YU92592A - Biopostupci za dobijanje 7-ack i 7-adkc - Google Patents

Biopostupci za dobijanje 7-ack i 7-adkc

Info

Publication number
YU92592A
YU92592A YU92592A YU92592A YU92592A YU 92592 A YU92592 A YU 92592A YU 92592 A YU92592 A YU 92592A YU 92592 A YU92592 A YU 92592A YU 92592 A YU92592 A YU 92592A
Authority
YU
Yugoslavia
Prior art keywords
adipoyl
side chain
aca
acid
whose expression
Prior art date
Application number
YU92592A
Other languages
English (en)
Other versions
YU48930B (sh
Inventor
M.J. Conder
A.M. Stepan
L. Crawford
J.A. Rambosek
P.C. Mcada
C.D. Reeves
Original Assignee
Gist Brocades B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades B.V. filed Critical Gist Brocades B.V.
Publication of YU92592A publication Critical patent/YU92592A/sh
Publication of YU48930B publication Critical patent/YU48930B/sh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C12P37/04—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by acylation of the substituent in the 6 position
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/933—Penicillium
    • Y10S435/935—Penicillium chrysogenum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Medicinal Preparation (AREA)

Abstract

BIOPOSTUPCI ZA DOBIJANJE 7-ACK I 7-ADKC, važni intermedijeri za dobijanje cefalosporinskih antibiotika, 7-amino-cefalosporanska kiselina (7-ACK) i 7-aminodeacetilcefalosporanska kiselina (7-ADKC), dobijaju se novim biopostupkom u kome se transformisani Penicillium chrysogenum soj gaji u prisustvu adipatne sirovine za napajanje da bi se dobila adipoil-6-APK (6-amino penicilanska kiselina), a zatim in situ ekspresijom sledećih gena sa kojima je P. chrysogenum bio transformisan: 1) ekspandaza gena na pr., iz Cephalosporium acremonium, čiji ekspresioni proizvod konvertuje adipoil-6-APK ekspanzijom prstena u adipoil-7-ADCK; 2) hidroksilaza gena čiji ekspresioni proizvod konvertuje 3-metil bočni lanac adipoil-7-ADCK u 3-hidroksimetil, pri čemu daje prvi proizvod, 7-amino-deacetilcefalosporansku kiselinu (7-ADKC); i 3) acetiltransferaza gena čiji ekspresioni proizvod konvertuje 3-hidroksimetil bočni lanac u 3-acetiloksimetil bočni lanac 7-ACK. Finalni proizvod, 7-ACK, se tada dobija cepanjem adipoil bočnog lanca koristeći adipoil acilazu. Prema tome celokupna sinteza se izvodi koristeći biopostupke, i efikasna je i ekonomična.
YU92592A 1991-10-15 1992-10-15 Novi biopostupci za dobijanje 7-ack i 7-adkc YU48930B (sh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77783391A 1991-10-15 1991-10-15
US95349292A 1992-10-06 1992-10-06

Publications (2)

Publication Number Publication Date
YU92592A true YU92592A (sh) 1995-10-03
YU48930B YU48930B (sh) 2002-12-10

Family

ID=27119363

Family Applications (1)

Application Number Title Priority Date Filing Date
YU92592A YU48930B (sh) 1991-10-15 1992-10-15 Novi biopostupci za dobijanje 7-ack i 7-adkc

Country Status (27)

Country Link
US (4) US5559005A (sh)
EP (2) EP0540210B1 (sh)
JP (1) JP2655790B2 (sh)
KR (1) KR100227711B1 (sh)
CN (1) CN1074484A (sh)
AT (2) ATE174057T1 (sh)
AU (1) AU657800B2 (sh)
BG (1) BG62261B1 (sh)
CA (1) CA2080573C (sh)
CZ (1) CZ288721B6 (sh)
DE (2) DE69227750T2 (sh)
DK (2) DK0856516T3 (sh)
ES (2) ES2176613T3 (sh)
FI (1) FI106965B (sh)
GR (1) GR3029343T3 (sh)
HU (1) HU219370B (sh)
IL (3) IL103419A (sh)
MX (1) MX9205902A (sh)
NO (1) NO315802B1 (sh)
NZ (1) NZ244714A (sh)
RO (1) RO116648B1 (sh)
RU (1) RU2002130702A (sh)
SK (1) SK280569B6 (sh)
TW (1) TW272233B (sh)
UA (1) UA47385C2 (sh)
WO (1) WO1993008287A1 (sh)
YU (1) YU48930B (sh)

