AU2018200459A1 - Recombinant production of steviol glycosides - Google Patents

Abstract

RECOMBINANT PRODUCTION OF STEVIOL GLYCOSIDES Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements.

Description

TECHNICAL FIELD

This disclosure relates to the recombinant production of steviol and steviol glycosides. In particular, this disclosure relates to the production of steviol and steviol glycosides such as rubusoside and/or rebaudioside A by recombinant hosts such as recombinant microorganisms, plants, or plant cells. This disclosure also provides compositions containing steviol glycosides.

BACKGROUND

Sweeteners are well known as ingredients used most commonly in the food, beverage, or confectionary industries. The sweetener can either be incorporated into a final food product during production or for stand-alone use, when appropriately diluted, as a tabletop sweetener or an at-home replacement for sugars in baking. Sweeteners include natural sweeteners such as sucrose, high fructose corn syrup, molasses, maple syrup, and honey and artificial sweeteners such as aspartame, saccharine and sucralose. Stevia extract is a natural sweetener that can be isolated and extracted from a perennial shrub, Stevia rebaudiana. Stevia is commonly grown in South America and Asia for commercial production of stevia extract. Stevia extract, purified to various degrees, is used commercially as a high intensity sweetener in foods and in blends or alone as a tabletop sweetener.

(14166224_1):GGG

Extracts of the Stevia plant contain rebaudiosides and other steviol glycosides that contribute to the sweet flavor, although the amount of each glycoside often varies among different production batches. Existing commercial products are predominantly rebaudioside

A with lesser amounts of other glycosides such as rebaudioside C, D, and F. Stevia extracts may also contain contaminants such as plant-derived la

2018200459 19 Jan 2018 (14166224_1):GGG

2018200459 19 Jan 2018 compounds that contribute to off-flavors. These off-flavors can be more or less problematic depending on the food system or application of choice. Potential contaminants include pigments, lipids, proteins, phenolics, saccharides, spathulenol and other sesquiterpenes, labdane diterpenes, monoterpenes, decanoic acid, 8,11,145 eicosatrienoic acid, 2-methyloctadecane, pentacosane, octacosane, tetracosane, octadecanol, stigmasterol, β-sitosterol, a- and β-amyrin, lupeol, β-amryin acetate, pentacyclic triterpene, centauredin, quercitin, epi-alpha-cadinol, carophyllenes and derivatives, beta-pinene, beta-sitosterol, and gibberellin.

SUMMARY o Provided herein is a recombinant host, such as a microorganism, comprising one or more biosynthesis genes whose expression results in production of steviol.

Such genes include a gene encoding a copalyl diphosphate synthase, a gene encoding a kaurene synthase, a gene encoding a kaurene oxidase; and a gene encoding a steviol synthetase. The recombinant host can include a gene encoding a bifunctional copalyl diphosphate synthase and kaurene synthase, in place of the genes encoding copalyl diphosphate synthase and kaurene synthase. At least one of the genes is a recombinant gene. In some embodiments the recombinant host further comprises a gene encoding a geranylgeranyl diphosphate synthase. The recombinant host can further comprise a gene encoding a truncated HMG-CoA reductase and/or a gene encoding a CPR. The expression of one or more of the genes can be inducible.

In one aspect, this document features a recombinant host that includes a recombinant gene encoding a UGT91D2 polypeptide (e.g., a UGT91D2e or UGT91D2m polypeptide). The UGT91D2 polypeptide can have at least 90% identity (e.g., at least 95% or 99% identity) to the amino acid sequence set forth in SEQ ID

NO:5. The UGT91D2 polypeptide can include at least one amino acid substitution at residues 1-19, 27-38, 44-87, 96-120, 125-141, 159-184, 199-202, 215-380, or 387-473 of SEQ ID NO:5. For example, the UGT91D2 polypeptide can include an amino acid substitution at one or more residues selected from the group consisting of residues 30, 93, 99, 122, 140, 142, 148, 153, 156, 195, 196, 199, 206, 207, 211, 221, 286, 343,

427, and 438 of SEQ ID NO:5. In one embodiment, the UGT91D2 polypeptide includes an arginine at residue 206, a cysteine at residue 207, and an arginine at

2018200459 19 Jan 2018 residue 343 relative to SEQ ID NOG. In one embodiment, the UGT91D2 polypeptide includes a phenylalanine at residue 30, a glutamine at residue 93, a valine at residue

99, a phenylalanine at residue 122, a tyrosine at residue 140, a cysteine at residue 142, a threonine at residue 148, an alanine at residue 153, a serine at residue 156, a methionine at residue 195, a glutamic acid at residue 196, a glutamic acid at residue 199, a methionine at residue 211, a phenylalanine at residue 221, an alanine at residue 286, an asparagine at residue 427, or an alanine at residue 438 relative to SEQ ID NOG. The polypeptide can have the amino acid sequence of SEQ ID NOG or SEQ ID NO:95.

o A host described herein further can include a recombinant gene encoding a

UGT85C polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NOG. For example, the UGT85C polypeptide can include one or more amino acid substitutions at residues 9, 10, 13, 15, 21, 27, 60, 65, 71, 87, 91, 220, 243, 270, 289, 298, 334, 336, 350, 368, 389, 394, 397, 418, 420, 440, 441, 444, and

471 of SEQ ID NOG.

A host described herein further can include a recombinant gene encoding a UGT76G polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NOG. For example, the UGT76G polypeptide can have one or more amino acid substitutions at residues 29, 74, 87, 91, 116, 123, 125, 126, 130, 145, 192,

1 93, 194, 196, 198, 199, 200, 203, 204, 205, 206, 207, 208, 266, 273, 274, 284, 285,

291, 330, 331, and 346 of SEQ ID NOG.

This document also features a recombinant host that includes a recombinant gene encoding a UGT85C polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NOG, and having one or more amino acid substitutions at residues 9, 10, 13, 15, 21, 27, 60, 65, 71, 87, 91, 220, 243, 270, 289, 298, 334, 336, 350, 368, 389, 394, 397, 418, 420, 440, 441, 444, and 471 of SEQ ID NOG. For example, the UGT85C polypeptide can include substitutions at residues 13, 15, 60, 270, 289, and 418 of SEQ ID NOG. For example, the UGT85C polypeptide can include a) substitutions at residues 13, 60, and 270 of SEQ ID NOG; b) substitutions at residues 60 and 87 of SEQ ID NOG; c) substitutions at residues 65, 71, 220, 243, and 270 of SEQ ID NOG; d) substitutions at residues 65, 71, 220, 243, 270, and 441 of SEQ ID NOG; e) substitutions at residues 65, 71, 220, 389, and 394 of SEQ ID

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NO:3; f) substitutions at residues 65, 71, 270, and 289 of SEQ ID NO:3; g) substitutions at residues 15 and 65 of SEQ ID NO:3; h) substitutions at residues 65 and 270 of SEQ ID NO:3; i) substitutions at residues 65 and 440 of SEQ ID NO:3; j) substitutions at residues 65 and 441 of SEQ ID NO:3; k) substitutions at residues 65 and 418 of SEQ ID NO:3; 1) substitutions at residues 220, 243, 270, and 334 of SEQ ID NO:3; or m) substitutions at residues 270 and 289 of SEQ ID NO:3.

In another aspect, this document features a recombinant host that includes a recombinant gene encoding a UGT76G polypeptide having at least 90% identity to the amino acid sequence set forth in SEQ ID NO:7, and having one or more amino acid substitutions at residues 29, 74, 87, 91, 116, 123, 125, 126, 130, 145, 192, 193, 194, 196, 198, 199, 200, 203, 204, 205, 206, 207, 208, 266, 273, 274, 284, 285, 291, 330, 331, and 346. For example, the UGT76G polypeptide can have a) substitutions at amino acid residues 74,87, 91, 116, 123, 125, 126, 130, 145, 192, 193, 194, 196, 198, 199, 200, 203, 204, 205, 206, 207, 208, and 291; b) substitutions at residues 74 ,

87, 91, 116, 123, 125, 126, 130, 145, 192, 193, 194, 196, 198, 199, 200, 203, 204,

205, 206, 207, 208, 266, 273, 274, 284, 285, and 291; or c) substitutions at residues 74,87, 91, 116, 123, 125, 126, 130, 145, 192, 193, 194, 196, 198, 199, 200, 203, 204, 205, 206, 207, 208, 266, 273, 274, 284, 285, 291, 330, 331, and 346.

Any of the hosts described herein further can include a gene encoding a

UGT74G1 polypeptide (e.g., a recombinant gene encoding a UGT74G1 polypeptide).

Any of the hosts described herein further can include one or more of: (i) a gene encoding a geranylgeranyl diphosphate synthase; (ii) a gene encoding a bifunctional copalyl diphosphate synthase and kaurene synthase, or a gene encoding a copalyl diphosphate synthase and a gene encoding a kaurene synthase; (iii) a gene encoding a kaurene oxidase; (iv) a gene encoding a steviol synthetase; (v) a gene encoding a truncated HMG-CoA; (vi) a gene encoding a CPR; (vii) a gene encoding a rhamnose synthetase; (viii) a gene encoding a UDP-glucose dehydrogenase; and (ix) a gene encoding a UDP-glucuronic acid decarboxylase. At least one of the genes of (i), (ii), (iii), (iv), (v), (vi), (vii), (viii), or (ix) can be a recombinant gene. In some embodiments, each of the genes of (i), (ii), (iii), and (iv) is a recombinant gene.

This document also features an isolated nucleic acid encoding a polypeptide having at least 90% sequence identity (e.g., at least 95% or 99% sequence identity) to

2018200459 19 Jan 2018 the amino acid sequence set forth in SEQ ID NO:5. The polypeptide can include at least one amino acid substitution at residues 1-19, 27-38, 44-87, 96-120, 125-141,

159-184, 199-202,215-380, or 387-473 ofSEQIDNO:5. The polypeptide can include an amino acid substitution at one or more residues selected from the group consisting of residues 30, 93, 99, 122, 140, 142, 148, 153, 156, 195, 196, 199, 206, 207, 211, 221, 286, 343, 427, and 438 of SEQ ID NO:5. The polypeptide can include an arginine at residue 206, a cysteine at residue 207, and an arginine at residue 343 of SEQ ID NO:5. In some embodiments, the polypeptide includes a phenylalanine at residue 30, a glutamine at residue 93, a valine at residue 99, a phenylalanine at residue

122, a tyrosine at residue 140, a cysteine at residue 142, a threonine at residue 148, an alanine at residue 153, a serine at residue 156, a methionine at residue 195, a glutamic acid at residue 196, a glutamic acid at residue 199, a methionine at residue 211, a phenylalanine at residue 221, an alanine at residue 286, an asparagine at residue 427, or an alanine at residue 438 of SEQ ID NO:5.

In another aspect, this document features an isolated polypeptide having an amino acid sequence with at least 90% identity to the amino acid sequence of SEQ ID NO:5.

This document also features a recombinant host that includes (i) a gene encoding a geranylgeranyl diphosphate synthase; (ii) a gene encoding a bifunctional copalyl diphosphate synthase and kaurene synthase, or a gene encoding a copalyl diphosphate synthase and a gene encoding a kaurene synthase; (iii) a gene encoding a kaurene oxidase; and (iv) a gene encoding a steviol synthetase; wherein at least one of said genes. The host can produce steviol when cultured under conditions in which each of the genes is expressed, and can accumulate to at least 1 mg/L in the culture medium. The geranylgeranyl diphosphate synthase can have greater than 90 % sequence identity to one of the amino acid sequences set forth in SEQ ID NOs: 121128. The copalyl diphosphate synthase can have greater than 90 % sequence identity to one of the amino acid sequences set forth in SEQ ID NOs:129-131. The kaurene synthase can have greater than 90 % sequence identity to one of the amino acid sequences set forth in 132-135. The kaurene oxidase can have greater than 90 % sequence identity to one of the amino acid sequences set forth in 138-141. The steviol synthetase can have greater than 90 % sequence identity to one of the amino acid

2018200459 19 Jan 2018 sequences set forth in SEQ ID NOs: 142-146. The host further can include a gene encoding a truncated HMG-CoA and/or a gene encoding a CPR.

Any of the recombinant hosts further can include one or more of a gene encoding a UGT74G1 polypeptide, a UGT85C2 polypeptide, a UGT76G1 polypeptide, or a UGT91D2 polypeptide.

Any of the recombinant hosts can produce at least one steviol glycoside when cultured under conditions in which each of the genes is expressed. The steviol glycoside can be selected from the group consisting of steviol-13-O-glucoside, steviol-19-O-glucoside, rubusoside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, rebaudioside F, and dulcoside A. The steviol glycoside can accumulate to at least 1 mg/liter (e.g., at least 10 mg/liter or 20 mg/liter) of culture medium when cultured under said conditions.

Any of the recombinant hosts further can include one or more of i) a gene encoding a deoxyxylulose 5-phosphate synthase (DXS); ii) a gene encoding a D-l15 deoxyxylulose 5-phosphate reductoisomerase (DXR); iii) a gene encoding a 4diphosphocytidyl-2-C-methyl-D-erythritol synthase (CMS); iv) a gene encoding a 4diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK); v) a gene encoding a 4diphosphocytidyl-2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MCS); vi) a gene encoding a l-hydroxy-2-methyl-2(E)-butenyl 4-diphosphate synthase (HDS);

or vii) a gene encoding a l-hydroxy-2-methyl-2(E)-butenyl 4-diphosphate reductase (HDR).

Any of the recombinant hosts further can include one or more of ix) a gene encoding a acetoacetyl-CoA thiolase; x) a gene encoding a truncated HMG-CoA reductase; xi) a gene encoding a mevalonate kinase; xii) a gene encoding a phosphomevalonate kinase; or xiii) a gene encoding a mevalonate pyrophosphate decarboxylase.

In any of the hosts described herein, expression of one or more of the genes can be inducible.

Any of the hosts described herein can be a microorganism (e.g., a 30 Saccharomycete such as Saccharomyces cerevisiae, or Escherichia coli), or a plant or plant cell (e.g., a Stevia such as a Stevia rebaudiana, Physcomitrella, or tobacco plant or plant cell).

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In another aspect, this document features a method of producing steviol or a steviol glycoside. The method includes growing a host described herein in a culture medium, under conditions in which the genes are expressed; and recovering the steviol or steviol glycoside produced by the host. The growing step can include inducing expression of one or more of the genes. The steviol or steviol glycoside is selected from the group consisting of steviol-13-O-glucoside, steviol-19-O-glucoside, rubusoside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, rebaudioside F, and dulcoside A.

Also provided herein is a method of producing steviol or a steviol glycoside.

o The method includes growing a microorganism in a culture medium, under conditions in which a geranylgeranyl diphosphate synthase, copalyl diphosphate synthase, kaurene synthase, kaurene oxidase, kaurenoic acid 13-hydroxylase gene and optionally a UGT74G1 and/or a UGT85C2 gene are expressed, and recovering the steviol or steviol glycoside produced by the microorganism. The microorganism can be a Saccharomyces spp. In some embodiments, the growing step comprises inducing expression of one or more of the geranylgeranyl diphosphate synthase, copalyl diphosphate synthase, kaurene synthase, kaurene oxidase, kaurenoic acid 13hydroxylase, UGT74G1 and UGT85C2 genes. In some embodiments, the recovering step comprises purifying the steviol or steviol glycoside from the culture medium by

HPLC. The steviol or steviol glycoside can be steviol, rubusoside, rebaudioside C, rebaudioside F, or dulcoside A.

