LT3259345T - Laminino naudojimas pluripotentinių ląstelių diferenciacijai į hepatocitų linijos ląsteles - Google Patents

Laminino naudojimas pluripotentinių ląstelių diferenciacijai į hepatocitų linijos ląsteles

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Publication number
LT3259345T
LT3259345T LTEPPCT/EP2016/053560T LT16053560T LT3259345T LT 3259345 T LT3259345 T LT 3259345T LT 16053560 T LT16053560 T LT 16053560T LT 3259345 T LT3259345 T LT 3259345T
Authority
LT
Lithuania
Prior art keywords
cells
laminin
differentiating pluripotent
hepatocyte lineage
pluripotent cells
Prior art date
Application number
LTEPPCT/EP2016/053560T
Other languages
English (en)
Inventor
Tuan Huy NGUYEN
Angélique Fourrier
Original Assignee
INSERM (Institut National de la Santé et de la Recherche Médicale)
Nantes Université
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52598699&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=LT3259345(T) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by INSERM (Institut National de la Santé et de la Recherche Médicale), Nantes Université filed Critical INSERM (Institut National de la Santé et de la Recherche Médicale)
Publication of LT3259345T publication Critical patent/LT3259345T/lt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
LTEPPCT/EP2016/053560T 2015-02-20 2016-02-19 Laminino naudojimas pluripotentinių ląstelių diferenciacijai į hepatocitų linijos ląsteles LT3259345T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15305265.9A EP3059307B2 (en) 2015-02-20 2015-02-20 Use of a laminin for differentiating pluripotent cells into hepatocyte lineage cells
PCT/EP2016/053560 WO2016131959A1 (en) 2015-02-20 2016-02-19 Use of a laminin for differentiating pluripotent cells into hepatocyte lineage cells

Publications (1)

Publication Number Publication Date
LT3259345T true LT3259345T (lt) 2023-03-27

Family

ID=52598699

Family Applications (2)

Application Number Title Priority Date Filing Date
LTEP15305265.9T LT3059307T (lt) 2015-02-20 2015-02-20 Laminino panaudojimas pliuripotentinių ląstelių diferenciacijai į hepatocitų linijos ląsteles
LTEPPCT/EP2016/053560T LT3259345T (lt) 2015-02-20 2016-02-19 Laminino naudojimas pluripotentinių ląstelių diferenciacijai į hepatocitų linijos ląsteles

Family Applications Before (1)

Application Number Title Priority Date Filing Date
LTEP15305265.9T LT3059307T (lt) 2015-02-20 2015-02-20 Laminino panaudojimas pliuripotentinių ląstelių diferenciacijai į hepatocitų linijos ląsteles

Country Status (17)

Country Link
US (2) US20180030415A1 (lt)
EP (2) EP3059307B2 (lt)
JP (2) JP6840084B2 (lt)
CN (2) CN107532144A (lt)
CA (1) CA2986830A1 (lt)
CY (1) CY1121243T1 (lt)
DK (2) DK3059307T5 (lt)
ES (2) ES2703167T5 (lt)
FI (1) FI3259345T3 (lt)
HR (2) HRP20182119T4 (lt)
HU (2) HUE041518T2 (lt)
LT (2) LT3059307T (lt)
PL (2) PL3059307T5 (lt)
PT (2) PT3059307T (lt)
RS (2) RS58070B2 (lt)
SI (2) SI3059307T2 (lt)
WO (1) WO2016131959A1 (lt)

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PL2872620T3 (pl) * 2012-07-12 2020-08-24 Isletone Ab Wyprowadzenie i samoodnawianie komórek isl1+ i ich zastosowania
DK3059307T5 (da) * 2015-02-20 2022-08-29 Inst Nat Sante Rech Med Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier
US10683486B2 (en) 2015-10-30 2020-06-16 Biolamina Ab Methods for producing hepatocytes
WO2018019780A1 (en) * 2016-07-28 2018-02-01 F. Hoffmann-La Roche Ag Non-human primate induced pluripotent stem cell derived hepatocytes and uses thereof
SG11201903428XA (en) * 2016-11-16 2019-05-30 Cynata Therapeutics Ltd Pluripotent stem cell assay
EP3583201B1 (en) * 2017-02-14 2024-01-17 University of Pittsburgh - Of the Commonwealth System of Higher Education Methods of engineering human induced pluripotent stem cells to produce liver tissue
JP6950887B2 (ja) * 2017-04-28 2021-10-13 米満　吉和 体外型の人工肝臓、及び体外型の人工肝臓用又は肝細胞培養用の器具
US11655451B2 (en) 2017-05-05 2023-05-23 National University Of Singapore Methods for culturing human keratinocytes
EP3813853A4 (en) * 2018-06-29 2022-04-06 Platelet Biogenesis, Inc. DELIVERY COMPOSITIONS AND METHODS OF USE
US11227435B2 (en) 2018-08-13 2022-01-18 Magic Leap, Inc. Cross reality system
CN117802031A (zh) * 2018-09-30 2024-04-02 中国科学院分子细胞科学卓越创新中心 肝细胞的体外扩增培养方法及应用
WO2020097555A1 (en) * 2018-11-09 2020-05-14 Children's Hospital Medical Center In vitro cell culture system for producing hepatocyte-like cells and uses thereof
CN111621467A (zh) * 2020-06-12 2020-09-04 青岛大学 一种诱导人脂肪间充质干细胞分化为肝脏样细胞的方法
CN115851578B (zh) * 2022-12-23 2023-11-21 华南理工大学 一种3d悬浮诱导持续扩增肝祖细胞类器官和/或肝细胞类器官的试剂盒及其应用

