MX2011005074A - Una fitasa escherichia coli no-k12 termotolerante y su produccion. - Google Patents
Una fitasa escherichia coli no-k12 termotolerante y su produccion.Info
- Publication number
- MX2011005074A MX2011005074A MX2011005074A MX2011005074A MX2011005074A MX 2011005074 A MX2011005074 A MX 2011005074A MX 2011005074 A MX2011005074 A MX 2011005074A MX 2011005074 A MX2011005074 A MX 2011005074A MX 2011005074 A MX2011005074 A MX 2011005074A
- Authority
- MX
- Mexico
- Prior art keywords
- thermotolerant
- escherichia coli
- production
- phytase gene
- coli phytase
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Esta invención se relaciona con la clonación y secuenciación del gene de fitasa termotolerante de la cepa de Escherichia coli Non-K12, ATCC 9637, expresión del gene de fitasa en el sistema de expresión de la Escherichia coli, uso del codón optimizado y expresión en Pichia Pastoris, Pichia methanolica y Kluyeromyces lactis. La enzima de alto nivel de rendimeinto y termotolerante fue producida de la fermentación de Pichia Pastoris con codón optimizado de gene fitasa.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2008/073050 WO2010054513A1 (en) | 2008-11-14 | 2008-11-14 | A thermotolerant non-k12 escherichia coli phytase and its production |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2011005074A true MX2011005074A (es) | 2011-12-08 |
MX307031B MX307031B (es) | 2012-12-13 |
Family
ID=42169591
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2011005074A MX307031B (es) | 2008-11-14 | 2008-11-14 | Una fitasa escherichia coli no-k12 termotolerante y su produccion. |
Country Status (8)
Country | Link |
---|---|
US (1) | US8652820B2 (es) |
EP (1) | EP2356237B1 (es) |
KR (1) | KR101336213B1 (es) |
CN (1) | CN102209785B (es) |
BR (1) | BRPI0823236A2 (es) |
CA (1) | CA2743711C (es) |
MX (1) | MX307031B (es) |
WO (1) | WO2010054513A1 (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2617814B1 (en) | 2006-09-21 | 2015-11-18 | BASF Enzymes LLC | Phytases, nucleic acids encoding them and methods for making and using them |
CN101892206A (zh) * | 2010-07-27 | 2010-11-24 | 温州大学 | 一种以重组毕赤酵母高效发酵制备植酸酶的工艺 |
CN102586294B (zh) * | 2011-01-12 | 2013-07-03 | 上海市农业科学院 | 一种适于毕赤酵母表达的高比活植酸酶基因及其制备方法和表达 |
US20150337337A1 (en) * | 2012-06-26 | 2015-11-26 | Dsm Ip Assets B.V. | Phytase in biogas production |
WO2015070372A1 (zh) * | 2013-11-12 | 2015-05-21 | 中国农业科学院饲料研究所 | 一种产生热稳定性改良的植酸酶变体的方法、以及植酸酶变体及其应用 |
CN105802936B (zh) * | 2016-05-26 | 2019-10-29 | 湖南南北旺生物技术有限公司 | 一种耐高温中性植酸酶的制备方法和耐高温中性植酸酶及其应用 |
CN107353327A (zh) * | 2017-03-30 | 2017-11-17 | 南京百斯杰生物工程有限公司 | 植酸酶在黑曲霉中表达 |
CN115725635A (zh) * | 2022-07-28 | 2023-03-03 | 青岛蔚蓝生物集团有限公司 | 一株毕赤酵母突变菌及其在中性植酸酶生产中的应用 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4824786A (en) | 1984-09-14 | 1989-04-25 | The Regents Of The University Of Minnesota | Methylotroph cloning vehicle |
DE3720834A1 (de) | 1987-06-24 | 1989-01-05 | Hoechst Ag | Promotoren zur expression von fremd-dna in methylotrophen bakterien, deren gewinnung und deren verwendung |
UA27702C2 (uk) | 1989-09-27 | 2000-10-16 | Гіст-Брокейдс Н.В. | Фрагмент геномної днк, що кодує фітазу aspergillus niger,фрагмент кднк, що кодує фітазу aspergillus niger, рекомбінантна плазмідна днк для експресії фітази в aspergillus (варіанти), штам aspergillus-продуцент фітази aspergillus (варіанти), спосіб одержання фітази, рекомбінанта фітаза aspergillus niger |
AU4115693A (en) | 1992-04-24 | 1993-11-29 | Sri International | In vivo homologous sequence targeting in eukaryotic cells |
CA2178729A1 (en) | 1993-12-09 | 1995-06-15 | Eric B. Kmiec | Compounds and methods for site-directed mutations in eukaryotic cells |
US6855365B2 (en) * | 1997-08-13 | 2005-02-15 | Diversa Corporation | Recombinant bacterial phytases and uses thereof |
US20040091968A1 (en) * | 1997-08-13 | 2004-05-13 | Short Jay M. | Recombinant phytases and methods of making and using them |
TWI262083B (en) * | 2001-12-28 | 2006-09-21 | Syngenta Participations Ag | Microbially-expressed thermotolerant phytase for animal feed |
US7026464B2 (en) | 2002-10-21 | 2006-04-11 | E. I. Du Pont De Nemours And Company | Natural promoters for gene expression in C1 metabolizing bacteria |
WO2006063588A1 (en) * | 2004-12-13 | 2006-06-22 | Novozymes A/S | Polypeptides having acid phosphatase activity and polynucleotides encoding same |
CN101260391B (zh) | 2007-03-06 | 2012-02-22 | 四川禾本生物工程有限公司 | 耐热型植酸酶基因及其应用 |
-
2008
- 2008-11-14 BR BRPI0823236-9A patent/BRPI0823236A2/pt not_active IP Right Cessation
- 2008-11-14 US US13/128,566 patent/US8652820B2/en not_active Expired - Fee Related
- 2008-11-14 CN CN2008801319731A patent/CN102209785B/zh active Active
- 2008-11-14 EP EP08878073.9A patent/EP2356237B1/en not_active Not-in-force
- 2008-11-14 KR KR1020117012872A patent/KR101336213B1/ko active IP Right Grant
- 2008-11-14 MX MX2011005074A patent/MX307031B/es active IP Right Grant
- 2008-11-14 WO PCT/CN2008/073050 patent/WO2010054513A1/en active Application Filing
- 2008-11-14 CA CA2743711A patent/CA2743711C/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
BRPI0823236A2 (pt) | 2015-06-16 |
CA2743711C (en) | 2016-04-05 |
WO2010054513A1 (en) | 2010-05-20 |
US8652820B2 (en) | 2014-02-18 |
MX307031B (es) | 2012-12-13 |
CA2743711A1 (en) | 2010-05-20 |
CN102209785B (zh) | 2013-04-03 |
EP2356237B1 (en) | 2014-07-30 |
KR20110086584A (ko) | 2011-07-28 |
CN102209785A (zh) | 2011-10-05 |
EP2356237A4 (en) | 2012-11-14 |
US20120021488A1 (en) | 2012-01-26 |
EP2356237A1 (en) | 2011-08-17 |
KR101336213B1 (ko) | 2013-12-05 |
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Legal Events
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FG | Grant or registration |