Also provided herein is a recombinant Saccharomyces strain, comprising one or more biosynthesis genes whose expression results in production of ent-kaurene. The biosynthesis genes include a gene encoding a bifunctional copalyl diphosphate synthase and kaurene synthase, or a gene encoding a copalyl diphosphate synthase and a gene encoding a kaurene synthase. The strain produces ent-kaurene upon expression of the copalyl diphosphate synthase and the kaurene synthase.

In another aspect, this document features an isolated nucleic acid having greater than 90 % sequence identity (e.g., greater than 95% or 99% sequence identity) to one of the nucleotide sequences set forth in SEQ ID NOs: 18-25, 34-36, 4-43, 48, 49, 52-55, 60-64, 70-72, 77, or 79.

2018200459 19 Jan 2018

This document also features a recombinant host that includes (i) a gene encoding a UGT74G1; (ii) a gene encoding a UGT85C2; (iii) a gene encoding a

UGT76G1; and (iv) a gene encoding a UGT91D2, wherein at least one of said genes is a recombinant gene. In some embodiments, each of the genes is a recombinant gene. The host can produce at least one steviol glycoside when cultured under conditions in which each of the genes is expressed. The host further can include (a) a gene encoding a bifunctional copalyl diphosphate synthase and kaurene synthase, or a gene encoding a copalyl diphosphate synthase and a gene encoding a kaurene synthase; (b) a gene encoding a kaurene oxidase; (c) a gene encoding a steviol synthetase; and (d) a gene encoding a geranylgeranyl diphosphate synthase. The steviol glycoside can be rebaudioside A, rebaudioside D or rebaudioside E. This document also features a steviol glycoside composition produced by such a host. The composition can have greater than 4% rebaudioside D by weight of total steviol glycosides and a reduced level of stevia plant-derived contaminants relative to a stevia extract. The composition can have greater than 4% rebaudioside E by weight of total steviol glycosides and a reduced level of stevia plant-derived contaminants relative to a stevia extract.

Also featured herein is an isolated nucleic acid encoding a polypeptide having greater than 90 % sequence identity to the amino acid sequences of UGT91D2e and

UGT91D2m, excluding the amino acid sequence of UGT91D2m, as well as the isolated polypeptides having greater than 90 % sequence identity to the amino acid sequence of UGT91D2e or UGT91D2m, excluding the amino acid sequence of UGT91D2m.

This document also features steviol glycoside composition produced by the host described herein. The composition having reduced levels of stevia plant-derived contaminants relative to a stevia extract.

In another aspect, this document features a recombinant host. The host includes (i) a recombinant gene encoding a UGT91D2; (ii) a recombinant gene encoding a UGT74G1; (iii) a recombinant gene encoding a UGT85C2; (iv) a recombinant gene encoding a UGT76G1; and (v) a gene encoding a rhamnose synthetase, wherein the host produces at least one steviol glycoside when cultured under conditions in which each of the genes is expressed. The host further can

2018200459 19 Jan 2018 include (a) a gene encoding a bifunctional copalyl diphosphate synthase and kaurene synthase, or a gene encoding a copalyl diphosphate synthase and a gene encoding a kaurene synthase; (b) a gene encoding a kaurene oxidase; (c) a gene encoding a steviol synthetase; and (d) a gene encoding a geranylgeranyl diphosphate synthase.

The steviol glycoside can be rebaudioside C or dulcoside A. This document also features a steviol glycoside composition produced by such a host. The composition has greater than 15% rebaudioside C by weight of total steviol glycosides and a reduced level of stevia plant-derived contaminants relative to a stevia extract. A steviol glycoside composition produced by such a host also is featured. The o composition can have greater than 15% dulcoside A by weight of total steviol glycosides and a reduced level of stevia plant-derived contaminants relative to a stevia extract.

This document also features a recombinant host. The host includes (i) a recombinant gene encoding a UGT91D2; (ii) a recombinant gene encoding a

UGT74G1; (iii) a recombinant gene encoding a UGT85C2; (iv) a recombinant gene encoding a UGT76G1; (v) a gene encoding a UDP-glucose dehydrogenase; and (vi) a gene encoding a UDP-glucuronic acid decarboxylase, wherein the host produces at least one steviol glycoside when cultured under conditions in which each of the genes is expressed. The host further can include (a) a gene encoding a bifunctional copalyl diphosphate synthase and kaurene synthase, or a gene encoding a copalyl diphosphate synthase and a gene encoding a kaurene synthase; (b) a gene encoding a kaurene oxidase; (c) a gene encoding a steviol synthetase; and (d) a gene encoding a geranylgeranyl diphosphate synthase. The steviol glycoside can be rebaudioside F. This document also features a steviol glycoside composition produced by such hosts.

The composition can have greater than 4% rebaudioside F by weight of total steviol glycosides and a reduced level of stevia plant-derived contaminants relative to a stevia extract.

In another aspect, this document features a method of producing a steviol glycoside composition. The method includes growing a host described herein in a culture medium, under conditions in which each of the genes is expressed; and recovering the steviol glycoside composition produced by the host, wherein the recovered composition is enriched for rebaudioside A, rebaudioside C, rebaudioside

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D, rebaudioside E, rebaudioside F or dulcoside A relative to the steviol glycoside composition of a wild-type Stevia plant. The steviol glycoside composition produced by the host (e.g., microorganism) can have a reduced level of stevia plant-derived contaminants relative to a stevia extract.

This document also features a food product that includes a steviol glycoside composition enriched for rebaudioside A, rebaudioside C, rebaudioside D, rebaudioside E, rebaudioside F or dulcoside A relative to the steviol glycoside composition of a wild-type Stevia plant.

In another aspect, this document features a method of identifying whether a polymorphism is associated with variation in a trait. The method includes determining whether one or more genetic polymorphisms in a population of plants is associated with the locus for a polypeptide set forth in SEQ ID NO :5 and functional homologs thereof; and measuring the correlation between variation in the trait in plants of the population and the presence of the one or more genetic polymorphisms in plants of the population, thereby identifying whether or not the one or more genetic polymorphisms are associated with variation in the trait.

In yet another aspect, this document features a mehod of making a plant line. The method includes determining whether one or more genetic polymorphisms in a population of plants is associated with the locus for a polypeptide set forth in SEQ ID

NO:5 and functional homologs thereof; identifying one or more plants in the population in which the presence of at least one of the genetic polymorphisms is associated with variation in a trait; crossing one or more of the identified plants with itself or a different plant to produce seed; crossing at least one progeny plant grown from the seed with itself or a different plant; and repeating the crossing steps for an additional 0-5 generations to make said plant line, wherein at least one of the genetic polymorphisms is present in the plant line.

This document also features a method for transferring a second sugar moiety to the C-2' of a glucose in a steviol glycoside. The method includes contacting the steviol glycoside with a UGT91D2 polypeptide and a UDP-sugar under suitable reaction conditions for the transfer of the second sugar moiety to the steviol glycoside. The UGT91D2 polypeptide can have at least 90% sequence identity (e.g., at least 95%

2018200459 19 Jan 2018 or 99%) to the amino acid sequence set forth in SEQ ID NO:5. The UGT91D2 polypeptide can inlcude at least one amino acid substitution at residues 1-19, 27-38,

44-87, 96-120, 125-141, 159-184, 199-202,215-380, or 387-473 ofSEQIDNO:5.

The UGT91D2 polypeptide can include an amino acid substitution at one or more residues selected from the group consisting of residues 30, 93, 99, 122, 140, 142, 148, 153, 156, 195, 196, 199, 206, 207,211,221,286, 343, 427, and 438 of SEQ IDNO:5. The steviol glycoside can be selected from the group consisting of steviol-13-0glucoside, rubusoside, stevioside, and Rebaudioside A. The steviol glycoside can be rubusoside and the second sugar moiety is glucose, and stevioside is produced upon transfer of the second glucose moiety. The steviol glycoside can be stevioside and the second sugar moiety can be glucose, and Rebaudioside E is produced upon transfer of the second glucose moiety. The steviol glycoside can be stevioside, wherein stevioside is contacted with the UGT91D2 polypeptide and a UGT76G1 polypeptide under suitable reaction conditions to produce Rebaudioside D. The steviol glycoside can be steviol-13-0-glucoside and steviol-1,2 bioside is produced upon transfer of said second glucose moiety. The steviol glycoside can be steviol-13-0-glucoside and steviol-1,2-xylobioside is produced upon transfer of the second sugar moiety. The steviol glycoside can be steviol-13-O-glucoside and steviol-1,2-rhamnobioside can be produced upon transfer of the second sugar moiety. The steviol glycoside can be

Rebaudioside A, and Rebaudioside D is produced upon transfer of a second glucose moiety.

In another aspect, this document features a method of determining the presence of a polynucleotide in a Stevia plant. The method includes contacting at least one probe or primer pair with nucleic acid from the Stevia plant, wherein the probe or primer pair is specific for a polynucleotide that encodes a UGT polypeptide, wherein the UGT polypeptide has at least 90% sequence identity to SEQ ID NO: 5, SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO:7 and determining whether or not the polynucleotide is present in said Stevia plant.

This document also features a kit for genotyping a Stevia biological sample.

The kit includes a primer pair that specifically amplifies, or a probe that specifically hybridizes to, a polynucleotide that encodes a UGT polypeptide having at least 90% sequence identity to SEQ ID NO: 5, SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO:7.

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Also provided herein is a recombinant microorganism, comprising one or more biosynthesis genes whose expression results in production of one or more steviol glycosides. The biosynthesis genes include a gene encoding a geranylgeranyl diphosphate synthase, a gene encoding a copalyl diphosphate synthase and a gene encoding a kaurene synthase, a gene encoding a kaurene oxidase, a gene encoding a steviol synthetase, and a gene encoding a UGT74G1 and/or a UGT85C2. At least one of the genes is a recombinant gene. The microorganism can comprise a gene encoding a bifunctional copalyl diphosphate synthase and kaurene synthase in place of the genes encoding copalyl diphosphate synthase and kaurene synthase.

The recombinant microorganism produces at least one steviol glycoside when cultured under conditions in which each of the genes is expressed. The steviol glycoside can be rubusoside, rebaudioside C, rebaudioside F, dulcoside B, or dulcoside A.

The recombinant microorganism can be a Saccharomycete, e.g.,

Saccharomyces cerevisiae, and can have one or more genetic modifications that reduce EXG1 and EXG2 glycoside hydrolase activity relative to a control microorganism that lacks such genetic modifications, and can have one or more genetic modifications that reduce ergosterol biosynthesis relative to a control microorganism that lacks such genetic modifications. The Saccharomycete produces rubusoside when cultured under conditions in which each of the genes is expressed. The rubusoside can accumulate to at least 10 mg/liter of culture medium. The Saccharomycete can be a Saccharomyces cerevisiae strain designated CEY171, CEY191,or CEY213.

The recombinant microorganism can further comprise a gene encoding an

SM12UGT and a gene encoding a UGT76G1, and produce a steviol glycoside when cultured under conditions in which each of the genes is expressed. The steviol glycoside can be rebaudioside A.

Also provided herein is a recombinant microorganism, comprising one or more biosynthesis genes whose expression results in production of at least one steviol glycoside. The biosynthesis genes include a gene encoding an SM12UGT, a gene encoding a UGT74G1, a gene encoding a UGT76G1 and a gene encoding a UGT85C2. The recombinant microorganism produces rebaudioside A or rebaudioside

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B when cultured under conditions in which each of the genes is expressed. The rebaudioside A or rebaudioside B can accumulate to at least 1 mg/L in the culture medium.

Also featured herein is a recombinant microorganism, comprising a gene encoding a UGT91D2 polypeptide, e.g., a recombinant UGT91D2 gene.

Also featured herein is a recombinant microorganism, comprising a gene encoding a geranylgeranyl diphosphate synthase, a gene encoding a bifunctional copalyl diphosphate synthase and kaurene synthase (or a gene encoding a copalyl diphosphate synthase and a gene encoding a kaurene synthase), a gene encoding a kaurene oxidase, a gene encoding a steviol synthetase, a gene encoding a UGT74G1, a gene encoding a UGT85C2, a gene encoding a UGT76G1, and a gene encoding a UGT91D2. At least one of the genes is a recombinant gene. The recombinant microorganism can produce at least one steviol glycoside, e.g., rebaudioside A, rebaudioside B, and/or rebaudioside F, when cultured under conditions in which each of the genes is expressed. The recombinant microorganism can accumulate at least 20 mg of steviol glycoside per liter of culture medium when cultured under such conditions. The recombinant microorganism can be a Saccharomycete, e.g., Saccharomyces cerevisiae, and can have one or more genetic modifications that reduce EXG1 and EXG2 glycoside hydrolase activity relative to a control microorganism that lacks such genetic modifications, and can have one or more genetic modifications that reduce ergosterol biosynthesis relative to a control microorganism that lacks such genetic modifications.

Also featured herein is a recombinant microorganism, comprising a gene encoding a UGT74G1, a gene encoding a UGT85C2, a gene encoding a UGT76G1, and a gene encoding a UGT91D2. At least one of the genes is a recombinant gene.

The recombinant microorganism can produce a steviol glycoside, e.g., rebaudioside A or rebaudioside B, when cultured under conditions in which each of the genes is expressed. The rebaudioside A or rebaudioside B can accumulate to at least 15 mg/L in the culture medium.

The recombinant microorganisms described above can further comprise a gene encoding a deoxyxylulose 5-phosphate synthase (DXS), and/or a gene encoding a ΟΙ-deoxyxylulose 5-phosphate reductoisomerase (DXR), and/or a gene encoding a 413

2018200459 19 Jan 2018 diphosphocytidyl-2-C-methyl-D-erythritol synthase (CMS), and/or a gene encoding a

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK), and/or a gene encoding a

4-diphosphocytidyl-2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MCS), and/or a gene encoding a l-hydroxy-2-methyl-2(E)-butenyl 4-diphosphate synthase (HDS), and/or a gene encoding a 1-hydroxy-2-methyl-2(E)-butenyl 4-diphosphate reductase (HDR).

The recombinant microorganisms described above can further comprise a gene encoding a acetoacetyl-CoA thiolase, and/or a gene encoding a truncated HMG-CoA reductase, and/or a gene encoding a mevalonate kinase, and/or a gene encoding a phosphomevalonate kinase, and/or a gene encoding a mevalonate pyrophosphate decarboxylase.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention belongs. Although methods and materials similar or equivalent to those described herein can be used to practice the invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and are not intended to be limiting. Other features and advantages of the invention will be apparent from the following detailed description. Applicants reserve the right to alternatively claim any disclosed invention using the transitional phrase “comprising,” “consisting essentially of,” or “consisting of,” according to standard practice in patent law.

