Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
  • C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
  • A61K31/445—Non condensed piperidines, e.g. piperocaine
  • A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
  • A61K31/4545—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a six-membered ring with nitrogen as a ring hetero atom, e.g. pipamperone, anabasine
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/47—Quinolines; Isoquinolines
  • A61K31/472—Non-condensed isoquinolines, e.g. papaverine
  • A61K31/4725—Non-condensed isoquinolines, e.g. papaverine containing further heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/4965—Non-condensed pyrazines
  • A61K31/497—Non-condensed pyrazines containing further heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/50—Pyridazines; Hydrogenated pyridazines
  • A61K31/501—Pyridazines; Hydrogenated pyridazines not condensed and containing further heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
  • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
  • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
  • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
  • A61K31/5375—1,4-Oxazines, e.g. morpholine
  • A61K31/5386—1,4-Oxazines, e.g. morpholine spiro-condensed or forming part of bridged ring systems
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
  • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
  • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
  • A61K47/545—Heterocyclic compounds
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
  • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
  • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
  • A61K47/55—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound the modifying agent being also a pharmacologically or therapeutically active agent, i.e. the entire conjugate being a codrug
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
  • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
  • C07D487/04—Ortho-condensed systems
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D513/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
  • C07D513/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains two hetero rings
  • C07D513/04—Ortho-condensed systems
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D513/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00
  • C07D513/12—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for in groups C07D463/00, C07D477/00 or C07D499/00 - C07D507/00 in which the condensed system contains three hetero rings
  • C07D513/14—Ortho-condensed systems
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D519/00—Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00

Definitions

• the present invention relates to compounds and methods useful for the modulation of one or more interleukin- 1 receptor-associated kinases (“IRAK”) via ubiquitination and/or degradation by compounds according to the present invention.
• IRAK interleukin- 1 receptor-associated kinases
• the invention also provides pharmaceutically acceptable compositions comprising compounds of the present invention and methods of using said compositions in the treatment of various disorders.
• Ubiquitin-Proteasome Pathway is a critical pathway that regulates key regulator proteins and degrades misfolded or abnormal proteins. UPP is central to multiple cellular processes, and if defective or imbalanced, it leads to pathogenesis of a variety of diseases. The covalent attachment of ubiquitin to specific protein substrates is achieved through the action of E3 ubiquitin ligases.
• E3 ubiquitin ligases which facilitate the ubiquitination of different proteins in vivo, which can be divided into four families: HECT-domain E3s, U-box E3s, monomeric RING E3s and multi-subunit E3s. See generally Li et al. (PLOS One, 2008, 3, 1487) titled “Genome-wide and functional annotation of human E3 ubiquitin ligases identifies MULAN, a mitochondrial E3 that regulates the organelle’s dynamics and signaling.”; Bemdsen et al. (Nat. Struct. Mol.
• UPP plays a key role in the degradation of short-lived and regulatory proteins important in a variety of basic cellular processes, including regulation of the cell cycle, modulation of cell surface receptors and ion channels, and antigen presentation.
• the pathway has been implicated in several forms of malignancy, in the pathogenesis of several genetic diseases (including cystic fibrosis, Angelman’s syndrome, and Liddle syndrome), in immune surveillance/viral pathogenesis, and in the pathology of muscle wasting.
• Many diseases are associated with an abnormal UPP and negatively affect cell cycle and division, the cellular response to stress and to extracellular modulators, morphogenesis of neuronal networks, modulation of cell surface receptors, ion channels, the secretory pathway, DNA repair and biogenesis of organelles.
• the UPP is used to induce selective protein degradation, including use of fusion proteins to artificially ubiquitinate target proteins and synthetic small-molecule probes to induce proteasome- dependent degradation.
• Bifunctional compounds composed of a target protein-binding ligand and an E3 ubiquitin ligase ligand, induced proteasome-mediated degradation of selected proteins via their recruitment to E3 ubiquitin ligase and subsequent ubiquitination. These drug-like molecules offer the possibility of temporal control over protein expression.
• Such compounds are capable of inducing the inactivation of a protein of interest upon addition to cells or administration to an animal or human, and could be useful as biochemical reagents and lead to a new paradigm for the treatment of diseases by removing pathogenic or oncogenic proteins (Crews C, Chemistry & Biology, 2010, 17(6):551-555; Schnnekloth JS Jr., Chembiochem, 2005, 6(1): 40-46).
• the present application relates novel bifunctional compounds, which function to recruit IRAK kinases to E3 Ubiquitin Ligase for degradation, and methods of preparation and uses thereof.
• the present disclosure provides bifunctional compounds, which find utility as modulators of targeted ubiquitination of IRAK kinases, which are then degraded and/or otherwise inhibited by the bifunctional compounds as described herein.
• An advantage of the compounds provided herein is that a broad range of pharmacological activities is possible, consistent with the degradation/inhibition of IRAK kinases.
• the description provides methods of using an effective amount of the compounds as described herein for the treatment or amelioration of a disease condition, such as cancer, e.g., multiple myeloma.
• the present application further relates to targeted degradation of IRAK kinases through the use of bifimctional molecules, including bifimctional molecules that link a degradation inducing moiety to a ligand that binds IRAK kinases having the following general formula I:
• the present invention further relates to bifimctional compounds that not only degrade IRAK, but also degrade IMiD substrates, such as Ikaros, Aiolos, or Ikaros and Aiolos.
• Compounds of the present invention are useful for treating a variety of diseases, disorders or conditions, associated with regulation of signaling pathways implicating IRAK kinases. Such diseases, disorders, or conditions include those described herein.
• Compounds provided by this invention are also useful for the study of IRAK enzymes in biological and pathological phenomena; the study of intracellular signal transduction pathways occurring in bodily tissues; and the comparative evaluation of new IRAK inhibitors or IRAK degraders or other regulators of kinases, signaling pathways, and cytokine levels in vitro or in vivo.
• Compounds of the present invention, and compositions thereof, are useful as degraders and/or inhibitors of one or more IRAK protein kinases.
• a provided compound degrades and/or inhibits IRAK-1/2/3/4.
• a provided compound degrades IRAK4 and IMiD substrates, such as Ikaros, Aiolos, or Ikaros and Aiolos.
• the present invention provides a compound of formula I:
• IRAK is an IRAK binding moiety capable of binding to one or more of IRAKI, IRAK2, IRAK3, and IRAK4;
• L is a bivalent moiety that connects IRAK to LBM; and LBM is a cereblon E3 ubiquitin ligase binding moiety.
• the present invention provides a compound of formula I:
• IRAK is an IRAK binding moiety capable of binding to IRAK4;
• L is a bivalent moiety that connects IRAK to LBM
• LBM is a IMiD-based cereblon E3 ubiquitin ligase binding moiety.
• aliphatic or “aliphatic group”, as used herein, means a straight-chain (i.e., unbranched) or branched, substituted or unsubstituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation, or a monocyclic hydrocarbon or bicyclic hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic (also referred to herein as "carbocycle,” “cycloaliphatic” or “cycloalkyl”), that has a single point of attachment to the rest of the molecule.
• aliphatic groups contain 1-6 aliphatic carbon atoms.
• aliphatic groups contain 1-5 aliphatic carbon atoms. In other embodiments, aliphatic groups contain 1-4 aliphatic carbon atoms. In still other embodiments, aliphatic groups contain 1-3 aliphatic carbon atoms, and in yet other embodiments, aliphatic groups contain 1-2 aliphatic carbon atoms.
• “cycloaliphatic” (or “carbocycle” or “cycloalkyl”) refers to a monocyclic C3-C6 hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule.
• Suitable aliphatic groups include, but are not limited to, linear or branched, substituted or unsubstituted alkyl, alkenyl, alkynyl groups and hybrids thereof such as (cycloalkyl)alkyl, (cycloalkenyl)alkyl or (cycloalkyl)alkenyl.
• bridged bicyclic refers to any bicyclic ring system, i.e. carbocyclic or heterocyclic, saturated or partially unsaturated, having at least one bridge.
• a “bridge” is an unbranched chain of atoms or an atom or a valence bond connecting two bridgeheads, where a “bridgehead” is any skeletal atom of the ring system which is bonded to three or more skeletal atoms (excluding hydrogen).
• a bridged bicyclic group has 7-12 ring members and 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• bridged bicyclic groups are well known in the art and include those groups set forth below where each group is attached to the rest of the molecule at any substitutable carbon or nitrogen atom. Unless otherwise specified, a bridged bicyclic group is optionally substituted with one or more substituents as set forth for aliphatic groups. Additionally or alternatively, any substitutable nitrogen of a bridged bicyclic group is optionally substituted. Exemplary bridged bicyclics include:
• lower alkyl refers to a C H straight or branched alkyl group.
• exemplary lower alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, isobutyl, and tert-butyl.
• lower haloalkyl refers to a Cw straight or branched alkyl group that is substituted with one or more halogen atoms.
• heteroatom means one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quatemized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3.4-dihydro-2 /-pyrrolyl). NH (as in pyrrolidinyl) or NR + (as in N-substituted pyrrolidinyl)).
• Ci-s saturated or unsaturated, straight or branched, hydrocarbon chain
• bivalent Ci-s (or Ci-e) saturated or unsaturated, straight or branched, hydrocarbon chain refers to bivalent alkylene, alkenylene, and alkynylene chains that are straight or branched as defined herein.
• alkylene refers to a bivalent alkyl group.
• An “alkylene chain” is a polymethylene group, i.e., -(CH2) n -, wherein n is a positive integer, preferably from 1 to 6, from 1 to 4, from 1 to 3, from 1 to 2, or from 2 to 3.
• a substituted alkylene chain is a polymethylene group in which one or more methylene hydrogen atoms are replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group.
• alkenylene refers to a bivalent alkenyl group.
• a substituted alkenylene chain is a polymethylene group containing at least one double bond in which one or more hydrogen atoms are replaced with a substituent. Suitable substituents include those described below for a substituted aliphatic group.
• cyclopropylenyl refers to a bivalent cyclopropyl group of the following structure:
• halogen means F, Cl, Br, or I.
• aryl used alone or as part of a larger moiety as in “aralkyl,” “aralkoxy,” or
• aryloxyalkyl refers to monocyclic or bicyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic and wherein each ring in the system contains 3 to 7 ring members.
• aryl may be used interchangeably with the term “aryl ring.”
• aryl refers to an aromatic ring system which includes, but not limited to, phenyl, biphenyl, naphthyl, anthracyl and the like, which may bear one or more substituents.
• aryl is a group in which an aromatic ring is fused to one or more non-aromatic rings, such as indanyl, phthalimidyl, naphthimidyl, phenanthridinyl, or tetrahydronaphthyl, and the like.
• heteroaryl and “heteroar-,” used alone or as part of a larger moiety, e.g., “heteroaralkyl,” or “heteroaralkoxy,” refer to groups having 5 to 10 ring atoms, preferably 5, 6, or 9 ring atoms; having 6, 10, or 14 p electrons shared in a cyclic array; and having, in addition to carbon atoms, from one to five heteroatoms.
• heteroatom refers to nitrogen, oxygen, or sulfur, and includes any oxidized form of nitrogen or sulfur, and any quatemized form of a basic nitrogen.
• Heteroaryl groups include, without limitation, thienyl, furanyl, pyrrolyl, imidazolyl, pyrazolyl, triazolyl, tetrazolyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, isothiazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrimidinyl, pyrazinyl, indolizinyl, purinyl, naphthyridinyl, and pteridinyl.
• heteroaryl and “heteroar-”, as used herein, also include groups in which a heteroaromatic ring is fused to one or more aryl, cycloaliphatic, or heterocyclyl rings, where the radical or point of attachment is on the heteroaromatic ring.
• Nonlimiting examples include indolyl, isoindolyl, benzothienyl, benzofuranyl, dibenzofuranyl, indazolyl, benzimidazolyl, benzthiazolyl, quinolyl, isoquinolyl, cinnolinyl, phthalazinyl, quinazolinyl, quinoxalinyl, 4H quinolizinyl.
• heteroaryl group may be mono- or bicyclic.
• heteroaryl may be used interchangeably with the terms “heteroaryl ring,” “heteroaryl group,” or “heteroaromatic,” any of which terms include rings that are optionally substituted.
• heterooaralkyl refers to an alkyl group substituted by a heteroaryl, wherein the alkyl and heteroaryl portions independently are optionally substituted.
• heterocycle As used herein, the terms “heterocycle,” “heterocyclyl,” “heterocyclic radical,” and
• heterocyclic ring are used interchangeably and refer to a stable 5- to 7-membered monocyclic or 7—10— membered bicyclic heterocyclic moiety that is either saturated or partially unsaturated, and having, in addition to carbon atoms, one or more, preferably one to four, heteroatoms, as defined above.
• nitrogen includes a substituted nitrogen.
• the nitrogen may be N (as in 3.4-dihydro-2 / pyrrolyl). NH (as in pyrrolidinyl), or + NR (as in N substituted pyrrolidinyl).
• a heterocyclic ring can be attached to its pendant group at any heteroatom or carbon atom that results in a stable structure and any of the ring atoms can be optionally substituted.
• saturated or partially unsaturated heterocyclic radicals include, without limitation, tetrahydrofuranyl, tetrahydrothiophenyl pyrrolidinyl, piperidinyl, pyrrolinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, oxazolidinyl, piperazinyl, dioxanyl, dioxolanyl, diazepinyl, oxazepinyl, thiazepinyl, morpholinyl, and quinuclidinyl.
• heterocycle used interchangeably herein, and also include groups in which a heterocyclyl ring is fused to one or more aryl, heteroaryl, or cycloaliphatic rings, such as indolinyl, 3// indolyl. chromanyl, phenanthridinyl, or tetrahydroquinolinyl.
• a heterocyclyl group may be monocyclic, bicyclic, bridged bicyclic, or spirocyclic.
• heterocyclylalkyl refers to an alkyl group substituted by a heterocyclyl, wherein the alkyl and heterocyclyl portions independently are optionally substituted.
• partially unsaturated refers to a ring moiety that includes at least one double or triple bond.
• partially unsaturated is intended to encompass rings having multiple sites of unsaturation, but is not intended to include aryl or heteroaryl moieties, as herein defined.
• compounds of the invention may contain “optionally substituted” moieties.
• substituted means that one or more hydrogens of the designated moiety are replaced with a suitable substituent.
• an “optionally substituted” group may have a suitable substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position.
• Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds.
• stable refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, and, in certain embodiments, their recovery, purification, and use for one or more of the purposes disclosed herein.
• Suitable monovalent substituents on R° are independently halogen, -(CH 2 )o- 2 R*, - (haloR*), -(CH 2 ) O-2 OH, -(CH 2 ) O-2 OR # , -(CH 2 ) 0-2 CH(OR # ) 2 ; -0(haloR # ), -CN, -N 3 , -(CH 2 ) 0-2 C(O)R # , - (CH 2 ) O-2 C(0)OH, -(CH 2 ) O-2 C(0)OR ⁇ , -(CH 2 ) O-2 SR # , -(CH 2 ) O-2 SH, -(CH 2 ) O-2 NH 2 , -(CH 2 ) O-2 NHR ⁇ , - (CH 2 ) O-2 NR # 2 , -N0 2 , -SiR*
• R * NOR * , - 0(C(R * 2 )) 2-3 0-, or-S(C(R * 2 )) 2-3 S-, wherein each independent occurrence of R * is selected from hydrogen, Ci-6 aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• Suitable divalent substituents that are bound to vicinal substitutable carbons of an “optionally substituted” group include: -0(CR * 2 ) 2-3 0-, wherein each independent occurrence of R * is selected from hydrogen, C , aliphatic which may be substituted as defined below, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• Suitable substituents on the aliphatic group of R * include halogen, -R # , -(haloR*), -OH, -OR*, -0(haloR*), -CN, -C(0)OH, -C(0)OR*, -NH 2 , -NHR*, -NR* 2 , or -NO 3 ⁇ 4 wherein each R* is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently Ci- 4 aliphatic, -CH 2 Ph, -0(CH 2 )o-iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• Suitable substituents on a substitutable nitrogen of an “optionally substituted” group include - R ⁇ , -NR ⁇ 2 , -C(0)Rt, -C(0)ORt, -C(0)C(0)Rt, -C(0)CH 2 C(0)Rt, -S(0) 2 Rt, -S(0) 2 NRt 2 , -C(S)NRt 2 , - C(NH)NR ⁇ 2 , or -N(R ⁇ )S(0) 2 R ⁇ ; wherein each R ⁇ is independently hydrogen, Ci-e aliphatic which may be substituted as defined below, unsubstituted -OPh, or an unsubstituted 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or, notwithstanding the definition above, two independent occurrences of R ⁇ , taken together with their intervening atom(s) form an unsubstituted 3
• Suitable substituents on the aliphatic group of R ⁇ are independently halogen, -R*, -(haloR*), - OH, -OR*, -0(haloR # ), -CN, -C(0)OH, -C(0)OR # , -NH 2 , -NHR*, -NR* 2 .
• each R* is unsubstituted or where preceded by “halo” is substituted only with one or more halogens, and is independently Ci- 4 aliphatic, -CH 2 Ph, -0(CH 2 )o-iPh, or a 5-6-membered saturated, partially unsaturated, or aryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• the term “provided compound” refers to any genus, subgenus, and/or species set forth herein.
• the term "pharmaceutically acceptable salt” refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio.
• Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge et al., describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference.
• Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases.
• Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
• inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid
• organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
• salts include adipate, alginate, ascorbate, aspartate, benzene sulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methane sulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pect
• Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N (Ci-4alkyl) salts.
• Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like.
• Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate.
• structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, and geometric (or conformational)) forms of the structure; for example, the R and S configurations for each asymmetric center, Z and E double bond isomers, and Z and E conformational isomers. Therefore, single stereochemical isomers as well as enantiomeric, diastereomeric, and geometric (or conformational) mixtures of the present compounds are within the scope of the invention. Unless otherwise stated, all tautomeric forms of the compounds of the invention are within the scope of the invention.
• structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms.
• compounds having the present structures including the replacement of hydrogen by deuterium or tritium, or the replacement of a carbon by a 13 C- or 14 C-enriched carbon are within the scope of this invention.
• Such compounds are useful, for example, as analytical tools, as probes in biological assays, or as therapeutic agents in accordance with the present invention
• an inhibitor is defined as a compound that binds to and/or inhibits an IRAK kinase with measurable affinity.
• an inhibitor has an IC50 and/or binding constant of less than about 50 mM, less than about 1 mM, less than about 500 nM, less than about 100 nM, less than about 10 nM, or less than about 1 nM.
• the term “degrader” is defined as a heterobifunctional or monovalent compound that binds to and/or inhibits both an IRAK kinase and an E3 ligase with measurable affinity resulting in the ubiqitination and subsequent degradation of the IRAK kinase.
• a degrader has an DC50 of less than about 50 pM, less than about 1 pM, less than about 500 nM, less than about 100 nM, less than about 10 nM, or less than about 1 nM.
• the term “monovalent” refers to a degrader compound without an appended E3 ligase binding moiety.
• a compound of the present invention may be tethered to a detectable moiety. It will be appreciated that such compounds are useful as imaging agents.
• a detectable moiety may be attached to a provided compound via a suitable substituent.
• suitable substituent refers to a moiety that is capable of covalent attachment to a detectable moiety.
• moieties are well known to one of ordinary skill in the art and include groups containing, e.g., a carboxylate moiety, an amino moiety, a thiol moiety, or a hydroxyl moiety, to name but a few.
• moieties may be directly attached to a provided compound or via a tethering group, such as a bivalent saturated or unsaturated hydrocarbon chain.
• such moieties may be attached via click chemistry.
• such moieties may be attached via a 1,3 -cycloaddition of an azide with an alkyne, optionally in the presence of a copper catalyst.
• Methods of using click chemistry are known in the art and include those described by Rostovtsev etal, Angew. Chem. Int. Ed. 2002, 41, 2596-99 and Sun et al, Bioconjugate Chem., 2006, 17, 52-57.
• detectable moiety is used interchangeably with the term “label” and relates to any moiety capable of being detected, e.g., primary labels and secondary labels.
• Primary labels such as radioisotopes (e.g., tritium, 32 P, 33 P, 35 S, or 14 C), mass-tags, and fluorescent labels are signal generating reporter groups which can be detected without further modifications.
• Detectable moieties also include luminescent and phosphorescent groups.
• secondary label refers to moieties such as biotin and various protein antigens that require the presence of a second intermediate for production of a detectable signal.
• the secondary intermediate may include streptavidin-enzyme conjugates.
• antigen labels secondary intermediates may include antibody-enzyme conjugates.
• fluorescent label refers to moieties that absorb light energy at a defined excitation wavelength and emit light energy at a different wavelength.
• fluorescent labels include, but are not limited to: Alexa Fluor dyes (Alexa Fluor 350, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 568, Alexa Fluor 594, Alexa Fluor 633, Alexa Fluor 660 and Alexa Fluor 680), AMCA, AMCA-S, BODIPY dyes (BODIPY FF, BODIPY R6G, BODIPY TMR, BODIPY TR, BODIPY 530/550, BODIPY 558/568, BODIPY 564/570, BODIPY 576/589, BODIPY 581/591, BODIPY 630/650, BODIPY 650/665), Carboxyrhodamine 6G, carb
• mass-tag refers to any moiety that is capable of being uniquely detected by virtue of its mass using mass spectrometry (MS) detection techniques.
• mass-tags include electrophore release tags such as N-[3-[4’-[(p-Methoxytetrafluorobenzyl)oxy]phenyl]-3- methylglyceronyl]isonipecotic Acid, 4’-[2,3,5,6-Tetrafluoro-4-(pentafluorophenoxyl)]methyl acetophenone, and their derivatives. The synthesis and utility of these mass-tags is described in United
• mass-tags include, but are not limited to, nucleotides, dideoxynucleotides, oligonucleotides of varying length and base composition, oligopeptides, oligosaccharides, and other synthetic polymers of varying length and monomer composition.
• a large variety of organic molecules, both neutral and charged (biomolecules or synthetic compounds) of an appropriate mass range (100-2000 Daltons) may also be used as mass-tags.
• measurable affinity and “measurably inhibit,” as used herein, means a measurable change in an IRAK protein kinase activity between a sample comprising a compound of the present invention, or composition thereof, and an IRAK protein kinase, and an equivalent sample comprising an IRAK protein kinase, in the absence of said compound, or composition thereof.
• the present invention provides a compound of formula I:
• IRAK is an IRAK4 binding moiety
• L is a bivalent moiety that connects IRAK to LBM; and LBM is a cereblon E3 ubiquitin ligase binding moiety.
• IRAK IRAK Binding Moiety
• the present invention provides a compound of formula I, where IRAK is an IRAK4 binding moiety thereby forming a compound of formula I-a: or a pharmaceutically acceptable salt thereof, wherein DIM and L are as defined and described herein, and wherein: each R x is independently hydrogen, deuterium, R z , halogen, -CN, -NO2, -OR,
• N(R)C(0)0R, -N(R)C(0)R, -N(R)C(0)NR 2 , -N(R)S(0) 2 R, -0P(0)R 2 , -0P(0)(0R) 2 , - each R z is independently an optionally substituted group selected from Ci- 6 aliphatic, phenyl, a 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur;
• Ring Q is selected from benzo or a fused 5-6 membered heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur;
• Ring T is selected from phenyl, a 4-11 membered saturated or partially unsaturated monocyclic, bicyclic, bridged bicyclic, or spirocyclic carbocyclic or heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or a 5-10 membered monocyclic or bicyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein Ring T is further optionally substituted with 1-2 oxo groups;
• -Cy x - is an optionally substituted ring selected from a 3-5 membered saturated or partially unsaturated carbocyclic or heterocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or a 5 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein -Cy x - is optionally substituted with 1-2 oxo groups;
• X is a covalent bond or an optionally substituted bivalent ring selected from phenylenyl, a 4-11 membered saturated or partially unsaturated monocyclic, bicyclic, bridged bicyclic, or spirocyclic carbocyclylenyl or heterocyclylenyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or a 5-6 membered heteroarylenyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each x is 0, 1, 2, 3 or 4; and each y is 0, 1, 2, 3 or 4; wherein the compound of formula I-a is not compound 1-1 or 1-2 in Table 1A.
• each R x is independently hydrogen, deuterium, R z , halogen, -CN, -N0 2 , -OR, -SR, -NR 2 , -S(0) 2 R, -S(0) 2 NR 2 -S(0)R, -CFR 2 , -CF 2 R, -CF 3 , -CR 2 (OR), - CR 2 (NR 2 ), -C(0)R, -C(0)OR, -C(0)NR 2 , -C(0)N(R)OR, -OC(0)R, -OC(0)NR 2 , -
• 0P(0)(0R)NR 2 , -0P(0)(NR 2 ) 2 , -P(0)R 2 , -S1R3, -Si(OR)R 2 , or ; or two R x groups are optionally taken together to form an optionally substituted 5-7 membered partially unsaturated or aryl fused ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• each R x is independently hydrogen. In some embodiments, R x is deuterium. In some embodiments, each R x is independently R z . In some embodiments, each R x is independently halogen. In some embodiments, each R x is independently -CN. In some embodiments, each R x is independently -NO2. In some embodiments, each R x is independently -OR. In some embodiments, each R x is independently -SR. In some embodiments, each R x is independently -NR2. In some embodiments, each R x is independently -S(0) 2 R. In some embodiments, each R x is independently -S(0) 2 NR 2. In some embodiments, each R x is independently -S(0)R.
• each R x is independently -CFR2. In some embodiments, each R x is independently -CF2R. In some embodiments, each R x is independently -CF3. In some embodiments, each R x is independently -CR2(OR). In some embodiments, each R x is independently -CR 2 (NR 2 ). In some embodiments, each R x is independently -C(0)R. In some embodiments, each R x is independently -C(0)OR. In some embodiments, each R x is independently -C(0)NR 2 . In some embodiments, each R x is independently -N + (0 )R 2 . In some embodiments, each R x is independently -OP(0)R 2 .
• each R x is independently - OP(0)(OR) 2 . In some embodiments, each R x is independently -OP(0)(OR)NR 2 . In some embodiments, each R x is independently -0P(0)(NR 2 ) 2 . In some embodiments each R x is independently -P(0)R 2 . In some embodiments, each R x is independently -SiR 3 . In some embodiments, each R x is independently -Si(OR)R2. In some embodiments, each R x is independently -SF5. In some embodiments, each R x is independently
• two R x groups are optionally taken together to form an optionally substituted 5-6 membered partially unsaturated or aryl fused ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• two R x groups are optionally taken together to form an optionally substituted 3-5 membered saturated or partially unsaturated carbocyclic or heterocyclic spiro fused ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur,
• R x is In some embodiments, R x is -CF 2 H. In some embodiments, R x is -OMe. In some embodiments, R x is -Me. In some embodiments, R x is -OCF 2 H. In some embodiments, R x is -OCF3. In some embodiments, R x is In some embodiments, R x is . , . In some embodiments, R x is some embodiments,
• each R x is selected from those depicted in Table 1, below.
• each R is independently hydrogen, or an optionally substituted group selected from Ci-e aliphatic, phenyl, a 4-7 membered saturated or partially unsaturated heterocyclic having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or two R groups on the same carbon or nitrogen are optionally taken together with their intervening atoms to form a 4-7 membered saturated, partially unsaturated, or heteroaryl ring having 0-3 heteroatoms, in addition to the carbon or nitrogen, independently selected from nitrogen, oxygen, and sulfur.
• each R is independently hydrogen. In some embodiments, each R is an optionally substituted group selected from Ci-e aliphatic. In some embodiments, each R is an optionally substituted phenyl. In some embodiments, each R is an optionally substituted 4-7 membered saturated or partially unsaturated heterocyclic having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, each R is an optionally substituted 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.
• two R groups on the same nitrogen are optionally taken together with their intervening atoms to form an optionally substituted 4-7 membered saturated, partially unsaturated, or heteroaryl ring having 0-3 heteroatoms, in addition to the nitrogen, independently selected from nitrogen, oxygen, and sulfur.
