IT1398927B1 - Espressione batterica di un gene artificiale per la produzione di crm197 e derivati. - Google Patents

Espressione batterica di un gene artificiale per la produzione di crm197 e derivati.

Info

Publication number
IT1398927B1
IT1398927B1 ITFI2009A000137A ITFI20090137A IT1398927B1 IT 1398927 B1 IT1398927 B1 IT 1398927B1 IT FI2009A000137 A ITFI2009A000137 A IT FI2009A000137A IT FI20090137 A ITFI20090137 A IT FI20090137A IT 1398927 B1 IT1398927 B1 IT 1398927B1
Authority
IT
Italy
Prior art keywords
crm197
derivatives
production
bacterial expression
artificial gene
Prior art date
Application number
ITFI2009A000137A
Other languages
English (en)
Inventor
Piero Baglioni
Alejandro Hochkoeppler
Alessandra Stefan
Original Assignee
Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ITFI2009A000137A priority Critical patent/IT1398927B1/it
Application filed by Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi filed Critical Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi
Priority to CN2010800282296A priority patent/CN102459317A/zh
Priority to JP2012516956A priority patent/JP2012531198A/ja
Priority to PCT/IB2010/052910 priority patent/WO2010150230A1/en
Priority to NZ597774A priority patent/NZ597774A/xx
Priority to AU2010264044A priority patent/AU2010264044A1/en
Priority to US13/379,724 priority patent/US20120128727A1/en
Priority to MX2011013476A priority patent/MX2011013476A/es
Priority to EP10742260A priority patent/EP2445930A1/en
Priority to KR1020127001814A priority patent/KR20120095837A/ko
Priority to CA2766349A priority patent/CA2766349A1/en
Publication of ITFI20090137A1 publication Critical patent/ITFI20090137A1/it
Application granted granted Critical
Publication of IT1398927B1 publication Critical patent/IT1398927B1/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
ITFI2009A000137A 2009-06-25 2009-06-25 Espressione batterica di un gene artificiale per la produzione di crm197 e derivati. IT1398927B1 (it)

Priority Applications (11)

Application Number Priority Date Filing Date Title
ITFI2009A000137A IT1398927B1 (it) 2009-06-25 2009-06-25 Espressione batterica di un gene artificiale per la produzione di crm197 e derivati.
JP2012516956A JP2012531198A (ja) 2009-06-25 2010-06-25 Ｃｒｍ１９７及びその誘導体の産生のための人工遺伝子の細菌発現
PCT/IB2010/052910 WO2010150230A1 (en) 2009-06-25 2010-06-25 Bacterial expression of an artificial gene for the production of crm197 and its derivatives
NZ597774A NZ597774A (en) 2009-06-25 2010-06-25 Bacterial expression of an artificial gene for the production of cross reactive material 197 and its derivatives
CN2010800282296A CN102459317A (zh) 2009-06-25 2010-06-25 用于crm197及其衍生物的生产的人工基因的细菌表达
AU2010264044A AU2010264044A1 (en) 2009-06-25 2010-06-25 Bacterial expression of an artificial gene for the production of CRM197 and its derivatives
US13/379,724 US20120128727A1 (en) 2009-06-25 2010-06-25 Bacterial expression of an artificial gene for the production of crm197 and its derivatives
MX2011013476A MX2011013476A (es) 2009-06-25 2010-06-25 Expresion bacteriana de un gen artificial para la produccion de material 197 de reaccion cruzada (crm197) y sus derivados.
EP10742260A EP2445930A1 (en) 2009-06-25 2010-06-25 Bacterial expression of an artificial gene for the production of crm197 and its derivatives
KR1020127001814A KR20120095837A (ko) 2009-06-25 2010-06-25 Crm197 및 그 유도체의 제조를 위한 합성 유전자의 박테리아성 발현
CA2766349A CA2766349A1 (en) 2009-06-25 2010-06-25 Bacterial expression of an artificial gene for the production of crm197 and its derivatives

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITFI2009A000137A IT1398927B1 (it) 2009-06-25 2009-06-25 Espressione batterica di un gene artificiale per la produzione di crm197 e derivati.

