KR20150128705A - 재조합 숙주에서 스테비올 글리코시드의 효율적인 생성 - Google Patents
재조합 숙주에서 스테비올 글리코시드의 효율적인 생성 Download PDFInfo
- Publication number
- KR20150128705A KR20150128705A KR1020157024717A KR20157024717A KR20150128705A KR 20150128705 A KR20150128705 A KR 20150128705A KR 1020157024717 A KR1020157024717 A KR 1020157024717A KR 20157024717 A KR20157024717 A KR 20157024717A KR 20150128705 A KR20150128705 A KR 20150128705A
- Authority
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- South Korea
- Prior art keywords
- gene
- rebaudioside
- steviol
- polypeptide
- transporter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 0 CC(CS(C1)(CC2)C(CC3)C4(C)C2C(C)(**)CCC4)C13C(C)=C Chemical compound CC(CS(C1)(CC2)C(CC3)C4(C)C2C(C)(**)CCC4)C13C(C)=C 0.000 description 6
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
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- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1062—Sucrose synthase (2.4.1.13)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C12P15/00—Preparation of compounds containing at least three condensed carbocyclic rings
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- C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
- C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
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- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
- C12Y114/13078—Ent-kaurene oxidase (1.14.13.78)
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- C12Y204/00—Glycosyltransferases (2.4)
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- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01013—Sucrose synthase (2.4.1.13)
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- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01035—Phenol beta-glucosyltransferase (2.4.1.35)
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- C12Y—ENZYMES
- C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
- C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
- C12Y205/01029—Geranylgeranyl diphosphate synthase (2.5.1.29)
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- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
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- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
- C12Y402/03019—Ent-kaurene synthase (4.2.3.19)
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- C12Y—ENZYMES
- C12Y505/00—Intramolecular lyases (5.5)
- C12Y505/01—Intramolecular lyases (5.5.1)
- C12Y505/01013—Ent-copalyl diphosphate synthase (5.5.1.13)
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- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361763290P | 2013-02-11 | 2013-02-11 | |
| US201361763308P | 2013-02-11 | 2013-02-11 | |
| US61/763,290 | 2013-02-11 | ||
| US61/763,308 | 2013-02-11 | ||
| PCT/EP2014/052675 WO2014122328A1 (en) | 2013-02-11 | 2014-02-11 | Efficient production of steviol glycosides in recombinant hosts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR20150128705A true KR20150128705A (ko) | 2015-11-18 |
Family
ID=50112893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020157024717A Withdrawn KR20150128705A (ko) | 2013-02-11 | 2014-02-11 | 재조합 숙주에서 스테비올 글리코시드의 효율적인 생성 |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US10017804B2 (https=) |
| EP (1) | EP2954061B1 (https=) |
| JP (2) | JP6576247B2 (https=) |