• each R y is independently hydrogen, deuterium, R z , halogen, -CN, -NO2, -OR, -SR, -NR 2 , -S(0) 2 R, -S(0) 2 NR 2 -S(0)R, -CFR 2 , -CF 2 R, -CF 3 , -CR 2 (OR), - CR 2 (NR 2 ), -C(0)R, -C(0)OR, -C(0)NR 2 , -C(0)N(R)OR, -OC(0)R, -OC(0)NR 2 , -
• N(R)C(0)OR, -N(R)C(0)R, -N(R)C(0)NR 2 , -N(R)S(0) 2 R, -N + (0 )R 2 , -OP(0)R 2 , -OP(0)(OR) 2 , - two R y groups are optionally taken together to form an optionally substituted 5-6 membered partially unsaturated or aryl fused ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• each R y is independently hydrogen. In some embodiments, R y is deuterium. In some embodiments, each R y is independently R z . In some embodiments, each R y is independently halogen. In some embodiments, each R y is independently -CN. In some embodiments, each R y is independently -NO2. In some embodiments, each R y is independently -OR. In some embodiments, each R y is independently -SR. In some embodiments, each R y is independently -NR2. In some embodiments, each R y is independently -S(0) 2 R. In some embodiments, each R y is independently -S(0) 2 NR 2. In some embodiments, each R y is independently -S(0)R.
• each R y is independently -CFR2. In some embodiments, each R y is independently -CF2R. In some embodiments, each R y is independently -CF3. In some embodiments, each R y is independently -CR2(OR). In some embodiments, each R y is independently -CR2(NR2). In some embodiments, each R y is independently -C(0)R. In some embodiments, each R y is independently -C(0)OR. In some embodiments, each R y is independently -C(0)NR 2 . In some embodiments, each R y is independently -N (0 )R 2 . In some embodiments, each R y is independently -OP(0)R 2 .
• each R y is independently - OP(0)(OR) 2 . In some embodiments, each R y is independently -OP(0)(OR)NR 2 . In some embodiments, each R y is independently -0P(0)(NR 2 ) 2 . In some embodiments each R y is independently -P(0)R 2 . In some embodiments, each R y is independently -SiR3. In some embodiments, each R y is independently -Si(OR)R2. In some embodiments, each R y is independently -SF5. In some embodiments, each R y is independently
• two R y groups are optionally taken together to form an optionally substituted 5-7 membered partially unsaturated or aryl fused ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• R y is -CF2Me. In some embodiments, R y is -CFMe2. In some embodiments, R y is -Me. In some embodiments, R y is -OCF3. In some embodiments, R y is fluoro. In some embodiments,
• each R y is selected from those depicted in Table 1, below.
• each R z is independently an optionally substituted group selected from Ci- 6 aliphatic, phenyl, a 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.
• each R z is independently an optionally substituted group selected from Ci- 6 aliphatic. In some embodiments, each R z is independently an optionally substituted phenyl. In some embodiments, each R z is independently an optionally substituted 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, each R z is independently an optionally substituted 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. [0069] In some embodiments, each R z is selected from those depicted in Table 1, below.
• Ring Q is selected from benzo or a fused 5-6 membered heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur.
• Ring Q is benzo. In some embodiments, Ring Q is a fused 5-6 membered heteroaryl ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur.
• Ring Q is
• each Ring Q is selected from those depicted in Table 1, below.
• Ring T is selected from phenyl, a 4-11 membered saturated or partially unsaturated monocyclic, bicyclic, bridged bicyclic, or spirocyclic carbocyclic or heterocyclic ring 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or a 5-10 membered monocyclic or bicyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein Ring T is further optionally substituted with 1-2 oxo groups;
• Ring T is from phenyl. In some embodiments, Ring T is a 4-11 membered saturated or partially unsaturated monocyclic, bicyclic, bridged bicyclic, or spirocyclic carbocyclic or heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, Ring T is a 5-10 membered monocyclic or bicyclic heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, Ring T is further optionally substituted with 1-2 oxo groups. n some embodiments, Ring T is . In In some embodiments, Ring T is . In In some embodiments, Ring T is . In In some embodiments, Ring T is a 4-11 membered saturated or partially unsaturated monocyclic, bicyclic, bridged bicyclic, or spirocyclic carbocyclic or heterocyclic ring having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, Ring T is a
• Ring T is phenyl. In some embodiments, Ring T is . some embodiments, Ring T is
• Ring T is selected from those depicted in Table 1, below.
• L x is a covalent bond.
• L x is -C(0)N(H)-. In some embodiments, L x is -CH 2 C(0)N(H)-.
• L x combines with R y to form In some embodiments, L ! [0082] In some embodiments, Ring L x is selected from those depicted in Table 1, below.
• -Cy x - is an optionally substituted ring selected from a 3-5 membered saturated or partially unsaturated carbocyclic or heterocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or a 5 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, wherein -Cy x - is optionally substituted with 1-2 oxo groups.
• -Cy x - is an optionally substituted ring selected from a 3-5 membered saturated or partially unsaturated carbocyclic or heterocyclic ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, -Cy x - is a 5 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, -Cy x - is optionally substituted with 1-2 oxo groups.
• Ring -Cy x - is selected from those depicted in Table 1, below.
• X is a covalent bond or an optionally substituted bivalent ring selected from phenylenyl, a 4- 11 membered saturated or partially unsaturated monocyclic, bicyclic, bridged bicyclic, or spirocyclic carbocyclylenyl or heterocyclylenyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or a 5-6 membered heteroarylenyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.
• X is a covalent bond.
• X is an optionally substituted phenylenyl.
• X is an optionally substituted 4-11 membered saturated or partially unsaturated monocyclic, bicyclic, bridged bicyclic, or spirocyclic carbocyclylenyl or heterocyclylenyl having 1-3 heteroatoms independently selected from nitrogen, oxygen, and sulfur.
• X is an optionally substituted 5-6 membered heteroarylenyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.
• X is . In some embodiments, X is . In some embodiments,
• X is selected from those depicted in Table 1, below.
• each x and y are independently 0, 1, 2, 3 or 4.
• each x and y are independently 0. In some embodiments, each x and y are independently 1. In some embodiments, each x and y are independently 2. In some embodiments, each x and y are independently 3. In some embodiments, each x and y are independently 4.
• each x and y are selected from those depicted in Table 1, below.
• the present invention provides a compound of formula I-a, wherein Ring Q is benzo and Ring T is pyridinyl as shown, to provide a compound of formula I-a-1:
• the present invention provides a compound of formula I-a, wherein Ring Q is benzo and L x is an amide as shown, to provide a compound of formula I-a-2:
• the present invention provides a compound of formula I-a, wherein Ring Q is benzo and X is cyclohexyl as shown, to provide a compound of formula I-a-3:
• the present invention provides a compound of formula I-a, wherein Ring Q is benzo, X is cyclohexyl, and Ring T is pyridinyl as shown, to provide a compound of formula I- a-4:
• the present invention provides a compound of formula I-a, wherein Ring Q is benzo, X is cyclohexyl, and L x is an amide as shown, to provide a compound of formula I-a-5:
• IRAK is selected from those depicted in Table 1, below.
• LBM Ligase Binding Moiety
• E3 ligase ligand well known to one of ordinary skill in the art including those described in M. Toure, C. M. Crews, Angew. Chem. Int. Ed. 2016, 55, 1966, T. Uehara et al.
• UBM is a cereblon (CRBN) E3 ubiquitin ligase binding moiety.
• LBM is an IMiD-based cereblon E3 ubiquitin ligase binding moiety.
• an IMiD-based cereblon E3 ligase binding moiety includes thalidomide, lenalidomide, pomalidomide, avadomide (CC-122), iberdomide (CC-220), CC-92480, CC-885, CC-9009, and analogs thereof, and those IMiD-based cereblon ligands found in WO 2002059106, US 7,629,360, US 5,874,448, WO 2009145899, WO 2009042177, WO 1999047512, WO 2008039489, WO 2008115516, WO 2009139880, US 20110196150, WO 2008027542, WO 199854170, WO 199946258, and WO 2014018866, the contents of each of which is herein incorporated by reference.
• the IMiD-based IRAK degraders described and disclosed herein specifically degrade (a) an IRAK protein and (b) either (i) ikaros (IKZF1) (Ensembl Gene ID: ENG00000185811), (ii) aiolos (IKZF3) (Ensembl Gene ID: ENSG00000161405), or (iii) both ikaros and aiolos.
• IKZF1 Ensembl Gene ID: ENG00000185811
• aiolos IKZF3
• the present invention provides a compound of Formula I, wherein LBM is an IMiD-based cereblon E3 ubiquitin ligase binding moiety thereby forming a compound of formula I- oo-l, 1-00-2, 1-00-3, 1-00-4, 1-oo-5, 1-00-6, 1-oo-7, 1-00-8, 1-oo-9, or I-oo-lO respectively:
• I-oo-lO or a compound of formula I-oo'-l, I-oo'-2, 1-oo'-3, 1-oo'-4, 1-oo'-5, 1-oo'-6, 1-oo'-7, 1-oo'-8, 1-oo'-9, or I- oo'-lO respectively:
• the present invention provides a compound of formula I- oo-l, 1-00-2, 1-00-3, 1-00-4, 1-oo-5, 1-00-6, 1-oo-7, 1-00-8, 1-oo-9, 1-oo-lO, I-oo'-l, I-oo'-2, 1-oo'-3, 1-oo'- 4, 1-oo'-5, 1-oo'-6, I-oo'-7, 1-oo'-8, 1-oo'-9, 1-oo'-10, I-oo"-l, I-oo"-2, I-oo"-3, I-oo"-4, I-oo"-5, I-oo"- 6, I-oo"-7, I-oo"-8, I-oo"-9, or I-oo"-10, or a pharmaceutically acceptable salt thereof, wherein L and IRAK are as defined above and described in embodiments herein, and wherein:
• Y is a bond, Yi, O, NH, NR 2 , C(0)0, OC(O), C(0)NR 2 ', NR 2 'C(0), Yi— O, Yi— NH, Y i — NR 2 , Yi— C(O), Yi — C(0)0, Yi — OC(O), Yi — C(0)NR 2 ', or Yi — NR 2 'C(0), wherein Yi is Ci-Ce alkylene, G-G alkenylene, or G-G alkynylene;
• X is C(O) or C(R3)2;
• R2' is H, C I -G alkyl, G-G alkenyl, C 3 -C 8 cycloalkyl, or 3- to 8-membered heterocycloalkyl, and R2', when not being H, is optionally substituted with one or more of halogen, N(R a )2, NHC(0)R a , NHC(0)0R a , OR 3 ⁇ 4 , G-C 8 cycloalkyl, 3- to 8-membered heterocycloalkyl, G-Go aryl or 5- to 10- membered heteroaryl, wherein each of the G-G cycloalkyl, 3- to 8-membered heterocycloalkyl, G-Go aryl or 5- to 10-membered heteroaryl is optionally further substituted with one or more of halogen, N3 ⁇ 4, CN, nitro, OH, C(0)0H, G-G alkyl.
• R5 is H, G-G alkyl, F, or Cl; each R a independently is H or G-G alkyl;
• R b is H or tosyl; t is 0 or 1; m is 0, 1, 2 or 3; and n is 0, 1 or 2.
• the present invention provides a compound of Formula I, wherein LBM is an IMID-based cereblon E3 ubiquitin ligase binding moiety thereby forming a compound of formula I-oo- 1:
• R3 is hydrogen; two R 4 , together with the carbon atom to which they are attached, form C(O);
• R 5 is hydrogen or C 1 -C 3 alkyl; t is 1; m is 0, 1, 2, 3, or 4; and n is 0.
• the present invention provides a compound of Formula I, wherein LBM is an IMID-based cereblon E3 ubiquitin ligase binding moiety thereby forming a compound of formula I-oo- 1:
• R3 is hydrogen; two R 4 . together with the carbon atom to which they are attached, form C(O);
• R 5 is hydrogen or C 1 -C 3 alkyl; t is 1; m is 0, 1, 2 or 3; and n is 0.
• the present invention provides a compound of Formula I, wherein LBM is an IMID-based cereblon E3 ubiquitin ligase binding moiety thereby forming a compound of formula I- pp-1, 1-pp-2, 1-pp-3, 1-pp-4, 1-pp-5, or I-pp-6 respectively: or a pharmaceutically acceptable salt thereof, wherein L and IRAK are as defined above and described in embodiments herein, and wherein each of the variables A, G, G’, Qi, Q 2 , Q 3 , Q 4 , R, R’, W, X, Y, Z, ww , and n is as defined and described in WO 2016/197114 and US 2018/0147202, the entirety of each of which is herein incorporated by reference.
• the present invention provides a compound of formula I, wherein LBM is an IMID-based E3 ubiquitin ligase (cereblon) binding moiety thereby forming a compound of formula I-tt-1, 1-tt-2, 1-tt-3, 1-tt-4, 1-tt-5, 1-tt-6, 1-tt-7, or I-tt-8:
• I-tt-7 I-tt-8 or a pharmaceutically acceptable salt thereof wherein L and IRAK are as defined above and described in embodiments herein, and wherein each of the variables Ar, R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , A, L, x, y, and — is as described and defined in WO 2017/161119, the entirety of each of which is herein incorporated by reference.
• the present invention provides a compound of Formula I, wherein LBM is an IMID-based CRBN E3 ubiquitin ligase binding moiety thereby forming a compound of formula I-zz-1, 1-zz-2, 1-zz-3, 1-zz-4, or I-zz-5, respectively: or a pharmaceutically acceptable salt thereof, wherein L and IRAK are as defined above and described in embodiments herein, and wherein each of the variables A 1 , A 2 , A 3 , R 5 , G and Z is as defined and described in WO 2017/176958.
• the present invention provides a compound of Formula I, wherein LBM is an IMID-based CRBN E3 ubiquitin ligase binding moiety thereby forming a compound of formula I-zz'-l, I-zz"-l, I-zz'-2, 1-zz'-2, 1-zz'-3, 1-zz"-3, 1-zz'-4, 1-zz"-4, 1-zz'-5 or I-zz"-5 respectively: I-zz'-5 I-zz"-5 or a pharmaceutically acceptable salt thereof, wherein L and IRAK are as defined above and described in embodiments herein, and wherein each of the variables A 1 , A 2 , A 3 , R 5 , G and Z is as defined and described in WO 2017/176958, the entirety of which is herein incorporated by reference.
• the present invention provides a compound of Formula I, wherein LBM is an IMiD-based cereblon E3 ubiquitin ligase binding moiety, thereby forming a compound of formula I-ccc-1:
• I-ccc-1 or a pharmaceutically acceptable salt thereof, wherein L and IRAK is as defined above and described in embodiments herein, and wherein: each of X 1 , X 2 , and X 3 is independently a bivalent moiety selected from a covalent bond, -CH2-, -C(O)-,
• R 1 is hydrogen, deuterium, halogen, -CN, -OR, -SR,
• each of R 2 is independently hydrogen, R 6 , halogen, -CN, -NO2, -OR, -SR, -NR 2 , -S(0) 2 R, -S(0) 2 NR 2, -S(0)R, -C(0)R, -C(0)OR, -C(0)NR 2 ,
• each R 6 is independently an optionally substituted group selected from Ci- 6 aliphatic, phenyl, a 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur; each R is independently hydrogen, or an optionally substituted group selected from CYr, aliphatic, phenyl, a 4-7 membered saturated or partially unsaturated heterocyclic having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or: two R groups on the same
• Ring A is a fused ring selected from 6-membered aryl containing 0-2 nitrogen atoms, 5 to 7-membered partially saturated carbocyclyl, 5 to 7-membered partially saturated heterocyclyl with 1-2 heteroatoms independently selected from nitrogen, oxygen or sulfur, or 5-membered heteroaryl with 1-3 heteroatoms independently selected from nitrogen, oxygen or sulfur; and m is 0, 1, 2, 3 or 4.
• the present invention provides a compound of formula I-ccc-1, wherein LBM is an IMID-based cereblon E3 ubiquitin ligase (cereblon) binding moiety thereby forming a compound of formula I-ccc'-l or I-ccc"-l:
• I-ccc"-l or a pharmaceutically acceptable salt thereof, wherein IRAK, L, Ring A, X 1 , X 2 , X 3 , R 1 , R 2 and m are as described above.
• the present invention provides a compound of Formula I, wherein LBM is a IMID-based cereblon E3 ubiquitin ligase binding moiety thereby forming a compound of formula I-ccc-1:
• I-ccc-1 or a pharmaceutically acceptable salt thereof, wherein L and IRAK are as defined above and described in embodiments herein, and wherein:
• X 1 is -C(O)-
• X 2 is -C(O)-
• X 3 is -CH 2- or-C(O)-;
• R 1 is hydrogen or Ci-4 aliphatic; each of R 2 is independently hydrogen, halogen, Ci-4 aliphatic or -OCi- 4 aliphatic; Ring A is benzo; and m is 0, 1, 2, 3 or 4.
• each of X 1 , X 2 , and X 3 is independently a bivalent moiety selected from a covalent bond, -CH 2- , -C(O)-, -C(S)-, and
• X 1 is a covalent bond, -CH2-, -C(O)-, -C(S)-, or
• X 1 is selected from those depicted in Table 1, below.
• X 2 is a covalent bond, -CH2-, -C(O)-, -C(S)-, or [00119] In some embodiments, X 2 is selected from those depicted in Table 1, below.
• X 3 is a covalent bond, -CH2-, -C(O)-, -C(S)-, or .
• X 3 is selected from those depicted in Table 1, below.
• R 1 is hydrogen, deuterium, halogen, -CN, -OR, -SR,
• R 1 is hydrogen, deuterium, halogen, -CN, -OR, -SR, -S(0)R, -S(0) 2 R, -NR 2 , or an optionally substituted C 1-4 aliphatic.
• R 1 is selected from those depicted in Table 1, below.
• each of R 2 is independently hydrogen, R 6 , halogen, - CN, -NO2, -OR, -SR, -NR 2 , -S(0) 2 R, -S(0) 2 NR 2, -S(0)R, -C(0)R, -C(0)OR, -
• R 2 is hydrogen, R 6 , halogen, -CN, -NO2, -OR, -
• R 2 is selected from those depicted in Table 1, below.
• each R 6 is independently an optionally substituted group selected from Ci- 6 aliphatic, phenyl, a 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.
• R 6 is an optionally substituted Ci- 6 aliphatic group. In some embodiments, R 6 is an optionally substituted phenyl. In some embodiments, R 6 is an optionally substituted 4-7 membered saturated or partially unsaturated heterocyclic ring having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, R 6 is an optionally substituted 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur.
• R 6 is selected from those depicted in Table 1, below.
• Ring A is a fused ring selected from 6-membered aryl containing 0-2 nitrogen atoms, 5 to 7-membered partially saturated carbocyclyl, 5 to 7-membered partially saturated heterocyclyl with 1-2 heteroatoms independently selected from nitrogen, oxygen or sulfur, or 5- membered heteroaryl with 1-3 heteroatoms independently selected from nitrogen, oxygen or sulfur.
• Ring A is a fused 6-membered aryl containing 0-2 nitrogen atoms.
• Ring A is a fused 5 to 7-membered partially saturated carbocyclyl.
• Ring A is a fused 5 to 7-membered partially saturated heterocyclyl with 1-2 heteroatoms independently selected from nitrogen, oxygen or sulfur. In some embodiments Ring A is a fused 5- membered heteroaryl with 1-3 heteroatoms independently selected from nitrogen, oxygen or sulfur. [00133] In some embodiments, Ring A is a fused phenyl or benzo.
• Ring A is selected from those depicted in Table 1, below.
• m is 0, 1, 2, 3 or 4.
• m is 0. In some embodiments, m is 1. In some embodiments, m is 2. In some embodiments, m is 3. In some embodiments, m is 4.
• m is selected from those depicted in Table 1, below.
• each R is independently hydrogen, or an optionally substituted group selected from Ci-e aliphatic, phenyl, a 4-7 membered saturated or partially unsaturated heterocyclic having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur, and a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur, or: two R groups on the same nitrogen are optionally taken together with their intervening atoms to form a 4-7 membered saturated, partially unsaturated, or heteroaryl ring having 0-3 heteroatoms, in addition to the nitrogen, independently selected from nitrogen, oxygen, and sulfur.
• R is hydrogen. In some embodiments, R is phenyl. In some embodiments, R is a 4-7 membered saturated or partially unsaturated heterocyclic having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, R is a 5-6 membered heteroaryl ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, two R groups on the same nitrogen are optionally taken together with their intervening atoms to form a 4-7 membered saturated, partially unsaturated, or heteroaryl ring having 0-3 heteroatoms, in addition to the nitrogen, independently selected from nitrogen, oxygen, and sulfur.
• R is selected from those depicted in Table 1, below.
• LBM is In some embodiments, LBM is , some embodiments, , In some embodiments, some embodiments, some embodiments, some embodiments,
• LBM is selected from those in Table 1 below.
• the present invention provides a compound of formula I-a, wherein Ring Q is benzo and LBM is an IMID-based CRBN E3 ubiquitin ligase binding moiety , thereby providing a compound of formula I-a-6:
• the present invention provides a compound of formula I-a, wherein Ring Q is benzo and LBM is an IMID-based CRBN E3 ubiquitin ligase binding moiety , thereby providing a compound of formula I- a- 7:
• the present invention provides a compound of formula I-a, wherein
• Ring Q is benzo and LBM is CRBN E3 ubiquitin ligase binding moiety thereby providing a compound of formula I-a-8:
• the present invention provides a compound of formula I-a, wherein
• Ring Q is benzo and LBM is CRBN E3 ubiquitin ligase binding moiety thereby providing a compound of formula I-a-9:
• the present invention provides a compound of formula I-a, wherein thereby providing a compound of formula I-a- 10: a or a pharmaceutically acceptable salt thereof, wherein each of variables , Y, Ri, R 3 , R 3 ’, R 4 , Rs, t, m, n, Ring T, L, X, R x , R y , x, and y is as defined above and described in embodiments herein, both singly and in combination.
• the present invention provides a compound of formula I-a-2, wherein
• LBM is an IMID-based CRBN E3 ubiquitin ligase binding moiety thereby providing a compound of formula I-a-11:
• the present invention provides a compound of formula I-a-2, wherein LBM is CRBN E3 ubiquitin ligase binding moiety compound of formula I-a-12:
• the present invention provides a compound of formula I-a-2, wherein
• LBM is CRBN E3 ubiquitin ligase binding moiety compound of formula I-a-13:
• the present invention provides a compound of formula I-a-1, wherein thereby providing a compound of formula I-a-14:
• each of variables , Y, Ri, R 3 , R 3 ’, R 4 , Rs, t, m, n, L, L x , X, R x , R y , x, and y is as defined above and described in embodiments herein, both singly and in combination.
• the present invention provides a compound of formula I-a-1, wherein
• LBM is an IMID-based CRBN E3 ubiquitin ligase binding moiety thereby providing a compound of formula I-a-15:
• the present invention provides a compound of formula I-a-1, wherein
• LBM is CRBN E3 ubiquitin ligase binding moiety compound of formula I-a-16:
• the present invention provides a compound of formula I-a-1, wherein
• LBM is CRBN E3 ubiquitin ligase binding moiety compound of formula I-a-17:
• the present invention provides a compound of formula I-a-3, wherein thereby providing a compound of formula I-a-18: or a pharmaceutically acceptable salt thereof, wherein each of variables , Y, Ri, R 3 , R 3 ’, R 4 , Rs, t, m, n, Ring T, L, L x , R x , R y , x, and y is as defined above and described in embodiments herein, both singly and in combination.
• the present invention provides a compound of formula I-a-3, wherein
• LBM is an IMID-based CRBN E3 ubiquitin ligase binding moiety thereby providing a compound of formula I-a-19:
• the present invention provides a compound of formula I-a-3, wherein
• LBM is CRBN E3 ubiquitin ligase binding moiety compound of formula I-a-20:
• the present invention provides a compound of formula I-a-3, wherein LBM is CRBN E3 ubiquitin ligase binding moiety compound of formula I-a-21:
• the present invention provides a compound of formula I-a-4, wherein thereby providing a compound of formula I-a-22: t, m, n, L, L x , R x , R y , x, and y is as defined above and described in embodiments herein, both singly and in combination.
• the present invention provides a compound of formula I-a-4, wherein LBM is an IMID-based CRBN E3 ubiquitin ligase binding moiety thereby providing a compound of formula I-a-23:
• the present invention provides a compound of formula I-a-4, wherein
• LBM is CRBN E3 ubiquitin ligase binding moiety compound of formula I-a-24:
• the present invention provides a compound of formula I-a-4, wherein
• LBM is CRBN E3 ubiquitin ligase binding moiety compound of formula I-a-25:
• the present invention provides a compound of formula I-a-5, wherein thereby providing a compound of formula I-a-26: t, m, n, Ring T, L, R x , R y , x, and y is as defined above and described in embodiments herein, both singly and in combination.
• the present invention provides a compound of formula I-a-5, wherein
• LBM is an IMID-based CRBN E3 ubiquitin ligase binding moiety thereby providing a compound of formula I-a-27:
• the present invention provides a compound of formula I-a-5, wherein
• LBM is CRBN E3 ubiquitin ligase binding moiety compound of formula I-a-28:
• the present invention provides a compound of formula I-a-5, wherein
• LBM is CRBN E3 ubiquitin ligase binding moiety compound of formula I-a-29:
• the present invention provides a compound of formula I-a, wherein
• Ring Q is benzo, L is , and LBM is an IMID-based CRBN E3 ubiquitin ligase or a pharmaceutically acceptable salt thereof, wherein each of variables , Y, Ri, R 3 , R 3 ’, R 4 , Rs, t, m, n, Ring T, L x , X, R x , R y , x, and y is as defined above and described in embodiments herein, both singly and in combination.
• the present invention provides a compound of formula I-a, wherein
• Ring Q is benzo, L is , and LBM is an IMID-based CRBN E3 ubiquitin ligase binding moiety , thereby providing a compound of formula I-a-31: or a pharmaceutically acceptable salt thereof, wherein each of variables X 1 , X 2 , X 3 , R 1 , R 2 , Ring A, m, Ring T, L x , X, R x , R y , x, and y is as defined above and described in embodiments herein, both singly and in combination.
• L is a bivalent moiety that connects IRAK to LBM.
• L is a bivalent moiety that connects IRAK to LBM.
• L is a covalent bond or a bivalent, saturated or unsaturated, straight or branched Ci-50 hydrocarbon chain, wherein 0-6 methylene units of L are independently replaced by - C(D)(H)-, -C(D) 2 -, -CRF-, -CF2-, -Cy-, -0-, -N(R)-, -SI(R) 2 -, -Si(OH)(R)-, -SI(OH) 2 -, -P(0)(OR)-, - P(0)(R)-, -P(0)(NR 2 )-, -S-, -OC(O)-, -C(0)0-, -C(O)-, -S(O)-, -S(0) 2 -, -N(R)S(0) 2 -, -S(0) 2 N(R)-, - independently an optionally substituted bivalent ring selected from phenylenyl,
• each -Cy- is independently an optionally substituted bivalent phenylenyl. In some embodiments, each -Cy- is independently an optionally substituted 8-10 membered bicyclic arylenyl. In some embodiments, each -Cy- is independently an optionally substituted 4-7 membered saturated or partially unsaturated carbocyclylenyl. In some embodiments, each -Cy- is independently an optionally substituted 4-12 membered saturated or partially unsaturated spiro carbocyclylenyl. In some embodiments, each -Cy- is independently an optionally substituted 8-10 membered bicyclic saturated or partially unsaturated carbocyclylenyl.
• each -Cy- is independently an optionally substituted 4-7 membered saturated or partially unsaturated heterocyclylenyl having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, each -Cy- is independently an optionally substituted 4-12 membered saturated or partially unsaturated spiro heterocyclylenyl having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, each -Cy- is independently an optionally substituted 8-10 membered bicyclic saturated or partially unsaturated heterocyclylenyl having 1-2 heteroatoms independently selected from nitrogen, oxygen, and sulfur.
