WO2000044895A1 - Verfahren und medikament zur hemmung der expression eines vorgegebenen gens - Google Patents

Verfahren und medikament zur hemmung der expression eines vorgegebenen gens Download PDF

Info

Publication number
WO2000044895A1
WO2000044895A1 PCT/DE2000/000244 DE0000244W WO0044895A1 WO 2000044895 A1 WO2000044895 A1 WO 2000044895A1 DE 0000244 W DE0000244 W DE 0000244W WO 0044895 A1 WO0044895 A1 WO 0044895A1
Authority
WO
WIPO (PCT)
Prior art keywords
dsrna
complementary
target gene
use according
region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2000/000244
Other languages
German (de)
English (en)
French (fr)
Inventor
Roland Kreutzer
Stephan Limmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26051592&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2000044895(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to EP02003683.6A priority Critical patent/EP1214945B2/de
Application filed by Individual filed Critical Individual
Priority to DE50000414T priority patent/DE50000414D1/de
Priority to DK05002454T priority patent/DK1550719T3/da
Priority to AT00910510T priority patent/ATE222953T1/de
Priority to DE10080167T priority patent/DE10080167B4/de
Priority to AU2000032713A priority patent/AU778474B2/en
Priority to CA002359180A priority patent/CA2359180C/en
Priority to EP00910510A priority patent/EP1144623B9/de
Priority to JP2000596137A priority patent/JP2003502012A/ja
Publication of WO2000044895A1 publication Critical patent/WO2000044895A1/de
Anticipated expiration legal-status Critical
Priority to US10/383,099 priority patent/US8119608B2/en
Priority to US10/382,768 priority patent/US8168776B2/en
Priority to US10/382,395 priority patent/US8101584B2/en
Priority to US10/384,339 priority patent/US7829693B2/en
Priority to US10/612,179 priority patent/US8202980B2/en
Priority to AU2005201044A priority patent/AU2005201044B2/en
Priority to US11/982,434 priority patent/US8114851B2/en
Priority to US11/982,325 priority patent/US20080171862A1/en
Priority to US11/982,425 priority patent/US8183362B2/en
Priority to US11/982,305 priority patent/US8101742B2/en
Priority to US11/982,441 priority patent/US8114981B2/en
Priority to US11/982,345 priority patent/US8729037B2/en
Priority to US13/656,548 priority patent/US9902954B2/en
Priority to US13/656,513 priority patent/US8877726B2/en
Priority to US13/656,540 priority patent/US20130164366A1/en
Priority to US13/753,438 priority patent/US20130177631A1/en
Priority to US14/218,476 priority patent/US9133454B2/en
Priority to US14/218,489 priority patent/US9902955B2/en
Priority to US15/870,380 priority patent/US20180179526A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the invention relates to methods according to the preambles of claims 1 and 2. It also relates to a medicament and a use of double-stranded oligoribonucleotides and a vector coding therefor.
  • Such a method is known from the subsequently published WO 99/32619.
  • the known method aims at inhibiting the expression of genes in cells of invertebrates.
  • the double-stranded oligoribonucleotide has a sequence identical to the target gene with a length of at least 50 bases.
  • the length of the identical sequence is 300 to 1000 base pairs. The production cost of such an oligoribonucleotide is high.
  • the anti-sense RNA is an R ⁇ A molecule that is complementary to regions of the mRNA. Binding to these areas results in an inhibition of gene expression. This inhibition can be used in particular for the diagnosis and / or therapy of diseases, e.g. Tumor diseases or viral infections.
  • the anti-sense RNA must disadvantageously be introduced into the cell in an amount which is at least as large as the amount of the mRNA. The effectiveness of the known anti-sense methods is not particularly high.
  • No. 5,712,257 discloses a medicament which mismatched double-stranded R ⁇ A (dsR A) and biologically active mismatched fragments of dsR ⁇ A in the form of a ternary complex. xes with a surfactant.
  • the dsRNA used consists of synthetically produced single nucleic acid strands without a defined base sequence. The single strands enter into non-regular, so-called "non-Watson-Crick" base pairs, so that mismatched double strands are formed.
  • the known dsRNA is used to inhibit the multiplication of retroviruses, such as HIV. The proliferation of the virus can be inhibited if non-sequence-specific dsRNA is introduced into the cells. This leads to an induction of interferon, which is intended to inhibit virus replication. The inhibitory effect or effectiveness of this process is low.
  • dsRNA one strand of which is complementary in sections to a gene of a roundworm to be inhibited, inhibits the expression of this gene with a high effectiveness. It is believed that the particular effectiveness of the dsRNA used in roundworm cells is not based on the anti-sense principle, but possibly on catalytic
  • the object of the present invention is to eliminate the disadvantages of the prior art.
  • the most efficient method, medicament or as efficient an use as possible for the production of a medicament is to be specified, with which a particularly effective inhibition of the expression of a given target gene can be brought about.
  • This object is solved by the features of claims 1, 2, 37, 38 and 74 and 75.
  • Advantageous configurations result from claims 3 to 36, 39 to 73 and 76 to 112.
  • region I complementary to the target gene has a maximum of 49 successive nucleotide pairs.
  • an oligoribonucleotide or a vector coding therefor are provided.
  • the oligoribonucleotide has a defined nucleotide sequence at least in sections.
  • the defined section can be limited to the complementary area I.
  • it can also be the case that the double-stranded oligoribonucleotide as a whole has a defined nucleotide sequence.
  • dsRNA with a length of more than 50 nucleotide pairs induces certain cellular mechanisms in mammalian and human cells, e.g. the dsRNA-dependent protein kinase or the 2-5A system. This leads to the disappearance of the interference effect mediated by the dsRNA having a defined sequence. This blocks protein biosynthesis in the cell. This disadvantage in particular is eliminated by the present invention.
  • dsRNA or the vector can be packaged in micellar structures, preferably in liposomes.
  • the dsRNA or the vector can likewise be enclosed in viral natural capsids or in artificial capsids produced by chemical or enzymatic means or structures derived therefrom. - The aforementioned features enable the dsRNA or the vector to be introduced into predetermined target cells.
  • the dsRNA has 10 to 1000, preferably 15 to 49, base pairs.
  • the dsRNA can therefore be longer than region I which is complementary to the target gene.
  • the complementary region I can be arranged at the end or switched into the dsRNA.
  • Such a dsR ⁇ A or a vector provided for coding the same can be produced synthetically or enzymatically using conventional methods.
  • the gene to be inhibited is expediently expressed in eukaryotic cells.
  • the target gene can be selected from the following group: oncogene, cytokine gene, Id protein gene, development gene, prion gene. It can also be expressed in pathogenic organisms, preferably in plasmodia. It can be part of a, preferably human pathogenic, virus or viroid.
  • the proposed method enables the production of agents for the therapy of genetically controlled diseases, e.g. Cancer, viral diseases or Alzheimer's disease.
  • the virus or viroid can also be an animal or phytopathogenic virus or viroid.
  • the method according to the invention also allows the provision of agents for the treatment of animal or plant diseases.
  • the dsRNA is double-stranded in sections.
  • a region II which is complementary within the double-stranded structure is formed from two separate RNA single strands or from self-complementary regions of a, preferably circular, topologically closed RNA single strand.
  • the ends of the dsRNA can be modified to counteract degradation in the cell or dissociation into the single strands. Dissociation occurs particularly when using low concentrations or short chain lengths. For particularly effective inhibition of dissociation, the cohesion of the complementary region II brought about by the nucleotide pairs can be increased by at least one, preferably two, further chemical linkages.
  • a dsRNA according to the invention, the dissociation of which is reduced, has a higher stability against enzymatic and chemical degradation in the cell or in the organism.
  • the complementary region II can be formed from self-complementary regions of an RNA hairpin loop.
  • the nucleotides are in the loop area between the double-stranded
  • the chemical linkage is expediently formed by a covalent or ionic bond, a hydrogen bond, hydrophobic interactions, preferably van der Waals or stacking interactions, or by metal ion coordination. According to a particularly advantageous design feature, it can be attached to at least one, preferred be made at both ends of the complementary area II.
  • the chemical linkage is formed by means of one or more connecting groups, the connecting groups preferably being poly (oxyphosphinicooxy-1,3-propanediol) and / or polyethylene glycol chains.
  • the chemical linkage can also be formed by purine analogs used in the complementary areas II instead of purines. It is also advantageous that the chemical linkage is formed by azabenzene units introduced in the complementary regions II. It can also be formed by branched nucleotide analogs used in the complementary regions II instead of nucleotides.
  • the chemical linkage can be formed by thiophosphoryl groups attached to the ends of the double-stranded region.
  • the chemical linkage is preferably produced at the ends of the double-stranded region by triple helix bonds.
  • the chemical linkage can expediently be induced by ultraviolet light.
  • the nucleotides of the dsRNA can be modified. This counteracts activation of a protein kinase, PKR, which is dependent on double-stranded RNA in the cell.
  • PKR protein kinase
  • at least one 2 'hydroxyl group is the nucleotides of the dsRNA in the complementary region II by a chemical group, preferably a 2'-amino or a 2 'methyl group.
  • At least one nucleotide in at least one strand of the complementary region II can also be a so-called "locked nucleotide" with a sugar ring that is chemically modified, preferably by a 2'-0, 4'-C-methylene bridge.
  • several nucleotides are "locked nucleotides”.
  • the dsRNA or the vector is bound to, associated with or surrounded by at least one viral coat protein derived from a virus, derived therefrom or synthetically produced.
  • the coat protein can be derived from the polyomavirus.
  • the coat protein can contain the virus protein 1 (VP1) and / or the virus protein 2 (VP2) of the polyoma virus.
  • VP1 virus protein 1
  • VP2 virus protein 2
  • the use of such coat proteins is e.g. known from DE 196 18 797 AI, the disclosure content of which is hereby included. -
  • the aforementioned features significantly facilitate the introduction of the dsRNA or the vector into the cell.
  • a capsid or capsid-like structure is formed from the coat protein, one side faces the interior of the capsid or capsid-like structure.
  • the construct formed is particularly stable.
  • the dsRNA can be complementary to the primary or processed RNA transcript of the target gene.
  • the cell can be a vertebrate cell or a human cell.
  • At least two different dsRNAs or at least one vector coding therefor can be introduced into the cell, a strand of each dsRNA being complementary, at least in sections, to each of at least one is two different target genes. This makes it possible to simultaneously inhibit the expression of at least two different target genes.
  • PKR protein kinase
  • one of the target genes is advantageously the PKR gene. This can effectively suppress PKR activity in the cell.
  • a medicament with at least one oligoribonucleotide with a double-stranded structure is also provided for inhibiting the expression of a given target gene, one strand of the dsRNA having a region I which is at least partially complementary to the target gene.
  • dsRNA double-stranded structure
  • the inhibition is brought about at concentrations which are at least one order of magnitude lower.
  • the medicament according to the invention is highly effective. Less side effects are expected.
  • a medicament is provided with at least one vector for coding at least one oligoribonucleotide with a double-stranded structure (dsRNA) for inhibiting the expression of a given target gene, wherein one strand of the dsRNA has a region I which is at least partially complementary to the target gene .
  • dsRNA double-stranded structure
  • the complementary region I has at most 49 consecutive pairs of nucleotides. - Surprisingly, it has been shown that an efficient inhibition of the expression of the target gene can be achieved even with a length of the complementary region I of at most 49 base pairs. Corresponding oligoribonucleotides can be provided with less manufacturing effort.
  • an oligoribonucleotide with a double-stranded structure is provided for the manufacture of a medicament for inhibiting the expression of a given target gene, one strand of the dsRNA having a region I which is at least partially complementary to the target gene.
  • a dsRNA is suitable for the manufacture of a medicament for inhibiting the expression of a given gene.
  • the inhibition is brought about in comparison to the use of single-stranded oligoribonucleotides at concentrations which are lower by an order of magnitude. The use according to the invention thus enables the production of particularly effective drugs.
  • a vector for coding at least one oligoribonucleotide with a double-stranded structure is provided for the manufacture of a medicament for inhibiting the expression of a given target gene, one strand of the dsRNA having a region I which is at least partially complementary to this target gene.
  • 1 is a schematic representation of a plasmid for in vitro transcription with T7 and SP6
  • Embodiment 1 The inhibition of transcription was demonstrated by sequence homologous dsRNA in an in vitro transcription system with a core extract from human HeLa cells.
  • the DNA template for this experiment was the BamHI linearized plasmid PCMV1200.
  • the plasmid shown in Fig. 1 was constructed for use in the enzymatic synthesis of the dsRNA.
  • a polymerase chain reaction (PCR) was first carried out with the "positive control DNA" of the HeLaScribe ® Nuclear Extract in vi tro transcription kits from Promega, Madison, USA as a DNA template.
  • One of the primers used contained the sequence of an EcoRI site and the T7 RNA polymerase promoter according to the sequence listing ⁇ r. 1.
  • the other primer kept the sequence of a BamHI site and the SP6-PNA polymerase promoter according to sequence listing No. 2.
  • both primers had identical or complementary regions to the DNA template at their 3 'ends.
  • the PCR was carried out using the "aq PCR Core Kit” from Qiagen, Hilden, Germany, according to the manufacturer's instructions. In a volume of 100 ⁇ l, 1.5 mM MgCl 2 , each 200 ⁇ M dNTP, each 0.5 ⁇ M primer, 2.5 U tag DNA polymerase and approximately 100 ng “positive control DNA” were used as a template in PCR buffer used. After the initial denaturation of the template DNA by heating to 94 ° C. for 5 minutes, the amplification was carried out in 30 cycles of 60 seconds denaturation at 94 ° C., 60 seconds annealing at 5 ° C. below the calculated melting temperature of the primers and 1.5 - 2 minutes of polymerization at 72 ° C.
  • the length of the DNA fragment amplified in this way was 400 base pairs, 340 base pairs corresponding to the "positive control DNA”.
  • the PCR product was purified, hydrolyzed with ScoRI and BamHI and, after further purification, used for ligation with a pUC18 vector that was also hydrolyzed by EcoRI and BamHI. E. coli XLl-blue was transformed.
  • the plasmid obtained (pCMV5) carries a DNA fragment which is flanked at the 5 'end by the T7 and at the 3' end by the SP6 promoter.
  • the plasmid By linearizing the plasmid with BamHI, it can be used in vitro with the T7 RNA polymerase for the run-off transcription of a 340 nucleotide long single-stranded RNA shown in Sequence Listing No. 3. If the plasmid is linearized with BcoRI, it can be used for run-off transcription with the SP6-RNA polymerase, whereby the complementary strand is formed. A 23 nucleotide longer RNA was also synthesized in accordance with the method described above. This was done in sequence listing No. 4 DNA ligated via the EcoRI and BamHI sites with the pUCl ⁇ vector.
  • the plasmid pCMV1200 was constructed as a DNA template for in vitro transcription with HeLa core extract.
  • a 1191 bp EcoRI / BamHI fragment was amplified positive control DNA contained in HeLaScribe ® Nuclear Extract in vi tro transcription kit using PCR.
  • the amplified fragment comprises the 828 bp "immediate early" CMV promoter and a 363 bp transcribable DNA fragment.
  • the PCR product was ligated to the vector pGEM-T via "T-overhang" ligation. At the 5 'end of the fragment is a BamHI site.
  • the plasmid was linearized by hydrolysis with BamHI and used as a template for run-off transcription.
  • pCMV5 plasmid DNA was linearized with EcoRI or BamHI. It was used as a DNA template for in vitro transcription of the complementary RNA single strands with SP6 or T7 -PNA polymerase.
  • SP6 or T7 -PNA polymerase the "Riboprobe in vi tro Transcription" system from Promega, Madison, USA was used. According to the manufacturer's instructions, 2 ⁇ g of linearized plasmid DNA were incubated for 5 to 6 hours at 37 ° C. in 100 ⁇ l of transcription buffer and 40 U of T7 or SP6-KNA polymerase.
  • the DNA template was then degraded by adding 2.5 ⁇ l of RNase-free D ⁇ ase RQ1 and incubating at 37 ° C. for 30 minutes.
  • the transcription batch was made up to 300 ⁇ l with H 2 O and purified by phenol extraction.
  • the RNA was precipitated by adding 150 ⁇ l of 7 M ammonium acetate and 1125 ⁇ l of ethanol and stored at -65 ° C. until hybridization.
  • the dsRNA preparations contained single-stranded RNA (ssRNA) as contamination.
  • ssRNA single-stranded RNA
  • the batches were treated after the hybridization with the single-strand-specific ribonucleases RNase A from bovine pancreas and RNase Tl from Aspergillus oryzae.
  • RNase A is a pyrimidine-specific endoribonuclease.
  • RNase Tl is an endoribonuclease that preferentially cuts on the 3 'side of guanosines.
  • dsRNA is not a substrate for these ribonucleases.
  • the dried pellet was taken up in 15 ⁇ l TE buffer, pH 6.5 and subjected to a native polyacrylamide gel electrophoresis on an 8% gel. The acrylamide gel was then Colored in an ethidium bromide solution and rinsed in a water bath. 2 shows the RNA visualized on a UV transilluminator.
  • the sense RNA applied on lane 1 and the antiseptic RNA applied on lane 2 showed a different running behavior under the chosen conditions than the dsRNA of the hybridization approach applied on lane 3.
  • the RNase-treated sense or antisense RNA applied to lanes 4 and 5 produced no visible band. This shows that the single-stranded RNAs have been completely broken down.
  • the RNase-treated dsRNA of the hybridization approach applied on lane 6 is resistant to the RNase treatment.
  • the band which migrates faster in the native gel compared to the dsRNA applied on lane 3 results from dsRNA which is free of ssRNA. In addition to the dominant main band, weaker, more rapidly migrating bands appear after RNase treatment.
  • PCMV1200 contained, homologous to "positive control DNA"
  • dsRNA-CMV5 determined.
  • dsRNA-YFP non-sequence homologous dsRNA
  • YFP yellow fluorescent protein
  • the transcription was carried out using the HeLa core extract, which contains all the necessary proteins for a trans contains script.
  • radioactively labeled transcript was obtained.
  • the [»- 32 P] rGTP used had a specific activity of 400 Ci / mmol, 10 Ci / ml. 3 mM MgCl 2 , 400 ⁇ M rATP, rCTP, rUTP, 16 ⁇ M rGTP, 0.4 ⁇ M [.- 32 P] rGTP and, depending on the experiment, 1 fmol linearized plasmid DNA and various amounts of dsRNA in transcription buffer were used per batch .
  • RNA transcripts formed in the denaturing sample buffer during the transcription with HeLa core extract were heated to 90 ° C.
  • the decrease in the transcript when sequence-specific dsRNA is added can therefore be clearly attributed to the dsRNA itself.
  • the reduction in the amount of transcript of a gene in the presence of dsRNA in a human transcription system indicates an inhibition of the expression of the corresponding gene. This effect is due to a novel mechanism caused by the dsRNA.
  • the murine fibroblast cell line NIH3T3, ATCC CRL-1658 served as the test system for these in vivo experiments.
  • the YFP gene was introduced into the cell nuclei using microinjection. The expression of the YFP was examined under the influence of sequence homologous dsRNA transfected simultaneously. This dsRNA YFP is homologous to the 5 'region of the YFP gene over a length of 315 bp.
  • the nucleotide sequence of a strand of the dsRNA-YFP is shown in Sequence Listing No. 5. The evaluation under the fluorescence microscope was carried out 3 hours after injection using the green-yellow fluorescence of the YFP formed.
  • a plasmid was constructed according to the same principle as described in embodiment example 1.
  • the desired gene fragment was amplified using PCR using the primer Eco__T7_YFP according to sequence listing No. 6 and Bam_SP6_YFP according to sequence listing No. 7 and used for the production of the dsRNA analogously to the description above.
  • the dsRNA-YFP obtained is identical to the dsRNA used in embodiment 1 as a non-sequence-specific control.
  • a dsRNA (L-dsRNA) chemically linked to the 5 'end of the complementary RNA was produced at the 3' end of the RNA according to sequence listing No. 8 via a Cl8 linker group. Synthones modified with disulfide bridges were used for this.
  • the 3'-terminal synthon is bound to the solid support via the 3 'carbon with an aliphatic linker group via a disulfide bridge.
  • the 5'-trityl protective group is bound via a further aliphatic linker and a disulfide bridge.
  • the resulting thiol groups come into spatial proximity to one another.
  • the single strands are linked by oxidation via their aliphatic linkers and a disulfide bridge. This is followed by cleaning using HPLC.
  • the cells were incubated in DMEM with 4.5 g / 1 glucose, 10% fetal bovine serum under 7.5% CO 2 atmosphere at 37 ° C. in culture dishes and passaged before reaching confluence.
  • the cells were detached using trypsin / EDTA. To prepare for microinjection, the cells were transferred to petri dishes and incubated further until microcolonies were formed.
  • the culture dishes were removed from the incubator for about 10 minutes for microinjection. It was built in approx. 50 cell nuclei per batch are individually injected within a marked area using the AIS microinjection system from Carl Zeiss, Göttingen, Germany. The cells were then incubated for a further three hours. Borosilicate glass capillaries from Hilgenberg GmbH, Malsfeld, Germany with a tip diameter of less than 0.5 ⁇ m were prepared for microinjection. The microinjection was carried out using a micromanipulator from Narishige Scientific Instrument Lab., Tokyo, Japan. The injection time was 0.8 seconds, the pressure was approximately 100 hPa.
  • the plasmid pCDNA-YFP was used for the transfection, which contains an approximately 800 bp BamHI / EcoRI fragment with the gene of the YFP in the vector pcDNA3.
  • the samples injected into the cell nuclei contained 0.01 ⁇ g / ⁇ l pCDNA-YFP and Texas red coupled to dextran-70000 in 14 mM NaCl, 3 M KC1, 10 mM KP0 4 , pH 7.5.
  • FIG. 4 a shows the result for NIH3T3 cells.
  • sense-YFP-ssRNA in FIG. 4 b antisense-YFP-ssRNA
  • antisense-YFP-ssRNA in FIG. 4 c dsRNA-YFP
  • no RNA and in FIG. 4 e L -dsRNA was injected.
  • the left field shows the fluorescence of cells that were excited with 568 nm.
  • the fluorescence of the same cells when excited with 488 nm can be seen on the right.
  • the Texas red fluorescence of all the cells shown shows that the injection solution was successfully applied to the cell nuclei and that the cells hit were still alive after three hours. Dead cells no longer showed Texas red fluorescence.
  • FIGS. 4 a and 4 b show that the expression of the YFP was not visibly inhibited when the single-stranded RNA was injected into the cell nuclei.
  • the right field of FIG. 4 c shows cells whose YFP fluorescence was no longer detectable after injection of dsRNA-YFP.
  • 4 d shows as a control cells into which no RNA had been injected.
  • the cell shown in FIG. 4 e shows, by injection of the L-dsRNA, which has regions homologous to the YFP gene, an undetectable YFP fluorescence. This result proves that shorter dsRNAs can also be used for the specific inhibition of gene expression in mammals if the double strands are stabilized by chemically linking the single strands.
  • Azhayeva E., Azhayev, A., Auriola, S., Tengvall, U., Urtti,

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Hospice & Palliative Care (AREA)
  • Psychiatry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
PCT/DE2000/000244 1999-01-30 2000-01-29 Verfahren und medikament zur hemmung der expression eines vorgegebenen gens Ceased WO2000044895A1 (de)

Priority Applications (28)

Application Number Priority Date Filing Date Title
JP2000596137A JP2003502012A (ja) 1999-01-30 2000-01-29 特定遺伝子の発現を抑制するための方法および薬剤
CA002359180A CA2359180C (en) 1999-01-30 2000-01-29 Method and medicament for inhibiting the expression of a given gene
DE50000414T DE50000414D1 (de) 1999-01-30 2000-01-29 Verfahren und medikament zur hemmung der expression eines vorgegebenen gens
DK05002454T DK1550719T3 (da) 1999-01-30 2000-01-29 Dobbeltstrenget RNA (dsRNA) til inhibering af ekspressionen af et defineret gen
AT00910510T ATE222953T1 (de) 1999-01-30 2000-01-29 Verfahren und medikament zur hemmung der expression eines vorgegebenen gens
DE10080167T DE10080167B4 (de) 1999-01-30 2000-01-29 Medikament zur Hemmung der Expression eines vorgegebenen Gens
AU2000032713A AU778474B2 (en) 1999-01-30 2000-01-29 Method and medicament for inhibiting the expression of a defined gene
EP02003683.6A EP1214945B2 (de) 1999-01-30 2000-01-29 Verfahren und medikament zur hemmung der expression eines vorgegebenen gens
EP00910510A EP1144623B9 (de) 1999-01-30 2000-01-29 Verfahren und medikament zur hemmung der expression eines vorgegebenen gens
US10/382,395 US8101584B2 (en) 1999-01-30 2003-03-06 Method and medicament for inhibiting the expression of a given gene
US10/383,099 US8119608B2 (en) 1999-01-30 2003-03-06 Method and medicament for inhibiting the expression of a given gene
US10/382,768 US8168776B2 (en) 1999-01-30 2003-03-06 Method for making a 21 nucleotide double stranded RNA chemically linked at one end
US10/384,339 US7829693B2 (en) 1999-11-24 2003-03-07 Compositions and methods for inhibiting expression of a target gene
US10/612,179 US8202980B2 (en) 1999-01-30 2003-07-02 Method and medicament for inhibiting the expression of a given gene
AU2005201044A AU2005201044B2 (en) 1999-01-30 2005-03-09 Method and medicament for inhibiting the expression of a defined gene
US11/982,434 US8114851B2 (en) 1999-01-30 2007-10-31 Method and medicament for inhibiting the expression of a given gene
US11/982,305 US8101742B2 (en) 1999-01-30 2007-10-31 Method and medicament for inhibiting the expression of a given gene
US11/982,345 US8729037B2 (en) 1999-01-30 2007-10-31 Method and medicament for inhibiting the expression of a given gene
US11/982,325 US20080171862A1 (en) 1999-01-30 2007-10-31 Method and medicament for inhibiting the expression of a given gene
US11/982,441 US8114981B2 (en) 1999-01-30 2007-10-31 Method and medicament for inhibiting the expression of a given gene
US11/982,425 US8183362B2 (en) 1999-01-30 2007-10-31 Method and medicament for inhibiting the expression of a given gene
US13/656,540 US20130164366A1 (en) 1999-01-30 2012-10-19 Method and medicament for inhibiting the expression of a given gene
US13/656,548 US9902954B2 (en) 1999-01-30 2012-10-19 Method and medicament for inhibiting the expression of a given gene
US13/656,513 US8877726B2 (en) 1999-01-30 2012-10-19 Method and medicament for inhibiting the expression of a given gene
US13/753,438 US20130177631A1 (en) 1999-01-30 2013-01-29 Method and medicament for inhibiting the expression of a given gene
US14/218,476 US9133454B2 (en) 1999-01-30 2014-03-18 Method and medicament for inhibiting the expression of a given gene
US14/218,489 US9902955B2 (en) 1999-01-30 2014-03-18 Method and medicament for inhibiting the expression of a given gene
US15/870,380 US20180179526A1 (en) 1999-01-30 2018-01-12 Method and Medicament For Inhibiting The Expression of A Given Gene

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19903713 1999-01-30
DE19903713.2 1999-01-30
DE19956568A DE19956568A1 (de) 1999-01-30 1999-11-24 Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
DE19956568.6 1999-11-24

Related Child Applications (8)

Application Number Title Priority Date Filing Date
US09889802 A-371-Of-International 2000-01-29
US10/382,395 Division US8101584B2 (en) 1999-01-30 2003-03-06 Method and medicament for inhibiting the expression of a given gene
US10/382,768 Division US8168776B2 (en) 1999-01-30 2003-03-06 Method for making a 21 nucleotide double stranded RNA chemically linked at one end
US10/382,768 Continuation US8168776B2 (en) 1999-01-30 2003-03-06 Method for making a 21 nucleotide double stranded RNA chemically linked at one end
US10/383,099 Division US8119608B2 (en) 1999-01-30 2003-03-06 Method and medicament for inhibiting the expression of a given gene
US10/384,339 Continuation-In-Part US7829693B2 (en) 1999-11-24 2003-03-07 Compositions and methods for inhibiting expression of a target gene
US10/612,179 Division US8202980B2 (en) 1999-01-30 2003-07-02 Method and medicament for inhibiting the expression of a given gene
US10/612,179 Continuation US8202980B2 (en) 1999-01-30 2003-07-02 Method and medicament for inhibiting the expression of a given gene

Publications (1)

Publication Number Publication Date
WO2000044895A1 true WO2000044895A1 (de) 2000-08-03

Family

ID=26051592

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/000244 Ceased WO2000044895A1 (de) 1999-01-30 2000-01-29 Verfahren und medikament zur hemmung der expression eines vorgegebenen gens

Country Status (13)

Country Link
US (17) US8119608B2 (enExample)
EP (6) EP1214945B2 (enExample)
JP (14) JP2003502012A (enExample)
AT (3) ATE222953T1 (enExample)
AU (2) AU778474B2 (enExample)
CA (1) CA2359180C (enExample)
CY (3) CY1108896T1 (enExample)
DE (7) DE19956568A1 (enExample)
DK (4) DK1214945T4 (enExample)
ES (5) ES2182791T3 (enExample)
PT (4) PT2363479T (enExample)
WO (1) WO2000044895A1 (enExample)
ZA (1) ZA200105909B (enExample)

