NO840200L - Glukoamylase cdna. - Google Patents
Glukoamylase cdna.Info
- Publication number
- NO840200L NO840200L NO840200A NO840200A NO840200L NO 840200 L NO840200 L NO 840200L NO 840200 A NO840200 A NO 840200A NO 840200 A NO840200 A NO 840200A NO 840200 L NO840200 L NO 840200L
- Authority
- NO
- Norway
- Prior art keywords
- glucoamylase
- host
- dna
- yeast
- sequence
- Prior art date
Links
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 title claims description 222
- 102100022624 Glucoamylase Human genes 0.000 title claims description 153
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 93
- 239000012634 fragment Substances 0.000 claims description 75
- 108090000623 proteins and genes Proteins 0.000 claims description 75
- 108020004414 DNA Proteins 0.000 claims description 64
- 229920002472 Starch Polymers 0.000 claims description 58
- 235000019698 starch Nutrition 0.000 claims description 58
- 239000008107 starch Substances 0.000 claims description 54
- 241001513093 Aspergillus awamori Species 0.000 claims description 51
- 239000013612 plasmid Substances 0.000 claims description 48
- 239000002299 complementary DNA Substances 0.000 claims description 41
- 238000000034 method Methods 0.000 claims description 38
- 102000004169 proteins and genes Human genes 0.000 claims description 38
- 241000588724 Escherichia coli Species 0.000 claims description 35
- 230000000694 effects Effects 0.000 claims description 34
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 31
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 29
- 239000013598 vector Substances 0.000 claims description 29
- 239000013604 expression vector Substances 0.000 claims description 28
- 102000004190 Enzymes Human genes 0.000 claims description 24
- 108090000790 Enzymes Proteins 0.000 claims description 24
- 230000002538 fungal effect Effects 0.000 claims description 21
- 241000894007 species Species 0.000 claims description 16
- 229910052799 carbon Inorganic materials 0.000 claims description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 14
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 13
- 238000000855 fermentation Methods 0.000 claims description 13
- 230000004151 fermentation Effects 0.000 claims description 13
- 238000004519 manufacturing process Methods 0.000 claims description 13
- 239000001963 growth medium Substances 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 8
- 241000228245 Aspergillus niger Species 0.000 claims description 7
- 230000028327 secretion Effects 0.000 claims description 7
- 150000001413 amino acids Chemical group 0.000 claims description 6
- 230000009466 transformation Effects 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 241000235070 Saccharomyces Species 0.000 claims description 3
- 241000700605 Viruses Species 0.000 claims description 3
- 230000037429 base substitution Effects 0.000 claims description 3
- 230000001413 cellular effect Effects 0.000 claims description 3
- 238000012217 deletion Methods 0.000 claims description 3
- 230000037430 deletion Effects 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 229920001542 oligosaccharide Polymers 0.000 claims description 2
- 150000002482 oligosaccharides Chemical class 0.000 claims description 2
- KMFPQTITXUKJOV-DCAQKATOSA-N Arg-Ser-Leu Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O KMFPQTITXUKJOV-DCAQKATOSA-N 0.000 claims 1
- JNCRAQVYJZGIOW-QSFUFRPTSA-N Asn-Val-Ile Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O JNCRAQVYJZGIOW-QSFUFRPTSA-N 0.000 claims 1
- PAWIVEIWWYGBAM-YUMQZZPRSA-N Gly-Leu-Ala Chemical compound NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O PAWIVEIWWYGBAM-YUMQZZPRSA-N 0.000 claims 1
- LSPYFSHXDAYVDI-SRVKXCTJSA-N Leu-Ala-Leu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CC(C)C LSPYFSHXDAYVDI-SRVKXCTJSA-N 0.000 claims 1
- FIZZULTXMVEIAA-IHRRRGAJSA-N Met-Ser-Phe Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 FIZZULTXMVEIAA-IHRRRGAJSA-N 0.000 claims 1
- MDSUKZSLOATHMH-UHFFFAOYSA-N N-L-leucyl-L-valine Natural products CC(C)CC(N)C(=O)NC(C(C)C)C(O)=O MDSUKZSLOATHMH-UHFFFAOYSA-N 0.000 claims 1
- GZFAWAQTEYDKII-YUMQZZPRSA-N Ser-Gly-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)CO GZFAWAQTEYDKII-YUMQZZPRSA-N 0.000 claims 1
- NNFMANHDYSVNIO-DCAQKATOSA-N Ser-Lys-Arg Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O NNFMANHDYSVNIO-DCAQKATOSA-N 0.000 claims 1
- IRLYZKKNBFPQBW-XGEHTFHBSA-N Val-Cys-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CS)NC(=O)[C@H](C(C)C)N)O IRLYZKKNBFPQBW-XGEHTFHBSA-N 0.000 claims 1
- 108010091798 leucylleucine Proteins 0.000 claims 1
- 108010018625 phenylalanylarginine Proteins 0.000 claims 1
- 108010061238 threonyl-glycine Proteins 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 82
- 108020004999 messenger RNA Proteins 0.000 description 59
- 210000004027 cell Anatomy 0.000 description 53
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 29
- 239000008103 glucose Substances 0.000 description 29
- 239000000523 sample Substances 0.000 description 29
- 235000001727 glucose Nutrition 0.000 description 28
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 27
- 229940088598 enzyme Drugs 0.000 description 23
- 108091092195 Intron Proteins 0.000 description 21
- 239000002773 nucleotide Substances 0.000 description 21
- 125000003729 nucleotide group Chemical group 0.000 description 21
- 239000000047 product Substances 0.000 description 18
- 239000006228 supernatant Substances 0.000 description 18
- 241000283973 Oryctolagus cuniculus Species 0.000 description 17
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 16
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- 101100462537 Caenorhabditis elegans pac-1 gene Proteins 0.000 description 15