• each -Cy- is independently an optionally substituted 5-6 membered heteroarylenyl having 1-4 heteroatoms independently selected from nitrogen, oxygen, and sulfur. In some embodiments, each -Cy- is independently an optionally substituted 8-10 membered bicyclic heteroarylenyl having 1-5 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
• -Cy In some embodiments, -Cy- is , y In some embodiments, -Cy- is H . In some embodiments, -Cy- is H H In some embodiments, -Cy- is
• -Cy- is ⁇ y In some embodiments, -Cy- is me embodiments, -Cy- is In some embodiments, -Cy- is me embodiments, -Cy- is ⁇ OO-» In some embodiments, -Cy- is . In some embodiments,
• -Cy- is selected from those depicted in Table 1, below.
• L is selected from those depicted in Table 1, below.
• r is 0. In some embodiments, r is 1. In some embodiments, r is 2. In some embodiments, r is 3. In some embodiments, r is 4. In some embodiments, r is 5. In some embodiments, r is 6. In some embodiments, r is 7. In some embodiments, r is 8. In some embodiments, r is 9. In some embodiments, r is 10.
• r is selected from those depicted in Table 1, below.
• L is iz .
• L N . In some embodiments, L is . In some embodiments, L is In some embodiments, L is some embodiments, L is O In some embodiments, L is . , . In some embodiments, In some embodiments, L is . , , , . , . In , , , , , some embodiments, L is In some embodiments, L is ,
• L is In some embodiments, L is ,
• L is . In some embodiments, L is some embodiments, L is O . In some embodiments, L is , , embodiments, L is O In some embodiments, L is . , In some ,
• L is O O
• L is In some embodiments, L is , , , , embodiments, L is O In some embodiments, L is . , . In some embodiments, , , In some , , some embodiments, L is In some embodiments, L is , , , In some embodiments, L is In some embodiments, L is some embodiment, L is O In some embodiment, L is , some embodiments, L is In some embodiments, L is , . , . In some embodiments, L is O In some embodiments, L is In some embodiments, L is In some embodiments, L is .
• L is In some embodiments, L is H In some embodiments, L some embodiments, L In some embodiments, In In some embodiments, L is , In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is , , In some embodiments, L is In some embodiments, L is ,
• L is j n some embodiments, L is n some em o mens, s . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is
• L is In some embodiments, L is In In some embodiments, L is , some embodiments, L is In some embodiments, L is , In some embodiments, In some embodiments, L is , . In some embodiments, L is In some embodiments, L is some embodiments, L is some embodiments, L is O . In some embodiments, L is ,
• L is In some embodiment, L is , n some em o mens, IS ,
• L is In some embodiments, L is , some embodiments, L is In some embodiments, L is . , . In some embodiments, L is In some embodiments, L is , In some embodiments, L . In some embodiments, L ,is
• L is In some embodiments, L is . , In some em o mens, s some embodiments, L is O In some embodiments, L is , . In some embodiments, L is In some embodiments, L is , In some embodiments, L is , In some embodiments, L is ,
• L is In some embodiments, L is In some embodiments, L is In some embodiments, L is . In some embodiments, L is . , In some embodiments, L is In some embodiments, L is , some embodiments, L is . In some embodiments, L is , , . , . In some embodiments, some embodiments, , , embodiments, L is HO OH . In some embodiments, L is . , In some
• L is . In some embodiments, L is . In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is , In some embodiments, , In some , s ,
• L is In some embodiments, L is ,
• L is In some embodiments, L is
• L is O . In some embodiments, L is In some embodiments, L is ,
• L is In some embodiments, L is ,
• L is . In some embodiments, L is , In some embodiments, L is In some embodiments, L is , some embodiments, L is In some embodiments, L is ,
• L is In some embodiments, L is , In some embodiments, L is In some embodiments, L is some embodiments, L is In some embodiments, L is , In some embodiments, L is . In some embodiments,
• L is In some embodiments, L is , . I ,n some embodiments, L is In some embodiments, L is
• L is In some embodiments, L is . , . In some embodiments, L is , some
• H H embodi , .
• L is , , some embodiments, L is In some embodiments, L , In , , In some embodiments, L is In some embodiments, L is TIn some embodiments, L is In some embodiments, L is , In some embodiments, L is . In some embodiments, ,
• L is In some embodiments, L is , In some embodiments, L is In some embodiments, L is In some embodiments, L is , some embodiments, L is In some embodiments, L is , . In some embodiments, , In some embodiments, L is In some embodiments, L is , In some embodiments, L is In some embodiments, L is , In some embodiments
• L is .
• L is In some embodiments, L is , In some embodiments, , In some embodiments, L is O . In some embodiments, L is In some embodiments, L is In some
• L is In some embodiments, L is . In some embodiments, L is In some embodiments, L is In some embodiments, L is , embodiments, L is . In some embodiments,
• L is In some embodiments, L is , embodiments, L is In some embodiments, L is a covalent bond. In some embodiments, L is A . In some embodiments, L is In some embodiments, L is , In some embodiments, L is . , In some embodiments, L is , In some embodiments, L is . In some embodiments, L is a covalent bond. In some embodiments, L is In some embodiments, L is , . In some embodiments, L is In some embodiments, L is In some embodiments, L is H In some embodiments, L is , In some embodiments, L is . In some embodiments, L is . In some embodiments, L is
• L is In some embodiments, L is . , n some embodiments,
• L is . In some embodiments, L is . In some embodiments, L is In some embodiments, L is . , In some
• L is . In some embodiments, L is
• L is . In some embodiments, L is embodiments, L is H . In some embodiments, L is / ' - / . In some embodiments, L is In some embodiments, L is In some embodiments, L is . , . In some embodiments, L is In some embodiments, L is In some embodiments, L . In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is , In some embodiments, L is , . In some embodiments, L is In some embodiments, L is . In some embodiments, L is some embodiments, L is . In some embodiments, L is some embodiments, L is some embodiments, L is some embodiments, L is some embodiments, L is some embodiments, L is some embodiments, L is some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiment
• L is In some embodiments, L is , In some embodiments, L is In some embodiments, L is , In some embodiments, L is . In some embodiments, L is In some embodiments, L is In some embodiments, L is . In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is In
• L is . In some embodiments, L is . In some embodiments, L is In In some embodiments, L is , . In some embodiments,
• L is In some embodiments, L is , In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is some embodiments, L is H . In some embodiments, L is , . In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is , some embodiments, L is In some embodiments, L is . In In some embodiments, L is In some In In some embodiments, L is , . In some embodiments, L is . , . In some embodiments, L is In some embodiments, L is In some embodiments, L is , i n some embodiments, L is In some embodiments, L is , .
• L is In some embodiments, L is , In some embodiments, L is . In some embodiments, L is . In some embodiments, L is some embodiments, L is , In some embodiments, L is In some embodiments, L is , In some embodiments, L is , In some embodiments, L is , In some embodiments, L is , In some embodiments, L is , In some embodiments, L is , In some embodiments, L is , In
• L is . In some embodiments, L is .
• L is H In some embodiments, L is . ,
• L is In some embodiments, L is , In some
• L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is
• L is In some embodiments, L is embodiments, L is O In some embodiments, L is , In some embodiments, L is . In some embodiments, L i ios
• L is In some embodiments, L is , In some embodiments, L is In some embodiments, L is , . In some embodiments, L is In some embodiments, L is . , . In some embodiments, L is In some embodiments, L is , L is ,
• L is In some embodiments, L is i . , . ,
• L is In some embodiments, L is In some
• L is . In some embodiments, L is
• L is In some embodiments, L is , In some embodiments, L is O . In some embodiments, L is . In some U ⁇ 'N' ⁇ C O / '' ' -' ⁇ embodiments, L is In some embodiments, L is , In some embodiments, L is In some embodiments, L is In some embodiments, L is . , . In some embodiments, In some embodiments, L is . , . In some
• L is In some embodiments, L is In some e , , some embodiments, L is In some embodiments, L is , In some embodiments, some H embodiments, L is . In some embodiments, L is
• L is . In some embodiments, L is
• L is In some embodiments, L is , In some embodiments, L is . In some embodiments, L is . In some embodiments, L is
• L is In some embodiments, L is . , . In some embodiments, L , . In some embodiments, L is In some embodiments, L is , In some embodiments, L is i n some embodiments, L is . In some embodiments, L n some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments,
• L is In some embodiments, L is In some embodiments, L is . , . In some embodiments, L is , ,
• L is In some embodiments, L is , i odiments, L is / H emb — N y f . In some embodiments, L is . In some embodiments, L
• L is In some embodiments, L is . In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is
• L is . , . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is
• L is In some embodiments, L is , In some emb worked L is v — I some emb Struktur L is . , . In some embodiments, L is In some embodiments, L is In some embodiments, L is , In some embodiments, L is In some embodiments, L is , . In O some embodiments, L is In some embodiments, L is , In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is In some embodiments, L is , . In some embodiments, L is c ' In some embodiments, L is
• L is . In some embodiments, L is In some embodiments, L is In some embodiments, L is . In some embodiments, L is . In some embodiments, L is In some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is . In some embodiments, L is In some embodiments, L is .
• L is A ' O- . In some embodiments, L is , In some embodiments, L , -
• L is selected from those depicted in Table 1, below.
• the point of attachment of L to IRAK and LBM can be, for example when
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein IRAK is HO ' , LBM is selected from any of those in Table A below, and L is selected from any of those in Table B below. [00200] In some embodiments, a provided compound or pharmaceutically acceptable salt thereof, is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• a provided compound or pharmaceutically acceptable salt thereof is selected from those wherein I selected from any of those in Table A below, and L is selected from any of those in Table B below.
• the present invention provides a compound having an IRAK binding moiety described and disclosed herein, a LBM set forth in Table A above, and a linker set forth in Table B above, or a pharmaceutically acceptable salt thereof.
• the present invention provides a compound as depicted in Table 1, above, or a pharmaceutically acceptable salt thereof.
• the present invention provides a compound of formula I, wherein the compound is not any of the compounds depicted in Table 1A, below.
• the present invention does not provide a compound set forth in Table 1A, above, or a pharmaceutically acceptable salt thereof. 4. General Methods of Providing the Present Compounds
• the compounds of this invention may be prepared or isolated in general by synthetic and/or semi-synthetic methods known to those skilled in the art for analogous compounds and by methods described in detail in the Examples, herein.
• oxygen protecting group includes, for example, carbonyl protecting groups, hydroxyl protecting groups, etc.
• Hydroxyl protecting groups are well known in the art and include those described in detail in Protecting Groups in Organic Synthesis, T. W. Greene and R G. M. Wuts, 3 rd edition, John Wiley & Sons, 1999, the entirety of each of which is herein incorporated by reference.
• suitable hydroxyl protecting groups include, but are not limited to, esters, allyl ethers, ethers, silyl ethers, alkyl ethers, arylalkyl ethers, and alkoxyalkyl ethers.
• esters include formates, acetates, carbonates, and sulfonates.
• Specific examples include formate, benzoyl formate, chloroacetate, trifluoroacetate, methoxyacetate, triphenylmethoxyacetate, p-chlorophenoxyacetate, 3- phenylpropionate, 4-oxopentanoate, 4,4-(ethylenedithio)pentanoate, pivaloate (trimethylacetyl), crotonate, 4-methoxy-crotonate, benzoate, p-benylbenzoate, 2,4,6-trimethylbenzoate, carbonates such as methyl, 9- fluorenylmethyl, ethyl, 2,2,2-trichloroethyl, 2-(trimethylsilyl)ethyl, 2-(phenylsulfonyl)ethyl, vinyl, allyl, and p-nitrobenzyl.
• silyl ethers examples include trimethylsilyl, triethyl silyl, t-butyldimethylsilyl, t-butyldiphenylsilyl, triisopropylsilyl, and other trialkylsilyl ethers.
• Alkyl ethers include methyl, benzyl, p- methoxybenzyl, 3,4-dimethoxybenzyl, trityl, t-butyl, allyl, and allyloxycarbonyl ethers or derivatives.
• Alkoxyalkyl ethers include acetals such as methoxymethyl, methylthiomethyl, (2-methoxyethoxy)methyl, benzyloxymethyl, beta-(trimethylsilyl)ethoxymethyl, and tetrahydropyranyl ethers.
• arylalkyl ethers include benzyl, p-methoxybenzyl (MPM), 3,4-dimethoxybenzyl, O-nitrobenzyl, p-nitrobenzyl, p-halobenzyl, 2,6-dichlorobenzyl, p-cyanobenzyl, and 2- and 4-picolyl.
• Amino protecting groups are well known in the art and include those described in detail in Protecting Groups in Organic Synthesis, T. W. Greene and R G. M. Wuts, 3 rd edition, John Wiley & Sons, 1999, the entirety of each of which is herein incorporated by reference.
• Suitable amino protecting groups include, but are not limited to, aralkylamines, carbamates, cyclic imides, allyl amines, amides, and the like.
• Examples of such groups include t-butyloxycarbonyl (BOC), ethyloxycarbonyl, methyloxycarbonyl, trichloroethyloxycarbonyl, allyloxycarbonyl (Alloc), benzyloxocarbonyl (CBZ), allyl, phthalimide, benzyl (Bn), fluorenylmethylcarbonyl (Fmoc), formyl, acetyl, chloroacetyl, dichloroacetyl, trichloroacetyl, phenylacetyl, trifluoroacetyl, benzoyl, and the like.
• a provided compound is formed having a reactive DIM moiety (e.g., amine, alcohol, etc.), it is not shown but it is generally appreciated and well known by those having ordinary skill in the art that the reactivity of said reactive DIM moiety may be masked by employing a suitable protecting group that can thereafter be removed in situ or during a separate synthetic step.
• a reactive DIM moiety e.g., amine, alcohol, etc.
• amine A-l is coupled to acid A-2 using the coupling agent HATU in the presence of the base DIPEA in DMF to form a compound of the invention with a linker comprising an amide bond.
• the squiggly bond, ' /vww ' represents the portion of the linker between IRAK and the terminal amino group of A-l or the portion of the linker between DIM and the terminal carboxyl group of A-2, respectively.
• an amide bond can be formed using coupling reagents known in the art such as, but not limited to DCC, DIC, EDC, HBTU, HCTU, PyAOP, PyBrOP, BOP, BOP-C1, DEPBT, T3P, TATU, TBTU, TNTU, TOTU, TPTU, TSTU, or TDBTU.
• coupling reagents known in the art such as, but not limited to DCC, DIC, EDC, HBTU, HCTU, PyAOP, PyBrOP, BOP, BOP-C1, DEPBT, T3P, TATU, TBTU, TNTU, TOTU, TPTU, TSTU, or TDBTU.
• amine A-l is coupled to acid A-2 using the coupling agent PyBOP in the presence of the base DIPEA in DMF to form a compound of the invention with a linker comprising an amide bond.
• the squiggly bond, ' /vww ' represents the portion of the linker between IRAK and the terminal amino group of A-l or the portion of the linker between DIM and the terminal carboxyl group of A-2, respectively.
• an amide bond can be formed using coupling reagents known in the art such as, but not limited to DCC, DIC, EDC, HBTU, HCTU, PyAOP, PyBrOP, BOP, BOP-C1, DEPBT, T3P, TATU, TBTU, TNTU, TOTU, TPTU, TSTU, or TDBTU.
• coupling reagents known in the art such as, but not limited to DCC, DIC, EDC, HBTU, HCTU, PyAOP, PyBrOP, BOP, BOP-C1, DEPBT, T3P, TATU, TBTU, TNTU, TOTU, TPTU, TSTU, or TDBTU.
• acid A-3 is coupled to amine A-4 using the coupling agent HATU in the presence of the base DIPEA in DMF to form a compound of the invention with a linker comprising an amide bond.
• the squiggly bond represents the portion of the linker between IRAK and the terminal carboxyl group of A-3 or the portion of the linker between DIM and the terminal amino group of A-4, respectively.
• an amide bond can be formed using coupling reagents known in the art such as, but not limited to DCC, DIC, EDC, HBTU, HCTU, PyAOP, PyBrOP, BOP, BOP-C1, DEPBT, T3P, TATU, TBTU, TNTU, TOTU, TPTU, TSTU, or TDBTU.
• coupling reagents known in the art such as, but not limited to DCC, DIC, EDC, HBTU, HCTU, PyAOP, PyBrOP, BOP, BOP-C1, DEPBT, T3P, TATU, TBTU, TNTU, TOTU, TPTU, TSTU, or TDBTU.
• acid A-3 is coupled to amine A-4 using the coupling agent PyBOP in the presence of the base DIPEA in DMF to form a compound of the invention with a linker comprising an amide bond.
• the squiggly bond, ' /vww ' represents the portion of the linker between IRAK and the terminal carboxyl group of A-3 or the portion of the linker between DIM and the terminal amino group of A-4, respectively.
• an amide bond can be formed using coupling reagents known in the art such as, but not limited to DCC, DIC, EDC, HBTU, HCTU, PyAOP, PyBrOP, BOP, BOP-C1, DEPBT, T3P, TATU, TBTU, TNTU, TOTU, TPTU, TSTU, or TDBTU.
• coupling reagents known in the art such as, but not limited to DCC, DIC, EDC, HBTU, HCTU, PyAOP, PyBrOP, BOP, BOP-C1, DEPBT, T3P, TATU, TBTU, TNTU, TOTU, TPTU, TSTU, or TDBTU.
• an S N AC displacement of fluoride A-6 by amine A-5 is effected in the presence of the base DIPEA in DMF to form a compound of the invention with a linker comprising a secondary amine.
• the squiggly bond, /vww ' represents the portion of the linker between IRAK and the terminal amino group of A-5.
• an S N AT displacement of fluoride A-7 by amine A-8 is effected in the presence of the base DIPEA in DMF to form a compound of the invention with a linker comprising a secondary amine.
• the squiggly bond, - LLLLL/ . represents the portion of the linker between DIM and the terminal amino group of A-8.
• reductive alkylation of aldehyde A-9 by amine A-10 is effected in the presence of a mild hydride source (e.g., sodium cyanoborohydride or sodium triacetoxyborohydride) to form a provided compound with a linker comprising a secondary amine.
• a mild hydride source e.g., sodium cyanoborohydride or sodium triacetoxyborohydride
• the squiggly bond, ' LLLLL represents the portion of the linker between DIM and the terminal amino group of
• reductive alkylation of aldehyde A-12 by amine A-ll is effected in the presence of a mild hydride source (e.g., sodium cyanoborohydride or sodium triacetoxyborohydride) to form a provided compound with a linker comprising a secondary amine.
• a mild hydride source e.g., sodium cyanoborohydride or sodium triacetoxyborohydride
• Jmiw represents the portion of the linker between IRAK and the terminal amino group of A-ll.
• compositions are provided.
• the invention provides a composition comprising a compound of this invention or a pharmaceutically acceptable derivative thereof and a pharmaceutically acceptable carrier, adjuvant, or vehicle.
• the amount of compound in compositions of this invention is such that it is effective to measurably degrade and/or inhibit an IRAK protein kinase, or a mutant thereof, in a biological sample or in a patient.
• the amount of compound in compositions of this invention is such that it is effective to measurably degrade and/or inhibit an IRAK protein kinase, or a mutant thereof, in a biological sample or in a patient.
• a composition of this invention is formulated for administration to a patient in need of such composition.
• a composition of this invention is formulated for oral administration to a patient.
• patient means an animal, preferably a mammal, and most preferably a human.
• compositions of this invention refers to a non-toxic carrier, adjuvant, or vehicle that does not destroy the pharmacological activity of the compound with which it is formulated.
• Pharmaceutically acceptable carriers, adjuvants or vehicles that may be used in the compositions of this invention include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene- polyoxyprop
• a “pharmaceutically acceptable derivative” means any non-toxic salt, ester, salt of an ester or other derivative of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily or degratorily active metabolite or residue thereof.
• the term "inhibitorily active metabolite or residue thereof' means that a metabolite or residue thereof is also an inhibitor of an IRAK protein kinase, or a mutant thereof.
• the term "degratorily active metabolite or residue thereof means that a metabolite or residue thereof is also a degrader of an IRAK protein kinase, or a mutant thereof.
• compositions of the present invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir.
• parenteral as used herein includes subcutaneous, intravenous, intramuscular, intra-articular, intra-synovial, intrastemal, intrathecal, intrahepatic, intralesional and intracranial injection or infusion techniques.
• the compositions are administered orally, intraperitoneally or intravenously.
• Sterile injectable forms of the compositions of this invention may be aqueous or oleaginous suspension. These suspensions may be formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents.
• the sterile injectable preparation may also be a sterile injectable solution or suspension in a non -toxic parenterally acceptable diluent or solvent, for example as a solution in 1,3-butanediol.
• a non -toxic parenterally acceptable diluent or solvent for example as a solution in 1,3-butanediol.
• acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution.
• sterile, fixed oils are conventionally employed as a solvent or suspending medium.
• any bland fixed oil may be employed including synthetic mono- or di glycerides.
• Fatty acids such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically-acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions.
• These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, such as carboxymethyl cellulose or similar dispersing agents that are commonly used in the formulation of pharmaceutically acceptable dosage forms including emulsions and suspensions.
• Other commonly used surfactants such as Tweens, Spans and other emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms may also be used for the purposes of formulation.
• compositions of this invention may be orally administered in any orally acceptable dosage form including, but not limited to, capsules, tablets, aqueous suspensions or solutions.
• carriers commonly used include lactose and com starch.
• Lubricating agents such as magnesium stearate, are also typically added.
• useful diluents include lactose and dried cornstarch.
• aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening, flavoring or coloring agents may also be added.
• compositions of this invention may be administered in the form of suppositories for rectal administration.
• suppositories for rectal administration.
• suppositories can be prepared by mixing the agent with a suitable non-irritating excipient that is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug.
• suitable non-irritating excipient include cocoa butter, beeswax and polyethylene glycols.
• compositions of this invention may also be administered topically, especially when the target of treatment includes areas or organs readily accessible by topical application, including diseases of the eye, the skin, or the lower intestinal tract. Suitable topical formulations are readily prepared for each of these areas or organs.
• Topical application for the lower intestinal tract can be effected in a rectal suppository formulation (see above) or in a suitable enema formulation. Topically-transdermal patches may also be used.
• compositions may be formulated in a suitable ointment containing the active component suspended or dissolved in one or more carriers.
• Carriers for topical administration of compounds of this invention include, but are not limited to, mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene compound, emulsifying wax and water.
• provided pharmaceutically acceptable compositions can be formulated in a suitable lotion or cream containing the active components suspended or dissolved in one or more pharmaceutically acceptable carriers.
• Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.
• compositions may be formulated as micronized suspensions in isotonic, pH adjusted sterile saline, or, preferably, as solutions in isotonic, pH adjusted sterile saline, either with or without a preservative such as benzylalkonium chloride.
• the pharmaceutically acceptable compositions may be formulated in an ointment such as petrolatum.
• compositions of this invention may also be administered by nasal aerosol or inhalation.
• Such compositions are prepared according to techniques well-known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other conventional solubilizing or dispersing agents.
• compositions of this invention are formulated for oral administration. Such formulations may be administered with or without food. In some embodiments, pharmaceutically acceptable compositions of this invention are administered without food. In other embodiments, pharmaceutically acceptable compositions of this invention are administered with food. [00250]
• the amount of compounds of the present invention that may be combined with the carrier materials to produce a composition in a single dosage form will vary depending upon the host treated, the particular mode of administration. Preferably, provided compositions should be formulated so that a dosage of between 0.01 - 100 mg/kg body weight/day of the compound can be administered to a patient receiving these compositions.
• a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated.
• the amount of a compound of the present invention in the composition will also depend upon the particular compound in the composition.
• Compounds and compositions described herein are generally useful for the degradation and/or inhibition of kinase activity of one or more enzymes.
• Examples of kinases that are degraded and/or inhibited by the compounds and compositions described herein and against which the methods described herein are useful include those of the interleukin- 1 receptor-associated kinase (IRAK) family of kinases, the members of which include IRAK-1, IRAK-2, and IRAK-4, or a mutant thereof.
• IRAK interleukin- 1 receptor-associated kinase
• the activity of a compound utilized in this invention as a degrader and/or inhibitor of IRAK- 1, IRAK-2, and/or IRAK-4, or a mutant thereof may be assayed in vitro, in vivo or in a cell line.
• In vitro assays include assays that determine inhibition of either the phosphorylation activity and/or the subsequent functional consequences, or ATPase activity of activated IRAK-1, IRAK-2, and/or IRAK-4, or a mutant thereof. Alternate in vitro assays quantitate the ability of the inhibitor to bind to IRAK-1, IRAK-2 and/or IRAK-4.
• Inhibitor binding may be measured by radiolabeling the inhibitor prior to binding, isolating the inhibitor/IRAK-1, inhibitor/IRAK-2, or inhibitor/IRAK-4 complex and determining the amount of radiolabel bound.
• inhibitor binding may be determined by running a competition experiment where new inhibitors are incubated with IRAK-1, IRAK-2, and/or IRAK-4 bound to known radioligands.
• Representative in vitro and in vivo assays useful in assaying an IRAK-4 inhibitor include those described and disclosed in, e.g., Kim et al., “A critical role for IRAK4 kinase activity in Toll-like receptor-mediated innate immunity,” J. Exp. Med.
• IRAK-4 The best characterized member of the IRAK family is the serine/threonine kinase IRAK-4.
• IRAK-4 is implicated in signaling innate immune responses from Toll-like receptors (TLRs) and Toll/IL-1 receptors (TIRs).
• TLRs Toll-like receptors
• TIRs Toll/IL-1 receptors
• Innate immunity detects pathogens through the recognition of pathogen-associated molecular patterns by TLRs, when then links to the adaptive immune response.
• TLRs recognize conserved structures of both microbes and endogenous molecules.
• TLRs which recognize bacterial and fungal components are located on the cell surface, whereas TLRs which recognize viral or microbial nucleic acids are localized to intracellular membranes such as endosomes and phagosomes.
• Cell surface TLRs can be targeted by small molecules and antibodies, whereas intracellular TLRs require targeting with oligonucleotides.
• TLRs mediate the innate immune response by upregulating the expression of inflammatory genes in multiple target cells. See, e.g., Sen et al., “Transcriptional signaling by double-stranded RNA: role ofTLR3,” Cytokine & Growth Factor Rev. 2005, 16, 1-14, incorporated by reference in its entirety. While TLR-mediated inflammatory response is critical for innate immunity and host defense against infections, uncontrolled inflammation is detrimental to the host leading to sepsis and chronic inflammatory diseases, such as chronic arthritis, atherosclerosis, multiple sclerosis, cancers, autoimmune disorders such as rheumatoid arthritis, lupus, asthma, psoriasis, and inflammatory bowel diseases.
• NF-KB nuclear factor-kB
• MAP mitogen-activated protein
• IL-8 interferon-regulatory factor cascades
• IRAK-4 The kinase activity of IRAK-4 has been shown to play a critical role in the TLR-mediated immune and inflammatory responses.
• IRAK4 is a key mediator of the innate immune response orchestrated by interleukin-1 receptor (IL-1R), interleukin- 18 receptor (IL-18R), IL-33 receptor (IL-33R), and Toll-like receptors (TLRs).
• IL-1R interleukin-1 receptor
• IL-18R interleukin- 18 receptor
• IL-33R IL-33 receptor
• TLRs Toll-like receptors
• IRAKI A critical signaling mediator of innate immunity
• Cellular Signaling 2008 20, 269-276
• Kim et ah “A critical role for IRAK4 kinase activity in Toll-like receptor-mediated innate immunity” J. Exp. Med. 2007 204(5), 1025-1036
• Koziczak-Holbro et ah “IRAK-4 Kinase Activity Is Required for Interleukin- 1 (IL-1) Receptor- and Toll-like Receptor 7-mediated Signaling and Gene Expression,” J. Biol. Chem.
• Lin et ak “Helical assembly in the MyD88-IRAK4-IRAK2 complex in TLR /IL-1R signalling,” Nature 2010, 465(17), 885- 891; Suzuki et ak, “IRAK-4 as the central TIR signaling mediator in innate immunity,” TRENDS in Immunol. 2002, 23(10), 503-506; Suzuki et ak, “Severe impairment of interleukin- 1 and Toll-like receptor signalling in mice lacking IRAK-4,” Nature 2002, 416, 750-754; Swantek et ak, “IL-1 Receptor-Associated Kinase Modulates Host Responsiveness to Endotoxin,” J. Immunol.