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KR0171897B1 (ko) * 1989-12-27 1999-02-01 후지사와 도모끼찌로 7-아미노세펨 화합물 또는 그 염류의 제조 방법
US5318896A (en) * 1991-09-11 1994-06-07 Merck & Co., Inc. Recombinant expandase bioprocess for preparing 7-aminodesacetoxy cephalosporanic acid (7-ADCA)
SK282617B6 (sk) * 1993-07-30 2002-10-08 Dsm N. V. Spôsob výroby a izolácie 7-aminodesacetoxycefalosporánovej kyseliny
DE69434023D1 (de) * 1993-07-30 2004-10-28 Dsm Ip Assets Bv Verfahren zur effizienten Herstellung von 7-ADCA via 2-(carboxyethylthio)acetyl-7-ADCA und 3-(carboxymethylthio)propionyl-7-ADCA
JP3764166B2 (ja) * 1994-09-28 2006-04-05 ノボザイムス アクティーゼルスカブ 二次代謝産物を産生するための方法
DE4437420A1 (de) * 1994-10-19 1996-04-25 Hoechst Ag Verbessertes, induktionsfreies Verfahren zur gentechnischen Herstellung von Glutarylamidase
US5731165A (en) * 1995-06-02 1998-03-24 Gist-Brocades, B.V. Process for the production of 7-ADCA via expandase activity on penicillin G
PL182509B1 (pl) * 1995-06-02 2002-01-31 Gist Brocades Bv Sposób wytwarzania kwasu 7-aminodezacetoksycefalosporanowego (7-ADCA)
AU2508897A (en) * 1996-04-04 1997-10-29 Gist-Brocades B.V. An enzyme with high adipoyl-coenzyme a synthetase activity and uses thereof
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WO1999010499A1 (en) * 1997-08-22 1999-03-04 Dsm N.V. Statin production by fermentation
ES2186347T3 (es) 1998-03-27 2003-05-01 Dsm Nv Nuevo procedimiento de produccion por fermentacion de cefalosporina.
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ES2165276B1 (es) * 1999-06-18 2003-03-01 Antibioticos Sau Procedimiento biotecnologico para la produccion de acido 7-aminocefal osporanico (7-adca) y compuestos de sintesis intermedios particularmente penicilina n y desacetoxicefalosporina c (daoc)
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JP2006528970A (ja) * 2003-05-28 2006-12-28 デーエスエム アイピー アセッツ ベー． ヴェー． セフェム化合物
ES2423454T3 (es) * 2006-02-23 2013-09-20 Dsm Sinochem Pharmaceuticals Netherlands B.V. Producción mejorada de cefalosporina
KR101569832B1 (ko) * 2008-11-19 2015-11-18 삼성전자주식회사 시료 중의 게놈 ｄｎａ 및 플라스미드 ｄｎａ를 서로 분리하는 방법 및 그를 위한 키트
CN101429538B (zh) * 2008-12-12 2011-05-25 河北九派制药有限公司 7-α-甲氧基-3-去乙酰基头孢噻吩苄星盐的制备方法
CN102369290A (zh) 2009-04-03 2012-03-07 帝斯曼知识产权资产管理有限公司 发酵工艺
US8863444B2 (en) 2010-05-31 2014-10-21 Feature Walters Assembly system for modular building units
CN103003439A (zh) 2010-06-22 2013-03-27 中化帝斯曼制药有限公司荷兰公司 气泡发酵工艺
CN103108879B (zh) 2010-09-07 2016-08-17 中化帝斯曼制药有限公司荷兰公司 用于生产头孢菌素类的方法
CN102702229A (zh) * 2011-08-04 2012-10-03 重庆天地药业有限责任公司 一种头孢唑肟钠关键中间体的制备及高含量头孢唑肟钠的制备
WO2015091455A1 (en) 2013-12-17 2015-06-25 Dpx Holdings B.V. Bioreactor
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Also Published As

Publication number Publication date
US5559005A (en) 1996-09-24
TW272233B (sh) 1996-03-11
NO941345D0 (no) 1994-04-14
ATE174057T1 (de) 1998-12-15
ES2176613T3 (es) 2002-12-01
RO116648B1 (ro) 2001-04-30
BG98714A (bg) 1995-02-28
AU657800B2 (en) 1995-03-23
GR3029343T3 (en) 1999-05-28
DK0540210T3 (da) 1999-06-23
DE69232581T2 (de) 2002-11-14
JP2655790B2 (ja) 1997-09-24
FI106965B (fi) 2001-05-15
FI941730A (fi) 1994-04-14
DE69227750T2 (de) 1999-04-22
BG62261B1 (bg) 1999-06-30
WO1993008287A1 (en) 1993-04-29
IL133733A0 (en) 2001-04-30
UA47385C2 (uk) 2002-07-15
IL133734A0 (en) 2001-04-30
CZ288721B6 (cs) 2001-08-15
EP0540210A1 (en) 1993-05-05
HUT69783A (en) 1995-09-28
HU219370B (en) 2001-03-28
NZ244714A (en) 1994-03-25
CN1074484A (zh) 1993-07-21
CA2080573C (en) 2004-01-06
US5629171A (en) 1997-05-13
AU2701692A (en) 1993-04-22
ATE216700T1 (de) 2002-05-15
DE69227750D1 (de) 1999-01-14
EP0856516A1 (en) 1998-08-05
CZ88494A3 (en) 1995-03-15
EP0856516B1 (en) 2002-04-24
IL103419A0 (en) 1993-03-15
DK0856516T3 (da) 2002-07-29
MX9205902A (es) 1994-06-30
US6017726A (en) 2000-01-25
US6071713A (en) 2000-06-06
YU48930B (sh) 2002-12-10
HU9401073D0 (en) 1994-07-28
SK43194A3 (en) 1996-11-06
ES2127205T3 (es) 1999-04-16
CA2080573A1 (en) 1993-04-16
SK280569B6 (sk) 2000-03-13
EP0540210B1 (en) 1998-12-02
DE69232581D1 (de) 2002-05-29
RU2002130702A (ru) 2004-05-20
IL103419A (en) 2003-06-24
NO941345L (no) 1994-06-15
NO315802B1 (no) 2003-10-27
KR100227711B1 (ko) 1999-12-01
JPH06113884A (ja) 1994-04-26
FI941730A0 (fi) 1994-04-14

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