NO313758B1 - Fremgangsmåte for fremstilling av difteritoksin eller CRM- protein - Google Patents
Fremgangsmåte for fremstilling av difteritoksin eller CRM- protein Download PDFInfo
- Publication number
- NO313758B1 NO313758B1 NO19940774A NO940774A NO313758B1 NO 313758 B1 NO313758 B1 NO 313758B1 NO 19940774 A NO19940774 A NO 19940774A NO 940774 A NO940774 A NO 940774A NO 313758 B1 NO313758 B1 NO 313758B1
- Authority
- NO
- Norway
- Prior art keywords
- plasmid
- crmi97
- diphtheria toxin
- protein
- gene
- Prior art date
Links
- 108010053187 Diphtheria Toxin Proteins 0.000 title claims abstract description 50
- 102000016607 Diphtheria Toxin Human genes 0.000 title claims abstract description 41
- 101150044687 crm gene Proteins 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000008569 process Effects 0.000 title claims description 4
- 238000002360 preparation method Methods 0.000 title description 3
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 69
- 239000013612 plasmid Substances 0.000 claims abstract description 54
- 244000005700 microbiome Species 0.000 claims abstract description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 38
- 238000004519 manufacturing process Methods 0.000 claims description 19
- 241000186216 Corynebacterium Species 0.000 claims description 11
- 230000010076 replication Effects 0.000 claims description 10
- 239000003550 marker Substances 0.000 claims description 8
- 238000004520 electroporation Methods 0.000 claims description 6
- 101100298079 African swine fever virus (strain Badajoz 1971 Vero-adapted) pNG2 gene Proteins 0.000 claims description 4
- 230000009466 transformation Effects 0.000 claims description 4
- 108010034055 CRM45 fragment of diphtheria toxin Proteins 0.000 claims description 3
- 206010013023 diphtheria Diseases 0.000 claims description 3
- 239000003053 toxin Substances 0.000 claims description 2
- 231100000765 toxin Toxicity 0.000 claims description 2
- 238000011426 transformation method Methods 0.000 claims 1
- 241000186227 Corynebacterium diphtheriae Species 0.000 abstract description 29
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 14
- 229910052742 iron Inorganic materials 0.000 abstract description 7
- 230000001131 transforming effect Effects 0.000 abstract description 2
- 108010071134 CRM197 (non-toxic variant of diphtheria toxin) Proteins 0.000 abstract 3
- 239000013604 expression vector Substances 0.000 abstract 1
- 230000014616 translation Effects 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 32
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- 102000036639 antigens Human genes 0.000 description 17
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 15
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- 229940047650 haemophilus influenzae Drugs 0.000 description 2
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- 229940031000 streptococcus pneumoniae Drugs 0.000 description 2
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- ITFICYZHWXDVMU-IPTZIORSSA-N (2S)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-4-amino-2-[[(2S,3S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-6-amino-2-[[(2S,3S)-2-[[(2S)-2-[[(2R)-2-[[(2S)-1-[(2S)-2-[[(2S,3R)-2-[[(2S,3S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-amino-4-carboxybutanoyl]amino]acetyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxybutanoyl]amino]-3-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]-4-methylpentanoyl]pyrrolidine-2-carbonyl]amino]-3-sulfanylpropanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-3-methylpentanoyl]amino]hexanoyl]amino]-5-oxopentanoyl]amino]-3-phenylpropanoyl]amino]-3-methylpentanoyl]amino]-4-oxobutanoyl]amino]-4-methylsulfanylbutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-oxopentanoyl]amino]pentanedioic acid Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC2=CNC3=CC=CC=C32)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CS)NC(=O)[C@@H]4CCCN4C(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(=O)O)N ITFICYZHWXDVMU-IPTZIORSSA-N 0.000 description 1
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 1
- 108010068327 4-hydroxyphenylpyruvate dioxygenase Proteins 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000223836 Babesia Species 0.000 description 1