DESCRIPTION OF DRAWINGS

FIG. 1 is a scheme illustrating the biosynthesis of steviol from geranylgeranyl diphosphate.

FIGS. 2A- D show representative pathways for the biosynthesis of steviol glycosides from steviol.

FIG 3 shows chemical structures for various steviol glycosides.

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FIG. 4 is a schematic representation of rebA production in Saccharomyces cerevisiae.

FIG. 5 is a schematic representation of the concatenation of genes to form eYACs.

FIG. 6 shows rubusoside production by yeast strain CEY13 under various culture conditions.

FIG. 7 shows data obtained from *H and ¹³C NMR analysis of the compound produced by yeast strain CEY213, compared to literature values for rubusoside.

FIG. 8 is an alignment of UGT91D1 and UGT91D2 amino acid sequences (SEQ ID NOs: 14, 16, 12, 5, and 10, respectively).

FIG. 9 shows Rebaudioside A, stevioside, and rubusoside production by yeast

CEY213 containing plasmid pMUS47 after 24 and 99 hours of culture.

FIG. 10A is a graph illustrating the concentrations of RebA, rubusoside and

19-SMG in supernatants. FIG. 10B is a graph of the concentrations of RebA, rubusoside and 19-SMG measured in cell pellets, for experiments where yeast cells were fed with 100 μΜ steviol. In both graphs, the first set of bars represents the untagged control strains; the second set of bars represents the strain containing the UGT74G1, UGT76G1, and UGT91D2e fusion proteins in which the N-terminal 158 amino acids of the MDM2 protein are fused to each UGT, and a UGT85C2 fusion protein in which four repeats of the synthetic PMI peptide is fused in-frame to the Nterminus of 85C2. The y-axis is concentration in micromolar units.

Like reference symbols in the various drawings indicate like elements.

DETAILED DESCRIPTION

Two glycosides, stevioside and rebaudioside A, are the primary compounds in commercially-produced stevia extracts. Stevioside is reported to have a more bitter and less sweet taste than rebaudioside A and, therefore, a higher proportion of rebaudioside A in an extract preparation is preferred. However, the composition of stevia extract can vary from lot to lot depending on the soil and climate in which the plants are grown. Depending upon the sourced plant, the climate conditions, and the extraction process, the amount of rebaudioside A in commercial preparations is reported to vary from 20 to 97% of the total steviol glycoside content, typically >5015

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80% and sometimes as high as >95-97% of the total steviol glycosides. Moreover, other steviol glycosides are present in varying amounts in stevia extracts, which further complicates the ability to produce a sweetener with a consistent taste profde by extraction and purification from Stevia plants. For example, Rebaudioside B is typically present at less than 1-2%, whereas Rebaudioside C can be present at levels as high as 7-15%. Rebaudioside D is typically present in levels of 2% or less, and Rebaudioside F is typically present in compositions at 3.5% or less of the total steviol glycosides. Even trace amounts of the minor steviol glycosides are reported to affect the flavor profile of a Stevia extract. Additionally, it is thought that some of the o contaminants from the Stevia plant, even at very low concentrations, may also provide off-flavors to some of the commercially available plant extracts.

This document is based on the discovery that recombinant hosts such as plant cells, plants, or microorganisms can be developed that express polypeptides useful for the biosynthesis of steviol. Further, such hosts can express Uridine 5'-diphospho (UDP) glycosyl transferases suitable for producing steviol glycosides such as rubusoside and rebaudioside A. Recombinant microorganisms are particularly useful hosts. Expression of these biosynthetic polypeptides in various microbial chassis allows steviol and its glycosides to be produced in a consistent, reproducible manner from energy and carbon sources such as sugars, glycerol, CO2, H₂, and sunlight. The proportion of each steviol glycoside produced by a recombinant host can be tailored by incorporating preselected biosynthetic enzymes into the hosts and expressing them at appropriate levels, to produce a sweetener composition with a consistent taste profile. Furthermore, the concentrations of steviol glycosides produced by recombinant hosts are expected to be higher than the levels of steviol glycosides produced in the Stevia plant, which improves the efficiency of the downstream purification. Such sweetener compositions contain little or no plant based contaminants, relative to the amount of contaminants present in Stevia extracts.

At least one of the genes is a recombinant gene, the particular recombinant gene(s) depending on the species or strain selected for use. Additional genes or biosynthetic modules can be included in order to increase steviol and glycoside yield, improve efficiency with which energy and carbon sources are converted to steviol and its glycosides, and/or to enhance productivity from the cell culture or plant. Such

2018200459 19 Jan 2018 additional biosynthetic modules include genes involved in the synthesis of the terpenoid precursors, isopentenyl diphosphate and dimethylallyl diphosphate.

Additional biosynthetic modules include terpene synthase and terpene cyclase genes, such as genes encoding geranylgeranyl diphosphate synthase and copalyl diphosphate synthase; these genes may be endogenous genes or recombinant genes.

I. Steviol and Steviol Glycoside Biosynthesis Polypeptides

A. Steviol Biosynthesis Polypeptides

Chemical structures for several of the compounds found in Stevia extracts are 10 shown in Fig. 3, including the diterpene steviol and various steviol glycosides. CAS numbers are shown in Table A below. See also, Steviol Glycosides Chemical and Technical Assessment 69th JECFA, prepared by Harriet Wallin, Food Agric. Org. (2007).

Table A.

COMPOUND	CAS#
Steviol	471-80-7
Rebaudioside A	58543-16-1
Steviolbioside	41093-60-1
Stevioside	57817-89-7
Rebaudioside B	58543-17-2
Rebaudioside C	63550-99-2
Rebaudioside D	63279-13-0
Rebaudioside E	63279-14-1
Rebaudioside F	438045-89-7
Rubusoside	63849-39-4
Dulcoside A	64432-06-0

It has been discovered that expression of certain genes in a host such as a microorganism confers the ability to synthesize steviol upon that host. As discussed in more detail below, one or more of such genes may be present naturally in a host. Typically, however, one or more of such genes are recombinant genes that have been transformed into a host that does not naturally possess them.

The biochemical pathway to produce steviol involves formation of geranylgeranyl diphosphate, cyclization to (-) copalyl diphosphate, followed by oxidation and hydroxylation to form steviol. See FIG. 1. Thus, conversion of geranylgeranyl diphosphate to steviol in a recombinant microorganism involves the

2018200459 19 Jan 2018 expression of a gene encoding a kaurene synthase (KS), a gene encoding a kaurene oxidase (KO), and a gene encoding a steviol synthetase (KAH). Steviol synthetase also is known as kaurenoic acid 13-hydroxylase.

Suitable KS polypeptides are known. For example, suitable KS enzymes include those made by Stevia rebaudiana, Zea mays and Populus trichocarpa. See, SEQ ID NOs: 132-135. Nucleotide sequences encoding these polypeptides are described in more detail below. See, for example, Table 3 and SEQ ID NOs: 40-47.

Suitable KO polypeptides are known. For example, suitable KO enzymes include those made by Stevia rebaudiana, Arabidopsis thaliana, Gibberella fujikoroi and Trametes versicolor. See, SEQ ID NOs: 138-141. Nucleotide sequences encoding these polypeptides are described in more detail below. See, for example, Table 5 and SEQ ID NOs: 52-59.

Suitable KAH polypeptides are known. For example, suitable KAH enzymes include those made by Stevia rebaudiana, Arabidopsis thaliana, Vitis vinifera and

Medicago trunculata. See, e.g., SEQ ID NOs: 142-146; U.S. Patent Publication No. 2008-0271205; U.S. Patent Publication No. 2008-0064063 and Genbank Accession No. gi 189098312. The steviol synthetase from Arabidopsis thaliana is classified as a CYP714A2. Nucleotide sequences encoding these polypeptides are described in more detail below. See, for example, Table 6 and SEQ ID NOs: 60-69.

In some embodiments, a recombinant microorganism contains a recombinant gene encoding a KO and/or a KAH polypeptide. Such microorganisms also typically contain a recombinant gene encoding a cytochrome P450 reductase (CPR) polypeptide, since certain combinations of KO and/or KAH polypeptides require expression of an exogenous CPR polypeptide. In particular, the activity of a KO and/or a KAH polypeptide of plant origin can be significantly increased by the inclusion of a recombinant gene encoding an exogenous CPR polypeptide. Suitable CPR polypeptides are known. For example, suitable CPR enzymes include those made by Stevia rebaudiana, Arabidopsis thaliana, and Giberella fujikuroi. See, e.g., SEQ ID NOs: 147-149. Nucleotide sequences encoding these polypeptides are described in more detail below. See, for example, Table 7 and SEQ ID NOs: 70-75.

Expression in a recombinant microorganism of these genes results in the conversion of geranylgeranyl diphosphate to steviol.

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B. Steviol Glycoside Biosynthesis Polypeptides

In some embodiments, a recombinant host described herein can convert steviol to a steviol glycoside. Such a host (e.g., microorganism) contains genes encoding one or more UDP Glycosyl Transferases, also known as UGTs. UGTs transfer a monosaccharide unit from an activated nucleotide sugar to an acceptor moiety, in this case, an -OH or -COOH moiety on steviol or steviol derivative. UGTs have been classified into families and subfamilies based on sequence homology. Li et al. J, Biol, Chem, 276:4338-4343 (2001).

B, 1 Rubusoside Biosynthesis Polypeptides

The biosynthesis of rubusoside involves glycosylation of the 13-OH and the

19-COOH of steviol. See FIG. 2A. It has been discovered that conversion of steviol to rubusoside in a recombinant host such as a microorganism can be accomplished by the expression of gene(s) encoding UGTs 85C2 and 74G1, which transfer a glucose unit to the 13-OH or the 19-COOH, respectively, of steviol.

Thus, a suitable UGT85C2 functions as a uridine 5’-diphospho glucosyl: steviol 13-OH transferase, and a uridine 5’-diphospho glucosyl: steviol-19-(9glucoside 13-OH transferase. Functional UGT85C2 polypeptides also may catalyze glucosyl transferase reactions that utilize steviol glycoside substrates other than steviol and steviol-19-O-glucoside.

A suitable UGT74G1 polypeptide functions as a uridine 5’-diphospho glucosyl: steviol 19-COOH transferase and a uridine 5’-diphospho glucosyl: steviol13-O-glucoside 19-COOH transferase. Functional UGT74G1 polypeptides also may catalyze glycosyl transferase reactions that utilize steviol glycoside substrates other than steviol and steviol-13-O-glucoside, or that transfer sugar moieties from donors other than uridine diphosphate glucose.

A recombinant microorganism expressing a functional UGT74G1 and a functional UGT85C2 can make rubusoside and both steviol monosides (i.e., Steviol

13-O-monoglucoside and Steviol 19-O-monoglucoside) when fed steviol in the medium. One or more of such genes may be present naturally in the host. Typically,

2018200459 19 Jan 2018 however, such genes are recombinant genes that have been transformed into a host (e.g., microorganism) that does not naturally possess them.

As used herein, the term recombinant host is intended to refer to a host, the genome of which has been augmented by at least one incorporated DNA sequence.

Such DNA sequences include but are not limited to genes that are not naturally present, DNA sequences that are not normally transcribed into RNA or translated into a protein (“expressed”), and other genes or DNA sequences which one desires to introduce into the non-recombinant host. It will be appreciated that typically the genome of a recombinant host described herein is augmented through the stable introduction of one or more recombinant genes. Generally, the introduced DNA is not originally resident in the host that is the recipient of the DNA, but it is within the scope of the invention to isolate a DNA segment from a given host, and to subsequently introduce one or more additional copies of that DNA into the same host, e.g., to enhance production of the product of a gene or alter the expression pattern of a gene. In some instances, the introduced DNA will modify or even replace an endogenous gene or DNA sequence by, e.g., homologous recombination or sitedirected mutagenesis. Suitable recombinant hosts include microorganisms, plant cells, and plants.

The term “recombinant gene” refers to a gene or DNA sequence that is introduced into a recipient host, regardless of whether the same or a similar gene or DNA sequence may already be present in such a host. “Introduced,” or “augmented” in this context, is known in the art to mean introduced or augmented by the hand of man. Thus, a recombinant gene may be a DNA sequence from another species, or may be a DNA sequence that originated from or is present in the same species, but has been incorporated into a host by genetic engineering methods to form a recombinant host. It will be appreciated that a recombinant gene that is introduced into a host can be identical to a DNA sequence that is normally present in the host being transformed, and is introduced to provide one or more additional copies of the DNA to thereby permit overexpression or modified expression of the gene product of that DNA.

Suitable UGT74G1 and UGT85C2 polypeptides include those made by Stevia rebaudiana. Genes encoding functional UGT74G1 and UGT85C2 polypeptides from

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Stevia are reported in Richman, et al. Plant J, 41: 56-67 (2005). Amino acid sequences of S. rebaudiana UGT74G1 and UGT85C2 polypeptides are set forth in

SEQ ID NOs: 1 and 3, respectively. Nucleotide sequences encoding UGT74G1 and

UGT85C2 that have been optimized for expression in yeast are set forth in SEQ ID

NOs: 2 and 4, respectively. See also the UGT85C2 and UGT74G1 variants described in Examples 17 and 18, respectively.

In some embodiments, the recombinant host is a microorganism. The recombinant microorganism can be grown on media containing steviol in order to produce rubusoside. In other embodiments, however, the recombinant microorganism expresses one or more recombinant genes involved in steviol biosynthesis, e.g., a CDPS gene, a KS gene, a KO gene and/or a KAH gene. Thus, a microorganism containing a CDPS gene, a KS gene, a KO gene and a KAH gene in addition to a UGT74G1 and aUGT85C2 gene is capable of producing both steviol monosides and rubusoside without the necessity for including steviol in the culture media.

In some embodiments, the recombinant microorganism further expresses a recombinant gene encoding a geranylgeranyl diphosphate synthase (GGPPS).

Suitable GGPPS polypeptides are known. For example, suitable GGPPS enzymes include those made by Stevia rebaudiana, Gibberella fujikuroi, Mus musculus, Thalassiosira pseudonana, Streptomyces clavuligerus, Sulfulobus acidocaldarius,

Synechococcus sp. and Arabidopsis thaliana. See, SEQ ID NOs: 121-128.

Nucleotide sequences encoding these polypeptides are described in more detail below. See Table 1 and SEQ ID NOs: 18-33. In some embodiments, the recombinant microorganism further expresses recombinant genes involved in diterpene biosynthesis or production of terpenoid precursors, e.g., genes in the methylerythritol

4-phosphate (MEP) pathway or genes in the mevalonate (MEV) pathway discussed below.

B, 2 Rebaudioside A Biosynthesis Polypeptides

The biosynthesis of rebaudioside A involves glucosylation of the aglycone steviol. Specifically, rebaudioside A can be formed by glucosylation of the 13-OH of steviol which forms the 13-O-steviolmonoside, glucosylation of the C-2’ of the 13-0glucose of steviolmonoside which forms steviol-1,2-bioside, glucosylation of the C21

2018200459 19 Jan 2018 carboxyl of steviol-1,2-bioside which forms stevioside, and glucosylation of the C3’ of the C-13-O-glucose of stevioside. The order in which each glucosylation reaction occurs can vary. See FIG. 2A.

