PL3059307T5 - Zastosowanie lamininy do różnicowania komórek pluripotencjalnych w komórki linii hepatocytów - Google Patents

Zastosowanie lamininy do różnicowania komórek pluripotencjalnych w komórki linii hepatocytów

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Publication number
PL3059307T5
PL3059307T5 PL15305265.9T PL15305265T PL3059307T5 PL 3059307 T5 PL3059307 T5 PL 3059307T5 PL 15305265 T PL15305265 T PL 15305265T PL 3059307 T5 PL3059307 T5 PL 3059307T5
Authority
PL
Poland
Prior art keywords
cells
laminin
differentiating pluripotent
hepatocyte lineage
pluripotent cells
Prior art date
Application number
PL15305265.9T
Other languages
English (en)
Other versions
PL3059307T3 (pl
Inventor
Tuan Huy NGUYEN
Angélique Fourrier
Original Assignee
Inserm (Institut National De La Santé Et De La Recherche Medicale)
Nantes Université
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52598699&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL3059307(T5) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Inserm (Institut National De La Santé Et De La Recherche Medicale), Nantes Université filed Critical Inserm (Institut National De La Santé Et De La Recherche Medicale)
Publication of PL3059307T3 publication Critical patent/PL3059307T3/pl
Publication of PL3059307T5 publication Critical patent/PL3059307T5/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
PL15305265.9T 2015-02-20 2015-02-20 Zastosowanie lamininy do różnicowania komórek pluripotencjalnych w komórki linii hepatocytów PL3059307T5 (pl)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP15305265.9A EP3059307B2 (en) 2015-02-20 2015-02-20 Use of a laminin for differentiating pluripotent cells into hepatocyte lineage cells

Publications (2)

Publication Number Publication Date
PL3059307T3 PL3059307T3 (pl) 2019-05-31
PL3059307T5 true PL3059307T5 (pl) 2023-04-03

Family

ID=52598699

Family Applications (2)

Application Number Title Priority Date Filing Date
PL15305265.9T PL3059307T5 (pl) 2015-02-20 2015-02-20 Zastosowanie lamininy do różnicowania komórek pluripotencjalnych w komórki linii hepatocytów
PL16705202.6T PL3259345T3 (pl) 2015-02-20 2016-02-19 Zastosowanie lamininy do różnicowania komórek pluripotencjalnych w komórki linii hepatocytów

Family Applications After (1)

Application Number Title Priority Date Filing Date
PL16705202.6T PL3259345T3 (pl) 2015-02-20 2016-02-19 Zastosowanie lamininy do różnicowania komórek pluripotencjalnych w komórki linii hepatocytów

Country Status (17)

Country Link
US (2) US20180030415A1 (pl)
EP (2) EP3059307B2 (pl)
JP (2) JP6840084B2 (pl)
CN (2) CN107532144A (pl)
CA (1) CA2986830A1 (pl)
CY (1) CY1121243T1 (pl)
DK (2) DK3059307T5 (pl)
ES (2) ES2703167T5 (pl)
FI (1) FI3259345T3 (pl)
HR (2) HRP20182119T4 (pl)
HU (2) HUE041518T2 (pl)
LT (2) LT3059307T (pl)
PL (2) PL3059307T5 (pl)
PT (2) PT3059307T (pl)
RS (2) RS58070B2 (pl)
SI (2) SI3059307T2 (pl)
WO (1) WO2016131959A1 (pl)

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PT2872620T (pt) * 2012-07-12 2020-03-03 Isletone Ab Derivação e autorrenovação de células lsl+ e usos das mesmas
HUE041518T2 (hu) * 2015-02-20 2019-05-28 Inst Nat Sante Rech Med Laminin alkalmazása pluripotens sejtek hepatocita sejtvonalhoz tartozó sejtekké történõ differenciálódásához
US10683486B2 (en) 2015-10-30 2020-06-16 Biolamina Ab Methods for producing hepatocytes
WO2018019780A1 (en) * 2016-07-28 2018-02-01 F. Hoffmann-La Roche Ag Non-human primate induced pluripotent stem cell derived hepatocytes and uses thereof
RU2758006C2 (ru) * 2016-11-16 2021-10-25 Сината Терапьютикс Лимитед Анализ плюрипотентных стволовых клеток
US11530388B2 (en) 2017-02-14 2022-12-20 University of Pittsburgh—of the Commonwealth System of Higher Education Methods of engineering human induced pluripotent stem cells to produce liver tissue
JP6950887B2 (ja) * 2017-04-28 2021-10-13 米満　吉和 体外型の人工肝臓、及び体外型の人工肝臓用又は肝細胞培養用の器具
EP3619293B1 (en) 2017-05-05 2023-11-29 National University of Singapore Methods for culturing human keratinocytes
JP2021525100A (ja) * 2018-05-16 2021-09-24 インセルム（インスティチュート ナショナル デ ラ サンテ エ デ ラ リシェルシェ メディカル） 増殖性肝細胞を培養する方法
WO2020006539A1 (en) * 2018-06-29 2020-01-02 Platelet Biogenesis, Inc. Compositions for drug delivery and methods of use thereof
US11227435B2 (en) 2018-08-13 2022-01-18 Magic Leap, Inc. Cross reality system
CN117802031A (zh) * 2018-09-30 2024-04-02 中国科学院分子细胞科学卓越创新中心 肝细胞的体外扩增培养方法及应用
US20210395679A1 (en) * 2018-11-09 2021-12-23 Children's Hospital Medical Center In vitro cell culture system for producing hepatocyte-like cells and uses thereof
CN111621467A (zh) * 2020-06-12 2020-09-04 青岛大学 一种诱导人脂肪间充质干细胞分化为肝脏样细胞的方法
CN115851578B (zh) * 2022-12-23 2023-11-21 华南理工大学 一种3d悬浮诱导持续扩增肝祖细胞类器官和/或肝细胞类器官的试剂盒及其应用