- 101100117764 Mus musculus Dusp2 gene Proteins 0.000 description 15
- YQNRVGJCPCNMKT-LFVJCYFKSA-N 2-[(e)-[[2-(4-benzylpiperazin-1-ium-1-yl)acetyl]hydrazinylidene]methyl]-6-prop-2-enylphenolate Chemical compound [O-]C1=C(CC=C)C=CC=C1\C=N\NC(=O)C[NH+]1CCN(CC=2C=CC=CC=2)CC1 YQNRVGJCPCNMKT-LFVJCYFKSA-N 0.000 description 14
- 230000010076 replication Effects 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 13
- 210000001938 protoplast Anatomy 0.000 description 13
- 239000006166 lysate Substances 0.000 description 12
- 244000005700 microbiome Species 0.000 description 12
- 108090000765 processed proteins & peptides Proteins 0.000 description 12
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 11
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 11
- 125000003275 alpha amino acid group Chemical group 0.000 description 11
- 239000000499 gel Substances 0.000 description 11
- 101710194570 Glucoamylase I Proteins 0.000 description 10
- 102000004196 processed proteins & peptides Human genes 0.000 description 10
- 235000002639 sodium chloride Nutrition 0.000 description 10
- 238000013519 translation Methods 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- 238000005119 centrifugation Methods 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 239000012528 membrane Substances 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 239000011780 sodium chloride Substances 0.000 description 9
- 238000003786 synthesis reaction Methods 0.000 description 9
- 238000013518 transcription Methods 0.000 description 9
- 230000035897 transcription Effects 0.000 description 9
- 108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 8
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 8
- 238000003556 assay Methods 0.000 description 8
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 8
- 238000001502 gel electrophoresis Methods 0.000 description 8
- 230000012010 growth Effects 0.000 description 8
- 229920001184 polypeptide Polymers 0.000 description 8
- 238000011160 research Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 241000228212 Aspergillus Species 0.000 description 7
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 7
- 102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 7
- 230000015556 catabolic process Effects 0.000 description 7
- 238000006731 degradation reaction Methods 0.000 description 7
- 238000009396 hybridization Methods 0.000 description 7
- 108091008146 restriction endonucleases Proteins 0.000 description 7
- 238000012163 sequencing technique Methods 0.000 description 7
- 239000013595 supernatant sample Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 102000004594 DNA Polymerase I Human genes 0.000 description 6
- 108010017826 DNA Polymerase I Proteins 0.000 description 6
- 108091034117 Oligonucleotide Proteins 0.000 description 6
- 108091000080 Phosphotransferase Proteins 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000008188 pellet Substances 0.000 description 6
- 102000020233 phosphotransferase Human genes 0.000 description 6
- 239000011541 reaction mixture Substances 0.000 description 6
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 5
- 239000007983 Tris buffer Substances 0.000 description 5
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 230000003834 intracellular effect Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 230000035772 mutation Effects 0.000 description 5
- 210000001995 reticulocyte Anatomy 0.000 description 5
- 239000001509 sodium citrate Substances 0.000 description 5
- 108020004705 Codon Proteins 0.000 description 4
- 102100031780 Endonuclease Human genes 0.000 description 4
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
- 150000001720 carbohydrates Chemical class 0.000 description 4
- 235000014633 carbohydrates Nutrition 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000010276 construction Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 229940027941 immunoglobulin g Drugs 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 239000006151 minimal media Substances 0.000 description 4
- 238000002741 site-directed mutagenesis Methods 0.000 description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 108010039636 3-isopropylmalate dehydrogenase Proteins 0.000 description 3
- 108010065511 Amylases Proteins 0.000 description 3
- 102000013142 Amylases Human genes 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 3
- 239000000020 Nitrocellulose Substances 0.000 description 3
- 108700026244 Open Reading Frames Proteins 0.000 description 3
- 241000364057 Peoria Species 0.000 description 3
- 108020004511 Recombinant DNA Proteins 0.000 description 3
- 244000206963 Saccharomyces cerevisiae var. diastaticus Species 0.000 description 3
- 108020004682 Single-Stranded DNA Proteins 0.000 description 3
- 108091081024 Start codon Proteins 0.000 description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 description 3
- 235000020054 awamori Nutrition 0.000 description 3
- 150000001540 azides Chemical class 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 3
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- 238000001114 immunoprecipitation Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 229920001220 nitrocellulos Polymers 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 230000008488 polyadenylation Effects 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 230000002194 synthesizing effect Effects 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 2
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 241000235349 Ascomycota Species 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- 229920002271 DEAE-Sepharose Polymers 0.000 description 2
- 101710088194 Dehydrogenase Proteins 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 102000004533 Endonucleases Human genes 0.000 description 2