Cited By (488)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10100586C1 (de) * 2001-01-09 2002-04-11 Ribopharma Ag Verfahren zur Hemmung der Expression eines Ziegens
DE10100587C1 (de) * 2001-01-09 2002-11-21 Ribopharma Ag Verfahren zur Hemmung der Expression eines Zielgens
WO2002059257A3 (en) * 2000-10-31 2002-12-19 Commw Scient Ind Res Org Method and means for producing barley yellow dwarf virus resistant cereal plants
WO2003033700A1 (de) * 2001-10-12 2003-04-24 Ribopharma Ag Verfahren zur hemmung der replikation von viren
WO2003035868A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Medikament zur erhöhung der wirksamkeit eines rezeptor-vermittelt apoptose in tumorzellen auslösenden arzeimittels
WO2003035876A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Verwendung einer doppelsträngigen ribonukleinsäure zur behandlung einer infektion mit einem (+)-strang-rna-virus
WO2003035869A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Verwendung einer doppelsträngigen ribonukleinsäure zur gezielten hemmung der expression eines vorgegebenen zielgens
WO2003035082A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Medikament zur hemmung der expression eines zielgens
WO2002055693A3 (de) * 2001-01-09 2003-07-17 Ribopharma Ag Verfahren zur hemmung der expression eines zielgens
WO2003008573A3 (en) * 2001-07-17 2003-07-17 Anne Josephine Milner Silencing of gene expression by sirna
JP2003529374A (ja) * 2000-03-30 2003-10-07 ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ Ｒｎａ干渉のｒｎａ配列特異的メディエータ
WO2003072705A3 (en) * 2002-02-20 2003-11-06 Sirna Therapeutics Inc Rna interference mediated inhibition of cyclin d1 gene expression using short interfering nucleic acid (sina)
WO2002068635A3 (en) * 2001-02-28 2003-12-04 Novartis Forschungsstiftung Methods of inhibiting expression of a target gene in mammalian cells
EP1389637A1 (en) * 2002-08-05 2004-02-18 atugen AG Novel forms of interfering RNA molecules
WO2004018687A2 (en) 2002-08-07 2004-03-04 Basf Plant Science Gmbh Nucleic acid sequences encoding proteins associated with abiotic stress response
WO2004022075A1 (en) 2002-09-04 2004-03-18 Novartis Ag Treatment of neurological disorders by dsrna adminitration
WO2004015107A3 (en) * 2002-08-05 2004-04-29 Atugen Ag Further novel forms of interfering rna molecules
WO2004044136A2 (en) 2002-11-05 2004-05-27 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2’-modified nucleosides for use in gene modulation
WO2004044138A2 (en) 2002-11-05 2004-05-27 Isis Pharmaceuticals, Inc. Chimeric oligomeric compounds and their use in gene modulation
JP2004173512A (ja) * 2002-11-22 2004-06-24 Takashi Morita ＲＮＡｉによる新規治療法および治療剤
WO2003062423A3 (de) * 2002-01-22 2004-07-22 Ribopharma Ag Verfahren zur hemmung der expression eines durch eine chromosomen-aberration entstandenen zielgens
WO2004091515A2 (en) 2003-04-09 2004-10-28 Alnylam Pharmaceuticals, Inc. iRNA CONJUGATES
WO2004094345A2 (en) 2003-04-17 2004-11-04 Alnylam Pharmaceuticals Inc. Protected monomers
WO2004092383A3 (en) * 2003-04-15 2005-02-10 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF SEVERE ACUTE RESPIRATORY SYNDROME (SARS) VIRUS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
EP1442143A4 (en) * 2002-02-20 2005-02-16 Sirna Therapeutics Inc INHIBITION OF BCL2 GENE EXPRESSION MEDIATED BY RNA INTERFERENCE USING siNA (SHORT INTERFERING NUCLEIC ACID)
WO2005014782A2 (en) 2003-06-13 2005-02-17 Alnylam Europe Ag., Double-stranded ribonucleic acid with increased effectiveness in an organism
EP1472269A4 (en) * 2002-02-20 2005-03-23 Sirna Therapeutics Inc RNA INTERFERENCE-INDUCED INHIBITION OF EXPRESSION OF INTERLEUKIN GENE USING SHORT INTERFERENT NUCLEIC ACIDS (SINA)
WO2005019453A3 (en) * 2001-05-18 2005-06-23 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING CHEMICALLY MODIFIED SHORT INTERFERING NUCLEIC ACID (siNA)
EP1521768A4 (en) * 2002-02-20 2005-07-06 Sirna Therapeutics Inc INHIBITION OF THE VASCULAR ENDOTHELIC GROWTH FACTOR AND THE EXPRESSION OF THE GENE TO THE RECEPTOR OF THE VASCULAR ENDOTHELIC GROWTH FACTOR USING SINA (SHORT INTERFERING NUCLEIC ACID) THROUGH RNA INTERFERENCE
WO2005066371A2 (en) 2003-12-31 2005-07-21 The Penn State Research Foundation Methods for predicting and overcoming resistance to chemotherapy in ovarian cancer and for predicting colon cancer occurrence
WO2005003350A3 (en) * 2003-06-27 2005-08-04 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED TREATMENT OF ALZHEIMER’S DISEASE USING SHORT INTERFERING NUCLEIC ACID (siNA)
EP1476457A4 (en) * 2002-02-20 2005-08-31 Ribozyme Pharm Inc Inhibition of the expression of the protein-tyrosine phosphatase-1B gene (PTP-1B) mediated by RNA interference, using SiNA (short interferon nucleotide acide)
WO2005045037A3 (en) * 2003-10-23 2005-11-03 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF 5-ALPHA REDUCTASE AND ANDROGEN RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
EP1501853A4 (en) * 2002-02-20 2005-11-16 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION OF AN EPIDERMAL GROWTH FACTOR RECEPTOR USING SHORT INTERFERING NUCLEIC ACIDS
WO2005007859A3 (en) * 2003-07-11 2005-12-01 Sirna Therapeutics Inc Rna interference mediated inhibition of acetyl-coa carboxylase gene expression using short interfering nucleic acid (sina)
WO2005118787A2 (en) 2004-05-10 2005-12-15 The University Of North Carolina At Chapel Hill Method of screening candidate compounds for susceptibility to biliary excretion
WO2005014811A3 (en) * 2003-08-08 2005-12-22 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF XIAP GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
EP1612266A2 (en) 2004-06-10 2006-01-04 National Taiwan University Prostaglandin reductase
WO2004097020A3 (en) * 2003-04-25 2006-01-05 Sirna Therapeutics Inc Rna interference mediated inhibition of map kinase gene expression
JP2006501808A (ja) * 2002-02-01 2006-01-19 セクイター インコーポレイテッド 増強された効力を有するオリゴヌクレオチド組成物
EP1499631A4 (en) * 2002-02-20 2006-01-25 Sirna Therapeutics Inc RNA INTERFERENCE-INDUCED INHIBITION OF GENE EXPRESSION OF TGF-BETA RECEPTOR AND TGF-BETA USING SMALL INTERFERING NUCLEIC ACID (SINA)
EP1478730A4 (en) * 2002-02-20 2006-01-25 Sirna Therapeutics Inc INTERFERENCE RNA-INDUCED INHIBITION OF THE GENE EXPRESSION OF SUPERFAMILY TFN AND TFN RECEPTOR SUPERFAMILY USING SHORT INTERFERENCE NUCLEIC ACID (SINA)
WO2006009575A1 (en) 2004-06-22 2006-01-26 The Board Of Trustees Of The University Of Illinois METHODS OF INHIBITING TUMOR CELL PROLIFERATION WITH FOXM1 siRNA
WO2006017673A2 (en) 2004-08-03 2006-02-16 Biogen Idec Ma Inc. Taj in neuronal function
JP2006505280A (ja) * 2002-11-07 2006-02-16 チャン・ルン−ジ 改変樹状細胞
WO2006020768A2 (en) 2004-08-10 2006-02-23 Alnylam Pharmaceuticals, Inc. Chemically modified oligonucleotides
WO2006017932A1 (en) 2004-08-18 2006-02-23 Genesense Technologies Inc. Small interfering rna molecules against ribonucleotide reductase and uses thereof
WO2006032707A2 (en) 2004-09-24 2006-03-30 Basf Plant Science Gmbh Plant cells and plants with increased tolerance to environmental stress
EP1645633A2 (en) 2004-10-05 2006-04-12 SunGene GmbH Constitutive expression cassettes for regulation of plant expression
EP1655364A2 (en) 2004-11-05 2006-05-10 BASF Plant Science GmbH Expression cassettes for seed-preferential expression in plants
EP1662000A2 (en) 2004-11-25 2006-05-31 SunGene GmbH Expression cassettes for guard cell-preferential expression in plants
US7056704B2 (en) 2000-12-01 2006-06-06 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
EP1666599A2 (en) 2004-12-04 2006-06-07 SunGene GmbH Expression cassettes for mesophyll- and/or epidermis-preferential expression in plants
EP1669455A2 (en) 2004-12-08 2006-06-14 SunGene GmbH Expression cassettes for vascular tissue-preferential expression in plants
EP1669456A2 (en) 2004-12-11 2006-06-14 SunGene GmbH Expression cassettes for meristem-preferential expression in plants
US7067722B2 (en) 1999-08-26 2006-06-27 Monsanto Technology Llc Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
WO2006069610A2 (en) 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
WO2006089950A2 (en) 2005-02-26 2006-08-31 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
US7122361B2 (en) 2002-10-10 2006-10-17 Wyeth Compositions employing a novel human kinase
WO2006120197A2 (en) 2005-05-10 2006-11-16 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
EP1723958A2 (fr) 2005-05-19 2006-11-22 L'oreal Vectorisation de dsRNA par des particules cationiques et utilisation topique
US7148336B2 (en) 1999-08-26 2006-12-12 Calgene Llc Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acid levels
US7166771B2 (en) 2002-06-21 2007-01-23 Monsanto Technology Llc Coordinated decrease and increase of gene expression of more than one gene using transgenic constructs
US7176304B2 (en) 2002-02-20 2007-02-13 Mcswiggen James RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US7208306B2 (en) 2002-10-24 2007-04-24 Wyeth Compositions employing a novel human protein phosphatase
WO2007054441A2 (de) 2005-11-08 2007-05-18 Basf Plant Science Gmbh Verwendung von armadillo-repeat (arm1)-polynukleotiden zum erreichen einer pathogenresistenz in pflanzen
EP1710307A3 (en) * 2002-02-20 2007-06-06 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
EP1741781A3 (en) * 2002-02-20 2007-06-06 Sirna Therapeutics, Inc. RNA interference mediated treatment of Alzheimer's disease using short interfering nucleic acid (siNA)
WO2007076904A1 (en) 2005-12-30 2007-07-12 Evonik Röhm Gmbh Peptides useful as cell-penetrating peptides
WO2007087815A2 (en) 2004-12-17 2007-08-09 Metanomics Gmbh Process for the control of production of fine chemicals
US20070191294A1 (en) * 2003-03-21 2007-08-16 Santaris Pharma A/S Short interfering rna (sirna) analogues
WO2007115168A2 (en) 2006-03-31 2007-10-11 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of eg5 gene
US7297525B2 (en) 2002-11-27 2007-11-20 Wyeth Composition employing a novel human kinase
US7320965B2 (en) 2005-10-28 2008-01-22 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Huntingtin gene
WO2008036933A2 (en) 2006-09-21 2008-03-27 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the hamp gene
US7399586B2 (en) 2002-05-23 2008-07-15 Ceptyr, Inc. Modulation of biological signal transduction by RNA interference
WO2008092153A2 (en) 2007-01-26 2008-07-31 University Of Louisville Research Foundation, Inc. Modification of exosomal components for use as a vaccine
WO2008099013A1 (en) 2007-02-16 2008-08-21 Basf Plant Science Gmbh Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants
US7417072B2 (en) 2005-05-12 2008-08-26 Wisconsin Alumni Research Foundation Blockade of Pin1 prevents cytokine production by activated immune cells
US7423142B2 (en) 2001-01-09 2008-09-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
US7456335B2 (en) 2001-09-03 2008-11-25 Basf Plant Science Gmbh Nucleic acid sequences and their use in methods for achieving pathogen resistance in plants
JP2008283975A (ja) * 2002-02-20 2008-11-27 Sirna Therapeutics Inc 短干渉核酸（ｓｉＮＡ）を用いるＣ型肝炎ウイルス（ＨＣＶ）遺伝子発現のＲＮＡ干渉媒介性阻害
US7491805B2 (en) 2001-05-18 2009-02-17 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
EP2036984A2 (de) 2002-07-26 2009-03-18 BASF Plant Science GmbH Revertierung der negativ-selektiven Wirkung von negativen Markerproteinen als Selektionsverfahren
EP2045327A2 (en) 2005-03-08 2009-04-08 BASF Plant Science GmbH Expression enhancing intron sequences
WO2009044392A2 (en) 2007-10-03 2009-04-09 Quark Pharmaceuticals, Inc. Novel sirna structures
US7517864B2 (en) 2001-05-18 2009-04-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
EP2055310A1 (en) 2002-08-14 2009-05-06 Silence Therapeutics Aktiengesellschaft Further use of protein kinase N beta
US7531718B2 (en) 1999-08-26 2009-05-12 Monsanto Technology, L.L.C. Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
EP1572067A4 (en) * 2001-05-18 2009-05-13 Sirna Therapeutics Inc CONJUGATES AND COMPOSITIONS FOR CELLULAR RELEASE
EP2058408A2 (en) 2003-02-14 2009-05-13 Sagres Discovery, Inc. Therapeutic GPCR targets in cancer
US7538095B2 (en) 1997-12-23 2009-05-26 The Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
EP2062591A1 (en) 2005-04-07 2009-05-27 Novartis Vaccines and Diagnostics, Inc. CACNA1E in cancer diagnosis detection and treatment
EP2072619A1 (en) 2002-10-18 2009-06-24 Silence Therapeutics Aktiengesellschaft Factor involved in metastasis and uses thereof
US7566813B2 (en) 2002-03-21 2009-07-28 Monsanto Technology, L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
EP2083088A2 (en) 2005-04-07 2009-07-29 Novartis Vaccines and Diagnostics, Inc. Cancer-related genes
EP2090662A2 (en) 2006-04-05 2009-08-19 Metanomics GmbH Process for the production of a fine chemical
US7582745B2 (en) 2005-11-04 2009-09-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Nav1.8 gene
US7592324B2 (en) 2005-02-01 2009-09-22 Alcon, Inc. RNAi-mediated inhibition of ocular targets
US7595306B2 (en) 2003-06-09 2009-09-29 Alnylam Pharmaceuticals Inc Method of treating neurodegenerative disease
WO2009123764A2 (en) 2008-04-04 2009-10-08 Calando Pharmaceuticals, Inc. Compositions and use of epas1 inhibitors
US7601888B2 (en) 2002-03-21 2009-10-13 Monsanto Technology L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
US7605251B2 (en) 2006-05-11 2009-10-20 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
US7611839B2 (en) 2002-11-21 2009-11-03 Wyeth Methods for diagnosing RCC and other solid tumors
US7622454B2 (en) 2004-12-23 2009-11-24 Alcon, Inc. RNAi inhibition of CTGF for treatment of ocular disorders
US7626015B2 (en) 2006-06-09 2009-12-01 Quark Pharmaceuticals, Inc. Therapeutic uses of inhibitors of RTP801L
WO2009147684A2 (en) 2008-06-06 2009-12-10 Quark Pharmaceuticals, Inc. Compositions and methods for treatment of ear disorders
US7655790B2 (en) 2002-07-12 2010-02-02 Sirna Therapeutics, Inc. Deprotection and purification of oligonucleotides and their derivatives
US7655402B2 (en) 2004-02-06 2010-02-02 Wyeth Llc Diagnoses and therapeutics for cancer
US7659389B2 (en) 2001-05-18 2010-02-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of MYC and/or MYB gene expression using short interfering nucleic acid (siNA)
US7659390B2 (en) 2002-02-20 2010-02-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of muscarinic colinergic receptor gene expression using short interfering nucleic acid (siNA)
US7662952B2 (en) 2002-02-20 2010-02-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acid (siNA)
US7662951B2 (en) 2000-08-30 2010-02-16 Sirna Therapeutics, Inc. RNA interference mediated treatment of Alzheimer's disease using short interfering nucleic acid (siNA)
US7667029B2 (en) 2002-02-20 2010-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of checkpoint kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA)
US7667030B2 (en) 2002-02-20 2010-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of matrix metalloproteinase 13 (MMP13) gene expression using short interfering nucleic acid (siNA)
EP2159289A2 (en) 2005-06-23 2010-03-03 BASF Plant Science GmbH Improved methods for the production of stably transformed plants
US7678897B2 (en) 2002-02-20 2010-03-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of platelet-derived endothelial cell growth factor (ECGF1) gene expression using short interfering nucleic acid (siNA)
EP2163635A1 (en) 2004-08-02 2010-03-17 BASF Plant Science GmbH Method for isolation of transcription termination sequences
US7691995B2 (en) 2001-07-12 2010-04-06 University Of Massachusetts In vivo production of small interfering RNAS that mediate gene silencing
US7691999B2 (en) 2002-02-20 2010-04-06 Sirna Therapeutics, Inc. RNA interference mediated inhibition of NOGO and NOGO receptor gene expression using short interfering nucleic acid (siNA)
US7691824B2 (en) 2006-04-28 2010-04-06 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a gene from the JC virus
US7696345B2 (en) 2002-11-05 2010-04-13 Isis Pharmaceuticals, Inc. Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
US7700760B2 (en) 2002-02-20 2010-04-20 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular cell adhesion molecule (VCAM) gene expression using short interfering nucleic acid (siNA)
DE112008001453T5 (de) 2007-05-22 2010-04-29 Basf Plant Science Gmbh Pflanzenzellen und Pflanzen mit erhöhter Toleranz und/oder Resistenz gegenüber Umweltstress und erhöhter Biomasseproduktion-KO
WO2010054406A1 (en) 2008-11-10 2010-05-14 Alnylam Pharmaceuticals, Inc. Novel lipids and compositions for the delivery of therapeutics
EP2186903A2 (en) 2005-02-09 2010-05-19 BASF Plant Science GmbH Expression cassettes for regulation of expression in monocotyledonous plants
EP2192200A1 (en) 2006-05-22 2010-06-02 Alnylam Pharmaceuticals, Inc Compositions and methods for inhibiting expression of IKK-B gene
US7732417B2 (en) 2000-03-16 2010-06-08 Cold Spring Harbor Laboratory Methods and compositions for RNA interference using recombinant Dicer and Argonaut
US7732658B2 (en) 2002-07-25 2010-06-08 Dana Farber Cancer Institute, Inc. Composition and method for imaging cells
US7737266B2 (en) 2006-09-18 2010-06-15 Board Of Regents, The University Of Texas System RNAi modulation of SCAP and therapeutics uses thereof
WO2010068816A1 (en) 2008-12-10 2010-06-17 Alnylam Pharmaceuticals, Inc. Gnaq targeted dsrna compositions and methods for inhibiting expression
US7741299B2 (en) 2004-08-16 2010-06-22 Quark Pharmaceuticals, Inc. Therapeutic uses of inhibitors of RTP801
US7745418B2 (en) 2001-10-12 2010-06-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting viral replication
US7745608B2 (en) 2003-04-17 2010-06-29 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
EP2202314A1 (en) 2007-01-15 2010-06-30 BASF Plant Science GmbH Use of subtilisin (RNR9) polynucleotides for achieving a pathogen resistance in plants
WO2010074783A1 (en) 2008-12-23 2010-07-01 The Trustees Of Columbia University In The City Of New York Phosphodiesterase inhibitors and uses thereof
EP2204376A2 (en) 2004-07-20 2010-07-07 Sagres Discovery, Inc. Novel therapeutic targets in cancer
WO2010080452A2 (en) 2008-12-18 2010-07-15 Quark Pharmaceuticals, Inc. siRNA COMPOUNDS AND METHODS OF USE THEREOF
US7767802B2 (en) 2001-01-09 2010-08-03 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
EP2216407A2 (en) 2003-03-07 2010-08-11 Alnylam Pharmaceuticals, Inc. Therapeutic Compositions
US7781393B2 (en) 2004-02-25 2010-08-24 Dana-Farber Cancer Institute, Inc. Methods for inhibiting tumor cell growth
WO2010099341A1 (en) 2009-02-26 2010-09-02 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of mig-12 gene
WO2010101951A1 (en) 2009-03-02 2010-09-10 Alnylam Pharmaceuticals, Inc. Nucleic acid chemical modifications
US7795422B2 (en) 2002-02-20 2010-09-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of hypoxia inducible factor 1 (HIF1) gene expression using short interfering nucleic acid (siNA)
WO2010105209A1 (en) 2009-03-12 2010-09-16 Alnylam Pharmaceuticals, Inc. LIPID FORMULATED COMPOSITIONS AND METHODS FOR INHIBITING EXPRESSION OF Eg5 AND VEGF GENES
WO2010107955A2 (en) 2009-03-19 2010-09-23 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF BTB AND CNC HOMOLOGY 1, BASIC LEUCINE ZIPPER TRANSCRIPTION FACTOR 1 (BACH 1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) SEQUENCE LISTING
WO2010107958A1 (en) 2009-03-19 2010-09-23 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION 6 (STAT6) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010107957A2 (en) 2009-03-19 2010-09-23 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF GATA BINDING PROTEIN 3 (GATA3) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010107952A2 (en) 2009-03-19 2010-09-23 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF CONNECTIVE TISSUE GROWTH FACTOR (CTGF) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010111471A2 (en) 2009-03-27 2010-09-30 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION 1 (STAT1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010111468A2 (en) 2009-03-27 2010-09-30 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF THE NERVE GROWTH FACTOR BETA CHAIN (NGFß) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (SINA)
WO2010111490A2 (en) 2009-03-27 2010-09-30 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF THE THYMIC STROMAL LYMPHOPOIETIN (TSLP) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010111497A2 (en) 2009-03-27 2010-09-30 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF THE INTERCELLULAR ADHESION MOLECULE 1 (ICAM-1)GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010111464A1 (en) 2009-03-27 2010-09-30 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF APOPTOSIS SIGNAL-REGULATING KINASE 1 (ASK1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US7807820B2 (en) 2002-11-14 2010-10-05 Dharmacon, Inc. siRNA targeting beta secretase (BACE)
US7812150B2 (en) 2006-05-19 2010-10-12 Alnylam Pharmaceuticals, Inc. RNAi modulation of Aha and therapeutic uses thereof
US7812002B2 (en) 2007-03-21 2010-10-12 Quark Pharmaceuticals, Inc. Oligoribonucleotide inhibitors of NRF2 and methods of use thereof for treatment of cancer
WO2010118243A2 (en) 2009-04-08 2010-10-14 Genentech, Inc. Use of il-27 antagonists to treat lupus
WO2010122110A1 (en) 2009-04-22 2010-10-28 Basf Plant Science Company Gmbh Whole seed specific promoter
US7825099B2 (en) 2006-01-20 2010-11-02 Quark Pharmaceuticals, Inc. Treatment or prevention of oto-pathologies by inhibition of pro-apoptotic genes
DE112008003433T5 (de) 2007-12-21 2010-11-04 Basf Plant Science Gmbh Pflanzen mit erhöhtem Ertrag (KO NUE)
US7829693B2 (en) 1999-11-24 2010-11-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US7833992B2 (en) 2001-05-18 2010-11-16 Merck Sharpe & Dohme Conjugates and compositions for cellular delivery
US7842674B2 (en) 2004-09-28 2010-11-30 Quark Pharmaceuticals Inc. Methods of treatment of acute renal failure
EP2258858A1 (en) 2009-06-05 2010-12-08 Universitätsklinikum Freiburg Transgenic LSD1 animal model for cancer
US7851615B2 (en) 2003-04-17 2010-12-14 Alnylam Pharmaceuticals, Inc. Lipophilic conjugated iRNA agents
WO2010144740A1 (en) 2009-06-10 2010-12-16 Alnylam Pharmaceuticals, Inc. Improved lipid formulation
US7858625B2 (en) 2001-05-18 2010-12-28 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US7858769B2 (en) 2004-02-10 2010-12-28 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using multifunctional short interfering nucleic acid (multifunctional siNA)
WO2011003901A1 (en) 2009-07-10 2011-01-13 Basf Plant Science Company Gmbh Expression cassettes for endosperm-specific expression in plants
US7872119B2 (en) 2007-02-26 2011-01-18 Quark Pharmaceuticals, Inc. Inhibitors of RTP801 and their use in disease treatment
WO2011008823A2 (en) 2009-07-14 2011-01-20 Mayo Foundation For Medical Education And Research Peptide-mediated non-covalent delivery of active agents across the blood brain barrier
WO2011011775A1 (en) 2009-07-24 2011-01-27 The Regents Of The University Of California Methods and compositions for treating and preventing disease associated with avb5 integrin
US7884086B2 (en) 2004-09-08 2011-02-08 Isis Pharmaceuticals, Inc. Conjugates for use in hepatocyte free uptake assays
US7893248B2 (en) 2002-02-20 2011-02-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Myc and/or Myb gene expression using short interfering nucleic acid (siNA)
US7897757B2 (en) 2002-02-20 2011-03-01 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of protein tyrosine phosphatase-1B (PTP-1B) gene expression using short interfering nucleic acid (siNA)
US7897753B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of XIAP gene expression using short interfering nucleic acid (siNA)
US7897752B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of telomerase gene expression using short interfering nucleic acid (siNA)
EP2292739A1 (fr) 2006-03-24 2011-03-09 Institut National De La Recherche Agronomique Procédé de préparation de cellules aviaires differenciées et gènes impliqués dans le maintien de la pluripotence
WO2011028218A1 (en) 2009-09-02 2011-03-10 Alnylam Pharmaceuticals, Inc. Process for triphosphate oligonucleotide synthesis
US7910724B2 (en) 2002-02-20 2011-03-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Fos gene expression using short interfering nucleic acid (siNA)
US7910566B2 (en) 2006-03-09 2011-03-22 Quark Pharmaceuticals Inc. Prevention and treatment of acute renal failure and other kidney diseases by inhibition of p53 by siRNA
US7910725B2 (en) 2002-02-20 2011-03-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
US7915479B2 (en) 2004-10-13 2011-03-29 University Of Georgia Research Foundation, Inc. Nematode resistant transgenic plants
US7915399B2 (en) 2006-06-09 2011-03-29 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US7919473B2 (en) 2004-03-12 2011-04-05 Alnylam Pharmaceuticals, Inc. IRNA agents targeting VEGF
US7923547B2 (en) 2002-09-05 2011-04-12 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7923549B2 (en) 2002-02-20 2011-04-12 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
US7928219B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of placental growth factor gene expression using short interfering nucleic acid (SINA)
US7928220B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of stromal cell-derived factor-1 (SDF-1) gene expression using short interfering nucleic acid (siNA)
US7928218B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of polycomb group protein EZH2 gene expression using short interfering nucleic acid (siNA)
US7935812B2 (en) 2002-02-20 2011-05-03 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of hepatitis C virus (HCV) expression using short interfering nucleic acid (siNA)
EP2316943A1 (en) 2007-07-05 2011-05-04 Novartis AG DSRNA for treating viral infection
US7943757B2 (en) 2002-02-20 2011-05-17 Mcswiggen James RNA interference mediated inhibition of intercellular adhesion molecule (ICAM) gene expression using short interfering nucleic acid (siNA)
US7947660B2 (en) 2005-03-11 2011-05-24 Alcon, Inc. RNAi-mediated inhibition of frizzled related protein-1 for treatment of glaucoma
WO2011063409A1 (en) 2009-11-23 2011-05-26 Aquabounty Technologies, Inc. Maternally induced sterility in animals
WO2011066475A1 (en) 2009-11-26 2011-06-03 Quark Pharmaceuticals, Inc. Sirna compounds comprising terminal substitutions
EP2330202A2 (en) 2006-01-06 2011-06-08 University Of Georgia Research Foundation, Inc. Cyst nematode resistant transgenic plants
WO2011067712A1 (en) 2009-12-03 2011-06-09 Basf Plant Science Company Gmbh Expression cassettes for embryo-specific expression in plants
WO2011072091A1 (en) 2009-12-09 2011-06-16 Quark Pharmaceuticals, Inc. Methods and compositions for treating diseases, disorders or injury of the cns
WO2011071916A2 (en) 2009-12-07 2011-06-16 The Johns Hopkins University Sr-bi as a predictor of human female infertility and responsiveness to treatment
WO2011071860A2 (en) 2009-12-07 2011-06-16 Alnylam Pharmaceuticals, Inc. Compositions for nucleic acid delivery
WO2011075656A1 (en) 2009-12-18 2011-06-23 The University Of British Columbia Methods and compositions for delivery of nucleic acids
US7968762B2 (en) 2004-07-13 2011-06-28 Van Andel Research Institute Immune-compromised transgenic mice expressing human hepatocyte growth factor (hHGF)
WO2011076807A2 (en) 2009-12-23 2011-06-30 Novartis Ag Lipids, lipid compositions, and methods of using them
US7977472B2 (en) 2002-02-20 2011-07-12 Leonid Beigelman RNA interference mediated inhibition of myostatin gene expression using short interfering nucleic acid (siNA)
WO2011084193A1 (en) 2010-01-07 2011-07-14 Quark Pharmaceuticals, Inc. Oligonucleotide compounds comprising non-nucleotide overhangs
US7985853B2 (en) 2002-02-20 2011-07-26 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of platelet derived growth factor (PDGF) and platelet derived growth factor receptor (PDGFR) gene expression using short interfering nucleic acid (siNA)
WO2011094345A1 (en) 2010-01-26 2011-08-04 National Jewish Health Methods and compositions for risk prediction, diagnosis, prognosis, and treatment of pulmonary disorders
WO2011094580A2 (en) 2010-01-28 2011-08-04 Alnylam Pharmaceuticals, Inc. Chelated copper for use in the preparation of conjugated oligonucleotides
WO2011100131A2 (en) 2010-01-28 2011-08-18 Alnylam Pharmacuticals, Inc. Monomers and oligonucleotides comprising cycloaddition adduct(s)
EP1423404B1 (en) * 2002-02-20 2011-08-24 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED TREATMENT OF ALZHEIMER'S DISEASE USING SHORT INTERFERING NUCLEIC ACID (siNA)
EP2360249A1 (en) 2005-03-31 2011-08-24 Calando Pharmaceuticals, Inc. Inhibitors of ribonucleotide reductase subunit 2 and uses thereof
EP2360253A1 (en) * 2000-03-30 2011-08-24 The Whitehead Institute for Biomedical Research RNA sequence-specific mediators of RNA interference
EP1572128B1 (en) * 2002-02-20 2011-08-24 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF HIV GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US8008472B2 (en) 2001-05-29 2011-08-30 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of human immunodeficiency virus (HIV) gene expression using short interfering nucleic acid (siNA)
US8008473B2 (en) 2002-02-20 2011-08-30 Mcswiggen James RNA interference mediated inhibition of TNF and TNF receptor gene expression using short interfering nucleic acid (siNA)
US8012474B2 (en) 2007-08-02 2011-09-06 Nov Immune S.A. Anti-RANTES antibodies
US8013143B2 (en) 2002-02-20 2011-09-06 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of CXCR4 gene expression using short interfering nucleic acid (siNA)
EP2363134A1 (en) 2005-11-09 2011-09-07 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor V Leiden mutant gene
US8017762B2 (en) 2003-04-17 2011-09-13 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
US8017761B2 (en) 2001-05-18 2011-09-13 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of Stearoyl-CoA desaturase (SCD) gene expression using short interfering nucelic acid (siNA)
US8022272B2 (en) 2001-07-13 2011-09-20 Sungene Gmbh & Co. Kgaa Expression cassettes for transgenic expression of nucleic acids
WO2011123621A2 (en) 2010-04-01 2011-10-06 Alnylam Pharmaceuticals Inc. 2' and 5' modified monomers and oligonucleotides
EP2377952A1 (en) 2007-04-26 2011-10-19 Ludwig Institute For Cancer Research Methods for diagnosing and treating astrocytomas
EP2380986A1 (en) 2006-01-12 2011-10-26 BASF Plant Science GmbH Use of stomatin (STM1) polynucleotides for achieving a pathogen resistance in plants
WO2011133868A2 (en) 2010-04-22 2011-10-27 Alnylam Pharmaceuticals, Inc. Conformationally restricted dinucleotide monomers and oligonucleotides
WO2011133876A2 (en) 2010-04-22 2011-10-27 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising acyclic and abasic nucleosides and analogs
WO2011133931A1 (en) 2010-04-22 2011-10-27 Genentech, Inc. Use of il-27 antagonists for treating inflammatory bowel disease
WO2011133871A2 (en) 2010-04-22 2011-10-27 Alnylam Pharmaceuticals, Inc. 5'-end derivatives
WO2011146938A1 (en) 2010-05-21 2011-11-24 NanoOncology, Inc. Reagents and methods for treating cancer
WO2011145777A1 (ko) 2010-05-20 2011-11-24 광주과학기술원 ＨＩＦ－２α 억제제를 유효성분으로 포함하는 관절염 예방 또는 치료용 약제학적 조성물
US8067575B2 (en) 2002-02-20 2011-11-29 Merck, Sharp & Dohme Corp. RNA interference mediated inhibition of cyclin D1 gene expression using short interfering nucleic acid (siNA)
US8067570B2 (en) 2006-01-20 2011-11-29 Quark Pharmaceuticals, Inc. Therapeutic uses of inhibitors of RTP801
EP2394662A2 (en) 2004-04-02 2011-12-14 The Regents of The University of California Methods and compositions for treating and preventing disease associated with AlphavBeta5 intergrin
WO2011163121A1 (en) 2010-06-21 2011-12-29 Alnylam Pharmaceuticals, Inc. Multifunctional copolymers for nucleic acid delivery
WO2011163466A1 (en) 2010-06-23 2011-12-29 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Regulation of skin pigmentation by neuregulin-1 (nrg-1)
US8088359B2 (en) 2004-06-04 2012-01-03 University Of Geneva Means and methods of using a NADPH oxidase inhibitor for the treatment of hearing loss and phantom hearing
US8093369B2 (en) 2005-10-11 2012-01-10 Ben Gurion University Of The Negev Research And Development Authority Ltd. Compositions for silencing the expression of VDAC1 and uses thereof
US8097422B2 (en) 2007-06-20 2012-01-17 Salk Institute For Biological Studies Kir channel modulators
EP2407483A1 (en) 2006-04-13 2012-01-18 Novartis Vaccines and Diagnostics, Inc. Methods of treating, diagnosing or detecting cancers
US8101741B2 (en) 2005-11-02 2012-01-24 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
EP2412383A1 (en) 2006-07-28 2012-02-01 Children's Memorial Hospital Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells
WO2012016188A2 (en) 2010-07-30 2012-02-02 Alnylam Pharmaceuticals, Inc. Methods and compositions for delivery of active agents
WO2012016184A2 (en) 2010-07-30 2012-02-02 Alnylam Pharmaceuticals, Inc. Methods and compositions for delivery of active agents
WO2012018754A2 (en) 2010-08-02 2012-02-09 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF CATENIN (CADHERIN-ASSOCIATED PROTEIN), BETA 1 (CTNNB1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2012024170A2 (en) 2010-08-17 2012-02-23 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF HEPATITIS B VIRUS (HBV) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2012027467A1 (en) 2010-08-26 2012-03-01 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF PROLYL HYDROXYLASE DOMAIN 2 (PHD2) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2012027206A1 (en) 2010-08-24 2012-03-01 Merck Sharp & Dohme Corp. SINGLE-STRANDED RNAi AGENTS CONTAINING AN INTERNAL, NON-NUCLEIC ACID SPACER
EP2431053A1 (en) 2006-11-27 2012-03-21 Patrys Limited Novel glycosylated peptide target in neoplastic cells
EP2434019A1 (en) 2003-08-01 2012-03-28 BASF Plant Science GmbH Process for the production of fine chemicals
US8153776B2 (en) 2000-03-16 2012-04-10 Cold Spring Harbor Laboratory Methods and compositions for RNA interference
EP2441837A1 (en) 2007-03-26 2012-04-18 Alnylam Pharmaceuticals Inc. dsRNA compositions and methods for treating HPV infections
WO2012051567A2 (en) 2010-10-15 2012-04-19 The Trustees Of Columbia University In The City Of New York Obesity-related genes and their proteins and uses thereof
WO2012058210A1 (en) 2010-10-29 2012-05-03 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACIDS (siNA)
JP2012085641A (ja) * 1999-11-19 2012-05-10 Cancer Research Technology Ltd Ｄｓｒｎａによる遺伝子発現の阻害
WO2012061443A2 (en) 2010-11-01 2012-05-10 NanoOncology, Inc. Compositions of a peptide-based system for cell-specific targeting
WO2012061537A2 (en) 2010-11-02 2012-05-10 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
WO2012064824A1 (en) 2010-11-09 2012-05-18 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of eg5 and vegf genes
US8183221B2 (en) 2007-09-05 2012-05-22 Medtronic, Inc. Suppression of SCN9A gene expression and/or function for the treatment of pain
WO2012078536A2 (en) 2010-12-06 2012-06-14 Quark Pharmaceuticals, Inc. Double stranded oligonucleotide compounds comprising positional modifications
WO2012078209A1 (en) 2010-12-06 2012-06-14 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Diagnosis and treatment of adrenocortical tumors using human microrna-483
US8202845B2 (en) 2002-04-18 2012-06-19 Acuity Pharmaceuticals, Inc. Means and methods for the specific modulation of target genes in the CNS and the eye and methods for their identification
EP2471809A1 (en) 2006-07-11 2012-07-04 University Of Medicine And Dentistry Of New Jersey Proteins, nucleic acids encoding the same and associated methods of use
WO2012090150A2 (en) 2010-12-27 2012-07-05 Compugen Ltd New cell-penetrating peptides and uses thereof
EP2474556A2 (en) 2007-03-14 2012-07-11 Novartis AG APCDD1 inhibitors for treating, diagnosing or detecting cancer
WO2012099755A1 (en) 2011-01-11 2012-07-26 Alnylam Pharmaceuticals, Inc. Pegylated lipids and their use for drug delivery
US8232256B2 (en) 2006-07-21 2012-07-31 Silence Therapeutics Ag Means for inhibiting the expression of protein kinase 3
EP2484780A1 (en) 2004-07-23 2012-08-08 The University of North Carolina At Chapel Hill Methods and materials for determining pain sensibility and predicting and treating related disorders
US8258288B2 (en) 2002-02-20 2012-09-04 Sirna Therapeutics, Inc. RNA interference mediated inhibition of respiratory syncytial virus (RSV) expression using short interfering nucleic acid (siNA)
WO2012118911A1 (en) 2011-03-03 2012-09-07 Quark Pharmaceuticals, Inc. Oligonucleotide modulators of the toll-like receptor pathway
WO2012118910A2 (en) 2011-03-03 2012-09-07 Quark Pharmaceuticals, Inc. Compositions and methods for treating lung disease and injury
US8273869B2 (en) 2009-06-15 2012-09-25 Alnylam Pharmaceuticals, Inc. Lipid formulated dsRNA targeting the PCSK9 gene
US8273866B2 (en) 2002-02-20 2012-09-25 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SINA)
US8278287B2 (en) 2008-04-15 2012-10-02 Quark Pharmaceuticals Inc. siRNA compounds for inhibiting NRF2
US8283332B2 (en) 2009-04-17 2012-10-09 University Of Louisville Research Foundation, Inc. PFKFB4 inhibitors and methods of using the same
US8299042B2 (en) 2002-04-26 2012-10-30 Alnylam Pharmaceuticals, Inc. Methods and compositions for silencing genes without inducing toxicity
EP2526968A2 (en) 2006-01-27 2012-11-28 Biogen Idec MA Inc. Nogo receptor antagonists
US8329667B2 (en) 2004-04-23 2012-12-11 The Trustees Of Columbia University In The City Of New York Inhibition of hairless protein mRNA
US8329890B2 (en) 2002-08-05 2012-12-11 University Of Iowa Research Foundation SiRNA-mediated gene silencing
US8329989B2 (en) 2008-09-29 2012-12-11 Monsanto Technology Llc Soybean transgenic event MON87705 and methods for detection thereof
WO2012170957A2 (en) 2011-06-08 2012-12-13 Nitto Denko Corporation Retinoid-liposomes for enhancing modulation of hsp47 expression
WO2012177947A2 (en) 2011-06-21 2012-12-27 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of expression of apolipoprotein c-iii (apoc3) genes
WO2012178033A2 (en) 2011-06-23 2012-12-27 Alnylam Pharmaceuticals, Inc. Serpina1 sirnas: compositions of matter and methods of treatment
WO2013003697A1 (en) 2011-06-30 2013-01-03 Trustees Of Boston University Method for controlling tumor growth, angiogenesis and metastasis using immunoglobulin containing and proline rich receptor-1 (igpr-1)
US8394947B2 (en) 2004-06-03 2013-03-12 Isis Pharmaceuticals, Inc. Positionally modified siRNA constructs
WO2013049328A1 (en) 2011-09-27 2013-04-04 Alnylam Pharmaceuticals, Inc. Di-aliphatic substituted pegylated lipids
US8426377B2 (en) 2003-04-17 2013-04-23 Alnylam Pharmaceuticals, Inc. iRNA agents with biocleavable tethers
WO2013059496A1 (en) 2011-10-18 2013-04-25 Dicerna Pharmaceuticals, Inc. Amine cationic lipids and uses thereof
EP2589660A1 (en) 2007-03-29 2013-05-08 Alnylam Pharmaceuticals Inc. Compositions and methods for inhibiting expression of a gene from the Ebola
WO2013067076A2 (en) 2011-11-03 2013-05-10 Quark Pharmaceuticals, Inc. Methods and compositions for neuroprotection
WO2013070821A1 (en) 2011-11-08 2013-05-16 Quark Pharmaceuticals, Inc. Methods and compositions for treating diseases, disorders or injury of the nervous system
US8470792B2 (en) 2008-12-04 2013-06-25 Opko Pharmaceuticals, Llc. Compositions and methods for selective inhibition of VEGF
EP2607477A1 (en) 2007-05-03 2013-06-26 The Brigham and Women's Hospital, Inc. Multipotent stem cells and uses thereof
US8476245B2 (en) 2004-02-11 2013-07-02 University Of Tennessee Research Foundation Inhibition of tumor growth and invasion by anti matrix metalloproteinase DNAzymes
US8481710B2 (en) 2002-08-05 2013-07-09 University Of Iowa Research Foundation RNA interference suppression of neurodegenerative diseases and methods of use thereof
US8491897B2 (en) 2007-08-23 2013-07-23 The Board Of Trustees Of The Leland Stanford Junior University Treatment of neuropathic pain using anti-thrombospondin antibodies
EP2617828A1 (en) 2007-12-10 2013-07-24 Alnylam Pharmaceuticals Inc. Compositions and methods for inhibiting expression of factor VII gene
US8507663B2 (en) 2010-04-06 2013-08-13 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of CD274/PD-L1 gene
WO2013119880A1 (en) 2012-02-07 2013-08-15 Global Bio Therapeutics Usa, Inc. Compartmentalized method of nucleic acid delivery and compositions and uses thereof
US8524879B2 (en) 2002-08-05 2013-09-03 University Of Iowa Research Foundation RNA interference suppresion of neurodegenerative diseases and methods of use thereof
US8541384B2 (en) 2002-07-24 2013-09-24 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiogenesis
WO2013149194A1 (en) 2012-03-29 2013-10-03 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
EP2657350A1 (en) 2007-07-14 2013-10-30 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2013165816A2 (en) 2012-05-02 2013-11-07 Merck Sharp & Dohme Corp. SHORT INTERFERING NUCLEIC ACID (siNA) COMPOSITIONS
US8586726B2 (en) 2007-07-18 2013-11-19 The Trustees Of Columbia University In The City Of New York Tissue-specific MicroRNAs and compositions and uses thereof
WO2013170960A1 (en) 2012-05-16 2013-11-21 Silence Therapeutics Ag Use of vegfr1 as a biomarker for pkn3 inhibitor administration
US8614311B2 (en) 2007-12-12 2013-12-24 Quark Pharmaceuticals, Inc. RTP801L siRNA compounds and methods of use thereof
WO2014006227A1 (en) 2012-07-06 2014-01-09 Institut Gustave-Roussy Simultaneous detection of cannibalism and senescence as prognostic marker for cancer
EP2687609A2 (en) 2008-11-10 2014-01-22 The United States of America As Represented by the Secretary Department of Health and Human Services Method for treating solid tumor
EP2690175A2 (en) 2008-09-02 2014-01-29 Alnylam Pharmaceuticals Compositions and methods for combined inhibition of mutant EGFR gene and IL-6 expression
WO2014018554A1 (en) 2012-07-23 2014-01-30 La Jolla Institute For Allergy And Immunology Ptprs and proteoglycans in autoimmune disease
EP2712926A2 (en) 2008-03-05 2014-04-02 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Eg5 and VEGF genes
US8697875B2 (en) 2008-12-23 2014-04-15 The Trustees Of Columbia University In The City Of New York Phosphodiesterase inhibitors and uses thereof
WO2014058915A2 (en) 2012-10-08 2014-04-17 St. Jude Children's Research Hospital Therapies based on control of regulatory t cell stability and function via a neuropilin-1:semaphorin axis
US8703769B2 (en) 2005-07-15 2014-04-22 The University Of North Carolina At Chapel Hill Use of EGFR inhibitors to prevent or treat obesity
US8710209B2 (en) 2009-12-09 2014-04-29 Nitto Denko Corporation Modulation of HSP47 expression
EP2727996A1 (en) 2008-11-06 2014-05-07 The Johns-Hopkins University Treatment of chronic inflammatory respiratory disorders with NP1 inhibitors
WO2014068072A1 (en) 2012-10-31 2014-05-08 Institut Gustave-Roussy Identification, assessment and therapy of essential thrombocythemia with resistance to jak2 inhibitors
US8729036B2 (en) 2002-08-07 2014-05-20 University Of Massachusetts Compositions for RNA interference and methods of use thereof
EP1572902B1 (en) * 2002-02-01 2014-06-11 Life Technologies Corporation HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
US8785408B2 (en) 2007-06-27 2014-07-22 Quark Pharmaceuticals, Inc. Compositions and methods for reducing or protecting against delayed graft function (DGF)
US8796436B2 (en) 2003-04-17 2014-08-05 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
DE10066235C5 (de) 1999-01-30 2014-08-21 Alnylam Europe Ag Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
WO2014136086A1 (en) 2013-03-08 2014-09-12 Novartis Ag Lipids and lipid compositions for the delivery of active agents
WO2014145254A2 (en) 2013-03-15 2014-09-18 Sutter West Bay Hospitals Falz for use as a target for therapies to treat cancer
US8841432B2 (en) 2011-04-21 2014-09-23 Snu R&Db Foundation Shuttle vectors for mycobacteria-escherichia coli and uses thereof
WO2014151734A1 (en) 2013-03-15 2014-09-25 The Trustees Of Columbia University In The City Of New York Fusion proteins and methods thereof
US8846868B2 (en) 2008-04-17 2014-09-30 Fusion Antibodies Limited Anti-AREG/HB-EGF antibodies and treatment
US8846894B2 (en) 2002-02-20 2014-09-30 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
WO2014160871A2 (en) 2013-03-27 2014-10-02 The General Hospital Corporation Methods and agents for treating alzheimer's disease
US8906381B2 (en) 2008-10-12 2014-12-09 Massachusetts Institute Of Technology Immunonanotherapeutics that provide IGG humoral response without T-cell antigen
EP2810643A2 (en) 2009-08-14 2014-12-10 Alnylam Pharmaceuticals Inc. Lipid formulated compositions and mehods for inhibiting expression of a gene from the ebola virus
US8932595B2 (en) 2008-10-12 2015-01-13 Massachusetts Institute Of Technology Nicotine immunonanotherapeutics
US8946177B2 (en) 2004-11-12 2015-02-03 Mima Therapeutics, Inc Methods and compositions involving miRNA and miRNA inhibitor molecules
WO2015015498A1 (en) 2013-07-31 2015-02-05 Qbi Enterprises Ltd. Methods of use of sphingolipid polyalkylamine oligonucleotide compounds
WO2015015496A1 (en) 2013-07-31 2015-02-05 Qbi Enterprises Ltd. Sphingolipid-polyalkylamine-oligonucleotide compounds
US8968702B2 (en) 2006-03-30 2015-03-03 Duke University Inhibition of HIF-1 activation for anti-tumor and anti-inflammatory responses
WO2015051045A2 (en) 2013-10-04 2015-04-09 Novartis Ag 3'END CAPS FOR RNAi AGENTS FOR USE IN RNA INTERFERENCE
WO2015051044A2 (en) 2013-10-04 2015-04-09 Novartis Ag Novel formats for organic compounds for use in rna interference
WO2015050871A2 (en) 2013-10-04 2015-04-09 Novartis Ag Organic compounds to treat hepatitis b virus
WO2015051366A2 (en) 2013-10-04 2015-04-09 Novartis Ag Novel formats for organic compounds for use in rna interference
WO2015054451A1 (en) 2013-10-09 2015-04-16 The United States Of America As Represented By The Secretary Department Of Health And Human Services Detection of hepatitis delta virus (hdv) for the diagnosis and treatment of sjögren's syndrome and lymphoma
US20150105445A1 (en) * 2001-05-18 2015-04-16 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING CHEMICALLY MODIFIED SHORT INTERFERING NUCLEIC ACID (siNA)
WO2015054670A1 (en) 2013-10-11 2015-04-16 Genentech, Inc. Nsp4 inhibitors and methods of use
US9029527B2 (en) 1998-03-20 2015-05-12 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs
US9051567B2 (en) 2009-06-15 2015-06-09 Tekmira Pharmaceuticals Corporation Methods for increasing efficacy of lipid formulated siRNA
WO2015095346A1 (en) 2013-12-19 2015-06-25 Novartis Ag Lipids and lipid compositions for the delivery of active agents
WO2015095340A1 (en) 2013-12-19 2015-06-25 Novartis Ag Lipids and lipid compositions for the delivery of active agents
US9066978B2 (en) 2010-05-26 2015-06-30 Selecta Biosciences, Inc. Dose selection of adjuvanted synthetic nanocarriers
WO2015098113A1 (ja) 2013-12-27 2015-07-02 独立行政法人医薬基盤研究所 悪性腫瘍の治療薬
US9074213B2 (en) 2001-01-09 2015-07-07 Alnylam Pharmacuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US9080215B2 (en) 2007-09-14 2015-07-14 Asuragen, Inc. MicroRNAs differentially expressed in cervical cancer and uses thereof
US9080014B2 (en) 2006-05-15 2015-07-14 Massachusetts Institute Of Technology Polymers for functional particles
US9096636B2 (en) 1996-06-06 2015-08-04 Isis Pharmaceuticals, Inc. Chimeric oligomeric compounds and their use in gene modulation
EP2913341A1 (en) 2006-12-22 2015-09-02 University of Utah Research Foundation Method of detecting ocular diseases and pathologic conditions and treatment of same
US9150606B2 (en) 2002-11-05 2015-10-06 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2'-modified nucleosides for use in gene modulation
US9150605B2 (en) 2002-11-05 2015-10-06 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2′-modified nucleosides for use in gene modulation
EP2937418A1 (en) 2008-10-20 2015-10-28 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of transthyretin
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
KR20150129189A (ko) 2014-05-08 2015-11-19 서울대학교산학협력단 도파민 의존성 신경장애 치료를 위한 REV-ERBα 유전자의 용도
KR20150129190A (ko) 2014-05-08 2015-11-19 서울대학교산학협력단 정서장애 및 중독질환 치료를 위한 REV-ERBα 유전자의 용도
US9198911B2 (en) 2010-11-02 2015-12-01 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
US9217129B2 (en) 2007-02-09 2015-12-22 Massachusetts Institute Of Technology Oscillating cell culture bioreactor
US9228188B2 (en) 2011-06-21 2016-01-05 Alnylam Pharmaceuticals, Inc. Compositions and method for inhibiting hepcidin antimicrobial peptide (HAMP) or HAMP-related gene expression
US9228186B2 (en) 2002-11-14 2016-01-05 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
WO2016010840A1 (en) 2014-07-16 2016-01-21 Novartis Ag Method of encapsulating a nucleic acid in a lipid nanoparticle host
EP2980220A1 (en) 2005-09-20 2016-02-03 BASF Plant Science GmbH Improved methods controlling gene expression
US9267937B2 (en) 2005-12-15 2016-02-23 Massachusetts Institute Of Technology System for screening particles
US9274117B2 (en) 2013-12-21 2016-03-01 Catholic University Industry Academic Use of SIRT7 as novel cancer therapy target and method for treating cancer using the same
WO2016037053A1 (en) 2014-09-05 2016-03-10 Novartis Ag Lipids and lipid compositions for the delivery of active agents
WO2016044271A2 (en) 2014-09-15 2016-03-24 Children's Medical Center Corporation Methods and compositions to increase somatic cell nuclear transfer (scnt) efficiency by removing histone h3-lysine trimethylation
US9308280B2 (en) 2008-10-12 2016-04-12 Massachusetts Institute Of Technology Targeting of antigen presenting cells with immunonanotherapeutics
WO2016057693A1 (en) 2014-10-10 2016-04-14 Alnylam Pharmaceuticals, Inc. Methods and compositions for inhalation delivery of conjugated oligonucleotide
US9333179B2 (en) 2007-04-04 2016-05-10 Massachusetts Institute Of Technology Amphiphilic compound assisted nanoparticles for targeted delivery
WO2016077624A1 (en) 2014-11-12 2016-05-19 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
US9365852B2 (en) 2008-05-08 2016-06-14 Mirna Therapeutics, Inc. Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
WO2016106400A2 (en) 2014-12-26 2016-06-30 Nitto Denko Corporation Rna interference agents for gst-pi gene modulation
US9381477B2 (en) 2006-06-23 2016-07-05 Massachusetts Institute Of Technology Microfluidic synthesis of organic nanoparticles
EP3040081A2 (en) 2002-08-14 2016-07-06 Silence Therapeutics GmbH Use of protein kinase n beta
US9387262B2 (en) 2004-12-27 2016-07-12 Silence Therapeutics Gmbh Coated lipid complexes and their use
WO2016140921A1 (en) 2015-03-02 2016-09-09 180 Therapeutics Lp Method of treating a localized fibrotic disorder using an il-33 antagonist
US9439859B2 (en) 2008-10-12 2016-09-13 Massachusetts Institute Of Technology Adjuvant incorporation in immunoanotherapeutics
US9446062B2 (en) 2006-10-25 2016-09-20 Quark Pharmaceuticals, Inc. Methods of treating ischemia-reperfusion injury with siRNAs
EP3072555A1 (en) 2011-09-02 2016-09-28 The Trustees of Columbia University in the City of New York Mk2/3 inhibitors to treat metabolic disturbances of obesity
US9474717B2 (en) 2007-10-12 2016-10-25 Massachusetts Institute Of Technology Vaccine nanotechnology
WO2016176617A2 (en) 2015-04-29 2016-11-03 New York University Method for treating high-grade gliomas
US9492400B2 (en) 2004-11-04 2016-11-15 Massachusetts Institute Of Technology Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals
WO2016201389A2 (en) 2015-06-12 2016-12-15 Alector Llc Anti-cd33 antibodies and methods of use thereof
WO2016201388A2 (en) 2015-06-12 2016-12-15 Alector Llc Anti-cd33 antibodies and methods of use thereof
EP3109321A1 (en) 2008-09-25 2016-12-28 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of serum amyloid a gene
WO2016210292A1 (en) 2015-06-25 2016-12-29 Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion, enrichment, and maintenance
WO2017040301A1 (en) 2015-08-28 2017-03-09 Alector Llc Anti-siglec-7 antibodies and methods of use thereof
US9592250B2 (en) 2002-02-01 2017-03-14 Life Technologies Corporation Double-stranded oligonucleotides
EP3141265A1 (en) 2007-12-04 2017-03-15 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
WO2017075432A2 (en) 2015-10-29 2017-05-04 Alector Llc Anti-siglec-9 antibodies and methods of use thereof
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9669103B2 (en) 2015-03-18 2017-06-06 Korea National University Of Transportation Industry-Academic Cooperation Foundation Metal-particle-based oral-administrating liver-specific nucleic acid delivery system and method of manufacturing the same
US9682123B2 (en) 2013-12-20 2017-06-20 The Trustees Of Columbia University In The City Of New York Methods of treating metabolic disease
US9708621B2 (en) 1999-08-13 2017-07-18 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US9719092B2 (en) 2002-11-14 2017-08-01 Thermo Fisher Scientific Inc. RNAi targeting CNTD2
US9719094B2 (en) 2002-11-14 2017-08-01 Thermo Fisher Scientific Inc. RNAi targeting SEC61G
US9738727B2 (en) 2011-10-14 2017-08-22 Genentech, Inc. Anti-HtrA1 antibodies and methods of use
US9751933B2 (en) 2012-06-14 2017-09-05 Scripps Korea Antibody Institute Antibody specific for CLEC14A and uses thereof
WO2017152073A1 (en) 2016-03-04 2017-09-08 University Of Louisville Research Foundation, Inc. Methods and compositions for ex vivo expansion of very small embryonic-like stem cells (vsels)
US9765351B2 (en) 2006-02-13 2017-09-19 Monsanto Technology Llc Modified gene silencing
WO2017161001A1 (en) 2016-03-15 2017-09-21 Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion
US9771586B2 (en) 2002-11-14 2017-09-26 Thermo Fisher Scientific Inc. RNAi targeting ZNF205
EP3222294A1 (en) 2003-04-30 2017-09-27 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US9796979B2 (en) 2011-03-03 2017-10-24 Quark Pharmaceuticals Inc. Oligonucleotide modulators of the toll-like receptor pathway
US9839649B2 (en) 2002-11-14 2017-12-12 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US9879266B2 (en) 2002-11-14 2018-01-30 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
WO2018020012A1 (en) 2016-07-29 2018-02-01 Danmarks Tekniske Universitet Methods for decoupling cell growth from production of biochemicals and recombinant polypeptides
US9885036B2 (en) 2008-07-01 2018-02-06 Daiichi Sankyo Company, Limited Double-stranded polynucleotide
WO2018057575A1 (en) 2016-09-21 2018-03-29 Alnylam Pharmaceuticals, Inc Myostatin irna compositions and methods of use thereof
US9963698B2 (en) 1998-03-20 2018-05-08 Commonwealth Scientific And Industrial Research Organisation Control of gene expression
US9969677B2 (en) 2010-12-22 2018-05-15 The Trustees Of Columbia University In The City Of New York Histone acetyltransferase modulators and uses thereof
EP3329924A1 (en) 2010-03-29 2018-06-06 Alnylam Pharmaceuticals, Inc. Sirna therapy for transthyretin (ttr) related ocular amyloidosis
US9994915B2 (en) 2010-10-11 2018-06-12 Sanford-Burnham Medical Research Institute miR-211 expression and related pathways in human melanoma
US9994853B2 (en) 2001-05-18 2018-06-12 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
US10004814B2 (en) 2013-11-11 2018-06-26 Sirna Therapeutics, Inc. Systemic delivery of myostatin short interfering nucleic acids (siNA) conjugated to a lipophilic moiety
US10011836B2 (en) 2002-11-14 2018-07-03 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US10047388B2 (en) 2004-05-28 2018-08-14 Asuragen, Inc. Methods and compositions involving MicroRNA
US10072065B2 (en) 2015-08-24 2018-09-11 Mayo Foundation For Medical Education And Research Peptide-mediated delivery of immunoglobulins across the blood-brain barrier
EP3388068A1 (en) 2011-06-21 2018-10-17 Alnylam Pharmaceuticals, Inc. Composition and methods for inhibition of expression of protein c (proc) genes
WO2018213316A1 (en) 2017-05-16 2018-11-22 Alector Llc Anti-siglec-5 antibodies and methods of use thereof
US10196637B2 (en) 2011-06-08 2019-02-05 Nitto Denko Corporation Retinoid-lipid drug carrier
WO2019047914A1 (zh) 2017-09-07 2019-03-14 北京泰德制药股份有限公司 靶向ckip-1的双链rna分子及其用途
EP3456827A2 (en) 2010-06-02 2019-03-20 Alnylam Pharmaceuticals, Inc. Compositions and methods directed to treating liver fibrosis
WO2019126651A1 (en) 2017-12-21 2019-06-27 Alnylam Pharmaceuticals, Inc. Chirally-enriched double-stranded rna agents
US10421822B2 (en) 2015-10-30 2019-09-24 Genetech, Inc. Anti-HtrA1 antibodies and methods of use thereof
WO2019199673A1 (en) 2018-04-09 2019-10-17 President And Fellows Of Harvard College Modulating nuclear receptors and methods of using same
EP3564393A1 (en) 2011-06-21 2019-11-06 Alnylam Pharmaceuticals, Inc. Assays and methods for determining activity of a therapeutic agent in a subject
US10508277B2 (en) 2004-05-24 2019-12-17 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
EP3587434A1 (en) 2008-09-23 2020-01-01 Alnylam Pharmaceuticals Inc. Chemical modifications of monomers and oligonucleotides with click components for conjugation with ligands
WO2020023920A1 (en) 2018-07-27 2020-01-30 Alector Llc Anti-siglec-5 antibodies and methods of use thereof
EP3604533A1 (en) 2008-04-11 2020-02-05 Arbutus Biopharma Corporation Site-specific delivery of nucleic acids by combining targeting ligands with endosomolytic components
WO2020061381A1 (en) 2018-09-19 2020-03-26 La Jolla Institute For Immunology Ptprs and proteoglycans in rheumatoid arthritis
EP3632901A1 (en) 2009-12-10 2020-04-08 The Trustees of Columbia University in the City of New York Histone acetyltransferase activators and uses thereof
US10640457B2 (en) 2009-12-10 2020-05-05 The Trustees Of Columbia University In The City Of New York Histone acetyltransferase activators and uses thereof
US10711062B2 (en) 2017-08-03 2020-07-14 Alector Llc Anti-CD33 antibodies and methods of use thereof
EP3693384A1 (en) 2014-03-11 2020-08-12 Cellectis Method for generating t-cells compatible for allogenic transplantation
EP3699592A1 (en) 2013-03-13 2020-08-26 Geneweave Biosciences Inc. Non-replicative transduction particles and transduction particle-based reporter systems
EP3702470A2 (en) 2015-09-09 2020-09-02 The Trustees of Columbia University in the City of New York Reduction of er-mam-localized app-c99 and methods of treating alzheimer's disease
US10781446B2 (en) 2015-03-09 2020-09-22 University Of Kentucky Research Foundation RNA nanoparticle for treatment of gastric cancer
US10857145B2 (en) 2015-09-29 2020-12-08 Duke University Compositions and methods for identifying and treating dystonia disorders
US10883108B2 (en) 2016-03-31 2021-01-05 The Schepens Eye Research Institute, Inc. Endomucin inhibitor as an anti-angiogenic agent
US10889813B2 (en) 2015-09-02 2021-01-12 Alnylam Pharmaceuticals, Inc. Programmed cell death 1 ligand 1 (PD-L1) iRNA compositions and methods of use thereof
EP3778644A2 (en) 2014-12-23 2021-02-17 The Trustees of Columbia University in the City of New York Fgfr-tacc fusion proteins and methods thereof
WO2021037972A1 (en) 2019-08-27 2021-03-04 Sanofi Compositions and methods for inhibiting pcsk9
WO2021060624A1 (ko) 2019-09-24 2021-04-01 테고사이언스(주) 켈로이드 또는 비후성 반흔 예방 또는 치료용 조성물
EP3816287A1 (en) 2015-12-13 2021-05-05 Nitto Denko Corporation Sirna structures for high activity and reduced off target
WO2021123920A1 (en) 2019-12-18 2021-06-24 Novartis Ag Compositions and methods for the treatment of hemoglobinopathies
WO2021124172A1 (en) 2019-12-18 2021-06-24 Novartis Ag 3-(5-methoxy-1-oxoisoindolin-2-yl)piperidine-2,6-dione derivatives and uses thereof
WO2021142191A1 (en) 2020-01-08 2021-07-15 Regeneron Pharmaceuticals, Inc. Treatment of fibrodysplasia ossificans progressiva
WO2021150300A1 (en) 2020-01-22 2021-07-29 Massachusetts Institute Of Technology Inducible tissue constructs and uses thereof
US11085044B2 (en) 2015-03-09 2021-08-10 University Of Kentucky Research Foundation miRNA for treatment of breast cancer
WO2021173965A1 (en) 2020-02-28 2021-09-02 Massachusetts Institute Of Technology Identification of variable influenza residues and uses thereof
US11141492B2 (en) 2013-07-03 2021-10-12 City Of Hope Anticancer combinations
US11147249B2 (en) 2016-12-08 2021-10-19 Alector Llc Siglec transgenic mice and methods of use thereof
US11279768B1 (en) 2015-04-03 2022-03-22 Precision Biologics, Inc. Anti-cancer antibodies, combination therapies, and uses thereof
US11325939B2 (en) 2015-03-09 2022-05-10 University Of Kentucky Research Foundation RNA nanoparticles for brain tumor treatment
US11364032B2 (en) 2013-08-08 2022-06-21 Global Bio Therapeutics, Inc. Clamp device for minimally invasive procedures and uses thereof
WO2022147480A1 (en) 2020-12-30 2022-07-07 Ansun Biopharma, Inc. Oncolytic virus encoding sialidase and multispecific immune cell engager
EP4035659A1 (en) 2016-11-29 2022-08-03 PureTech LYT, Inc. Exosomes for delivery of therapeutic agents
US11466061B2 (en) 2014-08-22 2022-10-11 Yingfang Liu Methods and compositions for treating and/or preventing a disease or disorder associated with abnormal level and/or activity of the IFP35 family of proteins
US11470827B2 (en) 2017-12-12 2022-10-18 Alector Llc Transgenic mice expressing human TREM proteins and methods of use thereof
WO2022247917A1 (zh) 2021-05-28 2022-12-01 上海瑞宏迪医药有限公司 衣壳变异的重组腺相关病毒及其应用
WO2022269518A2 (en) 2021-06-23 2022-12-29 Novartis Ag Compositions and methods for the treatment of hemoglobinopathies
WO2023012165A1 (en) 2021-08-02 2023-02-09 Universite De Montpellier Compositions and methods for treating cmt1a or cmt1e diseases with rnai molecules targeting pmp22
EP4206216A1 (en) 2016-05-13 2023-07-05 4D Molecular Therapeutics Inc. Adeno-associated virus variant capsids and methods of use thereof
US11708575B2 (en) 2018-11-16 2023-07-25 Nitto Denko Corporation RNA interference delivery formulation and methods for malignant tumors
EP4218828A2 (en) 2017-09-20 2023-08-02 4D Molecular Therapeutics Inc. Adeno-associated virus variant capsids and methods of use thereof
EP4219695A2 (en) 2017-11-27 2023-08-02 4D Molecular Therapeutics Inc. Adeno-associated virus variant capsids and use for inhibiting angiogenesis
WO2024059824A2 (en) 2022-09-16 2024-03-21 Arsenal Biosciences, Inc. Immune cells with combination gene perturbations
WO2024059618A2 (en) 2022-09-13 2024-03-21 Arsenal Biosciences, Inc. Immune cells having co-expressed tgfbr shrnas
US11964028B2 (en) 2015-04-17 2024-04-23 University Of Kentucky Research Foundation RNA nanoparticles and method of use thereof
US12037407B2 (en) 2021-10-14 2024-07-16 Arsenal Biosciences, Inc. Immune cells having co-expressed shRNAS and logic gate systems
WO2024186656A1 (en) 2023-03-03 2024-09-12 Arsenal Biosciences, Inc. Systems targeting psma and ca9
US12178902B1 (en) 2020-01-12 2024-12-31 University Of Southern California Methods and compositions for fluid drainage by Piezo ion channel activation
US12215382B2 (en) 2019-03-01 2025-02-04 The General Hospital Corporation Liver protective MARC variants and uses thereof
US12227568B2 (en) 2018-06-08 2025-02-18 Alector Llc Anti-Siglec-7 antibodies and methods of use thereof
US12247073B2 (en) 2018-08-31 2025-03-11 Alector Llc Anti-CD33 antibodies and methods of use thereof
WO2025199338A1 (en) 2024-03-20 2025-09-25 Arsenal Biosciences, Inc. Systems targeting slc34a2 and tmprss4 and methods of use thereof
US12458604B2 (en) 2020-10-14 2025-11-04 The Trustees Of The University Of Pennsylvania Methods of lipid nanoparticle manufacture and compositions derived therefrom
US12460206B2 (en) 2012-12-05 2025-11-04 Alnylam Pharmaceuticals, Inc. PCSK9 iRNA compositions and methods of use thereof

