WO2018232055A1 - Aadc polynucleotides for the treatment of parkinson's disease - Google Patents

Aadc polynucleotides for the treatment of parkinson's disease Download PDF

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Publication number
WO2018232055A1
WO2018232055A1 PCT/US2018/037437 US2018037437W WO2018232055A1 WO 2018232055 A1 WO2018232055 A1 WO 2018232055A1 US 2018037437 W US2018037437 W US 2018037437W WO 2018232055 A1 WO2018232055 A1 WO 2018232055A1
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seq
aav
region
aavhu
aavrh
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English (en)
French (fr)
Inventor
Maria SCHEEL
Bernard RAVINA
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Voyager Therapeutics Inc
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Voyager Therapeutics Inc
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Priority to EA201992604A priority Critical patent/EA201992604A1/ru
Priority to PE2019002526A priority patent/PE20200494A1/es
Priority to MX2019014974A priority patent/MX2019014974A/es
Priority to AU2018284960A priority patent/AU2018284960B2/en
Priority to BR112019025122-7A priority patent/BR112019025122A2/pt
Priority to SG11201912154XA priority patent/SG11201912154XA/en
Priority to CN201880052382.9A priority patent/CN111107879A/zh
Priority to EP18816860.3A priority patent/EP3638315A4/en
Priority to JP2019569267A priority patent/JP2020527333A/ja
Priority to CA3065471A priority patent/CA3065471A1/en
Priority to KR1020197036981A priority patent/KR20200018455A/ko
Application filed by Voyager Therapeutics Inc filed Critical Voyager Therapeutics Inc
Priority to US16/184,466 priority patent/US20190060425A1/en
Publication of WO2018232055A1 publication Critical patent/WO2018232055A1/en
Priority to US16/523,567 priority patent/US11759506B2/en
Priority to IL270821A priority patent/IL270821A/en
Priority to CONC2019/0013707A priority patent/CO2019013707A2/es
Priority to PH12019502793A priority patent/PH12019502793A1/en
Anticipated expiration legal-status Critical
Priority to AU2021202378A priority patent/AU2021202378A1/en
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
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    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/86—Viral vectors
    • C12N15/864—Parvoviral vectors, e.g. parvovirus, densovirus
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0075—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the delivery route, e.g. oral, subcutaneous
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    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01028—Aromatic-L-amino-acid decarboxylase (4.1.1.28), i.e. tryptophane-decarboxylase

Definitions

  • the invention relates to compositions, particularly nucleic acid molecules, e.g., polynucleotides encoding AADC, for use in the treatment of Parkinson's disease.
  • AADC polynucleotides may be encoded by or within recombinant adeno- associated viruses (AAVs).
  • AAVs adeno- associated viruses
  • Aromatic L-amino acid decarboxylase is a homodimeric pyridoxal phosphate-dependent enzyme responsible for the synthesis of dopamine and serotonin.
  • the encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (L-DOPA or levodopa) to dopamine; L-5-hydroxytryptophan to serotonin; and L-tryptophan to tryptamine.
  • AADCD aromatic L-amino-acid decarboxylase deficiency
  • Parkinson's Disease is a progressive neurodegenerative disease of the central nervous system (CNS) producing sensory and motor symptoms.
  • Dopamine replacement i.e., levodopa
  • the benefit of dopamine therapy becomes less marked over time, due, in part, to the progressive death of dopamine-generating cells and corresponding loss of AADC activity.
  • systemic administration of high-dose dopamine is complicated by side effects, such as fluctuations in motor performance, dyskinesias, and hallucinations, resulting from dopaminergic stimulation of the mesolimbic system.
  • One strategy to restore dopaminergic function and minimize side effects is the use of gene therapy to deliver AADC directly to a targeted region of the CNS.
  • AAV adeno-associated virus
  • the present invention provides such improved nucleic acid constructs, e.g.,
  • polynucleotides for use with AAV-derived vectors comprising dopa carboxylase (“DDC") gene sequence which encodes a full-length AADC protein for the purpose of gene therapy in the treatment of Parkinson's Disease.
  • DDC dopa carboxylase
  • nucleic acid constructs described herein comprise at least a 5'-ITR and a 3'-ITR, each or both of which may be derived from an AAV, positioned about a DDC gene sequence, as well as additional components required for gene expression and clone selection.
  • compositions, methods, processes, kits and devices for the design, preparation, manufacture and/or formulation of AADC polynucleotides are Described herein.
  • such AADC polynucleotides may be encoded by or contained within plasmids or vectors or recombinant adeno-associated viruses (AAV).
  • AAV adeno-associated viruses
  • FIG. 1 is a schematic of a viral genome of the invention.
  • AAVs Adeno-associated viruses
  • AAV particles Adeno-associated viruses
  • Viruses of the Parvoviridae family are small non-enveloped icosahedral capsid viruses characterized by a single stranded DNA genome. Parvoviridae family viruses consist of two subfamilies: Parvovirinae, which infect vertebrates, and Densovirinae, which infect invertebrates. Due to its relatively simple structure, easily manipulated using standard molecular biology techniques, this virus family is useful as a biological tool.
  • the genome of the virus may be modified to contain a minimum of components for the assembly of a functional recombinant virus, or viral particle, which is loaded with or engineered to express or deliver a desired payload, which may be delivered to a target cell, tissue, organ, or organism.
  • parvoviruses and other members of the Parvoviridae family are generally described in Kenneth I. Berns, "Parvoviridae: The Viruses and Their Replication," Chapter 69 in FIELDS VIROLOGY (3d Ed. 1996), the contents of which are incorporated by reference in their entirety.
  • the Parvoviridae family comprises the Dependovirus genus which includes adeno- associated viruses (AAV) capable of replication in vertebrate hosts including, but not limited to, human, primate, bovine, canine, equine, and ovine species.
  • AAV adeno- associated viruses
  • the vector genome is a linear, single-stranded DNA (ssDNA) molecule approximately 5,000 nucleotides (nt) in length.
