MX2011005074A - Una fitasa escherichia coli no-k12 termotolerante y su produccion. - Google Patents

Una fitasa escherichia coli no-k12 termotolerante y su produccion.

Info

Publication number
MX2011005074A
MX2011005074A MX2011005074A MX2011005074A MX2011005074A MX 2011005074 A MX2011005074 A MX 2011005074A MX 2011005074 A MX2011005074 A MX 2011005074A MX 2011005074 A MX2011005074 A MX 2011005074A MX 2011005074 A MX2011005074 A MX 2011005074A
Authority
MX
Mexico
Prior art keywords
thermotolerant
escherichia coli
production
phytase gene
coli phytase
Prior art date
Application number
MX2011005074A
Other languages
English (en)
Other versions
MX307031B (es
Inventor
Xiuyun Ye
Renkaun Li
Weigang Jin
Caifang Chen
Original Assignee
Fujian Fuda Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujian Fuda Biotech Co Ltd filed Critical Fujian Fuda Biotech Co Ltd
Publication of MX2011005074A publication Critical patent/MX2011005074A/es
Publication of MX307031B publication Critical patent/MX307031B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Esta invención se relaciona con la clonación y secuenciación del gene de fitasa termotolerante de la cepa de Escherichia coli Non-K12, ATCC 9637, expresión del gene de fitasa en el sistema de expresión de la Escherichia coli, uso del codón optimizado y expresión en Pichia Pastoris, Pichia methanolica y Kluyeromyces lactis. La enzima de alto nivel de rendimeinto y termotolerante fue producida de la fermentación de Pichia Pastoris con codón optimizado de gene fitasa.
MX2011005074A 2008-11-14 2008-11-14 Una fitasa escherichia coli no-k12 termotolerante y su produccion. MX307031B (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2008/073050 WO2010054513A1 (en) 2008-11-14 2008-11-14 A thermotolerant non-k12 escherichia coli phytase and its production

Publications (2)

Publication Number Publication Date
MX2011005074A true MX2011005074A (es) 2011-12-08
MX307031B MX307031B (es) 2012-12-13

Family

ID=42169591

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2011005074A MX307031B (es) 2008-11-14 2008-11-14 Una fitasa escherichia coli no-k12 termotolerante y su produccion.

Country Status (8)

Country Link
US (1) US8652820B2 (es)
EP (1) EP2356237B1 (es)
KR (1) KR101336213B1 (es)
CN (1) CN102209785B (es)
BR (1) BRPI0823236A2 (es)
CA (1) CA2743711C (es)
MX (1) MX307031B (es)
WO (1) WO2010054513A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2617823E (pt) 2006-09-21 2015-11-23 Basf Enzymes Llc Fitases, ácidos nucleicos que as codificam e métodos para as preparar e utilizar
CN101892206A (zh) * 2010-07-27 2010-11-24 温州大学 一种以重组毕赤酵母高效发酵制备植酸酶的工艺
CN102586294B (zh) * 2011-01-12 2013-07-03 上海市农业科学院 一种适于毕赤酵母表达的高比活植酸酶基因及其制备方法和表达
WO2014001349A1 (en) * 2012-06-26 2014-01-03 Dsm Ip Assets B.V. Phytase in biogas production
EP3072962B1 (en) * 2013-11-12 2018-12-26 Feed Research Institute Chinese Academy of Agricultural Sciences A method for producing a phytase variant with improved thermal stability, and a phytase variant and the use thereof
CN105802936B (zh) * 2016-05-26 2019-10-29 湖南南北旺生物技术有限公司 一种耐高温中性植酸酶的制备方法和耐高温中性植酸酶及其应用
CN107353327A (zh) * 2017-03-30 2017-11-17 南京百斯杰生物工程有限公司 植酸酶在黑曲霉中表达
CN115725635A (zh) * 2022-07-28 2023-03-03 青岛蔚蓝生物集团有限公司 一株毕赤酵母突变菌及其在中性植酸酶生产中的应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4824786A (en) 1984-09-14 1989-04-25 The Regents Of The University Of Minnesota Methylotroph cloning vehicle
DE3720834A1 (de) 1987-06-24 1989-01-05 Hoechst Ag Promotoren zur expression von fremd-dna in methylotrophen bakterien, deren gewinnung und deren verwendung
UA27702C2 (uk) 1989-09-27 2000-10-16 Гіст-Брокейдс Н.В. Фрагмент геномної днк, що кодує фітазу aspergillus niger,фрагмент кднк, що кодує фітазу aspergillus niger, рекомбінантна плазмідна днк для експресії фітази в aspergillus (варіанти), штам aspergillus-продуцент фітази aspergillus (варіанти), спосіб одержання фітази, рекомбінанта фітаза aspergillus niger
JPH07506252A (ja) 1992-04-24 1995-07-13 エス・アール・アイ・インターナシヨナル 真核細胞内でのイン・ビボ相同配列ターゲッティング
EP0733059B1 (en) 1993-12-09 2000-09-13 Thomas Jefferson University Compounds and methods for site-directed mutations in eukaryotic cells
US20040091968A1 (en) * 1997-08-13 2004-05-13 Short Jay M. Recombinant phytases and methods of making and using them
US6855365B2 (en) * 1997-08-13 2005-02-15 Diversa Corporation Recombinant bacterial phytases and uses thereof
TWI262083B (en) * 2001-12-28 2006-09-21 Syngenta Participations Ag Microbially-expressed thermotolerant phytase for animal feed
US7026464B2 (en) 2002-10-21 2006-04-11 E. I. Du Pont De Nemours And Company Natural promoters for gene expression in C1 metabolizing bacteria
WO2006063588A1 (en) * 2004-12-13 2006-06-22 Novozymes A/S Polypeptides having acid phosphatase activity and polynucleotides encoding same
CN101260391B (zh) * 2007-03-06 2012-02-22 四川禾本生物工程有限公司 耐热型植酸酶基因及其应用

Also Published As

Publication number Publication date
EP2356237A4 (en) 2012-11-14
BRPI0823236A2 (pt) 2015-06-16
EP2356237A1 (en) 2011-08-17
KR20110086584A (ko) 2011-07-28
CA2743711C (en) 2016-04-05
US20120021488A1 (en) 2012-01-26
EP2356237B1 (en) 2014-07-30
CN102209785B (zh) 2013-04-03
KR101336213B1 (ko) 2013-12-05
CN102209785A (zh) 2011-10-05
US8652820B2 (en) 2014-02-18
MX307031B (es) 2012-12-13
WO2010054513A1 (en) 2010-05-20
CA2743711A1 (en) 2010-05-20

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