WO2000037671A3 - Improved in vivo production of cephalosporins - Google Patents

Improved in vivo production of cephalosporins Download PDF

Info

Publication number
WO2000037671A3
WO2000037671A3 PCT/EP1999/010292 EP9910292W WO0037671A3 WO 2000037671 A3 WO2000037671 A3 WO 2000037671A3 EP 9910292 W EP9910292 W EP 9910292W WO 0037671 A3 WO0037671 A3 WO 0037671A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleotide sequence
aca
acylated
side chain
produced
Prior art date
Application number
PCT/EP1999/010292
Other languages
French (fr)
Other versions
WO2000037671A2 (en
Inventor
Roelof Ary Lans Bovenberg
Richard Kerkman
Eric Koenhen
Original Assignee
Dsm Nv
Roelof Ary Lans Bovenberg
Richard Kerkman
Eric Koenhen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Nv, Roelof Ary Lans Bovenberg, Richard Kerkman, Eric Koenhen filed Critical Dsm Nv
Priority to KR1020017007915A priority Critical patent/KR20010089672A/en
Priority to EP99964657A priority patent/EP1141372A2/en
Priority to JP2000589724A priority patent/JP2002533092A/en
Priority to AU30426/00A priority patent/AU3042600A/en
Publication of WO2000037671A2 publication Critical patent/WO2000037671A2/en
Publication of WO2000037671A3 publication Critical patent/WO2000037671A3/en
Priority to HK02103286.5A priority patent/HK1041610A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention discloses a process for the production of 7-amino cephalosporanic acid (7-ACA) or a derivative thereof comprising the steps of fermenting a P. chrysogenum strain being transformed with an expression construct comprising a nucleotide sequence encoding expandase, hydroxylase and acetyltransferase activity in the presence of a suitable acyl side chain precursor, or a salt or ester thereof, such that an N-acylated 7-ACA compound is produced, N-deacylating the thus produced N-acylated 7-ACA compound and, optionally, acylating the free amino group and/or substituting the 3' acetate group with a side chain suitable to form a cephalosporin antibiotic, characterised in that the nucleotide sequence encoding the acetyltransferase is derived from Acremonium chrysogenum and starts at the second ATG of the open reading frame as present in said nucleotide sequence.
PCT/EP1999/010292 1998-12-22 1999-12-21 Improved in vivo production of cephalosporins WO2000037671A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
KR1020017007915A KR20010089672A (en) 1998-12-22 1999-12-21 Improved in vivo production of cephalosporins
EP99964657A EP1141372A2 (en) 1998-12-22 1999-12-21 Improved in vivo production of cephalosporins
JP2000589724A JP2002533092A (en) 1998-12-22 1999-12-21 Improved in vivo production of cephalosporins
AU30426/00A AU3042600A (en) 1998-12-22 1999-12-21 Improved (in vivo) production of cephalosporins
HK02103286.5A HK1041610A1 (en) 1998-12-22 2002-05-02 Improved in vivo production of cephalosporins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98204469.5 1998-12-22
EP98204469 1998-12-22

Publications (2)

Publication Number Publication Date
WO2000037671A2 WO2000037671A2 (en) 2000-06-29
WO2000037671A3 true WO2000037671A3 (en) 2000-09-14

Family

ID=8234568

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/010292 WO2000037671A2 (en) 1998-12-22 1999-12-21 Improved in vivo production of cephalosporins

Country Status (7)

