WO2002059257A3 - Method and means for producing barley yellow dwarf virus resistant cereal plants - Google Patents

Method and means for producing barley yellow dwarf virus resistant cereal plants Download PDF

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Publication number
WO2002059257A3
WO2002059257A3 PCT/IB2001/002737 IB0102737W WO02059257A3 WO 2002059257 A3 WO2002059257 A3 WO 2002059257A3 IB 0102737 W IB0102737 W IB 0102737W WO 02059257 A3 WO02059257 A3 WO 02059257A3
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WO
WIPO (PCT)
Prior art keywords
bydv
rna
dna molecule
resistance
dwarf virus
Prior art date
Application number
PCT/IB2001/002737
Other languages
French (fr)
Other versions
WO2002059257A2 (en
Inventor
Peter Waterhouse
Ming-Bo Wang
David Abbott
Original Assignee
Commw Scient Ind Res Org
Grains Res & Dev Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commw Scient Ind Res Org, Grains Res & Dev Corp filed Critical Commw Scient Ind Res Org
Priority to CA2427347A priority Critical patent/CA2427347C/en
Priority to AU2001297906A priority patent/AU2001297906B2/en
Publication of WO2002059257A2 publication Critical patent/WO2002059257A2/en
Publication of WO2002059257A3 publication Critical patent/WO2002059257A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/00022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a DNA molecule comprising a plant-operable promoter operably linked to a DNA region that is capable of being transcribed in the cells of a cereal plant to produce RNA comprising inverted repeat sequence least about 19 nucleotides from the sequence of an RNA dependent RNA polymerase gene of Barley Yellow Dwarf Virus (BYDV). The DNA molecule is useful for reducing expression of the viral RNA dependant RNA polymerase and for enhancing the resistance of cereal crops to BYDV, optionally in the presence of a viral promoter that normally inactivates other modes of post transcriptional gene silencing. Methods are provided for producing transgenic cereal plant lines comprising the DNA molecule of the invention integrated into their genome, and selecting those lines having resistance to BYDV infection. Transgenic plants having enhanced resistance to BYDV are also provided.
PCT/IB2001/002737 2000-10-31 2001-10-31 Method and means for producing barley yellow dwarf virus resistant cereal plants WO2002059257A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA2427347A CA2427347C (en) 2000-10-31 2001-10-31 Method and means for producing barley yellow dwarf virus resistant cereal plants
AU2001297906A AU2001297906B2 (en) 2000-10-31 2001-10-31 Method and means for producing barley yellow dwarf virus resistant cereal plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24420900P 2000-10-31 2000-10-31
US60/244,209 2000-10-31

Publications (2)

Publication Number Publication Date
WO2002059257A2 WO2002059257A2 (en) 2002-08-01
WO2002059257A3 true WO2002059257A3 (en) 2002-12-19

Family

ID=22921818

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2001/002737 WO2002059257A2 (en) 2000-10-31 2001-10-31 Method and means for producing barley yellow dwarf virus resistant cereal plants

Country Status (4)

Country Link
US (1) US6777588B2 (en)
AU (1) AU2001297906B2 (en)
CA (1) CA2427347C (en)
WO (1) WO2002059257A2 (en)

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Also Published As

Publication number Publication date
AU2001297906B2 (en) 2007-07-19
CA2427347C (en) 2011-01-18
WO2002059257A2 (en) 2002-08-01
US6777588B2 (en) 2004-08-17
US20020169298A1 (en) 2002-11-14
CA2427347A1 (en) 2002-08-01

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