| KR (1) | KR20150128705A (https=) |
| CN (1) | CN105189771B (https=) |
| AU (2) | AU2014213918B2 (https=) |
| BR (1) | BR112015019160A2 (https=) |
| CA (1) | CA2900882A1 (https=) |
| MX (1) | MX2015010098A (https=) |
| MY (1) | MY184253A (https=) |
| SG (2) | SG11201506127WA (https=) |
| WO (1) | WO2014122328A1 (https=) |
Families Citing this family (73)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9107436B2 (en) | 2011-02-17 | 2015-08-18 | Purecircle Sdn Bhd | Glucosylated steviol glycoside as a flavor modifier |
| US10696706B2 (en) | 2010-03-12 | 2020-06-30 | Purecircle Usa Inc. | Methods of preparing steviol glycosides and uses of the same |
| EP2575432B1 (en) | 2010-06-02 | 2019-08-14 | Evolva, Inc. | Recombinant production of steviol glycosides |
| US11690391B2 (en) | 2011-02-17 | 2023-07-04 | Purecircle Sdn Bhd | Glucosylated steviol glycoside as a flavor modifier |
| US9771434B2 (en) | 2011-06-23 | 2017-09-26 | Purecircle Sdn Bhd | Products from stevia rebaudiana |
| CA3128532A1 (en) | 2011-08-08 | 2013-02-14 | Evolva Sa | Recombinant production of steviol glycosides |
| MX395285B (es) | 2012-05-22 | 2025-03-25 | Purecircle Sdn Bhd | Glucosidos de esteviol de alta pureza. |
| US9752174B2 (en) | 2013-05-28 | 2017-09-05 | Purecircle Sdn Bhd | High-purity steviol glycosides |
| MY199452A (en) * | 2013-02-06 | 2023-10-30 | Evolva Sa | Methods for improved production of rebaudioside d and rebaudioside m |
| MX367033B (es) * | 2013-05-31 | 2019-08-02 | Dsm Ip Assets Bv | Microorganismos para la produccion de diterpeno. |
| US10952458B2 (en) | 2013-06-07 | 2021-03-23 | Purecircle Usa Inc | Stevia extract containing selected steviol glycosides as flavor, salty and sweetness profile modifier |
| EP3714699B1 (en) | 2013-06-07 | 2024-12-18 | PureCircle USA Inc. | Stevia extract containing selected steviol glycosides as flavor, salty and sweetness profile modifier |
| EP3021689B1 (en) * | 2013-07-15 | 2021-03-24 | DSM IP Assets B.V. | Diterpene production |
| US10370418B2 (en) * | 2014-05-20 | 2019-08-06 | Lawrence Livermore National Security, Llc | Engineered microorganisms having resistance to ionic liquids |
| CA2957331A1 (en) | 2014-08-11 | 2016-02-18 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| US11202461B2 (en) | 2014-09-02 | 2021-12-21 | Purecircle Sdn Bhd | Stevia extracts |
| CA2960693A1 (en) | 2014-09-09 | 2016-03-17 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| BR112017006163B1 (pt) | 2014-10-03 | 2022-04-05 | Conagen Inc | Produto de consumo oral e método para adoçar o mesmo |
| EP3204506B1 (en) * | 2014-10-08 | 2022-01-12 | DSM IP Assets B.V. | Steviol glycoside production |
| US20170258121A1 (en) * | 2014-11-24 | 2017-09-14 | Cargill, Incorporated | Glycoside compositions |
| CN107249356B (zh) | 2014-12-17 | 2021-08-06 | 嘉吉公司 | 用于口服摄入或使用的甜菊醇糖苷化合物、组合物以及用于增强甜菊醇糖苷溶解度的方法 |
| EP3250686A1 (en) * | 2015-01-30 | 2017-12-06 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| MX2017010311A (es) * | 2015-02-10 | 2017-12-14 | Purecircle Usa Inc | Metodos de produccion y recuperacion de rebaudiosido m biosintetico. |
| WO2016146711A1 (en) | 2015-03-16 | 2016-09-22 | Dsm Ip Assets B.V. | Udp-glycosyltransferases |
| CN107666834B (zh) | 2015-04-03 | 2021-08-24 | 帝斯曼知识产权资产管理有限公司 | 甜菊醇糖苷 |
| CA2985669C (en) | 2015-05-20 | 2022-05-03 | Cargill, Incorporated | Glycoside compositions |