- 108060002716 Exonuclease Proteins 0.000 description 2
- 229920001917 Ficoll Polymers 0.000 description 2
- AYRXSINWFIIFAE-SCLMCMATSA-N Isomaltose Natural products OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@@H](O)[C@@H](O)[C@@H]1O AYRXSINWFIIFAE-SCLMCMATSA-N 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- 102000003960 Ligases Human genes 0.000 description 2
- 108090000364 Ligases Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 239000003391 RNA probe Substances 0.000 description 2
- 235000001006 Saccharomyces cerevisiae var diastaticus Nutrition 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- ZMZDMBWJUHKJPS-UHFFFAOYSA-M Thiocyanate anion Chemical compound [S-]C#N ZMZDMBWJUHKJPS-UHFFFAOYSA-M 0.000 description 2
- 102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 2
- 108700015934 Triose-phosphate isomerases Proteins 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 229960005305 adenosine Drugs 0.000 description 2
- 108090000637 alpha-Amylases Proteins 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 239000005546 dideoxynucleotide Substances 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 150000002085 enols Chemical class 0.000 description 2
- 102000013165 exonuclease Human genes 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- ZMZDMBWJUHKJPS-UHFFFAOYSA-N hydrogen thiocyanate Natural products SC#N ZMZDMBWJUHKJPS-UHFFFAOYSA-N 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000013600 plasmid vector Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000037452 priming Effects 0.000 description 2
- 238000000163 radioactive labelling Methods 0.000 description 2
- 239000012857 radioactive material Substances 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 108020004418 ribosomal RNA Proteins 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 230000005026 transcription initiation Effects 0.000 description 2
- 230000005030 transcription termination Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000001226 triphosphate Substances 0.000 description 2
- 235000011178 triphosphate Nutrition 0.000 description 2
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 description 1
- 108010043797 4-alpha-glucanotransferase Proteins 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
- 101710187578 Alcohol dehydrogenase 1 Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 101000757144 Aspergillus niger Glucoamylase Proteins 0.000 description 1
- CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 230000007023 DNA restriction-modification system Effects 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 108050008938 Glucoamylases Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 101150017040 I gene Proteins 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 101150007280 LEU2 gene Proteins 0.000 description 1
- 102000008072 Lymphokines Human genes 0.000 description 1
- 108010074338 Lymphokines Proteins 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
- 102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102000009572 RNA Polymerase II Human genes 0.000 description 1
- 108010009460 RNA Polymerase II Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241001123227 Saccharomyces pastorianus Species 0.000 description 1
- 241000582914 Saccharomyces uvarum Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000013602 bacteriophage vector Substances 0.000 description 1
- 238000007630 basic procedure Methods 0.000 description 1
- 108010019077 beta-Amylase Proteins 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000012847 fine chemical Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 238000004362 fungal culture Methods 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 235000021472 generally recognized as safe Nutrition 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 150000002304 glucoses Chemical class 0.000 description 1
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- AWUCVROLDVIAJX-UHFFFAOYSA-N glycerol 1-phosphate Chemical compound OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 229940047124 interferons Drugs 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 101150019727 malQ gene Proteins 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 150000002972 pentoses Chemical group 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920006316 polyvinylpyrrolidine Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 239000013605 shuttle vector Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 238000005549 size reduction Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 108010082737 zymolyase Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US46192083A | 1983-01-28 | 1983-01-28 | |
US56407883A | 1983-12-20 | 1983-12-20 | |
US56394183A | 1983-12-20 | 1983-12-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
NO840200L true NO840200L (no) | 1984-07-30 |
Family
ID=27412854
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO840200A NO840200L (no) | 1983-01-28 | 1984-01-19 | Glukoamylase cdna. |
Country Status (10)
Country | Link |
---|---|
EP (2) | EP0126206B1 (fr) |
AU (1) | AU567031B2 (fr) |
DE (3) | DE3485723D1 (fr) |
DK (1) | DK36384A (fr) |
FI (1) | FI840295A (fr) |
IE (1) | IE840214L (fr) |
IL (1) | IL70811A (fr) |
NO (1) | NO840200L (fr) |
NZ (1) | NZ207000A (fr) |
WO (1) | WO1984002921A2 (fr) |
Families Citing this family (194)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5422267A (en) * | 1984-05-22 | 1995-06-06 | Robert R. Yocum | Industrial yeast comprising an integrated glucoamylase gene |
US4879231A (en) * | 1984-10-30 | 1989-11-07 | Phillips Petroleum Company | Transformation of yeasts of the genus pichia |
JPS61141890A (ja) * | 1984-12-15 | 1986-06-28 | Suntory Ltd | アルコ−ルの製造法 |