  • the AAV viral genome can comprise a payload region and at least one inverted terminal repeat (ITR) or ITR region. ITRs traditionally flank the coding nucleotide sequences for the non- structural proteins (encoded by Rep genes) and the structural proteins (encoded by capsid genes or Cap genes). While not wishing to be bound by theory, an AAV viral genome typically comprises two ITR sequences.
  • the vector genome comprises a characteristic T- shaped hairpin structure defined by the self-complementary terminal 145 nt of the 5' and 3' ends of the ssDNA which form an energetically stable double stranded region.
  • the double stranded hairpin structures comprise multiple functions including, but not limited to, acting as an origin for DNA replication by functioning as primers for the endogenous DNA polymerase complex of the host viral replication cell.
  • AAV particles may comprise the viral genome, in whole or in part, of any naturally occurring and/or recombinant AAV serotype nucleotide sequence or variant.
  • AAV variants may have sequences of significant homology at the nucleic acid (genome or capsid) and amino acid levels (capsids), to produce constructs which are generally physical and functional equivalents, replicate by similar mechanisms, and assemble by similar mechanisms. Chiorini et al., J. Vir. 71 : 6823-33(1997); Srivastava et al., J. Vir. 45:555-64 (1983); Chiorini et al., J. Vir.
  • AAV particles of the present invention are recombinant AAV particles which are replication defective, lacking sequences encoding functional Rep and Cap proteins within their viral genome. These defective AAV particles may lack most or all parental coding sequences and essentially carry only one or two AAV ITR sequences and the nucleic acid of interest for delivery to a cell, a tissue, an organ or an organism.
  • the viral genome of the AAV particles of the present invention comprise at least one control element which provides for the replication, transcription and translation of a coding sequence encoded therein. Not all of the control elements need always be present as long as the coding sequence is capable of being replicated, transcribed and/or translated in an appropriate host cell.
  • expression control elements include sequences for transcription initiation and/or termination, promoter and/or enhancer sequences, efficient RNA processing signals such as splicing and polyadenylation signals, sequences that stabilize cytoplasmic mRNA, sequences that enhance translation efficacy (e.g., Kozak consensus sequence), sequences that enhance protein stability, and/or sequences that enhance protein processing and/or secretion.
  • AAV particles for use in therapeutics and/or diagnostics comprise a virus that has been distilled or reduced to the minimum components necessary for transduction of a nucleic acid payload or cargo of interest.
  • AAV particles are engineered as vehicles for specific delivery while lacking the deleterious replication and/or integration features found in wild-type viruses.
  • AAV particles of the present invention may be produced recombinantly and may be based on adeno-associated virus (AAV) parent or reference sequences.
  • AAV adeno-associated virus
  • a "vector” is any molecule or moiety which transports, transduces or otherwise acts as a carrier of a heterologous molecule such as the nucleic acids described herein.
  • scAAV particles contain DNA strands which anneal together to form double stranded DNA. By skipping second strand synthesis, scAAVs allow for rapid expression in the cell.
  • the AAV particle of the present invention is an scAAV.
  • the AAV particle of the present invention is an ssAAV.
  • AAV particles may be modified to enhance the efficiency of delivery. Such modified AAV particles can be packaged efficiently and be used to successfully infect the target cells at high frequency and with minimal toxicity.
  • the capsids of the AAV particles are engineered according to the methods described in US Publication Number US 20130195801, the contents of which are incorporated herein by reference in their entirety.
  • the AAV particles comprising a payload region encoding the polypeptides of the invention may be introduced into mammalian cells.
  • AAV particles of the present invention may comprise or be derived from any natural or recombinant AAV serotype. According to the present invention, the AAV particles may utilize or be based on a serotype selected from any of the following PHP.B, PHP. A, AAV1, AAV2,
  • AAV16.8/hu. lO AAV16.12/hu. l l, AAV29.3/bb. l, AAV29.5/bb.2, AAV106.1/hu.37,
  • AAV-PAEC AAV-LK01, AAV-LK02, AAV-LK03, AAV-LK04, AAV-LK05, AAV-LK06, AAV- LK07, AAV-LK08, AAV-LK09, AAV-LKIO, AAV-LKl l, AAV-LK12, AAV-LK13, AAV-LK14, AAV-LK15, AAV-LK16, AAV-LK17, AAV-LK18, AAV-LK19, AAV-PAEC2,
  • AAV128.1/hu.43 true type AAV (ttAAV), UPENN AAV 10, Japanese AAV 10 serotypes, AAV CBr-7.1, AAV CBr-7.10, AAV CBr-7.2, AAV CBr-7.3, AAV CBr-7.4, AAV CBr-7.5, AAV CBr-7.7, AAV CBr-7.8, AAV CBr-B7.3, AAV CBr-B7.4, AAV CBr-El, AAV CBr-E2, AAV CBr-E3, AAV CBr-E4,
  • the AAV serotype may be, or have, a modification as described in United States Publication No. US 20160361439, the contents of which are herein incorporated by reference in their entirety, such as but not limited to, Y252F, Y272F, Y444F, Y500F, Y700F, Y704F, Y730F, Y275F, Y281F, Y508F, Y576F, Y612G, Y673F, and Y720F of the wild-type AAVl, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV 12, and hybrids thereof.
  • the AAV serotype may be, or have, a mutation as described in United States Patent No. US 9546112, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, at least two, but not all the F129L, D418E, K53 IE, L584F, V598A and H642N mutations in the sequence of AAV6 (SEQ ID NO:4 of US 9546112), AAVl (SEQ ID NO:6 of US 9546112), AAV2, AAV3, AAV4, AAV5, AAV7, AAV9, AAV10 or AAVl 1 or derivatives thereof.
  • the AAV serotype may be, or have, an AAV6 sequence comprising the K53 IE mutation (SEQ ID NO:5 of US 95461 12).
  • the AAV serotype may be, or have, a mutation in the AAVl sequence, as described in in United States Publication No. US 20130224836, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, at least one of the surface-exposed tyrosine residues, preferably, at positions 252, 273, 445, 701, 705 and 731 of AAVl (SEQ ID NO: 2 of US 20130224836) substituted with another amino acid, preferably with a phenylalanine residue.