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- 241000588832 Bordetella pertussis Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 201000006082 Chickenpox Diseases 0.000 description 1
- 241000193449 Clostridium tetani Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 108010060123 Conjugate Vaccines Proteins 0.000 description 1
- 241000711573 Coronaviridae Species 0.000 description 1
- 241000595586 Coryne Species 0.000 description 1
- 241001337994 Cryptococcus <scale insect> Species 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 241000223924 Eimeria Species 0.000 description 1
- 241000991587 Enterovirus C Species 0.000 description 1
- 108091006027 G proteins Proteins 0.000 description 1
- 102000030782 GTP binding Human genes 0.000 description 1
- 108091000058 GTP-Binding Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
- 241000228402 Histoplasma Species 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- 241000222722 Leishmania <genus> Species 0.000 description 1
- 101710085938 Matrix protein Proteins 0.000 description 1
- 241000712079 Measles morbillivirus Species 0.000 description 1
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- 101710127721 Membrane protein Proteins 0.000 description 1
- 208000002430 Multiple chemical sensitivity Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 101710116435 Outer membrane protein Proteins 0.000 description 1
- 241001631646 Papillomaviridae Species 0.000 description 1
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 1
- 241000224016 Plasmodium Species 0.000 description 1
- 241000233870 Pneumocystis Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
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- 108020005091 Replication Origin Proteins 0.000 description 1
- 241000710799 Rubella virus Species 0.000 description 1
- 241000242678 Schistosoma Species 0.000 description 1
- 241000700584 Simplexvirus Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 102400000368 Surface protein Human genes 0.000 description 1
- 208000000389 T-cell leukemia Diseases 0.000 description 1
- 208000028530 T-cell lymphoblastic leukemia/lymphoma Diseases 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 241000223996 Toxoplasma Species 0.000 description 1
- 241000223104 Trypanosoma Species 0.000 description 1
- 206010046980 Varicella Diseases 0.000 description 1
- 241000607626 Vibrio cholerae Species 0.000 description 1
- 241000710772 Yellow fever virus Species 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
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- 150000001413 amino acids Chemical group 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
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- 239000004220 glutamic acid Substances 0.000 description 1
- 229940045808 haemophilus influenzae type b Drugs 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
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- 231100000283 hepatitis Toxicity 0.000 description 1
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- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
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- 229920001184 polypeptide Polymers 0.000 description 1
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- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
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- 238000006467 substitution reaction Methods 0.000 description 1
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- 231100000563 toxic property Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
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- 229940118696 vibrio cholerae Drugs 0.000 description 1
- 229940051021 yellow-fever virus Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2728393A | 1993-03-05 | 1993-03-05 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO940774D0 NO940774D0 (no) | 1994-03-04 |
| NO940774L NO940774L (no) | 1994-09-06 |
| NO313758B1 true NO313758B1 (no) | 2002-11-25 |
Family
ID=21836767
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO19940774A NO313758B1 (no) | 1993-03-05 | 1994-03-04 | Fremgangsmåte for fremstilling av difteritoksin eller CRM- protein |
Country Status (21)
| Country | Link |
|---|---|
| US (1) | US5614382A (fr) |