JPH0630586B2 (ja) * | 1984-12-15 | 1994-04-27 | サントリー株式会社 | グルコアミラ−ゼ遺伝子 |
GB8432483D0 (en) * | 1984-12-21 | 1985-02-06 | Tubb R S | Enzymes |
GB8508800D0 (en) * | 1985-04-04 | 1985-05-09 | Glaxo Group Ltd | Microbiological process |
US5198345A (en) * | 1985-04-15 | 1993-03-30 | Gist-Brocades N.V. | Vectors in use in filamentous fungi |
DE3650783T2 (de) * | 1985-04-15 | 2004-07-15 | Dsm Ip Assets B.V. | Verwendung des Glucoamylasepromotors aus Apergillus |
US5821079A (en) * | 1985-05-02 | 1998-10-13 | Transgene S.A. | Vectors for the expression and secretion of hirudin by transformed yeasts |
WO1986007091A1 (fr) * | 1985-05-21 | 1986-12-04 | Biotechnica International, Inc. | Levure exprimant de la glucoamylase |
AU607398B2 (en) * | 1985-08-29 | 1991-03-07 | Genencor Inc. | Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same |
US6004785A (en) * | 1985-08-29 | 1999-12-21 | Genencor International Inc. | Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same |
US5364770A (en) * | 1985-08-29 | 1994-11-15 | Genencor International Inc. | Heterologous polypeptides expressed in aspergillus |
US5024941A (en) * | 1985-12-18 | 1991-06-18 | Biotechnica International, Inc. | Expression and secretion vector for yeast containing a glucoamylase signal sequence |
US5536661A (en) * | 1987-03-10 | 1996-07-16 | Novo Nordisk A/S | Process for the production of protein products in aspergillus |
US5766912A (en) * | 1986-03-17 | 1998-06-16 | Novo Nordisk A/S | Humicola lipase produced in aspergillus |
FR2613727B2 (fr) * | 1986-06-10 | 1990-07-20 | Transgene Sa | Integration dans la souche polyploide saccharomyces uvarum 2285 tg2 amgc du bloc d'expression de l'alpha-amylase de b. licheniformis |
FR2613726B2 (fr) * | 1986-06-10 | 1990-07-20 | Transgene Sa | Integration dans la souche polyploide de boulangerie (tgf1) du bloc d'expression de l'alpha-amyloglucosidase de aspergillus niger |
FR2599755B1 (fr) * | 1986-06-10 | 1990-04-20 | Transgene Sa | Bloc d'expression d'une amyloglucosidase dans une levure, levure transformee et procede de preparation d'enzyme |
DK293787A (da) * | 1986-06-10 | 1987-12-11 | Transgene Sa | Ekspression af en amyloglucosidase |
MC1834A1 (fr) * | 1986-07-10 | 1988-06-03 | Transgene Sa | Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation |
EP0260404B1 (fr) * | 1986-08-21 | 1995-05-31 | VAN DEN BERG, Robert | Microorganismes produisant des enzymes amylolytiques construits par la technologie des ADN recombinants et leur utilisation dans des procédés de fermentation |
US5151354A (en) * | 1986-08-21 | 1992-09-29 | Cornelius Hollenberg | Fermentation processes using amylolytic enzyme producing microorganisms |
EP0257115A1 (fr) * | 1986-08-21 | 1988-03-02 | Heineken Technisch Beheer B.V. | Micro-organismes produisant des enzymes amylolytiques et construits par la technologie des ADN recombinants et leur utilisation dans des procédés de fermentation |
GB8621118D0 (en) * | 1986-09-01 | 1986-10-08 | Whitbread & Co Plc | Stabilised polypeptides |
US5013652A (en) * | 1986-10-14 | 1991-05-07 | Genex Corporation | Composite yeast vectors |
FI92333C (fi) * | 1987-03-17 | 1994-10-25 | Panimolaboratorio Bryggerilabo | -asetolaktaattidekarboksylaasiaktiivisuutta omaavaa entsyymiä koodaava DNA-sekvenssi ja sen käyttö nopeutettuun oluen valmistukseen soveltuvien hiivakantojen rakentamiseksi |
US5665585A (en) * | 1992-09-03 | 1997-09-09 | Alko-Yhiot Oy | Recombinant production of glucoamylase P in trichoderma |
BE1008737A3 (fr) * | 1994-01-28 | 1996-07-02 | Solvay | Systeme d'expression, vecteur d'integration et cellule transformee par ce vecteur d'integration. |
BE1008738A3 (fr) * | 1994-06-17 | 1996-07-02 | Solvay | Systeme d'expression, vecteur d'integration et cellule transformee par ce vecteur d'integration. |
JPH0875585A (ja) * | 1994-09-09 | 1996-03-22 | Smc Corp | 圧力検出器の接続構造 |
US6255084B1 (en) * | 1997-11-26 | 2001-07-03 | Novozymes A/S | Thermostable glucoamylase |
US6352851B1 (en) | 1998-07-15 | 2002-03-05 | Novozymes A/S | Glucoamylase variants |
KR20010089885A (ko) * | 1998-12-07 | 2001-10-12 | 피아 스타르 | N-말단 연장을 가진 글루코아밀라제 |
US6329186B1 (en) | 1998-12-07 | 2001-12-11 | Novozymes A/S | Glucoamylases with N-terminal extensions |
AU5061000A (en) * | 1999-06-02 | 2000-12-28 | Novozymes A/S | Novel glucoamylase |
EP1434861A2 (fr) | 2001-10-01 | 2004-07-07 | Novozymes A/S | Variants de glucoamylase |
US20040063184A1 (en) | 2002-09-26 | 2004-04-01 | Novozymes North America, Inc. | Fermentation processes and compositions |
CN1788083B (zh) | 2003-03-10 | 2011-10-05 | 诺维信公司 | 酒精产品生产方法 |
CN100506997C (zh) | 2003-05-30 | 2009-07-01 | 诺维信公司 | 酒精产品生产方法 |
US20040253696A1 (en) | 2003-06-10 | 2004-12-16 | Novozymes North America, Inc. | Fermentation processes and compositions |
WO2004113551A1 (fr) | 2003-06-25 | 2004-12-29 | Novozymes A/S | Procede d'hydrolyse de l'amidon |
MXPA06004463A (es) | 2003-10-28 | 2006-06-27 | Novozymes North America Inc | Enzimas hibridas. |
US7332319B2 (en) | 2004-05-27 | 2008-02-19 | Genencor International, Inc. | Heterologous alpha amylase expression in Aspergillus |
WO2005117756A2 (fr) | 2004-05-27 | 2005-12-15 | Genencor International, Inc. | Alpha-amylases stables en milieu acide presentant une activite d'hydrolyse de l'amidon granulaire et compositions d'enzymes |
US8841091B2 (en) | 2004-12-22 | 2014-09-23 | Novozymes Als | Enzymes for starch processing |
WO2006069289A2 (fr) * | 2004-12-22 | 2006-06-29 | Novozymes North America, Inc | Polypeptides presentant l'activite d'une glucoamylase, et polynucleotides encodant ces polypeptides |
JP5087407B2 (ja) | 2004-12-30 | 2012-12-05 | ジェネンコー・インターナショナル・インク | 酸性真菌プロテアーゼ |
EP1941049A4 (fr) | 2005-09-20 | 2011-12-21 | Novozymes North America Inc | Procédé de production d'un produit de fermentation |