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JP2005095138A (ja) * 2003-08-19 2005-04-14 Effector Cell Institute Inc 多分化能を有する細胞の分化誘導方法
US7332336B2 (en) * 2003-08-19 2008-02-19 Effector Cell Institute, Inc. Methods for inducing differentiation of pluripotent cells
US8354277B2 (en) 2004-10-12 2013-01-15 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells derived from extended blastocysts
EP2208786B1 (en) 2005-12-13 2018-08-01 Kyoto University Nuclear reprogramming factor
US8741643B2 (en) * 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US8951799B2 (en) * 2007-01-04 2015-02-10 Biolamina Ab Composition and method for enabling proliferation of pluripotent stem cells
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JP2011516042A (ja) 2008-03-17 2011-05-26 へルムホルツ・ツェントルム・ミュンヘン−ドイチェス・フォルシュングスツェントルム・ヒューア・ゲズントハイト・ウント・ウムヴェルト（ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング） 部位特異的組み換えを用いて無ベクター誘導多能性幹（ｉＰＳ）細胞を作製するベクターおよびその方法
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WO2014124527A1 (en) * 2013-02-18 2014-08-21 University Health Network Methods for generating hepatocytes and cholangiocytes from pluripotent stem cells
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DK3059307T5 (da) 2015-02-20 2022-08-29 Inst Nat Sante Rech Med Anvendelse af et laminin til differentiering af pluripotente celler i hepatocytcellelinier

Also Published As

Publication number Publication date
SI3059307T2 (sl) 2022-08-31
HRP20182119T1 (hr) 2019-03-08
JP2021006054A (ja) 2021-01-21
EP3059307B2 (en) 2022-05-04
US20180030415A1 (en) 2018-02-01
HUE061457T2 (hu) 2023-06-28
SI3059307T1 (sl) 2019-03-29
PL3059307T3 (pl) 2019-05-31
PL3059307T5 (pl) 2023-04-03
RS58070B1 (sr) 2019-02-28
DK3259345T3 (da) 2023-03-27
HUE041518T2 (hu) 2019-05-28
EP3259345B1 (en) 2023-02-08
HRP20230302T1 (hr) 2023-05-12
LT3059307T (lt) 2019-01-10
WO2016131959A1 (en) 2016-08-25
EP3059307B1 (en) 2018-10-17
ES2703167T3 (es) 2019-03-07
CN107532144A (zh) 2018-01-02
RS64062B1 (sr) 2023-04-28
DK3059307T3 (en) 2019-01-14
CA2986830A1 (en) 2016-08-25
PL3259345T3 (pl) 2023-05-02
HRP20182119T4 (hr) 2022-09-02
ES2703167T5 (es) 2022-09-30
ES2940191T3 (es) 2023-05-04
SI3259345T1 (sl) 2023-08-31
CY1121243T1 (el) 2020-05-29
PT3059307T (pt) 2019-01-23
EP3259345A1 (en) 2017-12-27
DK3059307T5 (da) 2022-08-29
JP7132463B2 (ja) 2022-09-07
FI3259345T3 (fi) 2023-04-03
PT3259345T (pt) 2023-03-15
RS58070B2 (sr) 2022-10-31
EP3059307A1 (en) 2016-08-24
DK3059307T4 (da) 2022-08-01
JP6840084B2 (ja) 2021-03-17
JP2018508207A (ja) 2018-03-29
US20240150721A1 (en) 2024-05-09
CN111548985A (zh) 2020-08-18

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