| EP (1) | EP0616034B1 (fr) |
| JP (1) | JP4144813B2 (fr) |
| KR (1) | KR100316004B1 (fr) |
| CN (1) | CN1100757A (fr) |
| AT (1) | ATE280235T1 (fr) |
| AU (1) | AU686126B2 (fr) |
| BR (1) | BR1100634A (fr) |
| CA (1) | CA2116914C (fr) |
| CZ (1) | CZ39394A3 (fr) |
| DE (1) | DE69434079T2 (fr) |
| DK (1) | DK0616034T3 (fr) |
| ES (1) | ES2231770T3 (fr) |
| FI (1) | FI112090B (fr) |
| HU (1) | HUT71320A (fr) |
| IL (1) | IL108822A (fr) |
| NO (1) | NO313758B1 (fr) |
| NZ (1) | NZ260027A (fr) |
| PT (1) | PT616034E (fr) |
| SK (1) | SK24094A3 (fr) |
| ZA (1) | ZA941548B (fr) |
Families Citing this family (123)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996032137A2 (fr) * | 1995-04-14 | 1996-10-17 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Techniques de declenchement d'une tolerance immunitaire a l'aide d'immunotoxine |
| US7288254B2 (en) | 1995-10-30 | 2007-10-30 | The United States Of America As Represented By The Secretary, Department Of Health And Human Services, Nih | Use of immunotoxins to induce immune tolerance to pancreatic islet transplantation |
| US7696338B2 (en) | 1995-10-30 | 2010-04-13 | The United States Of America As Represented By The Department Of Health And Human Services | Immunotoxin fusion proteins and means for expression thereof |
| US7517527B2 (en) | 1995-10-30 | 2009-04-14 | The United States Of America As Represented By The Department Of Health And Human Services | Immunotoxin with in vivo T cell suppressant activity and methods of use |
| US7125553B1 (en) | 1996-04-15 | 2006-10-24 | The United States of America as represented by the Department of Health and Human Services c/o Centers for Disease Control and Prevention | Methods of inducing immune tolerance using immunotoxins |
| AU736501B2 (en) * | 1997-03-05 | 2001-07-26 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Novel vectors and expression methods for producing mutant proteins |
| EP1015496B1 (fr) | 1997-03-05 | 2009-06-17 | THE UNITED STATES GOVERNMENT as represented by THE DEPARTMENT OF HEALTH AND HUMAN SERVICES | Immunotoxines divalentes contre les cellules t et leur utilisation |
| US6676943B1 (en) | 1997-04-24 | 2004-01-13 | Regents Of The University Of Minnesota | Human complement C3-degrading protein from Streptococcus pneumoniae |
| CN1086945C (zh) * | 1997-06-03 | 2002-07-03 | 胡章英 | 白喉口服液 |
| GB9923060D0 (en) * | 1999-09-29 | 1999-12-01 | Chiron Spa | Vaccine |
| MX339524B (es) | 2001-10-11 | 2016-05-30 | Wyeth Corp | Composiciones inmunogenicas novedosas para la prevencion y tratamiento de enfermedad meningococica. |
| GB0211118D0 (en) * | 2002-05-15 | 2002-06-26 | Polonelli Luciano | Vaccines |
| US20040096461A1 (en) * | 2002-07-30 | 2004-05-20 | Baxter Healthcare Corporation | Chimeric multivalent polysaccharide conjugate vaccines |
| US7785608B2 (en) * | 2002-08-30 | 2010-08-31 | Wyeth Holdings Corporation | Immunogenic compositions for the prevention and treatment of meningococcal disease |
| US7301554B2 (en) * | 2002-09-20 | 2007-11-27 | Ricoh Company, Ltd. | Light scanning device, scanning line adjusting method, scanning line adjusting control method, image forming apparatus, and image forming method |
| US8227403B2 (en) | 2003-12-17 | 2012-07-24 | Wyeth Llc | A-β immunogenic peptide carrier conjugates and methods of producing same |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CH660375A5 (it) * | 1983-02-08 | 1987-04-15 | Sclavo Spa | Procedimento per la produzione di proteine correlate alla tossina difterica. |
| FR2549855B1 (fr) * | 1983-07-29 | 1987-03-27 | Grp Genie Genetique | Sequence nucleotidique codant pour le peptide signal de la toxine diphterique, vecteur contenant cette sequence nucleotidique, leur application a la transformation de micro-organismes et compositions peptidiques obtenues |
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1994
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| ATE280235T1 (de) | 2004-11-15 |
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| BR1100634A (pt) | 1999-12-07 |
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| EP0616034A3 (en) | 1996-10-16 |
| HU9400657D0 (en) | 1994-05-30 |
| CA2116914A1 (fr) | 1994-09-06 |
| FI941050A0 (fi) | 1994-03-04 |
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