US20080318284A1 (en) | 2005-12-22 | 2008-12-25 | Novozymes North America, Inc. | Processes for Producing a Fermentation Product |
CN101405397A (zh) | 2006-03-22 | 2009-04-08 | 诺维信北美公司 | 发酵方法 |
US7871800B2 (en) | 2006-04-19 | 2011-01-18 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
US7968318B2 (en) | 2006-06-06 | 2011-06-28 | Genencor International, Inc. | Process for conversion of granular starch to ethanol |
CN101679987A (zh) | 2007-03-09 | 2010-03-24 | 丹尼斯科美国公司 | 嗜碱芽孢杆菌物种α-淀粉酶变体、包括α-淀粉酶变体的组合物以及使用方法 |
CN101636490B (zh) | 2007-03-14 | 2012-05-16 | 丹尼斯科美国公司 | 里氏木霉α-淀粉酶是一种生麦芽糖酶 |
CA2681328C (fr) | 2007-03-14 | 2015-10-06 | Danisco Us Inc. | Production d'ethanol a partir d'orge et de ddgs contenant des niveaux reduits de beta-glucane et d'acide phytique |
EP3219804B1 (fr) | 2007-04-24 | 2019-07-17 | Novozymes North America, Inc. | Detoxification de matériaux contenant de la lignocellulose prétraitée |
WO2009030713A1 (fr) | 2007-09-03 | 2009-03-12 | Novozymes A/S | Détoxification et recyclage de solutions de lavage utilisées dans le prétraitement de matériaux contenant de la lignocellulose |
WO2009100102A2 (fr) | 2008-02-04 | 2009-08-13 | Danisco Us Inc., Genencor Division | Variants ts23 de l’alpha-amylase à propriétés modifiées |
US8354256B2 (en) | 2008-03-11 | 2013-01-15 | Danisco Us Inc. | Glucoamylase and Buttiauxiella phytase during saccharification |
CN101970672A (zh) | 2008-03-11 | 2011-02-09 | 丹尼斯科美国公司 | 根霉属淀粉酶在颗粒淀粉水解中的应用 |
MX2010011721A (es) | 2008-04-30 | 2010-11-30 | Danisco Inc | Nuevas variantes de alfa-amilasa quimericas. |
US9040278B2 (en) | 2008-06-06 | 2015-05-26 | Danisco Us Inc. | Production of glucose from starch using alpha-amylases from Bacillus subtilis |
EP2291526B1 (fr) | 2008-06-06 | 2014-08-13 | Danisco US Inc. | Composition d'enzymes de saccharification comprenant une alpha-amylase de Bacillus subtilis |
DK2297312T3 (da) | 2008-06-06 | 2013-12-16 | Danisco Us Inc | Alpha-amylasevarianter af Bacillus subtilis og fremgangsmåder til anvendelse heraf |
CA2726688A1 (fr) | 2008-06-23 | 2010-01-21 | Novozymes A/S | Procedes de production de produits de fermentation |
CN102171350B (zh) | 2008-09-30 | 2013-09-11 | 诺维信北美公司 | 对用干酒糟预处理含木素纤维素材料的酶水解的改进 |
WO2010043538A2 (fr) | 2008-10-15 | 2010-04-22 | Novozymes A/S | Procédé de brassage |
WO2010059413A2 (fr) | 2008-11-20 | 2010-05-27 | Novozymes, Inc. | Polypeptides ayant une activité amylolytique renforcée et polynucléotides codant pour ceux-ci |
WO2010078391A2 (fr) | 2008-12-30 | 2010-07-08 | Novozymes North America, Inc. | Amélioration d'une hydrolyse enzymatique d'une matière prétraitée contenant de la lignocellulose avec de la boue d'aéroflottation dissoute |
WO2010078392A2 (fr) | 2008-12-31 | 2010-07-08 | Novozymes North America, Inc. | Procédés de production de produits de fermentation |
BRPI1010238A2 (pt) | 2009-04-01 | 2015-08-25 | Danisco Us Inc | Composições e métodos que comprendem variantes de alfa-amilase com propriedades alteradas |
US20120088285A1 (en) | 2009-07-07 | 2012-04-12 | Novozymes A/S | Process For Treating A Substrate With An Enzyme |
CN102498401B (zh) | 2009-07-17 | 2015-12-02 | 诺维信公司 | 在纤维素材料水解中分析纤维素衰变的方法 |
CA2771071C (fr) | 2009-08-07 | 2020-03-10 | Danisco Us Inc. | Melange d'alpha-amylases pour le traitement d'un amidon et procede pour l'utiliser |
CN102665425A (zh) | 2009-09-30 | 2012-09-12 | 诺维信公司 | 馒头制备方法和馒头改进组合物 |
EP2491121A2 (fr) | 2009-10-23 | 2012-08-29 | Danisco US Inc. | Procédés destinés à réduire le saccharide donnant une couleur bleue |
US20120252086A1 (en) | 2009-12-22 | 2012-10-04 | Novozymes A/S | Compositions Comprising Boosting Polypeptide And Starch Degrading Enzyme And Uses Thereof |
WO2011092136A1 (fr) | 2010-01-29 | 2011-08-04 | Novozymes A/S | Procédé de production de biogaz avec prétraitement enzymatique |
WO2011100161A1 (fr) | 2010-02-09 | 2011-08-18 | Novozymes North America, Inc. | Addition d'alpha-glucosidase et de cobalt pour préparer des produits de fermentation à partir d'amidon |
EP3070171B1 (fr) | 2010-03-30 | 2018-06-13 | Novozymes A/S | Procede pour augmenter les produits secondaires de procedes de fermentation |
DK2558484T3 (en) | 2010-04-14 | 2016-03-29 | Novozymes As | Polypeptides having glucoamylase activity and polynucleotides encoding them |
EP2579727B1 (fr) | 2010-06-11 | 2018-08-08 | Novozymes A/S | Correction enzymatique de la farine |
CN103140586A (zh) | 2010-08-02 | 2013-06-05 | 诺维信北美公司 | 产生发酵产物的方法 |
CA2813134A1 (fr) | 2010-10-13 | 2012-04-19 | Novozymes A/S | Preparation d'un produit cuit a partir de pate |
CA2817224A1 (fr) | 2010-11-19 | 2012-05-24 | Novozymes North America, Inc. | Procede de production d'un produit de fermentation |
ES2673940T3 (es) | 2010-12-22 | 2018-06-26 | Novozymes North America, Inc. | Proceso para producir productos de fermentación a partir de materiales que contienen almidón |
EP2661499A1 (fr) | 2011-01-04 | 2013-11-13 | Novozymes A/S | Procédé de production d'un gaz biologique à partir d'un matériau contenant de la pectine et de la lignocellulose |
US9677094B2 (en) | 2011-02-07 | 2017-06-13 | Novozymes A/S | Process of producing a fermentation product |
EP2702161A1 (fr) | 2011-04-29 | 2014-03-05 | Danisco US Inc. | Utilisation de cellulase et de glycoamylase pour améliorer des rendements d'éthanol provenant de fermentation |
BR112013032543A2 (pt) | 2011-06-28 | 2017-01-17 | Novozymes As | processo de produção de biogás |
EP2729572B1 (fr) | 2011-07-06 | 2019-03-20 | Novozymes A/S | Variants d'alpha-amylase et polynucléotides codant ces variants |
EP2787070A3 (fr) | 2011-07-21 | 2015-03-11 | AB Enzymes GmbH | Procédé pour lyser des parois cellulaires de levure |
CN109022518A (zh) | 2011-07-22 | 2018-12-18 | 诺维信北美公司 | 用于预处理纤维素材料和改进其水解的方法 |