It has been discovered that conversion of steviol to rebaudioside A in a recombinant host can be accomplished by the expression of gene(s) encoding the following functional UGTs: 74G1, 85C2, 76G1 and 91D2. Thus, a recombinant microorganism expressing these four UGTs can make rebaudioside A when fed steviol in the medium. Typically, one or more of these genes are recombinant genes that have been transformed into a microorganism that does not naturally possess them. It has also been discovered that UGTs designated herein as SM12UGT can be substituted for UGT91D2.

Suitable UGT74G1 and UGT85C2 polypeptides include those discussed above. A suitable UGT76G1 adds a glucose moiety to the C-3’of the C-13-O-glucose of the acceptor molecule, a steviol 1,2 glycoside. Thus, UGT76G1 functions, for example, as a uridine 5’-diphospho glucosyl: steviol 13-0-1,2 glucoside C-3’ glucosyl transferase and a uridine 5’-diphospho glucosyl: steviol-19-O-glucose, 13-0-1,2 bioside C-3’ glucosyl transferase. Functional UGT76G1 polypeptides may also catalyze glucosyl transferase reactions that utilize steviol glycoside substrates that contain sugars other than glucose, e.g., steviol rhamnosides and steviol xylosides.

See, FIGS 2A, 2B, 2C and 2D. Suitable UGT76G1 polypeptides include those made by S. rebaudiana and reported in Richman, et al. Plant J, 41: 56-67 (2005). The amino acid sequence of a S. rebaudiana UGT76G1 polypeptide is set forth in SEQ ID NO:7. The nucleotide sequence encoding the UGT76G1 polypeptide of SEQ ID NO:7 has been optimized for expression in yeast and is set forth in SEQ ID NO:8.

See also the UGT76G1 variants set forth in Example 18.

A suitable UGT91D2 polypeptide functions as a uridine 5’-diphospho glucosyl: steviol-13-O-glucoside transferase (also referred to as a steviol-13monoglucoside 1,2-glucosylase), transferring a glucose moiety to the C-2’ of the 13O-glucose of the acceptor molecule, steviol-13-O-glucoside. Typically, a suitable

UGT91D2 polypeptide also functions as a uridine 5’-diphospho glucosyl: rubusoside transferase transferring a glucose moiety to the C-2’ of the 13-O-glucose of the acceptor molecule, rubusoside.

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Functional UGT91D2 polypeptides may also catalyze reactions that utilize steviol glycoside substrates other than steviol-13-O-glucoside and rubusoside, e.g., functional UGT91D2 polypeptides may utilize stevioside as a substrate, transferring a glucose moiety to the C-2’ of the 19-O-glucose residue to produce Rebaudioside E.

Functional UGT91D2 polypeptides may also utilize Rebaudioside A as a substrate, transferring a glucose moiety to the C-2’ of the 19-O-glucose residue to produce Rebaudioside D. However, a functional UGT91D2 polypeptide typically does not transfer a glucose moiety to steviol compounds having a 1,3-bound glucose at the C13 position, i.e., transfer of a glucose moiety to steviol 1,3-bioside and 1,3-stevioside does not occur.

Functional UGT91D2 polypeptides can transfer sugar moieties from donors other than uridine diphosphate glucose. For example, a functional UGT91D2 polypeptide can act as a uridine 5’-diphospho D-xylosyl: steviol-13-O-glucoside transferase, transferring a xylose moiety to the C-2’ of the 13-O-glucose of the acceptor molecule, steviol-13-O-glucoside. As another example, a functional

UGT91D2 polypeptide can act as a uridine 5’-diphospho L-rhamnosyl: steviol-13-Oglucoside transferase, transferring a rhamnose moiety to the C-2’ of the 13-O-glucose of the acceptor molecule, steviol-13-O-glucoside

Suitable functional UGT91D2 polypeptides include those disclosed herein,

e.g., the polypeptides designated UGT91D2e and UGT91D2m. The amino acid sequence of an exemplary UGT91D2e polypeptide from Stevia rebaudiana is set forth in SEQ ID NO: 5. SEQ ID NO:6 is a nucleotide sequence encoding the polypeptide of SEQ ID NO:5 that has been codon optimized for expression in yeast. The S. rebaudiana nucleotide sequence encoding the polypeptide of SEQ ID NO:5 is set forth in SEQ ID NO:9. The amino acid sequences of exemplary UGT91D2m polypeptides from S. rebaudiana are set forth in SEQ ID NOs: 10 and 12, and are encoded by the nucleic acid sequences set forth in SEQ ID NOs: 11 and 13, respectively. See also the UGT91D2 variants of Example 16, e.g., a variant containing a substitution at amino acid residues 206, 207, and 343.

As indicated above, UGTs designated herein as SM12UGT can be substituted for UGT91D2. Suitable functional SM12UGT polypeptides include those made by Ipomoea purpurea (Japanese morning glory) and described in Morita et al. Plant J, 42,

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353-363 (2005). The amino acid sequence encoding the I. purpurea IP3GGT polypeptide is set forth in SEQ ID NO:76. SEQ ID NO:77 is a nucleotide sequence encoding the polypeptide of SEQ ID NO:76 that has been codon optimized for expression in yeast. Another suitable SM12UGT polypeptide is a Bp94Bl polypeptide having an R25S mutation. See Osmani et al. Plant Phys. 148: 1295-1308 (2008) and Sawada et al. J, Biol, Chem, 280:899-906 (2005). The amino acid sequence encoding the Beilisperennis (red daisy) UGT94B1 polypeptide is set forth in SEQ ID NO:78. SEQ ID NO:79 is the nucleotide sequence encoding the polypeptide of SEQ ID NO:78 that has been codon optimized for expression in yeast.

o In some embodiments, the recombinant microorganism is grown on media containing steviol-13-O-glucoside or steviol-19-O-glucoside in order to produce rebaudioside A. In such embodiments, the microorganism contains and expresses genes encoding a functional UGT91D2, a functional UGT74G1 and a functional UGT76G1, and is capable of producing rebaudioside A when it is fed steviol, one or both of the steviolmonosides, or rubusoside in the culture media.

In other embodiments, the recombinant microorganism is grown on media containing rubusoside in order to produce rebaudioside A. In such embodiments, the microorganism contains and expresses genes encoding a functional UGT91D2 and a functional UGT76G1, and is capable of producing rebaudioside A when it is fed rubusoside in the culture media.

In other embodiments the recombinant microorganism expresses one or more genes involved in steviol biosynthesis, e.g., a CDPS gene, a KS gene, a KO gene and/or a KAH gene. Thus, for example, a microorganism containing a CDPS gene, a KS gene, a KO gene and a KAH gene, in addition to a UGT74G1, a UGT85C2, a

UGT91D2 gene and a UGT76G1 gene, is capable of producing rebaudioside A without the necessity for including steviol in the culture media.

In some embodiments, the recombinant microorganism further contains and expresses a recombinant GGPPS gene in order to provide increased levels of the diterpene precursor geranylgeranyl diphosphate, for increased flux through the rebaudioside A biosynthetic pathway. In some embodiments, the recombinant microorganism further contains and expresses recombinant genes involved in

2018200459 19 Jan 2018 diterpene biosynthesis or production of terpenoid precursors, e.g., genes in the MEP or MEV pathway discussed below.

B, 3 Dulcoside A and Rebaudioside C Biosynthesis Polypeptides

The biosynthesis of rebaudioside C and/or dulcoside A involves glucosylation and rhamnosylation of the aglycone steviol. Specifically, dulcoside A can be formed by glucosylation of the 13-OH of steviol which forms steviol-13-O-glucoside, rhamnosylation of the C-2’ of the 13-O-glucose of steviol-13-O-glucoside which forms the 1,2 rhamnobioside, and glucosylation of the C-19 carboxyl of the 1,2 o rhamnobioside. Rebaudioside C can be formed by glucosylation of the C-3 ’ of the C13-O-glucose of dulcoside A. The order in which each glycosylation reaction occurs can vary. See FIG. 2B.

It has been discovered that conversion of steviol to dulcoside A in a recombinant host can be accomplished by the expression of gene(s) encoding the following functional UGTs: 85C2, 91D2, and 74G1. Thus, a recombinant microorganism expressing these three UGTs and a rhamnose synthetase can make dulcoside A when fed steviol in the medium. Alternatively, a recombinant microorganism expressing two UGTs, 91D2 and 74G1, and rhamnose synthetase can make dulcoside A when fed the monoside, steviol-13-O-glucoside or steviol-19-O20 glucoside, in the medium. Similarly, conversion of steviol to rebaudioside C in a recombinant microorganism can be accomplished by the expression of gene(s) encoding UGTs 85C2, 91D2, 74G1, and 76G1 and rhamnose synthetase when fed steviol, by the expression of genes encoding UGTs 91D2, 74G1 and 76G1, and rhamnose synthetase when fed steviol-13-(9-glucosidc, by the expression of genes encoding UGTs 85C2, 91D2 and 76G1, and rhamnose synthetase when fed steviol19-O-glucoside, or by the expression of genes encoding UGTs 91D2 and 76G1 and rhamonse synthetase when fed rubusoside. Typically, one or more of these genes are recombinant genes that have been transformed into a microorganism that does not naturally possess them.

Suitable UGT91D2, UGT74G1, UGT76G1 and UGT85C2 polypeptides include the functional UGT polypeptides discussed herein. Rhamnose synthetase provides increased amounts of the UDP-rhamnose donor for rhamnosylation of the

2018200459 19 Jan 2018 steviol compound acceptor. Suitable rhamnose synthetases include those made by

Arabidopsis thaliana, such as the product of the A. thaliana RHM2 gene.

In some embodiments, a UGT79B3 polypeptide is substituted for a UGT91D2 polypeptide. Suitable UGT79B3 polypeptides include those made by Arabidopsis thaliana, which are capable of rhamnosylation of steviol 13-O-monoside in vitro. A. thaliana UGT79B3 can rhamnosylate glucosylated compounds to form 1,2rhamnosides. The amino acid sequence of an Arabidopsis thaliana UGT79B3 is set forth in SEQ ID NO: 150. The nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 150 is set forth in SEQ ID NO: 151.

In some embodiments rebaudioside C can be produced using in vitro methods while supplying the appropriate UDP-sugar or a cell-free system for regeneration of UDP-sugars. See, for example, “An integrated cell-free metabolic platform for protein production and synthetic biology” by Jewett MC, Calhoun KA, Voloshin A, Wuu JJ and Swartz JR in Molecular Systems Biology, 4, article 220 (2008).

Reactions may be carried out together, or stepwise. For instance, rebaudioside C may be produced from rubusoside with the addition of stoichiometric amounts of UDPrhamnose and UGT91d2e, followed by addition of UGT76G1 and an excess or stoichiometric supply of UDP-glucose. In some embodiments phosphatases are used to remove secondary products and improve the reaction yields.

In other embodiments, the recombinant host expresses one or more genes involved in steviol biosynthesis, e.g., a CDPS gene, a KS gene, a KO gene and/or a KAH gene. Thus, for example, a microorganism containing a CDPS gene, a KS gene, a KO gene and a KAH gene, in addition to a UGT85C2, a UGT74G1, a UGT91D2 gene and a UGT76G1 gene, is capable of producing rebaudioside C without the necessity for including steviol in the culture media. In addition, the recombinant host typically expresses an endogenous or a recombinant gene encoding a rhamnose synthetase. Such a gene is useful in order to provide increased amounts of the UDPrhamnose donor for rhamnosylation of the steviol compound acceptor. Suitable rhamnose synthetases include those made by Arabidopsis thaliana, such as the product of the A. thaliana RHM2 gene.

One with skill in the art will recognize that by modulating relative expression levels of different UGT genes as well as modulating the availability of UDP26

2018200459 19 Jan 2018 rhamnose, a recombinant host can be tailored to specifically produce steviol and steviol glycoside products in a desired proportion. Transcriptional regulation of steviol biosynthesis genes, and steviol glycoside biosynthesis genes can be achieved by a combination of transcriptional activation and repression using techniques known to those in the art. For in vitro reactions, one with skill in the art will recognize that addition of different levels of UGT enzymes in combination or under conditions which impact the relative activities of the different UGTS in combination will direct synthesis towards a desired proportion of each steviol glycoside.

In some embodiments, the recombinant host further contains and expresses a recombinant GGPPS gene in order to provide increased levels of the diterpene precursor geranylgeranyl diphosphate, for increased flux through the rebaudioside A biosynthetic pathway. In some embodiments, the recombinant host further contains a genetic construct to silence or reduce the expression of non-steviol pathways consuming geranylgeranyl diphosphate, ent-Kaurenoic acid or farnesyl pyrophosphate, thereby providing increased flux through the steviol and steviol glycosides biosynthetic pathways. For example, flux to sterol production pathways such as ergosterol may be reduced by downregulation of the ERG9 gene. In cells that produce gibberellins, gibberellin synthesis may be downregulated to increase flux of ent-kaurenoic acid to steviol. In carotenoid-producing organisms, flux to steviol may be increased by downregulation of one or more carotenoid biosynthetic genes.

In some embodiments, the recombinant host further contains and expresses recombinant genes involved in diterpene biosynthesis or production of terpenoid precursors, e.g., genes in the MEP or MEV pathway discussed below.

In some embodiments, a recombinant host such as a microorganism produces steviol glycoside compositions that have greater than at least 15% rebaudioside C of the total steviol glycosides, e.g., at least 20% rebaudioside C, 30-40% rebaudioside C, 40-50% rebaudioside C, 50-60% rebaudioside C, 60-70% rebaudioside C, 70-80% rebaudioside C, 80-90% rebaudioside C. In some embodiments, a recombinant host such as a microorganism produces steviol glycoside compositions that have at least

90% rebaudioside C, e.g., 90-99% rebaudioside C. Other steviol glycosides present may include those depicted in Figures 2 A and B such as steviol monosides, steviol glucobiosides, steviol rhamnobiosides, rebaudioside A, and Dulcoside A. In some

2018200459 19 Jan 2018 embodiments, the rebaudioside C-enriched composition produced by the host can be further purified and the rebaudioside C or Dulcoside A so purified may then be mixed with other steviol glycosides, flavors, or sweeteners to obtain a desired flavor system or sweetening composition. For instance, a rebaudioside C-enriched composition produced by a recombinant microorganism can be combined with a rebaudioside A, F, or D-enriched composition produced by a different recombinant microorganism, with rebaudioside A, F, or D purified from a Stevia extract, or with rebaudioside A, F, or D produced in vitro.