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US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US7332336B2 (en) * 2003-08-19 2008-02-19 Effector Cell Institute, Inc. Methods for inducing differentiation of pluripotent cells
JP2005095138A (ja) * 2003-08-19 2005-04-14 Effector Cell Institute Inc 多分化能を有する細胞の分化誘導方法
WO2006040763A2 (en) 2004-10-12 2006-04-20 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
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US8741643B2 (en) * 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US8951799B2 (en) * 2007-01-04 2015-02-10 Biolamina Ab Composition and method for enabling proliferation of pluripotent stem cells
WO2009013254A1 (en) * 2007-07-20 2009-01-29 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts stem cells
WO2009057831A1 (ja) 2007-10-31 2009-05-07 Kyoto University 核初期化方法
EP2268796A1 (en) 2008-03-17 2011-01-05 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Vectors and methods for generating vector-free induced pluripotent stem (ips) cells using site-specific recombination
WO2010042490A1 (en) 2008-10-06 2010-04-15 Boston Medical Center Corporation A single lentiviral vector system for induced pluripotent (ips) stem cells derivation
JP2011010653A (ja) * 2009-06-05 2011-01-20 Kumamoto Univ 細胞の分化誘導方法
CN102666853B (zh) * 2009-10-28 2014-12-03 财团法人日本健康科学振兴财团 由干细胞向肝细胞的分化诱导方法
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US10240124B2 (en) * 2011-09-22 2019-03-26 Biolamina Ab Cell culture substrate comprising a laminin and a cadherin
US20130330825A1 (en) * 2012-06-07 2013-12-12 City Of Hope Attachment substrates for directed differentiation of human embryonic stem cells in culture
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CN113913366A (zh) * 2013-02-18 2022-01-11 大学健康网络 由多能干细胞生成肝细胞和胆管细胞的方法
JP6421335B2 (ja) * 2013-04-08 2018-11-14 国立研究開発法人医薬基盤・健康・栄養研究所 肝幹前駆様細胞の培養方法及びその培養物
HUE041518T2 (hu) 2015-02-20 2019-05-28 Inst Nat Sante Rech Med Laminin alkalmazása pluripotens sejtek hepatocita sejtvonalhoz tartozó sejtekké történõ differenciálódásához

Also Published As

Publication number Publication date
JP7132463B2 (ja) 2022-09-07
EP3059307B1 (en) 2018-10-17
JP2021006054A (ja) 2021-01-21
DK3059307T5 (da) 2022-08-29
CN111548985A (zh) 2020-08-18
FI3259345T3 (fi) 2023-04-03
DK3059307T4 (da) 2022-08-01
DK3059307T3 (en) 2019-01-14
RS58070B1 (sr) 2019-02-28
JP6840084B2 (ja) 2021-03-17
EP3059307A1 (en) 2016-08-24
US20240150721A1 (en) 2024-05-09
ES2940191T3 (es) 2023-05-04
SI3059307T2 (sl) 2022-08-31
ES2703167T3 (es) 2019-03-07
CY1121243T1 (el) 2020-05-29
PT3059307T (pt) 2019-01-23
PL3059307T3 (pl) 2019-05-31
RS58070B2 (sr) 2022-10-31
WO2016131959A1 (en) 2016-08-25
LT3259345T (lt) 2023-03-27
EP3259345B1 (en) 2023-02-08
EP3059307B2 (en) 2022-05-04
HRP20182119T4 (hr) 2022-09-02
HUE041518T2 (hu) 2019-05-28
RS64062B1 (sr) 2023-04-28
HRP20182119T1 (hr) 2019-03-08
EP3259345A1 (en) 2017-12-27
CN107532144A (zh) 2018-01-02
HRP20230302T1 (hr) 2023-05-12
SI3059307T1 (sl) 2019-03-29
LT3059307T (lt) 2019-01-10
US20180030415A1 (en) 2018-02-01
JP2018508207A (ja) 2018-03-29
CA2986830A1 (en) 2016-08-25
HUE061457T2 (hu) 2023-06-28
PT3259345T (pt) 2023-03-15
PL3259345T3 (pl) 2023-05-02
DK3259345T3 (da) 2023-03-27
SI3259345T1 (sl) 2023-08-31
ES2703167T5 (es) 2022-09-30

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