaktor (HGF) og oncostatin M (OSM).
9. Fremgangsmåde in vitro ifølge krav 6, hvor det endoderme induktionsmedium er et kemisk defineret medium, der omfatter mindst Activin A og eventuelt WNT3A.
10. Fremgangsmåde in vitro ifølge et hvilket som helst af kravene 5 til 9, hvor det hepatiske induktionsmedium og/eller det endoderme induktionsmedium endvidere omfatter en ROCK-hæmmer.
11. Fremgangsmåde in vitro ifølge et hvilket som helst af kravene 5 til 10, hvor de humane hepatoblast-lignende celler udtrykker hepatocyt-nuklearfaktor 4-alpha (HNF4a) og ikke eller i alt væsentligt ikke udtrykker alpha-fetoprotein (AFP).
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| EP15305265.9A EP3059307B2 (en) | 2015-02-20 | 2015-02-20 | Use of a laminin for differentiating pluripotent cells into hepatocyte lineage cells |
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| WO2014011095A1 (en) * | 2012-07-12 | 2014-01-16 | Isletone Ab | Derivation and self-renewal of isi1+ cells and uses thereof |
| RS58070B2 (sr) * | 2015-02-20 | 2022-10-31 | Inst Nat Sante Rech Med | Upotreba laminina za diferencijaciju pluripotentnih ćelija u ćelije hepatocitne linije |
| EP3368657B1 (en) | 2015-10-30 | 2021-06-30 | Biolamina AB | Methods for producing hepatocytes |
| JP7209619B2 (ja) * | 2016-07-28 | 2023-01-20 | エフ．ホフマン－ラ ロシュ アーゲー | 非ヒト霊長類人工多能性幹細胞由来肝細胞およびその使用 |
| KR102516844B1 (ko) * | 2016-11-16 | 2023-04-04 | 시나타 세라퓨틱스 엘티디 | 만능성 줄기 세포 분석 |
| WO2018152120A1 (en) | 2017-02-14 | 2018-08-23 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Methods of engineering human induced pluripotent stem cells to produce liver tissue |
| JP6950887B2 (ja) * | 2017-04-28 | 2021-10-13 | 米満 吉和 | 体外型の人工肝臓、及び体外型の人工肝臓用又は肝細胞培養用の器具 |
| AU2018262323A1 (en) | 2017-05-05 | 2019-11-21 | National University Of Singapore | Methods for culturing human keratinocytes |
| BR112020013656A2 (pt) | 2018-01-05 | 2020-12-01 | Platelet Biogenesis, Inc. | composições e métodos para produção de megacariócitos |
| EP3794107A1 (en) * | 2018-05-16 | 2021-03-24 | Institut National de la Santé et de la Recherche Médicale (INSERM) | Method of culturing proliferative hepatocytes |
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| US20210395679A1 (en) * | 2018-11-09 | 2021-12-23 | Children's Hospital Medical Center | In vitro cell culture system for producing hepatocyte-like cells and uses thereof |
| CN111621467A (zh) * | 2020-06-12 | 2020-09-04 | 青岛大学 | 一种诱导人脂肪间充质干细胞分化为肝脏样细胞的方法 |
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| WO2010042490A1 (en) | 2008-10-06 | 2010-04-15 | Boston Medical Center Corporation | A single lentiviral vector system for induced pluripotent (ips) stem cells derivation |
| WO2010140464A1 (ja) * | 2009-06-05 | 2010-12-09 | 国立大学法人 熊本大学 | 細胞の分化誘導方法 |
| JP5745423B2 (ja) * | 2009-10-28 | 2015-07-08 | 公益財団法人ヒューマンサイエンス振興財団 | 幹細胞から肝細胞への分化誘導方法 |
| US9127255B2 (en) * | 2010-06-11 | 2015-09-08 | Takara Bio Europe Ab | 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes |
| WO2012080842A1 (en) | 2010-12-17 | 2012-06-21 | Biolamina Ab | Recombinant laminin-521 |
| US10240124B2 (en) * | 2011-09-22 | 2019-03-26 | Biolamina Ab | Cell culture substrate comprising a laminin and a cadherin |
| US20130330825A1 (en) * | 2012-06-07 | 2013-12-12 | City Of Hope | Attachment substrates for directed differentiation of human embryonic stem cells in culture |
| JP5858411B2 (ja) * | 2012-12-28 | 2016-02-10 | 国立大学法人大阪大学 | コラーゲン結合性分子を付加した改変ラミニンおよびその利用 |
| SG11201506520TA (en) * | 2013-02-18 | 2015-09-29 | Univ Health Network | Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells |
| US20160046904A1 (en) † | 2013-04-08 | 2016-02-18 | National Institutes Of Biomedical Innovation, Health And Nutrition | Method For Culturing Hepatoblast-Like Cells And Culture Product Thereof |
| RS58070B2 (sr) | 2015-02-20 | 2022-10-31 | Inst Nat Sante Rech Med | Upotreba laminina za diferencijaciju pluripotentnih ćelija u ćelije hepatocitne linije |
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