B, 4 Rebaudioside E and Rebaudioside D Biosynthesis Polypeptides

The biosynthesis of rebaudioside E and/or rebaudioside D involves glucosylation of the aglycone steviol. Specifically, rebaudioside E can be formed by glucosylation of the 13-OH of steviol which forms steviol-13-O-glucoside, glucosylation of the C-2’ of the 13-O-glucose of steviol-13-O-glucoside which forms the steviol-1,2-bioside, glucosylation of the C-19 carboxyl of the 1,2-bioside to form

1.2- stevioside, and glucosylation of the C-2’ of the 19-O-glucose of the 1,2-stevioside to form rebaudioside E. Rebaudioside D can be formed by glucosylation of the C-3 ’ of the C-13-O-glucose of rebaudioside E. The order in which each glycosylation reaction occurs can vary. For example, the glucosylation of the C-2’ of the 19-020 glucose may be the last step in the pathway, wherein Rebaudioside A is an intermediate in the pathway. See FIG. 2C.

It has been discovered that conversion of steviol to rebaudioside D in a recombinant host can be accomplished by the expression of gene(s) encoding the following functional UGTs: 85C2, 91D2, 74G1 and 76G1. Thus, a recombinant microorganism expressing these four UGTs can make rebaudioside D when fed steviol in the medium. Alternatively, a recombinant microorganism expressing two functional UGTs, 91D2 and 76G1, can make rebaudioside D when fed rubusoside or

1.2- stevioside in the medium. As another alternative, a recombinant microorganism expressing three functional UGTs, 74G1, 91D2 and 76G1, can make rebaudioside D when fed the monoside, steviol-13-O-glucoside, in the medium. Similarly, conversion of steviol-19-O-glucoside to rebaudioside D in a recombinant microorganism can be accomplished by the expression of genes encoding UGTs 85C2, 91D2 and 76G1

2018200459 19 Jan 2018 when fed steviol-19-O-glucoside. Typically, one or more of these genes are recombinant genes that have been transformed into a host that does not naturally possess them.

Suitable UGT91D2, UGT74G1, UGT76G1 and UGT85C2 polypeptides include the functional UGT polypeptides discussed herein. In some embodiments, a UGT79B3 polypeptide is substituted for aUGT91, as discussed above.

In some embodiments, rebaudioside D or rebaudioside E can be produced using in vitro methods while supplying the appropriate UDP-sugar or a cell-free system for regeneration of UDP-sugars. See, for example, Jewett MC, et al.

Molecular Systems Biology, Vol. 4, article 220 (2008). Conversions requiring multiple reactions may be carried out together, or stepwise. Rebaudioside D may be produced from Rebaudioside A that is commercially available enriched extract or produced via biosynthesis, with the addition of stoichiometric or excess amounts of UDP-glucose and UGT91D2e. In some embodiments phosphatases are used to remove secondary products and improve the reaction yields.

One with skill in the art will recognize that by modulating relative expression levels of different UGT genes, a recombinant host can be tailored to specifically produce steviol and steviol glycoside products in a desired proportion.

Transcriptional regulation of steviol biosynthesis genes and steviol glycoside biosynthesis genes can be achieved by a combination of transcriptional activation and repression using techniques known to those in the art. For in vitro reactions, one with skill in the art will recognize that addition of different levels of UGT enzymes in combination or under conditions which impact the relative activities of the different UGTS in combination will direct synthesis towards a desired proportion of each steviol glycoside. One with skill in the art will recognize that a higher proportion of rebaudioside D or E or more efficient conversion to rebaudioside D or E can be obtained with a diglycosylation enzyme that has a higher activity for the 19-0glucoside reaction as compared to the 13-O-glucoside reaction (substrates rebaudioside A and stevioside).

In other embodiments, the recombinant host expresses one or more genes involved in steviol biosynthesis, e.g., a CDPS gene, a KS gene, a KO gene and/or a KAH gene. Thus, for example, a microorganism containing a CDPS gene, a KS gene,

2018200459 19 Jan 2018 a KO gene and a KAH gene, in addition to a UGT85C2, a UGT74G1, a UGT91D2 gene and a UGT76G1 gene, is capable of producing rebaudiosides E and D without the necessity for including steviol in the culture media.

In some embodiments, the recombinant host further contains and expresses a recombinant GGPPS gene in order to provide increased levels of the diterpene precursor geranylgeranyl diphosphate, for increased flux through the steviol biosynthetic pathway. In some embodiments, the recombinant host further contains a genetic construct to silence the expression of non-steviol pathways consuming geranylgeranyl diphosphate, ent-Kaurenoic acid or farnesyl pyrophosphate, thereby providing increased flux through the steviol and steviol glycosides biosynthetic pathways. For example, flux to sterol production pathways such as ergosterol may be reduced by downregulation of the ERG9 gene. In cells that produce gibberellins, gibberellin synthesis may be downregulated to increase flux of ent-kaurenoic acid to steviol. In carotenoid-producing organisms, flux to steviol may be increased by downregulation of one or more carotenoid biosynthetic genes. In some embodiments, the recombinant host further contains and expresses recombinant genes involved in diterpene biosynthesis or production of terpenoid precursors, e.g., genes in the MEP or MEV pathways discussed below.

In some embodiments, a recombinant host such as a microorganism produces rebaudioside D-enriched steviol glycoside compositions that have greater than at least 3% rebaudioside D by weight total steviol glycosides, e.g., at least 4% rebaudioside D at least 5% rebaudioside D, 10-20% rebaudioside D, 20-30% rebaudioside D, 30-40% rebaudioside D, 40-50% rebaudioside D, 50-60% rebaudioside D, 60-70% rebaudioside D, 70-80% rebaudioside D . In some embodiments, a recombinant host such as a microorganism produces steviol glycoside compositions that have at least 90% rebaudioside D, e.g., 90-99% rebaudioside D. Other steviol glycosides present may include those depicted in Figure 2 C such as steviol monosides, steviol glucobiosides, rebaudioside A, rebaudioside E, and stevioside. In some embodiments, the rebaudioside D-enriched composition produced by the host (e.g., microorganism) can be further purified and the rebaudioside D or rebaudioside E so purified can then be mixed with other steviol glycosides, flavors, or sweeteners to obtain a desired flavor system or sweetening composition. For instance, a rebaudioside D-enriched

2018200459 19 Jan 2018 composition produced by a recombinant host can be combined with a rebaudioside A,

C, or F-enriched composition produced by a different recombinant host, with rebaudioside A, F, or C purified from a Stevia extract, or with rebaudioside A, F, or C produced in vitro.

B, 5 Rebaudioside F Biosynthesis Polypeptides

The biosynthesis of rebaudioside F involves glucosylation and xylosylation of the aglycone steviol. Specifically, rebaudioside F can be formed by glucosylation of the 13-OH of steviol which forms steviol-13-O-glucoside, xylosylation ofthe C-2’ of the 13-O-glucose of steviol-13-O-glucoside which forms steviol-1,2-xylobioside, glucosylation ofthe C-19 carboxyl ofthe 1,2-xylobioside to form 1,2-stevioxyloside, and glucosylation ofthe C-3’ ofthe C-13-O-glucose of 1,2-stevioxyloside to form rebaudioside F. The order in which each glycosylation reaction occurs can vary. See FIG. 2D.

It has been discovered that conversion of steviol to rebaudioside F in a recombinant host can be accomplished by the expression of genes encoding the following functional UGTs: 85C2, 91D2, 74G1 and 76G1, along with endogenous or recombinantly expressed UDP-glucose dehydrogenase and UDP-glucuronic acid decarboxylase. Thus, a recombinant microorganism expressing these four UGTs along with endogenous or recombinant UDP-glucose dehydrogenase and UDPglucuronic acid decarboxylase can make rebaudioside F when fed steviol in the medium. Alternatively, a recombinant microorganism expressing two functional UGTs, 91D2 and 76G1, can make rebaudioside F when fed rubusoside in the medium. As another alternative, a recombinant microorganism expressing a functional UGT

76G1 can make rebaudioside F when fed 1,2 steviorhamnoside. As another alternative, a recombinant microorganism expressing three functional UGTs, 74G1, 91D2 and 76G1, can make rebaudioside F when fed the monoside, steviol-13-Oglucoside, in the medium. Similarly, conversion of steviol-19-O-glucoside to rebaudioside F in a recombinant microorganism can be accomplished by the expression of genes encoding UGTs 85C2, 91D2 and 76G1 when fed steviol-19-0glucoside. Typically, one or more of these genes are recombinant genes that have been transformed into a host that does not naturally possess them.

2018200459 19 Jan 2018

Suitable UGT91D2, UGT74G1, UGT76G1 and UGT85C2 polypeptides include the functional UGT polypeptides discussed herein. In some embodiments, a

UGT79B3 polypeptide is substituted for aUGT91, as discussed above. UDP-glucose dehydrogenase and UDP-glucuronic acid decarboxylase provide increased amounts of the UDP-xylose donor for xylosylation of the steviol compound acceptor. Suitable

UDP-glucose dehydrogenases and UDP-glucuronic acid decarboxylases include those made by Arabidopsis thaliana or Cryptococcus neoformans. For example, suitable UDP-glucose dehydrogenase and UDP-glucuronic acid decarboxylases polypeptides can be encoded by the A. thaliana UGD1 gene and UXS3 gene, respectively. See,

Oka and Jigami, FEBS J. 273:2645-2657 (2006).

In some embodiments rebaudioside F can be produced using in vitro methods while supplying the appropriate UDP-sugar or a cell-free system for regeneration of UDP-sugars. See, for example, Jewett MC, et al. Molecular Systems Biology, Vol. 4, article 220 (2008). Reactions may be carried out together, or stepwise. For instance, rebaudioside F may be produced from rubusoside with the addition of stoichiometric amounts of UDP-xylose and UGT91D2e, followed by addition of UGT76G1 and an excess or stoichiometric supply of UDP-glucose. In some embodiments phosphatases are used to remove secondary products and improve the reaction yields.

In other embodiments, the recombinant host expresses one or more genes involved in steviol biosynthesis, e.g., a CDPS gene, a KS gene, a KO gene and/or a

KAH gene. Thus, for example, a microorganism containing a CDPS gene, a KS gene, a KO gene and a KAH gene, in addition to a UGT85C2, a UGT74G1, a UGT91D2 gene and a UGT76G1 gene, is capable of producing rebaudioside F without the necessity for including steviol in the culture media. In addition, the recombinant host typically expresses an endogenous or a recombinant gene encoding a UDP-glucose dehydrogenase and a UDP-glucuronic acid decarboxylase. Such genes are useful in order to provide increased amounts of the UDP-xylose donor for xylosylation of the steviol compound acceptor. Suitable UDP-glucose dehydrogenases and UDPglucuronic acid decarboxylases include those made by Arabidopsis thaliana or

Cryptococcus neoformans. For example, suitable UDP-glucose dehydrogenase and UDP-glucuronic acid decarboxylases polypeptides can be encoded by the A. thaliana

2018200459 19 Jan 2018

UGDl gene and UXS3 gene, respectively. See, Oka and Jigami, FEBS J. 273:26452657 (2006).

One with skill in the art will recognize that by modulating relative expression levels of different UGT genes as well as modulating the availability of UDP-xylose, a recombinant microorganism can be tailored to specifically produce steviol and steviol glycoside products in a desired proportion. Transcriptional regulation of steviol biosynthesis genes can be achieved by a combination of transcriptional activation and repression using techniques known to those in the art. For in vitro reactions, one with skill in the art will recognize that addition of different levels of UGT enzymes in o combination or under conditions which impact the relative activities of the different UGTS in combination will direct synthesis towards a desired proportion of each steviol glycosides.

In some embodiments, the recombinant host further contains and expresses a recombinant GGPPS gene in order to provide increased levels ofthe diterpene precursor geranylgeranyl diphosphate, for increased flux through the steviol biosynthetic pathway. In some embodiments, the recombinant host further contains a genetic construct to silence the expression of non-steviol pathways consuming geranylgeranyl diphosphate, ent-Kaurenoic acid or farnesyl pyrophosphate, thereby providing increased flux through the steviol and steviol glycosides biosynthetic pathways. For example, flux to sterol production pathways such as ergosterol may be reduced by downregulation of the ERG9 gene. In cells that produce gibberellins, gibberellin synthesis may be downregulated to increase flux of ent-kaurenoic acid to steviol. In carotenoid-producing organisms, flux to steviol may be increased by downregulation of one or more carotenoid biosynthetic genes. In some embodiments, the recombinant host further contains and expresses recombinant genes involved in diterpene biosynthesis, e.g., genes in the MEP pathway discussed below.

In some embodiments, a recombinant host such as a microorganism produces rebaudioside F-enriched steviol glycoside compositions that have greater than at least 4% rebaudioside F by weight total steviol glycosides, e.g., at least 5% rebaudioside F, at least 6% of rebaudioside F, 10-20% rebaudioside F, 20-30% rebaudioside F, 3040% rebaudioside F, 40-50% rebaudioside F, 50-60% rebaudioside F, 60-70% rebaudioside F, 70-80% rebaudioside F. In some embodiments, a recombinant host

2018200459 19 Jan 2018 such as a microorganism produces steviol glycoside compositions that have at least

90% rebaudioside F, e.g., 90-99% rebaudioside F. Other steviol glycosides present may include those depicted in Figure 2 A and D such as steviol monosides, steviol glucobiosides, steviol xylobiosides, rebaudioside A, stevioxyloside, rubusoside and stevioside. In some embodiments, the rebaudioside F-enriched composition produced by the host can be mixed with other steviol glycosides, flavors, or sweeteners to obtain a desired flavor system or sweetening composition. For instance, a rebaudioside F-enriched composition produced by a recombinant microorganism can be combined with a rebaudioside A, C, or D-enriched composition produced by a o different recombinant microorganism, with rebaudioside A, C, or D purified from a Stevia extract, or with rebaudioside A, C, or D produced in vitro.

C. Other Polypeptides

Genes for additional polypeptides whose expression facilitates more efficient or larger scale production of steviol or a steviol glycoside can also be introduced into a recombinant host. For example, a recombinant microorganism, plant, or plant cell can also contain one or more genes encoding a geranylgeranyl diphosphate synthase (GGPPS, also referred to as GGDPS). As another example, the recombinant host can contain one or more genes encoding a rhamnose synthetase, or one or more genes encoding a UDP-glucose dehydrogenase and/or a UDP-glucuronic acid decarboxylase. As another example, a recombinant host can also contain one or more genes encoding a cytochrome P450 reductase (CPR). Expression of a recombinant CPR facilitates the cycling of NADP+ to regenerate NADPH, which is utilized as a cofactor for terpenoid biosynthesis. Other methods can be used to regenerate NADHP levels as well. In circumstances where NADPH becomes limiting; strains can be further modified to include exogenous transhydrogenase genes. See, e.g., Sauer et al., J. Biol, Chem, 279: 6613-6619 (2004). Other methods are known to those with skill in the art to reduce or otherwise modify the ratio of NADH/NADPH such that the desired cofactor level is increased.

As another example, the recombinant host can contain one or more genes encoding one or more enzymes in the MEP pathway or the mevalonate pathway.