Families Citing this family (180)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030206887A1 (en) * 1992-05-14 2003-11-06 David Morrissey RNA interference mediated inhibition of hepatitis B virus (HBV) using short interfering nucleic acid (siNA)
CA2361201A1 (en) * 1999-01-28 2000-08-03 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna
US20070021979A1 (en) * 1999-04-16 2007-01-25 Cosentino Daniel L Multiuser wellness parameter monitoring system
BR0009884A (pt) * 1999-04-21 2002-01-08 American Home Prod Processos e composições para a inibição da função das sequências de polinucleotìdeos
GB9925459D0 (en) 1999-10-27 1999-12-29 Plant Bioscience Ltd Gene silencing
WO2003070918A2 (en) 2002-02-20 2003-08-28 Ribozyme Pharmaceuticals, Incorporated Rna interference by modified short interfering nucleic acid
US20020173478A1 (en) * 2000-11-14 2002-11-21 The Trustees Of The University Of Pennsylvania Post-transcriptional gene silencing by RNAi in mammalian cells
DE10100588A1 (de) * 2001-01-09 2002-07-18 Ribopharma Ag Verfahren zur Hemmung der Expression eines Zielgens
EP1386004A4 (en) 2001-04-05 2005-02-16 Ribozyme Pharm Inc MODULATION OF GENE EXPRESSION ASSOCIATED WITH INFLAMMATORY PROLIFERATION AND GROWTH OF NEURITIES BY METHODS INVOLVING NUCLEIC ACID
US20050158735A1 (en) * 2001-05-18 2005-07-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of proliferating cell nuclear antigen (PCNA) gene expression using short interfering nucleic acid (siNA)
WO2003102131A2 (en) * 2002-04-22 2003-12-11 Sirna Therapeutics Inc. Nucleic acid mediated disruption of hiv fusogenic peptide interactions
US20050143333A1 (en) * 2001-05-18 2005-06-30 Sirna Therapeutics, Inc. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (SINA)
US20050159381A1 (en) * 2001-05-18 2005-07-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of chromosome translocation gene expression using short interfering nucleic acid (siNA)
US20050196767A1 (en) * 2001-05-18 2005-09-08 Sirna Therapeutics, Inc. RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acis (siNA)
WO2003070884A2 (en) * 2002-02-20 2003-08-28 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF MDR P-GLYCOPROTEIN GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20050159382A1 (en) * 2001-05-18 2005-07-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of polycomb group protein EZH2 gene expression using short interfering nucleic acid (siNA)
US20050288242A1 (en) * 2001-05-18 2005-12-29 Sirna Therapeutics, Inc. RNA interference mediated inhibition of RAS gene expression using short interfering nucleic acid (siNA)
US20050203040A1 (en) * 2001-05-18 2005-09-15 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular cell adhesion molecule (VCAM) gene expression using short interfering nucleic acid (siNA)
US20050196765A1 (en) * 2001-05-18 2005-09-08 Sirna Therapeutics, Inc. RNA interference mediated inhibition of checkpoint Kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA)
US20040198682A1 (en) * 2001-11-30 2004-10-07 Mcswiggen James RNA interference mediated inhibition of placental growth factor gene expression using short interfering nucleic acid (siNA)
US20050233997A1 (en) * 2001-05-18 2005-10-20 Sirna Therapeutics, Inc. RNA interference mediated inhibition of matrix metalloproteinase 13 (MMP13) gene expression using short interfering nucleic acid (siNA)
US20050124566A1 (en) * 2001-05-18 2005-06-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of myostatin gene expression using short interfering nucleic acid (siNA)
US20050176025A1 (en) * 2001-05-18 2005-08-11 Sirna Therapeutics, Inc. RNA interference mediated inhibition of B-cell CLL/Lymphoma-2 (BCL-2) gene expression using short interfering nucleic acid (siNA)
WO2003070972A2 (en) * 2002-02-20 2003-08-28 Sirna Therapeutics Inc. RNA INTERFERENCE MEDIATED INHIBITION OF CHROMOSOME TRANSLOCATION GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20050182007A1 (en) * 2001-05-18 2005-08-18 Sirna Therapeutics, Inc. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (SINA)
DK1409506T3 (da) 2001-07-23 2012-08-06 Univ Leland Stanford Junior Fremgangsmåder og sammensætninger til RNAi-formidlet inhibering af genekspression i pattedyr
US10590418B2 (en) 2001-07-23 2020-03-17 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for RNAi mediated inhibition of gene expression in mammals
EP1465910A4 (en) * 2002-02-20 2005-03-16 Sirna Therapeutics Inc INHIBITION OF GENE EXPRESSION OF CHECKPOINT KINASE-1 (CHK-1) IMPROVED BY RNA INTERFERENCE USING SHORT INTERFERING NUCLEIC ACIDS
US20090247613A1 (en) * 2002-02-20 2009-10-01 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF B-CELL CLL/LYMPHOMA-2 (BCL2) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
EP1472265A4 (en) * 2002-02-20 2005-06-15 Sirna Therapeutics Inc RNA INTERFERENCE MEDIA INHIBITION OF THE EXPRESSION OF THE POLYCOMB GROUP EZH2 PROTEIN GENE USING A SHORT INTERFERING NUCLEIC ACID (SINA)
WO2003070966A2 (en) * 2002-02-20 2003-08-28 Sirna Therapeutics, Inc RNA INTERFERENCE MEDIATED TARGET DISCOVERY AND TARGET VALIDATION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20090137510A1 (en) * 2002-02-20 2009-05-28 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF NF-KAPPA B/ REL-A GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US20100075423A1 (en) * 2002-06-12 2010-03-25 Life Technologies Corporation Methods and compositions relating to polypeptides with rnase iii domains that mediate rna interference
US20040248094A1 (en) * 2002-06-12 2004-12-09 Ford Lance P. Methods and compositions relating to labeled RNA molecules that reduce gene expression
EP1532271A4 (en) * 2002-06-12 2006-10-18 Ambion Inc METHODS AND COMPOSITIONS RELATED TO POLYPEPTIDES WITH RNASE III DOMAINS THAT TRANSFER RNA INTERFERENCE
US20040023390A1 (en) * 2002-08-05 2004-02-05 Davidson Beverly L. SiRNA-mediated gene silencing with viral vectors
US20050255086A1 (en) * 2002-08-05 2005-11-17 Davidson Beverly L Nucleic acid silencing of Huntington's Disease gene
US20040029275A1 (en) * 2002-08-10 2004-02-12 David Brown Methods and compositions for reducing target gene expression using cocktails of siRNAs or constructs expressing siRNAs
JP4339852B2 (ja) 2002-08-12 2009-10-07 ニュー・イングランド・バイオラブズ・インコーポレイティッド 遺伝子サイレンシングに関する方法および組成物
US20040053289A1 (en) * 2002-09-09 2004-03-18 The Regents Of The University Of California Short interfering nucleic acid hybrids and methods thereof
US20040242518A1 (en) * 2002-09-28 2004-12-02 Massachusetts Institute Of Technology Influenza therapeutic
CA2504926C (en) 2002-11-01 2014-01-14 The Trustees Of The University Of Pennsylvania Compositions and methods for sirna inhibition of hif-1 alpha
CA2513623A1 (en) 2003-01-16 2004-08-05 The Trustees Of The University Of Pennsylvania Compositions and methods for sirna inhibition of icam-1
US20090298914A1 (en) * 2003-04-15 2009-12-03 Sirna Therapeutics, Inc. RNA Interference Mediated Inhibition of Severe Acute Respiratory Syndrome (SARS) Virus Gene Expression Using Short Interfering Nucleic Acid (siNA)
US7994305B2 (en) 2003-04-18 2011-08-09 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiopoietin 1 and 2 and their receptor Tie2
AU2004253455B2 (en) * 2003-06-03 2011-03-03 Eli Lilly And Company Modulation of survivin expression
AU2004252559B2 (en) * 2003-06-25 2010-07-29 Gencia Corporation Modified vectors for organelle transfection
JP2007503803A (ja) 2003-08-28 2007-03-01 ノバルティス アクチエンゲゼルシャフト 平滑末端および３’修飾を有する干渉性ｒｎａ二本鎖
US20070202505A1 (en) * 2003-09-08 2007-08-30 Alex Chenchik Methods for gene function analysis
WO2005047506A1 (en) * 2003-11-04 2005-05-26 Geron Corporation Rna amidates and thioamidates for rnai
EP3427585B1 (en) * 2003-11-26 2024-10-16 University of Massachusetts Sequence-specific inhibition of small rna function
WO2005073378A1 (en) * 2004-01-30 2005-08-11 Santaris Pharma A/S MODIFIED SHORT INTERFERING RNA (MODIFIED siRNA)
EP2330111A3 (en) 2004-01-30 2011-08-17 Quark Pharmaceuticals, Inc. Oligoribonucleotides and methods of use thereof for treatment of fibrotic conditions and other diseases
US20090280567A1 (en) * 2004-02-06 2009-11-12 Dharmacon, Inc. Stabilized sirnas as transfection controls and silencing reagents
US20070269889A1 (en) * 2004-02-06 2007-11-22 Dharmacon, Inc. Stabilized siRNAs as transfection controls and silencing reagents
KR101147147B1 (ko) * 2004-04-01 2012-05-25 머크 샤프 앤드 돔 코포레이션 Ｒｎａ 간섭의 오프 타겟 효과 감소를 위한 변형된폴리뉴클레오타이드
DE102004025881A1 (de) 2004-05-19 2006-01-05 Beiersdorf Ag Oligoribonukleotide zur Beeinflussung des Haarwachstums
AU2005259799A1 (en) 2004-07-02 2006-01-12 Protiva Biotherapeutics, Inc. Immunostimulatory siRNA molecules and uses therefor
JP2008512500A (ja) 2004-09-10 2008-04-24 ソマジェニックス インコーポレーティッド ウィルス遺伝子発現を効率的に阻害する低分子干渉ｒｎａおよびその使用方法
US20060166234A1 (en) * 2004-11-22 2006-07-27 Barbara Robertson Apparatus and system having dry control gene silencing compositions
US7923207B2 (en) 2004-11-22 2011-04-12 Dharmacon, Inc. Apparatus and system having dry gene silencing pools
US7935811B2 (en) * 2004-11-22 2011-05-03 Dharmacon, Inc. Apparatus and system having dry gene silencing compositions
US20060142228A1 (en) * 2004-12-23 2006-06-29 Ambion, Inc. Methods and compositions concerning siRNA's as mediators of RNA interference
WO2006074108A2 (en) * 2004-12-30 2006-07-13 Hauser Todd M Compositions and methods for modulating gene expression using self-protected oligonucleotides
WO2006072887A1 (en) 2005-01-05 2006-07-13 Eyegate Pharma Sa Ocular iontophoresis device for delivering sirna and aptamers
DK1841793T3 (da) 2005-01-07 2010-07-19 Diadexus Inc Ovr110-antistofsammensætninger og fremgangsmåder til anvendelse deraf
JPWO2006093083A1 (ja) * 2005-03-03 2008-08-07 和光純薬工業株式会社 架橋剤、架橋法、遺伝子発現調節法及び遺伝子機能調査法
US20060223777A1 (en) * 2005-03-29 2006-10-05 Dharmacon, Inc. Highly functional short hairpin RNA
WO2006113743A2 (en) * 2005-04-18 2006-10-26 Massachusetts Institute Of Technology Compositions and methods for rna interference with sialidase expression and uses thereof
JP4967137B2 (ja) * 2005-04-18 2012-07-04 国立大学法人浜松医科大学 癌治療用組成物
EP1764107A1 (en) * 2005-09-14 2007-03-21 Gunther Hartmann Compositions comprising immunostimulatory RNA oligonucleotides and methods for producing said RNA oligonucleotides
DK2463388T3 (en) 2005-11-29 2018-02-26 Cambridge Entpr Ltd Markers for breast cancer
CA2638837A1 (en) * 2006-01-27 2007-08-02 Santaris Pharma A/S Lna modified phosphorothiolated oligonucleotides
US8329888B2 (en) 2006-03-23 2012-12-11 Santaris Pharma A/S Small internally segmented interfering RNA
GB0608838D0 (en) 2006-05-04 2006-06-14 Novartis Ag Organic compounds
US7872118B2 (en) 2006-09-08 2011-01-18 Opko Ophthalmics, Llc siRNA and methods of manufacture
US8252755B2 (en) * 2006-09-22 2012-08-28 Dharmacon, Inc. Duplex oligonucleotide complexes and methods for gene silencing by RNA interference
WO2008053487A2 (en) 2006-11-01 2008-05-08 The Medical Research Fund At The Tel-Aviv Sourasky Medical Center Adipocyte-specific constructs and methods for inhibiting platelet-type 12 lipoxygenase expression
CA2670696A1 (en) 2006-11-27 2008-06-05 Diadexus, Inc. Ovr110 antibody compositions and methods of use
PL2129680T3 (pl) 2007-03-21 2015-10-30 Brookhaven Science Ass Llc Łączone kompozycje cząsteczek antysensownych o strukturze spinki do włosów oraz sposoby modulacji ekspresji
MX2009011218A (es) 2007-04-17 2010-02-11 Baxter Int Microparticulas de acido nucleico para administracion pulmonar.
JP5296328B2 (ja) 2007-05-09 2013-09-25 独立行政法人理化学研究所 １本鎖環状ｒｎａおよびその製造方法
PL2162538T3 (pl) 2007-05-22 2016-10-31 Oligomery do zastosowań terapeutycznych
WO2009039300A2 (en) * 2007-09-18 2009-03-26 Intradigm Corporation Compositions comprising hif-1 alpha sirna and methods of use thereof
CA2704737A1 (en) * 2007-09-18 2009-09-03 Intradigm Corporation Compositions comprising k-ras sirna and methods of use
DK2195428T3 (en) 2007-09-19 2014-03-03 Applied Biosystems Llc SIRNA SEQUENCE-INDEPENDENT MODIFICATION FORMS TO REDUCE TARGET-FAILING PHENOTYPIC EFFECTS OF RNAI, AND STABILIZED FORMS THEREOF
JP5410434B2 (ja) 2007-09-28 2014-02-05 バインド セラピューティックス インコーポレイテッド ナノ粒子を用いた癌細胞の標的化
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US20100204305A1 (en) * 2007-12-11 2010-08-12 Lorus Therapeutics Inc. Small interfering rna molecules against ribonucleotide reductase and uses thereof
EP2242854A4 (en) * 2008-01-15 2012-08-15 Quark Pharmaceuticals Inc COMPOUNDS AND USES THEREOF
US8188060B2 (en) 2008-02-11 2012-05-29 Dharmacon, Inc. Duplex oligonucleotides with enhanced functionality in gene regulation
CN102016036B (zh) 2008-02-11 2015-04-08 阿克赛医药公司 经修饰的RNAi多核苷酸及其用途
WO2009114726A1 (en) * 2008-03-12 2009-09-17 Intradigm Corporation Compositions comprising notch1 sirna and methods of use thereof
US8815818B2 (en) 2008-07-18 2014-08-26 Rxi Pharmaceuticals Corporation Phagocytic cell delivery of RNAI
WO2010021718A1 (en) 2008-08-19 2010-02-25 Nektar Therapeutics Complexes of small-interfering nucleic acids
EP2165710A1 (en) 2008-09-19 2010-03-24 Institut Curie Tyrosine kinase receptor Tyro3 as a therapeutic target in the treatment of a bladder tumor
CN102405286A (zh) 2008-09-22 2012-04-04 阿克赛医药公司 减小大小的自递送RNAi化合物
US8283460B2 (en) 2008-10-15 2012-10-09 Somagenics, Inc. Short hairpin RNAs for inhibition of gene expression
US9074211B2 (en) 2008-11-19 2015-07-07 Rxi Pharmaceuticals Corporation Inhibition of MAP4K4 through RNAI
EP2370577B1 (en) 2008-12-03 2019-08-28 Arcturus Therapeutics, Inc. Usirna complexes
EP2198879A1 (en) 2008-12-11 2010-06-23 Institut Curie CD74 modulator agent for regulating dendritic cell migration and device for studying the motility capacity of a cell
EP2370175A2 (en) 2008-12-16 2011-10-05 Bristol-Myers Squibb Company Methods of inhibiting quiescent tumor proliferation
US9493774B2 (en) 2009-01-05 2016-11-15 Rxi Pharmaceuticals Corporation Inhibition of PCSK9 through RNAi
WO2010090762A1 (en) 2009-02-04 2010-08-12 Rxi Pharmaceuticals Corporation Rna duplexes with single stranded phosphorothioate nucleotide regions for additional functionality
JP6066035B2 (ja) * 2009-02-12 2017-01-25 クルナ・インコーポレーテッド グリア細胞由来神経栄養因子（ｇｄｎｆ）関連疾病の、ｇｄｎｆに対する天然アンチセンス転写物の抑制による治療
CN101525388B (zh) * 2009-02-20 2012-02-01 中国人民解放军第四军医大学 特异性双链rna结合蛋白嵌合体及其在病毒感染性疾病中的应用
EP2427180B1 (en) 2009-05-05 2016-04-13 Beeologics Inc. Prevention and treatment of nosema disease in bees
WO2010141511A2 (en) 2009-06-01 2010-12-09 Halo-Bio Rnai Therapeutics, Inc. Polynucleotides for multivalent rna interference, compositions and methods of use thereof
EP2266550A1 (en) 2009-06-15 2010-12-29 Institut Curie Antagonists of ß-catenin for preventing and/or treating neurodegenerative disorders
WO2011008730A2 (en) 2009-07-13 2011-01-20 Somagenics Inc. Chemical modification of small hairpin rnas for inhibition of gene expression
CN102822200A (zh) 2009-07-20 2012-12-12 百时美施贵宝公司 对增殖性疾病进行协同性治疗的抗ctla-4抗体与各种治疗方案的组合
US20110039789A1 (en) * 2009-08-14 2011-02-17 Institut Curie Use of Huntingtin Protein for the Diagnosis and the Treatment of Cancer
WO2011035065A1 (en) 2009-09-17 2011-03-24 Nektar Therapeutics Monoconjugated chitosans as delivery agents for small interfering nucleic acids
US20150025122A1 (en) 2009-10-12 2015-01-22 Larry J. Smith Methods and Compositions for Modulating Gene Expression Using Oligonucleotide Based Drugs Administered in vivo or in vitro
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
AU2010332881B2 (en) 2009-12-18 2015-01-22 Arrowhead Pharmaceuticals, Inc. Organic compositions to treat HSF1-related diseases
AU2011214465A1 (en) 2010-02-10 2012-08-30 Novartis Ag Methods and compounds for muscle growth
CN106074591B (zh) 2010-03-24 2020-01-14 菲奥医药公司 眼部症候中的rna干扰
RU2615143C2 (ru) 2010-03-24 2017-04-04 Адвирна Самодоставляющие PHKi соединения уменьшенного размера
US9340786B2 (en) 2010-03-24 2016-05-17 Rxi Pharmaceuticals Corporation RNA interference in dermal and fibrotic indications
EP2552460A1 (en) 2010-03-29 2013-02-06 Universite De Strasbourg Polymers for delivering molecules of interest
AU2011244335B2 (en) 2010-04-23 2015-06-04 Arrowhead Pharmaceuticals, Inc. Organic compositions to treat Beta-ENaC-related diseases
WO2011151321A1 (en) 2010-05-31 2011-12-08 Institut Curie Asf1b as a prognosis marker and therapeutic target in human cancer
EP2433644A1 (en) 2010-09-22 2012-03-28 IMBA-Institut für Molekulare Biotechnologie GmbH Breast cancer therapeutics
AR083445A1 (es) 2010-10-14 2013-02-27 Univ Mie siARN CONTRA LA FIBROSIS
EP2455456A1 (en) 2010-11-22 2012-05-23 Institut Curie Use of kinesin inhibitors in HIV infection treatment and a method for screening them
WO2012102793A2 (en) 2010-12-10 2012-08-02 Zirus, Inc. Mammalian genes involved in toxicity and infection
WO2012119764A1 (en) 2011-03-08 2012-09-13 Società Bulloneria Europea S.B.E. Spa A high load flanged fastener to be installed by tensioning tools
JP2014510526A (ja) 2011-03-15 2014-05-01 ユニヴァーシティー オブ ユタ リサーチ ファウンデーション 血管関連黄斑症およびその症状の診断および治療方法
EP2751272A2 (en) 2011-09-02 2014-07-09 Novartis AG Organic compositions to treat hsf1-related diseases
US10023862B2 (en) 2012-01-09 2018-07-17 Arrowhead Pharmaceuticals, Inc. Organic compositions to treat beta-catenin-related diseases
KR101371696B1 (ko) 2012-02-02 2014-03-07 세종대학교산학협력단 벼도열병 발병인자의 스크리닝 방법
EP3272868A1 (en) 2012-05-02 2018-01-24 Arrowhead Pharmaceuticals, Inc. Organic compositions to treat kras-related diseases
WO2014018375A1 (en) 2012-07-23 2014-01-30 Xenon Pharmaceuticals Inc. Cyp8b1 and uses thereof in therapeutic and diagnostic methods
EP2700949A1 (en) 2012-08-24 2014-02-26 IMG Institut für medizinische Genomforschung Planungsgesellschaft M.B.H. Use of biliverdin reductase proteins as cancer marker
EP2961843A2 (en) 2013-02-28 2016-01-06 Arrowhead Research Corporation Organic compositions to treat epas1-related diseases
WO2014135655A1 (en) 2013-03-06 2014-09-12 Institut Curie Compositions and methods for treating muscle-invasive bladder cancer
CN105518136A (zh) 2013-07-10 2016-04-20 巴斯夫欧洲公司 用于通过抑制CYP51基因表达来控制致植物病真菌和卵菌的RNAi
RU2703498C2 (ru) 2013-07-19 2019-10-17 Монсанто Текнолоджи Ллс Композиции и способы борьбы с leptinotarsa
EP3030171B1 (en) 2013-08-08 2017-11-01 Global Bio Therapeutics, Inc. Injection device for minimally invasive procedures
WO2015051135A2 (en) 2013-10-04 2015-04-09 Novartis Ag Organic compositions to treat hepcidin-related diseases
CN113151180A (zh) 2013-12-02 2021-07-23 菲奥医药公司 癌症的免疫治疗
ES2796090T3 (es) 2014-02-10 2020-11-25 Inst Curie Uso de moduladores MCOLN-1 para regular la migración celular
US9856475B2 (en) 2014-03-25 2018-01-02 Arcturus Therapeutics, Inc. Formulations for treating amyloidosis
EP3122365B1 (en) 2014-03-25 2023-05-03 Arcturus Therapeutics, Inc. Transthyretin allele selective una oligomers for gene silencing
CA2946719C (en) 2014-03-25 2023-09-26 Arcturus Therapeutics, Inc. Una oligomers having reduced off-target effects in gene silencing
SG11201607772WA (en) 2014-03-31 2016-10-28 Debiopharm Int Sa Fgfr fusions
EP3420809A1 (en) 2014-04-01 2019-01-02 Monsanto Technology LLC Compositions and methods for controlling insect pests
US11279934B2 (en) 2014-04-28 2022-03-22 Phio Pharmaceuticals Corp. Methods for treating cancer using nucleic acids targeting MDM2 or MYCN
WO2016011123A1 (en) 2014-07-16 2016-01-21 Arrowhead Research Corporation Organic compositions to treat apoc3-related diseases
US10378012B2 (en) 2014-07-29 2019-08-13 Monsanto Technology Llc Compositions and methods for controlling insect pests
CN107073294A (zh) 2014-09-05 2017-08-18 阿克赛医药公司 使用靶向tyr或mmp1的核酸治疗老化和皮肤病症的方法
RU2017110868A (ru) 2014-09-25 2018-10-25 Колд Спринг Харбор Лаборатори Лечение синдрома ретта
RU2723049C2 (ru) 2015-01-22 2020-06-08 Монсанто Текнолоджи Ллс Композиции и способы борьбы с leptinotarsa
WO2016161299A1 (en) 2015-04-01 2016-10-06 Arcturus Therapeutics, Inc. Therapeutic una oligomers and uses thereof
WO2017007825A1 (en) 2015-07-06 2017-01-12 Rxi Pharmaceuticals Corporation Methods for treating neurological disorders using a synergistic small molecule and nucleic acids therapeutic approach
WO2017007813A1 (en) 2015-07-06 2017-01-12 Rxi Pharmaceuticals Corporation Nucleic acid molecules targeting superoxide dismutase 1 (sod1)
WO2017015671A1 (en) 2015-07-23 2017-01-26 Arcturus Therapeutics, Inc. Compositions for treating amyloidosis
WO2017013270A1 (en) 2015-07-23 2017-01-26 Universite De Strasbourg Use of leptin signaling inhibitor for protecting kidneys from patients having ciliopathy
WO2017035278A1 (en) 2015-08-24 2017-03-02 Halo-Bio Rnai Therapeutics, Inc. Polynucleotide nanoparticles for the modulation of gene expression and uses thereof
WO2017058944A1 (en) 2015-09-29 2017-04-06 Amgen Inc. Asgr inhibitors
CN109563509B (zh) 2015-10-19 2022-08-09 菲奥医药公司 靶向长非编码rna的减小尺寸的自递送核酸化合物
MA45469A (fr) 2016-04-01 2019-02-06 Avidity Biosciences Llc Acides nucléiques de bêta-caténine et leurs utilisations
MA45349A (fr) 2016-04-01 2019-02-06 Avidity Biosciences Llc Acides nucléiques egfr et leurs utilisations
MA45470A (fr) 2016-04-01 2019-02-06 Avidity Biosciences Llc Acides nucléiques kras et leurs utilisations
MA45328A (fr) 2016-04-01 2019-02-06 Avidity Biosciences Llc Compositions acide nucléique-polypeptide et utilisations de celles-ci
EP3555138A1 (en) 2016-12-16 2019-10-23 Université de Bordeaux Mmp9 inhibitors and uses thereof in the prevention or treatment of a depigmenting disorder
US12150978B2 (en) 2017-06-15 2024-11-26 Cancer Advances Inc. Compositions and methods for preventing tumors and cancer
EP3624841A4 (en) 2017-06-15 2021-01-27 Cancer Advances, Inc., COMPOSITIONS AND METHODS FOR INDUCING HUMORAL AND CELLULAR IMMUNITIES AGAINST TUMORS AND CANCER
CN111065731B (zh) 2017-06-16 2024-03-12 Imba-莫利库尔生物技术研究所 血管类器官、产生和使用所述类器官的方法
CR20200108A (es) 2017-09-11 2020-06-28 Arrowhead Pharmaceuticals Inc Rnai agents and compositions for inhibiting expression of apolipoprotein c-iii (apoc3)
IL273245B2 (en) 2017-09-15 2025-03-01 Commw Scient Ind Res Org RNA molecules
WO2019113393A1 (en) 2017-12-06 2019-06-13 Avidity Biosciences Llc Compositions and methods of treating muscle atrophy and myotonic dystrophy
EP3801025A4 (en) 2018-05-24 2022-03-09 Sirnaomics, Inc. Composition and methods of controllable co-coupling polypeptide nanoparticle delivery system for nucleic acid therapeutics
JP7588074B2 (ja) 2018-12-27 2024-11-21 サーナオミクス インコーポレイテッド がんを治療するために、免疫チェックポイント阻害剤と組み合わせて送達されるｓｉＲＮＡを使用したＴＧＦ－ベータ１およびＣｏｘ２のサイレンシング
IT201900005918A1 (it) 2019-04-16 2020-10-16 Pastificio Rana Spa Macchina per cuocere una dose di pasta in un contenitore
TW202144572A (zh) 2020-03-19 2021-12-01 美商亞維代堤生物科學公司 治療臉肩胛肱骨肌肉失養症之組合物及方法
WO2025157611A1 (en) 2024-01-24 2025-07-31 Basf Se Mixtures comprising rna and fungicides to control phytopathogenic fungi
WO2025157614A1 (en) 2024-01-24 2025-07-31 Basf Se Mixtures comprising rna and fungicides to control phytopathogenic fungi
WO2025157612A1 (en) 2024-01-24 2025-07-31 Basf Se Mixtures comprising rna to control phytopathogenic fungi.