Publications (2)

Publication Number Publication Date
ITFI20090137A1 ITFI20090137A1 (it) 2010-12-26
IT1398927B1 true IT1398927B1 (it) 2013-03-28

Family

ID=41404254

Family Applications (1)

Application Number Title Priority Date Filing Date
ITFI2009A000137A IT1398927B1 (it) 2009-06-25 2009-06-25 Espressione batterica di un gene artificiale per la produzione di crm197 e derivati.

Country Status (11)

Country Link
US (1) US20120128727A1 (it)
EP (1) EP2445930A1 (it)
JP (1) JP2012531198A (it)
KR (1) KR20120095837A (it)
CN (1) CN102459317A (it)
AU (1) AU2010264044A1 (it)
CA (1) CA2766349A1 (it)
IT (1) IT1398927B1 (it)
MX (1) MX2011013476A (it)
NZ (1) NZ597774A (it)
WO (1) WO2010150230A1 (it)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10287330B2 (en) 2012-12-27 2019-05-14 Glaxosmithkline Biologicals S.A. Methods and compositions relating to CRM197
US10093704B2 (en) 2014-01-31 2018-10-09 Fina Biosolutions, Llc Expression and purification of CRM197 and related proteins
US11060123B2 (en) 2014-01-31 2021-07-13 Fina Biosolutions, Llc Production of soluble recombinant protein without n-terminal methionine
US10479820B2 (en) 2014-03-03 2019-11-19 Scarab Genomics, Llc Enhanced production of recombinant CRM197 in E. coli
WO2015165840A1 (en) 2014-04-28 2015-11-05 Novartis Ag Antibiotic-free method for selection of transformed bacteria
EP3221339A1 (en) * 2014-11-20 2017-09-27 Biological E Limited Codon optimized polynucleotide for high level expression of crm197
CN106520788B (zh) * 2016-12-23 2021-05-28 无锡佰翱得生物科学有限公司 一种重组表达pcv2的方法
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
KR101908590B1 (ko) * 2017-02-01 2018-10-16 (주)포바이오코리아 Crm197의 용해성 단백질 발현 및 정제 방법
CN111201321A (zh) 2017-09-29 2020-05-26 陶氏环球技术有限责任公司 基因修饰的异丙基苹果酸异构酶酶复合物及用其制备伸长的2-酮酸和c5-c10化合物的方法
KR102078714B1 (ko) * 2018-02-02 2020-02-19 (주)포바이오코리아 봉입체로 발현된 불용성 crm197 단백질로부터 활성 crm197 효율적인 회수 및 정제 방법
KR102059619B1 (ko) 2018-04-17 2019-12-26 강원대학교산학협력단 글루코스-자일로스 혼합당의 동시발효가 가능한 돌연변이 균주
CN109486800B (zh) * 2018-11-21 2019-12-13 珠海冀百康生物科技有限公司 一种新型赖氨酰肽链内切酶及其制备方法
CN111378047B (zh) * 2018-12-28 2022-12-16 复旦大学 一种提高蛋白表达的融合标签蛋白及其应用
BE1029145B1 (fr) * 2021-02-26 2022-09-27 Curavac Europe Methode de production d'une forme periplasmique de la proteine crm197
GB202108650D0 (en) * 2021-06-17 2021-08-04 Inst De Medicina Molecular Joaeo Lobo Antunes Production of cross-reactive material 197 fusion proteins