Country Link
EP (1) EP1141372A2 (en)
JP (1) JP2002533092A (en)
KR (1) KR20010089672A (en)
CN (1) CN1331751A (en)
AU (1) AU3042600A (en)
HK (1) HK1041610A1 (en)
WO (1) WO2000037671A2 (en)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004113508A1 (en) * 2003-06-20 2004-12-29 Dsm Ip Assets B.V. Production of cephalosporin intermediates in penicillium chrysogenum
CN101978063B (en) 2007-11-20 2018-10-16 帝斯曼知识产权资产管理有限公司 Succinic acid is produced in eukaryocyte
US20110318795A1 (en) 2007-11-20 2011-12-29 Verwaal Rene Dicarboxylic acid production in a filamentous fungus
CN101896606B (en) 2007-11-20 2016-12-21 帝斯曼知识产权资产管理有限公司 Dicarboxylic acids is produced in recombination yeast
CN101555463B (en) * 2008-04-08 2012-05-30 中国科学院上海生命科学研究院湖州工业生物技术中心 Recombinant escherichia coli strain expressing cephalosporin deacetylase and construction method thereof
EP2116136A1 (en) 2008-05-08 2009-11-11 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Novel phytases
WO2010107310A1 (en) 2009-03-17 2010-09-23 Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek Tno Novel cellulose and ligno-cellulose active proteins
EP2419518A1 (en) 2009-04-15 2012-02-22 DSM IP Assets B.V. Dicarboxylic acid production process
UA108853C2 (en) 2009-07-10 2015-06-25 Galactose fermentation method
WO2011131674A1 (en) 2010-04-21 2011-10-27 Dsm Ip Assets B.V. Process for the production of cells which are capable of converting arabinose
EP2388331A1 (en) 2010-05-21 2011-11-23 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Constitutive promoter
BR112013008981A2 (en) 2010-10-13 2017-10-03 Dsm Ip Assets Bv Yeast cell that ferments pentoses and glucose
EA201301193A1 (en) 2011-04-22 2014-02-28 ДСМ АйПи АССЕТС Б.В. Yeast cells capable of converting sugars containing arabinose and xylose
EP2554668A1 (en) 2011-08-04 2013-02-06 DSM IP Assets B.V. A pentose sugar fermenting cell
AR087423A1 (en) 2011-08-04 2014-03-19 Dsm Ip Assets Bv CAPABLE CELL TO FERMENT PENTOUS SUGARS
US20150031868A1 (en) 2012-01-23 2015-01-29 Dsm Ip Assets B.V. Diterpene production
IN2015DN02650A (en) 2012-10-16 2015-09-18 Dsm Ip Assets Bv
EP2772545A1 (en) 2013-03-01 2014-09-03 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Starch active proteins
US20160060660A1 (en) 2013-04-05 2016-03-03 Université Du Luxembourg Biotechnological production of itaconic acid
WO2014178717A1 (en) 2013-05-02 2014-11-06 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Novel organic acid pathway
EP3502264A3 (en) 2013-05-31 2019-11-06 DSM IP Assets B.V. Microorganisms for diterpene production
WO2015007748A1 (en) 2013-07-15 2015-01-22 Dsm Ip Assets B.V. Diterpene production
BR112016002092B1 (en) 2013-07-31 2020-12-15 Dsm Ip Assets B.V. STEVIOL GLYCOSIDE RECOVERY
EP3259361A1 (en) 2015-02-16 2017-12-27 DSM IP Assets B.V. Process for producing itaconic acid under anaerobic conditions
EP3862426A3 (en) 2015-03-16 2021-11-17 DSM IP Assets B.V. Udp-glycosyltransferases
CA2979957A1 (en) 2015-03-23 2016-09-29 Dsm Ip Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
EP3718417A1 (en) 2015-04-03 2020-10-07 DSM IP Assets B.V. Steviol glycosides
WO2016172282A1 (en) 2015-04-21 2016-10-27 Dsm Ip Assets B.V. Microbial production of terpenoids
PL3298133T3 (en) 2015-05-22 2020-03-31 Dsm Ip Assets B.V. Acetate consuming yeast cell
BR112018000550B1 (en) 2015-07-10 2022-05-10 Dsm Ip Assets B.V. Method for preparing a steviol glycoside composition
CN107922913B (en) 2015-08-13 2022-04-05 帝斯曼知识产权资产管理有限公司 Steviol glycoside transport
WO2017050652A1 (en) 2015-09-25 2017-03-30 Dsm Ip Assets B.V. Asparaginase
EP4043558A3 (en) 2015-10-05 2022-11-16 DSM IP Assets B.V. Kaurenoic acid hydroxylases
WO2017060195A1 (en) 2015-10-06 2017-04-13 Dsm Ip Assets B.V. Eukaryotic cell with increased production of fermentation product
CA3009871A1 (en) 2015-12-30 2017-07-06 Dsm Ip Assets B.V. Partial enzymatic hydrolysis of triacylglycerols
CN109153979A (en) 2015-12-30 2019-01-04 帝斯曼知识产权资产管理有限公司 The partial enzymatic hydrolysis of triacylglycerol