| EP3303552A4 (en) * | 2015-05-29 | 2019-01-16 | Cargill, Incorporated | FERMENTATION METHOD FOR THE PRODUCTION OF STEVIOL GLYCOSIDES WITH MULTIPHASE SUPPLY |
| EP3303553B1 (en) | 2015-05-29 | 2022-07-27 | Cargill, Incorporated | Fermentation methods for producing steviol glycosides using high ph |
| US10612065B2 (en) | 2015-05-29 | 2020-04-07 | Cargill, Incorporated | Heat treatment to produce glycosides |
| CN105154348B (zh) * | 2015-08-05 | 2018-05-04 | 大连理工大学 | 一种提高酿酒酵母对纤维素水解液抑制物耐受性的方法 |
| US10844414B2 (en) | 2015-08-06 | 2020-11-24 | Cargill, Incorporated | Methods for producing steviol glycosides in engineered yeast |
| MX2018001620A (es) | 2015-08-07 | 2018-06-11 | Evolva Sa | Produccion de glucosidos de esteviol en hospedadores recombinantes. |
| CA2993744A1 (en) * | 2015-08-13 | 2017-02-16 | Dsm Ip Assets B.V. | Steviol glycoside transport |
| EP3943504A1 (en) * | 2015-08-13 | 2022-01-26 | DSM IP Assets B.V. | Steviol glycoside transport |
| WO2017034942A1 (en) | 2015-08-21 | 2017-03-02 | Manus Biosynthesis, Inc. | Increasing productivity of e. coli host cells that functionally express p450 enzymes |
| US9765117B2 (en) | 2015-08-24 | 2017-09-19 | Romek Figa | Peptides for treating cancer |
| EP3141610A1 (en) * | 2015-09-12 | 2017-03-15 | Jennewein Biotechnologie GmbH | Production of human milk oligosaccharides in microbial hosts with engineered import / export |
| US11647771B2 (en) | 2015-10-26 | 2023-05-16 | Purecircle Usa Inc. | Steviol glycoside compositions |
| RU2764635C2 (ru) | 2015-12-15 | 2022-01-19 | ПЬЮРСЁРКЛ ЮЭсЭй ИНК. | Композиция стевиол гликозида |
| WO2017153538A1 (en) | 2016-03-11 | 2017-09-14 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| US10982249B2 (en) | 2016-04-13 | 2021-04-20 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| EP3458599A1 (en) * | 2016-05-16 | 2019-03-27 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| JP2019517801A (ja) | 2016-06-15 | 2019-06-27 | コデクシス, インコーポレイテッド | 操作されたβ−グルコシダーゼおよびグルコシル化方法 |
| US20190127772A1 (en) * | 2016-06-15 | 2019-05-02 | Codexis, Inc. | Enzymatic glycosylation of steviol glycosides and other compounds with glucose-1-phosphate |
| WO2017218036A1 (en) * | 2016-06-17 | 2017-12-21 | Cargill, Incorporated | Steviol glycoside compositions for oral ingestion or use |
| PT3497222T (pt) | 2016-08-12 | 2022-02-09 | Amyris Inc | Glicosiltransferase dependente de udp para produção de eficácia elevada de rebaudiosídeos |
| WO2018083338A1 (en) | 2016-11-07 | 2018-05-11 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| CN108085262B (zh) * | 2016-11-23 | 2022-08-05 | 中国科学院天津工业生物技术研究所 | 对萜类或含萜精油耐受性提高或萜类产量提高的重组宿主细胞、其产生方法及其用途 |
| EP3564369A4 (en) | 2016-12-27 | 2020-07-29 | Suntory Holdings Limited | STEVIOL GLYCOSIDE HEXOSE TRANSFERASE AND CODING GENE FOR THIS |
| BR112019015454A2 (pt) | 2017-01-26 | 2020-03-24 | Manus Bio, Inc. | Manipulação metabólica para produção microbiana de produtos terpenoides |
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| KR101559478B1 (ko) | 2013-06-24 | 2015-10-13 | 한국생명공학연구원 | 효소전환법을 이용한 천연 고감미료의 제조방법 |
| EP3021689B1 (en) | 2013-07-15 | 2021-03-24 | DSM IP Assets B.V. | Diterpene production |
| EP3024941B1 (en) | 2013-07-23 | 2019-07-03 | DSM IP Assets B.V. | Diterpene production in yarrowia |
| AU2014298430A1 (en) | 2013-07-31 | 2016-02-11 | Dsm Ip Assets B.V. | Steviol glycosides |