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH660375A5 (it) 1983-02-08 1987-04-15 Sclavo Spa Procedimento per la produzione di proteine correlate alla tossina difterica.
NZ253065A (en) 1992-05-23 1996-10-28 Smithkline Beecham Biolog Combination vaccines comprising hepatitis b surface antigens and other antigens wherein aluminium phosphate adjuvant is used to adsorb the hepatitis antigen
DE69434079T2 (de) 1993-03-05 2005-02-24 Wyeth Holdings Corp. Plasmid zur Herstellung von CRM-Protein und Diphtherie-Toxin
SI1082965T1 (sl) 1995-06-23 2009-08-31 Glaxosmithkline Biolog Sa Sestavek cepiva, ki vsebuje antigen konjugata polisaharida, adsorbiranega na aluminijevem fosfatu
GB9904582D0 (en) 1999-02-26 1999-04-21 Nycomed Imaging As Process
US6821755B2 (en) * 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
KR100401423B1 (ko) 2001-01-10 2003-10-17 주식회사 엘지생명과학 혼합 백신의 제조 방법
FR2827606A1 (fr) * 2001-07-20 2003-01-24 Pf Medicament Nouveaux derives d'anatoxines diphteriques et leur utilisation comme porteur
JP4203742B2 (ja) * 2002-10-15 2009-01-07 財団法人阪大微生物病研究会 制癌剤
GB0505996D0 (en) 2005-03-23 2005-04-27 Glaxosmithkline Biolog Sa Fermentation process
US20060270600A1 (en) * 2005-05-26 2006-11-30 Eisuke Mekada Anti-cancer agents
CN100999548B (zh) * 2006-01-10 2010-09-08 海南天源康泽医药科技有限公司 白喉毒素突变体crm197及其制备方法
CA2649038A1 (en) * 2006-05-30 2007-12-13 Dow Global Technolgies Inc. Codon optimization method
CN100513568C (zh) * 2006-06-01 2009-07-15 刘建宁 编码重组人尿钠素的基因及利用该基因生产重组人尿钠素的方法
CN100532548C (zh) * 2007-02-14 2009-08-26 马润林 一种提高人乳头瘤病毒l1蛋白原核表达产率的方法

Also Published As

Publication number Publication date
NZ597774A (en) 2013-02-22
ITFI20090137A1 (it) 2010-12-26
WO2010150230A1 (en) 2010-12-29
CA2766349A1 (en) 2010-12-29
US20120128727A1 (en) 2012-05-24
AU2010264044A1 (en) 2012-02-16
CN102459317A (zh) 2012-05-16
MX2011013476A (es) 2012-04-11
JP2012531198A (ja) 2012-12-10
KR20120095837A (ko) 2012-08-29
WO2010150230A8 (en) 2011-03-03
EP2445930A1 (en) 2012-05-02

Similar Documents

Publication Publication Date Title
IT1398927B1 (it) Espressione batterica di un gene artificiale per la produzione di crm197 e derivati.
SMT201600198B (it) Derivati di 1,2,4-triazina-4-ammina
IT1393929B1 (it) Procedimento per la produzione di bio-olio da biomassa
BRPI0906611A2 (pt) Produtos implantáveis compreendendo nanopartículas.
IT1400823B1 (it) Carcassa di turbina.
BR112012000421A2 (pt) composições e métodos para intensificar a produção de um produto biológico.
IT1391099B1 (it) Procedimento per la produzione di zuccheri da biomassa
SMT201400114B (it) Derivati di 2-arilimidazolo come inibitori dell'enzima pde10a
IT1393061B1 (it) Procedimento integrato per la produzione di bio-olio da microrganismi
BRPI0925037A2 (pt) Composição contendo piceatanol e método de produzir a composição contendo piceatanol.
BRPI1016117A8 (pt) derivados de isoxazol-isoxazol e isoxazol-isotiazol.
IT1392910B1 (it) Procedimento per la produzione di lipidi da biomassa
SMT201400080B (it) Derivati di pirazinoossazepina
IT1391230B1 (it) Metodo per la produzione di una struttura antiproiettile e struttura antiproiettile
IT1403244B1 (it) Procedimento per la produzione di lipidi da biomassa.
IT1394398B1 (it) Procedimento per la produzione di zuccheri da biomassa
BRPI1016020A2 (pt) linha cirúrgica que compreende células e método de produção da linha.
ITTO20110857A1 (it) Procedimento per la produzione di cicloesanonossina.
ITRM20090620A1 (it) Protesi vascolare perfezionata e relativo metodo di realizzazione.
BRPI0903701A2 (pt) implante
FR2946246B1 (fr) Fauteuil roulant.
IT1399809B1 (it) Palloncino per cateteri e relativo procedimento di produzione
FR2949966B1 (fr) Implant intraoculaire.
EE201100056A (et) Pumpamisssteem ja meetod
IT1394624B1 (it) Protesi per anuloplastica mitralica.