CA2849401A1 (fr) | 2011-09-29 | 2013-04-04 | Danisco Us Inc. | Liquefaction et saccharification d'amidon granulaire a une concentration elevee |
WO2013055676A1 (fr) | 2011-10-11 | 2013-04-18 | Novozymes North America, Inc. | Procédés de production de produits de fermentation |
AU2012328562A1 (en) | 2011-10-28 | 2014-03-13 | Danisco Us Inc. | Variant maltohexaose-forming alpha-amylase variants |
IN2014CN04905A (fr) | 2011-12-02 | 2015-09-18 | Novozymes As | |
WO2013083801A2 (fr) | 2011-12-09 | 2013-06-13 | Novozymes A/S | Biogaz obtenu à partir de substrats comprenant du fumier de ferme et des enzymes |
US20150017670A1 (en) | 2011-12-21 | 2015-01-15 | Novozymes, Inc. | Methods For Determining The Degradation Of A Biomass Material |
EP4209595A1 (fr) | 2012-03-30 | 2023-07-12 | Novozymes North America, Inc. | Procédé de déshydratation de résidus de distillation entiers |
ES2935920T3 (es) | 2012-03-30 | 2023-03-13 | Novozymes North America Inc | Procesos de elaboración de productos de fermentación |
WO2013160370A1 (fr) | 2012-04-25 | 2013-10-31 | Novozymes A/S | Procédé de cuisson |
MX2014013402A (es) | 2012-05-11 | 2014-11-26 | Danisco Inc | Uso de alfa-amilasa de aspergillus clavatus para sacarificacion. |
DK2825643T3 (da) | 2012-06-08 | 2021-11-08 | Danisco Us Inc | Variant-alfa-amylaser med forbedret aktivitet over for stivelsespolymerer |
WO2014001351A1 (fr) | 2012-06-27 | 2014-01-03 | Novozymes A/S | Procédé de cuisson de riz |
EP2870256A1 (fr) | 2012-07-06 | 2015-05-13 | Novozymes A/S | Inactivation d'une souche de production au moyen d'un acide gras |
WO2014028434A2 (fr) | 2012-08-16 | 2014-02-20 | Danisco Us Inc. | Procédé d'utilisation d'alpha-amylase provenant d'aspergillus clavatus et de pullulanase en vue d'une saccharification |
BR112015003257A2 (pt) | 2012-08-17 | 2017-11-14 | Novozymes As | variante de asparaginase, métodos para produção de um produto alimentar a partir de um material alimentar, para produção de uma variante de asparaginase e para obtenção de uma variante de asparaginase, polinucleotídeo isolado, constructo de ácido nucleico, vetor de expressão, e, célula hospedeira |
WO2014081622A1 (fr) | 2012-11-20 | 2014-05-30 | Danisco Us Inc. | Amylase ayant des propriétés maltogéniques |
CA2894513A1 (fr) | 2012-12-14 | 2014-06-19 | Danisco Us Inc. | Procede d'utilisation d'alpha-amylase provenant d'aspergillus fumigatus et d'isoamylase pour une saccharification |
US20160010128A1 (en) | 2012-12-20 | 2016-01-14 | Danisco Us Inc. | Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification |
WO2014099525A1 (fr) | 2012-12-21 | 2014-06-26 | Danisco Us Inc. | Amylase de paenibacillus curdlanolyticus, et ses procédés d'utilisation |
WO2014099523A1 (fr) | 2012-12-21 | 2014-06-26 | Danisco Us Inc. | Variants d'alpha-amylase |
ES2676895T5 (es) | 2013-03-11 | 2022-04-27 | Danisco Us Inc | Variantes combinatorias de alfa-amilasa |
WO2014200657A1 (fr) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase provenant destreptomyces xiamenensis |
WO2014200656A1 (fr) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase provenant de streptomyces umbrinus |
WO2014200658A1 (fr) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase issue de promicromonospora vindobonensis |
EP3011020A1 (fr) | 2013-06-17 | 2016-04-27 | Danisco US Inc. | Alpha-amylase issue d'un membre de la famille des bacillaceae |
CA2915336C (fr) | 2013-07-17 | 2024-03-12 | Novozymes A/S | Chimeres de pullulanase et polynucleotides les codant |
US9951364B2 (en) | 2013-09-11 | 2018-04-24 | Novozymes A/S | Processes for producing fermentation products |
WO2015050723A1 (fr) | 2013-10-03 | 2015-04-09 | Danisco Us Inc. | Alpha-amylases provenant de exiguobacterium, méthodes d'utilisation de celles-ci |
EP3052622B1 (fr) | 2013-10-03 | 2018-09-19 | Danisco US Inc. | Alpha-amylases faisant partie d'un sous-ensemble d'exiguobacterium, et procédés d'utilisation correspondants |
WO2015057517A1 (fr) | 2013-10-17 | 2015-04-23 | Danisco Us Inc. | Utilisation d'hémicellulases pour améliorer la production d'éthanol |
BR112016009365A2 (pt) | 2013-10-28 | 2017-09-19 | Danisco Us Inc | Método para aumentar, melhorar e estender a produção de etanol e método para diminuir as concentrações finais de dp2 em um produto de fermentação |
WO2015066667A1 (fr) | 2013-11-04 | 2015-05-07 | Danisco Us Inc. | Protéases utilisées dans le traitement du blé |
WO2015066669A1 (fr) | 2013-11-04 | 2015-05-07 | Danisco Us Inc. | Protéases utilisables dans le cadre du traitement du maïs |
MX2016006489A (es) | 2013-11-20 | 2016-08-03 | Danisco Us Inc | Alfa-amilasas variantes que tienen susceptibilidad reducida a la escision por proteasas y metodos de uso. |
WO2015094809A1 (fr) | 2013-12-19 | 2015-06-25 | Danisco Us Inc. | Alpha-amylases chimères fongiques comprenant un module de liaison aux glucides et utilisation de celles-ci |
WO2015094714A1 (fr) | 2013-12-19 | 2015-06-25 | Danisco Us Inc. | Protéases dans le traitement des grains |
DK3097192T3 (en) | 2014-01-22 | 2018-11-19 | Novozymes As | PULLULANASE VARIATIONS AND POLYNUCLEOTIDES CODING THEM |
RU2019134734A (ru) | 2014-02-07 | 2019-11-18 | Новозимс А/С | Композиции для получения глюкозных сиропов |
WO2015143144A1 (fr) | 2014-03-19 | 2015-09-24 | Novozymes A/S | Procédé destiné à augmenter l'activité d'un polypeptide x143 |
EP3129478B1 (fr) | 2014-04-10 | 2019-03-27 | Novozymes A/S | Variants d'alpha-amylase et polynucléotides codant pour ces derniers |
US10584324B2 (en) | 2014-10-23 | 2020-03-10 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2016087445A1 (fr) | 2014-12-01 | 2016-06-09 | Novozymes A/S | Production améliorée de sirops de glucose |
CN108699578A (zh) | 2015-03-11 | 2018-10-23 | 杰能科国际有限公司 | 裂解性多糖单加氧酶的酶活性 |
