JP2009506770A - 前駆細胞株の誘導法 - Google Patents
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Abstract
【選択図】 図1
Description
共培養の不要
分化の偏倚
内胚葉前駆細胞
造血先駆細胞及び内皮前駆細胞
心臓中胚葉前駆細胞及び骨格筋芽細胞前駆細胞
胚様体の形成
富栄養培地
培養での長期維持
前駆細胞の特徴
自己複製及び分化の調節因子
前駆細胞及び幹細胞
分化細胞
i)含脂肪細胞:体内のどこにでもあり、特に皮膚下にある脂肪又は脂肪組織の機能細胞型。含脂肪細胞は、エネルギー、体温調節、及び機械衝撃に対する緩衝作用のために脂肪を蓄え、合成する。
ii)心筋細胞:心臓が連続的且つ周期的に鼓動することを可能にする心臓の機能筋細胞型。
iii)軟骨細胞:関節、外耳道、気管、喉頭蓋、喉頭、脊椎間、肋骨の端間の椎間板の軟骨を形成する機能細胞型。
iv)線維芽細胞:体の大部分の組織内に見つけられる結合又は支持細胞。線維芽細胞は、特定の臓器の機能細胞型が正しく機能することを支援する指導支持骨格を与える。
v)肝細胞:代謝廃棄物を解毒し、赤血球を破壊し、その構成要素を再生する酵素を作り、血漿タンパク質を合成する肝臓の機能細胞型。
vi)造血細胞:血液を作る機能細胞型。造血細胞は、成体の骨髄内にある。胎児では、造血細胞は、肝臓、脾臓、骨髄、及び子宮内の胎児を囲む支持組織内にある。
vii)筋細胞:筋肉の機能細胞型。
viii)神経細胞:インパルスの伝達に特化された脳の機能細胞型。
ix)骨芽細胞:骨形成に関与する機能細胞型。
x)島細胞:インスリン、グルカゴン、ガストリン、及びソマトスタチンの分泌に関与する膵臓の機能細胞。これらの分子は共に、炭水化物代謝及び脂肪代謝、血糖値、及び胃酸分泌を含む多数の作用を調節する。
前駆細胞及び分化細胞の使用法
薬剤スクリーニング
組織再生
癌
幹細胞
全能性幹細胞
多能性幹細胞
胚性幹細胞
胚性生殖細胞
成体幹細胞
複能性幹細胞
幹細胞源
培地及びフィーダー細胞
胚性幹細胞
胚生殖細胞
自己複製及び分化
自己複製
分化
実施例
実施例1.方法:E−RoSH細胞株の誘導
実施例2.方法:HuES9.E間葉系幹細胞(MSC)様細胞株の誘導
実施例3.方法:RT−PCR分析
実施例4.方法:インビトロでの内皮細胞の分化
実施例5.方法:インビボでの内皮細胞の分化
実施例6.マウス胚性幹細胞(mESC)からの系統限定された内皮前駆細胞株の誘導
実施例7.E−RoSH内皮前駆細胞の特徴付け
実施例8.インビトロ及びインビボでのE−RoSH細胞の内皮細胞への分化
実施例9.ヒト胚性幹(hES)細胞株からの系統限定された前駆細胞株の誘導
実施例9.ディスカッション
参考文献
2)Wobus AM,Boheler KR. Embryonic stem cells: prospects for developmental biology and cell therapy. Physiol Rev. 2005;85:635-678
3)Wiles MV. Embryonic stem cell differentiation in Vitro. Methods in Enzymology. 1993;225:900-918
4)Choi K,Chung YS、Zhang WJ. Hematopoietic and endothelial development of mouse embryonic stem cells in culture. Methods Mol Med. 2005;105:359-368
5)Yin Y,Que J,Teh M,Cao WP,El Oakley RM,Lim S-K. Embryonic Cell Lines with Endothelial Potential: An In Vitro System for Studying Endothelial Differentiation. Arterioscler Thromb Vasc Biol. 2004;24:691-696
6)Lim SK,Bieker JJ,Lin CS,Costantini F. A shortened life span of EKLF-/- adult erythrocytes,due to a deficiency of beta-globin chains,is ameliorated by human gamma-globin chains. Blood. 1997;90:1291-1299
7)Bourc'his D,Xu GL,Lin CS,Bollman B,Bestor TH. Dnmt3L and the establishment of maternal genomic imprints. Science. 2001;294:2536-2539
8)Wei CL,Miura T,Robson P,Lim SK,Xu XQ,Lee MY,Gupta S,Stanton L,Luo Y,Schmitt J,Thies S,Wang W,Khrebtukova I,Zhou D,Liu ET,Ruan YJ,Rao M,Lim B. Transcriptome profiling of human and murine ESCs identifies divergent paths required to maintain the stem cell state. Stem Cells. 2005;23:166-185
9)Rao M. Conserved and divergent paths that regulate self-renewal in mouse and human embryonic stem cells. Dev Biol. 2004;275:269-286