  • a mutation in the AAVl sequence as described in in United States Publication No. US 20130224836, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, at least one of the surface-exposed tyrosine residues, preferably, at positions 252, 273, 445, 701, 705 and 731 of AAVl (SEQ ID NO: 2 of US 20130224836) substituted with another amino acid, preferably with a pheny
  • the AAV serotype may be, or have, a mutation in the AAV9 sequence, such as, but not limited to, at least one of the surface-exposed tyrosine residues, preferably, at positions 252, 272, 444, 500, 700, 704 and 730 of AAV2 (SEQ ID NO: 4 of US 20130224836) substituted with another amino acid, preferably with a phenylalanine residue.
  • the tyrosine residue at position 446 of AAV9 (SEQ ID NO: 6 US 20130224836) is substituted with a phenylalanine residue.
  • the serotype may be AAV2 or a variant thereof, as described in International Publication No. WO2016130589, herein incorporated by reference in its entirety.
  • the amino acid sequence of AAV2 may comprise N587A, E548A, or N708A mutations.
  • the amino acid sequence of any AAV may comprise a V708K mutation.
  • the AAV serotype may be, or have, a sequence as described in United States Publication No. US20030138772, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAVl (SEQ ID NO: 6 and 64 of US20030138772), AAV2 (SEQ ID NO: 7 and 70 of US20030138772), AAV3 (SEQ ID NO: 8 and 71 of US20030138772), AAV4 (SEQ ID NO: 63 of US20030138772), AAV5 (SEQ ID NO: 114 of US20030138772), AAV6 (SEQ ID NO: 65 of US20030138772), AAV7 (SEQ ID NO: 1-3 of US20030138772), AAV8 (SEQ ID NO: 4 and 95 of US20030138772), AAV9 (SEQ ID NO: 5 and 100 of US20030138772), AAV10 (SEQ ID NO: 117 of US20030138772, the contents of which are herein
  • the AAV serotype may be, or have, a sequence as described in United States Publication No. US20150159173, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV2 (SEQ ID NO: 7 and 23 of
  • rh.58 SEQ ID NO: 48 of US20150159173), rh.58 (SEQ ID NO: 48 of US20150159173), or variants thereof including, but not limited to Cy5Rl, Cy5R2, Cy5R3, Cy5R4, rh. l3R, rh.37R2, rh.2R, rh.8R, rh.48.1, rh.48.2, rh.48.1.2, hu.44Rl, hu.44R2, hu.44R3, hu.29R, ch.5Rl, rh64Rl, rh64R2, AAV6.2, AAV6.1, AAV6.12, hu.48Rl, hu.48R2, and hu.48R3.
  • the AAV serotype may be, or have, a sequence as described in United States Patent No. US 7198951, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV9 (SEQ ID NO: 1-3 of US 7198951), AAV2 (SEQ ID NO: 4 of US 7198951), AAV1 (SEQ ID NO: 5 of US 7198951), AAV3 (SEQ ID NO: 6 of US 7198951), and AAV8 (SEQ ID NO: 7 of US7198951). [00036] In some embodiments, the AAV serotype may be, or have, a mutation in the AAV9 sequence as described by N Pulichla et al.
  • the AAV serotype may be, or have, a sequence as described in United States Patent No. US 6156303, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV3B (SEQ ID NO: 1 and 10 of US 6156303), AAV6 (SEQ ID NO: 2, 7 and 11 of US 6156303), AAV2 (SEQ ID NO: 3 and 8 of US 6156303), AAV3A (SEQ ID NO: 4 and 9, of US 6156303), or derivatives thereof.
  • AAV3B SEQ ID NO: 1 and 10 of US 6156303
  • AAV6 SEQ ID NO: 2, 7 and 11 of US 6156303
  • AAV2 SEQ ID NO: 3 and 8 of US 6156303
  • AAV3A SEQ ID NO: 4 and 9, of US 6156303
  • the AAV serotype may be, or have, a sequence as described in United States Publication No. US20140359799, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV8 (SEQ ID NO: 1 of US20140359799), AAVDJ (SEQ ID NO: 2 and 3 of US20140359799), or variants thereof.
  • the serotype may be AAVDJ or a variant thereof, such as AAVDJ8 (or AAV-DJ8), as described by Grimm et al. (Journal of Virology 82(12): 5887-5911 (2008), herein incorporated by reference in its entirety).
  • the amino acid sequence of AAVDJ8 may comprise two or more mutations in order to remove the heparin binding domain (HBD).
  • HBD heparin binding domain
  • 7,588,772 may comprise two mutations: (1) R587Q where arginine (R; Arg) at amino acid 587 is changed to glutamine (Q; Gin) and (2) R590T where arginine (R; Arg) at amino acid 590 is changed to threonine (T; Thr).
  • K406R where lysine (K; Lys) at amino acid 406 is changed to arginine (R; Arg)
  • R587Q where arginine (R; Arg) at amino acid 587 is changed to glutamine (Q; Gin)
  • R590T where arginine (R; Arg) at amino acid 590 is changed to threonine (T; Thr).
  • the AAV serotype may be, or have, a sequence of AAV4 as described in International Publication No. WO 1998011244, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to AAV4 (SEQ ID NO: 1-20 of WO1998011244).
  • the AAV serotype may be, or have, a mutation in the AAV2 sequence to generate AAV2G9 as described in International Publication No. WO2014144229 and herein incorporated by reference in its entirety.