| EP3536697A1 (en) | 2013-07-31 | 2019-09-11 | DSM IP Assets B.V. | Recovery of steviol glycosides |
| WO2015016393A1 (ja) | 2013-08-02 | 2015-02-05 | サントリーホールディングス株式会社 | ヘキセノール配糖体化酵素の利用方法 |
| CN103397064B (zh) | 2013-08-14 | 2015-04-15 | 苏州汉酶生物技术有限公司 | 一种酶法制备瑞鲍迪甙m的方法 |
| US20160215306A1 (en) | 2013-08-30 | 2016-07-28 | Evolva Sa | Method for producing modified resveratrol |
| SG10201807693YA (en) | 2014-03-07 | 2018-10-30 | Evolva Sa | Methods for recombinant production of saffron compounds |
| CA2957331A1 (en) | 2014-08-11 | 2016-02-18 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| CA2960693A1 (en) | 2014-09-09 | 2016-03-17 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| EP3250686A1 (en) | 2015-01-30 | 2017-12-06 | Evolva SA | Production of steviol glycosides in recombinant hosts |
| CN104845990A (zh) | 2015-06-11 | 2015-08-19 | 山东大学 | 拟南芥糖基转移酶基因ugt73c7在提高植物抗病性中的应用 |
| MX2018001620A (es) | 2015-08-07 | 2018-06-11 | Evolva Sa | Produccion de glucosidos de esteviol en hospedadores recombinantes. |
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2014
- 2014-02-11 BR BR112015019160A patent/BR112015019160A2/pt not_active Application Discontinuation
- 2014-02-11 JP JP2015556536A patent/JP6576247B2/ja not_active Expired - Fee Related
- 2014-02-11 MX MX2015010098A patent/MX2015010098A/es unknown
- 2014-02-11 CA CA2900882A patent/CA2900882A1/en not_active Abandoned
- 2014-02-11 SG SG11201506127WA patent/SG11201506127WA/en unknown
- 2014-02-11 EP EP14704558.7A patent/EP2954061B1/en active Active
- 2014-02-11 CN CN201480008381.6A patent/CN105189771B/zh active Active
- 2014-02-11 KR KR1020157024717A patent/KR20150128705A/ko not_active Withdrawn
- 2014-02-11 MY MYPI2015001996A patent/MY184253A/en unknown
- 2014-02-11 AU AU2014213918A patent/AU2014213918B2/en active Active
- 2014-02-11 SG SG10201705993YA patent/SG10201705993YA/en unknown
- 2014-02-11 WO PCT/EP2014/052675 patent/WO2014122328A1/en not_active Ceased
- 2014-02-11 US US14/764,898 patent/US10017804B2/en active Active
-
2018
- 2018-02-08 AU AU2018200950A patent/AU2018200950A1/en not_active Abandoned
- 2018-06-07 US US16/002,245 patent/US11021727B2/en active Active
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2019
- 2019-04-05 JP JP2019072616A patent/JP2019162110A/ja not_active Withdrawn
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2021
- 2021-04-29 US US17/244,089 patent/US20220017932A1/en not_active Abandoned
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|---|---|
| US20180371517A1 (en) | 2018-12-27 |
| CN105189771B (zh) | 2019-11-05 |
| CA2900882A1 (en) | 2014-08-14 |
| US20220017932A1 (en) | 2022-01-20 |
| US11021727B2 (en) | 2021-06-01 |
| SG10201705993YA (en) | 2017-08-30 |
| MY184253A (en) | 2021-03-29 |
| US10017804B2 (en) | 2018-07-10 |
| BR112015019160A2 (pt) | 2017-08-22 |
| MX2015010098A (es) | 2016-04-19 |
| JP2019162110A (ja) | 2019-09-26 |
| JP6576247B2 (ja) | 2019-09-18 |
| WO2014122328A1 (en) | 2014-08-14 |
| EP2954061B1 (en) | 2023-11-22 |
| CN105189771A (zh) | 2015-12-23 |
| AU2018200950A1 (en) | 2018-03-01 |
| AU2014213918A1 (en) | 2015-08-20 |
| SG11201506127WA (en) | 2015-09-29 |
| EP2954061A1 (en) | 2015-12-16 |
| US20150361476A1 (en) | 2015-12-17 |
| AU2014213918B2 (en) | 2017-11-09 |
| WO2014122328A8 (en) | 2015-09-17 |
| JP2016506743A (ja) | 2016-03-07 |
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