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992019732A1 (fr) * 1991-04-25 1992-11-12 Genset Oligonucleotides fermes, antisens et sens, et leurs applications
WO1998005770A2 (de) * 1996-08-07 1998-02-12 Deutches Krebsforschungszentrum Stiftung Des Öffentlichen Rechts Anti-sinn-rna mit sekundärstruktur
WO1999032619A1 (en) * 1997-12-23 1999-07-01 The Carnegie Institution Of Washington Genetic inhibition by double-stranded rna

Family Cites Families (157)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5208149A (en) 1983-10-20 1993-05-04 The Research Foundation Of State University Of New York Nucleic acid constructs containing stable stem and loop structures
US5190931A (en) 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
WO1987000201A1 (en) 1985-07-05 1987-01-15 Whitehead Institute For Biomedical Research Epithelial cells expressing foreign genetic material
US4980286A (en) 1985-07-05 1990-12-25 Whitehead Institute For Biomedical Research In vivo introduction and expression of foreign genetic material in epithelial cells
US5107065A (en) * 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5712257A (en) 1987-08-12 1998-01-27 Hem Research, Inc. Topically active compositions of mismatched dsRNAs
EP0378576B1 (en) 1987-09-11 1995-01-18 Whitehead Institute For Biomedical Research Transduced fibroblasts and uses therefor
EP0391960B1 (en) 1987-12-11 1994-08-17 Whitehead Institute For Biomedical Research Genetic modification of endothelial cells
US5254678A (en) 1987-12-15 1993-10-19 Gene Shears Pty. Limited Ribozymes
US5100087A (en) * 1989-03-06 1992-03-31 Ashby Stephen B Fastening device for container liners
EP0732397A3 (en) 1988-02-05 1996-10-23 Whitehead Institute For Biomedical Research Modified hepatocytes and uses therefor
US5866701A (en) 1988-09-20 1999-02-02 The Board Of Regents For Northern Illinois University Of Dekalb HIV targeted hairpin ribozymes
ATE174514T1 (de) 1989-01-23 1999-01-15 Chiron Corp Rekombinante zellen für therapien von infektionen und hyperprolieferative störungen und deren herstellung
US5328470A (en) * 1989-03-31 1994-07-12 The Regents Of The University Of Michigan Treatment of diseases by site-specific instillation of cells or site-specific transformation of cells and kits therefor
US5112734A (en) 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
US5225347A (en) 1989-09-25 1993-07-06 Innovir Laboratories, Inc. Therapeutic ribozyme compositions and expression vectors
US5212295A (en) 1990-01-11 1993-05-18 Isis Pharmaceuticals Monomers for preparation of oligonucleotides having chiral phosphorus linkages
US5587361A (en) * 1991-10-15 1996-12-24 Isis Pharmaceuticals, Inc. Oligonucleotides having phosphorothioate linkages of high chiral purity
US6245560B1 (en) 1990-01-18 2001-06-12 The United States Of America As Represented By The Department Of Health And Human Services Vector with multiple target response elements affecting gene expression
US5683867A (en) * 1990-06-11 1997-11-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: blended SELEX
US5246921A (en) 1990-06-26 1993-09-21 The Wistar Institute Of Anatomy And Biology Method for treating leukemias
WO1992001806A1 (en) * 1990-07-16 1992-02-06 The Public Health Research Institute Of The City Of New York, Inc. Selection of ribozymes that efficiently cleave target rna
WO1992007573A1 (en) 1990-10-31 1992-05-14 Somatix Therapy Corporation Genetic modification of endothelial cells
US5238470A (en) * 1992-02-21 1993-08-24 Westavco Corporation Emission control device
ATE212998T1 (de) 1992-03-05 2002-02-15 Isis Pharmaceuticals Inc Kovalent vernetzte oligonukleotide
ES2170065T3 (es) * 1992-03-16 2002-08-01 Isis Pharmaceuticals Inc Modulacion de la proteina quinasa c por oligonucleotidos.
US5496698A (en) 1992-08-26 1996-03-05 Ribozyme Pharmaceuticals, Inc. Method of isolating ribozyme targets
US5525468A (en) 1992-05-14 1996-06-11 Ribozyme Pharmaceuticals, Inc. Assay for Ribozyme target site
JPH08501928A (ja) 1992-07-02 1996-03-05 ハイブライドン インコーポレイテッド 治療剤としての自己安定化オリゴヌクレオチド
US6423489B1 (en) 1992-09-10 2002-07-23 Isis Pharmaceuticals, Inc. Compositions and methods for treatment of Hepatitis C virus-associated diseases
WO1994010301A1 (en) 1992-11-03 1994-05-11 Gene Shears Pty. Limited TNF-α RIBOZYMES AND DEGRADATION RESISTANT mRNA DERIVATIVES LINKED TO TNF-α RIBOZYMES
US5864028A (en) 1992-11-03 1999-01-26 Gene Shears Pty. Limited Degradation resistant mRNA derivatives linked to TNF-α ribozymes
JPH08506724A (ja) 1992-12-04 1996-07-23 アポロン・インコーポレーテッド 白血病治療のための化合物および方法
US5811300A (en) * 1992-12-07 1998-09-22 Ribozyme Pharmaceuticals, Inc. TNF-α ribozymes
US5574142A (en) * 1992-12-15 1996-11-12 Microprobe Corporation Peptide linkers for improved oligonucleotide delivery
US5639655A (en) * 1993-01-19 1997-06-17 Ribozyme Pharmaceuticals, Inc. PML-RARA targeted ribozymes
FR2703053B1 (fr) * 1993-03-26 1995-06-16 Genset Sa Oligonucleotides agrafes et semi-agrafes, procede de preparation et applications .
NZ267797A (en) 1993-05-17 1997-09-22 Univ California Retroviral vector comprising a ribozyme capable of cleaving a hiv nucleic acid capable
US5635385A (en) 1993-09-15 1997-06-03 Temple University-Of The Commonwealth System Of Higher Education Multi-unit ribozyme inhibition of oncogene gene expression
DE4335025A1 (de) * 1993-10-14 1995-04-20 Boehringer Ingelheim Int Endosomolytisch wirksame Partikel
US5624803A (en) * 1993-10-14 1997-04-29 The Regents Of The University Of California In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom
US5801154A (en) * 1993-10-18 1998-09-01 Isis Pharmaceuticals, Inc. Antisense oligonucleotide modulation of multidrug resistance-associated protein
US5908779A (en) * 1993-12-01 1999-06-01 University Of Connecticut Targeted RNA degradation using nuclear antisense RNA
US5755269A (en) * 1993-12-09 1998-05-26 Ciba Corning Diagnostics Corp. Fluid delivery system
US6054299A (en) * 1994-04-29 2000-04-25 Conrad; Charles A. Stem-loop cloning vector and method
EP0797663A4 (en) 1994-11-29 1999-11-24 Univ Queensland SOX-9 GENE AND PROTEIN CODED BY AND THEIR APPLICATION FOR BONE AND CARTILAGINOUS REGENERATION
US6071890A (en) 1994-12-09 2000-06-06 Genzyme Corporation Organ-specific targeting of cationic amphiphile/DNA complexes for gene therapy
US5683873A (en) 1995-01-13 1997-11-04 Innovir Laboratories, Inc. EGS-mediated inactivation of target RNA
US6166197A (en) 1995-03-06 2000-12-26 Isis Pharmaceuticals, Inc. Oligomeric compounds having pyrimidine nucleotide (S) with 2'and 5 substitutions
US5674683A (en) * 1995-03-21 1997-10-07 Research Corporation Technologies, Inc. Stem-loop and circular oligonucleotides and method of using
US5739271A (en) 1995-06-07 1998-04-14 Gen-Probe Incorporated Thiocationic lipids
AU727531B2 (en) * 1995-07-25 2000-12-14 Crucell Holland B.V. Methods and means for targeted gene delivery
US6482803B1 (en) 1995-09-01 2002-11-19 Board Of Regents, The University Of Texas System Modification of mutated P53 gene in tumors by retroviral delivery of ribozyme A
US6346398B1 (en) 1995-10-26 2002-02-12 Ribozyme Pharmaceuticals, Inc. Method and reagent for the treatment of diseases or conditions related to levels of vascular endothelial growth factor receptor
US5824519A (en) 1995-11-08 1998-10-20 Medical University Of South Carolina Tissue-specific and target RNA-specific ribozymes
US5998203A (en) * 1996-04-16 1999-12-07 Ribozyme Pharmaceuticals, Inc. Enzymatic nucleic acids containing 5'-and/or 3'-cap structures
WO1997026270A2 (en) * 1996-01-16 1997-07-24 Ribozyme Pharmaceuticals, Inc. Synthesis of methoxy nucleosides and enzymatic nucleic acid molecules
US5891717A (en) 1996-01-19 1999-04-06 Betagene, Inc. Methods and compositions for inhibiting hexokinase
US5854067A (en) * 1996-01-19 1998-12-29 Board Of Regents, The University Of Texas System Hexokinase inhibitors
US6099823A (en) 1996-02-16 2000-08-08 Millennium Pharmaceuticals, Inc. Compositions and methods for the treatment and diagnosis of cardiovascular disease
US5731181A (en) * 1996-06-17 1998-03-24 Thomas Jefferson University Chimeric mutational vectors having non-natural nucleotides
DE19618797C2 (de) 1996-05-10 2000-03-23 Bertling Wolf Vehikel zum Transport molekularer Substanz
WO1997044348A1 (en) * 1996-05-17 1997-11-27 Thomas Jefferson University Ribozyme-mediated gene replacement
US5898031A (en) 1996-06-06 1999-04-27 Isis Pharmaceuticals, Inc. Oligoribonucleotides for cleaving RNA
US5939262A (en) 1996-07-03 1999-08-17 Ambion, Inc. Ribonuclease resistant RNA preparation and utilization
US6255071B1 (en) 1996-09-20 2001-07-03 Cold Spring Harbor Laboratory Mammalian viral vectors and their uses
US5814500A (en) 1996-10-31 1998-09-29 The Johns Hopkins University School Of Medicine Delivery construct for antisense nucleic acids and methods of use
US5968737A (en) 1996-11-12 1999-10-19 The University Of Mississippi Method of identifying inhibitors of glutathione S-transferase (GST) gene expression
US6057156A (en) * 1997-01-31 2000-05-02 Robozyme Pharmaceuticals, Inc. Enzymatic nucleic acid treatment of diseases or conditions related to levels of epidermal growth factor receptors
US6100444A (en) 1997-02-11 2000-08-08 University Of Rochester Medical Center Prostate specific regulatory nucleic acid sequences and transgenic non-human animals expressing prostate specific antigen
US6127533A (en) 1997-02-14 2000-10-03 Isis Pharmaceuticals, Inc. 2'-O-aminooxy-modified oligonucleotides
NZ500427A (en) * 1997-04-21 2002-12-20 Univ Florida The use of ribozyme treatment for retinal diseases
GB9710475D0 (en) 1997-05-21 1997-07-16 Zeneca Ltd Gene silencing
US6183959B1 (en) 1997-07-03 2001-02-06 Ribozyme Pharmaceuticals, Inc. Method for target site selection and discovery
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
GB9720148D0 (en) 1997-09-22 1997-11-26 Innes John Centre Innov Ltd Gene silencing materials and methods
JP2001517461A (ja) 1997-09-26 2001-10-09 ジョージタウン ユニバーシティ リボザイムを用いたｆｇｆ結合蛋白質の阻害
US6355415B1 (en) 1997-09-29 2002-03-12 Ohio University Compositions and methods for the use of ribozymes to determine gene function
US6087164A (en) 1997-10-03 2000-07-11 Yissum Research Development Company Of The Hebrew University Of Jerusalem Methods and compositions for inducing tumor-specific cytotoxicity
US6087172A (en) 1997-10-31 2000-07-11 Hisamitsu Pharmaceutical Co., Inc. Ribozymes targeted to human IL-15 mRNA
US6100087A (en) * 1998-03-11 2000-08-08 City Of Hope Ribozymes targeted to human CCR5 mRNA
CZ295108B6 (cs) * 1998-03-20 2005-05-18 Benitec Australia Ltd Syntetický gen obsahující dispergovanou nebo cizorodou deoxyribonukleovou molekulu a genový konstrukt obsahující tento syntetický gen
DE69943389D1 (de) * 1998-04-08 2011-06-09 Commw Scient Ind Res Org Verfahren und mittel zum erhalt von veränderten phänotypen
EP1071753A2 (en) 1998-04-20 2001-01-31 Ribozyme Pharmaceuticals, Inc. Nucleic acid molecules with novel chemical compositions capable of modulating gene expression
US6010907A (en) 1998-05-12 2000-01-04 Kimeragen, Inc. Eukaryotic use of non-chimeric mutational vectors
AR020078A1 (es) * 1998-05-26 2002-04-10 Syngenta Participations Ag Metodo para alterar la expresion de un gen objetivo en una celula de planta
GB9827152D0 (en) 1998-07-03 1999-02-03 Devgen Nv Characterisation of gene function using double stranded rna inhibition
US6271358B1 (en) 1998-07-27 2001-08-07 Isis Pharmaceuticals, Inc. RNA targeted 2′-modified oligonucleotides that are conformationally preorganized
US6486299B1 (en) * 1998-09-28 2002-11-26 Curagen Corporation Genes and proteins predictive and therapeutic for stroke, hypertension, diabetes and obesity
MXPA01003643A (es) 1998-10-09 2003-07-21 Ingene Inc Produccion de adnss in vivo.
AU6430599A (en) 1998-10-09 2000-05-01 Cytogenix, Inc. Enzymatic synthesis of ssdna
CA2361201A1 (en) 1999-01-28 2000-08-03 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna
US6200924B1 (en) 1999-01-29 2001-03-13 E. I. Du Pont De Nemours And Company Porous highly fluorinated acidic polymer catalyst
DE19956568A1 (de) 1999-01-30 2000-08-17 Roland Kreutzer Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
BR0009884A (pt) * 1999-04-21 2002-01-08 American Home Prod Processos e composições para a inibição da função das sequências de polinucleotìdeos
AU4563300A (en) 1999-05-10 2000-11-21 Syngenta Participations Ag Regulation of viral gene expression
US6569623B1 (en) 1999-09-08 2003-05-27 Ramot University Authority For Applied Research & Industrial Development Ltd. Genetic screening methods
US6861220B2 (en) * 1999-09-08 2005-03-01 Ramot University Authority For Applied Research & Industrial Development Ltd Genetic screening methods
JP2003516124A (ja) 1999-10-15 2003-05-13 ユニバーシティー オブ マサチューセッツ 標的とした遺伝的干渉の手段としてのｒｎａ干渉経路遺伝子
GB9927444D0 (en) * 1999-11-19 2000-01-19 Cancer Res Campaign Tech Inhibiting gene expression
DE10160151A1 (de) 2001-01-09 2003-06-26 Ribopharma Ag Verfahren zur Hemmung der Expression eines vorgegebenen Zielgens
DE10100586C1 (de) 2001-01-09 2002-04-11 Ribopharma Ag Verfahren zur Hemmung der Expression eines Ziegens
US7829693B2 (en) 1999-11-24 2010-11-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
RU2164944C1 (ru) 1999-12-09 2001-04-10 Институт молекулярной биологии им. В.А. Энгельгардта РАН Способ изменения генетических свойств организма
GB9930691D0 (en) 1999-12-24 2000-02-16 Devgen Nv Improvements relating to double-stranded RNA inhibition
AU2001245793A1 (en) 2000-03-16 2001-09-24 Cold Spring Harbor Laboratory Methods and compositions for rna interference
AU2001240375A1 (en) 2000-03-17 2001-10-03 Benitec Australia Limited Genetic silencing
EP1309726B2 (en) 2000-03-30 2018-10-03 Whitehead Institute For Biomedical Research Rna sequence-specific mediators of rna interference
EP1290161B1 (en) 2000-05-30 2011-06-22 Johnson & Johnson Research Pty Limited METHODS FOR MEDIATING GENE SUPPRESION BY USING FACTORS THAT ENHANCE RNAi
CA2456008A1 (en) 2000-08-19 2002-02-28 Axordia Limited Stem cell differentiation
US20030190635A1 (en) 2002-02-20 2003-10-09 Mcswiggen James A. RNA interference mediated treatment of Alzheimer's disease using short interfering RNA
AU2001296333A1 (en) 2000-09-26 2002-04-08 The Burnham Institute Paad domain-containing polypeptides, encoding nucleic acids, and methods of use
WO2002068637A2 (en) 2000-10-20 2002-09-06 Ribozyme Pharmaceuticals, Inc. Nucleic acid-based treatment of diseases or conditions related to west nile virus infection
US20020173478A1 (en) 2000-11-14 2002-11-21 The Trustees Of The University Of Pennsylvania Post-transcriptional gene silencing by RNAi in mammalian cells
US20040259247A1 (en) 2000-12-01 2004-12-23 Thomas Tuschl Rna interference mediating small rna molecules
WO2002061034A2 (en) 2000-12-08 2002-08-08 Invitrogen Corporation Compositions and methods for rapidly generating recombinant nucleic acid molecules
US8546143B2 (en) 2001-01-09 2013-10-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
WO2003035869A1 (de) 2001-10-26 2003-05-01 Ribopharma Ag Verwendung einer doppelsträngigen ribonukleinsäure zur gezielten hemmung der expression eines vorgegebenen zielgens
DE10100588A1 (de) 2001-01-09 2002-07-18 Ribopharma Ag Verfahren zur Hemmung der Expression eines Zielgens
US6335415B1 (en) * 2001-01-30 2002-01-01 Council Of Scientific & Industrial Research Process for the preparation of a polyester
AU2002256990A1 (en) 2001-02-07 2002-09-24 The Burnham Institute Apoptosis modulator bcl-b and methods for making and using same
GB0104948D0 (en) 2001-02-28 2001-04-18 Novartis Res Foundation Novel methods
AU2002258477A1 (en) 2001-03-08 2002-09-24 Advanced Cell Technology, Inc. Use of rna interference for the creation of lineage specific es and other undifferentiated cells and production of differentiated cells in vitro by co-culture
US6787063B2 (en) 2001-03-12 2004-09-07 Seiko Epson Corporation Compositions, methods for producing films, functional elements, methods for producing functional elements, methods for producing electro-optical devices and methods for producing electronic apparatus
EP1386004A4 (en) * 2001-04-05 2005-02-16 Ribozyme Pharm Inc MODULATION OF GENE EXPRESSION ASSOCIATED WITH INFLAMMATORY PROLIFERATION AND GROWTH OF NEURITIES BY METHODS INVOLVING NUCLEIC ACID
WO2003070972A2 (en) 2002-02-20 2003-08-28 Sirna Therapeutics Inc. RNA INTERFERENCE MEDIATED INHIBITION OF CHROMOSOME TRANSLOCATION GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
JP4210737B2 (ja) 2001-07-12 2009-01-21 ユニバーシティー オブ マサチューセッツ 遺伝子サイレンシングを仲介する低分子干渉リボ核酸のインビボにおける製造方法
GB0118223D0 (en) 2001-07-26 2001-09-19 Univ Sheffield Stem loop RNA
WO2003012052A2 (en) 2001-07-30 2003-02-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Specific inhibition of gene expression by small double stranded rnas
WO2003016572A1 (en) 2001-08-17 2003-02-27 Eli Lilly And Company Oligonucleotide therapeutics for treating hepatitis c virus infections
US7101995B2 (en) 2001-08-27 2006-09-05 Mirus Bio Corporation Compositions and processes using siRNA, amphipathic compounds and polycations
US20030198627A1 (en) 2001-09-01 2003-10-23 Gert-Jan Arts siRNA knockout assay method and constructs
DE10163098B4 (de) 2001-10-12 2005-06-02 Alnylam Europe Ag Verfahren zur Hemmung der Replikation von Viren
US7745418B2 (en) 2001-10-12 2010-06-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting viral replication
DE10230997A1 (de) 2001-10-26 2003-07-17 Ribopharma Ag Medikament zur Erhöhung der Wirksamkeit eines Rezeptor-vermittelt Apoptose in Tumorzellen auslösenden Arzneimittels
JP2005506087A (ja) 2001-10-26 2005-03-03 リボファーマ アーゲー プラス鎖ｒｎａウイルスによる感染症を処置するための２本鎖リボ核酸の使用
WO2003035870A1 (de) 2001-10-26 2003-05-01 Ribopharma Ag Medikament zur behandlung eines pankreaskarzinoms
JP2005512976A (ja) 2001-10-26 2005-05-12 リボファーマ アーゲー Ｒｎａ干渉により線維化疾患を処置するための医薬
DE10230996A1 (de) 2001-10-26 2003-07-17 Ribopharma Ag Medikament zur Behandlung eines Pankreaskarzinoms
WO2004033620A2 (en) * 2001-11-02 2004-04-22 Insert Therapeutics, Inc. Methods and compositions for therapeutic use of rna interference
FR2832154B1 (fr) 2001-11-09 2007-03-16 Centre Nat Rech Scient Oligonucleotides inhibiteurs et leur utilisation pour reprimer specifiquement un gene
AU2002356898A1 (en) 2001-11-15 2003-06-10 Tularik Inc. Gene amplification and overexpression in cancer
DE10202419A1 (de) 2002-01-22 2003-08-07 Ribopharma Ag Verfahren zur Hemmung der Expression eines durch eine Chromosomen-Aberration entstandenen Zielgens
EP1442143A4 (en) 2002-02-20 2005-02-16 Sirna Therapeutics Inc INHIBITION OF BCL2 GENE EXPRESSION MEDIATED BY RNA INTERFERENCE USING siNA (SHORT INTERFERING NUCLEIC ACID)
JP2005517427A (ja) 2002-02-20 2005-06-16 サーナ・セラピューティクス・インコーポレイテッド 短干渉核酸（ｓｉＮＡ）を用いるＣ型肝炎ウイルス（ＨＣＶ）遺伝子発現のＲＮＡ干渉媒介性阻害
AU2003207708A1 (en) 2002-02-20 2003-09-09 Sirna Therapeutics, Inc. Rna interference mediated inhibition of map kinase genes
WO2003070283A2 (en) 2002-02-22 2003-08-28 Klaus Strebhardt Agent for inhibiting development or progress of proliferative diseases and especially cancer diseases and pharmaceutical composition containing said agent
US20030180756A1 (en) 2002-03-21 2003-09-25 Yang Shi Compositions and methods for suppressing eukaryotic gene expression
DE10230966A1 (de) 2002-07-10 2004-01-22 Ina-Schaeffler Kg Elektromagnetisches Hydraulikventil, insbesondere Proportionalventil zur Steuerung einer Vorrichtung zur Drehwinkelverstellung einer Nockenwelle gegenüber einer Kurbelwelle einer Brennkraftmaschine
PT1527176E (pt) 2002-08-05 2007-04-30 Atugen Ag Novas formas de muléculas de arn de interferência
AU2003273336A1 (en) 2002-09-18 2004-04-08 Isis Pharmaceuticals, Inc. Efficient reduction of target rna's by single- and double-stranded oligomeric compounds
DK2284266T3 (da) 2002-11-14 2014-01-13 Thermo Fisher Scient Biosciences Inc sIRNA-MOLEKYLE MOD TP53
DE10302421A1 (de) 2003-01-21 2004-07-29 Ribopharma Ag Doppelsträngige Ribonukleinsäure mit verbesserter Wirksamkeit
GB0317988D0 (en) 2003-07-31 2003-09-03 Milner Jo Splicing variants
NZ556097A (en) * 2005-01-07 2009-12-24 Alnylam Pharmaceuticals Inc Rnai modulation of RSV and therapeutic uses thereof
EA014886B1 (ru) 2006-03-31 2011-02-28 Элнилэм Фармасьютикалз, Инк. КОМПОЗИЦИИ И СПОСОБЫ ИНГИБИРОВАНИЯ ЭКСПРЕССИИ ГЕНА Eg5