• mice are resistant to joint and bone inflammation/destruction in an arthritis model, suggesting that IRAK-4 may be targeted to treat chronic inflammation.
• IRAK-4 appears to be vital for childhood immunity against some pyogenic bacteria, it has been shown to play a redundant role in protective immunity to most infections in adults, as demonstrated by one study in which patients older than 14 lacking IRAK-4 activity exhibited no invasive infections. Cohen et ah, “Targeting protein kinases for the development of anti-inflammatory drugs,” Curr. Opin. Cell Bio.
• Representative IRAK-4 inhibitors include those described and disclosed in e.g., Buckley et ah, Bioorg. Med. Chem. Lett. 2008, 18, 3211-3214; Buckley et ah, Bioorg. Med. Chem. Lett. 2008, 18, 3291- 3295; Buckley et ah, Bioorg. Med. Chem. Lett. 2008, 18, 3656-3660; Powers et ah, “Discovery and initial SAR of inhibitors of interleukin- 1 receptor-associated kinase-4,” Bioorg. Med. Chem. Lett. 2006, 16, 2842- 2845; Wng et ah, “IRAK-4 Inhibitors for Inflammation,” Curr. Topics in Med. Chem. 2009, 9, 724-737, each of, the entirety of each of which is herein incorporated by reference.
• treatment refers to reversing, alleviating, delaying the onset of, or inhibiting the progress of a disease or disorder, or one or more symptoms thereof, as described herein.
• treatment may be administered after one or more symptoms have developed.
• treatment may be administered in the absence of symptoms.
• treatment may be administered to a susceptible individual prior to the onset of symptoms (e.g., in light of a history of symptoms and/or in light of genetic or other susceptibility factors). Treatment may also be continued after symptoms have resolved, for example to prevent or delay their recurrence.
• the present invention provides a method for treating a IRAK- 1 -mediated, a IRAK-2 -mediated, and/or a IRAK-4-mediated disorder comprising the step of administering to a patient in need thereof a compound of the present invention, or pharmaceutically acceptable composition thereof.
• IRAK- 1 -mediated means any disease or other deleterious condition in which one or more of IRAK-1, IRAK-2, and/or IRAK-4, or a mutant thereof, are known to play a role.
• another embodiment of the present invention relates to treating or lessening the severity of one or more diseases in which one or more of IRAK-1, IRAK-2, and/or IRAK-4, or a mutant thereof, are known to play a role.
• the present invention provides a method for treating one or more disorders, diseases, and/or conditions wherein the disorder, disease, or condition is a cancer, a neurodegenative disorder, a viral disease, an autoimmune disease, an inflammatory disorder, a hereditary disorder, a hormone-related disease, a metabolic disorder, conditions associated with organ transplantation, immunodeficiency disorders, a destructive bone disorder, a proliferative disorder, an infectious disease, a condition associated with cell death, thrombin-induced platelet aggregation, liver disease, pathologic immune conditions involving T cell activation, a cardiovascular disorder, or a CNS disorder.
• the disorder, disease, or condition is a cancer, a neurodegenative disorder, a viral disease, an autoimmune disease, an inflammatory disorder, a hereditary disorder, a hormone-related disease, a metabolic disorder, conditions associated with organ transplantation, immunodeficiency disorders, a destructive bone disorder, a proliferative disorder, an infectious disease, a condition associated with cell death, throm
• Diseases and conditions treatable according to the methods of this invention include, but are not limited to, cancer (see, e.g.. Ngo, V. et ah, “Oncogenically active MYD88 mutations in human lymphoma,” Nature, vol. 000, pp: 1-7 (2010); Lust, J.
• et ah “Hyperalgesia, synovitis and multiple biomarkers of inflammation are suppressed by interleukin 1 inhibition in a novel animal model of gouty arthritis,” Annals of Rheumatic Diseases, 68, pp: 1602-1608 (2009)), neurological disorders, metabolic syndrome (see, e.g., Troseid, M. “The role of interleukin- 18 in the metabolic syndrome,” Cardiovascular Diahetology, 9: 11, pp: 1-8 (2010)), immunodeficiency disorders such as AIDS and HIV (see, e.g., Iannello, A.
• a human patient is treated with a compound of the current invention and a pharmaceutically acceptable carrier, adjuvant, or vehicle, wherein said compound is present in an amount to measurably degrade and/or inhibit IRAK-1 only, IRAK-2-only, IRAK-4-only and/or IRAKI and IRAK4 kinase activity.
• Compounds of the current invention are useful in the treatment of a proliferative disease selected from a benign or malignant tumor, solid tumor, carcinoma of the brain, kidney, liver, adrenal gland, bladder, breast, stomach, gastric tumors, ovaries, colon, rectum, prostate, pancreas, lung, vagina, cervix, testis, genitourinary tract, esophagus, larynx, skin, bone or thyroid, sarcoma, glioblastomas, neuroblastomas, multiple myeloma, gastrointestinal cancer, especially colon carcinoma or colorectal adenoma, a tumor of the neck and head, an epidermal hyperproliferation, psoriasis, prostate hyperplasia, a neoplasia, a neoplasia of epithelial character, adenoma, adenocarcinoma, keratoacanthoma, epidermoid carcinoma, large cell carcinoma, non
• the proliferative disease which can be treated according to the methods of this invention is an MyD88 driven disorder.
• the MyD88 driven disorder which can be treated according to the methods of this invention is selected from ABC DLBCL, primary CNS lymphomas, primary extranodal lymphomas, Waldenstrom’s macroglobulinemia, Hodgkin’s lymphoma, primary cutaneous T-cell lymphoma and chronic lymphocytic leukemia.
• the proliferative disease which can be treated according to the methods of this invention is an IL-1 driven disorder.
• the IL-1 driven disorder is Smoldering of indolent multiple myeloma.
• Compounds according to the invention are useful in the treatment of inflammatory or obstructive airways diseases, resulting, for example, in reduction of tissue damage, airways inflammation, bronchial hyperreactivity, remodeling or disease progression.
• Inflammatory or obstructive airways diseases to which the present invention is applicable include asthma of whatever type or genesis including both intrinsic (non-allergic) asthma and extrinsic (allergic) asthma, mild asthma, moderate asthma, severe asthma, bronchitic asthma, exercise-induced asthma, occupational asthma and asthma induced following bacterial infection.
• Treatment of asthma is also to be understood as embracing treatment of subjects, e.g. of less than 4 or 5 years of age, exhibiting wheezing symptoms and diagnosed or diagnosable as "whez infants", an established patient category of major medical concern and now often identified as incipient or early-phase asthmatics.
• Compounds according to the invention are useful in the treatment of heteroimmune diseases.
• heteroimmune diseases include, but are not limited to, graft versus host disease, transplantation, transfusion, anaphylaxis, allergies (e.g., allergies to plant pollens, latex, drugs, foods, insect poisons, animal hair, animal dander, dust mites, or cockroach calyx), type I hypersensitivity, allergic conjunctivitis, allergic rhinitis, and atopic dermatitis.
• Prophylactic efficacy in the treatment of asthma will be evidenced by reduced frequency or severity of symptomatic attack, e.g. of acute asthmatic or bronchoconstrictor attack, improvement in lung function or improved airways hyperreactivity. It may further be evidenced by reduced requirement for other, symptomatic therapy, such as therapy for or intended to restrict or abort symptomatic attack when it occurs, for example antiinflammatory or bronchodilatory.
• Prophylactic benefit in asthma may in particular be apparent in subjects prone to "morning dipping". "Morning dipping" is a recognized asthmatic syndrome, common to a substantial percentage of asthmatics and characterized by asthma attack, e.g. between the hours of about 4 to 6 am, i.e. at a time normally substantially distant form any previously administered symptomatic asthma therapy.
• Compounds of the current invention can be used for other inflammatory or obstructive airways diseases and conditions to which the present invention is applicable and include acute lung injury (ALI), adult/acute respiratory distress syndrome (ARDS), chronic obstructive pulmonary, airways or lung disease (COPD, COAD or COLD), including chronic bronchitis or dyspnea associated therewith, emphysema, as well as exacerbation of airways hyperreactivity consequent to other drug therapy, in particular other inhaled drug therapy.
• the invention is also applicable to the treatment of bronchitis of whatever type or genesis including, but not limited to, acute, arachidic, catarrhal, croupus, chronic or phthinoid bronchitis.
• pneumoconiosis an inflammatory, commonly occupational, disease of the lungs, frequently accompanied by airways obstruction, whether chronic or acute, and occasioned by repeated inhalation of dusts
• pneumoconiosis an inflammatory, commonly occupational, disease of the lungs, frequently accompanied by airways obstruction, whether chronic or acute, and occasioned by repeated inhalation of dusts
• aluminosis an inflammatory, commonly occupational, disease of the lungs, frequently accompanied by airways obstruction, whether chronic or acute, and occasioned by repeated inhalation of dusts
• aluminosis anthracosis
• asbestosis chalicosis
• ptilosis ptilosis
• siderosis silicosis
• tabacosis tabacosis and byssinosis.
• compounds of the invention are also useful in the treatment of eosinophil related disorders, e.g. eosinophilia, in particular eosinophil related disorders of the airways (e.g.
• eosinophilic infdtration of pulmonary tissues including hypereosinophilia as it effects the airways and/or lungs as well as, for example, eosinophil- related disorders of the airways consequential or concomitant to Loffler's syndrome, eosinophilic pneumonia, parasitic (in particular metazoan) infestation (including tropical eosinophilia), bronchopulmonary aspergillosis, polyarteritis nodosa (including Churg-Strauss syndrome), eosinophilic granuloma, eosinophilic asthma, eosinophilic COPD, and eosinophil-related disorders affecting the airways occasioned by drug-reaction.
• Compounds of the invention are also useful in the treatment of inflammatory or allergic conditions of the skin, for example psoriasis, generalized pustular psoriasis (GPP), psoriasis vulgaris, contact dermatitis, atopic dermatitis, alopecia areata, erythema multiforma, dermatitis herpetiformis, scleroderma, vitiligo, hypersensitivity angiitis, urticaria, bullous pemphigoid, lupus erythematosus, systemic lupus erythematosus, pemphigus vulgaris, pemphigus foliaceus, paraneoplastic pemphigus, epidermolysis bullosa acquisita, acne vulgaris, hidradenitis suppurativa, Sweet Syndrome, pyoderma gangrenosum, and other inflammatory or allergic conditions of the skin.
• GPP generalized pustular
• Compounds of the invention may also be used for the treatment of other diseases or conditions, such as diseases or conditions having an inflammatory component, for example, treatment of diseases and conditions of the eye such as ocular allergy, conjunctivitis, keratoconjunctivitis sicca, and vernal conjunctivitis, diseases affecting the nose including allergic rhinitis, and inflammatory disease in which autoimmune reactions are implicated or having an autoimmune component or etiology, including autoimmune hematological disorders (e.g.
• hemolytic anemia aplastic anemia, pure red cell anemia and idiopathic thrombocytopenia
• systemic lupus erythematosus rheumatoid arthritis, polychondritis, scleroderma, Wegener granulamatosis, dermatomyositis, chronic active hepatitis, myasthenia gravis, Steven-Johnson syndrome, idiopathic sprue, autoimmune inflammatory bowel disease (e.g.
• ulcerative colitis and Crohn's disease irritable bowel syndrome, celiac disease, periodontitis, hyaline membrane disease, kidney disease, glomerular disease, alcoholic liver disease, multiple sclerosis, endocrine opthalmopathy, Grave's disease, sarcoidosis, alveolitis, chronic hypersensitivity pneumonitis, multiple sclerosis, primary biliary cirrhosis, uveitis (anterior and posterior), Sjogren’s syndrome, keratoconjunctivitis sicca and vernal keratoconjunctivitis, interstitial lung fibrosis, psoriatic arthritis, systemic juvenile idiopathic arthritis, cryopyrin-associated periodic syndrome, nephritis, vasculitis, diverticulitis, interstitial cystitis, glomerulonephritis (with and without nephrotic syndrome, e.g.
• idiopathic nephrotic syndrome or minal change nephropathy including idiopathic nephrotic syndrome or minal change nephropathy), chronic granulomatous disease, endometriosis, leptospiriosis renal disease, glaucoma, retinal disease, ageing, headache, pain, complex regional pain syndrome, cardiac hypertrophy, musclewasting, catabolic disorders, obesity, fetal growth retardation, hyperchlolesterolemia, heart disease, chronic heart failure, mesothelioma, anhidrotic ecodermal dysplasia, Behcet’s disease, incontinentia pigmenti, Paget’s disease, pancreatitis, hereditary periodic fever syndrome, asthma (allergic and non-allergic, mild, moderate, severe, bronchitic, and exercise-induced), acute lung injury, acute respiratory distress syndrome, eosinophilia, hypersensitivities, anaphylaxis, nasal sinusitis, ocular allergy, silica induced diseases
• the inflammatory disease which can be treated according to the methods of this invention is an disease of the skin.
• the inflammatory disease of the skin is selected from contact dermatitits, atopic dermatitis, alopecia areata, erythema multiforma, dermatitis herpetiformis, scleroderma, vitiligo, hypersensitivity angiitis, urticaria, bullous pemphigoid, pemphigus vulgaris, pemphigus foliaceus, paraneoplastic pemphigus, epidermolysis bullosa acquisita, hidradenitis suppurativa, and other inflammatory or allergic conditions of the skin.
• the inflammatory disease which can be treated according to the methods of this invention is selected from acute and chronic gout, chronic gouty arthritis, psoriasis, psoriatic arthritis, rheumatoid arthritis, juvenile rheumatoid arthritis, systemic juvenile idiopathic arthritis (SJIA), cryopyrin associated periodic syndrome (CAPS), adult onset Still’s disease, macrophage activation syndrome (MAS), primary and secondary hemophagocytic lymphohistiocytosis (HLH), familial mediterranean fever, NLRP12 autoinflammatory syndrome, and osteoarthritis.
• SJIA systemic juvenile idiopathic arthritis
• CAS cryopyrin associated periodic syndrome
• MAS macrophage activation syndrome
• HH primary and secondary hemophagocytic lymphohistiocytosis
• familial mediterranean fever familial mediterranean fever
• NLRP12 autoinflammatory syndrome and osteoarthritis.
• the inflammatory disease which can be treated according to the methods of this invention is a TH17 mediated disease.
• the TH17 mediated disease is selected from Systemic lupus erythematosus, Multiple sclerosis, psoriasis vulgaris, hidradenitis suppurativa, and inflammatory bowel disease (including Crohn’s disease or ulcerative colitis).
• the inflammatory disease which can be treated according to the methods of this invention is selected from Sjogren’s syndrome, allergic disorders, osteoarthritis, conditions of the eye such as ocular allergy, conjunctivitis, keratoconjunctivitis sicca and vernal conjunctivitis, and diseases affecting the nose such as allergic rhinitis or chronic rhinosinusitis with nasal polyps (CRSwNP).
• Cardiovascular diseases which can be treated according to the methods of this invention include, but are not limited to, restenosis, cardiomegaly, atherosclerosis, myocardial infarction, ischemic stroke, congestive heart failure, angina pectoris, reocclusion after angioplasty, restenosis after angioplasty, reocclusion after aortocoronary bypass, restenosis after aortocoronary bypass, stroke, transitory ischemia, a peripheral arterial occlusive disorder, pulmonary embolism, and deep venous thrombosis.
• the neurodegenerative disease which can be treated according to the methods of this invention include, but are not limited to, Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, Huntington's disease, cerebral ischemia, and neurodegenerative disease caused by traumatic injury, glutamate neurotoxicity, hypoxia, epilepsy, treatment of diabetes, metabolic syndrome, obesity, organ transplantation and graft versus host disease.
• IRAK4 function results in decreased Ab levels in an in vivo murine model of Alzheimer’s disease and was associated with diminished microgliosis and astrogliosis in aged mice. Analysis of microglia isolated from the adult mouse brain revealed an altered pattern of gene expression associated with changes in microglial phenotype that were associated with expression of IRF transcription factors that govern microglial phenotype. Further, loss of IRAK4 function also promoted amyloid clearance mechanisms, including elevated expression of insulin-degrading enzyme. Finally, blocking IRAK function restored olfactory behavior (Cameron et al.
• the invention provides a method of treating, preventing or lessening the severity of Alzheimer’s disease comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt or composition thereof.
• the invention provides a method of treating a disease condition commonly occurring in connection with transplantation.
• the disease or condition commonly occurring in connection with transplantation is selected from organ transplantation, organ transplant rejection, and graft versus host disease.
• the invention provides a method of treating a metabolic disease.
• the metabolic disease is selected from Type 1 diabetes, Type 2 diabetes, metabolic syndrome, and obesity.
• the invention provides a method of treating a viral disease.
• the viral infection is HIV infection.
• the invention provides the use of a compound according to the definitions herein, or a pharmaceutically acceptable salt, or a hydrate or solvate thereof for the preparation of a medicament for the treatment of a proliferative disease, an inflammatory disease, an obstructive respiratory disease, a cardiovascular disease, a metabolic disease, a neurological disease, a neurodegenerative disease, a viral disease, or a disorder commonly occurring in connection with transplantation.
• the invention provides compounds that modulate targeted ubiquitination and degradation of one or more IRAK kinase.
• a provided compound modulates targeted ubiquitination and degradation of one or more IRAK kinase and one or more additional protein.
• a provided compound modulates targeted ubiquitination and degradation of IRAK4 and one, two, three, four, or five additional proteins.
• the invention provides compounds that are triple degraders.
• the invention provides compounds that combine IRAK kinase degradation with IKZF1 and IKZF3 degradation using IMiD-based IRAK degraders.
• E3 ligase ligands are thalidomide and its derivatives, lenalidomide and pomalidomide, commonly referred to as IMiDs (immunomodulatory imide drugs). These agents are small-molecule ligands of cereblon (CRBN) (Ito et al.
• Lenalidomide causes selective degradation of IKZF1 and IKZF3 in multiple myeloma cells” Science 2014, 343(6168):301-305; and Lu et al. “The myeloma drug lenalidomide promotes the cereblon-dependent destruction of Ikaros proteins” Science, 2014; 343(6168):305-309). This activity alone has potent antitumor effects in some liquid malignancies, and lenalidomide (Revlimid®) is US Food and Drug Administration approved for the treatment of MCL, multiple myeloma, and myelodysplastic syndromes with deletion of chromosome 5q. Lenalidomide is also undergoing late-stage clinical trials for a number of lymphomas, including MCL and the activated B-cell subtype of diffuse large B-cell lymphoma (ABC DLBCL).
• IRAK4 binding moieties coupled to non-IMiD CRBN binders mediate effective knockdown of IRAK4 but have little to no effect on the viability of MYD88 mutant ABC-DLBCL cell lines OCI-LY10 and SU-DHL- 2 in vitro.
• a non-IMiD-based degraders effects IRAK degradation in MYD88 mutant ABC DLBCL cell line tumor xenografts but without causing regression. This is consistent with literature demonstrating no effect on growth of OCI-LYIO or other MYD88 mutant lines when the gene encoding IRAK4 is removed at the DNA level using CRISPR/Cas9 editing (Phelan et al. “A multiprotein supercomplex controlling oncogenic signaling in lymphoma” Nature, 2018, 7718:387-391).
• IMiDs are also known to increase the IFN response in MYD88 mutant ABC-DLBCL to levels sufficient to increase apoptosis (Yang, Cancer Cell 2012; and Hagner et al. “CC- 122, a pleiotropic pathway modifier, mimics an interferon response and has antitumor activity in DLBCL” Blood 2015, 126:779-789). This effect has been shown to synergize with inhibition of NFkB signaling to further drive DLBCL cell death (Yang, Cancer Cell 2012).
• the combination of an IMiD with a small molecule IRAK4 kinase inhibitor shows little to no additive effect on viability of the MYD88 mutant ABC DLBCL cell lines, such as OCI- LYIO.
• the combination of an IRAK4 inhibitor with IMiD is less active than an all- in-one IMiD-based IRAK4 degrader.
• the combination of IRAK kinase degradation with IKZF1 and IKZF3 degradation in an all-in-one IMiD-based IRAK4 degrader shows potent, single agent activity versus MYD88 mutant ABC DLBCL cell lines in vitro and OCI-LYIO xenograft in vivo.
• an all-in-one combination of an IMiD-based CRBN-binder and an IRAK4 binding moiety yields IRAK4 degraders that retain degradation of Ikaros (IKZF1) and other known IMiDs neosubstrates, while more strongly inducing an interferon response compared to pomalidomide alone.
• IMiD- based IRAK4 degraders are potent at killing MYD88 mutant ABD-DLBCL cell lines in vitro, demonstrating increased activity versus that obtained from combining an IRAK4 inhibitor with IMiDs as single agents.
• a provided compound comprising an IMiD-based E3 ligase degrades IRAK4, Ikaros, and Aiolos in MYD88 mutant ABC DLBCL cell line xenografts in vivo, and strongly induces a signature of interferon-driven proteins exemplified by IFIT1 (interferon-inducible transcript 1) and IFIT3 (interferon-inducible transcript 3).
• a provided compound comprising an IMiD-based E3 ligase drives regression of tumor xenographs as a single agent.
• the provided compounds of present invention highlight a synergy obtained by combining IRAK4 degradation with IMiD induction of interferon response to drive single agent anti-tumor activity in MYD88 mutant DLBCL and possibly in other heme malignancies.
• a provided compound comprising an IMiD-based E3 ligase degrade IRAK4, Ikaros, and Aiolos acts synergistically.
• a provided compound comprising an IRAK4 binder and an IMiD-based E3 ligase degrades IRAK4, Ikaros, and Aiolos with increased activity in comparison to a provided compound comprising the same IRAK4 binder and a non-IMiD-based E3 ligase and the same IMiD-based E3 ligase as a single agent.
• the present invention provides a method of treating solid and liquid tumors in a patient in need thereof, comprising administering a compound of the present invention, or a pharmaceutically acceptable salt thereof.
• the present invention provides a method of treating MYD88-mutant Waldenstrom macroglobulinemia in a patient in need thereof, comprising administering a compound of the present invention, or a pharmaceutically acceptable salt thereof.
• the present invention provides a method of treating a AML, or a subset thereof, in a patient in need thereof, comprising administering a compound of the present invention, or a pharmaceutically acceptable salt thereof.
• the present invention provides a method of treating NSCLC in a patient in need thereof, comprising administering a compound of the present invention, or a pharmaceutically acceptable salt thereof.
• additional therapeutic agents which are normally administered to treat that condition, may be administered in combination with compounds and compositions of this invention.
• additional therapeutic agents that are normally administered to treat a particular disease, or condition are known as “appropriate for the disease, or condition, being treated.”
• a provided combination, or composition thereof is administered in combination with another therapeutic agent.
• the present invention provides a method of treating a disclosed disease or condition comprising administering to a patient in need thereof an effective amount of a compound disclosed herein or a pharmaceutically acceptable salt thereof and co-administering simultaneously or sequentially an effective amount of one or more additional therapeutic agents, such as those described herein.
• the method includes co-administering one additional therapeutic agent.
• the method includes co-administering two additional therapeutic agents.
• the combination of the disclosed compound and the additional therapeutic agent or agents acts synergistically.
• MS Multiple
• combination therapies of the present invention are administered in combination with a monoclonal antibody or an siRNA therapeutic.
• Those additional agents may be administered separately from a provided combination therapy, as part of a multiple dosage regimen.
• those agents may be part of a single dosage form, mixed together with a compound of this invention in a single composition. If administered as part of a multiple dosage regime, the two active agents may be submitted simultaneously, sequentially or within a period of time from one another normally within five hours from one another.
• the term “combination,” “combined,” and related terms refers to the simultaneous or sequential administration of therapeutic agents in accordance with this invention.
• a combination of the present invention may be administered with another therapeutic agent simultaneously or sequentially in separate unit dosage forms or together in a single unit dosage form.
• the amount of additional therapeutic agent present in the compositions of this invention will be no more than the amount that would normally be administered in a composition comprising that therapeutic agent as the only active agent.
• the amount of additional therapeutic agent in the presently disclosed compositions will range from about 50% to 100% of the amount normally present in a composition comprising that agent as the only therapeutically active agent.
• One or more other therapeutic agent may be administered separately from a compound or composition of the invention, as part of a multiple dosage regimen.
• one or more other therapeutic agents may be part of a single dosage form, mixed together with a compound of this invention in a single composition.
• one or more other therapeutic agent and a compound or composition of the invention may be administered simultaneously, sequentially or within a period of time from one another, for example within 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 18, 20, 21, 22, 23, or 24 hours from one another.
• one or more other therapeutic agent and a compound or composition of the invention are administered as a multiple dosage regimen within greater than 24 hours apart.
• the present invention provides a composition comprising a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents.
• the therapeutic agent may be administered together with a provided compound or a pharmaceutically acceptable salt thereof, or may be administered prior to or following administration of a provided compound or a pharmaceutically acceptable salt thereof. Suitable therapeutic agents are described in further detail below.
• a provided compound or a pharmaceutically acceptable salt thereof may be administered up to 5 minutes, 10 minutes, 15 minutes, 30 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 5, hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, 11 hours, 12 hours, 13 hours, 14 hours, 15 hours, 16 hours, 17 hours, or 18 hours before the therapeutic agent.
• a provided compound or a pharmaceutically acceptable salt thereof may be administered up to 5 minutes, 10 minutes, 15 minutes, 30 minutes, 1 hour, 2 hours, 3 hours, 4 hours, 5, hours, 6 hours, 7 hours, 8 hours, 9 hours, 10 hours, 11 hours, 12 hours, 13 hours, 14 hours, 15 hours, 16 hours, 17 hours, or 18 hours following the therapeutic agent.
• the present invention provides a method of treating an inflammatory disease, disorder or condition by administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents.
• additional therapeutic agents may be small molecules or recombinant biologic agents and include, for example, acetaminophen, non-steroidal anti-inflammatory drugs (NSAIDS) such as aspirin, ibuprofen, naproxen, etodolac (Lodine®) and celecoxib, colchicine (Colcrys®), corticosteroids such as prednisone, prednisolone, methylprednisolone, hydrocortisone, and the like, probenecid, allopurinol, febuxostat (Uloric®), sulfasalazine (Azulfidine®), antimalarials such as hydroxychloroquine (Plaquenil®) and chloroquine (Aralen
• NSAIDS non
• the present invention provides a method of treating gout comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from non-steroidal anti-inflammatory drugs (N SAIDS) such as aspirin, ibuprofen, naproxen, etodolac (Lodine®) and celecoxib, colchicine (Colcrys®), corticosteroids such as prednisone, prednisolone, methylprednisolone, hydrocortisone, and the like, probenecid, allopurinol and febuxostat (Uloric®).
• N SAIDS non-steroidal anti-inflammatory drugs
• ibuprofen such as aspirin, ibuprofen, naproxen, etodolac (Lodine®) and celecoxib
• colchicine Colderrys®
• corticosteroids such as prednisone, prednisolone,
• the present invention provides a method of treating rheumatoid arthritis comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from non-steroidal anti inflammatory drugs (N SAIDS) such as aspirin, ibuprofen, naproxen, etodolac (Lodine®) and celecoxib, corticosteroids such as prednisone, prednisolone, methylprednisolone, hydrocortisone, and the like, sulfasalazine (Azulfidine®), antimalarials such as hydroxychloroquine (Plaquenil®) and chloroquine (Aralen®), methotrexate (Rheumatrex®), gold salts such as gold thioglucose (Solganal®), gold thiomalate (Myochrysine®) and auranofm (Rid
• N SAIDS non-ster
• the present invention provides a method of treating osteoarthritis comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from acetaminophen, non steroidal anti-inflammatory drugs (NSAIDS) such as aspirin, ibuprofen, naproxen, etodolac (Lodine®) and celecoxib, diclofenac, cortisone, hyaluronic acid (Synvisc® or Hyalgan®) and monoclonal antibodies such as tanezumab.