CN108350442A (zh) | 2015-06-18 | 2018-07-31 | 诺维信公司 | 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途 |
DK3313999T3 (da) | 2015-06-24 | 2021-08-02 | Genencor Int Bv | Polypeptider med demethyleringsaktivitet |
WO2017100720A1 (fr) | 2015-12-09 | 2017-06-15 | Danisco Us Inc. | Variants combinatoires d'alpha-amylases |
WO2017112539A1 (fr) | 2015-12-22 | 2017-06-29 | Novozymes A/S | Procédé d'extraction d'huile de résidu de distillation soluble |
US20190029272A1 (en) | 2016-02-10 | 2019-01-31 | Novozymes A/S | Preparation of a Baked Product Comprising Fibers Treated by a Cellulase |
WO2017173324A2 (fr) | 2016-04-01 | 2017-10-05 | Danisco Us Inc. | Alpha-amylases, compositions et procédés |
WO2017173190A2 (fr) | 2016-04-01 | 2017-10-05 | Danisco Us Inc. | Alpha-amylases, compositions et procédés |
CA3027464A1 (fr) | 2016-07-15 | 2018-01-18 | Novozymes A/S | Amelioration de l'aptitude de pains plats a etre roules |
RU2763378C2 (ru) | 2016-09-23 | 2021-12-28 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | ПРИМЕНЕНИЕ АКТИВНЫХ ПРИ НИЗКОМ ЗНАЧЕНИИ pH АЛЬФА-1,4/1,6-ГЛИКОЗИДГИДРОЛАЗ В КАЧЕСТВЕ КОРМОВОЙ ДОБАВКИ ДЛЯ ЖВАЧНЫХ ЖИВОТНЫХ ДЛЯ УЛУЧШЕНИЯ ПЕРЕВАРИВАНИЯ КРАХМАЛА |
US11015212B2 (en) | 2016-10-17 | 2021-05-25 | Novozymes A/S | Methods of reducing foam during ethanol fermentation |
WO2018098381A1 (fr) | 2016-11-23 | 2018-05-31 | Novozymes A/S | Levure améliorée pour la production d'éthanol |
MX2019006122A (es) | 2016-11-30 | 2019-08-14 | Novozymes As | Metodo de hornear. |
MX2019009551A (es) | 2017-02-20 | 2020-01-30 | Novozymes As | Enzima lipolitica para usarse en horneado. |
WO2018184004A1 (fr) | 2017-03-31 | 2018-10-04 | Danisco Us Inc | Variants combinatoires d'alpha-amylases |
BR112019025391A2 (pt) | 2017-06-02 | 2020-07-07 | Novozymes A/S | levedura melhorada para a produção de etanol |
WO2018226569A1 (fr) | 2017-06-06 | 2018-12-13 | Danisco Us Inc | Utilisation de la bétaïne pour stabiliser et/ou augmenter l'activité d'enzymes dans des environnements stressants |
WO2018234382A1 (fr) | 2017-06-22 | 2018-12-27 | Novozymes A/S | Relaxation d'une pâte à l'aide d'une gamma glutamyle transpeptidase |
EP4015524A1 (fr) | 2017-06-28 | 2022-06-22 | Novozymes A/S | Polypeptides présentant une activité tréhalase et polynucléotides codant pour ceux-ci |
US11220679B2 (en) | 2017-08-08 | 2022-01-11 | Novozymes A/S | Polypeptides having trehalase activity |
WO2019042971A1 (fr) | 2017-08-29 | 2019-03-07 | Novozymes A/S | Levure de boulangerie exprimant des amylases anti-rassissement/fraîcheur |
CA3070730A1 (fr) | 2017-09-15 | 2019-03-21 | Novozymes A/S | Melanges d'enzymes et procedes pour ameliorer la qualite nutritionnelle d'aliments pour animaux |
EP3701037A1 (fr) | 2017-10-23 | 2020-09-02 | Novozymes A/S | Procédés pour la réduction d'acide lactique dans un système de fermentation de biocarburant |
BR112020009525A2 (pt) | 2017-11-14 | 2020-11-03 | Danisco Us Inc. | alfa-amilase, composição e método |
CN113286871A (zh) | 2018-01-29 | 2021-08-20 | 诺维信公司 | 用于乙醇生产的氮利用提高的微生物 |
AU2019222480A1 (en) | 2018-02-15 | 2020-10-08 | Microbiogen Pty. Ltd. | Improved yeast for ethanol production |
WO2019201725A1 (fr) | 2018-04-19 | 2019-10-24 | Novozymes A/S | Procédé pour améliorer la fraîcheur de pains plats impliquant une combinaison de variants d'alpha-amylase maltogénique |
WO2019231944A2 (fr) | 2018-05-31 | 2019-12-05 | Novozymes A/S | Procédés d'amélioration de la croissance et de la productivité de levures |
CA3101914A1 (fr) | 2018-06-04 | 2019-12-12 | Novozymes A/S | Article enzymatique solide destine a etre utilise dans la cuisson |
EP3806642A1 (fr) | 2018-06-12 | 2021-04-21 | Novozymes A/S | Quantité réduite de sucre ajouté dans des produits cuits |
BR112021000369A2 (pt) | 2018-07-11 | 2021-04-13 | Novozymes A/S | Processos para produção de produtos de fermentação |
CN112601818A (zh) | 2018-07-25 | 2021-04-02 | 诺维信公司 | 用于生产乙醇的表达酶的酵母 |
US11807889B2 (en) | 2018-10-08 | 2023-11-07 | Novozymes A/S | Yeast expressing a heterologous phospholipase for ethanol production |
BR112021014873A2 (pt) | 2019-01-31 | 2021-10-05 | Novozymes A/S | Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação |
US20220159975A1 (en) | 2019-03-13 | 2022-05-26 | Novozymes A/S | Production of Par-Baked Products with Improved Freshness Employing Combination of GH8 Xylanase and Phospholipase |
BR112021017085A2 (pt) | 2019-04-02 | 2022-02-08 | Novozymes As | Processo de produção de um produto de fermentação |
AU2020310162A1 (en) | 2019-07-09 | 2022-02-03 | International N&H Denmark Aps | Fat coated particulate enzyme compositions |
CN114450390A (zh) | 2019-07-26 | 2022-05-06 | 诺维信公司 | 用于乙醇生产的氮转运提高的微生物 |
CA3144423A1 (fr) | 2019-08-05 | 2021-02-11 | Kurt Creamer | Melanges d'enzymes et procedes de production d'un ingredient d'alimentation animale a haute teneur en proteines a partir d'un sous-produit de type residu de distillation entier |
WO2021025872A1 (fr) | 2019-08-06 | 2021-02-11 | Novozymes A/S | Protéines de fusion pour une expression enzymatique améliorée |
WO2021046073A1 (fr) | 2019-09-05 | 2021-03-11 | Dupont Nutrition Biosciences Aps | Composition d'aliment pour animaux |
MX2022002834A (es) | 2019-09-16 | 2022-04-06 | Novozymes As | Polipeptidos con actividad beta-glucanasa y polinucleotidos que los codifican. |
WO2021089750A1 (fr) | 2019-11-08 | 2021-05-14 | Novozymes A/S | Compositions enzymatiques liquides stabilisées pour brassage |
CN114929022A (zh) | 2019-12-09 | 2022-08-19 | 诺维信公司 | 烘焙添加剂 |
BR112022011271A2 (pt) | 2019-12-10 | 2022-09-06 | Novozymes As | Célula hospedeira recombinante, composição, métodos para produzir um derivado de uma célula e um produto de fermentação, e, uso de uma célula hospedeira recombinante |
WO2021126966A1 (fr) | 2019-12-16 | 2021-06-24 | Novozymes A/S | Procédés de production de produits de fermentation |