  • the AAV serotype may be, or have, a sequence as described in International Publication No. WO2005033321, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to AAV3-3 (SEQ ID NO: 217 of
  • WO2005033321 AAV1 (SEQ ID NO: 219 and 202 of WO2005033321), AAV106.1/hu.37 (SEQ ID No: 10 of WO2005033321), AAV114.3/hu.40 (SEQ ID No: 11 of WO2005033321),
  • AAV127.2/hu.41 (SEQ ID NO:6 and 8 of WO2005033321), AAV128.3/hu.44 (SEQ ID No: 81 of WO2005033321), AAV130.4/hu.48 (SEQ ID NO: 78 of WO2005033321), AAV145.1/hu.53 (SEQ ID No: 176 and 177 of WO2005033321), AAV145.6/hu.56 (SEQ ID NO: 168 and 192 of
  • WO2005033321 AAV16.12/hu. l l (SEQ ID NO: : 153 and 57 of WO2005033321),
  • AAV16.8/hu. lO (SEQ ID NO: : 156 and 56 of WO2005033321), AAV161.10/hu.60 (SEQ ID No: 170 of WO2005033321), AAV161.6/hu.61 (SEQ ID No: 174 of WO2005033321), AAVl-7/rh.48 (SEQ ID NO: 32 of WO2005033321), AAVl-8/rh.49 (SEQ ID NOs: 103 and 25 of
  • WO2005033321 AAV2 (SEQ ID NO: 211 and 221 of WO2005033321), AAV2-15/rh.62 (SEQ ID No: 33 and 114 of WO2005033321), AAV2-3/rh.61 (SEQ ID NO: 21 of WO2005033321), AAV2- 4/rh.50 (SEQ ID No: 23 and 108 of WO2005033321), AAV2-5/rh.51 (SEQ ID NO: 104 and 22 of WO2005033321), AAV3.1/hu.6 (SEQ ID NO: 5 and 84 of WO2005033321), AAV3.1/hu.9 (SEQ ID NO: 155 and 58 of WO2005033321), AAV3-1 l/rh.53 (SEQ ID NO: 186 and 176 of
  • WO2005033321 AAV3-3 (SEQ ID NO: 200 of WO2005033321), AAV33.12/hu. l7 (SEQ ID NO:4 of WO2005033321), AAV33.4/hu. l5 (SEQ ID No: 50 of WO2005033321), AAV33.8/hu.
  • WO2005033321 AAV58.2/hu.25 (SEQ ID No: 49 of WO2005033321), AAV6 (SEQ ID NO: 203 and 220 of WO2005033321), AAV7 (SEQ ID NO: 222 and 213 of WO2005033321), AAV7.3/hu.7 (SEQ ID No: 55 of WO2005033321), AAV8 (SEQ ID NO: 223 and 214 of WO2005033321), AAVH-l/hu. l (SEQ ID No: 46 of WO2005033321), AAVH-5/hu.3 (SEQ ID No: 44 of WO2005033321), AAVhu.
  • AAVhu. l4/AAV9 (SEQ ID NO: 123 and 3 of WO2005033321), AAVhu.15 (SEQ ID NO: 147 of WO2005033321), AAVhu.16 (SEQ ID NO: 148 of WO2005033321), AAVhu.17 (SEQ ID NO: 83 of WO2005033321), AAVhu.18 (SEQ ID NO: 149 of WO2005033321), AAVhu.19 (SEQ ID NO: 133 of WO2005033321), AAVhu.2 (SEQ ID NO: 143 of WO2005033321), AAVhu.20 (SEQ ID NO: 134 of WO2005033321), AAVhu.21 (SEQ ID NO: 135 of WO2005033321), AAVhu.22 (SEQ ID NO: 138 of WO2005033321), AAVhu.23.2 (SEQ ID NO: 137 of WO2005033321), AAVh
  • WO2005033321 AAVhu.3 (SEQ ID NO: 145 of WO2005033321), AAVhu.31 (SEQ ID NO: 121 of WO2005033321), AAVhu.32 (SEQ ID NO: 122 of WO2005033321), AAVhu.34 (SEQ ID NO: 125 of WO2005033321), AAVhu.35 (SEQ ID NO: 164 of WO2005033321), AAVhu.37 (SEQ ID NO: 88 of WO2005033321), AAVhu.39 (SEQ ID NO: 102 of WO2005033321), AAVhu.4 (SEQ ID NO: 141 of WO2005033321), AAVhu.40 (SEQ ID NO: 87 of WO2005033321), AAVhu.41 (SEQ ID NO: 91 of WO2005033321), AAVhu.42 (SEQ ID NO: 85 of WO2005033321), AAVhu.43 (
  • WO2005033321 AAVhu.49 (SEQ ID NO: 189 of WO2005033321), AAVhu.51 (SEQ ID NO: 190 of WO2005033321), AAVhu.52 (SEQ ID NO: 191 of WO2005033321), AAVhu.53 (SEQ ID NO: 186 of WO2005033321), AAVhu.54 (SEQ ID NO: 188 of WO2005033321), AAVhu.55 (SEQ ID NO: 187 of WO2005033321), AAVhu.56 (SEQ ID NO: 192 of WO2005033321), AAVhu.57 (SEQ ID NO: 193 of WO2005033321), AAVhu.58 (SEQ ID NO: 194 of WO2005033321), AAVhu.6 (SEQ ID NO: 84 of WO2005033321), AAVhu.60 (SEQ ID NO: 184 of WO2005033321),
  • AAVhu.61 (SEQ ID NO: 185 of WO2005033321), AAVhu.63 (SEQ ID NO: 195 of
  • WO2005033321 AAVhu.64 (SEQ ID NO: 196 of WO2005033321), AAVhu.66 (SEQ ID NO: 197 of WO2005033321), AAVhu.67 (SEQ ID NO: 198 of WO2005033321), AAVhu.7 (SEQ ID NO: 150 of WO2005033321), AAVhu.8 (WO2005033321 SEQ ID NO: 12), AAVhu.9 (SEQ ID NO: (SEQ ID NO: 86 of WO2005033321), AAVLG-4/rh.38 (SEQ ID No: 7 of WO2005033321), AAVN721-8/rh.43 (SEQ ID NO: 163 of WO2005033321), AAVN721-8/rh.43 (SEQ ID No: 43 of WO2005033321), AAVpi.