• NSAIDS non steroidal anti-inflammatory drugs
• the present invention provides a method of treating lupus comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from acetaminophen, non-steroidal anti inflammatory drugs (NSAIDS) such as aspirin, ibuprofen, naproxen, etodolac (Lodine®) and celecoxib, corticosteroids such as prednisone, prednisolone, methylprednisolone, hydrocortisone, and the like, antimalarials such as hydroxychloroquine (Plaquenil®) and chloroquine (Aralen®), cyclophosphamide (Cytoxan®), methotrexate (Rheumatrex®), azathioprine (Imuran®) and anticoagulants such as heparin (Calcinparine® or Liquaemin®) and warfarin (Co
• NSAIDS non-steroidal
• the present invention provides a method of treating inflammatory bowel disease comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from mesalamine (Asacol®) sulfasalazine (Azulfidine®), antidiarrheals such as diphenoxylate (Lomotil®) and loperamide (Imodium®), bile acid binding agents such as cholestyramine, alosetron (Lotronex®), lubiprostone (Amitiza®), laxatives such as Milk of Magnesia, polyethylene glycol (MiraLax®), Dulcolax®, Correctol® and Senokot® and anticholinergics or antispasmodics such as dicyclomine (Bentyl®), anti-TNF therapies, steroids, and antibiotics such as Flagyl or ciprofloxacin.
• mesalamine Asacol®
• Amulfidine® anti
• the present invention provides a method of treating asthma comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from anti-IL-33 antibodies such as REGN3500 (SAR440340) or CNTO 7160, Singulair®, beta-2 agonists such as albuterol (Ventolin® HFA, Proventil® HFA), levalbuterol (Xopenex®), metaproterenol (Alupent®), pirbuterol acetate (Maxair®), terbutaline sulfate (Brethaire®), salmeterol xinafoate (Serevent®) and formoterol (Foradil®), anticholinergic agents such as ipratropium bromide (Atrovent®) and tiotropium (Spiriva®), inhaled corticosteroids such as prednisone, prednisolone, beclomethasone di
• the present invention provides a method of treating COPD comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from beta-2 agonists such as albuterol (Ventolin® HFA, Proventil® HFA), levalbuterol (Xopenex®), metaproterenol (Alupent®), pirbuterol acetate (Maxair®), terbutaline sulfate (Brethaire®), salmeterol xinafoate (Serevent®) and formoterol (Foradil®), anticholinergic agents such as ipratropium bromide (Atrovent®) and tiotropium (Spiriva®), methylxanthines such as theophylline (Theo-Dur®, Theolair®, Slo-bid®, Uniphyl®, Theo-24®) and aminophylline, inhaled corticosteroids such as
• the present invention provides amethod of treating eosinophilic asthma comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from an anti-IL-33 antibody such as REGN3500 (SAR440340) or CNTO 7160.
• an anti-IL-33 antibody such as REGN3500 (SAR440340) or CNTO 7160.
• the present invention provides a method of treating HIV comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from nucleoside reverse transcriptase inhibitors such as zidovudine (Retrovir®), abacavir (Ziagen®), abacavir/lamivudine (Epzicom®), abacavir/lamivudine/zidovudine (Trizivir®), didanosine (Videx®), emtricitabine (Emtriva®), lamivudine (Epivir®), lamivudine/zidovudine (Combivir®), stavudine (Zerit®), and zalcitabine (Hivid®), non nucleoside reverse transcriptase inhibitors such as delavirdine (Rescriptor®), efavirenz (Sustiva®), nevairapine (Vir), abacavir (Zi
• the present invention provides a method of treating a hematological malignancy comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from rituximab (Rituxan®), cyclophosphamide (Cytoxan®), doxorubicin (Hydrodaunorubicin®), vincristine (Oncovin®), prednisone, a hedgehog signaling inhibitor, a BTK inhibitor, a JAK/pan-JAK inhibitor, a TYK2 inhibitor, a PI3K inhibitor, a SYK inhibitor, and combinations thereof.
• additional therapeutic agents selected from rituximab (Rituxan®), cyclophosphamide (Cytoxan®), doxorubicin (Hydrodaunorubicin®), vincristine (Oncovin®), prednisone, a hedgehog signaling inhibitor, a BTK inhibitor,
• the present invention provides a method of treating a solid tumor comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from rituximab (Rituxan®), cyclophosphamide (Cytoxan®), doxorubicin (Hydrodaunorubicin®), vincristine (Oncovin®), prednisone, a hedgehog signaling inhibitor, a BTK inhibitor, a JAK/pan-JAK inhibitor, a TYK2 inhibitor, a PI3K inhibitor, a SYK inhibitor, and combinations thereof.
• additional therapeutic agents selected from rituximab (Rituxan®), cyclophosphamide (Cytoxan®), doxorubicin (Hydrodaunorubicin®), vincristine (Oncovin®), prednisone, a hedgehog signaling inhibitor, a BTK inhibitor, a JAK/pan
• the present invention provides a method of treating a hematological malignancy comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a Hedgehog (Hh) signaling pathway inhibitor.
• the hematological malignancy is DLBCL (Ramirez et al “Defining causative factors contributing in the activation of hedgehog signaling in diffuse large B-cell lymphoma” Leuk. Res. (2012), published online July 17, and incorporated herein by reference in its entirety).
• the present invention provides a method of treating difluse large B- cell lymphoma (DLBCL) comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from rituximab (Rituxan®), cyclophosphamide (Cytoxan®), doxorubicin (Hydrodaunorubicin®), vincristine (Oncovin®), prednisone, a hedgehog signaling inhibitor, and combinations thereof.
• rituximab Renituxan®
• Cytoxan® cyclophosphamide
• doxorubicin Hydrodaunorubicin®
• vincristine Oncovin®
• prednisone a hedgehog signaling inhibitor
• the present invention provides a method of treating DLBCL comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a CHOP (cyclophosphamide, Hydrodaunorubicin®, Oncovin®, and prednisone or prednisolone) or R-CHOP (rituximab, cyclophosphamide, Hydrodaunorubicin®, Oncovin®, and prednisone or prednisolone) chemotherapy regimen.
• CHOP cyclophosphamide, Hydrodaunorubicin®, Oncovin®, and prednisone or prednisolone
• R-CHOP rituximab, cyclophosphamide, Hydrodaunorubicin®, Oncovin®, and prednisone or prednisolone
• the present invention provides a method of treating DLBCL comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a rituximab/bendamustine chemotherapy regimen.
• the present invention provides a method of treating DLBCL comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a BTK inhibitor (e.g., ibrutinib).
• the present invention provides a method of treating DLBCL comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and an anti-CD20 antibody (e.g., rituximab).
• an anti-CD20 antibody e.g., rituximab
• the present invention provides a method of treating DLBCL comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and an anti-CD79B ADC (e.g., polatuzumab).
• a provided compound or a pharmaceutically acceptable salt thereof e.g., polatuzumab.
• an anti-CD79B ADC e.g., polatuzumab
• the present invention provides a method of treating DLBCL comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a BCL2 inhibitor (e.g., venetoclax).
• a BCL2 inhibitor e.g., venetoclax
• the present invention provides a method of treating DLBCL comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and lenalidomide or pomalidomide
• the present invention provides a method of treating DLBCL comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a PI3K inhibitor (e.g., umbralisib).
• a PI3K inhibitor e.g., umbralisib
• the present invention provides a method of treating a T-cell disease or deficiency describing herein comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a PI3K inhibitor (e.g., umbralisib).
• a PI3K inhibitor e.g., umbralisib
• the present invention provides a method of treating DLBCL comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a protesome inhibitor (e.g., bortezomib)
• a protesome inhibitor e.g., bortezomib
• the present invention provides a method of treating a T-cell disease or deficiency describing herein comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a protesome inhibitor (e.g., bortezomib).
• a protesome inhibitor e.g., bortezomib
• the present invention provides a method of treating multiple myeloma comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from bortezomib (Velcade®), and dexamethasone (Decadron®), a hedgehog signaling inhibitor, a BTK inhibitor, a JAK/pan-JAK inhibitor, a TYK2 inhibitor, a PI3K inhibitor, a SYK inhibitor in combination with lenalidomide (Revlimid®).
• additional therapeutic agents selected from bortezomib (Velcade®), and dexamethasone (Decadron®), a hedgehog signaling inhibitor, a BTK inhibitor, a JAK/pan-JAK inhibitor, a TYK2 inhibitor, a PI3K inhibitor, a SYK inhibitor in combination with lenalidomide (Revlimid®).
• the present invention provides a method of treating Waldenstrom’s macroglobulinemia comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from chlorambucil (Leukeran®), cyclophosphamide (Cytoxan®, Neosar®), fludarabine (Fludara®), cladribine (Leustatin®), rituximab (Rituxan®), a hedgehog signaling inhibitor, a BTK inhibitor, a JAK/pan-JAK inhibitor, a TYK2 inhibitor, a PI3K inhibitor, and a SYK inhibitor.
• additional therapeutic agents selected from chlorambucil (Leukeran®), cyclophosphamide (Cytoxan®, Neosar®), fludarabine (Fludara®), cladribine (Leustatin®), rituximab (Rituxan®), a hedgehog signal
• one or more other therapeutic agent is an antagonist of the hedgehog pathway.
• Approved hedgehog pathway inhibitors which may be used in the present invention include sonidegib (Odomzo®, Sun Pharmaceuticals); and vismodegib (Erivedge®, Genentech), both for treatment of basal cell carcinoma.
• one or more other therapeutic agent is a Poly ADP ribose polymerase (PARP) inhibitor.
• PARP Poly ADP ribose polymerase
• a PARP inhibitor is selected from olaparib (Lynparza®, AstraZeneca); rucaparib (Rubraca®, Clovis Oncology); niraparib (Zejula®, Tesaro); talazoparib (MDV3800/BMN 673/LT00673, Medivation/Pfizer/Biomarin); veliparib (ABT-888, Abb Vie); and BGB- 290 (BeiGene, Inc.).
• one or more other therapeutic agent is a histone deacetylase (HDAC) inhibitor.
• HDAC histone deacetylase
• an HDAC inhibitor is selected from vorinostat (Zolinza®, Merck); romidepsin (Istodax®, Celgene); panobinostat (Farydak®, Novartis); belinostat (Beleodaq®, Spectrum Pharmaceuticals); entinostat (SNDX-275, Syndax Pharmaceuticals) (NCT00866333); and chidamide (Epidaza®, HBI-8000, Chipscreen Biosciences, China).
• one or more other therapeutic agent is a CDK inhibitor, such as a CDK4/CDK6 inhibitor.
• a CDK 4/6 inhibitor is selected from palbociclib (Ibrance®, Pfizer); ribociclib (Kisqali®, Novartis); abemaciclib (Ly2835219, Eli Lilly); and trilaciclib (G1T28, G1 Therapeutics).
• one or more other therapeutic agent is a folic acid inhibitor.
• Approved folic acid inhibitors useful in the present invention include pemetrexed (Alimta®, Eli Lilly).
• one or more other therapeutic agent is a CC chemokine receptor 4 (CCR4) inhibitor.
• CCR4 inhibitors being studied that may be useful in the present invention include mogamulizumab (Poteligeo®, KyowaHakko Kirin, Japan).
• one or more other therapeutic agent is an isocitrate dehydrogenase (IDH) inhibitor.
• IDH inhibitors being studied which may be used in the present invention include AG 120 (Celgene; NCT02677922); AG221 (Celgene, NCT02677922; NCT02577406); BAY1436032 (Bayer, NCT02746081); IDH305 (Novartis, NCT02987010).
• one or more other therapeutic agent is an arginase inhibitor.
• Arginase inhibitors being studied which may be used in the present invention include AEB1102 (pegylated recombinant arginase, Aeglea Biotherapeutics), which is being studied in Phase 1 clinical trials for acute myeloid leukemia and myelodysplastic syndrome (NCT02732184) and solid tumors (NCT02561234); and CB-1158 (Calithera Biosciences).
• AEB1102 pegylated recombinant arginase, Aeglea Biotherapeutics
• NCT02732184 myelodysplastic syndrome
• NCT02561234 solid tumors
• CB-1158 Calithera Biosciences
• one or more other therapeutic agent is a glutaminase inhibitor.
• Glutaminase inhibitors being studied which may be used in the present invention include CB-839 (Calithera Biosciences).
• one or more other therapeutic agent is an antibody that binds to tumor antigens, that is, proteins expressed on the cell surface of tumor cells.
• Approved antibodies that bind to tumor antigens which may be used in the present invention include rituximab (Rituxan®, Genentech/Biogenldec); ofatumumab (anti-CD20, Arzerra®, GlaxoSmithKline); obinutuzumab (anti- CD20, Gazyva®, Genentech), ibritumomab (anti-CD20 and Yttrium-90, Zevalin®, Spectrum Pharmaceuticals); daratumumab (anti-CD38, Darzalex®, Janssen Biotech), dinutuximab (anti-glycolipid GD2, Unituxin®, United Therapeutics); trastuzumab (anti-HER2, Herceptin®, Genentech); ado- trastuzumab emtansine (anti-
• one or more other therapeutic agent is a topoisomerase inhibitor.
• Approved topoisomerase inhibitors useful in the present invention include irinotecan (Onivyde®, Merrimack Pharmaceuticals); topotecan (Hycamtin®, GlaxoSmithKline).
• Topoisomerase inhibitors being studied which may be used in the present invention include pixantrone (Pixuvri®, CTI Biopharma).
• one or more other therapeutic agent is an inhibitor of anti-apoptotic proteins, such as BCL-2.
• Approved anti-apoptotics which may be used in the present invention include venetoclax (Venclexta®, AbbVie/Genentech); and blinatumomab (Blincyto®, Amgen).
• Other therapeutic agents targeting apoptotic proteins which have undergone clinical testing and may be used in the present invention include navitoclax (ABT-263, Abbott), a BCL-2 inhibitor (NCT02079740).
• one or more other therapeutic agent is an androgen receptor inhibitor.
• Approved androgen receptor inhibitors useful in the present invention include enzalutamide (Xtandi®, Astellas/Medivation); approved inhibitors of androgen synthesis include abiraterone (Zytiga®, Centocor/Ortho); approved antagonist of gonadotropin-releasing hormone (GnRH) receptor (degaralix, Firmagon®, Ferring Pharmaceuticals).
• one or more other therapeutic agent is a selective estrogen receptor modulator (SERM), which interferes with the synthesis or activity of estrogens.
• Approved SERMs useful in the present invention include raloxifene (Evista®, Eli Lilly).
• one or more other therapeutic agent is an inhibitor of bone resorption.
• An approved therapeutic which inhibits bone resorption is Denosumab (Xgeva®, Amgen), an antibody that binds to RANKL, prevents binding to its receptor RANK, found on the surface of osteoclasts, their precursors, and osteoclast-like giant cells, which mediates bone pathology in solid tumors with osseous metastases.
• Other approved therapeutics that inhibit bone resorption include bisphosphonates, such as zoledronic acid (Zometa®, Novartis).
• one or more other therapeutic agent is an inhibitor of interaction between the two primary p53 suppressor proteins, MDMX and MDM2.
• Inhibitors of p53 suppression proteins being studied which may be used in the present invention include ALRN-6924 (Aileron), a stapled peptide that equipotently binds to and disrupts the interaction of MDMX and MDM2 with p53.
• ALRN- 6924 is currently being evaluated in clinical trials for the treatment of AML, advanced myelodysplastic syndrome (MDS) and peripheral T-cell lymphoma (PTCL) (NCT02909972; NCT02264613).
• one or more other therapeutic agent is an inhibitor of transforming growth factor-beta (TGF-beta or TGFB).
• TGF-beta or TGFB transforming growth factor-beta
• Inhibitors of TGF-beta proteins being studied which may be used in the present invention include NIS793 (Novartis), an anti-TGF-beta antibody being tested in the clinic for treatment of various cancers, including breast, lung, hepatocellular, colorectal, pancreatic, prostate and renal cancer (NCT 02947165).
• the inhibitor of TGF-beta proteins is fresolimumab (GC1008; Sanofi-Genzyme), which is being studied for melanoma (NCT00923169); renal cell carcinoma (NCT00356460); and non-small cell lung cancer (NCT02581787).
• the additional therapeutic agent is a TGF-beta trap, such as described in Connolly et al. (2012) Int’l J. Biological Sciences 8:964-978.
• M7824 (Merck KgaA - formerly MSB0011459X), which is a bispecific, anti-PD-L l/TGFB trap compound (NCT02699515); and (NCT02517398).
• M7824 is comprised of a fully human IgGl antibody against PD-L1 fused to the extracellular domain of human TGF-beta receptor II, which functions as a TGFB “trap.”
• one or more other therapeutic agent is selected from glembatumumab vedotin-monomethyl auristatin E (MMAE) (Celldex), an anti-glycoprotein NMB (gpNMB) antibody (CR011) linked to the cytotoxic MMAE.
• MMAE glembatumumab vedotin-monomethyl auristatin E
• gpNMB anti-glycoprotein NMB
• gpNMB is a protein overexpressed by multiple tumor types associated with cancer cells’ ability to metastasize.
• one or more other therapeutic agent is an antiproliferative compound.
• antiproliferative compounds include, but are not limited to aromatase inhibitors; antiestrogens; topoisomerase I inhibitors; topoisomerase II inhibitors; microtubule active compounds; alkylating compounds; histone deacetylase inhibitors; compounds which induce cell differentiation processes; cyclooxygenase inhibitors; MMP inhibitors; mTOR inhibitors; antineoplastic antimetabolites; platin compounds; compounds targeting/decreasing a protein or lipid kinase activity and further anti-angiogenic compounds; compounds which target, decrease or inhibit the activity of a protein or lipid phosphatase; gonadorelin agonists; anti -androgens; methionine aminopeptidase inhibitors; matrix metalloproteinase inhibitors; bisphosphonates; biological response modifiers; antiproliferative antibodies; heparanase inhibitors; inhibitors
• the present invention provides a method of treating Alzheimer’s disease comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from donepezil (Aricept ® ), rivastigmine (Excelon ® ), galantamine (Razadyne ® ), tacrine (Cognex ® ), and memantine (Namenda ® ).
• one or more other therapeutic agent is a taxane compound, which causes disruption of microtubules, which are essential for cell division.
• a taxane compound is selected from paclitaxel (Taxol®, Bristol-Myers Squibb), docetaxel (Taxotere®, Sanofi-Aventis; Docefrez®, Sun Pharmaceutical), albumin-bound paclitaxel (Abraxane®; Abraxis/Celgene), cabazitaxel (Jevtana®, Sanofi-Aventis), and SID530 (SK Chemicals, Co.) (NCT00931008).
• paclitaxel Texol®, Bristol-Myers Squibb
• docetaxel Taxotere®, Sanofi-Aventis
• Docefrez® Sun Pharmaceutical
• albumin-bound paclitaxel Abraxane®; Abraxis/Celgene
• cabazitaxel Jevtana®, Sanofi-Aventis
• SID530 SK Chemicals, Co.
• one or more other therapeutic agent is a nucleoside inhibitor, or a therapeutic agent that interferes with normal DNA synthesis, protein synthesis, cell replication, or will otherwise inhibit rapidly proliferating cells.
• a nucleoside inhibitor is selected from trabectedin (guanidine alkylating agent, Yondelis®, Janssen Oncology), mechlorethamine (alkylating agent, Valchlor®, Aktelion Pharmaceuticals); vincristine (Oncovin®, Eli Lilly; Vincasar®, Teva Pharmaceuticals; Marqibo®, Talon Therapeutics); temozolomide (prodrug to alkylating agent 5-(3-methyltriazen-l-yl)-imidazole-4- carboxamide (MTIC) Temodar®, Merck); cytarabine injection (ara-C, antimetabolic cytidine analog, Pfizer); lomustine (alkylating agent, CeeNU®, Bristol-Myers Squibb; Gleostine®, NextSource Biotechnology); azacitidine (pyrimidine nucleoside analog of cytidine, Vidaza®, Celgene); omacetaxine me
• one or more other therapeutic agent is a kinase inhibitor or VEGF-R antagonist.
• Approved VEGF inhibitors and kinase inhibitors useful in the present invention include: bevacizumab (Avastin®, Genentech/Roche) an anti-VEGF monoclonal antibody; ramucirumab (Cyramza®, Eli Lilly), an anti-VEGFR-2 antibody and ziv-aflibercept, also known as VEGF Trap (Zaltrap®; Regeneron/Sanofi).
• VEGFR inhibitors such as regorafenib (Stivarga®, Bayer); vandetanib (Caprelsa®, AstraZeneca); axitinib (Inlyta®, Pfizer); and lenvatinib (Lenvima®, Eisai); Raf inhibitors, such as sorafenib (Nexavar®, Bayer AG and Onyx); dabrafenib (Tafmlar®, Novartis); and vemurafenib (Zelboraf®, Genentech/Roche); MEK inhibitors, such as cobimetanib (Cotellic®, Exelexis/Genentech/Roche); trametinib (Mekinist®, Novartis); Bcr-Abl tyrosine kinase inhibitors, such as imatinib (Gleevec®, Novartis); nilotinib (Tasigna®, Nov
• kinase inhibitors and VEGF-R antagonists that are in development and may be used in the present invention include tivozanib (Aveo Pharmaecuticals); vatalanib (Bayer/Novartis); lucitanib (Clovis Oncology); dovitinib (TKI258, Novartis); Chiauanib (Chipscreen Biosciences); CEP-11981 (Cephalon); linifanib (Abbott Laboratories); neratinib (HKI-272, Puma Biotechnology); radotinib (Supect®, IY5511, Il-Yang Pharmaceuticals, S.
• ruxolitinib Jakafi®, Incyte Corporation
• PTC299 PTC Therapeutics
• CP-547,632 Pfizer
• foretinib Exelexis, GlaxoSmithKline
• quizartinib Daiichi Sankyo
• motesanib Amgen/Takeda
• the present invention provides a method of treating organ transplant rejection or graft vs. host disease comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from a steroid, cyclosporin, FK506, rapamycin, a hedgehog signaling inhibitor, a BTK inhibitor, a JAK/pan-JAK inhibitor, a TYK2 inhibitor, a PI3K inhibitor, and a SYK inhibitor.
• additional therapeutic agents selected from a steroid, cyclosporin, FK506, rapamycin, a hedgehog signaling inhibitor, a BTK inhibitor, a JAK/pan-JAK inhibitor, a TYK2 inhibitor, a PI3K inhibitor, and a SYK inhibitor.
• the present invention provides a method of treating or lessening the severity of a disease comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a BTK inhibitor, wherein the disease is selected from inflammatory bowel disease, arthritis, systemic lupus erythematosus (SLE), vasculitis, idiopathic thrombocytopenic purpura (ITP), rheumatoid arthritis, psoriatic arthritis, osteoarthritis, Still’s disease, juvenile arthritis, diabetes, myasthenia gravis, Hashimoto’s thyroiditis, Ord’s thyroiditis, Graves’ disease, autoimmune thyroiditis, Sjogren’s syndrome, multiple sclerosis, systemic sclerosis, Lyme neuroborreliosis, Guillain-Barre syndrome, acute disseminated encephalomyelitis, Addison’s disease, opsoclonus- myoclonus syndrome, anky
• the present invention provides a method of treating or lessening the severity of a disease comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a PI3K inhibitor, wherein the disease is selected from a cancer, a neurodegenative disorder, an angiogenic disorder, a viral disease, an autoimmune disease, an inflammatory disorder, a hormone-related disease, conditions associated with organ transplantation, immunodeficiency disorders, a destructive bone disorder, a proliferative disorder, an infectious disease, a condition associated with cell death, thrombin-induced platelet aggregation, chronic myelogenous leukemia (CML), chronic lymphocytic leukemia (CLL), liver disease, pathologic immune conditions involving T cell activation, a cardiovascular disorder, and a CNS disorder.
• the disease is selected from a cancer, a neurodegenative disorder, an angiogenic disorder, a viral disease, an autoimmune disease, an inflammatory disorder, a hormone-related disease, conditions associated
• the present invention provides a method of treating or lessening the severity of a disease comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and a PI3K inhibitor, wherein the disease is selected from benign or malignant tumor, carcinoma or solid tumor of the brain, kidney (e.g., renal cell carcinoma (RCC)), liver, adrenal gland, bladder, breast, stomach, gastric tumors, ovaries, colon, rectum, prostate, pancreas, lung, vagina, endometrium, cervix, testis, genitourinary tract, esophagus, larynx, skin, bone or thyroid, sarcoma, glioblastomas, neuroblastomas, multiple myeloma or gastrointestinal cancer, especially colon carcinoma or colorectal adenoma or a tumor of the neck and head, an epidermal hyperproliferation, psoriasis, prostate hyperplasia, a neo
• hemolytic anemia aplastic anemia, pure red cell anemia and idiopathic thrombocytopenia
• systemic lupus erythematosus rheumatoid arthritis, polychondritis, sclerodoma, Wegener granulamatosis, dermatomyositis, chronic active hepatitis, myasthenia gravis, Steven-Johnson syndrome, idiopathic sprue, autoimmune inflammatory bowel disease (e.g.
• ulcerative colitis and Crohn's disease endocrine opthalmopathy
• Grave's disease sarcoidosis, alveolitis, chronic hypersensitivity pneumonitis, multiple sclerosis, primary biliary cirrhosis, uveitis (anterior and posterior), keratoconjunctivitis sicca and vernal keratoconjunctivitis, interstitial lung fibrosis, psoriatic arthritis and glomerulonephritis (with and without nephrotic syndrome, e.g.
• idiopathic nephrotic syndrome or minal change nephropathy, restenosis, cardiomegaly, atherosclerosis, myocardial infarction, ischemic stroke and congestive heart failure, Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, Huntington's disease, and cerebral ischemia, and neurodegenerative disease caused by traumatic injury, glutamate neurotoxicity and hypoxia.
• one or more other therapeutic agent is a phosphatidylinositol 3 kinase (PI3K) inhibitor.
• PI3K inhibitor is selected from idelalisib (Zydelig®, Gilead), alpelisib (BYL719, Novartis), taselisib (GDC-0032, Genentech/Roche); pictilisib (GDC-0941, Genentech/Roche); copanlisib (BAY806946, Bayer); duvelisib (formerly IPI-145, Infinity Pharmaceuticals); PQR309 (Piqur Therapeutics, Switzerland); and TGR1202 (formerly RP5230, TG Therapeutics).
• the present invention provides a method of treating AML comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from: FLT3 inhibitors; targeted agents such as IDH inhibitors, anti-CD33 ADCs (e.g. Mylotarg), BCL2 inhibitors, and Hedgehog inhibitors; and chemotherapy such as AraC, daunarubicin, etoposide, methotrexate, fludarabine, mitozantrone, azacytidine, and corticosteroids.
• FLT3 inhibitors targeted agents such as IDH inhibitors, anti-CD33 ADCs (e.g. Mylotarg), BCL2 inhibitors, and Hedgehog inhibitors
• chemotherapy such as AraC, daunarubicin, etoposide, methotrexate, fludarabine, mitozantrone, azacytidine, and corticosteroids.
• the present invention provides a method of treating MDS comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from azacytidine, decitabine and revlimid.
• the present invention provides a method of treating inflammatory skin conditions such as hidradenitis suppurativa, comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from anti-TNF drugs.
• the present invention provides a method of treating inflammatory skin conditions such as atopic dermatitis, comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from IL-4/IL- 13 -targeted agents such as dupilumab.
• the present invention provides a method of treating inflammatory skin conditions such as psoriasis, comprising administering to a patient in need thereof a provided compound or a pharmaceutically acceptable salt thereof and one or more additional therapeutic agents selected from anti- IL-17 and anti-IL-23 antibodies.
• the compounds and compositions, according to the method of the present invention may be administered using any amount and any route of administration effective for treating or lessening the severity of a cancer, an autoimmune disorder, a proliferative disorder, an inflammatory disorder, a neurodegenerative or neurological disorder, schizophrenia, a bone-related disorder, liver disease, or a cardiac disorder.
• the exact amount required will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the infection, the particular agent, its mode of administration, and the like.
• Compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage.
• the expression "dosage unit form" as used herein refers to a physically discrete unit of agent appropriate for the patient to be treated.
• the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment.
• the specific effective dose level for any particular patient or organism will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed, and like factors well known in the medical arts.