WO2017167847A1 (en) 2016-03-31 2017-10-05 Dsm Ip Assets B.V. Production of milk with low lactose content
EP3435772A1 (en) 2016-03-31 2019-02-06 DSM IP Assets B.V. Enzyme composition and preparation of a dairy product with improved properties
EP3435771A1 (en) 2016-03-31 2019-02-06 DSM IP Assets B.V. Enzyme composition and preparation of a dairy product with improved properties
WO2017216136A1 (en) 2016-06-14 2017-12-21 Dsm Ip Assets B.V. Recombinant yeast cell
EP3919615A1 (en) 2016-07-13 2021-12-08 DSM IP Assets B.V. Malate dehyrogenases
BR112019002417B1 (en) 2016-08-09 2023-05-02 Dsm Ip Assets B.V. CRYSTALLIZATION OF STEVIOL GLYCOSIDES
EP3497110A1 (en) 2016-08-09 2019-06-19 DSM IP Assets B.V. Crystallization of steviol glycosides
CN110036114B (en) 2016-08-26 2023-09-29 勒萨弗尔公司 Enhanced itaconic acid production
KR101808192B1 (en) * 2016-08-26 2018-01-18 아미코젠주식회사 Methods for preparing recombinant Acremonium chrysogenum producing 7-aminocephalosporanic acid with high concentration and Acremonium chrysogenum prepared thereby
WO2018073107A1 (en) 2016-10-19 2018-04-26 Dsm Ip Assets B.V. Eukaryotic cell comprising xylose isomerase
US11225647B2 (en) 2016-10-27 2022-01-18 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthases
JP2020500520A (en) 2016-12-08 2020-01-16 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． Kaurenate hydroxylase
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114941A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
BR112019012559A2 (en) 2016-12-21 2019-11-26 Dsm Ip Assets Bv lipolytic enzyme variants
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
EP3638770B1 (en) 2017-06-13 2023-01-18 DSM IP Assets B.V. Recombinant yeast cell
US11639497B2 (en) 2017-06-27 2023-05-02 Dsm Ip Assets B.V. UDP-glycosyltransferases
CA3074001A1 (en) 2017-09-26 2019-04-04 Dsm Ip Assets B.V. Acetic acid consuming strain
EP3533878A1 (en) 2018-02-28 2019-09-04 Dutch DNA Biotech B.V. Process for producing citramalic acid employing aspergillus
EP3809852A1 (en) 2018-06-19 2021-04-28 DSM IP Assets B.V. Lipolytic enzyme variants
EP4232568A1 (en) 2020-10-22 2023-08-30 DSM IP Assets B.V. Microorganisms for diterpene production
EP4359426A2 (en) 2021-06-24 2024-05-01 Fonterra Co-Operative Group Limited Recombinant beta-lactoglobulin
EP4370690A1 (en) 2021-07-12 2024-05-22 Danisco Us Inc Recombinant yeast cell
EP4370692A1 (en) 2021-07-12 2024-05-22 Danisco Us Inc Recombinant yeast cell
WO2023285282A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
WO2023285279A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
EP4370689A1 (en) 2021-07-12 2024-05-22 Danisco Us Inc Recombinant yeast cell
WO2023079048A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Process for the production of ethanol and recombinant yeast cell
WO2023079049A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Variant polypeptide and recombinant yeast cell
WO2023079050A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Recombinant yeast cell
CA3237662A1 (en) 2021-11-09 2023-05-19 Amgen Inc. Production of therapeutic proteins
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993008287A1 (en) * 1991-10-15 1993-04-29 Merck & Co., Inc. Novel bioprocesses for preparing 7-aca and 7-adac
EP0566897A2 (en) * 1992-04-07 1993-10-27 Hoechst Aktiengesellschaft The complete gene (cefG) encoding the acetyl-CoA: deacetylcephalosporin C acetyltransferase of Cephalosporium acremonium, its isolation and use
WO1995004149A1 (en) * 1993-07-30 1995-02-09 Gist-Brocades B.V. Process for the efficient production of 7-adca via 3-(carboxyethylthio)propionyl-7-adca
WO1995004148A1 (en) * 1993-07-30 1995-02-09 Gist-Brocades B.V. Process for the efficient production of 7-adca via 2-(carboxyethylthio)acetyl-7-adca and 3-(carboxymethylthio)propionyl-7-adca
WO1998048035A1 (en) * 1997-04-22 1998-10-29 Gist-Brocades B.V. Process for the fermentative production of deacylated cephalosporins

