SG11201906225PA - Maintenance and expansion of pancreatic progenitor cells - Google Patents

Maintenance and expansion of pancreatic progenitor cells

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Publication number
SG11201906225PA
SG11201906225PA SG11201906225PA SG11201906225PA SG11201906225PA SG 11201906225P A SG11201906225P A SG 11201906225PA SG 11201906225P A SG11201906225P A SG 11201906225PA SG 11201906225P A SG11201906225P A SG 11201906225PA SG 11201906225P A SG11201906225P A SG 11201906225PA
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Singapore
Prior art keywords
international
singapore
pct
rule
pancreatic progenitor
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SG11201906225PA
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Jamie Trott
Norris Ray Dunn
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Agency Science Tech & Res
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Publication of SG11201906225PA publication Critical patent/SG11201906225PA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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    • C12N2513/00—3D culture
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

FigW O 20 18/ 136005 Al (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 26 July 2018 (26.07.2018) WIPO I PCT omit VIII °nolo oilimioonsomo oimIE (10) International Publication Number WO 2018/136005 Al (51) International Patent Classification: C12N 5/071 (2010.01) (21) International Application Number: PCT/SG2018/050029 (22) International Filing Date: 17 January 2018 (17.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201700390Q 17 January 2017 (17.01.2017) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis North Tower, Singapore 138632 (SG). (72) Inventors: TROTT, Jamie; c/o Institute of Medical Biol- ogy, 8A Biomedical Grove, #06-06 Immunos, Singapore 138648 (SG). DUNN, Norris Ray; c/o Institute of Medical Biology, 8A Biomedical Grove, #06-06 Immunos, Singa- pore 138648 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: of inventorship (Rule 4.17(iv)) (54) Title: MAINTENANCE AND EXPANSION OF PANCREATIC PROGENITOR CELLS (57) : The present invention relates to a method of culturing a pancreatic progenitor cell. The method comprises contacting the cell with epidermal growth factor (EGF), retinoic acid (RA) and an inhibitor of transforming growth factor-13 (TGF-13) and 3T3-J2 feeder cells. A cell produced by the method of the invention and a kit when used in the method are also provided. C. [Continued on next page] WO 2018/136005 Al MIDEDIMOHNOBEIEDEMIIMINNIMMENDIS Published: — with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) in black and white; the international application as filed contained color or greyscale and is available for download from PATENTSCOPE
SG11201906225PA 2017-01-17 2018-01-17 Maintenance and expansion of pancreatic progenitor cells SG11201906225PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10201700390Q 2017-01-17
PCT/SG2018/050029 WO2018136005A1 (en) 2017-01-17 2018-01-17 Maintenance and expansion of pancreatic progenitor cells

Publications (1)

Publication Number Publication Date
SG11201906225PA true SG11201906225PA (en) 2019-08-27

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SG11201906225PA SG11201906225PA (en) 2017-01-17 2018-01-17 Maintenance and expansion of pancreatic progenitor cells

Country Status (6)

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US (1) US20200140826A1 (en)
EP (1) EP3571291A4 (en)
JP (1) JP2020506679A (en)
CN (1) CN110392735A (en)
SG (1) SG11201906225PA (en)
WO (1) WO2018136005A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190085296A1 (en) * 2017-09-20 2019-03-21 Sergio Mora Method of expansion of human pancreas progenitor cells from stem cells using feeder-conditioned media
KR20210077698A (en) * 2018-10-15 2021-06-25 사이니티 가부시키가이샤 Method for producing stem/progenitor cells from endoderm tissue or organ-derived cells using low molecular weight compounds
JPWO2021221104A1 (en) * 2020-04-30 2021-11-04
CN111808795A (en) * 2020-06-28 2020-10-23 广东省医疗器械研究所 Hepatocyte in-vitro co-culture system and construction method and application thereof
CN113046299A (en) * 2021-03-19 2021-06-29 上海爱萨尔生物科技有限公司 Additive for preparing islet beta cells by inducing pluripotent stem cells to differentiate directionally
CN112961823B (en) * 2021-03-19 2024-01-23 上海爱萨尔生物科技有限公司 Culture solution for preparing islet beta cells by inducing directional differentiation of pluripotent stem cells

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2460602A1 (en) * 2004-03-05 2005-09-05 Raewyn Seaberg Pancreatic multipotent progenitor cells
WO2009143353A1 (en) * 2008-05-22 2009-11-26 Vesta Therapeutics, Inc. Method of differentiating mammalian progenitor cells into insulin producing pancreatic islet cells
CA2868392A1 (en) * 2012-05-23 2013-11-28 Estelle DOUDEMENT Compositions and methods of obtaining and using endoderm and hepatocyte cells
GB201216796D0 (en) * 2012-09-20 2012-11-07 Cambridge Entpr Ltd In vitro pancreatic differentiation
JP6494515B2 (en) * 2012-10-19 2019-04-03 エージェンシー フォー サイエンス， テクノロジー アンド リサーチ Method for differentiating stem cells into one or more cell lineages
US8859286B2 (en) * 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
CA2957000C (en) * 2014-08-04 2023-08-01 Takeda Pharmaceutical Company Limited Method for proliferation of pancreatic progenitor cells

Also Published As

Publication number Publication date
JP2020506679A (en) 2020-03-05
WO2018136005A1 (en) 2018-07-26
EP3571291A1 (en) 2019-11-27
US20200140826A1 (en) 2020-05-07
EP3571291A4 (en) 2020-08-19
CN110392735A (en) 2019-10-29

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