• compositions of this invention can be administered to humans and other animals orally, rectally, parenterally, intracistemally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated.
• the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.
• Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs.
• the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, com, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
• the oral compositions can also
• Injectable preparations for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents.
• the sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol.
• the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.R and isotonic sodium chloride solution.
• sterile, fixed oils are conventionally employed as a solvent or suspending medium.
• any bland fixed oil can be employed including synthetic mono- or diglycerides.
• fatty acids such as oleic acid are used in the preparation of injectables.
• Injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
• the rate of compound release can be controlled.
• biodegradable polymers include poly(orthoesters) and poly(anhydrides).
• Depot injectable formulations are also prepared by entrapping the compound in liposomes or microemulsions that are compatible with body tissues.
• compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
• suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
• Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
• the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fdlers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay,
• Solid compositions of a similar type may also be employed as fdlers in soft and hard-fdled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
• the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes.
• Solid compositions of a similar type may also be employed as fdlers in soft and hard-fdled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
• the active compounds can also be in micro-encapsulated form with one or more excipients as noted above.
• the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art.
• the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch.
• Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose.
• the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner.
• buffering agents include polymeric substances and waxes.
• Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches.
• the active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required.
• Ophthalmic formulation, ear drops, and eye drops are also contemplated as being within the scope of this invention.
• the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body.
• Such dosage forms can be made by dissolving or dispensing the compound in the proper medium.
• Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel.
• the invention relates to a method of inhibiting protein kinase activity or degrading a protein kinase in a biological sample comprising the step of contacting said biological sample with a compound of this invention, or a composition comprising said compound.
• the invention relates to a method of inhibiting or degrading IRAK-1, IRAK-2, and/or IRAK-4, or a mutant thereof, activity in a biological sample comprising the step of contacting said biological sample with a compound of this invention, or a composition comprising said compound.
• biological sample includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
• Inhibition and/or degradation of a protein kinase, or a protein kinase selected from IRAK-1, IRAK-2, and/or IRAK-4, or a mutant thereof, activity in a biological sample is useful for a variety of purposes that are known to one of skill in the art. Examples of such purposes include, but are not limited to, blood transfusion, organ-transplantation, biological specimen storage, and biological assays.
• Another embodiment of the present invention relates to a method of degrading a protein kinase and/or inhibiting protein kinase activity in a patient comprising the step of administering to said patient a compound of the present invention, or a composition comprising said compound.
• the invention relates to a method of degrading and/or inhibiting one or more of IRAK-1, IRAK-2, and/or IRAK-4, or a mutant thereof, activity in a patient comprising the step of administering to said patient a compound of the present invention, or a composition comprising said compound.
• the present invention provides a method for treating a disorder mediated by one or more of IRAK- 1, IRAK-2, and/or IRAK-4, or a mutant thereof, in a patient in need thereof, comprising the step of administering to said patient a compound according to the present invention or pharmaceutically acceptable composition thereof.
• Such disorders are described in detail herein.
• additional therapeutic agents that are normally administered to treat that condition may also be present in the compositions of this invention.
• additional therapeutic agents that are normally administered to treat a particular disease, or condition are known as “appropriate for the disease, or condition, being treated.”
• a compound of the current invention may also be used to advantage in combination with other antiproliferative compounds.
• antiproliferative compounds include, but are not limited to aromatase inhibitors; antiestrogens; topoisomerase I inhibitors; topoisomerase II inhibitors; microtubule active compounds; alkylating compounds; histone deacetylase inhibitors; compounds which induce cell differentiation processes; cyclooxygenase inhibitors; MMP inhibitors; mTOR inhibitors; antineoplastic antimetabolites; platin compounds; compounds targeting/decreasing a protein or lipid kinase activity and further anti-angiogenic compounds; compounds which target, decrease or inhibit the activity of a protein or lipid phosphatase; gonadorelin agonists; anti-androgens; methionine aminopeptidase inhibitors; matrix metalloproteinase inhibitors; bisphosphonates; biological response modifiers; antiproliferative antibodies; heparanase inhibitors;
• aromatase inhibitor as used herein relates to a compound which inhibits estrogen production, for instance, the conversion of the substrates androstenedione and testosterone to estrone and estradiol, respectively.
• the term includes, but is not limited to steroids, especially atamestane, exemestane and formestane and, in particular, non-steroids, especially aminoglutethimide, roglethimide, pyridoglutethimide, trilostane, testolactone, ketokonazole, vorozole, fadrozole, anastrozole and letrozole.
• Exemestane is marketed under the trade name AromasinTM.
• Formestane is marketed under the trade name LentaronTM. Fadrozole is marketed under the trade name AfemaTM. Anastrozole is marketed under the trade name ArimidexTM. Letrozole is marketed under the trade names FemaraTM or FemarTM. Aminoglutethimide is marketed under the trade name OrimetenTM.
• a combination of the invention comprising a chemotherapeutic agent which is an aromatase inhibitor is particularly useful for the treatment of hormone receptor positive tumors, such as breast tumors.
• one or more other therapeutic agent is an mTOR inhibitor, which inhibits cell proliferation, angiogenesis and glucose uptake.
• an mTOR inhibitor is everolimus (Afmitor®, Novartis); temsirolimus (Torisel®, Pfizer); and sirolimus (Rapamune®, Pfizer).
• one or more other therapeutic agent is an aromatase inhibitor.
• an aromatase inhibitor is selected from exemestane (Aromasin®, Pfizer); anastazole (Arimidex®, AstraZeneca) and letrozole (Femara®, Novartis).
• antiestrogen as used herein relates to a compound which antagonizes the effect of estrogens at the estrogen receptor level.
• the term includes, but is not limited to tamoxifen, fulvestrant, raloxifene and raloxifene hydrochloride.
• Tamoxifen is marketed under the trade name NolvadexTM.
• Raloxifene hydrochloride is marketed under the trade name EvistaTM.
• Fulvestrant can be administered under the trade name FaslodexTM.
• a combination of the invention comprising a chemotherapeutic agent which is an antiestrogen is particularly useful for the treatment of estrogen receptor positive tumors, such as breast tumors.
• anti-androgen as used herein relates to any substance which is capable of inhibiting the biological effects of androgenic hormones and includes, but is not limited to, bicalutamide (CasodexTM).
• gonadorelin agonist as used herein includes, but is not limited to abarelix, goserelin and goserelin acetate. Goserelin can be administered under the trade name ZoladexTM.
• topoisomerase I inhibitor includes, but is not limited to topotecan, gimatecan, irinotecan, camptothecian and its analogues, 9-nitrocamptothecin and the macromolecular camptothecin conjugate PNU-166148.
• Irinotecan can be administered, e.g. in the form as it is marketed, e.g. under the trademark CamptosarTM.
• Topotecan is marketed under the trade name HycamptinTM.
• topoisomerase II inhibitor includes, but is not limited to the anthracyclines such as doxorubicin (including liposomal formulation, such as CaelyxTM), daunorubicin, epirubicin, idarubicin and nemorubicin, the anthraquinones mitoxantrone and losoxantrone, and the podophillotoxines etoposide and teniposide.
• Etoposide is marketed under the trade name EtopophosTM.
• Teniposide is marketed under the trade name VM 26-Bristol
• Doxorubicin is marketed under the trade name Acriblastin TM or AdriamycinTM.
• microtubule active agent relates to microtubule stabilizing, microtubule destabilizing compounds and microtublin polymerization inhibitors including, but not limited to taxanes, such as paclitaxel and docetaxel; vinca alkaloids, such as vinblastine or vinblastine sulfate, vincristine or vincristine sulfate, and vinorelbine; discodermolides; cochicine and epothilones and derivatives thereof.
• Paclitaxel is marketed under the trade name TaxolTM.
• Docetaxel is marketed under the trade name TaxotereTM.
• Vinblastine sulfate is marketed under the trade name Vinblastin R.PTM.
• Vincristine sulfate is marketed under the trade name FarmistinTM.
• alkylating agent includes, but is not limited to, cyclophosphamide, ifosfamide, melphalan or nitrosourea (BCNU or Gliadel). Cyclophosphamide is marketed under the trade name CyclostinTM. Ifosfamide is marketed under the trade name HoloxanTM.
• histone deacetylase inhibitors or "HDAC inhibitors” relates to compounds which inhibit the histone deacetylase and which possess antiproliferative activity. This includes, but is not limited to, suberoylanilide hydroxamic acid (SAHA).
• SAHA suberoylanilide hydroxamic acid
• antimetabolite includes, but is not limited to, 5-fluorouracil or 5-FU, capecitabine, gemcitabine, DNA demethylating compounds, such as 5-azacytidine and decitabine, methotrexate and edatrexate, and folic acid antagonists such as pemetrexed.
• Capecitabine is marketed under the trade name XelodaTM.
• Gemcitabine is marketed under the trade name GemzarTM.
• platinum compound as used herein includes, but is not limited to, carboplatin, cis- platin, cisplatinum and oxaliplatin.
• Carboplatin can be administered, e.g., in the form as it is marketed, e.g. under the trademark CarboplatTM.
• Oxaliplatin can be administered, e.g., in the form as it is marketed, e.g. under the trademark EloxatinTM.
• Bcl-2 inhibitor includes, but is not limited to compounds having inhibitory activity against B-cell lymphoma 2 protein (Bcl-2), including but not limited to ABT-199, ABT- 731, ABT-737, apogossypol, Ascenta’s pan-Bcl-2 inhibitors, curcumin (and analogs thereof), dual Bcl- 2/Bcl-xL inhibitors (Infinity Pharmaceuticals/Novartis Pharmaceuticals), Genasense (G3139), HA14-1 (and analogs thereof; see WO 2008/118802, US 2010/0197686), navitoclax (and analogs thereof, see US 7,390,799), NH-1 (Shenayng Pharmaceutical University), obatoclax (and analogs thereof, see WO 2004/106328, US 2005/0014802), S-001 (Gloria Pharmaceuticals), TW series compounds (Univ. of Michigan), and venetoclax.
• Bcl-2 B-cell lymphoma 2 protein
• the term "compounds targeting/decreasing a protein or lipid kinase activity; or a protein or lipid phosphatase activity; or further anti-angiogenic compounds” as used herein includes, but is not limited to, protein tyrosine kinase and/or serine and/or threonine kinase inhibitors or lipid kinase inhibitors, such as a) compounds targeting, decreasing or inhibiting the activity of the platelet-derived growth factor- receptors (PDGFR), such as compounds which target, decrease or inhibit the activity of PDGFR, especially compounds which inhibit the PDGF receptor, such as an N-phenyl-2-pyrimidine-amine derivative, such as imatinib, SU101, SU6668 and GFB-111; b) compounds targeting, decreasing or inhibiting the activity of the fibroblast growth factor-receptors (FGFR); c) compounds targeting, decreasing or inhibiting the activity of the insulin-like growth factor receptor I (IGF
• BCR-Abl kinase and mutants, such as compounds which target decrease or inhibit the activity of c-Abl family members and their gene fusion products, such as an N- phenyl-2-pyrimidine-amine derivative, such as imatinib or nilotinib (AMN107); PD180970; AG957; NSC 680410; PD173955 from ParkeDavis; or dasatinib (BMS-354825); j) compounds targeting, decreasing or inhibiting the activity of members of the protein kinase C (PKC) and Raf family of serine/threonine kinases, members of the MEK, SRC, JAK/pan-JAK, FAR, PDK1, PKB/Akt, Ras/MAPK, PI3K, SYK, TYK2, BTK and TEC family, and/or members of the cyclin-dependent kinase family (CDK) including staurosporine derivatives, such as midostaurin
• one or more other therapeutic agent is a growth factor antagonist, such as an antagonist of platelet-derived growth factor (PDGF), or epidermal growth factor (EGF) or its receptor (EGFR).
• PDGF platelet-derived growth factor
• EGF epidermal growth factor
• EGFR epidermal growth factor
• Approved PDGF antagonists which may be used in the present invention include olaratumab (Lartruvo®; Eli Lilly).
• Approved EGFR antagonists which may be used in the present invention include cetuximab (Erbitux®, Eli Lilly); necitumumab (Portrazza®, Eli Lilly), panitumumab (Vectibix®, Amgen); and osimertinib (targeting activated EGFR, Tagrisso®, AstraZeneca).
• PI3K inhibitor includes, but is not limited to compounds having inhibitory activity against one or more enzymes in the phosphatidylinositol-3 -kinase family, including, but not limited to RI3Ka, RI3Kg, RBKd, RI3Kb, PI3K-C2a, RI3K-02b, PI3K-C2y, Vps34, pll0-a, r110-b, r110-g, r110-d, r85-a, r85-b, r55-g, r 150, plOl, and r87.
• RI3K inhibitors useful in this invention include but are not limited to ATU-027, SF-1126, DS-7423, PBI-05204, GSK-2126458, ZSTK- 474, buparlisib, pictrelisib, PF-4691502, BYL-719, dactolisib, XL-147, XL-765, and idelalisib.
• BTK inhibitor includes, but is not limited to compounds having inhibitory activity against Bruton’s Tyrosine Kinase (BTK), including, but not limited to AVL-292 and ibrutinib.
• SYK inhibitor includes, but is not limited to compounds having inhibitory activity against spleen tyrosine kinase (SYK), including but not limited to PRT-062070, R-343, R-333, Excellair, PRT-062607, and fostamatinib
• BTK inhibitory compounds, and conditions treatable by such compounds in combination with compounds of this invention can be found in WO 2008/039218, US 2008/0108636 and WO 2011/090760, US 2010/0249092, the entirety of each of which is herein incorporated by reference.
• SYK inhibitory compounds, and conditions treatable by such compounds in combination with compounds of this invention can be found in WO 2003/063794, US 2004/0029902, WO 2005/007623, US 2005/0075306, and WO 2006/078846, US 2006/0211657, the entirety of each of which is herein incorporated by reference.
• PI3K inhibitory compounds and conditions treatable by such compounds in combination with compounds of this invention can be found in WO 2004/019973, US 2004/0106569, WO 2004/089925, US 2004/0242631, US 8,138,347, WO 2002/088112, US 2004/0116421, WO 2007/084786, US 2010/0249126, WO 2007/129161, US 2008/0076768, WO 2006/122806, US 2008/0194579, WO 2005/113554, US 2008/0275067, and WO 2007/044729, US 2010/0087440, the entirety of each of which is herein incorporated by reference.
• JAK inhibitory compounds and conditions treatable by such compounds in combination with compounds of this invention can be found in WO 2009/114512, US 2009/0233903, WO 2008/109943, US 2010/0197671, WO 2007/053452, US 2007/0191405, WO 2001/0142246, US 2001/0053782, and WO 2007/070514, US 2007/0135461, the entirety of each of which is herein incorporated by reference.
• Further anti-angiogenic compounds include compounds having another mechanism for their activity, e.g. unrelated to protein or lipid kinase inhibition e.g. thalidomide (ThalomidTM) and TNP-470.
• proteasome inhibitors useful for use in combination with compounds of the invention include, but are not limited to bortezomib, disulfiram, epigallocatechin-3-gallate (EGCG), salinosporamide A, carfdzomib, ONX-0912, CEP-18770, and MLN9708.
• Compounds which target, decrease or inhibit the activity of a protein or lipid phosphatase are e.g. inhibitors of phosphatase 1, phosphatase 2A, or CDC25, such as okadaic acid or a derivative thereof.
• Compounds which induce cell differentiation processes include, but are not limited to, retinoic acid, a- y- or d- tocopherol or a- y- or d-tocotrienol.
• cyclooxygenase inhibitor as used herein includes, but is not limited to, Cox-2 inhibitors, 5 -alkyl substituted 2-arylaminophenylacetic acid and derivatives, such as celecoxib (CelebrexTM), rofecoxib (VioxxTM), etoricoxib, valdecoxib or a 5 -alkyl-2- arylaminophenylacetic acid, such as 5-methyl-2-(2'-chloro-6'-fluoroanilino)phenyl acetic acid, lumiracoxib.
• Cox-2 inhibitors such as celecoxib (CelebrexTM), rofecoxib (VioxxTM), etoricoxib, valdecoxib or a 5 -alkyl-2- arylaminophenylacetic acid, such as 5-methyl-2-(2'-chloro-6'-fluoroanilino)phenyl acetic acid, lumiracoxib.
• bisphosphonates includes, but is not limited to, etridonic, clodronic, tiludronic, pamidronic, alendronic, ibandronic, risedronic and zoledronic acid.
• Etridonic acid is marketed under the trade name DidronelTM.
• Clodronic acid is marketed under the trade name BonefosTM.
• Tiludronic acid is marketed under the trade name SkelidTM.
• Pamidronic acid is marketed under the trade name ArediaTM.
• Alendronic acid is marketed under the trade name FosamaxTM.
• Ibandronic acid is marketed under the trade name BondranatTM.
• Risedronic acid is marketed under the trade name ActonelTM.
• Zoledronic acid is marketed under the trade name ZometaTM.
• mTOR inhibitors relates to compounds which inhibit the mammalian target of rapamycin (mTOR) and which possess antiproliferative activity such as sirolimus (Rapamune®), everolimus (CerticanTM), CCI-779 and ABT578.
• heparanase inhibitor refers to compounds which target, decrease or inhibit heparin sulfate degradation.
• the term includes, but is not limited to, PI-88.
• biological response modifier refers to a lymphokine or interferons.
• inhibitor of Ras oncogenic isoforms such as H-Ras, K-Ras, or N-Ras
• a "famesyl transferase inhibitor” such as L-744832, DK8G557 or R115777 (ZamestraTM).
• telomerase inhibitor refers to compounds which target, decrease or inhibit the activity of telomerase. Compounds which target, decrease or inhibit the activity of telomerase are especially compounds which inhibit the telomerase receptor, such as telomestatin.
• methionine aminopeptidase inhibitor refers to compounds which target, decrease or inhibit the activity of methionine aminopeptidase.
• Compounds which target, decrease or inhibit the activity of methionine aminopeptidase include, but are not limited to, bengamide or a derivative thereof.
• proteasome inhibitor refers to compounds which target, decrease or inhibit the activity of the proteasome.
• Compounds which target, decrease or inhibit the activity of the proteasome include, but are not limited to, Bortezomib (VelcadeTM), ); carfilzomib (Kyprolis®, Amgen); and ixazomib (Ninlaro®, Takeda), and MLN 341.
• matrix metalloproteinase inhibitor or (“MMP” inhibitor) as used herein includes, but is not limited to, collagen peptidomimetic and nonpeptidomimetic inhibitors, tetracycline derivatives, e.g. hydroxamate peptidomimetic inhibitor batimastat and its orally bioavailable analogue marimastat (BB- 2516), prinomastat (AG3340), metastat (NSC 683551) BMS-279251 , BAY 12-9566, TAA211 , MMI270B or AAJ996.
• MMP matrix metalloproteinase inhibitor
• FMS-like tyrosine kinase inhibitors which are compounds targeting, decreasing or inhibiting the activity of FMS-like tyrosine kinase receptors (Flt-3R); interferon, I-b-D- arabinofuransylcytosine (ara-c) and bisulfan; and ALK inhibitors, which are compounds which target, decrease or inhibit anaplastic lymphoma kinase.
• FMS-like tyrosine kinase receptors are especially compounds, proteins or antibodies which inhibit members of the Flt-3R receptor kinase family, such as PKC412, midostaurin, a staurosporine derivative, SU11248 and MLN518.
• HSP90 inhibitors includes, but is not limited to, compounds targeting, decreasing or inhibiting the intrinsic ATPase activity of HSP90; degrading, targeting, decreasing or inhibiting the HSP90 client proteins via the ubiquitin proteosome pathway.
• Compounds targeting, decreasing or inhibiting the intrinsic ATPase activity of HSP90 are especially compounds, proteins or antibodies which inhibit the ATPase activity ofHSP90, such as 17-allylamino,17-demethoxygeldanamycin (17AAG), a geldanamycin derivative; other geldanamycin related compounds; radicicol and HDAC inhibitors.
• antiproliferative antibodies includes, but is not limited to, trastuzumab (HerceptinTM), Trastuzumab-DMl, erbitux, bevacizumab (AvastinTM), rituximab (Rituxan ® ), PR064553 (anti-CD40) and 2C4 Antibody.
• trastuzumab HerceptinTM
• Trastuzumab-DMl erbitux
• bevacizumab AvastinTM
• rituximab Rasteran ®
• PR064553 anti-CD40
• compounds of the current invention can be used in combination with standard leukemia therapies, especially in combination with therapies used for the treatment of AML.
• compounds of the current invention can be administered in combination with, for example, famesyl transferase inhibitors and/or other drugs useful for the treatment of AML, such as Daunorubicin, Adriamycin, Ara-C, VP- 16, Teniposide, Mitoxantrone, Idarubicin, Carboplatinum and PKC412.
• HDAC histone deacetylase
• SAHA suberoylanilide hydroxamic acid
• HDAC inhibitors include MS275, SAHA, FK228 (formerly FR901228), Trichostatin A and compounds disclosed in US 6,552,065 including, but not limited to, N-hydroxy-3-[4-[[[2-(2-methyl-lH-indol-3-yl)- ethyl]- amino]methyl]phenyl]-2E-2-propenamide, or a pharmaceutically acceptable salt thereof and N- hydroxy-3-[4-[(2-hydroxyethyl) ⁇ 2-(lH-indol-3-yl)ethyl]-amino]methyl]phenyl]-2E-2- propenamide, or a pharmaceutically acceptable salt thereof, especially the lactate salt.
• Somatostatin receptor antagonists as used herein refer to compounds which target, treat or inhibit the somatostatin receptor such as octreotide, and SOM230.
• Tumor cell damaging approaches refer to approaches such as ionizing radiation.
• the term "ionizing radiation” referred to above and hereinafter means ionizing radiation that occurs as either electromagnetic rays (such as X-rays and gamma rays) or particles (such as alpha and beta particles). Ionizing radiation is provided in, but not limited to, radiation therapy and is known in the art. See Heilman, Principles of Radiation Therapy, Cancer, in Principles and Practice of Oncology, Devita et ah, Eds., 4 th Edition, Vol. 1 , pp. 248-275 (1993).
• EDG binders and ribonucleotide reductase inhibitors.
• EDG binders refers to a class of immunosuppressants that modulates lymphocyte recirculation, such as FTY720.
• ribonucleotide reductase inhibitors refers to pyrimidine or purine nucleoside analogs including, but not limited to, fludarabine and/or cytosine arabinoside (ara-C), 6-thioguanine, 5- fluorouracil, cladribine, 6-mercaptopurine (especially in combination with ara-C against ALL) and/or pentostatin.
• Ribonucleotide reductase inhibitors are especially hydroxyurea or 2 -hydroxy- lH-isoindole-1 ,3-dione derivatives.
• VEGF vascular endothelial growth factor
• l-(4-chloroanilino)-4-(4-pyridylmethyl)phthalazine or a pharmaceutically acceptable salt thereof l-(4-chloroanilino)-4-(4-pyridylmethyl)phthalazine succinate
• AngiostatinTM EndostatinTM
• anthranilic acid amides ZD4190; ZD6474; SU5416; SU6668
• bevacizumab or anti-VEGF antibodies or anti-VEGF receptor antibodies, such as rhuMAb and RHUFab
• VEGF aptamer such as Macugon
• FLT-4 inhibitors, FLT-3 inhibitors VEGFR-2 IgGI antibody
• Angiozyme RI 4610)
• Bevacizumab AvastinTM
• Photodynamic therapy refers to therapy which uses certain chemicals known as photosensitizing compounds to treat or prevent cancers.
• Examples of photodynamic therapy include treatment with compounds, such as VisudyneTM and porfimer sodium.
• Angiostatic steroids refers to compounds which block or inhibit angiogenesis, such as, e.g., anecortave, triamcinolone, hydrocortisone, 11-a-epihydrocotisol, cortexolone, 17a- hydroxyprogesterone, corticosterone, desoxycorticosterone, testosterone, estrone and dexamethasone.
• angiogenesis such as, e.g., anecortave, triamcinolone, hydrocortisone, 11-a-epihydrocotisol, cortexolone, 17a- hydroxyprogesterone, corticosterone, desoxycorticosterone, testosterone, estrone and dexamethasone.
• Implants containing corticosteroids refers to compounds, such as fluocinolone and dexamethasone.
• chemotherapeutic compounds include, but are not limited to, plant alkaloids, hormonal compounds and antagonists; biological response modifiers, preferably lymphokines or interferons; antisense oligonucleotides or oligonucleotide derivatives; shRNA or siRNA; or miscellaneous compounds or compounds with other or unknown mechanism of action.
• the compounds of the invention are also useful as co-therapeutic compounds for use in combination with other drug substances such as anti-inflammatory, bronchodilatory or antihistamine drug substances, particularly in the treatment of obstructive or inflammatory airways diseases such as those mentioned hereinbefore, for example as potentiators of therapeutic activity of such drugs or as a means of reducing required dosaging or potential side effects of such drugs.
• a compound of the invention may be mixed with the other drug substance in a fixed pharmaceutical composition or it may be administered separately, before, simultaneously with or after the other drug substance.
• the invention includes a combination of a compound of the invention as hereinbefore described with an anti-inflammatory, bronchodilatory, antihistamine or anti-tussive drug substance, said compound of the invention and said drug substance being in the same or different pharmaceutical composition.
• Suitable anti-inflammatory drugs include steroids, in particular glucocorticosteroids such as budesonide, beclamethasone dipropionate, fluticasone propionate, ciclesonide or mometasone furoate; non steroidal glucocorticoid receptor agonists; LTB4 antagonists such LY293111, CGS025019C, CP-195543, SC-53228, BIIL 284, ONO 4057, SB 209247; LTD4 antagonists such as montelukast and zafirlukast; PDE4 inhibitors such cilomilast (Ariflo® GlaxoSmithKline), Roflumilast (Byk Gulden),V-11294A (Napp), BAY19-8004 (Bayer), SCH-351591 (Schering- Plough), Arofylline (Almirall Prodesfarma), PD189659 / PD168787 (Parke-D
• steroids in
• Suitable bronchodilatory drugs include anticholinergic or antimuscarinic compounds, in particular ipratropium bromide, oxitropium bromide, tiotropium salts and CHF 4226 (Chiesi), and glycopyrrolate.
• Suitable antihistamine drug substances include cetirizine hydrochloride, acetaminophen, clemastine fumarate, promethazine, loratidine, desloratidine, diphenhydramine and fexofenadine hydrochloride, activastine, astemizole, azelastine, ebastine, epinastine, mizolastine and tefenadine.
• Other useful combinations of compounds of the invention with anti-inflammatory drugs are those with antagonists of chemokine receptors, e.g.
• TAK-770 Trigger-770
• a compound of the current invention may also be used in combination with known therapeutic processes, for example, the administration of hormones or radiation.
• a provided compound is used as a radiosensitizer, especially for the treatment of tumors which exhibit poor sensitivity to radiotherapy.
• a compound of the current invention can be administered alone or in combination with one or more other therapeutic compounds, possible combination therapy taking the form of fixed combinations or the administration of a compound of the invention and one or more other therapeutic compounds being staggered or given independently of one another, or the combined administration of fixed combinations and one or more other therapeutic compounds.
• a compound of the current invention can besides or in addition be administered especially for tumor therapy in combination with chemotherapy, radiotherapy, immunotherapy, phototherapy, surgical intervention, or a combination of these. Long-term therapy is equally possible as is adjuvant therapy in the context of other treatment strategies, as described above . Other possible treatments are therapy to maintain the patient's status after tumor regression, or even chemopreventive therapy, for example in patients at risk.
• Those additional agents may be administered separately from an inventive compound- containing composition, as part of a multiple dosage regimen. Alternatively, those agents may be part of a single dosage form, mixed together with a compound of this invention in a single composition. If administered as part of a multiple dosage regime, the two active agents may be submitted simultaneously, sequentially or within a period of time from one another normally within five hours from one another.
• the term “combination,” “combined,” and related terms refers to the simultaneous or sequential administration of therapeutic agents in accordance with this invention. For example, a compound of the present invention may be administered with another therapeutic agent simultaneously or sequentially in separate unit dosage forms or together in a single unit dosage form.
• the present invention provides a single unit dosage form comprising a compound of the current invention, an additional therapeutic agent, and a pharmaceutically acceptable carrier, adjuvant, or vehicle.
