MX2011013476A - Expresion bacteriana de un gen artificial para la produccion de material 197 de reaccion cruzada (crm197) y sus derivados. - Google Patents

Expresion bacteriana de un gen artificial para la produccion de material 197 de reaccion cruzada (crm197) y sus derivados.

Info

Publication number
MX2011013476A
MX2011013476A MX2011013476A MX2011013476A MX2011013476A MX 2011013476 A MX2011013476 A MX 2011013476A MX 2011013476 A MX2011013476 A MX 2011013476A MX 2011013476 A MX2011013476 A MX 2011013476A MX 2011013476 A MX2011013476 A MX 2011013476A
Authority
MX
Mexico
Prior art keywords
crm197
production
derivatives
bacterial expression
artificial gene
Prior art date
Application number
MX2011013476A
Other languages
English (en)
Inventor
Piero Baglioni
Alejandro Hochkoeppler
Alessandra Stefan
Original Assignee
Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi filed Critical Consorzio Interuniversitario Per Lo Sviluppo Dei Sistemi A Grande Interfase Csgi
Publication of MX2011013476A publication Critical patent/MX2011013476A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Vascular Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Medicinal Preparation (AREA)

Abstract

La presente invención se refiere a secuencias de polinucleótido que comprenden la SEQ ID N° 1 que codifica para CRM197 y se optimiza para su expresión en E. coli. La invención se refiere en consecuencia a un método para la producción de CRM197 en E. coli mediante una proteína de fusión CRM197-marca.
MX2011013476A 2009-06-25 2010-06-25 Expresion bacteriana de un gen artificial para la produccion de material 197 de reaccion cruzada (crm197) y sus derivados. MX2011013476A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITFI2009A000137A IT1398927B1 (it) 2009-06-25 2009-06-25 Espressione batterica di un gene artificiale per la produzione di crm197 e derivati.
PCT/IB2010/052910 WO2010150230A1 (en) 2009-06-25 2010-06-25 Bacterial expression of an artificial gene for the production of crm197 and its derivatives

Publications (1)

Publication Number Publication Date
MX2011013476A true MX2011013476A (es) 2012-04-11

Family

ID=41404254

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2011013476A MX2011013476A (es) 2009-06-25 2010-06-25 Expresion bacteriana de un gen artificial para la produccion de material 197 de reaccion cruzada (crm197) y sus derivados.

Country Status (11)

Country Link
US (1) US20120128727A1 (es)
EP (1) EP2445930A1 (es)
JP (1) JP2012531198A (es)
KR (1) KR20120095837A (es)
CN (1) CN102459317A (es)
AU (1) AU2010264044A1 (es)
CA (1) CA2766349A1 (es)
IT (1) IT1398927B1 (es)
MX (1) MX2011013476A (es)
NZ (1) NZ597774A (es)
WO (1) WO2010150230A1 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10287330B2 (en) 2012-12-27 2019-05-14 Glaxosmithkline Biologicals S.A. Methods and compositions relating to CRM197
US11060123B2 (en) 2014-01-31 2021-07-13 Fina Biosolutions, Llc Production of soluble recombinant protein without n-terminal methionine
WO2015117093A1 (en) * 2014-01-31 2015-08-06 Fina Biosolutions, Llc Expression and purification of crm197 and related proteins
JP6473460B2 (ja) * 2014-03-03 2019-02-20 スカラブ ゲノミクス， エルエルシー 大腸菌における組換えｃｒｍ１９７の産生強化
WO2015165840A1 (en) 2014-04-28 2015-11-05 Novartis Ag Antibiotic-free method for selection of transformed bacteria
CN107109416A (zh) * 2014-11-20 2017-08-29 生物E有限公司 用于crm197的高水平表达的密码子优化多核苷酸
CN106520788B (zh) * 2016-12-23 2021-05-28 无锡佰翱得生物科学有限公司 一种重组表达pcv2的方法
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
KR101908590B1 (ko) 2017-02-01 2018-10-16 (주)포바이오코리아 Crm197의 용해성 단백질 발현 및 정제 방법
CA3076748A1 (en) 2017-09-29 2019-04-04 Dow Global Technologies Llc Genetically modified isopropylmalate isomerase enzyme complexes and processes to prepare elongated 2-ketoacids and c5-c10 compounds therewith
KR102078714B1 (ko) * 2018-02-02 2020-02-19 (주)포바이오코리아 봉입체로 발현된 불용성 crm197 단백질로부터 활성 crm197 효율적인 회수 및 정제 방법
KR102059619B1 (ko) 2018-04-17 2019-12-26 강원대학교산학협력단 글루코스-자일로스 혼합당의 동시발효가 가능한 돌연변이 균주
CN109486800B (zh) * 2018-11-21 2019-12-13 珠海冀百康生物科技有限公司 一种新型赖氨酰肽链内切酶及其制备方法
CN111378047B (zh) * 2018-12-28 2022-12-16 复旦大学 一种提高蛋白表达的融合标签蛋白及其应用
BE1029145B1 (fr) * 2021-02-26 2022-09-27 Curavac Europe Methode de production d'une forme periplasmique de la proteine crm197
GB202108650D0 (en) * 2021-06-17 2021-08-04 Inst De Medicina Molecular Joaeo Lobo Antunes Production of cross-reactive material 197 fusion proteins

