IN2015DN02650A - - Google Patents

Download PDF

Info

Publication number
IN2015DN02650A
IN2015DN02650A IN2650DEN2015A IN2015DN02650A IN 2015DN02650 A IN2015DN02650 A IN 2015DN02650A IN 2650DEN2015 A IN2650DEN2015 A IN 2650DEN2015A IN 2015DN02650 A IN2015DN02650 A IN 2015DN02650A
Authority
IN
India
Prior art keywords
polypeptide
seq
heterologous
sugar
identity
Prior art date
Application number
Inventor
Maria Bernedina Elizabeth; Jonkers
Paul; Klaassen
Aloysius Wilhelmus Rudolphus Hubertus; Teunissen
Jong Rene Marcel; De
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of IN2015DN02650A publication Critical patent/IN2015DN02650A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y404/00—Carbon-sulfur lyases (4.4)
    • C12Y404/01—Carbon-sulfur lyases (4.4.1)
    • C12Y404/01005—Lactoylglutathione lyase (4.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01001—Transketolase (2.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01002—Transaldolase (2.2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017—Xylulokinase (2.7.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03001—Ribulose-phosphate 3-epimerase (5.1.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01006—Ribose-5-phosphate isomerase (5.3.1.6)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)

Abstract

The invention relates to a cell capable of converting one or more pentose sugar and one or more hexose sugar into fermentation product constitutively expressing one or more heterologous or homologous polypeptide having the amino acid sequence set out in SEQ ID NO: 20, or a variant polypeptide thereof having at least 45% identity to SEQ ID NO 20. In an embodiment the heterologous polypeptide has glyoxalase activity.
IN2650DEN2015 2012-10-16 2013-10-15 IN2015DN02650A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12188715 2012-10-16
EP13166959 2013-05-08
PCT/EP2013/071462 WO2014060377A1 (en) 2012-10-16 2013-10-15 Cells with improved pentose conversion

Publications (1)

Publication Number Publication Date
IN2015DN02650A true IN2015DN02650A (en) 2015-09-18

Family

ID=49354680

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2650DEN2015 IN2015DN02650A (en) 2012-10-16 2013-10-15

Country Status (12)

Country Link
US (1) US10273507B2 (en)
EP (2) EP3492579A1 (en)
KR (1) KR20150070151A (en)
CN (2) CN112195145A (en)
AR (1) AR093025A1 (en)
BR (1) BR112015008370B1 (en)
DK (1) DK2909305T3 (en)
ES (1) ES2717682T3 (en)
IN (1) IN2015DN02650A (en)
MY (1) MY171218A (en)
PL (1) PL2909305T3 (en)
WO (1) WO2014060377A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10604773B2 (en) * 2012-10-16 2020-03-31 Dsm Ip Assets B.V. Cells with improved pentose conversion
US20170191087A1 (en) * 2014-05-26 2017-07-06 Vib Vzw Causative genes conferring acetic acid tolerance in yeast
BR112018002284A2 (en) 2015-08-05 2018-12-11 Cargill Inc fermentation method to produce a bioproduct, genetically engineered negative crabtree yeast and method to reduce glycerol production by an engineered yeast
JP6990385B2 (en) * 2017-05-16 2022-02-03 国立大学法人 長崎大学 Multiple gene transfer method
CN107937297B (en) * 2017-11-29 2021-04-20 大连理工大学 Multi-inhibitor stress tolerance saccharomyces cerevisiae, and preparation method and application thereof
CN111304105B (en) * 2020-02-27 2022-05-03 南京工业大学 Genetically engineered bacterium for producing lipase by using methanol and xylose cosubstrate and application thereof