• the amount of both an inventive compound and additional therapeutic agent in those compositions which comprise an additional therapeutic agent as described above, that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration.
• compositions of this invention should be formulated so that a dosage of between 0.01 - 100 mg/kg body weight/day of an inventive compound can be administered.
• that additional therapeutic agent and the compound of this invention may act synergistically. Therefore, the amount of additional therapeutic agent in such compositions will be less than that required in a monotherapy utilizing only that therapeutic agent. In such compositions a dosage of between 0.01 - 1,000 pg/kg body weight/day of the additional therapeutic agent can be administered.
• the amount of one or more other therapeutic agent present in the compositions of this invention may be no more than the amount that would normally be administered in a composition comprising that therapeutic agent as the only active agent.
• the amount of one or more other therapeutic agent in the presently disclosed compositions will range from about 50% to 100% of the amount normally present in a composition comprising that agent as the only therapeutically active agent.
• one or more other therapeutic agent is administered at a dosage of about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, or about 95% of the amount normally administered for that agent.
• the phrase “normally administered” means the amount an FDA approved therapeutic agent is approved for dosing per the FDA label insert.
• the compounds of this invention, or pharmaceutical compositions thereof, may also be incorporated into compositions for coating an implantable medical device, such as prostheses, artificial valves, vascular grafts, stents and catheters.
• an implantable medical device such as prostheses, artificial valves, vascular grafts, stents and catheters.
• Vascular stents for example, have been used to overcome restenosis (re-narrowing of the vessel wall after injury) .
• patients using stents or other implantable devices risk clot formation or platelet activation. These unwanted effects may be prevented or mitigated by pre-coating the device with a pharmaceutically acceptable composition comprising a kinase inhibitor.
• Implantable devices coated with a compound of this invention are another embodiment of the present invention.
• one or more other therapeutic agent is an immuno-oncology agent.
• an immuno-oncology agent refers to an agent which is effective to enhance, stimulate, and/or up-regulate immune responses in a subject.
• the administration of an immuno-oncology agent with a compound of the invention has a synergic effect in treating a cancer.
• An immuno-oncology agent can be, for example, a small molecule drug, an antibody, or a biologic or small molecule.
• biologic immuno-oncology agents include, but are not limited to, cancer vaccines, antibodies, and cytokines.
• an antibody is a monoclonal antibody.
• a monoclonal antibody is humanized or human.
• an immuno-oncology agent is (i) an agonist of a stimulatory (including a co-stimulatory) receptor or (ii) an antagonist of an inhibitory (including a co-inhibitory) signal on T cells, both of which result in amplifying antigen-specific T cell responses.
• Certain of the stimulatory and inhibitory molecules are members of the immunoglobulin super family (IgSF).
• IgSF immunoglobulin super family
• B7 family which includes B7-1, B7-2, B7-H1 (PD-L1), B7-DC (PD-L2), B7-H2 (ICOS-L), B7-H3, B7-H4, B7-H5 (VISTA), and B7-H6.
• TNF family of molecules that bind to cognate TNF receptor family members which includes CD40 and CD40F, OX-40, OX-40F, CD70, CD27F, CD30, CD30F, 4-1BBF, CD137 (4-1BB), TRAIF/Apo2-F, TRAIFR1/DR4, TRAIFR2/DR5, TRAIFR3, TRAIFR4, OPG, RANK, RANKE, TWEAKR/Fnl4, TWEAK, BAFFR, EDAR, XEDAR, TACI, APRIF, BCMA, LT R, FIGHT, DcR3, HVEM, VEGI/TF1A, TRAMP/DR3, EDAR, EDA1, XEDAR, EDA2, TNFR1, Lymphotoxin a/TNEb, TNFR2, TNFa, EGbI4.
• TNF family of molecules that bind to cognate TNF receptor family members which includes CD40 and CD40F, OX-40, OX-40F, CD70, CD27
• an immuno-oncology agent is a cytokine that inhibits T cell activation (e.g., IL-6, IL-10, TGF-b, VEGF, and other immunosuppressive cytokines) or a cytokine that stimulates T cell activation, for stimulating an immune response.
• T cell activation e.g., IL-6, IL-10, TGF-b, VEGF, and other immunosuppressive cytokines
• an immuno-oncology agent is: (i) an antagonist of a protein that inhibits T cell activation (e.g., immune checkpoint inhibitors) such as CTLA-4, PD-1, PD- Ll, PD-L2, LAG-3, TIM-3, Galectin 9, CEACAM-1, BTLA, CD69, Galectin-1, TIGIT, CD113, GPR56, VISTA, 2B4, CD48, GARP, PD 1H, LAIR1 , TIM- 1 , and TIM-4; or (ii) an agonist of a protein that stimulates T cell activation such as B7-1, B7-2, CD28, 4-1BB (CD137), 4-1BBL, ICOS, ICOS-L, 0X40, OX40L, GITR, GITRL, CD70, CD27, CD40, DR3
• an antagonist of a protein that inhibits T cell activation e.g., immune checkpoint inhibitors
• CTLA-4 e.g., immune
• an immuno-oncology agent is an antagonist of inhibitory receptors on NK cells or an agonists of activating receptors on NK cells.
• an immuno-oncology agent is an antagonists of KIR, such as lirilumab.
• an immuno-oncology agent is an agent that inhibits or depletes macrophages or monocytes, including but not limited to CSF-1R antagonists such as CSF-1R antagonist antibodies including RG7155 (WO 2011/070024, US 2011/0165156, WO 2011/0107553, US 2012/0329997, WO 2011/131407, US 2013/0005949, WO 2013/087699, US 2014/0336363, WO 2013/119716, WO 2013/132044, US 2014/0079706) or FPA-008 (WO 2011/140249, US 2011/0274683; WO 2013/169264; WO 2014/036357, US 2014/0079699).
• CSF-1R antagonists such as CSF-1R antagonist antibodies including RG7155 (WO 2011/070024, US 2011/0165156, WO 2011/0107553, US 2012/0329997, WO 2011/131407, US 2013/0005949, WO 2013/087699, US 2014
• an immuno-oncology agent is selected from agonistic agents that ligate positive costimulatory receptors, blocking agents that attenuate signaling through inhibitory receptors, antagonists, and one or more agents that increase systemically the frequency of anti-tumor T cells, agents that overcome distinct immune suppressive pathways within the tumor microenvironment (e.g., block inhibitory receptor engagement (e.g., PD-Ll/PD-1 interactions), deplete or inhibit Tregs (e.g., using an anti- CD25 monoclonal antibody (e.g., daclizumab) or by ex vivo anti-CD25 bead depletion), inhibit metabolic enzymes such as IDO, or reverse/prevent T cell energy or exhaustion) and agents that trigger innate immune activation and/or inflammation at tumor sites.
• block inhibitory receptor engagement e.g., PD-Ll/PD-1 interactions
• Tregs e.g., using an anti- CD25 monoclonal antibody (e.g., daclizumab) or
• an immuno-oncology agent is a CTLA-4 antagonist.
• a CTLA-4 antagonist is an antagonistic CTLA-4 antibody.
• an antagonistic CTLA-4 antibody is YERVOY (ipilimumab) or tremelimumab.
• an immuno-oncology agent is a PD-1 antagonist.
• a PD-1 antagonist is administered by infusion.
• an immuno-oncology agent is an antibody or an antigen-binding portion thereof that binds specifically to a Programmed Death- 1 (PD-1) receptor and inhibits PD-1 activity.
• a PD-1 antagonist is an antagonistic PD-1 antibody.
• an antagonistic PD-1 antibody is OPDIVO (nivolumab), KEYTRUDA (pembrolizumab), or MEDI-0680 (AMP-514; WO2012/145493).
• an immuno-oncology agent may be pidilizumab (CT-011).
• an immuno-oncology agent is a recombinant protein composed of the extracellular domain of PD-L2 (B7-DC) fused to the Fc portion of IgGl, called AMP-224.
• an immuno-oncology agent is a PD-L1 antagonist.
• a PD-L1 antagonist is an antagonistic PD-L1 antibody.
• a PD-L1 antibody is MPDL3280A (RG7446; WO 2010/077634, US 2010/0203056), durvalumab (MEDI4736), BMS-936559 (WO 2007/005874, US 2009/0055944), and MSB0010718C (WO 2013/079174, US 2014/0341917).
• an immuno-oncology agent is a LAG-3 antagonist.
• a LAG-3 antagonist is an antagonistic LAG-3 antibody.
• a LAG3 antibody is BMS-986016 (WO 2010/019570, US 2010/0150892, WO 2014/008218, US 2014/0093511), or IMP-731 or IMP-321 (WO 2008/132601, US 2010/0233183, WO 2009/044273, US 2011/0008331).
• an immuno-oncology agent is a CD137 (4-1BB) agonist.
• a CD 137 (4- IBB) agonist is an agonistic CD 137 antibody.
• a CD 137 antibody is urelumab or PF-05082566 (W012/32433).
• an immuno-oncology agent is a GITR agonist.
• a GITR agonist is an agonistic GITR antibody.
• a GITR antibody is BMS-986153, BMS-986156, TRX-518 (WO 2006/105021, US 2007/0098719, WO 2009/009116, US 2009/0136494), or MK-4166 (WO 2011/028683, US 2012/0189639).
• an immuno-oncology agent is an indoleamine (2,3)-dioxygenase (IDO) antagonist.
• IDO antagonist is selected from epacadostat (INCB024360, Incyte); indoximod (NLG-8189, NewLink Genetics Corporation); capmanitib (INC280, Novartis); GDC-0919 (Genentech/Roche); PF-06840003 (Pfizer); BMS:F001287 (Bristol-Myers Squibb); Phy906/KD108 (Phytoceutica); an enzyme that breaks down kynurenine (Kynase, Kyn Therapeutics); and NLG-919 (WO 2009/073620, US 2011/053941, WO 2009/132238, US 2011/136796, WO 2011/056652, US 2012/277217, WO 2012/142237, US 2014/066625).
• an immuno-oncology agent is an 0X40 agonist.
• an 0X40 agonist is an agonistic 0X40 antibody.
• an 0X40 antibody is MEDI-6383 or MEDI-6469.
• an immuno-oncology agent is an OX40L antagonist.
• an OX40L antagonist is an antagonistic 0X40 antibody.
• an OX40L antagonist is RG-7888 (WO 2006/029879, US 7,501,496).
• an immuno-oncology agent is a CD40 agonist.
• a CD40 agonist is an agonistic CD40 antibody.
• an immuno-oncology agent is a CD40 antagonist.
• a CD40 antagonist is an antagonistic CD40 antibody.
• a CD40 antibody is lucatumumab or dacetuzumab.
• an immuno-oncology agent is a CD27 agonist.
• a CD27 agonist is an agonistic CD27 antibody.
• a CD27 antibody is varlilumab.
• an immuno-oncology agent is MGA271 (to B7H3) (WO 2011/109400, US 2013/0149236).
• an immuno-oncology agent is abagovomab, adecatumumab, afutuzumab, alemtuzumab, anatumomab mafenatox, apolizumab, atezolimab, avelumab, blinatumomab, BMS-936559, catumaxomab, durvalumab, epacadostat, epratuzumab, indoximod, inotuzumab ozogamicin, intelumumab, ipilimumab, isatuximab, lambrolizumab, MED 14736, MPDL3280A, nivolumab, obinutuzumab, ocaratuzumab, ofatumumab, olatatumab, pembrolizumab, pidilizumab, rituxima
• an immuno-oncology agent is an immunostimulatory agent.
• antibodies blocking the PD-1 and PD-L1 inhibitory axis can unleash activated tumor-reactive T cells and have been shown in clinical trials to induce durable anti-tumor responses in increasing numbers of tumor histologies, including some tumor types that conventionally have not been considered immunotherapy sensitive. See, e.g., Okazaki, T. el al. (2013) Nat. Immunol. 14, 1212-1218; Zou el al. (2016) Sci. Transl. Med. 8.
• the anti-PD-1 antibody nivolumab (Opdivo ® , Bristol-Myers Squibb, also known as ONO-4538, MDX1106 and BMS-936558), has shown potential to improve the overall survival in patients with RCC who had experienced disease progression during or after prior anti -angiogenic therapy.
• the immunomodulatory therapeutic specifically induces apoptosis of tumor cells.
• Approved immunomodulatory therapeutics which may be used in the present invention include pomalidomide (Pomalyst®, Celgene); lenalidomide (Revlimid®, Celgene); ingenol mebutate (Picato®, LEO Pharma).
• an immuno-oncology agent is a cancer vaccine.
• the cancer vaccine is selected from sipuleucel-T (Provenge®, Dendreon/Valeant Pharmaceuticals), which has been approved for treatment of asymptomatic, or minimally symptomatic metastatic castrate-resistant (hormone-refractory) prostate cancer; and talimogene laherparepvec (Imlygic®, BioVex/Amgen, previously known as T-VEC), a genetically modified oncolytic viral therapy approved for treatment of unresectable cutaneous, subcutaneous and nodal lesions in melanoma.
• an immuno- oncology agent is selected from an oncolytic viral therapy such as pexastimogene devacirepvec (PexaVec/JX-594, SillaJen/formerly Jennerex Biotherapeutics), a thymidine kinase- (TK-) deficient vaccinia virus engineered to express GM-CSF, for hepatocellular carcinoma (NCT02562755) and melanoma (NCT00429312); pelareorep (Reolysin®, Oncolytics Biotech), a variant of respiratory enteric orphan virus (reovirus) which does not replicate in cells that are not RAS-activated, in numerous cancers, including colorectal cancer (NCT01622543); prostate cancer (NCT01619813); head and neck squamous cell cancer (NCT01166542); pancreatic adenocarcinoma (NCT00998322); and non-small cell lung cancer (NSCLC) (
• an immuno-oncology agent is selected from JX-929 (SillaJen/formerly Jennerex Biotherapeutics), a TK- and vaccinia growth factor-deficient vaccinia virus engineered to express cytosine deaminase, which is able to convert the prodrug 5-fluorocytosine to the cytotoxic drug 5- fluorouracil; TG01 and TG02 (Targovax/formerly Oncos), peptide-based immunotherapy agents targeted for difficult-to-treat RAS mutations; and TILT- 123 (TILT Biotherapeutics), an engineered adenovirus designated: Ad5/3-E2F-delta24-hTNFa-IRES-hIL20; and VSV-GP (ViraTherapeutics) a vesicular stomatitis virus (VSV) engineered to express the glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV), which can
• an immuno-oncology agent is a T-cell engineered to express a chimeric antigen receptor, or CAR.
• the T-cells engineered to express such chimeric antigen receptor are referred to as a CAR-T cells.
• CARs have been constructed that consist of binding domains, which may be derived from natural ligands, single chain variable fragments (scFv) derived from monoclonal antibodies specific for cell-surface antigens, fused to endodomains that are the functional end of the T-cell receptor (TCR), such as the CD3-zeta signaling domain from TCRs, which is capable of generating an activation signal in T lymphocytes.
• TCR T-cell receptor
• the CAR-T cell is one of those described in U.S.
• Patent 8,906,682 the entirety of each of which is herein incorporated by reference, which discloses CAR-T cells engineered to comprise an extracellular domain having an antigen binding domain (such as a domain that binds to CD 19), fused to an intracellular signaling domain of the T cell antigen receptor complex zeta chain (such as CD3 zeta).
• an antigen binding domain such as a domain that binds to CD 19
• CD3 zeta intracellular signaling domain of the T cell antigen receptor complex zeta chain
• the CAR When expressed in the T cell, the CAR is able to redirect antigen recognition based on the antigen binding specificity. In the case of CD 19, the antigen is expressed on malignant B cells.
• CD 19 the antigen binding specificity
• CD 19 the antigen is expressed on malignant B cells.
• Over 200 clinical trials are currently in progress employing CAR-T in a wide range of indications. [https://clinicaltrials.gov/ct2/results
• an immunostimulatory agent is an activator of retinoic acid receptor- related orphan receptor g (RORyt).
• RORyt is a transcription factor with key roles in the differentiation and maintenance of Type 17 effector subsets of CD4+ (Thl7) and CD8+ (Tcl7) T cells, as well as the differentiation of IL-17 expressing innate immune cell subpopulations such as NK cells.
• an activator of RORyt is LYC-55716 (Lycera), which is currently being evaluated in clinical trials for the treatment of solid tumors (NCT02929862).
• an immunostimulatory agent is an agonist or activator of a toll-like receptor (TLR).
• TLR toll-like receptor
• Suitable activators of TLRs include an agonist or activator of TLR9 such as SD-101 (Dynavax).
• SD-101 is an immunostimulatory CpG which is being studied for B-cell, follicular and other lymphomas (NCT02254772).
• Agonists or activators of TLR8 which may be used in the present invention include motolimod (VTX-2337, VentiRx Pharmaceuticals) which is being studied for squamous cell cancer of the head and neck (NCT02124850) and ovarian cancer (NCT02431559).
• immuno-oncology agents that may be used in the present invention include urelumab (BMS-663513, Bristol-Myers Squibb), an anti-CD137 monoclonal antibody; varlilumab (CDX-1127, Celldex Therapeutics), an anti-CD27 monoclonal antibody; BMS-986178 (Bristol-Myers Squibb), an anti- 0X40 monoclonal antibody; lirilumab (IPH2102/BMS-986015, Innate Pharma, Bristol-Myers Squibb), an anti-KIR monoclonal antibody; monalizumab (IPH2201, Innate Pharma, AstraZeneca) an anti-NKG2A monoclonal antibody; andecaliximab (GS-5745, Gilead Sciences), an anti-MMP9 antibody; MK-4166 (Merck & Co.), an anti-GITR monoclonal antibody.
• BMS-663513 Bristol-Myers Squib
• an immunostimulatory agent is selected from elotuzumab, mifamurtide, an agonist or activator of a toll -like receptor, and an activator of RORyt.
• an immunostimulatory therapeutic is recombinant human interleukin 15 (rhIL-15).
• rhIL-15 has been tested in the clinic as a therapy for melanoma and renal cell carcinoma (NCT01021059 and NCT01369888) and leukemias (NCT02689453).
• an immunostimulatory agent is recombinant human interleukin 12 (rhIL-12).
• an IL-15 based immunotherapeutic is heterodimeric IL-15 (hetIL-15, Novartis/Admune), a fusion complex composed of a synthetic form of endogenous IL-15 complexed to the soluble IL-15 binding protein IL-15 receptor alpha chain (IL15:sIL-15RA), which has been tested in Phase 1 clinical trials for melanoma, renal cell carcinoma, non-small cell lung cancer and head and neck squamous cell carcinoma (NCT02452268).
• a recombinant human interleukin 12 (rhIL-12) is NM-IL-12 (Neumedicines, Inc.), NCT02544724, or NCT02542124.
• an immuno-oncology agent is selected from those descripted in Jerry L. Adams ET. AL., “Big opportunities for small molecules in immuno-oncology,” Cancer Therapy 2015, Vol. 14, pages 603-622, the content of which is incorporated herein by reference in its entirety.
• an immuno-oncology agent is selected from the examples described in Table 1 of Jerry L. Adams ET. AL.
• an immuno-oncology agent is a small molecule targeting an immuno-oncology target selected from those listed in Table 2 of Jerry L. Adams ET. AL.
• an immuno-oncology agent is a small molecule agent selected from those listed in Table 2 of Jerry L. Adams ET. AL.
• an immuno-oncology agent is selected from the small molecule immuno-oncology agents described in Peter L. Toogood, “Small molecule immuno-oncology therapeutic agents,” Bioorganic & Medicinal Chemistry Letters 2018, Vol. 28, pages 319-329, the content of which is incorporated herein by reference in its entirety.
• an immuno-oncology agent is an agent targeting the pathways as described in Peter L. Toogood.
• an immuno-oncology agent is selected from those described in Sandra L. Ross et ak, “Bispecific T cell engager (BiTE® ) antibody constructs can mediate bystander tumor cell killing”, PLoS ONE 12(8): eO 183390, the content of which is incorporated herein by reference in its entirety.
• an immuno-oncology agent is a bispecific T cell engager (BiTE®) antibody construct.
• a bispecific T cell engager (BiTE®) antibody construct is a CD19/CD3 bispecific antibody construct.
• a bispecific T cell engager (BiTE®) antibody construct is an EGFR/CD3 bispecific antibody construct.
• a bispecific T cell engager (BiTE®) antibody construct activates T cells.
• a bispecific T cell engager (BiTE®) antibody construct activates T cells, which release cytokines inducing upregulation of intercellular adhesion molecule 1 (ICAM-1) and FAS on bystander cells.
• a bispecific T cell engager (BiTE®) antibody construct activates T cells which result in induced bystander cell lysis.
• the bystander cells are in solid tumors.
• the bystander cells being lysed are in proximity to the BiTE®-activated T cells.
• the bystander cells comprises tumor-associated antigen (TAA) negative cancer cells.
• TAA tumor-associated antigen
• an immuno-oncology agent is an antibody which blocks the PD-L1/PD1 axis and/or CTLA4.
• an immuno-oncology agent is an ex- vivo expanded tumor-infdtrating T cell.
• an immuno-oncology agent is a bispecific antibody construct or chimeric antigen receptors (CARs) that directly connect T cells with tumor-associated surface antigens (TAAs).
• an immuno-oncology agent is an immune checkpoint inhibitor as described herein.
• checkpoint inhibitor as used herein relates to agents useful in preventing cancer cells from avoiding the immune system of the patient.
• T-cell exhaustion One of the major mechanisms of anti-tumor immunity subversion is known as “T-cell exhaustion,” which results from chronic exposure to antigens that has led to up-regulation of inhibitory receptors. These inhibitory receptors serve as immune checkpoints in order to prevent uncontrolled immune reactions.
• PD- 1 and co-inhibitory receptors such as cytotoxic T-lymphocyte antigen 4 (CTLA-4, B and T Lymphocyte Attenuator (BTLA; CD272), T cell Immunoglobulin and Mucin domain-3 (Tim-3), Lymphocyte Activation Gene-3 (Lag-3; CD223), and others are often referred to as a checkpoint regulators. They act as molecular “gatekeepers” that allow extracellular information to dictate whether cell cycle progression and other intracellular signaling processes should proceed.
• CTL-4 cytotoxic T-lymphocyte antigen 4
• BTLA B and T Lymphocyte Attenuator
• Tim-3 T cell Immunoglobulin and Mucin domain-3
• Lag-3 Lymphocyte Activation Gene-3
• an immune checkpoint inhibitor is an antibody to PD-1.
• PD-1 binds to the programmed cell death 1 receptor (PD-1) to prevent the receptor from binding to the inhibitory ligand PDL-1, thus overriding the ability of tumors to suppress the host anti-tumor immune response.
• PD-1 binds to the programmed cell death 1 receptor (PD-1) to prevent the receptor from binding to the inhibitory ligand PDL-1, thus overriding the ability of tumors to suppress the host anti-tumor immune response.
• the checkpoint inhibitor is a biologic therapeutic or a small molecule.
• the checkpoint inhibitor is a monoclonal antibody, a humanized antibody, a fully human antibody, a fusion protein or a combination thereof.
• the checkpoint inhibitor inhibits a checkpoint protein selected from CTLA-4, PDL1, PDL2, PD1, B7-H3, B7-H4, BTLA, HVEM, TIM3, GAL9, LAG3, VISTA, KIR, 2B4, CD 160, CGEN- 15049, CHK 1, CHK2, A2aR, B-7 family ligands or a combination thereof.
• the checkpoint inhibitor interacts with a ligand of a checkpoint protein selected from CTLA-4, PDL1, PDL2, PD1, B7-H3, B7-H4, BTLA, HVEM, TIM3, GAL9, LAG3, VISTA, KIR, 2B4, CD160, CGEN-15049, CHK 1, CHK2, A2aR, B-7 family ligands or a combination thereof.
• the checkpoint inhibitor is an immunostimulatory agent, a T cell growth factor, an interleukin, an antibody, a vaccine or a combination thereof.
• the interleukin is IL-7 or IL-15.
• the interleukin is glycosylated IL-7.
• the vaccine is a dendritic cell (DC) vaccine.
• DC dendritic cell
• Checkpoint inhibitors include any agent that blocks or inhibits in a statistically significant manner, the inhibitory pathways of the immune system. Such inhibitors may include small molecule inhibitors or may include antibodies, or antigen binding fragments thereof, that bind to and block or inhibit immune checkpoint receptors or antibodies that bind to and block or inhibit immune checkpoint receptor ligands.
• Illustrative checkpoint molecules that may be targeted for blocking or inhibition include, but are not limited to, CTLA-4, PDL1, PDL2, PD1, B7-H3, B7-H4, BTLA, HVEM, GAL9, LAG3, TIM3, VISTA, KIR, 2B4 (belongs to the CD2 family of molecules and is expressed on all NK, gd, and memory CD8 + (ab) T cells), CD160 (also referred to as BY55), CGEN-15049, CHK 1 and CHK2 kinases, A2aR, and various B-7 family ligands.
• CTLA-4 CTLA-4, PDL1, PDL2, PD1, B7-H3, B7-H4, BTLA, HVEM, GAL9, LAG3, TIM3, VISTA, KIR, 2B4 (belongs to the CD2 family of molecules and is expressed on all NK, gd, and memory CD8 + (ab) T cells
• CD160 also referred to as B
• B7 family ligands include, but are not limited to, B7- 1, B7-2, B7-DC, B7-H1, B7-H2, B7-H3, B7-H4, B7-H5, B7-H6 and B7-H7.
• Checkpoint inhibitors include antibodies, or antigen binding fragments thereof, other binding proteins, biologic therapeutics, or small molecules, that bind to and block or inhibit the activity of one or more of CTLA-4, PDL1, PDL2, PD1, BTLA, HVEM, TIM3, GAL9, LAG3, VISTA, KIR, 2B4, CD 160 and CGEN-15049.
• Illustrative immune checkpoint inhibitors include Tremelimumab (CTLA-4 blocking antibody), anti-OX40, PD-L1 monoclonal Antibody (Anti-B7-Hl; MEDI4736), MK-3475 (PD-1 blocker), Nivolumab (anti-PDl antibody), CT-011 (anti-PDl antibody), BY55 monoclonal antibody, AMP224 (anti-PDLl antibody), BMS- 936559 (anti-PDLl antibody), MPLDL3280A (anti-PDLl antibody), MSB0010718C (anti-PDLl antibody), and ipilimumab (anti-CTLA-4 checkpoint inhibitor).
• Checkpoint protein ligands include, but are not limited to PD-L1, PD-L2, B7-H3, B7-H4, CD28, CD86 and TIM-3.
• the immune checkpoint inhibitor is selected from a PD-1 antagonist, a PD-L1 antagonist, and a CTLA-4 antagonist.
• the checkpoint inhibitor is selected from the group consisting of nivolumab (Opdivo®), ipilimumab (Yervoy®), and pembrolizumab (Keytruda®).
• the checkpoint inhibitor is selected from nivolumab (anti -PD-1 antibody, Opdivo®, Bristol-Myers Squibb); pembrolizumab (anti-PD-1 antibody, Keytruda®, Merck); ipilimumab (anti-CTLA-4 antibody, Yervoy®, Bristol-Myers Squibb); durvalumab (anti-PD-Ll antibody, Imfinzi®, AstraZeneca); and atezolizumab (anti-PD-Ll antibody, Tecentriq®, Genentech).
• the checkpoint inhibitor is selected from the group consisting of lambrolizumab (MK-3475), nivolumab (BMS-936558), pidilizumab (CT-011), AMP-224, MDX-1105, MEDI4736, MPDL3280A, BMS-936559, ipilimumab, lirlumab, IPH2101, pembrolizumab (Keytruda®), and tremelimumab.