10)Jaffredo T,Bollerot K,Sugiyama D,Gautier R,Drevon C. Tracing the hemangioblast during embryogenesis: developmental relationships between endothelial and hematopoietic cells. Int J Dev Biol. 2005;49:269-277
11)De Robertis EM,Wessely O,Oelgeschlager M,Brizuela B,Pera E,Larrain J,Abreu J,Bachiller D. Molecular mechanisms of cell-cell signaling by the Spemann-Mangold organizer. Int J Dev Biol. 2001;45:189-197
12)Hart AH,Hartley L,Sourris K,Stadler ES,Li R、Stanley EG,Tam PP,Elefanty AG,Robb L. Mixl1 is required for axial mesendoderm morphogenesis and patterning in the murine embryo. Development. 2002;129:3597-3608
13)Mohn D,Chen SW,Dias DC,Weinstein DC,Dyer MA,Sahr K,Ducker CE,Zahradka E,Keller G,Zaret KS,Gudas LJ,Baron MH. Mouse Mix gene is activated early during differentiation of ES and F9 stem cells and induces endoderm in frog embryos. Dev Dyn. 2003;226:446-459
14)Seidah NG,Marcinkiewicz M,Benjannet S,Gaspar L,Beaubien G,Mattei MG,Lazure C,Mbikay M,Chretien M. Cloning and primary sequence of a mouse candidate prohormone convertase PC1 homologous to PC2, Furin, and Kex2: distinct chromosomal localization and messenger RNA distribution in brain and pituitary compared to PC2. Mol Endocrinol. 1991;5:111-122
15)Benjannet S,Rondeau N,Day R,Chretien M,Seidah NG. PC1 and PC2 are proprotein convertases capable of cleaving proopiomelanocortin at distinct pairs of basic residues. Proc Natl Acad Sci U S A. 1991;88:3564-3568
16)Cowan CA,Klimanskaya I,McMahon J,Atienza J,Witmyer J,Zucker JP,Wang S,Morton CC,McMahon AP,Powers D,Melton DA. Derivation of embryonic stem cell lines from human blastocysts. N Engl J Med. 2004;350:1353-1356
17)Que J,El Oakley RM,Salto-Tellez M,Wong N,de Kleijn DP,Teh M,Retnam L,Lim SK. Generation of hybrid cell lines with endothelial potential from spontaneous fusion of adult bone marrow cells with embryonic fibroblast feeder. In Vitro Cell Dev Biol Anim. 2004;40:143-149
18)Barry FP,Murphy JM. Mesenchymal stem cells: clinical applications and biological characterization. Int J Biochem Cell Biol. 2004;36:568-584
19)Barberi T,Willis LM,Socci ND,Studer L. Derivation of multipotent mesenchymal precursors from human embryonic stem cells. PLoS Med. 2005;2:e161
20)Robertson EJ. Embryo-derived stem cell lines. In: Robertson EJ, ed. Teratocarcinomas and embryonic stem cells: a practical approach. Oxford: IRL Press Limited; 1987:71-112
21)Pittenger MF,Mackay AM,Beck SC,Jaiswal RK,Douglas R,Mosca JD,Moorman MA,Simonetti DW,Craig S,Marshak et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143-147
Claims (39)
- (a)一の胚性幹(ES)細胞を供給することと、
(b)前記胚性幹細胞から一の前駆細胞株を樹立することと、
を含み、
前記前駆細胞株は、当該前駆細胞株の自己複製能に基づいて選択される、方法。 - 複数の体細胞は、当該複数の体細胞は自己複製ができないことに基づき、選択されない、請求項1に記載の方法。