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990014423A1 (en) 1989-05-18 1990-11-29 The Infergene Company Microorganism transformation
DE69033633T2 (en) 1989-07-07 2001-05-03 Unilever Nv Process for the production of a protein by means of a mushroom transformed by multi-copy integration of an expression vector
FR2679920A1 (en) 1991-08-02 1993-02-05 Rhone Poulenc Rorer Sa Highly stable recombinant yeasts for the production of recombinant proteins, their preparation and their use
ATE238425T1 (en) 1993-07-23 2003-05-15 Dsm Nv SELECTION MARKER GENE-FREE RECOMBINANT STRAINS: METHOD FOR THEIR PRODUCTION AND THE USE OF THESE STRAINS
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
WO1998046772A2 (en) 1997-04-11 1998-10-22 Dsm N.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
MXPA00011223A (en) 1998-05-19 2002-04-17 Dsm Nv Improved in vivo.
WO2000037671A2 (en) 1998-12-22 2000-06-29 Dsm N.V. Improved in vivo production of cephalosporins
BR0306740A (en) 2002-01-23 2004-12-28 Royal Nedalco B V Host cell transformed with a nucleic acid construct, isolated nucleic acid molecule, and processes for producing ethanol, and a fermentation product
EP1476520A1 (en) 2002-01-25 2004-11-17 Akzo Nobel N.V. Method and apparatus for gluing
SE0202090D0 (en) 2002-05-08 2002-07-04 Forskarpatent I Syd Ab A modified yeast consuming L-arabinose
US7232664B2 (en) 2002-05-30 2007-06-19 Natureworks Llc Fermentation process using specific oxygen uptake rates as a process control
WO2004085627A1 (en) 2003-03-26 2004-10-07 Forskarpatent I Syd Ab New saccharomyces cerevisiae strains utilizing xylose
CN101914462B (en) * 2004-07-16 2013-04-24 Dsm知识产权资产有限公司 Eukaryotic cell metabolic engineering of xylose fermentation
EP2410048B1 (en) 2005-01-24 2016-08-10 DSM IP Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
ES2534282T3 (en) 2006-06-29 2015-04-21 Dsm Ip Assets B.V. A method to achieve improved polypeptide expression
AU2007302867B2 (en) 2006-10-02 2013-11-07 Dsm Ip Assets B.V. Metabolic engineering of arabinose-fermenting yeast cells
WO2008086124A1 (en) 2007-01-03 2008-07-17 Vialogy Llc Multi parallax exploitation for omni-directional imaging electronic eye
US20100159546A1 (en) 2007-03-30 2010-06-24 Aristos Aristidou Metabolic engineering of yeasts for the production of 1-butanol
US20100304454A1 (en) 2007-07-19 2010-12-02 Royal Nedalco B.V. Novel arabinose-fermenting eukaryotic cells
WO2009099044A1 (en) 2008-02-04 2009-08-13 Toray Industries, Inc. Method of producing lactic acid by continuous fermentation
AU2009221104B2 (en) * 2008-03-07 2014-07-31 Dsm Ip Assets B.V. A pentose sugar fermenting cell
WO2009109634A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
DE102008029302B4 (en) 2008-06-19 2016-08-11 Insilico Biotechnology Ag Biotechnological production of acrylic acid
FR2934264B1 (en) 2008-07-22 2012-07-20 Arkema France MANUFACTURE OF VINYL ESTERS FROM RENEWABLE MATERIALS, VINYL ESTERS OBTAINED AND USES THEREOF
WO2012015949A2 (en) * 2010-07-29 2012-02-02 Joule Unlimited Technologies, Inc. Methods and compositions for improving yields of reduced products of photosynthetic microorganisms
PL2663645T3 (en) 2010-11-18 2015-05-29 Dsm Ip Assets Bv Yeast strains engineered to produce ethanol from glycerol
WO2014003555A1 (en) * 2012-06-27 2014-01-03 Rijksuniversiteit Groningen Improved penicillin production

Also Published As

Publication number Publication date
US20150275235A1 (en) 2015-10-01
AR093025A1 (en) 2015-05-13
CN104718280A (en) 2015-06-17
DK2909305T3 (en) 2019-04-15
WO2014060377A1 (en) 2014-04-24
EP2909305B1 (en) 2018-12-26
BR112015008370A2 (en) 2021-06-15
CN112195145A (en) 2021-01-08
ES2717682T3 (en) 2019-06-24
EP3492579A1 (en) 2019-06-05
BR112015008370B1 (en) 2022-10-18
KR20150070151A (en) 2015-06-24
US10273507B2 (en) 2019-04-30
PL2909305T3 (en) 2019-06-28
EP2909305A1 (en) 2015-08-26
MY171218A (en) 2019-10-02

Similar Documents

Publication Publication Date Title
IN2015DN02650A (en)
MX2020010399A (en) 3-epimerase.
GB2540694A (en) CCR5 disruption of cells expressing anti-hiv chimeric antigen receptor (CAR) derived from broadly neutralizing antibodies
CA2860107C (en) Differentiation of human embryonic stem cells into single hormonal insulin positive cells
MY187542A (en) Carbohydrate degrading polypeptide and uses thereof
UA116980C2 (en) Glyphosate resistant plants and associated methods
EP4276180A3 (en) Clostridium histolyticum enzyme
MX2012015142A (en) Polypeptide having beta-glucosidase activity and uses thereof.
MY160897A (en) Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof
IN2015MN00436A (en)
IN2014CN01706A (en)
MX2016009149A (en) Improved enzyme variants of lactase from kluyveromyces lactis.
WO2014024090A3 (en) Fungal resistant plants expressing hcp5
IN2015DN03206A (en)
MX342171B (en) Peptide or peptide complex binding to 2 integrin and methods and uses involving the same.
MX2015015274A (en) Novel organic acid pathway.
WO2011141613A3 (en) Novel phytase, method for obtaining same and use thereof
MY185050A (en) A pentose sugar fermenting cell
MX2015001542A (en) Method.
WO2012094193A3 (en) Methods for enhancing the delivery of gene-transduced cells
WO2011159092A3 (en) Novel soil microorganism, novel oxidoreductase separated from the soil microorganism, gene encoding the oxidoreductase, and method for producing aglycones using the microorganism, the oxidoreductase and the gene
NZ709120A (en) Beta-hexosyl-transferases and uses thereof
IN2015DN01042A (en)
WO2012125720A3 (en) Polypeptides having immunoactivating activity and methods of producing the same
EA201300059A1 (en) POLYPEPTID, possessing the activity of the Volvolenin, and its application