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992019732A1 (fr) * 1991-04-25 1992-11-12 Genset Oligonucleotides fermes, antisens et sens, et leurs applications
WO1998005770A2 (de) * 1996-08-07 1998-02-12 Deutches Krebsforschungszentrum Stiftung Des Öffentlichen Rechts Anti-sinn-rna mit sekundärstruktur
WO1999032619A1 (en) * 1997-12-23 1999-07-01 The Carnegie Institution Of Washington Genetic inhibition by double-stranded rna

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MADHUR K. ET AL.: "Antisense RNA : function and fate of duplex RNA in cells of higher eukaryotes.", MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, vol. 62, December 1998 (1998-12-01), pages 1415 - 1434, XP000909741 *
UHLMANN E ET AL: "ANTISENSE OLIGONUCLEOTIDES: A NEW THERAPEUTIC PRINCIPLE", CHEMICAL REVIEWS,US,AMERICAN CHEMICAL SOCIETY. EASTON, vol. 90, no. 4, 1 June 1990 (1990-06-01), pages 543 - 584, XP000141412, ISSN: 0009-2665 *

Cited By (920)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9096636B2 (en) 1996-06-06 2015-08-04 Isis Pharmaceuticals, Inc. Chimeric oligomeric compounds and their use in gene modulation
US7538095B2 (en) 1997-12-23 2009-05-26 The Carnegie Institution Of Washington Genetic inhibition by double-stranded RNA
US9029527B2 (en) 1998-03-20 2015-05-12 Commonwealth Scientific And Industrial Research Organisation Synthetic genes and genetic constructs
US9963698B2 (en) 1998-03-20 2018-05-08 Commonwealth Scientific And Industrial Research Organisation Control of gene expression
DE10066235C5 (de) 1999-01-30 2014-08-21 Alnylam Europe Ag Verfahren und Medikament zur Hemmung der Expression eines vorgegebenen Gens
US9133454B2 (en) 1999-01-30 2015-09-15 Alnylam Pharmaceuticals, Inc. Method and medicament for inhibiting the expression of a given gene
US9902955B2 (en) 1999-01-30 2018-02-27 Alnylam Pharmaceuticals, Inc. Method and medicament for inhibiting the expression of a given gene
US8367630B2 (en) 1999-03-17 2013-02-05 The University Of North Carolina At Chapel Hill Method for inhibiting expression of a protein in a hepatocyte
US10190127B2 (en) 1999-08-13 2019-01-29 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US9708621B2 (en) 1999-08-13 2017-07-18 Commonwealth Scientific And Industrial Research Organisation Methods and means for obtaining modified phenotypes
US7531718B2 (en) 1999-08-26 2009-05-12 Monsanto Technology, L.L.C. Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US7563949B2 (en) 1999-08-26 2009-07-21 Monsanto Technology Llc Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US7067722B2 (en) 1999-08-26 2006-06-27 Monsanto Technology Llc Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US7256329B2 (en) 1999-08-26 2007-08-14 Calgene Llc Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US8097778B2 (en) 1999-08-26 2012-01-17 Monsanto Company Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids
US7148336B2 (en) 1999-08-26 2006-12-12 Calgene Llc Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acid levels
JP2012085641A (ja) * 1999-11-19 2012-05-10 Cancer Research Technology Ltd Ｄｓｒｎａによる遺伝子発現の阻害
JP2015109847A (ja) * 1999-11-19 2015-06-18 キャンサー・リサーチ・テクノロジー・リミテッドＣａｎ Ｄｓｒｎａによる遺伝子発現の阻害
US7829693B2 (en) 1999-11-24 2010-11-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US7732417B2 (en) 2000-03-16 2010-06-08 Cold Spring Harbor Laboratory Methods and compositions for RNA interference using recombinant Dicer and Argonaut
US8153776B2 (en) 2000-03-16 2012-04-10 Cold Spring Harbor Laboratory Methods and compositions for RNA interference
US8383599B2 (en) 2000-03-16 2013-02-26 Cold Spring Harbor Laboratory Methods and compositions for RNA interference
US8202846B2 (en) 2000-03-16 2012-06-19 Cold Spring Harbor Laboratory Methods and compositions for RNA interference
JP2003529374A (ja) * 2000-03-30 2003-10-07 ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ Ｒｎａ干渉のｒｎａ配列特異的メディエータ
EP2796553A1 (en) * 2000-03-30 2014-10-29 Whitehead Institute for Biomedical Research RNA sequence-specific mediators of RNA interference
EP2361981A1 (en) * 2000-03-30 2011-08-31 The Whitehead Institute for Biomedical Research RNA sequence-specific mediators of RNA interference
US9012138B2 (en) 2000-03-30 2015-04-21 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA sequence-specific mediators of RNA interference
US9012621B2 (en) 2000-03-30 2015-04-21 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA sequence-specific mediators of RNA interference
US8632997B2 (en) 2000-03-30 2014-01-21 University Of Massachusetts RNA sequence-specific mediators of RNA interference
JP2012050449A (ja) * 2000-03-30 2012-03-15 Whitehead Inst For Biomedical Res Ｒｎａ干渉のｒｎａ配列特異的メディエータ
US8552171B2 (en) 2000-03-30 2013-10-08 University Of Massachusetts RNA sequence-specific mediators of RNA interference
EP2028278A1 (en) * 2000-03-30 2009-02-25 Whitehead Institute For Biomedical Research RNA sequence-specific mediators of RNA interference
EP2361981B2 (en) † 2000-03-30 2019-01-23 The Whitehead Institute for Biomedical Research RNA sequence-specific mediators of RNA interference
US8742092B2 (en) 2000-03-30 2014-06-03 University Of Massachusetts RNA sequence-specific mediators of RNA interference
US8394628B2 (en) 2000-03-30 2013-03-12 University Of Massachusetts RNA sequence-specific mediators of RNA interference
US9193753B2 (en) 2000-03-30 2015-11-24 University Of Massachusetts RNA sequence-specific mediators of RNA interference
EP2360253A1 (en) * 2000-03-30 2011-08-24 The Whitehead Institute for Biomedical Research RNA sequence-specific mediators of RNA interference
EP2345742A1 (en) * 2000-03-30 2011-07-20 The Whitehead Institute for Biomedical Research RNA sequence-specific mediators of RNA interference
US8790922B2 (en) 2000-03-30 2014-07-29 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA sequence-specific mediators of RNA interference
US10472625B2 (en) 2000-03-30 2019-11-12 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA sequence-specific mediators of RNA interference
US8420391B2 (en) 2000-03-30 2013-04-16 University Of Massachusetts RNA sequence-specific mediators of RNA interference
US7662951B2 (en) 2000-08-30 2010-02-16 Sirna Therapeutics, Inc. RNA interference mediated treatment of Alzheimer's disease using short interfering nucleic acid (siNA)
US8017765B2 (en) 2000-08-30 2011-09-13 Merck Sharp & Dohme Corp. RNA interference mediated treatment of alzheimer's disease using short interfering nucleic acid (siNA)
WO2002059257A3 (en) * 2000-10-31 2002-12-19 Commw Scient Ind Res Org Method and means for producing barley yellow dwarf virus resistant cereal plants
EP3199631A1 (en) * 2000-12-01 2017-08-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Rna interference mediating small rna molecules
US8372968B2 (en) 2000-12-01 2013-02-12 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8445237B2 (en) 2000-12-01 2013-05-21 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
JP2009284915A (ja) * 2000-12-01 2009-12-10 Max-Planck-Ges Zur Foerderung Der Wissenschaften Ev Ｒｎａ干渉を媒介する短鎖ｒｎａ分子
US8853384B2 (en) 2000-12-01 2014-10-07 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
EP2351852B2 (en) † 2000-12-01 2020-07-01 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8895718B2 (en) 2000-12-01 2014-11-25 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8895721B2 (en) 2000-12-01 2014-11-25 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
EP2813582A1 (en) * 2000-12-01 2014-12-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8796016B2 (en) 2000-12-01 2014-08-05 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8933044B2 (en) 2000-12-01 2015-01-13 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
KR100909681B1 (ko) * 2000-12-01 2009-07-29 막스-플랑크-게젤샤프트 츄어 푀르더룽 데어 비쎈샤프텐 에.파우. Ｒｎａ 간섭을 매개하는 작은 ｒｎａ 분자
US8778902B2 (en) 2000-12-01 2014-07-15 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US7056704B2 (en) 2000-12-01 2006-06-06 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US8993745B2 (en) 2000-12-01 2015-03-31 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US8765930B2 (en) 2000-12-01 2014-07-01 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
EP2351852B1 (en) * 2000-12-01 2013-10-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US10633656B2 (en) 2000-12-01 2020-04-28 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. RNA interference mediating small RNA molecules
US8329463B2 (en) 2000-12-01 2012-12-11 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. RNA interference mediating small RNA molecules
US7078196B2 (en) 2000-12-01 2006-07-18 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften, E.V. RNA interference mediating small RNA molecules
KR100872437B1 (ko) 2000-12-01 2008-12-05 막스-플랑크-게젤샤프트 츄어 푀르더룽 데어 비쎈샤프텐 에.파우. Ｒｎａ 간섭을 매개하는 작은 ｒｎａ 분자
CZ308053B6 (cs) * 2000-12-01 2019-11-27 Max Planck Gesellschaft Izolovaná molekula dvouřetězcové RNA, způsob její výroby a její použití
EP1407044B2 (en) † 2000-12-01 2017-11-15 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Rna interference mediating small rna molecules
US8362231B2 (en) 2000-12-01 2013-01-29 Max-Planck-Gesellschaft zur Föderung der Wissenschaften E.V. RNA interference mediating small RNA molecules
EP2213736A3 (de) * 2001-01-09 2010-09-01 Alnylam Europe AG Verfahren zur Hemmung der Expression eines Zielgens und Medikament zur Therapie einer Tumorerkrankung
US7868160B2 (en) 2001-01-09 2011-01-11 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
DE10100587C1 (de) * 2001-01-09 2002-11-21 Ribopharma Ag Verfahren zur Hemmung der Expression eines Zielgens
EP2365075A1 (de) 2001-01-09 2011-09-14 Alnylam Europe AG Verfahren zur Hemmung der Expression eines Zielgens und Medikament zur Therapie einer Tumorerkrankung
WO2002055692A3 (de) * 2001-01-09 2003-06-12 Ribopharma Ag Verfahren zur hemmung der expression eines zielgens und medikament zur therapie einer tumorerkrankung
US7767802B2 (en) 2001-01-09 2010-08-03 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
WO2002055693A3 (de) * 2001-01-09 2003-07-17 Ribopharma Ag Verfahren zur hemmung der expression eines zielgens
DE10100586C1 (de) * 2001-01-09 2002-04-11 Ribopharma Ag Verfahren zur Hemmung der Expression eines Ziegens
US7473525B2 (en) 2001-01-09 2009-01-06 Alnylam Europe Ag Compositions and methods for inhibiting expression of anti-apoptotic genes
US9074213B2 (en) 2001-01-09 2015-07-07 Alnylam Pharmacuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US9587240B2 (en) 2001-01-09 2017-03-07 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a target gene
US7423142B2 (en) 2001-01-09 2008-09-09 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of anti-apoptotic genes
WO2002068635A3 (en) * 2001-02-28 2003-12-04 Novartis Forschungsstiftung Methods of inhibiting expression of a target gene in mammalian cells
US7517864B2 (en) 2001-05-18 2009-04-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US20150105445A1 (en) * 2001-05-18 2015-04-16 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING CHEMICALLY MODIFIED SHORT INTERFERING NUCLEIC ACID (siNA)
US7659389B2 (en) 2001-05-18 2010-02-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of MYC and/or MYB gene expression using short interfering nucleic acid (siNA)
US8188247B2 (en) 2001-05-18 2012-05-29 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
EP2415486A3 (en) * 2001-05-18 2013-01-09 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US9994853B2 (en) 2001-05-18 2018-06-12 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
WO2005019453A3 (en) * 2001-05-18 2005-06-23 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING CHEMICALLY MODIFIED SHORT INTERFERING NUCLEIC ACID (siNA)
US7833992B2 (en) 2001-05-18 2010-11-16 Merck Sharpe & Dohme Conjugates and compositions for cellular delivery
EP1572067A4 (en) * 2001-05-18 2009-05-13 Sirna Therapeutics Inc CONJUGATES AND COMPOSITIONS FOR CELLULAR RELEASE
EP3231445A1 (en) 2001-05-18 2017-10-18 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US7964578B2 (en) 2001-05-18 2011-06-21 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US7858625B2 (en) 2001-05-18 2010-12-28 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US8017761B2 (en) 2001-05-18 2011-09-13 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of Stearoyl-CoA desaturase (SCD) gene expression using short interfering nucelic acid (siNA)
EP2415486A2 (en) 2001-05-18 2012-02-08 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US7491805B2 (en) 2001-05-18 2009-02-17 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US8008472B2 (en) 2001-05-29 2011-08-30 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of human immunodeficiency virus (HIV) gene expression using short interfering nucleic acid (siNA)
US10731155B2 (en) 2001-07-12 2020-08-04 University Of Massachusetts In vivo production of small interfering RNAs that mediate gene silencing
US8557785B2 (en) 2001-07-12 2013-10-15 University Of Massachusetts In vivo production of small interfering RNAS that mediate gene silencing
US7691995B2 (en) 2001-07-12 2010-04-06 University Of Massachusetts In vivo production of small interfering RNAS that mediate gene silencing
US7893036B2 (en) 2001-07-12 2011-02-22 University Of Massachusetts In vivo production of small interfering RNAs that mediate gene silencing
US8530438B2 (en) 2001-07-12 2013-09-10 University Of Massachusetts Vivo production of small interfering RNAs that mediate gene silencing
US9175287B2 (en) 2001-07-12 2015-11-03 University Of Massachusetts In vivo production of small interfering RNAs that mediate gene silencing
US8232260B2 (en) 2001-07-12 2012-07-31 University Of Massachusetts In vivo production of small interfering RNAs that mediate gene silencing
US9850487B2 (en) 2001-07-12 2017-12-26 University Of Massachusetts In vivo production of small interfering RNAs that mediate gene silencing
US8022272B2 (en) 2001-07-13 2011-09-20 Sungene Gmbh & Co. Kgaa Expression cassettes for transgenic expression of nucleic acids
US8604278B2 (en) 2001-07-13 2013-12-10 Sungene Gmbh & Co. Kgaa Expression cassettes for transgenic expression of nucleic acids
WO2003008573A3 (en) * 2001-07-17 2003-07-17 Anne Josephine Milner Silencing of gene expression by sirna
US7456335B2 (en) 2001-09-03 2008-11-25 Basf Plant Science Gmbh Nucleic acid sequences and their use in methods for achieving pathogen resistance in plants
US7745418B2 (en) 2001-10-12 2010-06-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting viral replication
WO2003033700A1 (de) * 2001-10-12 2003-04-24 Ribopharma Ag Verfahren zur hemmung der replikation von viren
US7763590B2 (en) 2001-10-12 2010-07-27 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a mutant gene
US7348314B2 (en) 2001-10-12 2008-03-25 Alnylam Europe Ag Compositions and methods for inhibiting viral replication
WO2003035868A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Medikament zur erhöhung der wirksamkeit eines rezeptor-vermittelt apoptose in tumorzellen auslösenden arzeimittels
WO2003035082A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Medikament zur hemmung der expression eines zielgens
WO2003035876A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Verwendung einer doppelsträngigen ribonukleinsäure zur behandlung einer infektion mit einem (+)-strang-rna-virus
WO2003035869A1 (de) * 2001-10-26 2003-05-01 Ribopharma Ag Verwendung einer doppelsträngigen ribonukleinsäure zur gezielten hemmung der expression eines vorgegebenen zielgens
US8829264B2 (en) 2002-01-22 2014-09-09 Cold Spring Harbor Laboratory Methods and compositions for RNA interference
WO2003062423A3 (de) * 2002-01-22 2004-07-22 Ribopharma Ag Verfahren zur hemmung der expression eines durch eine chromosomen-aberration entstandenen zielgens
US7846907B2 (en) 2002-01-22 2010-12-07 Alnylam Pharmaceuticals, Inc. Double-stranded RNA (dsRNA) and method of use for inhibiting expression of a fusion gene
US10626398B2 (en) 2002-02-01 2020-04-21 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US10196640B1 (en) 2002-02-01 2019-02-05 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
EP3415625A1 (en) * 2002-02-01 2018-12-19 Life Technologies Corporation Double-stranded oligonucleotides
EP2455467A1 (en) * 2002-02-01 2012-05-23 Life Technologies Corporation Double-stranded oligonucleotides
EP2924116A1 (en) * 2002-02-01 2015-09-30 Life Technologies Corporation Double-stranded oligonucleotides
JP2006501808A (ja) * 2002-02-01 2006-01-19 セクイター インコーポレイテッド 増強された効力を有するオリゴヌクレオチド組成物
US9592250B2 (en) 2002-02-01 2017-03-14 Life Technologies Corporation Double-stranded oligonucleotides
EP1572902B1 (en) * 2002-02-01 2014-06-11 Life Technologies Corporation HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
US9796978B1 (en) 2002-02-01 2017-10-24 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
EP2213292A1 (en) * 2002-02-01 2010-08-04 Life Technologies Corporation Double-stranded oligonucleotides
US10106793B2 (en) 2002-02-01 2018-10-23 Life Technologies Corporation Double-stranded oligonucleotides
US10036025B2 (en) 2002-02-01 2018-07-31 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US9777275B2 (en) 2002-02-01 2017-10-03 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US9771588B2 (en) 2002-02-20 2017-09-26 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US8153778B2 (en) 2002-02-20 2012-04-10 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of vascular cell adhesion molecule (VCAM) gene expression using short interfering nucleic acid (siNA)
US7667029B2 (en) 2002-02-20 2010-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of checkpoint kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA)
US7667030B2 (en) 2002-02-20 2010-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of matrix metalloproteinase 13 (MMP13) gene expression using short interfering nucleic acid (siNA)
US8846894B2 (en) 2002-02-20 2014-09-30 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7678897B2 (en) 2002-02-20 2010-03-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of platelet-derived endothelial cell growth factor (ECGF1) gene expression using short interfering nucleic acid (siNA)
WO2003072705A3 (en) * 2002-02-20 2003-11-06 Sirna Therapeutics Inc Rna interference mediated inhibition of cyclin d1 gene expression using short interfering nucleic acid (sina)
US7943757B2 (en) 2002-02-20 2011-05-17 Mcswiggen James RNA interference mediated inhibition of intercellular adhesion molecule (ICAM) gene expression using short interfering nucleic acid (siNA)
US8258288B2 (en) 2002-02-20 2012-09-04 Sirna Therapeutics, Inc. RNA interference mediated inhibition of respiratory syncytial virus (RSV) expression using short interfering nucleic acid (siNA)
US7659390B2 (en) 2002-02-20 2010-02-09 Sirna Therapeutics, Inc. RNA interference mediated inhibition of muscarinic colinergic receptor gene expression using short interfering nucleic acid (siNA)
EP3354656A1 (en) 2002-02-20 2018-08-01 Sirna Therapeutics, Inc. Rna interference mediated inhibition of gene expression using short interfering nucleic acid (sina)
EP1430157B1 (en) * 2002-02-20 2011-08-10 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF HEPATITIS C VIRUS (HCV) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US7858771B2 (en) 2002-02-20 2010-12-28 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of muscarinic colinergic receptor gene expression using short interfering nucleic acid (siNA)
US8067575B2 (en) 2002-02-20 2011-11-29 Merck, Sharp & Dohme Corp. RNA interference mediated inhibition of cyclin D1 gene expression using short interfering nucleic acid (siNA)
US7985853B2 (en) 2002-02-20 2011-07-26 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of platelet derived growth factor (PDGF) and platelet derived growth factor receptor (PDGFR) gene expression using short interfering nucleic acid (siNA)
US7935812B2 (en) 2002-02-20 2011-05-03 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of hepatitis C virus (HCV) expression using short interfering nucleic acid (siNA)
US7928218B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of polycomb group protein EZH2 gene expression using short interfering nucleic acid (siNA)
US7855284B2 (en) 2002-02-20 2010-12-21 Sirna Therapeutics, Inc. RNA interference mediated inhibition of checkpoint kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA)
US7691999B2 (en) 2002-02-20 2010-04-06 Sirna Therapeutics, Inc. RNA interference mediated inhibition of NOGO and NOGO receptor gene expression using short interfering nucleic acid (siNA)
US7928220B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of stromal cell-derived factor-1 (SDF-1) gene expression using short interfering nucleic acid (siNA)
US7928219B2 (en) 2002-02-20 2011-04-19 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of placental growth factor gene expression using short interfering nucleic acid (SINA)
EP1472269A4 (en) * 2002-02-20 2005-03-23 Sirna Therapeutics Inc RNA INTERFERENCE-INDUCED INHIBITION OF EXPRESSION OF INTERLEUKIN GENE USING SHORT INTERFERENT NUCLEIC ACIDS (SINA)
US7700760B2 (en) 2002-02-20 2010-04-20 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular cell adhesion molecule (VCAM) gene expression using short interfering nucleic acid (siNA)
US9738899B2 (en) 2002-02-20 2017-08-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US10351852B2 (en) 2002-02-20 2019-07-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
EP2042510A3 (en) * 2002-02-20 2009-06-24 Sirna Therapeutics Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleid acid (siNA)
EP1423404B1 (en) * 2002-02-20 2011-08-24 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED TREATMENT OF ALZHEIMER'S DISEASE USING SHORT INTERFERING NUCLEIC ACID (siNA)
EP1572128B1 (en) * 2002-02-20 2011-08-24 Sirna Therapeutics, Inc. RNA INTERFERENCE MEDIATED INHIBITION OF HIV GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US9732344B2 (en) 2002-02-20 2017-08-15 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7923549B2 (en) 2002-02-20 2011-04-12 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
EP2042510A2 (en) 2002-02-20 2009-04-01 Sirna Therapeutics Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleid acid (siNA)
EP1521768A4 (en) * 2002-02-20 2005-07-06 Sirna Therapeutics Inc INHIBITION OF THE VASCULAR ENDOTHELIC GROWTH FACTOR AND THE EXPRESSION OF THE GENE TO THE RECEPTOR OF THE VASCULAR ENDOTHELIC GROWTH FACTOR USING SINA (SHORT INTERFERING NUCLEIC ACID) THROUGH RNA INTERFERENCE
US8273866B2 (en) 2002-02-20 2012-09-25 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SINA)
US7915400B2 (en) 2002-02-20 2011-03-29 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of hepatitis C virus (HCV) gene expression using short interfering nucleic acid (siNA)
US7956178B2 (en) 2002-02-20 2011-06-07 Mcswiggen James RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acid (siNA)
US10889815B2 (en) 2002-02-20 2021-01-12 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US10662428B2 (en) 2002-02-20 2020-05-26 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
JP2008283975A (ja) * 2002-02-20 2008-11-27 Sirna Therapeutics Inc 短干渉核酸（ｓｉＮＡ）を用いるＣ型肝炎ウイルス（ＨＣＶ）遺伝子発現のＲＮＡ干渉媒介性阻害
US7910725B2 (en) 2002-02-20 2011-03-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (siNA)
US8008473B2 (en) 2002-02-20 2011-08-30 Mcswiggen James RNA interference mediated inhibition of TNF and TNF receptor gene expression using short interfering nucleic acid (siNA)
US9957517B2 (en) 2002-02-20 2018-05-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US8013146B2 (en) 2002-02-20 2011-09-06 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of matrix metalloproteinase 13 (MMP13) gene expression using short interfering nucleic acid (siNA)
US8013143B2 (en) 2002-02-20 2011-09-06 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of CXCR4 gene expression using short interfering nucleic acid (siNA)
EP1442143A4 (en) * 2002-02-20 2005-02-16 Sirna Therapeutics Inc INHIBITION OF BCL2 GENE EXPRESSION MEDIATED BY RNA INTERFERENCE USING siNA (SHORT INTERFERING NUCLEIC ACID)
EP1741781A3 (en) * 2002-02-20 2007-06-06 Sirna Therapeutics, Inc. RNA interference mediated treatment of Alzheimer's disease using short interfering nucleic acid (siNA)
EP1710307A3 (en) * 2002-02-20 2007-06-06 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
US7176304B2 (en) 2002-02-20 2007-02-13 Mcswiggen James RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US7977472B2 (en) 2002-02-20 2011-07-12 Leonid Beigelman RNA interference mediated inhibition of myostatin gene expression using short interfering nucleic acid (siNA)
EP1476457A4 (en) * 2002-02-20 2005-08-31 Ribozyme Pharm Inc Inhibition of the expression of the protein-tyrosine phosphatase-1B gene (PTP-1B) mediated by RNA interference, using SiNA (short interferon nucleotide acide)
US7910724B2 (en) 2002-02-20 2011-03-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Fos gene expression using short interfering nucleic acid (siNA)
EP1423406B1 (en) * 2002-02-20 2010-09-01 Sirna Therapeutics, Inc. Rna interference mediated inhibition of gene expression using short interfering nucleic acid (sina)
EP2902406A1 (en) 2002-02-20 2015-08-05 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
US7897756B2 (en) 2002-02-20 2011-03-01 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of NOGO and NOGO receptor gene expression using short interfering nucleic acid (siNA)
US7795422B2 (en) 2002-02-20 2010-09-14 Sirna Therapeutics, Inc. RNA interference mediated inhibition of hypoxia inducible factor 1 (HIF1) gene expression using short interfering nucleic acid (siNA)
US7897755B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of platelet-derived endothelial cell growth factor (ECGF1) gene expression using short interfering nucleic acid (siNA)
US9657294B2 (en) 2002-02-20 2017-05-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7897752B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of telomerase gene expression using short interfering nucleic acid (siNA)
US7897753B2 (en) 2002-02-20 2011-03-01 Sirna Therapeutics, Inc. RNA interference mediated inhibition of XIAP gene expression using short interfering nucleic acid (siNA)
US7897757B2 (en) 2002-02-20 2011-03-01 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of protein tyrosine phosphatase-1B (PTP-1B) gene expression using short interfering nucleic acid (siNA)
US9181551B2 (en) 2002-02-20 2015-11-10 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
EP1432724A4 (en) * 2002-02-20 2006-02-01 Sirna Therapeutics Inc INTERFERENCE MEDIATION INHIBITION OF GENE RNA FROM MAP KINASE
EP2287305A1 (en) 2002-02-20 2011-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
EP3926046A2 (en) 2002-02-20 2021-12-22 Sirna Therapeutics, Inc. Rna interference mediated inhibition of gene expression using short interfering nucleic acid (sina)
EP2287306A1 (en) 2002-02-20 2011-02-23 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
EP1478730A4 (en) * 2002-02-20 2006-01-25 Sirna Therapeutics Inc INTERFERENCE RNA-INDUCED INHIBITION OF THE GENE EXPRESSION OF SUPERFAMILY TFN AND TFN RECEPTOR SUPERFAMILY USING SHORT INTERFERENCE NUCLEIC ACID (SINA)
EP1470256A4 (en) * 2002-02-20 2006-01-25 Sirna Therapeutics Inc RNA INTERFERENCE-FACILITATED INHIBITION OF GENE EXPRESSION OF GASTRIC INHIBITING POLYPEPTIDES (GIP) USING SHORT INTERFERENCE NUCLEIC ACID (SINA)
EP1499631A4 (en) * 2002-02-20 2006-01-25 Sirna Therapeutics Inc RNA INTERFERENCE-INDUCED INHIBITION OF GENE EXPRESSION OF TGF-BETA RECEPTOR AND TGF-BETA USING SMALL INTERFERING NUCLEIC ACID (SINA)
US7662952B2 (en) 2002-02-20 2010-02-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acid (siNA)
EP3459963A1 (en) 2002-02-20 2019-03-27 Sirna Therapeutics, Inc. Rna interference mediated inhibition of gene expression using short interfering nucleic acid (sina)
US7893248B2 (en) 2002-02-20 2011-02-22 Sirna Therapeutics, Inc. RNA interference mediated inhibition of Myc and/or Myb gene expression using short interfering nucleic acid (siNA)
US10000754B2 (en) 2002-02-20 2018-06-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US8076472B2 (en) 2002-02-20 2011-12-13 Merck Sharp & Dohme Corp. RNA interference mediated inhibition of muscarinic colinergic receptor gene expression using short interfering nucleic acid (siNA)
EP1501853A4 (en) * 2002-02-20 2005-11-16 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION OF AN EPIDERMAL GROWTH FACTOR RECEPTOR USING SHORT INTERFERING NUCLEIC ACIDS
EP1472267A4 (en) * 2002-02-20 2005-11-09 Sirna Therapeutics Inc INHIBITION OF INDUCED HEPATITIS C VIRUS (HCV) GENE EXPRESSION BY INTERFERENCE OF RNA USING SHORT INTERFERENCE NUCLEIC ACID
EP2278004A1 (en) 2002-02-20 2011-01-26 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA)
EP1458741A4 (en) * 2002-02-20 2005-09-14 Sirna Therpeutics Inc RNA-Interferenz-mediated inhibition of genepression using chemically modified short-chain nucleic acid
US10280430B2 (en) 2002-03-21 2019-05-07 Monsanto Technology Llc Nucleic acid constructs and methods for producing altered seed oil compositions
US7566813B2 (en) 2002-03-21 2009-07-28 Monsanto Technology, L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
US8802922B2 (en) 2002-03-21 2014-08-12 Monsanto Technology Llc Nucleic acid constructs and methods for producing altered seed oil compositions
US7601888B2 (en) 2002-03-21 2009-10-13 Monsanto Technology L.L.C. Nucleic acid constructs and methods for producing altered seed oil compositions
US8202845B2 (en) 2002-04-18 2012-06-19 Acuity Pharmaceuticals, Inc. Means and methods for the specific modulation of target genes in the CNS and the eye and methods for their identification
US8946180B2 (en) 2002-04-18 2015-02-03 Opko Pharmaceuticals, Llc Means and methods for the specific modulation of target genes in the CNS and the eye and methods for their identification
US8299042B2 (en) 2002-04-26 2012-10-30 Alnylam Pharmaceuticals, Inc. Methods and compositions for silencing genes without inducing toxicity
US7399586B2 (en) 2002-05-23 2008-07-15 Ceptyr, Inc. Modulation of biological signal transduction by RNA interference
US7166771B2 (en) 2002-06-21 2007-01-23 Monsanto Technology Llc Coordinated decrease and increase of gene expression of more than one gene using transgenic constructs
US7893249B2 (en) 2002-07-12 2011-02-22 Sirna Therapeutics, Inc. Deprotection and purification of oligonucleotides and their derivatives
US7655790B2 (en) 2002-07-12 2010-02-02 Sirna Therapeutics, Inc. Deprotection and purification of oligonucleotides and their derivatives
US9150863B2 (en) 2002-07-24 2015-10-06 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiogenesis
US8946403B2 (en) 2002-07-24 2015-02-03 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiogenesis
US8546345B2 (en) 2002-07-24 2013-10-01 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiogenesis
US8541384B2 (en) 2002-07-24 2013-09-24 The Trustees Of The University Of Pennsylvania Compositions and methods for siRNA inhibition of angiogenesis
US7732658B2 (en) 2002-07-25 2010-06-08 Dana Farber Cancer Institute, Inc. Composition and method for imaging cells
EP2036984A2 (de) 2002-07-26 2009-03-18 BASF Plant Science GmbH Revertierung der negativ-selektiven Wirkung von negativen Markerproteinen als Selektionsverfahren
US9695423B2 (en) 2002-08-05 2017-07-04 Silence Therapeutics Gmbh Interfering RNA molecules
US9790505B2 (en) 2002-08-05 2017-10-17 Silence Therapeutics Gmbh Interfering RNA molecules
US7893245B2 (en) 2002-08-05 2011-02-22 Silence Therapeutics Aktiengesellschaft (Ag) Interfering RNA molecules
US9222092B2 (en) 2002-08-05 2015-12-29 Silence Therapeutics Gmbh Interfering RNA molecules
WO2004015107A3 (en) * 2002-08-05 2004-04-29 Atugen Ag Further novel forms of interfering rna molecules
EP1857547B1 (en) 2002-08-05 2018-01-17 Silence Therapeutics GmbH Further novel forms of interfering RNA molecules
US10072264B2 (en) 2002-08-05 2018-09-11 University Of Iowa Research Foundation RNA interference suppression of neurodegenerative diseases and methods of use
US8481710B2 (en) 2002-08-05 2013-07-09 University Of Iowa Research Foundation RNA interference suppression of neurodegenerative diseases and methods of use thereof
US10266829B2 (en) 2002-08-05 2019-04-23 Silence Therapeutics Gmbh Interfering RNA molecules
US11578328B2 (en) 2002-08-05 2023-02-14 Silence Therapeutics Gmbh Interfering RNA molecules
US8524879B2 (en) 2002-08-05 2013-09-03 University Of Iowa Research Foundation RNA interference suppresion of neurodegenerative diseases and methods of use thereof
US10774332B2 (en) 2002-08-05 2020-09-15 Silence Therapeutics Gmbh Interfering RNA molecules
US9260716B2 (en) 2002-08-05 2016-02-16 University Of Iowa Research Foundation RNA interference suppression of neurodegenerative diseases and methods of use thereof
EP3222725A1 (en) 2002-08-05 2017-09-27 Silence Therapeutics GmbH Further novel forms of interfering rna molecules
US8329890B2 (en) 2002-08-05 2012-12-11 University Of Iowa Research Foundation SiRNA-mediated gene silencing
US9487779B2 (en) 2002-08-05 2016-11-08 University Of Iowa Research Foundation siRNA-mediated gene silencing
US8324370B2 (en) 2002-08-05 2012-12-04 Silence Therapeutics Aktiengesellschaft (Ag) Interfering RNA molecules
US7452987B2 (en) 2002-08-05 2008-11-18 Silence Therapeutics Aktiengesellschaft (Ag) Interfering RNA molecules
KR101201664B1 (ko) 2002-08-05 2012-11-15 사일런스 테라퓨틱스 아게 신규한 형태의 간섭 ｒｎａ 분자
EP2258847B1 (en) 2002-08-05 2017-03-15 Silence Therapeutics GmbH Futher novel forms of interfering RNA molecules
EP1857547A3 (en) * 2002-08-05 2011-05-18 Silence Therapeutics Aktiengesellschaft Further novel forms of interfering RNA molecules
US9790501B2 (en) 2002-08-05 2017-10-17 Silence Therapeutics Gmbh Interfering RNA molecules
US9783802B2 (en) 2002-08-05 2017-10-10 Silence Therapeutics Gmbh Interfering RNA molecules
EP1389637A1 (en) * 2002-08-05 2004-02-18 atugen AG Novel forms of interfering RNA molecules
US10329568B2 (en) 2002-08-05 2019-06-25 Silence Therapeutics Gmbh Interfering RNA molecules
EP2258847A3 (en) * 2002-08-05 2011-05-18 Silence Therapeutics AG Futher novel forms of interfering RNA molecules
US9758784B1 (en) 2002-08-05 2017-09-12 Silence Therapeutics Gmbh Interfering RNA molecules
US8779116B2 (en) 2002-08-05 2014-07-15 University Of Iowa Research Foundation SiRNA-mediated gene silencing
EP2258847A2 (en) 2002-08-05 2010-12-08 Silence Therapeutics AG Futher novel forms of interfering RNA molecules
US8933215B2 (en) 2002-08-05 2015-01-13 Silence Therapeutics Aktiengesellschaft (Ag) Interfering RNA molecules
US10323246B2 (en) 2002-08-05 2019-06-18 Silence Therapeutics Gmbh Interfering RNA molecules
EP3222726A1 (en) 2002-08-05 2017-09-27 Silence Therapeutics GmbH Further novel forms of interfering rna molecules
EP3222724A1 (en) 2002-08-05 2017-09-27 Silence Therapeutics GmbH Further novel forms of interfering rna molecules
US8729036B2 (en) 2002-08-07 2014-05-20 University Of Massachusetts Compositions for RNA interference and methods of use thereof
EP2278018A2 (en) 2002-08-07 2011-01-26 BASF Plant Science GmbH Nucleic acid sequences encoding proteins associated with abiotic stress response
WO2004018687A2 (en) 2002-08-07 2004-03-04 Basf Plant Science Gmbh Nucleic acid sequences encoding proteins associated with abiotic stress response
US9611472B2 (en) 2002-08-07 2017-04-04 University Of Massachusetts Compositions for RNA interference and methods of use thereof
US7713943B2 (en) 2002-08-14 2010-05-11 Silence Therapeutics Aktiengesellschaft (Ag) Further use of protein kinase N beta
US9078855B2 (en) 2002-08-14 2015-07-14 Silence Therapeutics Gmbh Further use of protein kinase N beta
EP2055310A1 (en) 2002-08-14 2009-05-06 Silence Therapeutics Aktiengesellschaft Further use of protein kinase N beta
EP3040081A2 (en) 2002-08-14 2016-07-06 Silence Therapeutics GmbH Use of protein kinase n beta
WO2004022075A1 (en) 2002-09-04 2004-03-18 Novartis Ag Treatment of neurological disorders by dsrna adminitration
US7923547B2 (en) 2002-09-05 2011-04-12 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US7407792B2 (en) 2002-10-10 2008-08-05 Wyeth Compositions, organisms and methodologies employing a novel human kinase
US7122361B2 (en) 2002-10-10 2006-10-17 Wyeth Compositions employing a novel human kinase
EP2072619A1 (en) 2002-10-18 2009-06-24 Silence Therapeutics Aktiengesellschaft Factor involved in metastasis and uses thereof
US7208306B2 (en) 2002-10-24 2007-04-24 Wyeth Compositions employing a novel human protein phosphatase
WO2004044138A2 (en) 2002-11-05 2004-05-27 Isis Pharmaceuticals, Inc. Chimeric oligomeric compounds and their use in gene modulation
US9150606B2 (en) 2002-11-05 2015-10-06 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2'-modified nucleosides for use in gene modulation
US9150605B2 (en) 2002-11-05 2015-10-06 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2′-modified nucleosides for use in gene modulation
US8124745B2 (en) 2002-11-05 2012-02-28 Isis Pharmaceuticals, Inc Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
WO2004043979A2 (en) 2002-11-05 2004-05-27 Isis Pharmaceuticals, Inc. Sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
US7696345B2 (en) 2002-11-05 2010-04-13 Isis Pharmaceuticals, Inc. Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
WO2004044136A2 (en) 2002-11-05 2004-05-27 Isis Pharmaceuticals, Inc. Compositions comprising alternating 2’-modified nucleosides for use in gene modulation
JP2006505280A (ja) * 2002-11-07 2006-02-16 チャン・ルン−ジ 改変樹状細胞
US9839649B2 (en) 2002-11-14 2017-12-12 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US9719094B2 (en) 2002-11-14 2017-08-01 Thermo Fisher Scientific Inc. RNAi targeting SEC61G
US10011836B2 (en) 2002-11-14 2018-07-03 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US7807820B2 (en) 2002-11-14 2010-10-05 Dharmacon, Inc. siRNA targeting beta secretase (BACE)
US9771586B2 (en) 2002-11-14 2017-09-26 Thermo Fisher Scientific Inc. RNAi targeting ZNF205
US11198870B2 (en) 2002-11-14 2021-12-14 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US9879266B2 (en) 2002-11-14 2018-01-30 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US10233449B2 (en) 2002-11-14 2019-03-19 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US10765695B2 (en) 2002-11-14 2020-09-08 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US9777270B2 (en) 2002-11-14 2017-10-03 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US10696968B2 (en) 2002-11-14 2020-06-30 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US9719092B2 (en) 2002-11-14 2017-08-01 Thermo Fisher Scientific Inc. RNAi targeting CNTD2
US9228186B2 (en) 2002-11-14 2016-01-05 Thermo Fisher Scientific Inc. Methods and compositions for selecting siRNA of improved functionality
US7611839B2 (en) 2002-11-21 2009-11-03 Wyeth Methods for diagnosing RCC and other solid tumors
JP2004173512A (ja) * 2002-11-22 2004-06-24 Takashi Morita ＲＮＡｉによる新規治療法および治療剤
US7297525B2 (en) 2002-11-27 2007-11-20 Wyeth Composition employing a novel human kinase
EP2058408A2 (en) 2003-02-14 2009-05-13 Sagres Discovery, Inc. Therapeutic GPCR targets in cancer