- 前記前駆細胞株は、共培養なしで、好適には、複数のフィーダー細胞なしで誘導又は樹立される、請求項1又は2に記載の方法。
- 共培養がないことで、複数の胚性幹細胞は選択されない、請求項3に記載の方法。
- 前記前駆細胞株は、形質転換なしで樹立される、請求項1乃至4のうちいずれか一項に記載の方法。
- 前記前駆細胞株は、複数の胚性幹細胞又はその子孫細胞を、複数の推定前駆細胞の自己複製を促進する条件に暴露することにより樹立される、請求項1乃至5のうちいずれか一項に記載の方法。
- 自己複製を促進する前記条件は、複数の胚性幹細胞の増殖を抑制する、請求項6に記載の方法。
- 自己複製を促進する前記条件は、富栄養培地での成長を含む、請求項6又は7に記載の方法。
- 自己複製を促進する前記条件は、LIFなしで複数の細胞を成長させることを含む、請求項6乃至8のうちいずれか一項に記載の方法。
- 自己複製を促進する前記条件は、連続継代を含む、請求項6乃至9のうちいずれか一項に記載の方法。
- 自己複製を促進する前記条件は、少なくとも12の連続継代を含む、請求項6乃至10のうちいずれか一項に記載の方法。
- 前記前駆細胞株は、前記胚性幹細胞に比べて能力が減少する、請求項1乃至11のうちいずれか一項に記載の方法。
- 前記前駆細胞株は、系統限定される、好適には、非多能性である、請求項1乃至12のうちいずれか一項に記載の方法。
- 前記前駆細胞株は、非発癌性である、請求項1乃至13のうちいずれか一項に記載の方法。
- 前記前駆細胞株を誘導することは、前記胚性幹細胞を、一の特定系統への分化を増進する条件に暴露することを含む、請求項1乃至14のうちいずれか一項に記載の方法。
- 分化を増進する前記条件は、前記胚性幹細胞から一の胚様体を形成することを含む、請求項15に記載の方法。
- 多分化能がなくなった後、前記複数の細胞は、分化を促進する前記条件から外される、請求項15又は16に記載の方法。
- 前記系統限定−促進条件から前記複数の細胞を外すことは、一の胚様体を分離することを含む、請求項15乃至17のうちいずれか一項に記載の方法。
- 約3−6日間成長させられた複数の胚様体を分離することを含む、請求項15乃至18のいずれか一項に記載の方法。
- 前記前駆細胞株は、OCT4及びアルカリホスファターゼ活性のいずれか又は両方の発現を減少するか、又は、それらの発現は実質的にない、請求項1乃至19のうちいずれか一項に記載の方法。
- 前記前駆細胞株は、当該前駆細胞株が誘導される一の胚性幹細胞に比べて、一の多分化能マーカーの発現を減少し、
前記多分化能マーカーは、好適には、Nanog、BMP4、FGF5、Oct4、Sox−2、及びUtf1から構成される群から選択される、請求項1乃至20のうちいずれか一項に記載の方法。 - 前記前駆細胞株は、
(a)40世代を超えて細胞培養中で維持可能である特徴と、
(b)少なくとも10世代の間、細胞培養中で維持される場合に実質的に安定した核型又は染色体数を有する特徴と、
(c)何世代にも亘って実質的に安定した遺伝子発現パターンを有する特徴と、
のうち1つ以上の特徴を示す、請求項1乃至21のうちいずれか一項に記載の方法。 - 前記前駆細胞株は、好適には免疫力が低下した被移植動物である被移植動物に移植される場合、好適には3週間後、より好適には2乃至9ヶ月後でも奇形腫の形成を実質的に誘発しない、請求項1乃至22のうちいずれか一項に記載の方法。
- 前記胚性幹細胞又は前記前駆細胞株は、哺乳類細胞又は哺乳類細胞株であり、好適にはマウス又はヒトの胚性幹細胞又は前駆細胞株である、請求項1乃至23のうちいずれか一項に記載の方法。
- 前記前駆細胞株は、内皮前駆細胞株、好適にはE−RoSH細胞株を含む、請求項1乃至24のうちいずれか一項に記載の方法。
- 前記前駆細胞株は、間葉系前駆細胞株、好適にはhuES9.E1細胞株を含む、請求項1乃至24のうちいずれか一項に記載の方法。
- (d)前記前駆細胞株から一の分化細胞を誘導することをさらに含む、請求項1乃至26のうちいずれか一項に記載の方法。
- 前記前駆細胞株は、分化の前に少なくとも5世代の間増殖される、請求項27に記載の方法。
- 一の胚性幹(ES)細胞から一の分化細胞を形成する、請求項27又は28に記載の方法。
- 前記分化細胞は、内皮細胞又は間葉細胞である、請求項27乃至29のうちいずれか一項に記載の方法。
- 前記分化細胞は、含脂肪細胞又は骨細胞である、請求項27乃至30に記載の方法。
- 一の細胞の間葉マーカー又は内皮マーカーの発現を上方調節する、請求項1乃至31のうちいずれか一項に記載の方法。
- 一の細胞の幹細胞マーカー又は多分化能マーカーの発現を下方調節する、請求項1乃至32のうちいずれか一項に記載の方法。
- 一の幹細胞の自己複製又は分化を促進又は遅延可能な一の作用因子を特定する方法であって、
一の候補分子の存在下で請求項1乃至33のうちいずれか一項に記載する方法を実行することと、
前記候補分子への影響を決定することと、
を含む方法。 - 再生療法により治療可能な疾病、心臓疾患、骨髄疾患、皮膚疾患、火傷、また、糖尿病、アルツハイマー病、パーキンソン病といった変性疾患、及び癌のうちのいずれか1つの治療、又は、治療のための一の薬剤組成物の調製の目的で、一の前駆細胞株又は一の分化細胞を形成する請求項1乃至34のうちいずれか一項に記載の方法。
- 請求項1乃至26のうちいずれか一項に記載する方法により形成される前駆細胞株。
- 請求項27乃至31のうちいずれか一項に記載する方法により形成される分化細胞。
- 一の胚性幹(ES)細胞から一の分化細胞を形成する方法であって、
(a)前記胚性幹細胞から一の前駆細胞株を誘導することと、
(b)前記前駆細胞株を増殖することと、
(c)前記前駆細胞株から一の分化細胞を誘導することと、
を含む方法。 - (a)一の胚性幹(ES)細胞を供給することと、
(b)前記胚性幹細胞から一の前駆細胞を誘導することと、