• MK-3475 lambrolizumab
• BMS-936558 nivolumab
• CT-011 pidilizumab
• AMP-224 pidilizumab
• MDX-1105 MEDI4736
• MPDL3280A MPDL3280A
• BMS-936559 ipilimumab
• lirlumab IPH2101, pembrolizumab (Keytruda®)
• tremelimumab tremelimumab
• an immune checkpoint inhibitor is REGN2810 (Regeneron), an anti- PD-1 antibody tested in patients with basal cell carcinoma (NCT03132636); NSCLC (NCT03088540); cutaneous squamous cell carcinoma (NCT02760498); lymphoma (NCT02651662); and melanoma (NCT03002376); pidilizumab (CureTech), also known as CT-011, an antibody that binds to PD-1, in clinical trials for diffuse large B-cell lymphoma and multiple myeloma; avelumab (Bavencio®, Pfizer/Merck KGaA), also known as MSB0010718C), a fully human IgGl anti-PD-Ll antibody, in clinical trials for non small cell lung cancer, Merkel cell carcinoma, mesothelioma, solid tumors, renal cancer, ovarian cancer, bladder cancer, head and neck cancer, and gastric cancer; or
• Tremelimumab (CP-675,206; Astrazeneca) is a fully human monoclonal antibody against CTLA-4 that has been in studied in clinical trials for a number of indications, including: mesothelioma, colorectal cancer, kidney cancer, breast cancer, lung cancer and non-small cell lung cancer, pancreatic ductal adenocarcinoma, pancreatic cancer, germ cell cancer, squamous cell cancer of the head and neck, hepatocellular carcinoma, prostate cancer, endometrial cancer, metastatic cancer in the liver, liver cancer, large B-cell lymphoma, ovarian cancer, cervical cancer, metastatic anaplastic thyroid cancer, urothelial cancer, fallopian tube cancer, multiple myeloma, bladder cancer, soft tissue sarcoma, and melanoma.
• AGEN-1884 (Agenus) is an anti-CTLA4 antibody that is being studied in Phase 1 clinical trials for advanced solid tumors (NCT02694822).
• a checkpoint inhibitor is an inhibitor of T-cell immunoglobulin mucin containing protein-3 (TIM-3).
• TIM-3 inhibitors that may be used in the present invention include TSR- 022, LY3321367 and MBG453.
• TSR-022 (Tesaro) is an anti -TIM-3 antibody which is being studied in solid tumors (NCT02817633).
• LY3321367 (Eli Lilly) is an anti-TIM-3 antibody which is being studied in solid tumors (NCT03099109).
• MBG453 Novartis
• a checkpoint inhibitor is an inhibitor of T cell immunoreceptor with Ig and ITIM domains, or TIGIT, an immune receptor on certain T cells and NK cells.
• TIGIT inhibitors that may be used in the present invention include BMS-986207 (Bristol-Myers Squibb), an anti-TIGIT monoclonal antibody (NCT02913313); OMP-313M32 (Oncomed); and anti-TIGIT monoclonal antibody (NCT03119428).
• a checkpoint inhibitor is an inhibitor of Lymphocyte Activation Gene- 3 (LAG-3).
• LAG-3 inhibitors that may be used in the present invention include BMS-986016 and REGN3767 and IMP321.
• BMS-986016 (Bristol-Myers Squibb), an anti-LAG-3 antibody, is being studied in glioblastoma and gliosarcoma (NCT02658981).
• REGN3767 (Regeneron), is also an anti-LAG-3 antibody, and is being studied in malignancies (NCT03005782).
• IMP321 is an LAG-3-Ig fusion protein, being studied in melanoma (NCT02676869); adenocarcinoma (NCT02614833); and metastatic breast cancer (NCT00349934).
• Checkpoint inhibitors that may be used in the present invention include 0X40 agonists.
• 0X40 agonists that are being studied in clinical trials include PL-04518600/PL-8600 (Pfizer), an agonistic anti- 0X40 antibody, in metastatic kidney cancer (NCT03092856) and advanced cancers and neoplasms (NCT02554812; NCT05082566); GSK3174998 (Merck), an agonistic anti-OX40 antibody, in Phase 1 cancer trials (NCT02528357); MEDI0562 (Medimmune/AstraZeneca), an agonistic anti-OX40 antibody, in advanced solid tumors (NCT02318394 and NCT02705482); MEDI6469, an agonistic anti-OX40 antibody (Medimmune/AstraZeneca), in patients with colorectal cancer (NCT02559024), breast cancer (NCTO 1862900), head and neck cancer (NCT02274155
• Checkpoint inhibitors that may be used in the present invention include CD 137 (also called 4- 1BB) agonists.
• CD137 agonists that are being studied in clinical trials include utomilumab (PF-05082566, Pfizer) an agonistic anti-CD137 antibody, in diffuse large B-cell lymphoma (NCT02951156) and in advanced cancers and neoplasms (NCT02554812 and NCT05082566); urelumab (BMS-663513, Bristol- Myers Squibb), an agonistic anti-CD137 antibody, in melanoma and skin cancer (NCT02652455) and glioblastoma and gliosarcoma (NCT02658981).
• Checkpoint inhibitors that may be used in the present invention include CD27 agonists.
• CD27 agonists that are being studied in clinical trials include varlilumab (CDX-1127, Celldex Therapeutics) an agonistic anti-CD27 antibody, in squamous cell head and neck cancer, ovarian carcinoma, colorectal cancer, renal cell cancer, and glioblastoma (NCT02335918); lymphomas (NCT01460134); and glioma and astrocytoma (NCT02924038).
• Checkpoint inhibitors that may be used in the present invention include glucocorticoid-induced tumor necrosis factor receptor (GITR) agonists.
• GITR agonists that are being studied in clinical trials include TRX518 (Leap Therapeutics), an agonistic anti-GITR antibody, in malignant melanoma and other malignant solid tumors (NCT01239134 and NCT02628574); GWN323 (Novartis), an agonistic anti-GITR antibody, in solid tumors and lymphoma (NCT 02740270); INCAGN01876 (Incyte/Agenus), an agonistic anti-GITR antibody, in advanced cancers (NCT02697591 and NCT03126110); MK-4166 (Merck), an agonistic anti-GITR antibody, in solid tumors (NCT02132754) and MEDI1873 (Medimmune/AstraZeneca), an agonistic hexameric GITR-ligand molecule with a
• Checkpoint inhibitors that may be used in the present invention include inducible T-cell co stimulator (ICOS, also known as CD278) agonists.
• ICOS agonists that are being studied in clinical trials include MEDI-570 (Medimmune), an agonistic anti-ICOS antibody, in lymphomas (NCT02520791); GSK3359609 (Merck), an agonistic anti-ICOS antibody, in Phase 1 (NCT02723955); JTX-2011 (Jounce Therapeutics), an agonistic anti-ICOS antibody, in Phase 1 (NCT02904226).
• Checkpoint inhibitors that may be used in the present invention include killer IgG-like receptor (KIR) inhibitors.
• KIR killer IgG-like receptor
• KIR inhibitors that are being studied in clinical trials include lirilumab (IPH2102/BMS- 986015, Innate Pharma/Bristol-Myers Squibb), an anti-KIR antibody, in leukemias (NCT01687387, NCT02399917, NCT02481297, NCT02599649), multiple myeloma (NCT02252263), and lymphoma (NCT01592370); IPH2101 (1-7F9, Innate Pharma) in myeloma (NCT01222286 and NCT01217203); and IPH4102 (Innate Pharma), an anti-KIR antibody that binds to three domains of the long cytoplasmic tail (KIR3DL2), in lymphoma (NCT02593045).
• Checkpoint inhibitors that may be used in the present invention include CD47 inhibitors of interaction between CD47 and signal regulatory protein alpha (SIRPa).
• CD47/SIRPa inhibitors that are being studied in clinical trials include ALX-148 (Alexo Therapeutics), an antagonistic variant of (SIRPa) that binds to CD47 and prevents CD47/SIRPa-mediated signaling, in phase 1 (NCT03013218); TTI-621 (SIRPa-Fc, Trillium Therapeutics), a soluble recombinant fusion protein created by linking the N-terminal CD47-binding domain of SIRPa with the Fc domain of human IgGl, acts by binding human CD47, and preventing it from delivering its “do not eat” signal to macrophages, is in clinical trials in Phase 1 (NCT02890368 and NCT02663518); CC-90002 (Celgene), an anti-CD47 antibody, in leukemias
• NCT02641002 Hu5F9-G4 (Forty Seven, Inc.), in colorectal neoplasms and solid tumors (NCT02953782), acute myeloid leukemia (NCT02678338) and lymphoma (NCT02953509).
• Checkpoint inhibitors that may be used in the present invention include CD73 inhibitors.
• CD73 inhibitors that are being studied in clinical trials include MEDI9447 (Medimmune), an anti-CD73 antibody, in solid tumors (NCT02503774); and BMS-986179 (Bristol-Myers Squibb), an anti-CD73 antibody, in solid tumors (NCT02754141).
• Checkpoint inhibitors that may be used in the present invention include agonists of stimulator of interferon genes protein (STING, also known as transmembrane protein 173, or TMEM173).
• STING stimulator of interferon genes protein
• Agonists of STING that are being studied in clinical trials include MK-1454 (Merck), an agonistic synthetic cyclic dinucleotide, in lymphoma (NCT03010176); and ADU-S100 (MIW815, Aduro Biotech/Novartis), an agonistic synthetic cyclic dinucleotide, in Phase 1 (NCT02675439 and NCT03172936).
• Checkpoint inhibitors that may be used in the present invention include CSF1R inhibitors.
• CSF1R inhibitors that are being studied in clinical trials include pexidartinib (PLX3397, Plexxikon), a CSF1R small molecule inhibitor, in colorectal cancer, pancreatic cancer, metastatic and advanced cancers (NCT02777710) and melanoma, non-small cell lung cancer, squamous cell head and neck cancer, gastrointestinal stromal tumor (GIST) and ovarian cancer (NCT02452424); and IMC-CS4 (LY3022855, Lilly), an anti-CSF-lR antibody, in pancreatic cancer (NCT03153410), melanoma (NCT03101254), and solid tumors (NCT02718911); and BLZ945 (4-[2((lR,2R)-2-hydroxycyclohexylamino)-benzothiazol-6- yloxyl]-pyridine-2-carbox
• Checkpoint inhibitors that may be used in the present invention include NKG2A receptor inhibitors.
• NKG2A receptor inhibitors that are being studied in clinical trials include monalizumab (IPH2201, Innate Pharma), an anti-NKG2A antibody, in head and neck neoplasms (NCT02643550) and chronic lymphocytic leukemia (NCT02557516).
• the immune checkpoint inhibitor is selected from nivolumab, pembrolizumab, ipilimumab, avelumab, durvalumab, atezolizumab, or pidilizumab.
• AIBN 2,2'-azo bisisobutyronitrile Anhyd: anhydrous Aq: aqueous
• B2Pni2 bis (pinacolato)diboron -4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(l,3,2-dioxaborolane)
• BINAP 2,2'-bis(diphenylphosphino)- 1 , 1 '-binaphthyl BI3 ⁇ 4: Borane
• Bn benzyl
• Boc fert-butoxycarbonyl B0C2O: di-tert-butyl dicarbonate BPO: benzoyl peroxide "BuOH: n-butanol CDI: carbonyldiimidazole COD: cyclooctadiene d: days
• DABCO 1 ,4-diazobicyclo[2.2.2] octane
• DAST diethylaminosulfur trifluoride
• dba dibenzylideneacetone
• DBU 1,8 -diazobicyclo [5.4.0] undec-7-ene
• DCE 1,2-dichloroethane
• DCM dichloromethane
• DIBAL-H diisobutylaluminum hydride
• DIPEA or DIEA N,N-diisopropylethylamine
• EDC or EDCI l-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride ee: enantiomeric excess
• HATU N,N,N’ ,N’ -tetramethyl-0-(7-azabenzotriazol- l-yl)uronium hexafluorophosphate HC1: hydrochloric acid
• KHMDS potassium hexamethyldisilazide
• NaN02 sodium nitrite
• NBS N-bromosuccinimide
• NFSI N-Fluorobenzenesulfonimide
• NMO N -methyl morpholine N -oxide
• PBS phosphate buffered saline
• PE petroleum ether
• TfAA, TFMSA or Tf 2 0 trifluoromethanesulfonic anhydride
• TIPS triisopropylsilyl
• TMEDA tetramethylethylenediamine
• pTSA para-toluenesulfonic acid
• wt weight
• LCMS was recorded on an Agilent 1200 Series LC/MSD or Shimadzu LCMS2020 equipped with electro-spray ionization and quadruple MS detector [ES+ve to give MH + ] and equipped with Chromolith Flash RP-18e 25*2.0 mm, eluting with 0.0375 vol% TFA in water (solvent A) and 0.01875 vol% TFA in acetonitrile (solvent B).
• Other LCMS was recorded on an Agilent 1290 Infinity RRLC attached with Agilent 6120 Mass detector. The column used was BEH C 1850*2.1 mm, 1.7 micron. Column flow was 0.55 ml /min and mobile phase are used (A) 2 mM Ammonium Acetate in 0.1% Formic Acid in Water and (B) 0.1 % Formic Acid in Acetonitrile.
• LCMS was recorded on an Agilent 1200 Series LC/MSD or Shimadzu LCMS 2020 equipped with electro-spray ionization and quadruple MS detector [ES+ve to give MEL] and equipped with Xbridge C18, 2.1X50 mm columns packed with 5 mm C18-coated silica or Kinetex EVO C18 2.1X30mm columns packed with 5 mm C18-coated silica, eluting with 0.05 vol%NH 3 H 2 0 in water (solvent A) and acetonitrile (solvent B).
• HPLC Analytical Method HPLC was carried out on X Bridge C18 150*4.6 mm, 5 micron. Column flow is 1.0 ml /min and mobile phase are used (A) 0.1 % Ammonia in water and (B) 0.1 % Ammonia in Acetonitrile.
• Prep HPLC Analytical Method The compound was purified on Shimadzu LC-20AP and UV detector. The column used was X-BRIDGE C18 (250* 19)mm, 5m. Column flow was 16.0 ml/min. Mobile phase used was (A) 0.1% Formic Acid in Water and (B) Acetonitrile. Basic method used was (A) 5mM ammonium bicarbonate and 0.1% N3 ⁇ 4 in Water and (B) Acetonitrile or (A) 0.1% Ammonium Hydroxide in Water and (B) Acetonitrile. The UV spectra were recorded at 202nm & 254nm.
• NMR Method The 1H NMR spectra were recorded on a Bruker Ultra Shield Advance 400 MHz/5 mm Probe (BBFO). The chemical shifts are reported in part-per-million.
• Step 1 5-amino-2-(4-fluoro-1.3-dioxoisoindolin-2-yl)-5-oxopentanoic acid
• 4-fluoroisobenzofuran-l,3-dione 25 g, 150 mmol, CAS# 652-39-1
• DMF 100 mL
• L-glutamine 22 g, 150 mmol
• the reaction mixture was heated to at 90 °C and stirred for 2 h.
• the reaction mixture was then evaporated under reduced pressure, transferred into 4 N aqueous HC1 solution and the resulting mixture was stirred for 36 h at rt.
• Step 1 Tert-butyl 6-(cvanomcthylcnc)-2-azaspiro
• Step 2 Tert-butyl 6-(2-aminoethyl)-2-azaspirol3.3 lheptane-2-carbowlate
• tert-butyl 6-(cyanomethylene)-2-azaspiro[3.3]heptane-2-carboxylate 4.10 g, 17.5 mmol
• MeOH 80 mL
• NH3 H 2 0 8 mL
• Raney-Ni 1.50 g, 17.5 mmol
• Step 1 Tert-butyl 6-12-1 l2-(2.6-dio ⁇ o-3-pipcridyl)- 1.3-dio ⁇ o-isoindolin-4-yl laminolcthyl 1-2- azaspiro r3.31heptane-2-carboxylate
• Step 2 4-12-(2-Azaspirol3.31heptan-6-yl)ethylaminol-2-(2.6-dioxo-3-piperidyl)isoindoline- 1.3-dione
• Step 1 ( 1 R.4r)-Mcthyl 4-flivdroxymethvDcvclohexanecarboxylate
• Step 2 (lR.4r)-Methyl 4-((benzyloxy)methyl)cvclohexanecarboxylate
• KOH 4.74 g, 84.5 mmol
• TBAI 4.16 g, 11.3 mmol
• KI 1.87 g, 11.3 mmol
• BnBr 14.5 g, 84.5 mmol, 10.0 mL
• Step 3 (lR.4r)-4-((benzyloxy)methyl)cvclohexanecarboxylic acid
• Step 1 Methyl 5-1
• Step 2 2-l4-(Benzylo ⁇ vmethyl)cvclohe ⁇ yl l-6-bromo- 1.3-benzothi zolc-5-carboxylic acid
• methyl 5-[[4-(benzyloxymethyl)cyclohexanecarbonyl]amino]-2-bromo-4- iodo- benzoate 5.60 g, 9.55 mmol
• DML DML
• Cul 363 mg, 1.91 mmol
• Na2S 93 ⁇ 40 (13.7 g, 57.3 mmol
• Step 3 Methyl 2-l4-(benzylo ⁇ vmethyl)cvclohe ⁇ yl l-6-bromo- 1.3-benzothiazolc-5- carboxylate
• Step 4 Methyl 6-bromo-2-r4-(hvdroxymethyl)cvclohexylT1.3-benzothiazole-5-carboxylate
• BCE 9.88 g, 84.3 mmol
• Step 1 6-(Trifluoromethyl)pyridine-2-carbonyl chloride
• Step 2 6-(Trifluoromethyl)pyridine-2-carboxamide
• a solution of 6-(trifluoromethyl)pyridine-2-carbonyl chloride (21.5 g, 102 mmol) in THF (100 mL) was added NH3 ⁇ 2O (143 g, 1.03 mol, 158 mL, 25% solution) at 0 °C.
• the mixture was stirred at 25 °C for 1 hour.
• the reaction mixture was concentrated in vacuo to remove THF and then fdtered to give the filter cake as title product (19 g, 90% yield) as light yellow solid.
• Step 1 Methyl 2-l4-(hvdro ⁇ vmcthyl)cvclohc ⁇ yl 1-6-1 l6-(trifluoromcthyl)pyridinc-2- carbonyllaminol - 1.3-benzothiazole-5-carboxylate
• Step 2 N-12-14-(hvdroxymethyl)cvclohexylT5-(l-hvdroxy-l-methyl-ethyl)-1.3- benzothiazol-6-yll -6-(trifluoromcthyl)pyridinc-2-carbo ⁇ amidc
• Step 3 N-12-(4-formylcvclohexyl)-5-(l-hvdroxy-l-methyl-ethyl)-1.3-benzothiazol-6-yll-6- (trifluoromethvBpyridine-2-carboxamide
• Step 1 Tert-butyl 2-1 l2-(2.6-dio ⁇ o-3-pipcridyl)- 1.3-dio ⁇ o-isoindolin-4-yl laminol-7- azaspiro G3.51 nonane -7 -carboxylate
• Step 2 4-(7-Azaspiror3.51nonan-2-ylamino)-2-(2.6-dioxo-3-piperidyl)isoindoline-1.3-dione
• Step 1 Methyl 2-sulfanyl-1.3-benzothiazole-5-carboxylate
• Step 2 Methyl 2-methylsulfanyl-1.3-benzothiazole-5-carboxylate
• Step 4 Methyl 2-14-(hvdro ⁇ vmcthyl)- 1 -pipcridyl I- 1.3-bcnzothiazolc-5-carbo ⁇ ylatc
• DMA dimethyl methacrylate
• Step 5 Methyl 6-bromo-2-l4-l(2.2.2-trifluoroacetyl)o ⁇ vmethyl I- 1 -pipcridyl 1-1.3- benzothiazole-5- carboxylate
• Step 6 Methyl 6-bromo-2-14-(hvdroxymethyl)-l-piperidyll-1.3-benzothiazole-5-carboxylate
• methyl 6-bromo-2-[4-[(2,2,2-trifluoroacetyl)oxymethyl]-l-piperidyl]-l,3- benzothiazole-5-carboxylate (2.00 g, 4.16 mmol) in MeOH (60 mL) was added K2CO3 (1.72 g, 12.4 mmol). The mixture was stirred at 25 °C for 2 hours. On completion, the reaction mixture was fdtered and concentrated in vacuo. The residue phase was purified by reverse phase (0.1 % LA condition) to give the title compound (400 mg, 24% yield) as yellow solid.
• LC-MS (ESL) m/z 387.0 (M+3) + .
• Step 1 Methyl 2-14-(hvdro ⁇ vmcthyl )- 1 -pipe ridyl 1-6-1
• Step 2 N-I5-I l-Hvdrow- 1 -mcthyl-cthyl)-2-l4-(hvdro ⁇ vmcthyl)- 1 -piperidyl 1- 1.3- benzothiazol-6-nP -6-(trifluoromethyl)pyridine-2-carboxamide
• Step 3 N-r2-(4-formyl-l-piperidyl)-5-(l-hvdroxy-l-methyl-ethyl)-1.3-benzothiazol-6-yll-6- (trifluoromethyllpyridine-2-carboxamide
• Step 1 Tert-butyl 2-(methylsulfonyloxymethyl)-7-azaspiror3.51nonane-7-carboxylate
• tert-butyl 2-(hydroxymethyl)-7-azaspiro[3.5]nonane-7-carboxylate (1.00 g, 3.92 mmol, CAS# 1356476-27-1) and TEA (594 mg, 5.87 mmol) in DCM (15 mL) was added MsCl (538 mg, 4.70 mmol) at 0 °C. The reaction mixture was stirred at 0 ⁇ 20 °C for 1 hr.
• reaction mixture was diluted with water (30 mL) and extracted with DCM (3 X 40 mL). The combined organic layers were washed with brine (2 X 30 mL), dried over Na 2 S0 4 , fdtered and concentrated in vacuo to give the title compound (1.20 g, 91% yield) as yellow oil.
• Step 2 Tert-butyl 2-(cvanomethyl)-7-azaspirol3.5 lnonane-7-carbowlate
• a solution of tert-butyl 2-(methylsulfonyloxymethyl)-7-azaspiro[3.5]nonane-7-carboxylate (1.20 g, 3.60 mmol) and KI (896 mg, 5.40 mmol) in DMSO (15 mL) was added KCN (257 mg, 3.96 mmol,) at 25 °C.
• the reaction mixture was stirred at 100 °C for 4 hrs. On completion, the reaction mixture was diluted with water (100 mL) and extracted with EA (3 X 60 mL).
• Step 3 Tert-butyl 2-(2-aminoethyl)-7-azaspirol3.51nonane-7-carboxylate
• tert-butyl 2-(cyanomethyl)-7-azaspiro[3.5]nonane-7-carboxylate (0.50 g, 1.89 mmol) and NH 3 ⁇ 2 O (910 mg, 7.27 mmol, 28% solution) in MeOH (10 mL) was added Raney-Ni (32.4 mg, 378 umol). The reaction mixture was stirred at 20 °C for 3 hrs under hydrogen (50 psi).
• Step 1 Tert-butyl 2-12-1
• Step 2 4-12-(7-Azast>irol3.51nonan-2-yl)ethylaminol-2-(2.6-dioxo-3-t>it>eridyl)isoindoline- 1.3-dione
• Step 1 2-Tert-butyl 7-isopropoxy carbonyl 2-azaspirol3.51nonane-2.7-dicarboxylate
• 2-tert-butoxycarbonyl-2-azaspiro[3 5]nonane-7-carboxylic acid 840 mg, 3.12 mmol, CAS# 1363381-18-3
• Et N 1.26 g, 12.5 mmol
• isopropyl carbonochloridate 573 mg, 4.68 mmol.
• the mixture was stirred at 0 °C for 2 hours. On completion, the mixture was fdtered and the filter cake was washed with THF (30 mL). The filtrate was concentrated in vacuo to give the title compound (1.10 g, 99 % yield) as yellow oil.
• Step 2 Tert-butyl 7-(hvdroxymethyl)-2-azaspirol3.51nonane-2-carboxylate
• tert-butyl-isopropoxycarbonyl 2-azaspiro[3.5]nonane-2,7-dicarboxylate (1.10 g, 3.09 mmol) in a mixed solvent of THF (20 mL) and H O (1 mL) was added L1BH4 (404 mg, 18.5 mmol). The mixture was stirred at 0 °C for 2 hours. On completion, the reaction mixture was quenched with water (5.0 mL) at 0 °C, and then extracted with (3 X 20 mL).
• Step 3 Tert-butyl 7-(methylsulfonyloxymethyl)-2-azaspirol3.51nonane-2-carboxylate
• tert-butyl 7-(hydroxymethyl)-2-azaspiro[3.5]nonane-2-carboxylate 800 mg, 3.13 mmol
• Et N 951 mg, 9.40 mmol
• MsCl 43.0 mg, 3.76 mmol
• Step 4 Tert-butyl 7-in.3-dioxoisoindolin-2-yl ' )methyll-2-azaspirol3.51nonane-2-carboxylate
• tert-butyl 7-(methylsulfonyloxymethyl)-2-azaspiro[3.5]nonane-2-carboxylate (1.00 g, 3.00 mmol) in DMF (10 mL) was added (l,3-dioxoisoindolin-2-yl)potassium (833 mg, 4.50 mmol, CAS# 1074-82-4). The mixture was stirred at 80 °C for 2 hours.
• Step 5 Tert-butyl 7-(aminomethyl)-2-azaspirol3.51nonane-2-carboxylate
• NH2NH2.H2O 130 mg, 2.60 mmol
• the mixture was stirred at 80 °C for 2 hours. On completion, the mixture was concentrated in vacuo to give a residue. The residue was washed with DCM (3 X 50 mL) then fdtered, and the organic phase was concentrated in vacuo to give the title compound (100 mg, 75% yield) as colorless oil.
• Step 1 Tert-butyl 7-1112-(2.6-dioxo-3-piperidyl)- 1.3-dio ⁇ o-isoindolin-4 -yllaminolmethyll - 2- azaspiror3.51nonane-2-carboxylate
• Step 2 4-(2-Azaspiror3.51nonan-7-ylmethylamino)-2-(2.6-dioxo-3-piperidyl) isoindoline- 1.3-dione
• Step 1 6-Methylpyridine-2-carbonyl chloride
• Step 2 (lR.4r)-4-(6-Bromo-5-hvdroxybenzoldlthiazol-2-yl)cvclohexanecarboxylic acid
• Step 2 To a solution of methyl 4-[(4-bromo-2-iodo-5-methoxy- phenyl)carbamoyl]cyclohexanecarboxylate (0.8 g, 1.61 mmol) in the DMF (10 mL) was added Na2S 93 ⁇ 40 (774 mg, 3.22 mmol) and Cul (61.4 mg, 322 umol). The mixture was stirred at 80 °C for 12 hrs under N2.
• Step 3 (lR.4r)-Methyl 4-(6-bromo-5-methoxybenzoldlthiazol-2-yl)cvclohexanecarboxylate
• Step 3 To a solution of 4-(6-bromo-5-hydroxy-l,3-benzothiazol-2-yl)cyclohexanecarboxylic acid (567 mg, 1.59 mmol) in the DMF (10 mL) was added K2CO3 (440 mg, 3.19 mmol) and Mel (678 mg, 4.78 mmol). The mixture was stirred at 25 °C for 3 hrs.
• Step 1 Methyl 4-

Landscapes

• Chemical & Material Sciences (AREA)
• Health & Medical Sciences (AREA)
• Organic Chemistry (AREA)
• Life Sciences & Earth Sciences (AREA)
• Pharmacology & Pharmacy (AREA)
• Animal Behavior & Ethology (AREA)
• General Health & Medical Sciences (AREA)
• Public Health (AREA)
• Veterinary Medicine (AREA)
• Medicinal Chemistry (AREA)
• Epidemiology (AREA)
• Engineering & Computer Science (AREA)
• Bioinformatics & Cheminformatics (AREA)
• General Chemical & Material Sciences (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Saccharide Compounds (AREA)
• Liquid Crystal Substances (AREA)
• Enzymes And Modification Thereof (AREA)
• Nitrogen Condensed Heterocyclic Rings (AREA)
• Plural Heterocyclic Compounds (AREA)
• Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
• Medicinal Preparation (AREA)
• Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Priority Applications (11)

Applications Claiming Priority (4)

Publications (1)

Family

ID=76478558

Family Applications (1)

Country Status (14)

Cited By (27)

Families Citing this family (5)

Citations (1)

Family Cites Families (309)