US11530408B2 (en) 2003-03-07 2022-12-20 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
US8445665B2 (en) 2003-03-07 2013-05-21 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
US10669544B2 (en) 2003-03-07 2020-06-02 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
US8754201B2 (en) 2003-03-07 2014-06-17 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
US9708615B2 (en) 2003-03-07 2017-07-18 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
US8420799B2 (en) 2003-03-07 2013-04-16 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
US9222091B2 (en) 2003-03-07 2015-12-29 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
EP3450559A1 (en) 2003-03-07 2019-03-06 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
EP2216407A2 (en) 2003-03-07 2010-08-11 Alnylam Pharmaceuticals, Inc. Therapeutic Compositions
US10273477B2 (en) 2003-03-07 2019-04-30 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
EP2239329A1 (en) 2003-03-07 2010-10-13 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
US8809516B2 (en) 2003-03-07 2014-08-19 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
US8110674B2 (en) 2003-03-07 2012-02-07 Alnylam Pharmaceuticals, Inc. Therapeutic compositions
US8653252B2 (en) * 2003-03-21 2014-02-18 Santaris Pharma A/S Short interfering RNA (siRNA) analogues
US9297010B2 (en) * 2003-03-21 2016-03-29 Roche Innovation Center Copenhagen A/S Short interfering RNA (siRNA) analogues
US9738894B2 (en) 2003-03-21 2017-08-22 Roche Innovation Center Copenhagen A/S Short interfering RNA (siRNA) analogues
US20070191294A1 (en) * 2003-03-21 2007-08-16 Santaris Pharma A/S Short interfering rna (sirna) analogues
US20140235844A1 (en) * 2003-03-21 2014-08-21 Santaris Pharma A/S Short Interfering RNA (siRNA) Analogues
WO2004091515A2 (en) 2003-04-09 2004-10-28 Alnylam Pharmaceuticals, Inc. iRNA CONJUGATES
WO2004092383A3 (en) * 2003-04-15 2005-02-10 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF SEVERE ACUTE RESPIRATORY SYNDROME (SARS) VIRUS GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
EP2669377A2 (en) 2003-04-17 2013-12-04 Alnylam Pharmaceuticals Inc. Modified iRNA agents
US11015194B2 (en) 2003-04-17 2021-05-25 Alnylam Pharmaceuticals, Inc. iRNA agents with biocleavable tethers
US8796436B2 (en) 2003-04-17 2014-08-05 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
US10676740B2 (en) 2003-04-17 2020-06-09 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
US8865677B2 (en) 2003-04-17 2014-10-21 Alnylam Pharmaceuticals, Inc. iRNA agents with biocleavable tethers
US9394540B2 (en) 2003-04-17 2016-07-19 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
US10119138B2 (en) 2003-04-17 2018-11-06 Alnylam Pharmaceuticals, Inc. iRNA agents with biocleavable tethers
US7745608B2 (en) 2003-04-17 2010-06-29 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
EP2666858A1 (en) 2003-04-17 2013-11-27 Alnylam Pharmaceuticals Inc. Modified iRNA agents
EP2664672A1 (en) 2003-04-17 2013-11-20 Alnylam Pharmaceuticals Inc. Modified iRNA agents
EP2660322A2 (en) 2003-04-17 2013-11-06 Alnylam Pharmaceuticals Inc. Modified iRNA agents
US8017762B2 (en) 2003-04-17 2011-09-13 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
US8507661B2 (en) 2003-04-17 2013-08-13 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
US11312957B2 (en) 2003-04-17 2022-04-26 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
US8426377B2 (en) 2003-04-17 2013-04-23 Alnylam Pharmaceuticals, Inc. iRNA agents with biocleavable tethers
US8344125B2 (en) 2003-04-17 2013-01-01 Alnylam Pharmaceuticals, Inc. Modified iRNA agents
WO2004094345A2 (en) 2003-04-17 2004-11-04 Alnylam Pharmaceuticals Inc. Protected monomers
US7851615B2 (en) 2003-04-17 2010-12-14 Alnylam Pharmaceuticals, Inc. Lipophilic conjugated iRNA agents
US9476045B2 (en) 2003-04-17 2016-10-25 Alnylam Pharmaceuticals, Inc. iRNA agents with biocleavable tethers
WO2004097020A3 (en) * 2003-04-25 2006-01-05 Sirna Therapeutics Inc Rna interference mediated inhibition of map kinase gene expression
EP3222294A1 (en) 2003-04-30 2017-09-27 Sirna Therapeutics, Inc. Conjugates and compositions for cellular delivery
US7595306B2 (en) 2003-06-09 2009-09-29 Alnylam Pharmaceuticals Inc Method of treating neurodegenerative disease
WO2005014782A2 (en) 2003-06-13 2005-02-17 Alnylam Europe Ag., Double-stranded ribonucleic acid with increased effectiveness in an organism
EP3604537A1 (en) 2003-06-13 2020-02-05 Alnylam Europe AG Double-stranded ribonucleic acid with increased effectiveness in an organism
EP2336317A1 (en) 2003-06-13 2011-06-22 Alnylam Europe AG Double-stranded ribonucleic acid with increased effectiveness in an organism
WO2005003350A3 (en) * 2003-06-27 2005-08-04 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED TREATMENT OF ALZHEIMER’S DISEASE USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2005007859A3 (en) * 2003-07-11 2005-12-01 Sirna Therapeutics Inc Rna interference mediated inhibition of acetyl-coa carboxylase gene expression using short interfering nucleic acid (sina)
EP2434019A1 (en) 2003-08-01 2012-03-28 BASF Plant Science GmbH Process for the production of fine chemicals
WO2005014811A3 (en) * 2003-08-08 2005-12-22 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF XIAP GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US7795504B2 (en) 2003-09-24 2010-09-14 Monsanto Technology Llc Coordinated decrease and increase of gene expression of more than one gene using transgenic constructs
WO2005045037A3 (en) * 2003-10-23 2005-11-03 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED INHIBITION OF 5-ALPHA REDUCTASE AND ANDROGEN RECEPTOR GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US7700280B2 (en) 2003-12-31 2010-04-20 The Penn State Research Foundation Methods for assessing cisplatin resistance, disease progression, and treatment efficacy in ovarian cancer
EP2395112A1 (en) 2003-12-31 2011-12-14 The Penn State Research Foundation Methods for predicting and overcoming resistance to chemotherapy in ovarian cancer and for predicting colon cancer occurrence
WO2005066371A2 (en) 2003-12-31 2005-07-21 The Penn State Research Foundation Methods for predicting and overcoming resistance to chemotherapy in ovarian cancer and for predicting colon cancer occurrence
US8192935B2 (en) 2003-12-31 2012-06-05 The Penn State Research Foundation Methods for assessing cisplatin resistance, disease progression, and treatment efficacy in ovarian cancer as related to MetAP2 expression
US7655402B2 (en) 2004-02-06 2010-02-02 Wyeth Llc Diagnoses and therapeutics for cancer
US7858769B2 (en) 2004-02-10 2010-12-28 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using multifunctional short interfering nucleic acid (multifunctional siNA)
US8476245B2 (en) 2004-02-11 2013-07-02 University Of Tennessee Research Foundation Inhibition of tumor growth and invasion by anti matrix metalloproteinase DNAzymes
US7781393B2 (en) 2004-02-25 2010-08-24 Dana-Farber Cancer Institute, Inc. Methods for inhibiting tumor cell growth
US7947659B2 (en) 2004-03-12 2011-05-24 Alnylam Pharmaceuticals, Inc. iRNA agents targeting VEGF
US7919473B2 (en) 2004-03-12 2011-04-05 Alnylam Pharmaceuticals, Inc. IRNA agents targeting VEGF
EP2394662A2 (en) 2004-04-02 2011-12-14 The Regents of The University of California Methods and compositions for treating and preventing disease associated with AlphavBeta5 intergrin
US8946402B2 (en) 2004-04-23 2015-02-03 The Trustees Of Columbia University In The City Of New York Inhibition of hairless protein mRNA
US8329667B2 (en) 2004-04-23 2012-12-11 The Trustees Of Columbia University In The City Of New York Inhibition of hairless protein mRNA
WO2005118787A2 (en) 2004-05-10 2005-12-15 The University Of North Carolina At Chapel Hill Method of screening candidate compounds for susceptibility to biliary excretion
US10508277B2 (en) 2004-05-24 2019-12-17 Sirna Therapeutics, Inc. Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
US10047388B2 (en) 2004-05-28 2018-08-14 Asuragen, Inc. Methods and compositions involving MicroRNA
US8394947B2 (en) 2004-06-03 2013-03-12 Isis Pharmaceuticals, Inc. Positionally modified siRNA constructs
US8088359B2 (en) 2004-06-04 2012-01-03 University Of Geneva Means and methods of using a NADPH oxidase inhibitor for the treatment of hearing loss and phantom hearing
US9650635B2 (en) 2004-06-04 2017-05-16 University Of Geneva Pharmaceutical compositions comprising small interfering RNA inhibitors of NOX3
US8722639B2 (en) 2004-06-04 2014-05-13 University Of Geneva Means and methods for the treatment of hearing loss and phantom hearing
EP1612266A2 (en) 2004-06-10 2006-01-04 National Taiwan University Prostaglandin reductase
WO2006009575A1 (en) 2004-06-22 2006-01-26 The Board Of Trustees Of The University Of Illinois METHODS OF INHIBITING TUMOR CELL PROLIFERATION WITH FOXM1 siRNA
EP2298896A1 (en) 2004-06-22 2011-03-23 The Board of Trustees of the University of Illinois Methods of inhibiting tumor cell proliferation with FOXM1 siRNA
EP2080769A2 (en) 2004-07-02 2009-07-22 Metanomics GmbH Process for the production of fine chemicals
WO2006069610A2 (en) 2004-07-02 2006-07-06 Metanomics Gmbh Process for the production of fine chemicals
US7968762B2 (en) 2004-07-13 2011-06-28 Van Andel Research Institute Immune-compromised transgenic mice expressing human hepatocyte growth factor (hHGF)
EP2204376A2 (en) 2004-07-20 2010-07-07 Sagres Discovery, Inc. Novel therapeutic targets in cancer
EP2484780A1 (en) 2004-07-23 2012-08-08 The University of North Carolina At Chapel Hill Methods and materials for determining pain sensibility and predicting and treating related disorders
EP2166103A1 (en) 2004-08-02 2010-03-24 BASF Plant Science GmbH Method for isolation of transcription termination sequences
EP2163635A1 (en) 2004-08-02 2010-03-17 BASF Plant Science GmbH Method for isolation of transcription termination sequences
EP2166104A1 (en) 2004-08-02 2010-03-24 BASF Plant Science GmbH Method for isolation of transcription termination sequences
WO2006017673A2 (en) 2004-08-03 2006-02-16 Biogen Idec Ma Inc. Taj in neuronal function
US8642571B2 (en) 2004-08-06 2014-02-04 Quark Pharmaceuticals, Inc. Therapeutic uses of inhibitors of RTP801
US8168607B2 (en) 2004-08-06 2012-05-01 Quark Pharmaceuticals Inc. Methods of treating eye diseases in diabetic patients
EP2990410A1 (en) 2004-08-10 2016-03-02 Alnylam Pharmaceuticals Inc. Chemically modified oligonucleotides
WO2006020768A2 (en) 2004-08-10 2006-02-23 Alnylam Pharmaceuticals, Inc. Chemically modified oligonucleotides
US7741299B2 (en) 2004-08-16 2010-06-22 Quark Pharmaceuticals, Inc. Therapeutic uses of inhibitors of RTP801
US8309532B2 (en) 2004-08-16 2012-11-13 Quark Pharmaceuticals, Inc. Therapeutic uses of inhibitors of RTP801
WO2006017932A1 (en) 2004-08-18 2006-02-23 Genesense Technologies Inc. Small interfering rna molecules against ribonucleotide reductase and uses thereof
US7884086B2 (en) 2004-09-08 2011-02-08 Isis Pharmaceuticals, Inc. Conjugates for use in hepatocyte free uptake assays
EP2382997A1 (en) 2004-09-15 2011-11-02 Alnylam Pharmaceuticals Compositions and methods for inhibiting expression of anti-apoptotic genes
WO2006032707A2 (en) 2004-09-24 2006-03-30 Basf Plant Science Gmbh Plant cells and plants with increased tolerance to environmental stress
US7842674B2 (en) 2004-09-28 2010-11-30 Quark Pharmaceuticals Inc. Methods of treatment of acute renal failure
US8765699B2 (en) 2004-09-28 2014-07-01 Quark Pharmaceuticals, Inc. Oligoribonucleotides and methods of use thereof for treatment of alopecia, acute renal failure and other diseases
US8148342B2 (en) 2004-09-28 2012-04-03 Quark Pharmaceuticals Inc. Oligoribonucleotides and methods of use thereof for treatment of alopecia, acute renal failure and other diseases
EP1645633A2 (en) 2004-10-05 2006-04-12 SunGene GmbH Constitutive expression cassettes for regulation of plant expression
EP2166097A1 (en) 2004-10-05 2010-03-24 SunGene GmbH Constitutive expression cassettes for regulation of plant expression
EP2166098A1 (en) 2004-10-05 2010-03-24 SunGene GmbH Constitutive expression cassettes for regulation of plant expression
US7915479B2 (en) 2004-10-13 2011-03-29 University Of Georgia Research Foundation, Inc. Nematode resistant transgenic plants
US9492400B2 (en) 2004-11-04 2016-11-15 Massachusetts Institute Of Technology Coated controlled release polymer particles as efficient oral delivery vehicles for biopharmaceuticals
EP1655364A2 (en) 2004-11-05 2006-05-10 BASF Plant Science GmbH Expression cassettes for seed-preferential expression in plants
US9506061B2 (en) 2004-11-12 2016-11-29 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US9447414B2 (en) 2004-11-12 2016-09-20 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US8946177B2 (en) 2004-11-12 2015-02-03 Mima Therapeutics, Inc Methods and compositions involving miRNA and miRNA inhibitor molecules
EP2163631A1 (en) 2004-11-25 2010-03-17 SunGene GmbH Expression cassettes for guard cell-preferential expression in plants
EP1662000A2 (en) 2004-11-25 2006-05-31 SunGene GmbH Expression cassettes for guard cell-preferential expression in plants
EP1666599A2 (en) 2004-12-04 2006-06-07 SunGene GmbH Expression cassettes for mesophyll- and/or epidermis-preferential expression in plants
EP1669455A2 (en) 2004-12-08 2006-06-14 SunGene GmbH Expression cassettes for vascular tissue-preferential expression in plants
EP2072620A2 (en) 2004-12-08 2009-06-24 SunGene GmbH Expression casstettes for vascular tissue-preferential expression in plants
EP1669456A2 (en) 2004-12-11 2006-06-14 SunGene GmbH Expression cassettes for meristem-preferential expression in plants
WO2007087815A2 (en) 2004-12-17 2007-08-09 Metanomics Gmbh Process for the control of production of fine chemicals
EP2199304A1 (en) 2004-12-17 2010-06-23 Metanomics GmbH Process for the control of production of fine chemicals
EP2096177A2 (en) 2004-12-17 2009-09-02 Metanomics GmbH Process for the production of lutein
US7838507B2 (en) 2004-12-23 2010-11-23 Alcon, Inc. RNAi inhibition of CTGF for treatment of ocular disorders
US7622454B2 (en) 2004-12-23 2009-11-24 Alcon, Inc. RNAi inhibition of CTGF for treatment of ocular disorders
US9387262B2 (en) 2004-12-27 2016-07-12 Silence Therapeutics Gmbh Coated lipid complexes and their use
US7592324B2 (en) 2005-02-01 2009-09-22 Alcon, Inc. RNAi-mediated inhibition of ocular targets
EP2186903A2 (en) 2005-02-09 2010-05-19 BASF Plant Science GmbH Expression cassettes for regulation of expression in monocotyledonous plants
WO2006089950A2 (en) 2005-02-26 2006-08-31 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
EP2166099A2 (en) 2005-03-08 2010-03-24 BASF Plant Science GmbH Expression enhancing intron sequences
EP2166100A2 (en) 2005-03-08 2010-03-24 BASF Plant Science GmbH Expression enhancing intron sequences
EP2045327A2 (en) 2005-03-08 2009-04-08 BASF Plant Science GmbH Expression enhancing intron sequences
EP2169058A2 (en) 2005-03-08 2010-03-31 BASF Plant Science GmbH Expression enhancing intron sequences
EP2166101A2 (en) 2005-03-08 2010-03-24 BASF Plant Science GmbH Expression enhancing intron sequences
EP2166102A2 (en) 2005-03-08 2010-03-24 BASF Plant Science GmbH Expression enhancing intron sequences
US9550994B2 (en) 2005-03-11 2017-01-24 Arrowhead Pharmaceuticals, Inc. RNAI-mediated inhibition of frizzled related protein-1 for treatment of glaucoma
US9040494B2 (en) 2005-03-11 2015-05-26 Novartis Ag RNAi-mediated inhibition of frizzled related protein-1 for treatment of glaucoma
US8173617B2 (en) 2005-03-11 2012-05-08 Novartis Ag RNAi-mediated inhibition of frizzled related protein-1 for treatment of glaucoma
US7947660B2 (en) 2005-03-11 2011-05-24 Alcon, Inc. RNAi-mediated inhibition of frizzled related protein-1 for treatment of glaucoma
EP2360249A1 (en) 2005-03-31 2011-08-24 Calando Pharmaceuticals, Inc. Inhibitors of ribonucleotide reductase subunit 2 and uses thereof
EP2083088A2 (en) 2005-04-07 2009-07-29 Novartis Vaccines and Diagnostics, Inc. Cancer-related genes
EP2062591A1 (en) 2005-04-07 2009-05-27 Novartis Vaccines and Diagnostics, Inc. CACNA1E in cancer diagnosis detection and treatment
WO2006120197A2 (en) 2005-05-10 2006-11-16 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
US7417072B2 (en) 2005-05-12 2008-08-26 Wisconsin Alumni Research Foundation Blockade of Pin1 prevents cytokine production by activated immune cells
EP1723958A2 (fr) 2005-05-19 2006-11-22 L'oreal Vectorisation de dsRNA par des particules cationiques et utilisation topique
EP2159289A2 (en) 2005-06-23 2010-03-03 BASF Plant Science GmbH Improved methods for the production of stably transformed plants
US8703769B2 (en) 2005-07-15 2014-04-22 The University Of North Carolina At Chapel Hill Use of EGFR inhibitors to prevent or treat obesity
EP2980220A1 (en) 2005-09-20 2016-02-03 BASF Plant Science GmbH Improved methods controlling gene expression
US8093369B2 (en) 2005-10-11 2012-01-10 Ben Gurion University Of The Negev Research And Development Authority Ltd. Compositions for silencing the expression of VDAC1 and uses thereof
US7320965B2 (en) 2005-10-28 2008-01-22 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Huntingtin gene
EP2325315A1 (en) 2005-10-28 2011-05-25 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of huntingtin gene
US8314075B2 (en) 2005-10-28 2012-11-20 Alynylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of huntingtin gene
US7749978B2 (en) 2005-10-28 2010-07-06 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Huntingtin gene
US8188263B2 (en) 2005-11-02 2012-05-29 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US8513403B2 (en) 2005-11-02 2013-08-20 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US8101741B2 (en) 2005-11-02 2012-01-24 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US9074208B2 (en) 2005-11-02 2015-07-07 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US7582745B2 (en) 2005-11-04 2009-09-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Nav1.8 gene
WO2007054441A2 (de) 2005-11-08 2007-05-18 Basf Plant Science Gmbh Verwendung von armadillo-repeat (arm1)-polynukleotiden zum erreichen einer pathogenresistenz in pflanzen
EP2363134A1 (en) 2005-11-09 2011-09-07 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor V Leiden mutant gene
US9267937B2 (en) 2005-12-15 2016-02-23 Massachusetts Institute Of Technology System for screening particles
WO2007076904A1 (en) 2005-12-30 2007-07-12 Evonik Röhm Gmbh Peptides useful as cell-penetrating peptides
EP2330202A2 (en) 2006-01-06 2011-06-08 University Of Georgia Research Foundation, Inc. Cyst nematode resistant transgenic plants
EP2380986A1 (en) 2006-01-12 2011-10-26 BASF Plant Science GmbH Use of stomatin (STM1) polynucleotides for achieving a pathogen resistance in plants
US8067570B2 (en) 2006-01-20 2011-11-29 Quark Pharmaceuticals, Inc. Therapeutic uses of inhibitors of RTP801
US9056903B2 (en) 2006-01-20 2015-06-16 Quark Pharmaceuticals, Inc. Therapeutic uses of inhibitors of RTP801
US7825099B2 (en) 2006-01-20 2010-11-02 Quark Pharmaceuticals, Inc. Treatment or prevention of oto-pathologies by inhibition of pro-apoptotic genes
US8404654B2 (en) 2006-01-20 2013-03-26 Quark Pharmaceuticals, Inc. Treatment or prevention of oto-pathologies by inhibition of pro-apoptotic genes
EP2526968A2 (en) 2006-01-27 2012-11-28 Biogen Idec MA Inc. Nogo receptor antagonists
US11708577B2 (en) 2006-02-13 2023-07-25 Monsanto Technology Llc Modified gene silencing
US9765351B2 (en) 2006-02-13 2017-09-19 Monsanto Technology Llc Modified gene silencing
US7910566B2 (en) 2006-03-09 2011-03-22 Quark Pharmaceuticals Inc. Prevention and treatment of acute renal failure and other kidney diseases by inhibition of p53 by siRNA
EP2292739A1 (fr) 2006-03-24 2011-03-09 Institut National De La Recherche Agronomique Procédé de préparation de cellules aviaires differenciées et gènes impliqués dans le maintien de la pluripotence
US8968702B2 (en) 2006-03-30 2015-03-03 Duke University Inhibition of HIF-1 activation for anti-tumor and anti-inflammatory responses
WO2007115168A2 (en) 2006-03-31 2007-10-11 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of eg5 gene
US7718629B2 (en) 2006-03-31 2010-05-18 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Eg5 gene
EP2090662A2 (en) 2006-04-05 2009-08-19 Metanomics GmbH Process for the production of a fine chemical
EP2407483A1 (en) 2006-04-13 2012-01-18 Novartis Vaccines and Diagnostics, Inc. Methods of treating, diagnosing or detecting cancers
US7691824B2 (en) 2006-04-28 2010-04-06 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of a gene from the JC virus
EP3872179A1 (en) 2006-05-11 2021-09-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the pcsk9 gene
EP2584047A1 (en) 2006-05-11 2013-04-24 Alnylam Pharmaceuticals Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
EP2835429A1 (en) 2006-05-11 2015-02-11 Alnylam Pharmaceuticals Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
EP3578656A1 (en) 2006-05-11 2019-12-11 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the pcsk9 gene
EP2584048A1 (en) 2006-05-11 2013-04-24 Alnylam Pharmaceuticals Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
US9260718B2 (en) 2006-05-11 2016-02-16 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
US9822365B2 (en) 2006-05-11 2017-11-21 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
EP2194128A1 (en) 2006-05-11 2010-06-09 Alnylam Pharmaceuticals Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
US10501742B2 (en) 2006-05-11 2019-12-10 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
US7605251B2 (en) 2006-05-11 2009-10-20 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
EP3249052A1 (en) 2006-05-11 2017-11-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the pcsk9 gene
US8222222B2 (en) 2006-05-11 2012-07-17 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the PCSK9 gene
US8809292B2 (en) 2006-05-11 2014-08-19 Alnylam Pharmaceuticals, Inc Compositions and methods for inhibiting expression of the PCSK9 gene
US9688812B2 (en) 2006-05-15 2017-06-27 Massachusetts Institute Of Technology Polymers for functional particles
US9080014B2 (en) 2006-05-15 2015-07-14 Massachusetts Institute Of Technology Polymers for functional particles
EP2392583A1 (en) 2006-05-19 2011-12-07 Alnylam Europe AG. RNAi modulation of Aha and therapeutic uses thereof
US7812150B2 (en) 2006-05-19 2010-10-12 Alnylam Pharmaceuticals, Inc. RNAi modulation of Aha and therapeutic uses thereof
US7888498B2 (en) 2006-05-22 2011-02-15 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of IKK-B gene
EP2192200A1 (en) 2006-05-22 2010-06-02 Alnylam Pharmaceuticals, Inc Compositions and methods for inhibiting expression of IKK-B gene
EP2584051A1 (en) 2006-05-22 2013-04-24 Alnylam Pharmaceuticals Inc. Compositions and methods for inhibiting expressions of IKK-B gene
US8017764B2 (en) 2006-06-09 2011-09-13 Quark Pharmaceuticals Inc. Therapeutic uses of inhibitors of RTP801L
US7915399B2 (en) 2006-06-09 2011-03-29 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
US7626015B2 (en) 2006-06-09 2009-12-01 Quark Pharmaceuticals, Inc. Therapeutic uses of inhibitors of RTP801L
US9381477B2 (en) 2006-06-23 2016-07-05 Massachusetts Institute Of Technology Microfluidic synthesis of organic nanoparticles
EP2471815A1 (en) 2006-07-11 2012-07-04 University Of Medicine And Dentistry Of New Jersey Proteins, nucleic acids encoding the same and associated methods of use
EP2471809A1 (en) 2006-07-11 2012-07-04 University Of Medicine And Dentistry Of New Jersey Proteins, nucleic acids encoding the same and associated methods of use
EP2546337A1 (en) 2006-07-21 2013-01-16 Silence Therapeutics AG Means for inhibiting the expression of protein kinase 3
US8232256B2 (en) 2006-07-21 2012-07-31 Silence Therapeutics Ag Means for inhibiting the expression of protein kinase 3
EP2412383A1 (en) 2006-07-28 2012-02-01 Children's Memorial Hospital Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells
US7919613B2 (en) 2006-09-18 2011-04-05 Alnylam Pharmaceuticals, Inc. RNAi modulation of SCAP and therapeutic uses thereof
US7737266B2 (en) 2006-09-18 2010-06-15 Board Of Regents, The University Of Texas System RNAi modulation of SCAP and therapeutics uses thereof
US9102940B2 (en) 2006-09-18 2015-08-11 Alnylam Pharmaceuticals, Inc. RNAi modulation of SCAP and therapeutic uses thereof
US8383805B2 (en) 2006-09-18 2013-02-26 Alnylam Pharmaceuticals, Inc. RNAi modulation of SCAP and therapeutic uses thereof
WO2008036933A2 (en) 2006-09-21 2008-03-27 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the hamp gene
US8470799B2 (en) 2006-09-21 2013-06-25 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the HAMP gene
US9506067B2 (en) 2006-09-21 2016-11-29 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the HAMP gene
US9090895B2 (en) 2006-09-21 2015-07-28 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of the HAMP gene
US9446062B2 (en) 2006-10-25 2016-09-20 Quark Pharmaceuticals, Inc. Methods of treating ischemia-reperfusion injury with siRNAs
EP2431053A1 (en) 2006-11-27 2012-03-21 Patrys Limited Novel glycosylated peptide target in neoplastic cells
EP2913341A1 (en) 2006-12-22 2015-09-02 University of Utah Research Foundation Method of detecting ocular diseases and pathologic conditions and treatment of same
EP2202314A1 (en) 2007-01-15 2010-06-30 BASF Plant Science GmbH Use of subtilisin (RNR9) polynucleotides for achieving a pathogen resistance in plants
WO2008092153A2 (en) 2007-01-26 2008-07-31 University Of Louisville Research Foundation, Inc. Modification of exosomal components for use as a vaccine
US9217129B2 (en) 2007-02-09 2015-12-22 Massachusetts Institute Of Technology Oscillating cell culture bioreactor
WO2008099013A1 (en) 2007-02-16 2008-08-21 Basf Plant Science Gmbh Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants
US7872119B2 (en) 2007-02-26 2011-01-18 Quark Pharmaceuticals, Inc. Inhibitors of RTP801 and their use in disease treatment
EP2474556A2 (en) 2007-03-14 2012-07-11 Novartis AG APCDD1 inhibitors for treating, diagnosing or detecting cancer
US8410069B2 (en) 2007-03-21 2013-04-02 Quark Pharmaceuticals, Inc. Oligoribonucleotide inhibitors of Nrf2 and methods of use thereof for treatment of cancer
US7812002B2 (en) 2007-03-21 2010-10-12 Quark Pharmaceuticals, Inc. Oligoribonucleotide inhibitors of NRF2 and methods of use thereof for treatment of cancer
EP2441837A1 (en) 2007-03-26 2012-04-18 Alnylam Pharmaceuticals Inc. dsRNA compositions and methods for treating HPV infections
EP2905336A1 (en) 2007-03-29 2015-08-12 Alnylam Pharmaceuticals Inc. Compositions and methods for inhibiting expression of a gene from the ebola
EP2589660A1 (en) 2007-03-29 2013-05-08 Alnylam Pharmaceuticals Inc. Compositions and methods for inhibiting expression of a gene from the Ebola
US9333179B2 (en) 2007-04-04 2016-05-10 Massachusetts Institute Of Technology Amphiphilic compound assisted nanoparticles for targeted delivery
EP2377952A1 (en) 2007-04-26 2011-10-19 Ludwig Institute For Cancer Research Methods for diagnosing and treating astrocytomas
EP2607477A1 (en) 2007-05-03 2013-06-26 The Brigham and Women's Hospital, Inc. Multipotent stem cells and uses thereof
DE112008001453T5 (de) 2007-05-22 2010-04-29 Basf Plant Science Gmbh Pflanzenzellen und Pflanzen mit erhöhter Toleranz und/oder Resistenz gegenüber Umweltstress und erhöhter Biomasseproduktion-KO
US8097422B2 (en) 2007-06-20 2012-01-17 Salk Institute For Biological Studies Kir channel modulators
US8785408B2 (en) 2007-06-27 2014-07-22 Quark Pharmaceuticals, Inc. Compositions and methods for reducing or protecting against delayed graft function (DGF)
EP2316943A1 (en) 2007-07-05 2011-05-04 Novartis AG DSRNA for treating viral infection
EP2319926A1 (en) 2007-07-05 2011-05-11 Novartis AG DSRNA for treating viral infection
EP2660333A1 (en) 2007-07-14 2013-11-06 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US9562263B2 (en) 2007-07-14 2017-02-07 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US9562264B2 (en) 2007-07-14 2017-02-07 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US9617586B2 (en) 2007-07-14 2017-04-11 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US10851406B2 (en) 2007-07-14 2020-12-01 Ionian Technologies, Llc Nicking and extension amplification reaction for the exponential amplification of nucleic acids
EP3754029A1 (en) 2007-07-14 2020-12-23 Ionian Technologies, LLC Nicking and extension amplification reaction for the exponential amplification of nucleic acids
EP2657350A1 (en) 2007-07-14 2013-10-30 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US8586726B2 (en) 2007-07-18 2013-11-19 The Trustees Of Columbia University In The City Of New York Tissue-specific MicroRNAs and compositions and uses thereof
US8012474B2 (en) 2007-08-02 2011-09-06 Nov Immune S.A. Anti-RANTES antibodies
US8673299B2 (en) 2007-08-02 2014-03-18 Novimmune S.A. Anti-RANTES antibodies
US8491897B2 (en) 2007-08-23 2013-07-23 The Board Of Trustees Of The Leland Stanford Junior University Treatment of neuropathic pain using anti-thrombospondin antibodies
US8183221B2 (en) 2007-09-05 2012-05-22 Medtronic, Inc. Suppression of SCN9A gene expression and/or function for the treatment of pain
US9080215B2 (en) 2007-09-14 2015-07-14 Asuragen, Inc. MicroRNAs differentially expressed in cervical cancer and uses thereof
US8614309B2 (en) 2007-10-03 2013-12-24 Quark Pharmaceuticals, Inc. Double-stranded RNA directed to CASP2 and methods of use thereof
WO2009044392A2 (en) 2007-10-03 2009-04-09 Quark Pharmaceuticals, Inc. Novel sirna structures
US9539210B2 (en) 2007-10-12 2017-01-10 Massachusetts Institute Of Technology Vaccine nanotechnology
US9526702B2 (en) 2007-10-12 2016-12-27 Massachusetts Institute Of Technology Vaccine nanotechnology
US11547667B2 (en) 2007-10-12 2023-01-10 Massachusetts Institute Of Technology Vaccine nanotechnology
US9474717B2 (en) 2007-10-12 2016-10-25 Massachusetts Institute Of Technology Vaccine nanotechnology
US10736848B2 (en) 2007-10-12 2020-08-11 Massachusetts Institute Of Technology Vaccine nanotechnology
EP3705125A1 (en) 2007-12-04 2020-09-09 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
EP4223299A2 (en) 2007-12-04 2023-08-09 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
EP3141265A1 (en) 2007-12-04 2017-03-15 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
EP4321177A2 (en) 2007-12-04 2024-02-14 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
EP4074344A1 (en) 2007-12-04 2022-10-19 Arbutus Biopharma Corporation Targeting lipids
EP3156077A1 (en) 2007-12-04 2017-04-19 Arbutus Biopharma Corporation Targeting lipids
EP2848688A1 (en) 2007-12-10 2015-03-18 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of factor VII gene
EP2617828A1 (en) 2007-12-10 2013-07-24 Alnylam Pharmaceuticals Inc. Compositions and methods for inhibiting expression of factor VII gene
US8614311B2 (en) 2007-12-12 2013-12-24 Quark Pharmaceuticals, Inc. RTP801L siRNA compounds and methods of use thereof
DE112008003433T5 (de) 2007-12-21 2010-11-04 Basf Plant Science Gmbh Pflanzen mit erhöhtem Ertrag (KO NUE)
EP2712926A2 (en) 2008-03-05 2014-04-02 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of Eg5 and VEGF genes
WO2009123764A2 (en) 2008-04-04 2009-10-08 Calando Pharmaceuticals, Inc. Compositions and use of epas1 inhibitors
EP3604533A1 (en) 2008-04-11 2020-02-05 Arbutus Biopharma Corporation Site-specific delivery of nucleic acids by combining targeting ligands with endosomolytic components
US8278287B2 (en) 2008-04-15 2012-10-02 Quark Pharmaceuticals Inc. siRNA compounds for inhibiting NRF2
US8846868B2 (en) 2008-04-17 2014-09-30 Fusion Antibodies Limited Anti-AREG/HB-EGF antibodies and treatment
US9365852B2 (en) 2008-05-08 2016-06-14 Mirna Therapeutics, Inc. Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
WO2009147684A2 (en) 2008-06-06 2009-12-10 Quark Pharmaceuticals, Inc. Compositions and methods for treatment of ear disorders
US9885036B2 (en) 2008-07-01 2018-02-06 Daiichi Sankyo Company, Limited Double-stranded polynucleotide
EP2690175A2 (en) 2008-09-02 2014-01-29 Alnylam Pharmaceuticals Compositions and methods for combined inhibition of mutant EGFR gene and IL-6 expression
EP3208337A1 (en) 2008-09-02 2017-08-23 Alnylam Pharmaceuticals, Inc. Compositions for combined inhibition of mutant egfr and il-6 expression
EP3587434A1 (en) 2008-09-23 2020-01-01 Alnylam Pharmaceuticals Inc. Chemical modifications of monomers and oligonucleotides with click components for conjugation with ligands
EP3109321A1 (en) 2008-09-25 2016-12-28 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of serum amyloid a gene
EP3584320A1 (en) 2008-09-25 2019-12-25 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of serum amyloid a gene
US8692080B2 (en) 2008-09-29 2014-04-08 Monsanto Technology Llc Soybean transgenic event MON87705 and methods for detection thereof
US8329989B2 (en) 2008-09-29 2012-12-11 Monsanto Technology Llc Soybean transgenic event MON87705 and methods for detection thereof
US9572311B2 (en) 2008-09-29 2017-02-21 Monsanto Technology Llc Soybean transgenic event MON87705 and methods for detection thereof
US10344292B2 (en) 2008-09-29 2019-07-09 Monsanto Technology Llc Soybean transgenic event MON87705 and methods for detection thereof
US9308280B2 (en) 2008-10-12 2016-04-12 Massachusetts Institute Of Technology Targeting of antigen presenting cells with immunonanotherapeutics
US8932595B2 (en) 2008-10-12 2015-01-13 Massachusetts Institute Of Technology Nicotine immunonanotherapeutics
US8906381B2 (en) 2008-10-12 2014-12-09 Massachusetts Institute Of Technology Immunonanotherapeutics that provide IGG humoral response without T-cell antigen
US9439859B2 (en) 2008-10-12 2016-09-13 Massachusetts Institute Of Technology Adjuvant incorporation in immunoanotherapeutics
EP3848461A1 (en) 2008-10-20 2021-07-14 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of transthyretin
EP3354733A1 (en) 2008-10-20 2018-08-01 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of transthyretin
EP2937418A1 (en) 2008-10-20 2015-10-28 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of transthyretin
EP2727996A1 (en) 2008-11-06 2014-05-07 The Johns-Hopkins University Treatment of chronic inflammatory respiratory disorders with NP1 inhibitors
WO2010054401A1 (en) 2008-11-10 2010-05-14 Alnylam Pharmaceuticals, Inc. Novel lipids and compositions for the delivery of therapeutics
EP4241767A2 (en) 2008-11-10 2023-09-13 Arbutus Biopharma Corporation Novel lipids and compositions for the delivery of therapeutics
EP3238738A1 (en) 2008-11-10 2017-11-01 Arbutus Biopharma Corporation Novel lipids and compositions for the delivery of therapeutics
EP3699172A2 (en) 2008-11-10 2020-08-26 Arbutus Biopharma Corporation Novel lipids and compositions for the delivery of therapeutics
WO2010054406A1 (en) 2008-11-10 2010-05-14 Alnylam Pharmaceuticals, Inc. Novel lipids and compositions for the delivery of therapeutics
EP3757090A1 (en) 2008-11-10 2020-12-30 Arbutus Biopharma Corporation Novel lipids and compositions for the delivery of therapeutics
EP2687609A2 (en) 2008-11-10 2014-01-22 The United States of America As Represented by the Secretary Department of Health and Human Services Method for treating solid tumor
EP4495237A2 (en) 2008-11-10 2025-01-22 Arbutus Biopharma Corporation Novel lipids and compositions for the delivery of therapeutics
US8470792B2 (en) 2008-12-04 2013-06-25 Opko Pharmaceuticals, Llc. Compositions and methods for selective inhibition of VEGF
EP3225281A1 (en) 2008-12-10 2017-10-04 Alnylam Pharmaceuticals, Inc. Gnaq targeted dsrna compositions and methods for inhibiting expression
WO2010068816A1 (en) 2008-12-10 2010-06-17 Alnylam Pharmaceuticals, Inc. Gnaq targeted dsrna compositions and methods for inhibiting expression
WO2010080452A2 (en) 2008-12-18 2010-07-15 Quark Pharmaceuticals, Inc. siRNA COMPOUNDS AND METHODS OF USE THEREOF
US8697875B2 (en) 2008-12-23 2014-04-15 The Trustees Of Columbia University In The City Of New York Phosphodiesterase inhibitors and uses thereof
WO2010074783A1 (en) 2008-12-23 2010-07-01 The Trustees Of Columbia University In The City Of New York Phosphodiesterase inhibitors and uses thereof
US9974782B2 (en) 2008-12-23 2018-05-22 The Trustees Of Columbia University In The City Of New York Phosphodiesterase inhibitors and uses thereof
US9422242B2 (en) 2008-12-23 2016-08-23 The Trustees Of Columbia University In The City Of New York Phosphodiesterase inhibitors and uses thereof
WO2010099341A1 (en) 2009-02-26 2010-09-02 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of mig-12 gene
EP3424939A1 (en) 2009-03-02 2019-01-09 Alnylam Pharmaceuticals Inc. Nucleic acid chemical modifications
EP2669290A1 (en) 2009-03-02 2013-12-04 Alnylam Pharmaceuticals Inc. Nucleic Acid Chemical Modifications
WO2010101951A1 (en) 2009-03-02 2010-09-10 Alnylam Pharmaceuticals, Inc. Nucleic acid chemical modifications
WO2010105209A1 (en) 2009-03-12 2010-09-16 Alnylam Pharmaceuticals, Inc. LIPID FORMULATED COMPOSITIONS AND METHODS FOR INHIBITING EXPRESSION OF Eg5 AND VEGF GENES
WO2010107955A2 (en) 2009-03-19 2010-09-23 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF BTB AND CNC HOMOLOGY 1, BASIC LEUCINE ZIPPER TRANSCRIPTION FACTOR 1 (BACH 1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA) SEQUENCE LISTING
WO2010107952A2 (en) 2009-03-19 2010-09-23 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF CONNECTIVE TISSUE GROWTH FACTOR (CTGF) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010107958A1 (en) 2009-03-19 2010-09-23 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION 6 (STAT6) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010107957A2 (en) 2009-03-19 2010-09-23 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF GATA BINDING PROTEIN 3 (GATA3) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010111464A1 (en) 2009-03-27 2010-09-30 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF APOPTOSIS SIGNAL-REGULATING KINASE 1 (ASK1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010111471A2 (en) 2009-03-27 2010-09-30 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION 1 (STAT1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010111490A2 (en) 2009-03-27 2010-09-30 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF THE THYMIC STROMAL LYMPHOPOIETIN (TSLP) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010111497A2 (en) 2009-03-27 2010-09-30 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF THE INTERCELLULAR ADHESION MOLECULE 1 (ICAM-1)GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
WO2010111468A2 (en) 2009-03-27 2010-09-30 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF THE NERVE GROWTH FACTOR BETA CHAIN (NGFß) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (SINA)
WO2010118243A2 (en) 2009-04-08 2010-10-14 Genentech, Inc. Use of il-27 antagonists to treat lupus
US8283332B2 (en) 2009-04-17 2012-10-09 University Of Louisville Research Foundation, Inc. PFKFB4 inhibitors and methods of using the same