(c)前記前駆細胞から一の前駆細胞株を樹立することと、
を含み、
前記前駆細胞は、当該前駆細胞の自己複製能に基づいて選択される、方法。
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014519854A (ja) * | 2011-06-23 | 2014-08-21 | ザ チルドレンズ ホスピタル オブ フィラデルフィア | ヒト多能性幹細胞から生じる自己複製する内胚葉前駆細胞株とその使用方法 |
Families Citing this family (78)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8017395B2 (en) | 2004-12-17 | 2011-09-13 | Lifescan, Inc. | Seeding cells on porous supports |
PT1888123E (pt) | 2005-06-08 | 2013-03-13 | Janssen Biotech Inc | Uma terapêutica celular para a degeneração ocular |
KR20140146224A (ko) | 2006-04-14 | 2014-12-24 | 어드밴스드 셀 테크놀로지, 인코포레이티드 | 혈관 콜로니 형성 세포 |
US8741643B2 (en) | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
US8628964B2 (en) * | 2006-10-11 | 2014-01-14 | Drexel University | Fetal pulmonary cells and uses thereof |
US8440461B2 (en) | 2007-03-23 | 2013-05-14 | Wisconsin Alumni Research Foundation | Reprogramming somatic cells using retroviral vectors comprising Oct-4 and Sox2 genes |
WO2008150498A1 (en) * | 2007-05-30 | 2008-12-11 | University Of Georgia Research Foundation Inc. | Human embryonic stem cell derived mesoderm-like epithelium transitions to mesenchymal progenitor cells |
WO2008151390A1 (en) * | 2007-06-15 | 2008-12-18 | Australian Stem Cell Centre Ltd | Differentiation of human embryonic stem cells |
US9080145B2 (en) | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
WO2009018453A1 (en) | 2007-07-31 | 2009-02-05 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
US9700582B2 (en) | 2007-09-11 | 2017-07-11 | Sapporo Medical University | Cell growth method and pharmaceutical preparation for tissue repair and regeneration |
US20110236971A2 (en) | 2007-09-25 | 2011-09-29 | Maksym Vodyanyk | Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions |
CA2706560C (en) | 2007-11-27 | 2017-02-28 | Lifescan, Inc. | Differentiation of human embryonic stem cells to pancreatic cells |
US8512696B2 (en) * | 2007-11-30 | 2013-08-20 | Autologous, Llc | Methods of isolating non-senescent cardiac stem cells and uses thereof |
JP5733986B2 (ja) | 2008-02-21 | 2015-06-10 | ヤンセン バイオテツク,インコーポレーテツド | 細胞の付着、培養、及び剥離のための方法、表面改質されたプレート、並びに組成物 |
KR101077042B1 (ko) * | 2008-03-28 | 2011-10-26 | 경희대학교 산학협력단 | 중배엽 줄기세포의 증식을 촉진하기 위한 cd45+ 세포 또는 cd45+ 세포 배양액의 용도 |
US8470595B2 (en) * | 2008-04-18 | 2013-06-25 | National University Corporation Nagoya University | Mesenchymal stem cell and method for production thereof |
US7939322B2 (en) | 2008-04-24 | 2011-05-10 | Centocor Ortho Biotech Inc. | Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm |
US8623648B2 (en) | 2008-04-24 | 2014-01-07 | Janssen Biotech, Inc. | Treatment of pluripotent cells |