Such genes are useful because they can increase the flux of carbon into the diterpene

2018200459 19 Jan 2018 biosynthesis pathway, producing geranylgeranyl diphosphate from isopentenyl diphosphate and dimethylallyl diphosphate generated by the pathway. The geranylgeranyl diphosphate so produced can be directed towards steviol and steviol glycoside biosynthesis due to expression of steviol biosynthesis polypeptides and steviol glycoside biosynthesis polypeptides.

C. 1 MEP Biosynthesis Polypeptides

In some embodiments, a recombinant host contains one or more genes encoding enzymes involved in the methylerythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis. Enzymes in the MEP pathway include deoxyxylulose 5phosphate synthase (DXS), D-l-deoxyxylulose 5-phosphate reductoisomerase (DXR), 4-diphosphocytidyl-2-C-methyl-D-erythritol synthase (CMS), 4-diphosphocytidyl-2C-methyl-D-erythritol kinase (CMK), 4-diphosphocytidyl-2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MCS), 1-hydroxy-2-methyl-2(E)-butenyl 415 diphosphate synthase (HDS) and 1-hydroxy-2-methyl-2(E)-butenyl 4-diphosphate reductase (HDR). One or more DXS genes, DXR genes, CMS genes, CMK genes, MCS genes, HDS genes and/or HDR genes can be incorporated into a recombinant microorganism. See. Rodriguez-Concepcion and Boronat. Plant Phys. 130: 10791089 (2002).

Suitable genes encoding DXS, DXR, CMS, CMK, MCS, HDS and/or HDR polypeptides include those made by E. coli, Arabidopsis thaliana and Synechococcus leopoliensis. Nucleotide sequences encoding DXR polypeptides are described, for example, in U.S. Patent No. 7,335,815.

C. 2 Mevalonate Biosynthesis Polypeptides

In some embodiments, a recombinant host contains one or more genes encoding enzymes involved in the mevalonate pathway for isoprenoid biosynthesis. Genes suitable for transformation into a host encode enzymes in the mevalonate pathway such as a truncated 3-hydroxy-3-methyl-glutaryl (HMG)-CoA reductase (tHMG), and/or a gene encoding a mevalonate kinase (MK), and/or a gene encoding a phosphomevalonate kinase (PMK), and/or a gene encoding a mevalonate pyrophosphate decarboxylase (MPPD). Thus, one or more HMG-CoA reductase

2018200459 19 Jan 2018 genes, MK genes, PMK genes, and/or MPPD genes can be incorporated into a recombinant host such as a microorganism.

Suitable genes encoding mevalonate pathway polypeptides are known. For example, suitable polypeptides include those made by E. coli, Paracoccus denitriftcans, Saccharomyces cerevisiae, Arabidopsis thaliana, Kitasatospora griseola, Homo sapiens, Drosophila melanogaster, Gallus gallus, Streptomyces sp. KO-3988, Nicotiana attenuata, Kitasatospora griseola, Hevea brasiliensis, Enterococcus faecium and Haematococcuspluvialis. See, e.g., U.S. Patent Nos. 7,183,089, 5,460,949, and 5,306,862.

D. Functional Homologs

Functional homologs of the polypeptides described above are also suitable for use in producing steviol or steviol glycosides in a recombinant host. A functional homolog is a polypeptide that has sequence similarity to a reference polypeptide, and that carries out one or more of the biochemical or physiological function(s) of the reference polypeptide. A functional homolog and the reference polypeptide may be natural occurring polypeptides, and the sequence similarity may be due to convergent or divergent evolutionary events. As such, functional homologs are sometimes designated in the literature as homologs, or orthologs, or paralogs. Variants of a naturally occurring functional homolog, such as polypeptides encoded by mutants of a wild type coding sequence, may themselves be functional homologs. Functional homologs can also be created via site-directed mutagenesis of the coding sequence for a polypeptide, or by combining domains from the coding sequences for different naturally-occurring polypeptides (“domain swapping”). Techniques for modifying genes encoding functional UGT polypeptides described herein are known and include, inter alia, directed evolution techniques, site-directed mutagenesis techniques and random mutagenesis techniques, and can be useful to increase specific activity of a polypeptide, alter substrate specificity, alter expression levels, alter subcellular location, or modify polypeptide:polypeptide interactions in a desired manner. Such modified polypeptides are considered functional homologs. The term “functional homolog” is sometimes applied to the nucleic acid that encodes a functionally homologous polypeptide.

2018200459 19 Jan 2018

Functional homologs can be identified by analysis of nucleotide and polypeptide sequence alignments. For example, performing a query on a database of nucleotide or polypeptide sequences can identify homologs of steviol or steviol glycoside biosynthesis polypeptides. Sequence analysis can involve BLAST,

Reciprocal BLAST, or PSI-BLAST analysis of nonredundant databases using a GGPPS, a CDPS, a KS, a KO or a KAH amino acid sequence as the reference sequence. Amino acid sequence is, in some instances, deduced from the nucleotide sequence. Those polypeptides in the database that have greater than 40% sequence identity are candidates for further evaluation for suitability as a steviol or steviol glycoside biosynthesis polypeptide. Amino acid sequence similarity allows for conservative amino acid substitutions, such as substitution of one hydrophobic residue for another or substitution of one polar residue for another. If desired, manual inspection of such candidates can be carried out in order to narrow the number of candidates to be further evaluated. Manual inspection can be performed by selecting those candidates that appear to have domains present in steviol biosynthesis polypeptides, e.g., conserved functional domains.

Conserved regions can be identified by locating a region within the primary amino acid sequence of a steviol or a steviol glycoside biosynthesis polypeptide that is a repeated sequence, forms some secondary structure (e.g., helices and beta sheets), establishes positively or negatively charged domains, or represents a protein motif or domain. See, e.g., the Pfam web site describing consensus sequences for a variety of protein motifs and domains on the World Wide Web at sanger.ac.uk/Software/Pfam/ and pfam.janelia.org/. The information included at the Pfam database is described in Sonnhammer et al., Nucl. Acids Res., 26:320-322 (1998); Sonnhammer et al.,

Proteins, 28:405-420 (1997); and Bateman et al., Nucl. Acids Res., 27:260-262 (1999). Conserved regions also can be determined by aligning sequences of the same or related polypeptides from closely related species. Closely related species preferably are from the same family. In some embodiments, alignment of sequences from two different species is adequate.

Typically, polypeptides that exhibit at least about 40% amino acid sequence identity are useful to identify conserved regions. Conserved regions of related polypeptides exhibit at least 45% amino acid sequence identity (e.g., at least 50%, at

2018200459 19 Jan 2018 least 60%, at least 70%, at least 80%, or at least 90% amino acid sequence identity).

In some embodiments, a conserved region exhibits at least 92%, 94%, 96%, 98%, or

99% amino acid sequence identity.

For example, polypeptides suitable for producing steviol glycosides in a recombinant host include functional homologs of UGT91D2e, UGT91D2m,

UGT85C, and UGT76G. Such homologs have greater than 90% (e.g., at least 95% or 99%) sequence identity to the amino acid sequence of UGT91D2e (SEQ ID NO:5), UGT91D2m (SEQ ID NO: 10), UGT85C (SEQ ID NOG), or UGT76G (SEQ ID NOG). Variants of UGT91D2, UGT85C, and UGT76G polypeptides typically have

10 or fewer amino acid substitutions within the primary amino acid sequence, e.g., 7 or fewer amino acid substitutions, 5 or conservative amino acid substitutions, or between 1 and 5 substitutions. However, in some embodiments, variants of UGT91D2, UGT85C, and UGT76G polypeptides can have 10 or more amino acid substitutions (e.g., 10, 15, 20, 25, 30, 35, 10-20, 10-35, 20-30, or 25-35 amino acid substitutions). The substitutions may be conservative, or in some embodiments, nonconservative. Non-limiting examples of non-conservative changes in UGT91D2e polypeptides include glycine to arginine and tryptophan to arginine. Non-limiting examples of non-conservative substitutions in UGT76G polypeptides include valine to glutamic acid, glycine to glutamic acid, glutamine to alanine, and serine to proline.

Non-limiting examples of changes to UGT85C polypeptides include histidine to aspartic acid, proline to serine, lysine to threonine, and threonine to arginine.

In some embodiments, a useful UGT91D2 homolog can have amino acid substitutions (e.g., conservative amino acid substitutions) in regions of the polypeptide that are outside of predicted loops, e.g., residues 20-26, 39-43, 88-95,

1 21-124, 142-158, 185-198, and 203-214 are predicted loops in the N-terminal domain and residues 381-386 are predicted loops in the C-terminal domain of SEQ ID NOG. For example, a useful UGT91D2 homolog can include at least one amino acid substitution at residues 1-19, 27-38, 44-87, 96-120, 125-141, 159-184, 199-202, 215380, or 387-473 of SEQ ID NOG. In some embodiments, a UGT91D2 homolog can have an amino acid substitution at one or more residues selected from the group consisting of residues 30, 93, 99, 122, 140, 142, 148, 153, 156, 195, 196, 199, 206, 207, 211, 221, 286, 343, 427, and 438 of SEQ ID NOG. For example, a UGT91D2

2018200459 19 Jan 2018 functional homolog can have an amino acid substitution at one or more of residues

206, 207, and 343, such as an arginine at residue 206, a cysteine at residue 207, and an arginine at residue 343 of SEQ ID NO:5. See, SEQ ID NO:95. Other functional homologs of UGT91D2 can have one or more of the following: a tyrosine or phenylalanine at residue 30, a proline or glutamine at residue 93, a serine or valine at residue 99, a tyrosine or a phenylalanine at residue 122, a histidine or tyrosine at residue 140, a serine or cysteine at residue 142, an alanine or threonine at residue 148, a methionine at residue 152, an alanine at residue 153, an alanine or serine at residue 156, a glycine at residue 162, a leucine or methionine at residue 195, a glutamic acid at residue 196, a lysine or glutamic acid at residue 199, a leucine or methionine at residue 211, a leucine at residue 213, a serine or phenylalanine at residue 221, a valine or isoleucine at residue 253, a valine or alanine at residue 286, a lysine or asparagine at residue 427, an alanine at residue 438, and either an alanine or threonine at residue 462 of SEQ ID NO:5. See, Examples 11 and 16, and Tables 12 and 14. A useful variant UGT91D2 polypeptide also can be constructed based on the alignment set forth in Figure 8.

In some embodiments, a useful UGT85C homolog can have one or more amino acid substitutions at residues 9, 10, 13, 15, 21, 27, 60, 65, 71, 87, 91, 220, 243, 270, 289, 298, 334, 336, 350, 368, 389, 394, 397, 418, 420, 440, 441, 444, and 471 of

SEQ ID NO:3. Non-limiting examples of useful UGT85C homologs include polypeptides having substitutions (with respect to SEQ ID NO:3) at residue 65; at residue 65 in combination with residue 15, 270, 418, 440, or 441; residues 13, 15, 60, 270, 289, and 418; substitutions at residues 13, 60, and 270; substitutions at residues 60 and 87; substitutions at residues 65, 71, 220, 243, and 270; substitutions at residues

65, 71, 220, 243, 270, and 441; substitutions at residues 65, 71, 220, 389, and 394;

substitutions at residues 65, 71, 270, and 289; substitutions at residues 220, 243, 270, and 334; or substitutions at residues 270 and 289. See, Example 17 and Table 15.

In some embodiments, a useful UGT76G homolog can have one or more amino acid substitutions at residues 29, 74, 87, 91, 116, 123, 125, 126, 130, 145, 192,

1 93, 194, 196, 198, 199, 200, 203, 204, 205, 206, 207, 208, 266, 273, 274, 284, 285,

291, 330, 331, and 346 of SEQ ID NO:7. Non-limiting examples of useful UGT76G homologs include polypeptides having substitutions (with respect to SEQ ID NO:7) at

2018200459 19 Jan 2018 residues 74,87, 91, 116, 123, 125, 126, 130, 145, 192, 193, 194, 196, 198, 199, 200,

203, 204, 205, 206, 207, 208, and 291; residues 74,87, 91, 116, 123, 125, 126, 130,

145, 192, 193, 194, 196, 198, 199, 200, 203, 204, 205, 206, 207, 208, 266, 273, 274,

284, 285, and 291; or residues 74,87, 91, 116, 123, 125, 126, 130, 145, 192, 193,

194, 196, 198, 199, 200, 203, 204, 205, 206, 207, 208, 266, 273, 274, 284, 285, 291,

330, 331, and 346. See, Example 18 and Table 16.

Methods to modify the substrate specificity of, for example UGT91D2e, are known to those skilled in the art, and include without limitation site-directed/rational mutagenesis approaches, random directed evolution approaches and combinations in which random mutagenesis/saturation techniques are performed near the active site of the enzyme. For example see Sarah A. Osmani, et al. Phytochemistry 70 (2009) 325347.

A candidate sequence typically has a length that is from 80 percent to 200 percent of the length of the reference sequence, e.g., 82, 85, 87, 89, 90, 93, 95, 97, 99,

100, 105, 110, 115, 120, 130, 140, 150, 160, 170, 180, 190, or 200 percent of the length of the reference sequence. A percent identity for any candidate nucleic acid or polypeptide relative to a reference nucleic acid or polypeptide can be determined as follows. A reference sequence (e.g., a nucleic acid sequence or an amino acid sequence) is aligned to one or more candidate sequences using the computer program

ClustalW (version 1.83, default parameters), which allows alignments of nucleic acid or polypeptide sequences to be carried out across their entire length (global alignment). Chenna et al., Nucleic Acids Res., 31(13):3497-500 (2003).

ClustalW calculates the best match between a reference and one or more candidate sequences, and aligns them so that identities, similarities and differences can be determined. Gaps of one or more residues can be inserted into a reference sequence, a candidate sequence, or both, to maximize sequence alignments. For fast pairwise alignment of nucleic acid sequences, the following default parameters are used: word size: 2; window size: 4; scoring method: percentage; number of top diagonals: 4; and gap penalty: 5. For multiple alignment of nucleic acid sequences, the following parameters are used: gap opening penalty: 10.0; gap extension penalty: 5.0; and weight transitions: yes. For fast pairwise alignment of protein sequences, the following parameters are used: word size: 1; window size: 5; scoring method:

2018200459 19 Jan 2018 percentage; number of top diagonals: 5; gap penalty: 3. For multiple alignment of protein sequences, the following parameters are used: weight matrix: blosum; gap opening penalty: 10.0; gap extension penalty: 0.05; hydrophilic gaps: on; hydrophilic residues: Gly, Pro, Ser, Asn, Asp, Gln, Glu, Arg, and Lys; residue-specific gap penalties: on. The ClustalW output is a sequence alignment that reflects the relationship between sequences. ClustalW can be run, for example, at the Baylor College of Medicine Search Launcher site on the World Wide Web (searchlauncher.bcm.tmc.edu/multi-align/multi-align.html) and at the European Bioinformatics Institute site on the World Wide Web (ebi.ac.uk/clustalw).