WO2010122110A1 (en) 2009-04-22 2010-10-28 Basf Plant Science Company Gmbh Whole seed specific promoter
DE112010003162T5 (de) 2009-04-22 2012-08-16 Basf Plant Science Company Gmbh Gesamtsamen-spezifischer Promotor
EP2258858A1 (en) 2009-06-05 2010-12-08 Universitätsklinikum Freiburg Transgenic LSD1 animal model for cancer
EP3431076A1 (en) 2009-06-10 2019-01-23 Arbutus Biopharma Corporation Improved lipid formulation
WO2010144740A1 (en) 2009-06-10 2010-12-16 Alnylam Pharmaceuticals, Inc. Improved lipid formulation
US9051567B2 (en) 2009-06-15 2015-06-09 Tekmira Pharmaceuticals Corporation Methods for increasing efficacy of lipid formulated siRNA
US10053689B2 (en) 2009-06-15 2018-08-21 Arbutus Biopharma Corporation Methods for increasing efficacy of lipid formulated siRNA
US8273869B2 (en) 2009-06-15 2012-09-25 Alnylam Pharmaceuticals, Inc. Lipid formulated dsRNA targeting the PCSK9 gene
WO2011003901A1 (en) 2009-07-10 2011-01-13 Basf Plant Science Company Gmbh Expression cassettes for endosperm-specific expression in plants
EP3000481A2 (en) 2009-07-14 2016-03-30 Mayo Foundation for Medical Education and Research Peptide-mediated non-covalent delivery of active agent agents across the blood brain barrier
US9597408B2 (en) 2009-07-14 2017-03-21 Mayo Foundation For Medical Education And Research Peptide-mediated non-covalent delivery of active agents across the blood-brain barrier
US10377813B2 (en) 2009-07-14 2019-08-13 Mayo Foundation For Medical Education And Research Peptide-mediated non-covalent delivery of active agents across the blood-brain barrier
WO2011008823A2 (en) 2009-07-14 2011-01-20 Mayo Foundation For Medical Education And Research Peptide-mediated non-covalent delivery of active agents across the blood brain barrier
WO2011011775A1 (en) 2009-07-24 2011-01-27 The Regents Of The University Of California Methods and compositions for treating and preventing disease associated with avb5 integrin
EP2810643A2 (en) 2009-08-14 2014-12-10 Alnylam Pharmaceuticals Inc. Lipid formulated compositions and mehods for inhibiting expression of a gene from the ebola virus
WO2011028218A1 (en) 2009-09-02 2011-03-10 Alnylam Pharmaceuticals, Inc. Process for triphosphate oligonucleotide synthesis
WO2011063409A1 (en) 2009-11-23 2011-05-26 Aquabounty Technologies, Inc. Maternally induced sterility in animals
US8796239B2 (en) 2009-11-26 2014-08-05 Quark Pharmaceuticals, Inc. Sirna compounds comprising terminal substitutions
US10494631B2 (en) 2009-11-26 2019-12-03 Quark Pharmaceuticals, Inc. siRNA compounds comprising terminal substitutions
US9701960B2 (en) 2009-11-26 2017-07-11 Quark Pharmaceuticals, Inc. siRNA compounds comprising terminal substitutions
WO2011066475A1 (en) 2009-11-26 2011-06-03 Quark Pharmaceuticals, Inc. Sirna compounds comprising terminal substitutions
EP3002332A2 (en) 2009-12-03 2016-04-06 BASF Plant Science Company GmbH Expression cassettes for embryo-specific expression in plants
WO2011067712A1 (en) 2009-12-03 2011-06-09 Basf Plant Science Company Gmbh Expression cassettes for embryo-specific expression in plants
EP3296398A1 (en) 2009-12-07 2018-03-21 Arbutus Biopharma Corporation Compositions for nucleic acid delivery
WO2011071860A2 (en) 2009-12-07 2011-06-16 Alnylam Pharmaceuticals, Inc. Compositions for nucleic acid delivery
WO2011071916A2 (en) 2009-12-07 2011-06-16 The Johns Hopkins University Sr-bi as a predictor of human female infertility and responsiveness to treatment
US9206424B2 (en) 2009-12-09 2015-12-08 Nitto Denko Corporation Modulation of HSP47 expression
EP2862929A1 (en) 2009-12-09 2015-04-22 Quark Pharmaceuticals, Inc. Compositions and methods for treating diseases, disorders or injury of the CNS
US10093923B2 (en) 2009-12-09 2018-10-09 Nitto Denko Corporation Modulation of HSP47 expression
EP3012324A2 (en) 2009-12-09 2016-04-27 Nitto Denko Corporation Modulation of hsp47 expression
EP3434773A2 (en) 2009-12-09 2019-01-30 Nitto Denko Corporation Modulation of hsp47 expression
WO2011072091A1 (en) 2009-12-09 2011-06-16 Quark Pharmaceuticals, Inc. Methods and compositions for treating diseases, disorders or injury of the cns
US8710209B2 (en) 2009-12-09 2014-04-29 Nitto Denko Corporation Modulation of HSP47 expression
US11034647B2 (en) 2009-12-10 2021-06-15 The Trustees Of Columbia University In The City Of New York Histone acetyltransferase activators and uses thereof
US10640457B2 (en) 2009-12-10 2020-05-05 The Trustees Of Columbia University In The City Of New York Histone acetyltransferase activators and uses thereof
EP3632901A1 (en) 2009-12-10 2020-04-08 The Trustees of Columbia University in the City of New York Histone acetyltransferase activators and uses thereof
WO2011075656A1 (en) 2009-12-18 2011-06-23 The University Of British Columbia Methods and compositions for delivery of nucleic acids
EP3494963A1 (en) 2009-12-18 2019-06-12 The University of British Columbia Methods and compositions for delivery of nucleic acids
US9301923B2 (en) 2009-12-23 2016-04-05 Novartis Ag Lipids, lipid compositions, and methods of using them
WO2011076807A2 (en) 2009-12-23 2011-06-30 Novartis Ag Lipids, lipid compositions, and methods of using them
EP3721943A1 (en) 2009-12-23 2020-10-14 Novartis AG Lipids, lipid compositions and methods of using them
WO2011084193A1 (en) 2010-01-07 2011-07-14 Quark Pharmaceuticals, Inc. Oligonucleotide compounds comprising non-nucleotide overhangs
WO2011085056A1 (en) 2010-01-07 2011-07-14 Quark Pharmaceuticals, Inc. Oligonucleotide compounds comprising non-nucleotide overhangs
WO2011094345A1 (en) 2010-01-26 2011-08-04 National Jewish Health Methods and compositions for risk prediction, diagnosis, prognosis, and treatment of pulmonary disorders
WO2011100131A2 (en) 2010-01-28 2011-08-18 Alnylam Pharmacuticals, Inc. Monomers and oligonucleotides comprising cycloaddition adduct(s)
WO2011094580A2 (en) 2010-01-28 2011-08-04 Alnylam Pharmaceuticals, Inc. Chelated copper for use in the preparation of conjugated oligonucleotides
EP3329924A1 (en) 2010-03-29 2018-06-06 Alnylam Pharmaceuticals, Inc. Sirna therapy for transthyretin (ttr) related ocular amyloidosis
WO2011123621A2 (en) 2010-04-01 2011-10-06 Alnylam Pharmaceuticals Inc. 2' and 5' modified monomers and oligonucleotides
US8507663B2 (en) 2010-04-06 2013-08-13 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of CD274/PD-L1 gene
EP4385568A2 (en) 2010-04-06 2024-06-19 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of cd274/pd-l1 gene
US10745704B2 (en) 2010-04-06 2020-08-18 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of CD274/PD-L1 gene
US9932593B2 (en) 2010-04-06 2018-04-03 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibiting expression of CD274/PD-L1 gene
US9422562B2 (en) 2010-04-06 2016-08-23 Alnylam Pharmaceuticals Compositions and methods for inhibiting expression of CD274/PD-L1 gene
WO2011133876A2 (en) 2010-04-22 2011-10-27 Alnylam Pharmaceuticals, Inc. Oligonucleotides comprising acyclic and abasic nucleosides and analogs
WO2011133868A2 (en) 2010-04-22 2011-10-27 Alnylam Pharmaceuticals, Inc. Conformationally restricted dinucleotide monomers and oligonucleotides
WO2011133931A1 (en) 2010-04-22 2011-10-27 Genentech, Inc. Use of il-27 antagonists for treating inflammatory bowel disease
WO2011133871A2 (en) 2010-04-22 2011-10-27 Alnylam Pharmaceuticals, Inc. 5'-end derivatives
US9725479B2 (en) 2010-04-22 2017-08-08 Ionis Pharmaceuticals, Inc. 5′-end derivatives
WO2011145777A1 (ko) 2010-05-20 2011-11-24 광주과학기술원 ＨＩＦ－２α 억제제를 유효성분으로 포함하는 관절염 예방 또는 치료용 약제학적 조성물
WO2011146938A1 (en) 2010-05-21 2011-11-24 NanoOncology, Inc. Reagents and methods for treating cancer
EP3190187A1 (en) 2010-05-21 2017-07-12 Peptimed, Inc. Reagents and methods for treating cancer
US9764031B2 (en) 2010-05-26 2017-09-19 Selecta Biosciences, Inc. Dose selection of adjuvanted synthetic nanocarriers
US9066978B2 (en) 2010-05-26 2015-06-30 Selecta Biosciences, Inc. Dose selection of adjuvanted synthetic nanocarriers
EP3456827A2 (en) 2010-06-02 2019-03-20 Alnylam Pharmaceuticals, Inc. Compositions and methods directed to treating liver fibrosis
WO2011163121A1 (en) 2010-06-21 2011-12-29 Alnylam Pharmaceuticals, Inc. Multifunctional copolymers for nucleic acid delivery
WO2011163466A1 (en) 2010-06-23 2011-12-29 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Regulation of skin pigmentation by neuregulin-1 (nrg-1)
WO2012016188A2 (en) 2010-07-30 2012-02-02 Alnylam Pharmaceuticals, Inc. Methods and compositions for delivery of active agents
WO2012016184A2 (en) 2010-07-30 2012-02-02 Alnylam Pharmaceuticals, Inc. Methods and compositions for delivery of active agents
WO2012018754A2 (en) 2010-08-02 2012-02-09 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF CATENIN (CADHERIN-ASSOCIATED PROTEIN), BETA 1 (CTNNB1) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
EP3330377A1 (en) 2010-08-02 2018-06-06 Sirna Therapeutics, Inc. Rna interference mediated inhibition of catenin (cadherin-associated protein), beta 1 (ctnnb1) gene expression using short interfering nucleic acid (sina)
EP4079856A1 (en) 2010-08-17 2022-10-26 Sirna Therapeutics, Inc. Rna interference mediated inhibition of hepatitis b virus (hbv) gene expression using short interfering nucleic acid (sina)
WO2012024170A2 (en) 2010-08-17 2012-02-23 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF HEPATITIS B VIRUS (HBV) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US9845466B2 (en) 2010-08-24 2017-12-19 Sirna Therapeutics, Inc. Single-stranded RNAi agents containing an internal, non-nucleic acid spacer
EP3372684A1 (en) 2010-08-24 2018-09-12 Sirna Therapeutics, Inc. Single-stranded rnai agents containing an internal, non-nucleic acid spacer
US9243246B2 (en) 2010-08-24 2016-01-26 Sirna Therapeutics, Inc. Single-stranded RNAi agents containing an internal, non-nucleic acid spacer
US10584335B2 (en) 2010-08-24 2020-03-10 Sirna Therapeutics, Inc. Single-stranded RNAi agents containing an internal, non-nucleic acid spacer
WO2012027206A1 (en) 2010-08-24 2012-03-01 Merck Sharp & Dohme Corp. SINGLE-STRANDED RNAi AGENTS CONTAINING AN INTERNAL, NON-NUCLEIC ACID SPACER
WO2012027467A1 (en) 2010-08-26 2012-03-01 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF PROLYL HYDROXYLASE DOMAIN 2 (PHD2) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US9994915B2 (en) 2010-10-11 2018-06-12 Sanford-Burnham Medical Research Institute miR-211 expression and related pathways in human melanoma
US10912816B2 (en) 2010-10-15 2021-02-09 The Trustees Of Columbia University In The City Of New York Obesity-related genes and their proteins and uses thereof
WO2012051567A2 (en) 2010-10-15 2012-04-19 The Trustees Of Columbia University In The City Of New York Obesity-related genes and their proteins and uses thereof
EP3327125A1 (en) 2010-10-29 2018-05-30 Sirna Therapeutics, Inc. Rna interference mediated inhibition of gene expression using short interfering nucleic acids (sina)
US9260471B2 (en) 2010-10-29 2016-02-16 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
WO2012058210A1 (en) 2010-10-29 2012-05-03 Merck Sharp & Dohme Corp. RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACIDS (siNA)
US9970005B2 (en) 2010-10-29 2018-05-15 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US11932854B2 (en) 2010-10-29 2024-03-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
US11193126B2 (en) 2010-10-29 2021-12-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using short interfering nucleic acids (siNA)
EP3766975A1 (en) 2010-10-29 2021-01-20 Sirna Therapeutics, Inc. Rna interference mediated inhibition of gene expression using short interfering nucleic acid (sina)
WO2012061443A2 (en) 2010-11-01 2012-05-10 NanoOncology, Inc. Compositions of a peptide-based system for cell-specific targeting
US9198911B2 (en) 2010-11-02 2015-12-01 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
US9737469B2 (en) 2010-11-02 2017-08-22 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
US11806555B2 (en) 2010-11-02 2023-11-07 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
US9895301B2 (en) 2010-11-02 2018-02-20 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
US11298570B2 (en) 2010-11-02 2022-04-12 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
US10265258B2 (en) 2010-11-02 2019-04-23 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
US10994157B2 (en) 2010-11-02 2021-05-04 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
US9763866B2 (en) 2010-11-02 2017-09-19 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
US9730877B2 (en) 2010-11-02 2017-08-15 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
WO2012061537A2 (en) 2010-11-02 2012-05-10 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
US12138490B2 (en) 2010-11-02 2024-11-12 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
WO2012064824A1 (en) 2010-11-09 2012-05-18 Alnylam Pharmaceuticals, Inc. Lipid formulated compositions and methods for inhibiting expression of eg5 and vegf genes
WO2012078536A2 (en) 2010-12-06 2012-06-14 Quark Pharmaceuticals, Inc. Double stranded oligonucleotide compounds comprising positional modifications
WO2012078209A1 (en) 2010-12-06 2012-06-14 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Diagnosis and treatment of adrenocortical tumors using human microrna-483
US9969677B2 (en) 2010-12-22 2018-05-15 The Trustees Of Columbia University In The City Of New York Histone acetyltransferase modulators and uses thereof
WO2012090150A2 (en) 2010-12-27 2012-07-05 Compugen Ltd New cell-penetrating peptides and uses thereof
WO2012099755A1 (en) 2011-01-11 2012-07-26 Alnylam Pharmaceuticals, Inc. Pegylated lipids and their use for drug delivery
EP3202760A1 (en) 2011-01-11 2017-08-09 Alnylam Pharmaceuticals, Inc. Pegylated lipids and their use for drug delivery
US9487778B2 (en) 2011-03-03 2016-11-08 Quark Pharmaceuticals, Inc. Oligonucleotide modulators of the toll-like receptor pathway
US9796979B2 (en) 2011-03-03 2017-10-24 Quark Pharmaceuticals Inc. Oligonucleotide modulators of the toll-like receptor pathway
WO2012118910A2 (en) 2011-03-03 2012-09-07 Quark Pharmaceuticals, Inc. Compositions and methods for treating lung disease and injury
WO2012118911A1 (en) 2011-03-03 2012-09-07 Quark Pharmaceuticals, Inc. Oligonucleotide modulators of the toll-like receptor pathway
US8841432B2 (en) 2011-04-21 2014-09-23 Snu R&Db Foundation Shuttle vectors for mycobacteria-escherichia coli and uses thereof
US8741867B2 (en) 2011-06-08 2014-06-03 Nitto Denko Corporation Retinoid-liposomes for treating fibrosis
US10195145B2 (en) 2011-06-08 2019-02-05 Nitto Denko Corporation Method for treating fibrosis using siRNA and a retinoid-lipid drug carrier
US10196637B2 (en) 2011-06-08 2019-02-05 Nitto Denko Corporation Retinoid-lipid drug carrier
EP3075855A1 (en) 2011-06-08 2016-10-05 Nitto Denko Corporation Retinoid-liposomes for enhancing modulation of hsp47 expression
US8664376B2 (en) 2011-06-08 2014-03-04 Nitto Denko Corporation Retinoid-liposomes for enhancing modulation of HSP47 expression
US9456984B2 (en) 2011-06-08 2016-10-04 Nitto Denko Corporation Method for treating fibrosis using siRNA and a retinoid-lipid drug carrier
WO2012170957A2 (en) 2011-06-08 2012-12-13 Nitto Denko Corporation Retinoid-liposomes for enhancing modulation of hsp47 expression
US9228188B2 (en) 2011-06-21 2016-01-05 Alnylam Pharmaceuticals, Inc. Compositions and method for inhibiting hepcidin antimicrobial peptide (HAMP) or HAMP-related gene expression
WO2012177947A2 (en) 2011-06-21 2012-12-27 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of expression of apolipoprotein c-iii (apoc3) genes
EP3564393A1 (en) 2011-06-21 2019-11-06 Alnylam Pharmaceuticals, Inc. Assays and methods for determining activity of a therapeutic agent in a subject
EP3693464A2 (en) 2011-06-21 2020-08-12 Alnylam Pharmaceuticals, Inc. Compositions and methods for inhibition of expression of apolipoprotein c-iii (apoc3) genes
EP3388068A1 (en) 2011-06-21 2018-10-17 Alnylam Pharmaceuticals, Inc. Composition and methods for inhibition of expression of protein c (proc) genes
EP4134433A1 (en) 2011-06-23 2023-02-15 Alnylam Pharmaceuticals, Inc. Serpina1 sirnas: compositions of matter and methods of treatment
WO2012178033A2 (en) 2011-06-23 2012-12-27 Alnylam Pharmaceuticals, Inc. Serpina1 sirnas: compositions of matter and methods of treatment
EP3597750A1 (en) 2011-06-23 2020-01-22 Alnylam Pharmaceuticals, Inc. Serpina1 sirnas: compositions of matter and methods of treatment
EP3366312A1 (en) 2011-06-23 2018-08-29 Alnylam Pharmaceuticals, Inc. Serpina 1 sirnas: compositions of matter and methods of treatment
WO2013003697A1 (en) 2011-06-30 2013-01-03 Trustees Of Boston University Method for controlling tumor growth, angiogenesis and metastasis using immunoglobulin containing and proline rich receptor-1 (igpr-1)
US9771430B2 (en) 2011-09-02 2017-09-26 The Trustees Of Columbia University In The City Of New York Method of treating a metabolic disorder induced by obesity in a subject in need thereof by administering MK2/3 inhibitors
EP3072555A1 (en) 2011-09-02 2016-09-28 The Trustees of Columbia University in the City of New York Mk2/3 inhibitors to treat metabolic disturbances of obesity
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
US10655184B2 (en) 2011-09-13 2020-05-19 Interpace Diagnostics, Llc Methods and compositions involving miR-135b for distinguishing pancreatic cancer from benign pancreatic disease
US11033894B2 (en) 2011-09-23 2021-06-15 Abbott Diagnostics Scarborough, Inc. System and apparatus for reactions
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
US10040061B2 (en) 2011-09-23 2018-08-07 Alere Switzerland Gmbh System and apparatus for reactions
US12269026B2 (en) 2011-09-23 2025-04-08 Abbott Diagnostics Scarborough, Inc. System and apparatus for reactions including a liquid transfer device with an asymmetrical cross-section
EP3456317A1 (en) 2011-09-27 2019-03-20 Alnylam Pharmaceuticals, Inc. Di-aliphatic substituted pegylated lipids
WO2013049328A1 (en) 2011-09-27 2013-04-04 Alnylam Pharmaceuticals, Inc. Di-aliphatic substituted pegylated lipids
US9738727B2 (en) 2011-10-14 2017-08-22 Genentech, Inc. Anti-HtrA1 antibodies and methods of use
EP3960726A1 (en) 2011-10-18 2022-03-02 Dicerna Pharmaceuticals, Inc. Amine cationic lipids and uses thereof
WO2013059496A1 (en) 2011-10-18 2013-04-25 Dicerna Pharmaceuticals, Inc. Amine cationic lipids and uses thereof
EP3597644A1 (en) 2011-10-18 2020-01-22 Dicerna Pharmaceuticals, Inc. Amine cationic lipids and uses thereof
WO2013067076A2 (en) 2011-11-03 2013-05-10 Quark Pharmaceuticals, Inc. Methods and compositions for neuroprotection
WO2013070821A1 (en) 2011-11-08 2013-05-16 Quark Pharmaceuticals, Inc. Methods and compositions for treating diseases, disorders or injury of the nervous system
WO2013119880A1 (en) 2012-02-07 2013-08-15 Global Bio Therapeutics Usa, Inc. Compartmentalized method of nucleic acid delivery and compositions and uses thereof
US9821114B2 (en) 2012-02-07 2017-11-21 Global Bio Therapeutics, Inc. Compartmentalized method of nucleic acid delivery and compositions and uses thereof
EP3970734A2 (en) 2012-02-07 2022-03-23 Global Bio Therapeutics, Inc. Compartmentalized method of nucleic acid delivery and compositions and uses thereof
WO2013149194A1 (en) 2012-03-29 2013-10-03 The Trustees Of Columbia University In The City Of New York Methods for treating hair loss disorders
EP3459565A1 (en) 2012-03-29 2019-03-27 The Trustees of Columbia University in the City of New York Methods for treating hair loss disorders
EP3919620A1 (en) 2012-05-02 2021-12-08 Sirna Therapeutics, Inc. Short interfering nucleic acid (sina) compositions
WO2013165816A2 (en) 2012-05-02 2013-11-07 Merck Sharp & Dohme Corp. SHORT INTERFERING NUCLEIC ACID (siNA) COMPOSITIONS
WO2013170960A1 (en) 2012-05-16 2013-11-21 Silence Therapeutics Ag Use of vegfr1 as a biomarker for pkn3 inhibitor administration
US9133515B2 (en) 2012-05-16 2015-09-15 Silence Therapeutics Gmbh Use of VEGFR1 as a biomarker
US9751933B2 (en) 2012-06-14 2017-09-05 Scripps Korea Antibody Institute Antibody specific for CLEC14A and uses thereof
WO2014006227A1 (en) 2012-07-06 2014-01-09 Institut Gustave-Roussy Simultaneous detection of cannibalism and senescence as prognostic marker for cancer
WO2014018554A1 (en) 2012-07-23 2014-01-30 La Jolla Institute For Allergy And Immunology Ptprs and proteoglycans in autoimmune disease
WO2014058915A2 (en) 2012-10-08 2014-04-17 St. Jude Children's Research Hospital Therapies based on control of regulatory t cell stability and function via a neuropilin-1:semaphorin axis
EP3677310A1 (en) 2012-10-08 2020-07-08 St. Jude Children's Research Hospital Therapies based on control of regulatory t cell stability and function via a neuropilin-1:semaphorin axis
WO2014068072A1 (en) 2012-10-31 2014-05-08 Institut Gustave-Roussy Identification, assessment and therapy of essential thrombocythemia with resistance to jak2 inhibitors
US12460206B2 (en) 2012-12-05 2025-11-04 Alnylam Pharmaceuticals, Inc. PCSK9 iRNA compositions and methods of use thereof
EP3608308A1 (en) 2013-03-08 2020-02-12 Novartis AG Lipids and lipid compositions for the delivery of active agents
WO2014136086A1 (en) 2013-03-08 2014-09-12 Novartis Ag Lipids and lipid compositions for the delivery of active agents
EP3699592A1 (en) 2013-03-13 2020-08-26 Geneweave Biosciences Inc. Non-replicative transduction particles and transduction particle-based reporter systems
WO2014151734A1 (en) 2013-03-15 2014-09-25 The Trustees Of Columbia University In The City Of New York Fusion proteins and methods thereof
US10208296B2 (en) 2013-03-15 2019-02-19 The Trustees Of Columbia University In The City Of New York Fusion proteins and methods thereof
WO2014145254A2 (en) 2013-03-15 2014-09-18 Sutter West Bay Hospitals Falz for use as a target for therapies to treat cancer
US11505788B2 (en) 2013-03-15 2022-11-22 The Trustees Of Columbia University In The City Of New York Fusion proteins and methods thereof
EP3708184A1 (en) 2013-03-27 2020-09-16 The General Hospital Corporation Methods and agents for treating alzheimer s disease
WO2014160871A2 (en) 2013-03-27 2014-10-02 The General Hospital Corporation Methods and agents for treating alzheimer's disease
US11141492B2 (en) 2013-07-03 2021-10-12 City Of Hope Anticancer combinations
WO2015015496A1 (en) 2013-07-31 2015-02-05 Qbi Enterprises Ltd. Sphingolipid-polyalkylamine-oligonucleotide compounds
WO2015015498A1 (en) 2013-07-31 2015-02-05 Qbi Enterprises Ltd. Methods of use of sphingolipid polyalkylamine oligonucleotide compounds
US11364032B2 (en) 2013-08-08 2022-06-21 Global Bio Therapeutics, Inc. Clamp device for minimally invasive procedures and uses thereof
WO2015050871A2 (en) 2013-10-04 2015-04-09 Novartis Ag Organic compounds to treat hepatitis b virus
WO2015051366A2 (en) 2013-10-04 2015-04-09 Novartis Ag Novel formats for organic compounds for use in rna interference
US11008570B2 (en) 2013-10-04 2021-05-18 Novartis Ag 3′ end caps for RNAi agents for use in RNA interference
US9988627B2 (en) 2013-10-04 2018-06-05 Novartis Ag Formats for organic compounds for use in RNA interference
WO2015051044A2 (en) 2013-10-04 2015-04-09 Novartis Ag Novel formats for organic compounds for use in rna interference
US10519446B2 (en) 2013-10-04 2019-12-31 Novartis Ag Organic compounds to treat hepatitis B virus
EP3722277A2 (en) 2013-10-04 2020-10-14 Novartis AG 3'end caps for rna-interferring agents for use in rna
WO2015051045A2 (en) 2013-10-04 2015-04-09 Novartis Ag 3'END CAPS FOR RNAi AGENTS FOR USE IN RNA INTERFERENCE
US10227588B2 (en) 2013-10-04 2019-03-12 Novartis Ag 3′end caps for RNAi agents for use in RNA interference
WO2015054451A1 (en) 2013-10-09 2015-04-16 The United States Of America As Represented By The Secretary Department Of Health And Human Services Detection of hepatitis delta virus (hdv) for the diagnosis and treatment of sjögren's syndrome and lymphoma
US10246519B2 (en) 2013-10-11 2019-04-02 Genentech, Inc. NSP4 inhibitors and methods of use
US9975963B2 (en) 2013-10-11 2018-05-22 Genentech, Inc. NSP4 inhibitors and methods of use
WO2015054670A1 (en) 2013-10-11 2015-04-16 Genentech, Inc. Nsp4 inhibitors and methods of use
US11529428B2 (en) 2013-11-11 2022-12-20 Sirna Therapeutics, Inc. Systemic delivery of myostatin short interfering nucleic acids (siNA) conjugated to a lipophilic moiety
US10004814B2 (en) 2013-11-11 2018-06-26 Sirna Therapeutics, Inc. Systemic delivery of myostatin short interfering nucleic acids (siNA) conjugated to a lipophilic moiety
US10729787B2 (en) 2013-11-11 2020-08-04 Sirna Therapeutics, Inc. Systemic delivery of myostatin short interfering nucleic acids (siNA) conjugated to a lipophilic moiety
US10426737B2 (en) 2013-12-19 2019-10-01 Novartis Ag Lipids and lipid compositions for the delivery of active agents
US11013696B2 (en) 2013-12-19 2021-05-25 Novartis Ag Lipids and lipid compositions for the delivery of active agents
US10059655B2 (en) 2013-12-19 2018-08-28 Novartis Ag Lipids and lipid compositions for the delivery of active agents
WO2015095346A1 (en) 2013-12-19 2015-06-25 Novartis Ag Lipids and lipid compositions for the delivery of active agents
WO2015095340A1 (en) 2013-12-19 2015-06-25 Novartis Ag Lipids and lipid compositions for the delivery of active agents
EP4019506A1 (en) 2013-12-19 2022-06-29 Novartis AG Lipids and lipid compositions for the delivery of active agents
EP3872066A1 (en) 2013-12-19 2021-09-01 Novartis AG Lipids and lipid compositions for the delivery of active agents
US11420933B2 (en) 2013-12-19 2022-08-23 Novartis Ag Lipids and lipid compositions for the delivery of active agents
US10906867B2 (en) 2013-12-19 2021-02-02 Novartis Ag Lipids and lipid compositions for the delivery of active agents
US9682123B2 (en) 2013-12-20 2017-06-20 The Trustees Of Columbia University In The City Of New York Methods of treating metabolic disease
US9274117B2 (en) 2013-12-21 2016-03-01 Catholic University Industry Academic Use of SIRT7 as novel cancer therapy target and method for treating cancer using the same
WO2015098113A1 (ja) 2013-12-27 2015-07-02 独立行政法人医薬基盤研究所 悪性腫瘍の治療薬
EP3693384A1 (en) 2014-03-11 2020-08-12 Cellectis Method for generating t-cells compatible for allogenic transplantation
EP4368705A2 (en) 2014-03-11 2024-05-15 Cellectis Method for generating t-cells compatible for allogenic transplantation
KR20150129190A (ko) 2014-05-08 2015-11-19 서울대학교산학협력단 정서장애 및 중독질환 치료를 위한 REV-ERBα 유전자의 용도
KR20150129189A (ko) 2014-05-08 2015-11-19 서울대학교산학협력단 도파민 의존성 신경장애 치료를 위한 REV-ERBα 유전자의 용도
WO2016010840A1 (en) 2014-07-16 2016-01-21 Novartis Ag Method of encapsulating a nucleic acid in a lipid nanoparticle host
US10342761B2 (en) 2014-07-16 2019-07-09 Novartis Ag Method of encapsulating a nucleic acid in a lipid nanoparticle host
EP4223285A2 (en) 2014-07-16 2023-08-09 Novartis AG Method of encapsulating a nucleic acid in a lipid nanoparticle host
US11466061B2 (en) 2014-08-22 2022-10-11 Yingfang Liu Methods and compositions for treating and/or preventing a disease or disorder associated with abnormal level and/or activity of the IFP35 family of proteins
WO2016037053A1 (en) 2014-09-05 2016-03-10 Novartis Ag Lipids and lipid compositions for the delivery of active agents
WO2016044271A2 (en) 2014-09-15 2016-03-24 Children's Medical Center Corporation Methods and compositions to increase somatic cell nuclear transfer (scnt) efficiency by removing histone h3-lysine trimethylation
WO2016057693A1 (en) 2014-10-10 2016-04-14 Alnylam Pharmaceuticals, Inc. Methods and compositions for inhalation delivery of conjugated oligonucleotide
US11111497B2 (en) 2014-11-12 2021-09-07 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
WO2016077624A1 (en) 2014-11-12 2016-05-19 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
US10745708B2 (en) 2014-11-12 2020-08-18 Nmc, Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
EP3778644A2 (en) 2014-12-23 2021-02-17 The Trustees of Columbia University in the City of New York Fgfr-tacc fusion proteins and methods thereof
EP3925979A2 (en) 2014-12-23 2021-12-22 The Trustees of Columbia University in the City of New York Fgfr-tacc fusion proteins and methods thereof
WO2016106400A2 (en) 2014-12-26 2016-06-30 Nitto Denko Corporation Rna interference agents for gst-pi gene modulation
WO2016106402A1 (en) 2014-12-26 2016-06-30 Nitto Denko Corporation Rna interference agents for p21 gene modulation
EP3798308A1 (en) 2014-12-26 2021-03-31 Nitto Denko Corporation Rna interference compositions and methods for malignant tumors
EP3683309A1 (en) 2014-12-26 2020-07-22 Nitto Denko Corporation Rna interference agents for gst-pi gene modulation
WO2016140921A1 (en) 2015-03-02 2016-09-09 180 Therapeutics Lp Method of treating a localized fibrotic disorder using an il-33 antagonist
US10781446B2 (en) 2015-03-09 2020-09-22 University Of Kentucky Research Foundation RNA nanoparticle for treatment of gastric cancer
US11085044B2 (en) 2015-03-09 2021-08-10 University Of Kentucky Research Foundation miRNA for treatment of breast cancer
US11325939B2 (en) 2015-03-09 2022-05-10 University Of Kentucky Research Foundation RNA nanoparticles for brain tumor treatment
US9669103B2 (en) 2015-03-18 2017-06-06 Korea National University Of Transportation Industry-Academic Cooperation Foundation Metal-particle-based oral-administrating liver-specific nucleic acid delivery system and method of manufacturing the same
US11279768B1 (en) 2015-04-03 2022-03-22 Precision Biologics, Inc. Anti-cancer antibodies, combination therapies, and uses thereof
US11964028B2 (en) 2015-04-17 2024-04-23 University Of Kentucky Research Foundation RNA nanoparticles and method of use thereof
WO2016176617A2 (en) 2015-04-29 2016-11-03 New York University Method for treating high-grade gliomas
US11136390B2 (en) 2015-06-12 2021-10-05 Alector Llc Anti-CD33 antibodies and methods of use thereof
US11174313B2 (en) 2015-06-12 2021-11-16 Alector Llc Anti-CD33 antibodies and methods of use thereof
WO2016201388A2 (en) 2015-06-12 2016-12-15 Alector Llc Anti-cd33 antibodies and methods of use thereof
WO2016201389A2 (en) 2015-06-12 2016-12-15 Alector Llc Anti-cd33 antibodies and methods of use thereof
WO2016210292A1 (en) 2015-06-25 2016-12-29 Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion, enrichment, and maintenance
US10072065B2 (en) 2015-08-24 2018-09-11 Mayo Foundation For Medical Education And Research Peptide-mediated delivery of immunoglobulins across the blood-brain barrier
US11390680B2 (en) 2015-08-28 2022-07-19 Alector Llc Anti-Siglec-7 antibodies and methods of use thereof
WO2017040301A1 (en) 2015-08-28 2017-03-09 Alector Llc Anti-siglec-7 antibodies and methods of use thereof
US10590198B2 (en) 2015-08-28 2020-03-17 Alector Llc Anti-siglec-7 antibodies and methods of use thereof
US11685918B2 (en) 2015-09-02 2023-06-27 Alnylam Pharmaceuticals, Inc. Programmed cell death 1 ligand 1 (PD-L1) iRNA compositions and methods of use thereof
US10889813B2 (en) 2015-09-02 2021-01-12 Alnylam Pharmaceuticals, Inc. Programmed cell death 1 ligand 1 (PD-L1) iRNA compositions and methods of use thereof
US11266626B2 (en) 2015-09-09 2022-03-08 The Trustees Of Columbia University In The City Of New York Reduction of ER-MAM-localized APP-C99 and methods of treating alzheimer's disease
EP3702470A2 (en) 2015-09-09 2020-09-02 The Trustees of Columbia University in the City of New York Reduction of er-mam-localized app-c99 and methods of treating alzheimer's disease
US11285142B2 (en) 2015-09-29 2022-03-29 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Compositions and methods for identifying and treating dystonia disorders
US10857145B2 (en) 2015-09-29 2020-12-08 Duke University Compositions and methods for identifying and treating dystonia disorders
US11667710B2 (en) 2015-10-29 2023-06-06 Alector Llc Anti-Siglec-9 antibodies and methods of use thereof
WO2017075432A2 (en) 2015-10-29 2017-05-04 Alector Llc Anti-siglec-9 antibodies and methods of use thereof
US10800844B2 (en) 2015-10-29 2020-10-13 Alector Llc Anti-Siglec-9 antibodies and methods of use thereof
US10421822B2 (en) 2015-10-30 2019-09-24 Genetech, Inc. Anti-HtrA1 antibodies and methods of use thereof
US11512143B2 (en) 2015-10-30 2022-11-29 Genentech, Inc. Anti-HtrA1 antibodies and methods of use thereof
US10421821B2 (en) 2015-10-30 2019-09-24 Genentech, Inc. Anti-HtrA1 antibodies and methods of use thereof
EP3816287A1 (en) 2015-12-13 2021-05-05 Nitto Denko Corporation Sirna structures for high activity and reduced off target
WO2017152073A1 (en) 2016-03-04 2017-09-08 University Of Louisville Research Foundation, Inc. Methods and compositions for ex vivo expansion of very small embryonic-like stem cells (vsels)
EP4049665A1 (en) 2016-03-15 2022-08-31 Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion
WO2017161001A1 (en) 2016-03-15 2017-09-21 Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion
US10883108B2 (en) 2016-03-31 2021-01-05 The Schepens Eye Research Institute, Inc. Endomucin inhibitor as an anti-angiogenic agent
US11680266B2 (en) 2016-03-31 2023-06-20 The Schepens Eye Research Institute, Inc. Endomucin inhibitor as an anti-angiogenic agent
EP4209501A1 (en) 2016-05-13 2023-07-12 4D Molecular Therapeutics Inc. Adeno-associated virus variant capsids and methods of use thereof
EP4206216A1 (en) 2016-05-13 2023-07-05 4D Molecular Therapeutics Inc. Adeno-associated virus variant capsids and methods of use thereof
WO2018020012A1 (en) 2016-07-29 2018-02-01 Danmarks Tekniske Universitet Methods for decoupling cell growth from production of biochemicals and recombinant polypeptides
US10933081B2 (en) 2016-09-21 2021-03-02 Alnylam Pharmaceuticals, Inc. Myostatin iRNA compositions and methods of use thereof
WO2018057575A1 (en) 2016-09-21 2018-03-29 Alnylam Pharmaceuticals, Inc Myostatin irna compositions and methods of use thereof
EP4035659A1 (en) 2016-11-29 2022-08-03 PureTech LYT, Inc. Exosomes for delivery of therapeutic agents
US11147249B2 (en) 2016-12-08 2021-10-19 Alector Llc Siglec transgenic mice and methods of use thereof
US12004494B2 (en) 2016-12-08 2024-06-11 Alector Llc Siglec transgenic mice and methods of use thereof
US11359014B2 (en) 2017-05-16 2022-06-14 Alector Llc Anti-siglec-5 antibodies and methods of use thereof
WO2018213316A1 (en) 2017-05-16 2018-11-22 Alector Llc Anti-siglec-5 antibodies and methods of use thereof
US11965023B2 (en) 2017-05-16 2024-04-23 Alector Llc Anti-Siglec-5 antibodies and methods of use thereof
US10711062B2 (en) 2017-08-03 2020-07-14 Alector Llc Anti-CD33 antibodies and methods of use thereof
US11254743B2 (en) 2017-08-03 2022-02-22 Alector Llc Anti-CD33 antibodies and methods of use thereof
WO2019047914A1 (zh) 2017-09-07 2019-03-14 北京泰德制药股份有限公司 靶向ckip-1的双链rna分子及其用途
EP4218828A2 (en) 2017-09-20 2023-08-02 4D Molecular Therapeutics Inc. Adeno-associated virus variant capsids and methods of use thereof
EP4272728A2 (en) 2017-11-27 2023-11-08 4D Molecular Therapeutics Inc. Adeno-associated virus variant capsids and use for inhibiting angiogenesis
EP4219695A2 (en) 2017-11-27 2023-08-02 4D Molecular Therapeutics Inc. Adeno-associated virus variant capsids and use for inhibiting angiogenesis
US11470827B2 (en) 2017-12-12 2022-10-18 Alector Llc Transgenic mice expressing human TREM proteins and methods of use thereof
WO2019126651A1 (en) 2017-12-21 2019-06-27 Alnylam Pharmaceuticals, Inc. Chirally-enriched double-stranded rna agents
WO2019199673A1 (en) 2018-04-09 2019-10-17 President And Fellows Of Harvard College Modulating nuclear receptors and methods of using same
US12227568B2 (en) 2018-06-08 2025-02-18 Alector Llc Anti-Siglec-7 antibodies and methods of use thereof
US12258398B2 (en) 2018-07-27 2025-03-25 Alector Llc Anti-Siglec-5 antibodies and methods of use thereof
WO2020023920A1 (en) 2018-07-27 2020-01-30 Alector Llc Anti-siglec-5 antibodies and methods of use thereof
US12247073B2 (en) 2018-08-31 2025-03-11 Alector Llc Anti-CD33 antibodies and methods of use thereof
WO2020061381A1 (en) 2018-09-19 2020-03-26 La Jolla Institute For Immunology Ptprs and proteoglycans in rheumatoid arthritis
EP4365289A2 (en) 2018-11-16 2024-05-08 Nitto Denko Corporation Rna interference delivery formulation and methods for malignant tumors
US11708575B2 (en) 2018-11-16 2023-07-25 Nitto Denko Corporation RNA interference delivery formulation and methods for malignant tumors
US12215382B2 (en) 2019-03-01 2025-02-04 The General Hospital Corporation Liver protective MARC variants and uses thereof
WO2021037972A1 (en) 2019-08-27 2021-03-04 Sanofi Compositions and methods for inhibiting pcsk9
WO2021060624A1 (ko) 2019-09-24 2021-04-01 테고사이언스(주) 켈로이드 또는 비후성 반흔 예방 또는 치료용 조성물
WO2021124172A1 (en) 2019-12-18 2021-06-24 Novartis Ag 3-(5-methoxy-1-oxoisoindolin-2-yl)piperidine-2,6-dione derivatives and uses thereof
WO2021123920A1 (en) 2019-12-18 2021-06-24 Novartis Ag Compositions and methods for the treatment of hemoglobinopathies
WO2021142191A1 (en) 2020-01-08 2021-07-15 Regeneron Pharmaceuticals, Inc. Treatment of fibrodysplasia ossificans progressiva
US12178902B1 (en) 2020-01-12 2024-12-31 University Of Southern California Methods and compositions for fluid drainage by Piezo ion channel activation
WO2021150300A1 (en) 2020-01-22 2021-07-29 Massachusetts Institute Of Technology Inducible tissue constructs and uses thereof
WO2021150837A1 (en) 2020-01-22 2021-07-29 Massachusetts Institute Of Technology Inducible tissue constructs and uses thereof
WO2021173965A1 (en) 2020-02-28 2021-09-02 Massachusetts Institute Of Technology Identification of variable influenza residues and uses thereof
US12458604B2 (en) 2020-10-14 2025-11-04 The Trustees Of The University Of Pennsylvania Methods of lipid nanoparticle manufacture and compositions derived therefrom
WO2022147480A1 (en) 2020-12-30 2022-07-07 Ansun Biopharma, Inc. Oncolytic virus encoding sialidase and multispecific immune cell engager
WO2022147481A1 (en) 2020-12-30 2022-07-07 Ansun Biopharma Inc. Combination therapy of an oncolytic virus delivering a foreign antigen and an engineered immune cell expressing a chimeric receptor targeting the foreign antigen
WO2022247917A1 (zh) 2021-05-28 2022-12-01 上海瑞宏迪医药有限公司 衣壳变异的重组腺相关病毒及其应用
WO2022269518A2 (en) 2021-06-23 2022-12-29 Novartis Ag Compositions and methods for the treatment of hemoglobinopathies
WO2023012165A1 (en) 2021-08-02 2023-02-09 Universite De Montpellier Compositions and methods for treating cmt1a or cmt1e diseases with rnai molecules targeting pmp22
US12037407B2 (en) 2021-10-14 2024-07-16 Arsenal Biosciences, Inc. Immune cells having co-expressed shRNAS and logic gate systems
WO2024059618A2 (en) 2022-09-13 2024-03-21 Arsenal Biosciences, Inc. Immune cells having co-expressed tgfbr shrnas
WO2024059824A2 (en) 2022-09-16 2024-03-21 Arsenal Biosciences, Inc. Immune cells with combination gene perturbations
WO2024186656A1 (en) 2023-03-03 2024-09-12 Arsenal Biosciences, Inc. Systems targeting psma and ca9
WO2025199338A1 (en) 2024-03-20 2025-09-25 Arsenal Biosciences, Inc. Systems targeting slc34a2 and tmprss4 and methods of use thereof