CN104962514B (zh) | 2008-05-27 | 2021-04-02 | 奥列弗·D·博斯 | 人骨骼肌中的褐色脂肪细胞祖细胞 |
PL2942392T3 (pl) | 2008-06-30 | 2019-02-28 | Janssen Biotech, Inc | Różnicowanie pluripotencjalnych komórek macierzystych |
WO2010040262A1 (zh) * | 2008-10-10 | 2010-04-15 | 深圳市嘉天源生物科技有限公司 | 分离动物胚胎间质性干细胞并提取其分泌物的方法 |
MX349178B (es) | 2008-10-31 | 2017-07-17 | Centocor Ortho Biotech Inc | Diferenciación de células madre embrionarias humanas al linaje endocrino pancreático. |
WO2010051223A1 (en) | 2008-10-31 | 2010-05-06 | Centocor Ortho Biotech Inc. | Differentiation of human embryonic stem cells to the pancreatic endocrine lineage |
KR101687344B1 (ko) | 2008-11-20 | 2016-12-16 | 얀센 바이오테크 인코포레이티드 | 평면 기재상의 세포 부착 및 배양을 위한 방법 및 조성물 |
EP2366021B1 (en) | 2008-11-20 | 2017-08-02 | Janssen Biotech, Inc. | Pluripotent stem cell culture on micro-carriers |
US8790638B2 (en) * | 2009-02-04 | 2014-07-29 | Stemedica Cell Technologies, Inc. | Compositions of stem cells and stem cell factors and methods for their use and manufacture |
KR101032271B1 (ko) * | 2009-05-25 | 2011-05-06 | (주)미래생명공학연구소 | 피부세포 재생용 조성물, 그 제조 방법, 피부세포 재생 방법 및 화장료 조성물 |
JPWO2010137722A1 (ja) * | 2009-05-26 | 2012-11-15 | 武田薬品工業株式会社 | 再生医薬のスクリーニング方法 |
EP2456858B1 (en) | 2009-07-20 | 2018-08-29 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
WO2011011300A2 (en) | 2009-07-20 | 2011-01-27 | Centocor Ortho Biotech Inc. | Differentiation of human embryonic stem cells |
SG177416A1 (en) | 2009-07-20 | 2012-02-28 | Janssen Biotech Inc | Differentiation of human embryonic stem cells |
SG10201403202XA (en) * | 2009-07-23 | 2014-08-28 | Agency Science Tech & Res | Pre-natal mesenchymal stem cells |
CN102858997B (zh) | 2009-11-02 | 2014-06-18 | 新加坡科技研究局 | 用于监测细胞状态和用于永生间充质干细胞的方法 |
RU2610176C2 (ru) | 2009-12-23 | 2017-02-08 | Янссен Байотек, Инк. | Дифференцировка человеческих эмбриональных стволовых клеток |
MX339669B (es) | 2009-12-23 | 2016-06-02 | Janssen Biotech Inc | Diferenciacion de celulas madres embrionarias humanas. |
US20110206647A1 (en) * | 2010-02-25 | 2011-08-25 | Abt Holding Company | Modulation of Angiogenesis |
MX358451B (es) | 2010-03-01 | 2018-08-20 | Janssen Biotech Inc | Métodos para purificar células derivadas de células madre pluripotenciales. |
ES2728900T3 (es) | 2010-05-12 | 2019-10-29 | Janssen Biotech Inc | Diferenciación de células madre embrionarias humanas |
US20130330300A1 (en) | 2010-07-20 | 2013-12-12 | University Of Southern California | High Telomerase Activity Bone Marrow Mesenchymal Stem Cells, Methods of Producing the Same and Pharmaceuticals and Treatment Methods Based Thereon |