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993008287A1 (en) * 1991-10-15 1993-04-29 Merck & Co., Inc. Novel bioprocesses for preparing 7-aca and 7-adac
EP0566897A2 (en) * 1992-04-07 1993-10-27 Hoechst Aktiengesellschaft The complete gene (cefG) encoding the acetyl-CoA: deacetylcephalosporin C acetyltransferase of Cephalosporium acremonium, its isolation and use
WO1995004149A1 (en) * 1993-07-30 1995-02-09 Gist-Brocades B.V. Process for the efficient production of 7-adca via 3-(carboxyethylthio)propionyl-7-adca
WO1995004148A1 (en) * 1993-07-30 1995-02-09 Gist-Brocades B.V. Process for the efficient production of 7-adca via 2-(carboxyethylthio)acetyl-7-adca and 3-(carboxymethylthio)propionyl-7-adca
WO1998048035A1 (en) * 1997-04-22 1998-10-29 Gist-Brocades B.V. Process for the fermentative production of deacylated cephalosporins

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GUTIÉRREZ S ET AL.: "Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 48, no. 5, November 1997 (1997-11-01), pages 606 - 614, XP000907542 *
VELASCO J ET AL.: "Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits", BIOCHEMICAL JOURNAL, vol. 337, no. 3, 1 February 1999 (1999-02-01), pages 379 - 385, XP002139191 *
VELASCO J ET AL.: "Molecular characterization of the cefG gene product of Acremonium chrysogenum", FUNGAL GENETICS - FOURTH EUROPEAN CONFERENCE ON FUNGAL GENETICS, April 1998 (1998-04-01), pages 193, XP002139190 *

Also Published As

Publication number Publication date
AU3042600A (en) 2000-07-12
KR20010089672A (en) 2001-10-08
HK1041610A1 (en) 2002-07-12
WO2000037671A2 (en) 2000-06-29
JP2002533092A (en) 2002-10-08
EP1141372A2 (en) 2001-10-10
CN1331751A (en) 2002-01-16

Similar Documents

Publication Publication Date Title
WO2000037671A3 (en) Improved in vivo production of cephalosporins
CA2040707A1 (en) Cephalosporin acetylhydrolase gene and protein encoded by said gene
RU2208644C2 (en) Method for preparing n-deacylated cephalosporin
RU99101488A (en) The method of enzymatic production of deacylated cephalosporins
CA2080573A1 (en) Novel bioprocesses for preparing 7-aca and 7-adac
MXPA04006304A (en) Process for the preparation of a beta-lactam antibiotic with mutated penicillin acylase.
JP2001527381A (en) Improved immobilized penicillin G acylase
EP0842937A3 (en) Process for the preparation of the cephalosporin derivatives cefotaxime and ceftriaxone
US5654425A (en) Method for the acylation of the 7-amino group of the cephalosporanic ring
CN1795197B (en) Cephem compound
JP2000512512A (en) Method for producing deacylated cephalosporin by fermentation
EP0638649B1 (en) New use of alcaligenes faecalis penicillin G acylase
HU9301188D0 (en) An improved method for producing cephalosporines
ATE244248T1 (en) PRODUCTION OF AMINO CEPHALOSPORANIC ACID DERIVATIVES
WO1998002567A3 (en) PROCESS FOR THE PREPARATION OF CEPHALOSPORINS USING $i(ACREMONIUM CHRYSOGENUM)
EP2176270B1 (en) Compositions comprising beta lactam compounds
EP1186668A1 (en) An enzymatic process for preparing Beta-lactam compounds
김경자 et al. Biosynthesis of Penicillins and Cephalosporins Antibiotics
CS209853B2 (en) Method of making the semisynthetic cephalosporine
MXPA98000406A (en) An acilase of penicillin g immobilized, better
BG51517A1 (en) Cephem derivatives of 5-carboxy-2-benzoxazolon and a method for their production
EP0815256A1 (en) Process for the preparation of a beta-lactam antibiotic
KR20000057579A (en) Process for the production of penicillin g or v, cephalosporin g or v, and derivatives thereof
MY119921A (en) Novel cephalosporins substituted at the 3-position by (methylhydrazono)- methyl groups and at the 7-amino group by a 2-(5-amino-1,2,4-thiadiazol-3- yl)-2-(fluoromethoxyhydrozyimino)-acetyl group.
KR950029266A (en) Process for preparing cepradine monohydrate

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 99814782.6

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 1999964657

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 1020017007915

Country of ref document: KR

Ref document number: IN/PCT/2001/00753/MU

Country of ref document: IN

ENP Entry into the national phase

Ref document number: 2000 589724

Country of ref document: JP

Kind code of ref document: A

WWP Wipo information: published in national office

Ref document number: 1020017007915

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: 09869404

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 1999964657

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 1999964657

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1020017007915

Country of ref document: KR