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH660375A5 (it) 1983-02-08 1987-04-15 Sclavo Spa Procedimento per la produzione di proteine correlate alla tossina difterica.
CZ283910B6 (cs) 1992-05-23 1998-07-15 Smithkline Beecham Biologicals (S.A.) Kombinovaný očkovací prostředek, způsob jeho výroby a použití fosforečnanu hlinitého jako pomocného prostředku
DE69434079T2 (de) 1993-03-05 2005-02-24 Wyeth Holdings Corp. Plasmid zur Herstellung von CRM-Protein und Diphtherie-Toxin
EP0833662B2 (en) 1995-06-23 2011-01-26 SmithKline Beecham Biologicals S.A. A vaccine composition comprsing a Haemophilus influenzae B polysaccharide conjugate antigen adsorbed onto aluminium phosphate
GB9904582D0 (en) 1999-02-26 1999-04-21 Nycomed Imaging As Process
US6821755B2 (en) * 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
KR100401423B1 (ko) 2001-01-10 2003-10-17 주식회사 엘지생명과학 혼합 백신의 제조 방법
FR2827606A1 (fr) * 2001-07-20 2003-01-24 Pf Medicament Nouveaux derives d'anatoxines diphteriques et leur utilisation comme porteur
JP4203742B2 (ja) * 2002-10-15 2009-01-07 財団法人阪大微生物病研究会 制癌剤
GB0505996D0 (en) 2005-03-23 2005-04-27 Glaxosmithkline Biolog Sa Fermentation process
US20060270600A1 (en) * 2005-05-26 2006-11-30 Eisuke Mekada Anti-cancer agents
CN100999548B (zh) * 2006-01-10 2010-09-08 海南天源康泽医药科技有限公司 白喉毒素突变体crm197及其制备方法
KR20090018799A (ko) * 2006-05-30 2009-02-23 다우 글로벌 테크놀로지스 인크. 코돈 최적화 방법
CN100513568C (zh) * 2006-06-01 2009-07-15 刘建宁 编码重组人尿钠素的基因及利用该基因生产重组人尿钠素的方法
CN100532548C (zh) * 2007-02-14 2009-08-26 马润林 一种提高人乳头瘤病毒l1蛋白原核表达产率的方法

Also Published As

Publication number Publication date
IT1398927B1 (it) 2013-03-28
KR20120095837A (ko) 2012-08-29
EP2445930A1 (en) 2012-05-02
AU2010264044A1 (en) 2012-02-16
US20120128727A1 (en) 2012-05-24
ITFI20090137A1 (it) 2010-12-26
WO2010150230A8 (en) 2011-03-03
CA2766349A1 (en) 2010-12-29
CN102459317A (zh) 2012-05-16
NZ597774A (en) 2013-02-22
JP2012531198A (ja) 2012-12-10
WO2010150230A1 (en) 2010-12-29

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