  • WO2005033321 WO2005033321
  • AAVrh.40 SEQ ID NO: 92 of WO2005033321
  • AAVrh.43 SEQ ID NO: 163 of WO2005033321
  • AAVrh.44 WO2005033321 SEQ ID NO: 34
  • AAVrh.45 WO2005033321 SEQ ID NO: 41
  • AAVrh.47 WO2005033321 SEQ ID NO: 38
  • AAVrh.48 SEQ ID NO: 115 of WO2005033321
  • AAVrh.49 SEQ ID NO: 103 of WO2005033321
  • AAVrh.50 SEQ ID NO: 108 of WO2005033321
  • AAVrh.51 SEQ ID NO: 104 of WO2005033321
  • AAVrh.52 SEQ ID NO: 96 of WO2005033321
  • AAVrh.53 SEQ ID NO: 97 of WO2005033321
  • AAVrh.59 (WO2005033321 SEQ ID NO: 42), AAVrh.60 (WO2005033321 SEQ ID NO: 31), AAVrh.61 (SEQ ID NO: 107 of WO2005033321), AAVrh.62 (SEQ ID NO: 114 of
  • WO2005033321 WO2005033321
  • AAVrh.64 SEQ ID NO: 99 of WO2005033321
  • AAVrh.65 WO2005033321 SEQ ID NO: 35
  • AAVrh.68 WO2005033321 SEQ ID NO: 16
  • AAVrh.69 WO2005033321 SEQ ID NO: 39
  • AAVrh.70 WO2005033321 SEQ ID NO: 20
  • AAVrh.72 WO2005033321 SEQ ID NO: 9
  • variants thereof including, but not limited to, AAVcy.2, AAVcy.3, AAVcy.4, AAVcy.5, AAVcy.6, AAVrh.12, AAVrh.17, AAVrh.18, AAVrh.19, AAVrh.21, AAVrh.22, AAVrh.23, AAVrh.24, AAVrh.25, AAVrh.25/42 15, AAVrh.31,
  • Non limiting examples of variants include SEQ ID NO: 13, 15, 17, 19, 24, 36, 40, 45, 47, 48, 51-54, 60-62, 64-77, 79, 80, 82, 89, 90, 93-95, 98, 100, 101, , 109- 113, 118-120, 124, 126, 131, 139, 142, 151, 154, 158, 161, 162, 165-183, 202, 204-212, 215, 219, 224-236, of WO2005033321, the contents of which are herein incorporated by reference in their entirety.
  • the AAV serotype may be, or have, a sequence as described in International Publication No. WO2015168666, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAVrh8R (SEQ ID NO: 9 of
  • the AAV serotype may be, or have, a sequence as described in United States Patent No. US9233131, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAVhEl .
  • the AAV serotype may be, or have, a sequence as described in United States Patent Publication No. US20150376607, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV-PAEC (SEQ ID NO: 1 of
  • the AAV serotype may be, or have, a sequence as described in United States Patent No. US9163261, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV-2-pre-miRNA-101 (SEQ ID NO: 1 US9163261), or variants thereof.
  • the AAV serotype may be, or have, a sequence as described in United States Patent Publication No. US20150376240, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV-8h (SEQ ID NO: 6 of
  • US20150376240 AAV-8b (SEQ ID NO: 5 of US20150376240), AAV-h (SEQ ID NO: 2 of US20150376240), AAV-b (SEQ ID NO: 1 of US20150376240), or variants thereof.
  • the AAV serotype may be, or have, a sequence as described in United States Patent Publication No. US20160017295, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV SM 10-2 (SEQ ID NO: 22 of US20160017295), AAV Shuffle 100-1 (SEQ ID NO: 23 of US20160017295), AAV Shuffle 100-3 (SEQ ID NO: 24 of US20160017295), AAV Shuffle 100-7 (SEQ ID NO: 25 of US20160017295), AAV Shuffle 10-2 (SEQ ID NO: 34 of US20160017295), AAV Shuffle 10-6 (SEQ ID NO: 35 of US20160017295), AAV Shuffle 10-8 (SEQ ID NO: 36 of US20160017295), AAV Shuffle 100-2 (SEQ ID NO: 37 of US20160017295), AAV SM 10-1 (SEQ ID NO: 38 of US20160017295), AAV SM 10-8 (SEQ ID NO:
  • the AAV serotype may be, or have, a sequence as described in United States Patent Publication No. US20150238550, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, BNP61 AAV (SEQ ID NO: 1 of
  • BNP62 AAV SEQ ID NO: 3 of US20150238550
  • BNP63 AAV SEQ ID NO: 4 of US20150238550
  • the AAV serotype may be or may have a sequence as described in United States Patent Publication No. US20150315612, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAVrh.50 (SEQ ID NO: 108 of
  • AAV54.4R/hu.27 (SEQ ID No: 64 of US20150315612), AAV46.2/hu.28 (SEQ ID No: 68 of US20150315612), AAV46.6/hu.29 (SEQ ID No: 69 of US20150315612), AAV128.1/hu.43 (SEQ ID No: 80 of US20150315612), or variants thereof.
  • the AAV serotype may be, or have, a sequence as described in International Publication No. WO2015121501, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, true type AAV (ttAAV) (SEQ ID NO: 2 of WO2015121501), "UPenn AAV10” (SEQ ID NO: 8 of WO2015121501), “Japanese AAV10” (SEQ ID NO: 9 of WO2015121501), or variants thereof.
  • true type AAV ttAAV
  • UPenn AAV10 SEQ ID NO: 8 of WO2015121501
  • Japanese AAV10 Japanese AAV10
  • AAV capsid serotype selection or use may be from a variety of species.
  • the AAV may be an avian AAV (AAAV).
  • the AAAV serotype may be, or have, a sequence as described in United States Patent No. US 9238800, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAAV (SEQ ID NO: 1, 2, 4, 6, 8, 10, 12, and 14 of US 9,238,800), or variants thereof.
  • the AAV may be a bovine AAV (BAAV).
  • BAAV serotype may be, or have, a sequence as described in United States Patent No. US 9,193,769, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, BAAV (SEQ ID NO: 1 and 6 of US 9193769), or variants thereof.