To determine percent identity of a candidate nucleic acid or amino acid sequence to a reference sequence, the sequences are aligned using ClustalW, the number of identical matches in the alignment is divided by the length of the reference sequence, and the result is multiplied by 100. It is noted that the percent identity value can be rounded to the nearest tenth. For example, 78.11, 78.12, 78.13, and

78.14 are rounded down to 78.1, while 78.15, 78.16, 78.17, 78.18, and 78.19 are rounded up to 78.2.

It will be appreciated that a functional UGT91D2 polypeptide can include additional amino acids that are not involved in glucosylation or other enzymatic activities carried out by UGT91D2, and thus such a polypeptide can be longer than would otherwise be the case. For example, a UGT91D2 polypeptide can include a purification tag, a chloroplast transit peptide, a mitochondrial transit peptide, an amyloplast peptide, signal peptide, or a secretion tag added to the amino or carboxy terminus. In some embodiments, aUGT91D2 polypeptide includes an amino acid sequence that functions as a reporter, e.g., a green fluorescent protein or yellow fluorescent protein.

II. Steviol and Steviol Glycoside Biosynthesis Nucleic Acids

A recombinant gene encoding a polypeptide described herein comprises the coding sequence for that polypeptide, operably linked in sense orientation to one or more regulatory regions suitable for expressing the polypeptide. Because many microorganisms are capable of expressing multiple gene products from a polycistronic mRNA, multiple polypeptides can be expressed under the control of a single

2018200459 19 Jan 2018 regulatory region for those microorganisms, if desired. A coding sequence and a regulatory region are considered to be operably linked when the regulatory region and coding sequence are positioned so that the regulatory region is effective for regulating transcription or translation of the sequence. Typically, the translation initiation site of the translational reading frame of the coding sequence is positioned between one and about fifty nucleotides downstream of the regulatory region for a monocistronic gene.

In many cases, the coding sequence for a polypeptide described herein is identified in a species other than the recombinant host, i.e., is a heterologous nucleic acid. Thus, if the recombinant host is a microorganism, the coding sequence can be from other prokaryotic or eukaryotic microorganisms, from plants or from animals.

In some case, however, the coding sequence is a sequence that is native to the host and is being reintroduced into that organism. A native sequence can often be distinguished from the naturally occurring sequence by the presence of non-natural sequences linked to the exogenous nucleic acid, e.g., non-native regulatory sequences flanking a native sequence in a recombinant nucleic acid construct. In addition, stably transformed exogenous nucleic acids typically are integrated at positions other than the position where the native sequence is found.

“Regulatory region” refers to a nucleic acid having nucleotide sequences that influence transcription or translation initiation and rate, and stability and/or mobility of a transcription or translation product. Regulatory regions include, without limitation, promoter sequences, enhancer sequences, response elements, protein recognition sites, inducible elements, protein binding sequences, 5' and 3' untranslated regions (UTRs), transcriptional start sites, termination sequences, polyadenylation sequences, introns, and combinations thereof. A regulatory region typically comprises at least a core (basal) promoter. A regulatory region also may include at least one control element, such as an enhancer sequence, an upstream element or an upstream activation region (UAR). A regulatory region is operably linked to a coding sequence by positioning the regulatory region and the coding sequence so that the regulatory region is effective for regulating transcription or translation of the sequence. For example, to operably link a coding sequence and a promoter sequence, the translation initiation site of the translational reading frame of the coding sequence is typically positioned between one and about fifty nucleotides

2018200459 19 Jan 2018 downstream of the promoter. A regulatory region can, however, be positioned as much as about 5,000 nucleotides upstream of the translation initiation site, or about

2,000 nucleotides upstream of the transcription start site.

The choice of regulatory regions to be included depends upon several factors, including, but not limited to, efficiency, selectability, inducibility, desired expression level, and preferential expression during certain culture stages. It is a routine matter for one of skill in the art to modulate the expression of a coding sequence by appropriately selecting and positioning regulatory regions relative to the coding sequence. It will be understood that more than one regulatory region may be present,

e.g., introns, enhancers, upstream activation regions, transcription terminators, and inducible elements.

One or more genes can be combined in a recombinant nucleic acid construct in “modules” useful for a discrete aspect of steviol and/or steviol glycoside production. Combining a plurality of genes in a module, particularly a polycistronic module, facilitates the use of the module in a variety of species. For example, a steviol biosynthesis gene cluster, or a UGT gene cluster, can be combined in a polycistronic module such that, after insertion of a suitable regulatory region, the module can be introduced into a wide variety of species. As another example, a UGT gene cluster can be combined such that each UGT coding sequence is operably linked to a separate regulatory region, to form a UGT module. Such a module can be used in those species for which monocistronic expression is necessary or desirable. In addition to genes useful for steviol or steviol glycoside production, a recombinant construct typically also contains an origin of replication, and one or more selectable markers for maintenance of the construct in appropriate species.

It will be appreciated that because of the degeneracy of the genetic code, a number of nucleic acids can encode a particular polypeptide; i.e., for many amino acids, there is more than one nucleotide triplet that serves as the codon for the amino acid. Thus, codons in the coding sequence for a given polypeptide can be modified such that optimal expression in a particular host is obtained, using appropriate codon bias tables for that host (e.g., microorganism). SEQ ID NOs: 18-25, 34-36, 40-43, 4849, 52-55, 60-64, and 70-72 set forth nucleotide sequences encoding certain enzymes for steviol and steviol glycoside biosynthesis, modified for increased expression in

2018200459 19 Jan 2018 yeast. As isolated nucleic acids, these modified sequences can exist as purified molecules and can be incorporated into a vector or a virus for use in constructing modules for recombinant nucleic acid constructs.

In some cases, it is desirable to inhibit one or more functions of an endogenous polypeptide in order to divert metabolic intermediates towards steviol or steviol glycoside biosynthesis. For example, it may be desirable to downregulate synthesis of sterols in a yeast strain in order to further increase steviol or steviol glycoside production, e.g., by downregulating squalene epoxidase. As another example, it may be desirable to inhibit degradative functions of certain endogenous gene products, e.g., glycohydrolases that remove glucose moieties from secondary metabolites. As another example, expression of membrane transporters involved in transport of steviol glycosides can be inhibited, such that secretion of glycosylated steviosides is inhibited. Such regulation can be beneficial in that secretion of steviol glycosides can be inhibited for a desired period of time during culture of the microorganism, thereby increasing the yield of glycoside product(s) at harvest. In such cases, a nucleic acid that inhibits expression of the polypeptide or gene product may be included in a recombinant construct that is transformed into the strain. Alternatively, mutagenesis can be used to generate mutants in genes for which it is desired to inhibit function.

III. Hosts

A. Microorganisms

A number of prokaryotes and eukaryotes are suitable for use in constructing the recombinant microorganisms described herein, e.g., gram-negative bacteria, yeast and fungi. A species and strain selected for use as a steviol or steviol glycoside production strain is first analyzed to determine which production genes are endogenous to the strain and which genes are not present. Genes for which an endogenous counterpart is not present in the strain are assembled in one or more recombinant constructs, which are then transformed into the strain in order to supply the missing function(s).

Exemplary prokaryotic and eukaryotic species are described in more detail below. However, it will be appreciated that other species may be suitable. For

2018200459 19 Jan 2018 example, suitable species may be in a genus selected from the group consisting of

Agaricus, Aspergillus, Bacillus, Candida, Corynebacterium, Escherichia,

Fusarium! Gibberella, Kluyveromyces, Laetiporus, Lentinus, Phaffia, Phanerochaete,

Pichia, Physcomitrella, Rhodoturula, Saccharomyces, Schizosaccharomyces,

Sphaceloma, Xanthophyllomyces and Yarrowia. Exemplary species from such genera include Lentinus tigrinus, Laetiporus sulphureus, Phanerochaete chrysosporium, Pichiapastoris, Physcomitrellapatens, Rhodoturula glutinis 32, Rhodoturula mucilaginosa, Phaffia rhodozyma UBV-AX, Xanthophyllomyces dendrorhous, Fusarium fujikuroi!Gibberella fujikuroi, Candida utilis and Yarrowia lipolytica. In o some embodiments, a microorganism can be an Ascomycete such as Gibberella fujikuroi, Kluyveromyces lactis, Schizosaccharomyces pombe, Aspergillus niger, or Saccharomyces cerevisiae. In some embodiments, a microorganism can be a prokaryote such as Escherichia coli, Rhodobacter sphaeroides, or Rhodobacter capsulatus. It will be appreciated that certain microorganisms can be used to screen and test genes of interest in a high throughput manner, while other microorganisms with desired productivity or growth characteristics can be used for large-scale production of steviol glycosides.

Saccharomyces cerevisiae

Saccharomyces cerevisiae is a widely used chassis organism in synthetic biology, and can be used as the recombinant microorganism platform. There are libraries of mutants, plasmids, detailed computer models of metabolism and other information available for S. cerevisiae, allowing for rational design of various modules to enhance product yield. Methods are known for making recombinant microorganisms.

A steviol biosynthesis gene cluster can be expressed in yeast using any of a number of known promoters. Strains that overproduce terpenes are known and can be used to increase the amount of geranylgeranyl diphosphate available for steviol and steviol glycoside production.

Aspergillus sr>r>.

Aspergillus species such as A. oryzae, A. niger and A. sojae are widely used microorganisms in food production, and can also be used as the recombinant microorganism platform. Nucleotide sequences are available for genomes of 4.

2018200459 19 Jan 2018 nidulans, A.fumigatus, A. oryzae, A. clavatus, A.flavus, A. niger, and A. terreus, allowing rational design and modification of endogenous pathways to enhance flux and increase product yield. Metabolic models have been developed for Aspergillus, as well as transcriptomic studies and proteomics studies. A. niger is cultured for the industrial production of a number of food ingredients such as citric acid and gluconic acid, and thus species such as A. niger are generally suitable for the production of food ingredients such as steviol and steviol glycosides. Example 23 describes cloning methodology for production of steviol glycosides in Aspergillus nidulans.

Escherichia coli

Escherichia coli, another widely used platform organism in synthetic biology, can also be used as the recombinant microorganism platform. Similar to Saccharomyces, there are libraries of mutants, plasmids, detailed computer models of metabolism and other information available for E. coli, allowing for rational design of various modules to enhance product yield. Methods similar to those described above for Saccharomyces can be used to make recombinant E. coli microorganisms. Agaricus, Gihherella. and Phanerochaete spp.

Agaricus, Gibberella, and Phanerochaete spp. can be useful because they are known to produce large amounts of gibberellin in culture. Thus, the terpene precursors for producing large amounts of steviol and steviol glycosides are already produced by endogenous genes. Thus, modules containing recombinant genes for steviol or steviol glycoside biosynthesis polypeptides can be introduced into species from such genera without the necessity of introducing mevalonate or MEP pathway genes.

Rhodobacter spp.

Rhodobacter can be use as the recombinant microorganism platform. Similar to E. coli, there are libraries of mutants available as well as suitable plasmid vectors, allowing for rational design of various modules to enhance product yield. Isoprenoid pathways have been engineered in membraneous bacterial species of Rhodobacter for increased production of carotenoid and CoQlO. See, U.S. Patent Publication Nos.

20050003474 and 20040078846. Methods similar to those described above for E. coli can be used to make recombinant Rhodobacter microorganisms.

Physcomitrella spp.

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Physcomitrella mosses, when grown in suspension culture, have characteristics similar to yeast or other fungal cultures. This genera is becoming an important type of cell for production of plant secondary metabolites, which can be difficult to produce in other types of cells. Example 22 describes production of active

UGT enzymes in the steviol glycoside pathway in P. patens.

B. Plant Cells or Plants

In some embodiments, the nucleic acids and polypeptides described herein are introduced into plants or plant cells to increase overall steviol glycoside production or enrich for the production of specific steviol glycosides in proportion to others. Thus, a host can be a plant or a plant cell that includes at least one recombinant gene described herein. A plant or plant cell can be transformed by having a recombinant gene integrated into its genome, i.e., can be stably transformed. Stably transformed cells typically retain the introduced nucleic acid with each cell division. A plant or plant cell can also be transiently transformed such that the recombinant gene is not integrated into its genome. Transiently transformed cells typically lose all or some portion of the introduced nucleic acid with each cell division such that the introduced nucleic acid cannot be detected in daughter cells after a sufficient number of cell divisions. Both transiently transformed and stably transformed transgenic plants and plant cells can be useful in the methods described herein.

Transgenic plant cells used in methods described herein can constitute part or all of a whole plant. Such plants can be grown in a manner suitable for the species under consideration, either in a growth chamber, a greenhouse, or in a field. Transgenic plants can be bred as desired for a particular purpose, e.g., to introduce a recombinant nucleic acid into other lines, to transfer a recombinant nucleic acid to other species, or for further selection of other desirable traits. Alternatively, transgenic plants can be propagated vegetatively for those species amenable to such techniques. As used herein, a transgenic plant also refers to progeny of an initial transgenic plant provided the progeny inherits the transgene. Seeds produced by a transgenic plant can be grown and then selfed (or outcrossed and selfed) to obtain seeds homozygous for the nucleic acid construct.

2018200459 19 Jan 2018

Transgenic plants can be grown in suspension culture, or tissue or organ culture. For the purposes of this invention, solid and/or liquid tissue culture techniques can be used. When using solid medium, transgenic plant cells can be placed directly onto the medium or can be placed onto a fdter that is then placed in contact with the medium. When using liquid medium, transgenic plant cells can be placed onto a flotation device, e.g., a porous membrane that contacts the liquid medium.

When transiently transformed plant cells are used, a reporter sequence encoding a reporter polypeptide having a reporter activity can be included in the transformation procedure and an assay for reporter activity or expression can be performed at a suitable time after transformation. A suitable time for conducting the assay typically is about 1-21 days after transformation, e.g., about 1-14 days, about 17 days, or about 1-3 days. The use of transient assays is particularly convenient for rapid analysis in different species, or to confirm expression of a heterologous polypeptide whose expression has not previously been confirmed in particular recipient cells.

Techniques for introducing nucleic acids into monocotyledonous and dicotyledonous plants are known in the art, and include, without limitation, Agmhactemtm-mcdvdicd transformation, viral vector-mediated transformation, electroporation and particle gun transformation, U.S. Patent Nos 5,538,880;

5,204,253; 6,329,571; and 6,013,863. If a cell or cultured tissue is used as the recipient tissue for transformation, plants can be regenerated from transformed cultures if desired, by techniques known to those skilled in the art.