US20240228996A1 (en) | 2020-02-10 | 2024-07-11 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
EP4156944A1 (fr) | 2020-05-29 | 2023-04-05 | Novozymes A/S | Pâtes enrichies en protéines de légume sec et/ou de légumineuses et produits cuits comprenant une lipase |
AU2021370998A1 (en) | 2020-11-02 | 2023-05-25 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2023225459A2 (fr) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions et procédés de prévention, de traitement, de suppression et/ou d'élimination d'infestations et d'infections phytopathogènes |
CN117795089A (zh) | 2021-06-07 | 2024-03-29 | 诺维信公司 | 用于改善的乙醇发酵的工程化微生物 |
MX2024003691A (es) | 2021-09-27 | 2024-06-26 | Int N&H Denmark Aps | Composiciones de aditivos de piensos y métodos para usar los mismos. |
WO2023225510A1 (fr) | 2022-05-17 | 2023-11-23 | Dupont Nutrition Biosciences Aps | Additif alimentaire comprenant des combinaisons d'enzymes |
WO2024137246A1 (fr) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Polypeptides de la famille 1 d'estérase de glucide (ce1) présentant une activité d'estérase d'acide férulique et/ou d'estérase d'acétyl xylane et polynucléotides codant pour ceux-ci |
WO2024137248A1 (fr) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions contenant des arabinofuranosidases et une xylanase, et leur utilisation pour augmenter la solubilisation de fibres hémicellulosiques |
WO2024137252A1 (fr) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Procédé de réduction de la viscosité du sirop à la fin d'un processus de production d'un produit de fermentation |
WO2024137704A2 (fr) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Procédés de production de produits de fermentation faisant appel à des enzymes de dégradation de fibres avec levure modifiée |
WO2024137250A1 (fr) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Polypeptides de la famille 3 de gludice estérase (ce3) présentant une activité acétyl xylane estérase et polynucléotides codant pour ceux-ci |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IL61982A (en) * | 1980-02-15 | 1984-01-31 | Cpc International Inc | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes |
FI64813C (fi) * | 1980-12-31 | 1984-01-10 | Ilkka Antero Palva | Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer |
AU1545683A (en) * | 1982-06-10 | 1983-12-15 | Unisearch Limited | Engineered e. coli to produce glucoamylase |
-
1984
- 1984-01-19 NO NO840200A patent/NO840200L/no unknown
- 1984-01-25 FI FI840295A patent/FI840295A/fi not_active Application Discontinuation
- 1984-01-26 DK DK36384A patent/DK36384A/da not_active Application Discontinuation
- 1984-01-26 AU AU24993/84A patent/AU567031B2/en not_active Ceased
- 1984-01-26 EP EP84100834A patent/EP0126206B1/fr not_active Expired - Lifetime
- 1984-01-26 EP EP87116406A patent/EP0292609B1/fr not_active Expired - Lifetime
- 1984-01-26 DE DE8787116406T patent/DE3485723D1/de not_active Expired - Fee Related
- 1984-01-26 DE DE8484100834T patent/DE3484521D1/de not_active Expired - Fee Related
- 1984-01-26 WO PCT/US1984/000122 patent/WO1984002921A2/fr unknown
- 1984-01-26 DE DE198484100834T patent/DE126206T1/de active Pending
- 1984-01-29 IL IL70811A patent/IL70811A/xx unknown
- 1984-01-30 IE IE840214A patent/IE840214L/xx unknown
- 1984-01-31 NZ NZ207000A patent/NZ207000A/en unknown
Also Published As
Publication number | Publication date |
---|---|
AU2499384A (en) | 1984-08-15 |
EP0126206A2 (fr) | 1984-11-28 |
FI840295A0 (fi) | 1984-01-25 |
DK36384D0 (da) | 1984-01-26 |
EP0292609A1 (fr) | 1988-11-30 |
DE126206T1 (de) | 1985-05-23 |
IL70811A0 (en) | 1984-04-30 |
EP0126206A3 (en) | 1985-03-06 |
IL70811A (en) | 1991-08-16 |
WO1984002921A2 (fr) | 1984-08-02 |
IE840214L (en) | 1984-07-28 |
DE3485723D1 (de) | 1992-06-17 |
EP0292609B1 (fr) | 1992-05-13 |
FI840295A (fi) | 1984-07-29 |
WO1984002921A3 (fr) | 1984-09-27 |
DK36384A (da) | 1984-07-29 |
NZ207000A (en) | 1988-01-08 |
AU567031B2 (en) | 1987-11-05 |
DE3484521D1 (de) | 1991-06-06 |
EP0126206B1 (fr) | 1991-05-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
NO840200L (no) | Glukoamylase cdna. | |
US4794175A (en) | Glucoamylase CDNA | |
JP4562786B2 (ja) | サッカロミセスセレビシアエによるジスルフィド結合をもつ組換えタンパク質の産生を増加させる方法 | |
JP2868179B2 (ja) | 糸状菌類のプロモーターを用いるタンパク質の製造方法 | |
Dohmen et al. | Cloning of the Schwanniomyces occidentalis glucoamylase gene (GAM1) and its expression in Saccharomyces cerevisiae | |
EP0768383B1 (fr) | Utilisation du promoteur de la glucoamylase d'Aspergillus | |
Cole et al. | Stable expression of Aspercillus awamori glucoamylase in distiller's yeast | |
JPH10501414A (ja) | areA遺伝子が修飾された真菌及びアスペルギルス オリザエからのareA遺伝子 | |
CN104232611B (zh) | 一种重组布氏白僵菌蛋白酶k及其工业化生产与纯化方法 | |
CA2198828C (fr) | Methode pour accroitre la thermostabilite des enzymes cellulasiques par elimination des domaines de fixation de la cellulose | |
JP2002515252A (ja) | 糸状菌変異体細胞においてポリペプチドを生産するための方法 | |
US5834191A (en) | Production of heterologous peptides | |
JPH08266290A (ja) | フサリウムオキシスポラムからのペニシリンvアミドヒドロラーゼ遺伝子 | |
EP0722501B1 (fr) | Pullulanase clonee | |
US5045463A (en) | DNA expression vector and use thereof | |
DK176017B1 (da) | Promotorer fra filamentöse fungi samt anvendelse deraf | |
JPH09505989A (ja) | 菌類からのα‐1,4‐グルカンリアーゼ、その精製、遺伝子クローニングおよび微生物での発現 | |
US5541084A (en) | Hybrid yeast promoter comprising an ENO2 UAS and TATA region and an additional UAS located between said ENO2 UAS and TATA region | |
NZ219435A (en) | Production of glucoamylase by recombinant techniques | |
DK175554B1 (da) | Fremgangsmåde til fremstilling af et polypeptid i en filamentös fungusværtscelle | |
IE64478B1 (en) | Process and signal sequence for the extracellular production of proteinaceous material | |
JPH08256770A (ja) | ピキア属酵母由来のプロテアーゼ |