  • BAAV serotype may be or have a sequence as described in United States Patent No. US7427396, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, BAAV (SEQ ID NO: 5 and 6 of US7427396), or variants thereof.
  • the AAV may be a caprine AAV.
  • the caprine AAV serotype may be, or have, a sequence as described in United States Patent No. US7427396, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, caprine AAV (SEQ ID NO: 3 of US7427396), or variants thereof.
  • the AAV may be engineered as a hybrid AAV from two or more parental serotypes.
  • the AAV may be AAV2G9 which comprises sequences from AAV2 and AAV9.
  • the AAV2G9 AAV serotype may be, or have, a sequence as described in United States Patent Publication No. US20160017005, the contents of which are herein incorporated by reference in its entirety.
  • the AAV may be a serotype generated by the AAV9 capsid library with mutations in amino acids 390-627 (VP1 numbering) as described by Pulichla et al.
  • the serotype and corresponding nucleotide and amino acid substitutions may be, but is not limited to, AAV9.1 (G1594C; D532H), AAV6.2 (T1418A and T1436X; V473D and I479K), AAV9.3 (T1238A; F413Y), AAV9.4 (T1250C and A1617T; F417S), AAV9.5
  • the AAV serotype may be, or have, a sequence as described in International Publication No. WO2016049230, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to AAVF1/HSC1 (SEQ ID NO: 2 and 20 of WO2016049230), AAVF2/HSC2 (SEQ ID NO: 3 and 21 of WO2016049230), AAVF3/HSC3 (SEQ ID NO: 5 and 22 of WO2016049230), AAVF4/HSC4 (SEQ ID NO: 6 and 23 of WO2016049230), AAVF5/HSC5 (SEQ ID NO: 11 and 25 of WO2016049230), AAVF6/HSC6 (SEQ ID NO: 7 and 24 of WO2016049230), AAVF7/HSC7 (SEQ ID NO: 8 and 27 of WO2016049230), AAVF8/HSC8 (SEQ ID NO: 9 and 28 of WO20160490
  • WO2016049230 AAVF11/HSC11 (SEQ ID NO: 4 and 26 of WO2016049230), AAVF12/HSC12 (SEQ ID NO: 12 and 30 of WO2016049230), AAVF13/HSC13 (SEQ ID NO: 14 and 31 of
  • WO2016049230 AAVF14/HSC14 (SEQ ID NO: 15 and 32 of WO2016049230), AAVF15/HSC15 (SEQ ID NO: 16 and 33 of WO2016049230), AAVF16/HSC16 (SEQ ID NO: 17 and 34 of
  • WO2016049230 AAVF17/HSC17 (SEQ ID NO: 13 and 35 of WO2016049230), or variants or derivatives thereof.
  • the AAV serotype may be, or have, a sequence as described in United States Patent No. US 8734809, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, AAV CBr-El (SEQ ID NO: 13 and 87 of US8734809), AAV CBr-E2 (SEQ ID NO: 14 and 88 of US8734809), AAV CBr-E3 (SEQ ID NO: 15 and 89 of US8734809), AAV CBr-E4 (SEQ ID NO: 16 and 90 of US8734809), AAV CBr-E5 (SEQ ID NO: 17 and 91 of US8734809), AAV CBr-e5 (SEQ ID NO: 18 and 92 of US8734809), AAV CBr-E6 (SEQ ID NO: 19 and 93 of US8734809), AAV CBr-E7 (SEQ ID NO: 20 and 94 of US87
  • the AAV serotype may be, or have, a sequence as described in International Publication No. WO2016065001, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to AAV CHt-P2 (SEQ ID NO: 1 and 51 of WO2016065001), AAV CHt-P5 (SEQ ID NO: 2 and 52 of WO2016065001), AAV CHt-P9 (SEQ ID NO: 3 and 53 of WO2016065001), AAV CBr-7.1 (SEQ ID NO: 4 and 54 of WO2016065001), AAV CBr-7.2 (SEQ ID NO: 5 and 55 of WO2016065001), AAV CBr-7.3 (SEQ ID NO: 6 and 56 of WO2016065001), AAV CBr-7.4 (SEQ ID NO: 7 and 57 of WO2016065001), AAV CBr-7.5 (SEQ ID NO: 8 and 58 of WO2016065001),
  • WO2016065001 AAV CLv-L4 (SEQ ID NO: 15 and 65 of WO2016065001), AAV CLv-L5 (SEQ ID NO: 16 and 66 of WO2016065001), AAV CLv-L6 (SEQ ID NO: 17 and 67 of WO2016065001), AAV CLv-Kl (SEQ ID NO: 18 and 68 of WO2016065001), AAV CLv-K3 (SEQ ID NO: 19 and 69 of WO2016065001), AAV CLv-K6 (SEQ ID NO: 20 and 70 of WO2016065001), AAV CLv-Ml (SEQ ID NO: 21 and 71 of WO2016065001), AAV CLv-Ml 1 (SEQ ID NO: 22 and 72 of
  • WO2016065001 AAV CLv-M2 (SEQ ID NO: 23 and 73 of WO2016065001), AAV CLv-M5 (SEQ ID NO: 24 and 74 of WO2016065001), AAV CLv-M6 (SEQ ID NO: 25 and 75 of
  • WO2016065001 AAV CLv-M7 (SEQ ID NO: 26 and 76 of WO2016065001), AAV CLv-M8 (SEQ ID NO: 27 and 77 of WO2016065001), AAV CLv-M9 (SEQ ID NO: 28 and 78 of
  • WO2016065001 AAV CHt-Pl (SEQ ID NO: 29 and 79 of WO2016065001), AAV CHt-P6 (SEQ ID NO: 30 and 80 of WO2016065001), AAV CHt-P8 (SEQ ID NO: 31 and 81 of WO2016065001), AAV CHt-6.1 (SEQ ID NO: 32 and 82 of WO2016065001), AAV CHt-6.10 (SEQ ID NO: 33 and 83 of WO2016065001), AAV CHt-6.5 (SEQ ID NO: 34 and 84 of WO2016065001), AAV CHt-6.6 (SEQ ID NO: 35 and 85 of WO2016065001), AAV CHt-6.7 (SEQ ID NO: 36 and 86 of
  • WO2016065001 AAV CSp-8.4 (SEQ ID NO: 40 and 90 of WO2016065001), AAV CSp-8.5 (SEQ ID NO: 41 and 91 of WO2016065001), AAV CSp-8.6 (SEQ ID NO: 42 and 92 of WO2016065001), AAV CSp-8.7 (SEQ ID NO: 43 and 93 of WO2016065001), AAV CSp-8.8 (SEQ ID NO: 44 and 94 of WO2016065001), AAV CSp-8.9 (SEQ ID NO: 45 and 95 of WO2016065001), AAV CBr-B7.3 (SEQ ID NO: 46 and 96 of WO2016065001), AAV CBr-B7.4 (SEQ ID NO: 47 and 97 of
  • WO2016065001 AAV3B (SEQ ID NO: 48 and 98 of WO2016065001), AAV4 (SEQ ID NO: 49 and 99 of WO2016065001), AAV5 (SEQ ID NO: 50 and 100 of WO2016065001), or variants or derivatives thereof.