JP6218605B2 (ja) | 2010-08-31 | 2017-10-25 | ヤンセン バイオテツク,インコーポレーテツド | ヒト胚性幹細胞の分化 |
WO2012030538A2 (en) | 2010-08-31 | 2012-03-08 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
CA2809305C (en) | 2010-08-31 | 2019-06-11 | Janssen Biotech, Inc. | Differentiation of pluripotent stem cells |
WO2012048093A2 (en) | 2010-10-08 | 2012-04-12 | Osiris Therapeutics, Inc. | Enhanced msc preparations |
US20130309209A1 (en) * | 2010-10-22 | 2013-11-21 | Center For Regenerative Medicine Of Barcelona | Formation of hematopoietic progenitor cells from mesenchymal stem cells |
CN103648509B (zh) | 2011-03-11 | 2019-02-22 | 儿童医学中心公司 | 与间充质干细胞外来体相关的方法和组合物 |
EP2733204A4 (en) | 2011-07-11 | 2014-10-01 | Univ Kumamoto Nat Univ Corp | METHOD FOR THE PRODUCTION OF A PLURIPOTENTIAL CELL WITH FERMENTABLE BACTERIUM |
EP2776557A4 (en) | 2011-11-10 | 2015-04-08 | Energesis Pharmaceuticals Inc | BROWN ADIPOCYTE FOLLOWERS IN HUMAN SKELETAL MUSCLES |
JP6344821B2 (ja) * | 2011-11-21 | 2018-06-20 | ユニバーシティ・ヘルス・ネットワーク | 造血前駆体の集団および造血前駆体のための幹細胞を富化する方法 |
SG11201402649QA (en) | 2011-11-30 | 2014-06-27 | Agency Science Tech & Res | Gm1 ganglioside to annexin v microparticle polypeptide ratio for biological monitoring |
AU2012355698B2 (en) | 2011-12-22 | 2018-11-29 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
AU2013230020B2 (en) | 2012-03-07 | 2018-08-09 | Janssen Biotech, Inc. | Defined media for expansion and maintenance of pluripotent stem cells |
KR101422559B1 (ko) * | 2012-03-09 | 2014-07-24 | 창원대학교 산학협력단 | 지방유래 줄기세포 배양액, 이의 제조방법, 및 이를 포함하는 발모촉진용 조성물 |
EP3450542B1 (en) | 2012-06-08 | 2021-09-01 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into pancreatic endocrine cells |
CN104487568B (zh) * | 2012-07-11 | 2017-08-15 | 爱姆斯坦生物技术公司 | 人胚胎干细胞衍生的间充质样干细胞、方法及其应用 |
ES2837763T3 (es) | 2012-12-31 | 2021-07-01 | Janssen Biotech Inc | Cultivo de células madre embrionarias humanas en la interconexión aire-líquido para la diferenciación en células endocrinas pancreáticas |
SG11201505112SA (en) | 2012-12-31 | 2015-07-30 | Janssen Biotech Inc | Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators |
KR20150103203A (ko) | 2012-12-31 | 2015-09-09 | 얀센 바이오테크 인코포레이티드 | 췌장 내분비 세포 내로의 분화를 위한 인간 만능 세포의 현탁 및 클러스터링 |
US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
CN103509753B (zh) * | 2013-08-12 | 2016-06-15 | 中国科学院广州生物医药与健康研究院 | 一种人体造血干细胞分化培养方法 |
TWI486447B (zh) * | 2013-09-05 | 2015-06-01 | Buddhist Tzu Chi Medical Foundation | 人類間質幹細胞體外快速增生之佐劑、體外快速擴增人類間質幹細胞之方法、體外快速擴增人類間質幹細胞以獲取生長因子的方法及其用途 |
DK3143127T3 (da) | 2014-05-16 | 2021-09-13 | Janssen Biotech Inc | Anvendelse af små molekyler til at forstærke mafa-ekspression i endokrine pankreasceller |