Also Published As

Publication number Publication date
JP2008005844A (ja) 2008-01-17
US20130164366A1 (en) 2013-06-27
US20040102408A1 (en) 2004-05-27
DE20023125U8 (de) 2005-01-20
US20130164843A1 (en) 2013-06-27
US8101584B2 (en) 2012-01-24
EP1214945A3 (de) 2002-09-04
US8877726B2 (en) 2014-11-04
US8101742B2 (en) 2012-01-24
CA2359180A1 (en) 2000-08-03
JP2006340732A (ja) 2006-12-21
US20050100907A1 (en) 2005-05-12
US8114851B2 (en) 2012-02-14
US20080182981A1 (en) 2008-07-31
DK1214945T4 (da) 2018-11-05
DK1550719T3 (da) 2009-04-27
US20180179526A1 (en) 2018-06-28
US20140051166A1 (en) 2014-02-20
ATE297463T1 (de) 2005-06-15
EP3018207A1 (de) 2016-05-11
DE19956568A1 (de) 2000-08-17
JP2007312784A (ja) 2007-12-06
AU3271300A (en) 2000-08-18
DK1798285T3 (en) 2017-07-03
JP2015146823A (ja) 2015-08-20
DE10080167D2 (de) 2002-02-28
DE50015501D1 (de) 2009-02-05
ES2243608T5 (es) 2019-03-21
US9902955B2 (en) 2018-02-27
US20080261303A1 (en) 2008-10-23
EP1214945B1 (de) 2005-06-08
US20040072779A1 (en) 2004-04-15
US20080233651A1 (en) 2008-09-25
DE20023125U1 (de) 2003-05-15
JP2010057514A (ja) 2010-03-18
JP2007314555A (ja) 2007-12-06
US8183362B2 (en) 2012-05-22
DE10066235C5 (de) 2014-08-21
EP2363479A1 (de) 2011-09-07
ES2597953T3 (es) 2017-01-24
EP1214945B2 (de) 2018-10-10
JP2009102380A (ja) 2009-05-14
JP2009100758A (ja) 2009-05-14
US20040053875A1 (en) 2004-03-18
DK1214945T3 (da) 2005-09-26
JP2010057515A (ja) 2010-03-18
ATE222953T1 (de) 2002-09-15
CA2359180C (en) 2007-06-12
CY1108896T1 (el) 2014-07-02
PT1798285T (pt) 2017-06-26
JP2007031443A (ja) 2007-02-08
US8119608B2 (en) 2012-02-21
DK2363479T3 (en) 2016-10-31
CY1119221T1 (el) 2018-02-14
ES2182791T3 (es) 2003-03-16
US20130177631A1 (en) 2013-07-11
US8168776B2 (en) 2012-05-01
US20080171861A1 (en) 2008-07-17
US8729037B2 (en) 2014-05-20
EP1144623B1 (de) 2002-08-28
US20080166800A1 (en) 2008-07-10
EP1798285B1 (de) 2017-03-15
US8202980B2 (en) 2012-06-19
JP6471901B2 (ja) 2019-02-20
ES2628535T3 (es) 2017-08-03
JP2019017390A (ja) 2019-02-07
CY1118264T1 (el) 2017-06-28
EP1144623A1 (de) 2001-10-17
PT1214945E (pt) 2005-10-31
US20080171862A1 (en) 2008-07-17
EP2363479B1 (de) 2016-07-13
EP1550719A1 (de) 2005-07-06
AU778474B2 (en) 2004-12-09
DE50010528D1 (de) 2005-07-14
PT2363479T (pt) 2016-10-18
ES2321409T3 (es) 2009-06-05
EP1798285A1 (de) 2007-06-20
DE10066235B4 (de) 2008-04-10
DE10080167B4 (de) 2008-03-20
JP2009100757A (ja) 2009-05-14
US9902954B2 (en) 2018-02-27
US8114981B2 (en) 2012-02-14
EP1144623B9 (de) 2003-03-05
PT1550719E (pt) 2009-03-13
EP1214945A2 (de) 2002-06-19
JP2003502012A (ja) 2003-01-21
HK1160487A1 (zh) 2012-08-17
AU2005201044A1 (en) 2005-04-07
US20140220113A1 (en) 2014-08-07
US20140220676A1 (en) 2014-08-07
US9133454B2 (en) 2015-09-15
ATE418610T1 (de) 2009-01-15
DE50000414D1 (de) 2002-10-02
ZA200105909B (en) 2002-07-24
ES2243608T3 (es) 2005-12-01
JP5990468B2 (ja) 2016-09-14
AU2005201044B2 (en) 2008-05-29
JP2013074901A (ja) 2013-04-25
EP1550719B1 (de) 2008-12-24

Similar Documents

Publication Publication Date Title
EP1144623B9 (de) Verfahren und medikament zur hemmung der expression eines vorgegebenen gens
AU2008202208B2 (en) Method and medicament for inhibiting the expression of a defined gene
AU2017276342A1 (en) Method and medicament for inhibiting the expression of a defined gene

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2000910510

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: IN/PCT/2001/00823/MU

Country of ref document: IN

ENP Entry into the national phase

Ref document number: 2359180

Country of ref document: CA

Ref document number: 2359180

Country of ref document: CA

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2001/05909

Country of ref document: ZA

Ref document number: 200105909

Country of ref document: ZA

WWE Wipo information: entry into national phase

Ref document number: 32713/00

Country of ref document: AU

ENP Entry into the national phase

Ref document number: 2000 596137

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 09889802

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2000910510

Country of ref document: EP

REF Corresponds to

Ref document number: 10080167

Country of ref document: DE

Date of ref document: 20020228

WWE Wipo information: entry into national phase

Ref document number: 10080167

Country of ref document: DE

WWG Wipo information: grant in national office

Ref document number: 2000910510

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8607

REG Reference to national code

Ref country code: DE

Ref legal event code: 8607

REG Reference to national code

Ref country code: DE

Ref legal event code: 8607