  • the AAV serotype may be, or have, a modification as described in United States Publication No. US 20160361439, the contents of which are herein incorporated by reference in their entirety, such as but not limited to, Y252F, Y272F, Y444F, Y500F, Y700F, Y704F, Y730F, Y275F, Y281F, Y508F, Y576F, Y612G, Y673F, and Y720F of the wild-type AAVl, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV10, AAV11, AAV 12, and hybrids thereof.
  • the AAV serotype may be, or have, a mutation as described in United States Patent No. US 9546112, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, at least two, but not all the F129L, D418E, K53 IE, L584F, V598A and H642N mutations in the sequence of AAV6 (SEQ ID NO:4 of US 9546112), AAVl (SEQ ID NO:6 of US 9546112), AAV2, AAV3, AAV4, AAV5, AAV7, AAV9, AAV10 or AAVl 1 or derivatives thereof.
  • the AAV serotype may be, or have, an AAV6 sequence comprising the K53 IE mutation (SEQ ID NO:5 of US 95461 12).
  • the AAV serotype may be, or have, a mutation in the AAVl sequence, as described in in United States Publication No. US 20130224836, the contents of which are herein incorporated by reference in their entirety, such as, but not limited to, at least one of the surface-exposed tyrosine residues, preferably, at positions 252, 273, 445, 701, 705 and 731 of AAVl (SEQ ID NO: 2 of US 20130224836) substituted with another amino acid, preferably with a phenylalanine residue.
  • the AAV serotype may be, or have, a mutation in the AAV9 sequence, such as, but not limited to, at least one of the surface-exposed tyrosine residues, preferably, at positions 252, 272, 444, 500, 700, 704 and 730 of AAV2 (SEQ ID NO: 4 of US 20130224836) substituted with another amino acid, preferably with a phenylalanine residue.
  • the tyrosine residue at position 446 of AAV9 (SEQ ID NO: 6 US 20130224836) is substituted with a phenylalanine residue.
  • the serotype may be AAV2 or a variant thereof, as described in International Publication No.
  • the amino acid sequence of AAV2 may comprise N587A, E548A, or N708A mutations. In one embodiment, the amino acid sequence of any AAV may comprise a V708K mutation.
  • the AAV may be a serotype selected from any of those found in Table 1.
  • the AAV may comprise a sequence, fragment or variant thereof, of the sequences in Table 1.
  • the AAV may be encoded by a sequence, fragment or variant as described in Table 1.
  • AAVcy.2 (AAV13.3) 145 US20030138772 SEQ ID NO 15
  • AAVcy.3 (AAV24.1) 147 US20030138772 SEQ ID NO 16
  • AAVcy.4 (AAV27.3) 149 US20030138772 SEQ ID NO 17
  • AAVcy.5 (AAV7.2) 152 US20030138772 SEQ ID NO 18
  • AAVcy.6 (AAV16.3) 154 US20030138772 SEQ ID NO 10
  • AAVhErl.23 (AAVhEr2.29) 172 US9233131 SEQ ID NO: 53
  • AAVhu. lO (AAV16.8) 186 US20150315612 SEQ ID NO: 56
  • AAVhu.10 (AAV16.8) 187 US20150315612 SEQ ID NO: 156
  • AAVhu. l l (AAV16.12) 188 US20150315612 SEQ ID NO: 57
  • AAVhu.15 (AAV33.4) 199 US20150315612 SEQ ID NO: 50
  • AAVhu.16 (AAV33.8) 202 US20150315612 SEQ ID NO: 51
  • AAVhu.17 (AAV33.12) 204 US20150315612 SEQ ID NO: 4
  • AAVhu.39 (AAVLG-9) 257 US20150315612 SEQ ID NO: 24
  • AAVhu.40 (AAV114.3) 261 US20150315612 SEQ ID No: 11
  • AAVhu.41 (AAV127.2) 263 US20150315612 SEQ ID NO: 6
  • AAVhu.42 (AAV127.5) 265 US20150315612 SEQ ID NO: 8
  • AAVhu.43 (AAV128.1) 268 US20150315612 SEQ ID NO: 80
  • AAVhu.44 (AAV128.3) 270 US20150315612 SEQ ID NO: 81
  • AAVhu.48 (AAV130.4) 283 US20150315612 SEQ ID NO: 78
  • AAVhu.53 (AAV145.1) 297 US20150315612 SEQ ID NO 176
  • AAVhu.54 (AAV145.5) 299 US20150315612 SEQ ID No: 177
  • AAVhu.56 (AAV145.6) 302 US20150315612 SEQ ID NO: 168
  • AAVhu.56 (AAV145.6) 303 US20150315612 SEQ ID NO: 192