AU2015264519B2 (en) | 2014-05-18 | 2021-01-28 | Children's Medical Center Corporation | Methods and compositions relating to exosomes |
CN113957043A (zh) * | 2015-03-04 | 2022-01-21 | 迈索布拉斯特国际有限公司 | 间充质干细胞的细胞培养方法 |
CN105624104B (zh) * | 2015-06-01 | 2018-10-12 | 中国医学科学院血液病医院(血液学研究所) | 一种提高人间充质干细胞功能的细胞处理方法 |
AU2017235446A1 (en) * | 2016-03-16 | 2018-11-08 | Cell Medicine, Inc. | Mesenchymal stem cells with enhanced efficacy |
MA45479A (fr) | 2016-04-14 | 2019-02-20 | Janssen Biotech Inc | Différenciation de cellules souches pluripotentes en cellules de l'endoderme de l'intestin moyen |
CN106085953A (zh) * | 2016-07-19 | 2016-11-09 | 安徽惠恩生物科技股份有限公司 | 一种临床级脐带间质细胞提取制备方法 |
US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
CN111093681A (zh) * | 2017-07-16 | 2020-05-01 | 雷蒙特亚特特拉维夫大学有限公司 | 人类口腔粘膜干细胞分泌蛋白质组 |
WO2019093481A1 (ja) * | 2017-11-09 | 2019-05-16 | 北海道公立大学法人札幌医科大学 | 組織再生用医薬及びその製造方法 |
US11285177B2 (en) | 2018-01-03 | 2022-03-29 | Globus Medical, Inc. | Allografts containing viable cells and methods thereof |
CN108570447A (zh) * | 2018-01-26 | 2018-09-25 | 皓昇莱生物制药有限公司 | 一种筛选分化hPSCs向MSCs的方法 |
CN109943525B (zh) * | 2019-03-15 | 2021-07-30 | 中科睿极(深圳)医学科技有限公司 | 无血清、无动物源成分、组分明确的培养基及其应用 |
JP6712740B1 (ja) * | 2019-03-25 | 2020-06-24 | 学校法人東海大学 | Tie2陽性幹/前駆細胞を含む細胞集団の培養方法およびその利用 |
KR102280509B1 (ko) * | 2020-09-04 | 2021-07-22 | 건국대학교 글로컬산학협력단 | 전분화능 줄기세포 기반 자가면역성 및 염증성 피부질환 예방 또는 치료용 조성물 및 이의 제조방법 |
CN112430626A (zh) * | 2020-11-27 | 2021-03-02 | 成都康景生物科技有限公司 | 一种基因修饰的脐间充质干细胞、制备方法及应用 |
CN116836920B (zh) * | 2023-08-21 | 2024-05-24 | 广东横琴粤澳深度合作区齐美国际干细胞医院有限公司 | 一种无血清培养基及其制备间充质干细胞的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004275145A (ja) * | 2003-03-18 | 2004-10-07 | Keio Gijuku | 単球由来多能性細胞momc |
WO2005024004A1 (ja) * | 2003-08-27 | 2005-03-17 | Renomedix Institute Inc. | 間葉系幹細胞の肝細胞への分化方法及び人工ヒト肝臓細胞 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69922933T2 (de) * | 1998-03-13 | 2005-12-29 | Osiris Therapeutics, Inc. | Anwendungen für humane nicht autologe, mesenchymale stammzellen |
WO2000053795A1 (en) | 1999-03-10 | 2000-09-14 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Adipose-derived stem cells and lattices |
US20030082152A1 (en) * | 1999-03-10 | 2003-05-01 | Hedrick Marc H. | Adipose-derived stem cells and lattices |
US7005252B1 (en) * | 2000-03-09 | 2006-02-28 | Wisconsin Alumni Research Foundation | Serum free cultivation of primate embryonic stem cells |
US7439064B2 (en) * | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