  • AAVhu.6 (AAV3.1) 309 US20150315612 SEQ ID NO: 5
  • AAVhu.6 (AAV3.1) 310 US20150315612 SEQ ID NO: 84
  • AAVhu.60 (AAV161.10) 312 US20150315612 SEQ ID NO: 170
  • AAVhu.61 (AAV161.6) 314 US20150315612 SEQ ID NO: 174
  • AAVhu.7 (AAV7.3) 324 US20150315612 SEQ ID NO: 55
  • AAVhu.9 (AAV3.1) 329 US20150315612 SEQ ID NO: 58
  • AAVhu.9 (AAV3.1) 330 US20150315612 SEQ ID NO: 155
  • AAV-LK06 342 US20150376607 SEQ ID NO: 34
  • AAVrh.10 (AAV44.2) 396 US20030138772 SEQ ID NO: 81
  • AAVrh.12 (AAV42.1b) 398 US20030138772 SEQ ID NO: 30
  • AAVrh.14 (AAV42.3a) 404 US20030138772 SEQ ID NO: 32
  • AAVrh.17 (AAV42.5a) 406 US20030138772 SEQ ID NO: 34
  • AAVrh.18 (AAV42.5b) 408 US20030138772 SEQ ID NO: 29
  • AAVrh.19 (AAV42.6b) 410 US20030138772 SEQ ID NO: 38
  • AAVrh.21 (AAV42.10) 415 US20030138772 SEQ ID NO: 35
  • AAVrh.48 (AAV1-7) 460 US20150315612 SEQ ID NO: 32
  • AAVrh.49 (AAV1-8) 461 US20150315612 SEQ ID NO: 25
  • AAVrh.49 (AAV1-8) 462 US20150315612 SEQ ID NO: 103
  • AAVrh.50 (AAV2-4) 463 US20150315612 SEQ ID NO: 23
  • AAVrh.50 (AAV2-4) 464 US20150315612 SEQ ID NO: 108
  • AAVrh.51 (AAV2-5) 465 US20150315612 SEQ ID No: 22
  • AAVrh.51 (AAV2-5) 466 US20150315612 SEQ ID NO: 104
  • AAVrh.52 (AAV3-9) 467 US20150315612 SEQ ID NO: 18
  • AAVrh.52 (AAV3-9) 468 US20150315612 SEQ ID NO: 96
  • AAVrh.53 (AAV3-11) 470 US20150315612 SEQ ID NO: 17
  • AAVrh.53 (AAV3-11) 471 US20150315612 SEQ ID NO: 186
  • AAVrh.55 (AAV4-19) 475 US20150315612 SEQ ID NO: 117
  • AAVrh.61 (AAV2-3) 488 US20150315612 SEQ ID NO: 21
  • AAVrh.62 (AAV2-15) 489 US20150315612 SEQ ID No: 33
  • AAVrh.62 (AAV2-15) 490 US20150315612 SEQ ID NO: 114
  • BAAV bovine AAV 516 US9193769 SEQ ID NO: 8
  • BAAV bovine AAV 517 US9193769 SEQ ID NO: 10
  • BAAV bovine AAV 518 US9193769 SEQ ID NO: 4
  • BAAV bovine AAV 520 US9193769 SEQ ID NO: 6
  • BAAV bovine AAV 521 US9193769 SEQ ID NO: 1
  • BAAV bovine AAV 522 US9193769 SEQ ID NO: 5
  • BAAV bovine AAV 523 US9193769 SEQ ID NO: 3
  • BAAV bovine AAV 525 US7427396 SEQ ID NO: 5
  • BAAV bovine AAV 526 US7427396 SEQ ID NO: 6
  • AAAV (Avian AAV) 536 US9238800 SEQ ID NO: 12
  • AAAV (Avian AAV) 537 US9238800 SEQ ID NO: 2
  • AAAV (Avian AAV) 538 US9238800 SEQ ID NO: 6
  • AAAV (Avian AAV) 539 US9238800 SEQ ID NO: 4
  • AAAV (Avian AAV) 540 US9238800 SEQ ID NO: 8
  • AAAV (Avian AAV) 541 US9238800 SEQ ID NO: 14
  • AAAV (Avian AAV) 542 US9238800 SEQ ID NO: 10
  • AAAV (Avian AAV) 543 US9238800 SEQ ID NO: 15
  • AAAV (Avian AAV) 544 US9238800 SEQ ID NO: 5
  • AAAV (Avian AAV) 545 US9238800 SEQ ID NO: 9
  • AAAV (Avian AAV) 546 US9238800 SEQ ID NO: 3
  • AAAV (Avian AAV) 547 US9238800 SEQ ID NO: 7
  • AAAV (Avian AAV) 548 US9238800 SEQ ID NO: 11
  • AAAV (Avian AAV) 549 US9238800 SEQ ID NO: 13
  • AAAV (Avian AAV) 550 US9238800 SEQ ID NO: 1
  • AAV3B 870 WO2016065001 SEQ ID NO: 98
  • rh74 (RHM4-1) 1017 US2015023924A1 SEQ ID NO: 11 rh74 (RHM15-1) 1018 US2015023924A1 SEQ ID NO 12 rh74 (RHM15-2) 1019 US2015023924A1 SEQ ID NO 13 rh74 (RHM15-3/RHM15-5) 1020 US2015023924A1 SEQ ID NO 14 rh74 (RHM15-4) 1021 US2015023924A1 SEQ ID NO 15 rh74 (RHM15-6) 1022 US2015023924A1 SEQ ID NO 16
  • AAV2 (comprising lung
  • AAV2 (comprising lung

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MX2019014974A MX2019014974A (es) 2017-06-15 2018-06-14 Polinucleotidos de aadc para el tratamiento de la enfermedad de parkinson.
AU2018284960A AU2018284960B2 (en) 2017-06-15 2018-06-14 AADC polynucleotides for the treatment of Parkinson's disease
BR112019025122-7A BR112019025122A2 (pt) 2017-06-15 2018-06-14 polinucleotídeos de aadc para o tratamento de mal de parkinson
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