US7011828B2 (en) * | 2000-03-14 | 2006-03-14 | Es Cell International Pte. Ltd. | Implanting neural progenitor cells derived for human embryonic stem cells |
KR100903755B1 (ko) * | 2000-05-17 | 2009-06-18 | 제론 코포레이션 | 신경 선조세포 집단 |
CA2424062A1 (en) * | 2000-09-29 | 2002-04-04 | Derek Van Der Kooy | Primitive neural stem cells and method for differentiation of stem cells to neural cells |
US20030021771A1 (en) | 2001-07-06 | 2003-01-30 | Chunhui Xu | Osteoblast precursors from human embryonic stem cells |
WO2003086373A1 (en) * | 2002-04-12 | 2003-10-23 | Celgene Corporation | Methods for identification of modulators of angiogenesis, compounds discovered thereby, and methods of treatment using the compounds |
JPWO2003087349A1 (ja) * | 2002-04-17 | 2005-08-18 | 大塚製薬株式会社 | 間葉系細胞から膵β細胞を形成する方法 |
US20040052771A1 (en) * | 2002-07-12 | 2004-03-18 | Lim Sai Kiang | Hemangioblast progenitor cells |
JP2005304443A (ja) * | 2004-04-26 | 2005-11-04 | Institute Of Physical & Chemical Research | 前駆間葉系幹細胞 |
EP1743023A1 (en) * | 2004-05-07 | 2007-01-17 | Wisconsin Alumni Research Foundation | Method of forming mesenchymal stem cells from embryonic stem cells |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004275145A (ja) * | 2003-03-18 | 2004-10-07 | Keio Gijuku | 単球由来多能性細胞momc |
WO2005024004A1 (ja) * | 2003-08-27 | 2005-03-17 | Renomedix Institute Inc. | 間葉系幹細胞の肝細胞への分化方法及び人工ヒト肝臓細胞 |
Non-Patent Citations (2)
Title |
---|
JPN6011061105; The Proceedings of the National Academy of Sciences USA Vol. 97, No. 21, 200010, pp. 11307-11312 * |
JPN6012008146; 細胞工学 vol. 24, 200507, 712-716 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014519854A (ja) * | 2011-06-23 | 2014-08-21 | ザ チルドレンズ ホスピタル オブ フィラデルフィア | ヒト多能性幹細胞から生じる自己複製する内胚葉前駆細胞株とその使用方法 |
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WO2007027157A1 (en) | 2007-03-08 |
CN101341245A (zh) | 2009-01-07 |
US9018005B2 (en) | 2015-04-28 |
JP2009506769A (ja) | 2009-02-19 |
US8962318B2 (en) | 2015-02-24 |
BRPI0617085A2 (pt) | 2016-11-08 |
US20110014692A1 (en) | 2011-01-20 |
US9005897B2 (en) | 2015-04-14 |
AU2006285467A1 (en) | 2007-03-08 |
US20080199849A1 (en) | 2008-08-21 |
US20130280719A1 (en) | 2013-10-24 |
WO2007027156A1 (en) | 2007-03-08 |
IL189878A0 (en) | 2008-11-03 |
IL189882A0 (en) | 2008-11-03 |
AU2006285468A1 (en) | 2007-03-08 |
EP1937801A1 (en) | 2008-07-02 |
CN101341244A (zh) | 2009-01-07 |
KR20080056181A (ko) | 2008-06-20 |
KR20080056182A (ko) | 2008-06-20 |
US20080219957A1 (en) | 2008-09-11 |
BRPI0617084A2 (pt) | 2011-07-12 |
US20150197725A1 (en) | 2015-07-16 |
US20110008298A1 (en) | 2011-01-13 |
EP1943334A1 (en) | 2008-07-16 |
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