A population of transgenic plants can be screened and/or selected for those members of the population that have a trait or phenotype conferred by expression of the transgene. For example, a population of progeny of a single transformation event can be screened for those plants having a desired level of expression of a steviol or steviol glycoside biosynthesis polypeptide or nucleic acid. Physical and biochemical methods can be used to identify expression levels. These include Southern analysis or

PCR amplification for detection of a polynucleotide; Northern blots, St RNase protection, primer-extension, or RT-PCR amplification for detecting RNA transcripts; enzymatic assays for detecting enzyme or ribozyme activity of polypeptides and

2018200459 19 Jan 2018 polynucleotides; and protein gel electrophoresis, Western blots, immunoprecipitation, and enzyme-linked immunoassays to detect polypeptides. Other techniques such as in situ hybridization, enzyme staining, and immunostaining also can be used to detect the presence or expression of polypeptides and/or nucleic acids. Methods for performing all of the referenced techniques are known. As an alternative, a population of plants comprising independent transformation events can be screened for those plants having a desired trait, such as production of a steviol glycoside or modulated biosynthesis of a steviol glycoside. Selection and/or screening can be carried out over one or more generations, and/or in more than one geographic location. In some cases, transgenic plants can be grown and selected under conditions which induce a desired phenotype or are otherwise necessary to produce a desired phenotype in a transgenic plant. In addition, selection and/or screening can be applied during a particular developmental stage in which the phenotype is expected to be exhibited by the plant. Selection and/or screening can be carried out to choose those transgenic plants having a statistically significant difference in a steviol glycoside level relative to a control plant that lacks the transgene.

The nucleic acids, recombinant genes, and constructs described herein can be used to transform a number of monocotyledonous and dicotyledonous plants and plant cell systems. Non-limiting examples of suitable monocots include, for example, cereal crops such as rice, rye, sorghum, millet, wheat, maize, and barley. The plant may be a non-cereal monocot such as asparagus, banana, or onion. The plant also may be a dicot such as stevia (Stevia rebaudiana), soybean, cotton, sunflower, pea, geranium, spinach, or tobacco. In some cases, the plant may contain the precursor pathways for phenyl phosphate production such as the mevalonate pathway, typically found in the cytoplasm and mitochondria. The non-mevalonate pathway is more often found in plant plastids [Dubey, et al., 2003 J. Biosci. 28 637-646], One with skill in the art may target expression of steviol glycoside biosynthesis polypeptides to the appropriate organelle through the use of leader sequences, such that steviol glycoside biosynthesis occurs in the desired location of the plant cell. One with skill in the art will use appropriate promoters to direct synthesis, e.g., to the leaf of a plant, if so desired. Expression may also occur in tissue cultures such as callus culture or hairy root culture, if so desired.

2018200459 19 Jan 2018

In one embodiment, one or more nucleic acid or polypeptides described herein are introduced into Stevia (e.g., Stevia rebaudiana) such that overall steviol glycoside biosynthesis is increased or that the the overall steviol glycoside composition is selectively enriched for one or more specific steviol glycosides. For example, one or more recombinant genes can be introduced into Stevia such that one or more of the following are expressed: a UGT91D enzyme such as UGT91D2e (e.g., SEQ ID NO:5 or a functional homolog thereof), UGT91D2m (e.g., SEQ fD NO: 10); a UGT85C enzyme such as a variant set forth in Table 15, or a UGT76G1 enzyme such as a variant set forth in Example 18. Nucleic acid constructs typically include a suitable promoter (e.g., 35S, e35S, or ssRUBfSCO promoters) operably linked to a nucleic acid encoding the UGT polypeptide. Nucleic acids can be introduced into Stevia by Agrohacteriiim-mcdvdtcd transformation; electroporation-mediated gene transfer to protoplasts; or by particle bombardment. See, e.g., Singh, et al., Compendium of Transgenic Crop Plants: Transgenic Sugar, Tuber and Fiber, Edited by Chittaranjan

Kole and Timothy C. Hall, Blackwell Publishing Etd. (2008), pp. 97-115. For particle bombardment of stevia leaf derived callus, the parameters can be as follows: 6 cm distance, 1100 psi He pressure, gold particles, and one bombardment.

Stevia plants can be regenerated by somatic embryogenesis as described by Singh et al., 2008, supra, in particular, leaf segments (approximately l-2cm long) can be removed from 5 to 6-week-old in vitro raised plants and incubated (adaxial side down) on MS medium supplemented with B5 vitamins, 30 g sucrose and 3 g Gelrite. 2,4-dichlorophenoxyacetic acid (2,4-D) can be used in combination with 6-benzyl adenine (BA), kinetin (KN), or zeatin. Proembryogenic masses appear after 8 weeks of subculture. Within 2-3 weeks of subcultures, somatic embryos will appear on the surface of cultures. Embryos can be matured in medium containing BA in combination with 2,4-D, a-naphthaleneacetic acid (NAA), or indolbutyric acid (IBA). Mature somatic embryos that germinate and form plantlets can be excised from calli. After plantlets reach 3-4 weeks, the plantlets can be transferred to pots with vermiculite and grown for 6-8 weeks in growth chambers for acclimatization and transferred to greenhouses.

In one embodiment, steviol glycosides are produced in rice. Rice and maize are readily transformable using techniques such as Agrobacterium-mQddaiQd

2018200459 19 Jan 2018 transformation. Binary vector systems are commonly utilized for Agrobacterium exogenous gene introduction to monocots. See, for example, U.S. Patent Nos.

6,215,051 and 6,329,571. In a binary vector system, one vector contains the T-DNA region, which includes a gene of interest (e.g., a UGT described herein) and the other vector is a disarmed Ti plasmid containing the vir region. Co-integrated vectors and mobilizable vectors also can be used. The types and pretreatment of tissues to be transformed, the strain of Agrobacterium used, the duration of the inoculation, the prevention of overgrowth and necrosis by the Agrobacterium, can be readily adjusted by one of skill in the art. Immature embryo cells of rice can be prepared for transformation with Agrobacterium using binary vectors. The culture medium used is supplemented with phenolic compounds. Alternatively, the transformation can be done inplanta using vacuum infdtration. See, for example, WO 2000037663, WO 2000063400, and WO 2001012828.

IV. Methods of Producing Steviol and Steviol Glycosides

Recombinant hosts described herein can be used in methods to produce steviol or steviol glycosides. For example, if the recombinant host is a microorganism, the method can include growing the recombinant microorganism in a culture medium under conditions in which steviol and/or steviol glycoside biosynthesis genes are expressed. The recombinant microorganism may be grown in a fed batch or continuous process. Typically, the recombinant microorganism is grown in a fermentor at a defined temperature(s) for a desired period of time. Depending on the particular microorganism used in the method, other recombinant genes such as isopentenyl biosynthesis genes and terpene synthase and cyclase genes may also be present and expressed. Levels of substrates and intermediates, e.g., isopentenyl diphosphate, dimethylallyl diphosphate, geranylgeranyl diphosphate, kaurene and kaurenoic acid, can be determined by extracting samples from culture media for analysis according to published methods.

After the recombinant microorganism has been grown in culture for the desired period of time, steviol and/or one or more steviol glycosides can then be recovered from the culture using various techniques known in the art. If the recombinant host is a plant or plant cells, steviol or steviol glycosides can be extracted

2018200459 19 Jan 2018 from the plant tissue using various techniques known in the art. For example, a crude lysate of the cultured microorganism or plant tissue can be centrifuged to obtain a supernatant. The resulting supernatant can then be applied to a chromatography column, e.g., a C-18 column, and washed with water to remove hydrophilic compounds, followed by elution of the compound(s) of interest with a solvent such as methanol. The compound(s) can then be further purified by preparative HPLC. See also WO 2009/140394.

The amount of steviol or steviol glycoside produced can be from about 1 mg/1 to about 1,500 mg/1, e.g., about 1 to about 10 mg/1, about 3 to about 10 mg/1, about 5 to about 20 mg/1, about 10 to about 50 mg/1, about 10 to about 100 mg/1, about 25 to about 500 mg/1, about 100 to about 1,500 mg/1, or about 200 to about 1,000 mg/1. In general, longer culture times will lead to greater amounts of product. Thus, the recombinant microorganism can be cultured for from 1 day to 7 days, from 1 day to 5 days, from 3 days to 5 days, about 3 days, about 4 days, or about 5 days.

It will be appreciated that the various genes and modules discussed herein can be present in two or more recombinant microorganisms rather than a single microorganism. When a plurality of recombinant microorganisms is used, they can be grown in a mixed culture to produce steviol and/or steviol glycosides. For example, a first microorganism can comprise one or more biosynthesis genes for producing steviol while a second microorganism comprises steviol glycoside biosynthesis genes. Alternatively, the two or more microorganisms each can be grown in a separate culture medium and the product of the first culture medium, e.g., steviol, can be introduced into second culture medium to be converted into a subsequent intermediate, or into an end product such as rebaudioside A. The product produced by the second, or final microorganism is then recovered. It will also be appreciated that in some embodiments, a recombinant microorganism is grown using nutrient sources other than a culture medium and utilizing a system other than a fermentor.

Steviol glycosides do not necessarily have equivalent performance in different food systems. It is therefore desirable to have the ability to direct the synthesis to steviol glycoside compositions of choice. Recombinant hosts described herein can produce compositions that are selectively enriched for specific steviol glycosides and have a consistent taste profile. Thus, the recombinant microorganisms, plants, and

2018200459 19 Jan 2018 plant cells described herein can facilitate the production of compositions that are tailored to meet the sweetening profile desired for a given food product and that have a proportion of each steviol glycoside that is consistent from batch to batch.

Microorganisms described herein do not produce the undesired plant byproducts found in Stevia extracts. Thus, steviol glycoside compositions produced by the recombinant microorganisms described herein are distinguishable from compositions derived from Stevia plants.

V. Food Products

The steviol and steviol glycosides obtained by the methods disclosed herein can be used to make food products, dietary supplements and sweetener compositions. For example, substantially pure steviol or steviol glycoside such as rebaudioside A can be included in food products such as ice cream, carbonated beverages, fruit juices, yogurts, baked goods, chewing gums, hard and soft candies, and sauces. Substantially pure steviol or steviol glycoside can also be included in non-food products such as pharmaceutical products, medicinal products, dietary supplements and nutritional supplements. Substantially pure steviol or steviol glycosides may also be included in animal feed products for both the agriculture industry and the companion animal industry. Alternatively, a mixture of steviol and/or steviol glycosides can be made by culturing recombinant microorganisms separately or growing different plants/plant cells, each producing a specific steviol or steviol glycoside, recovering the steviol or steviol glycoside in substantially pure form from each microorganism or plant/plant cells and then combining the compounds to obtain a mixture containing each compound in the desired proportion. The recombinant microorganisms, plants, and plant cells described herein permit more precise and consistent mixtures to be obtained compared to current Stevia products. In another alternative, a substantially pure steviol or steviol glycoside can be incorporated into a food product along with other sweeteners, e.g. saccharin, dextrose, sucrose, fructose, erythritol, aspartame, sucralose, monatin, or acesulfame potassium. The weight ratio of steviol or steviol glycoside relative to other sweeteners can be varied as desired to achieve a satisfactory taste in the final food product. See, e.g., U.S. Patent Publication No. 2007/0128311. In some embodiments, the steviol or steviol glycoside may be

2018200459 19 Jan 2018 provided with a flavor (e.g., citrus) as a flavor modulator. For example, Rebaudioside

C can be used as a sweetness enhancer or sweetness modulator, in particular for carbohydrate based sweeteners, such that the amount of sugar can be reduced in the food product.

Compositions produced by a recombinant microorganism, plant, or plant cell described herein can be incorporated into food products. For example, a steviol glycoside composition produced by a recombinant microorganism, plant, or plant cell can be incorporated into a food product in an amount ranging from about 20 mg steviol glycoside/kg food product to about 1800 mg steviol glycoside/kg food product on a dry weight basis, depending on the type of steviol glycoside and food product. For example, a steviol glycoside composition produced by a recombinant microorganism, plant, or plant cell can be incorporated into a dessert, cold confectionary (e.g., ice cream), dairy product (e.g., yogurt), or beverage (e.g., a carbonated beverage) such that the food product has a maximum of 500 mg steviol glycoside/kg food on a dry weight basis. A steviol glycoside composition produced by a recombinant microorganism, plant, or plant cell can be incorporated into a baked good (e.g., a biscuit) such that the food product has a maximum of 300 mg steviol glycoside/kg food on a dry weight basis. A steviol glycoside composition produced by a recombinant microorganism, plant, or plant cell can be incorporated into a sauce (e.g., chocolate syrup) or vegetable product (e.g., pickles) such that the food product has a maximum of 1000 mg steviol glycoside/kg food on a dry weight basis. A steviol glycoside composition produced by a recombinant microorganism, plant, or plant cell can be incorporated into a bread such that the food product has a maximum of 160 mg steviol glycoside/kg food on a dry weight basis. A steviol glycoside composition produced by a recombinant microorganism, plant, or plant cell can be incorporated into a hard or soft candy such that the food product has a maximum of 1600 mg steviol glycoside/kg food on a dry weight basis. A steviol glycoside composition produced by a recombinant microorganism, plant, or plant cell can be incorporated into a processed fruit product (e.g., fruit juices, fruit idling, jams, and jellies) such that the food product has a maximum of 1000 mg steviol glycoside/kg food on a dry weight basis.

2018200459 19 Jan 2018

For example, such a steviol glycoside composition can have from 90-99% rebaudioside A and an undetectable amount of stevia plant-derived contaminants, and be incorporated into a food product at from 25-1600 mg/kg, e.g., 100-500 mg/kg, 25100 mg/kg, 250-1000 mg/kg, 50-500 mg/kg or 500-1000 mg/kg on a dry weight basis.

Such a steviol glycoside composition can be a rebaudioside B-enriched composition having greater than 3% rebaudioside B and be incorporated into the food product such that the amount of rebaudioside B in the product is from 25-1600 mg/kg, e.g., 100-500 mg/kg, 25-100 mg/kg, 250-1000 mg/kg, 50-500 mg/kg or 500-1000 mg/kg on a dry weight basis. Typically, the rebaudioside B-enriched composition has an undetectable amount of stevia plant-derived contaminants.

Such a steviol glycoside composition can be a rebaudioside C-enriched composition having greater than 15% rebaudioside C and be incorporated into the food product such that the amount of rebaudioside C in the product is from 20-600 mg/kg, e.g., 100-600 mg/kg, 20-100 mg/kg, 20-95 mg/kg, 20-250 mg/kg, 50-75 mg/kg or 50-95 mg/kg on a dry weight basis. Typically, the rebaudioside C-enriched composition has an undetectable amount of stevia plant-derived contaminants.

Such a steviol glycoside composition can be a rebaudioside D-enriched composition having greater than 3% rebaudioside D and be incorporated into the food product such that the amount of rebaudioside D in the product is from 25-1600 mg/kg,

e.g., 100-500 mg/kg, 25-100 mg/kg, 250-1000 mg/kg, 50-500 mg/kg or 500-1000 mg/kg on a dry weight basis. Typically, the rebaudioside D-enriched composition has an undetectable amount of stevia plant-derived contaminants.

Such a steviol glycoside composition can be a rebaudioside E-enriched composition having greater than 3% rebaudioside E and be incorporated into the food product such that the amount of rebaudioside E in the product is from 25-1600 mg/kg, e.g., 100-500 mg/kg, 25-100 mg/kg, 250-1000 mg/kg, 50-500 mg/kg or 500-1000 mg/kg on a dry weight basis. Typically, the rebaudioside E-enriched composition has an undetectable amount of stevia plant-derived contaminants.

Such a steviol glycoside composition can be a rebaudioside F-enriched composition having greater than 4% rebaudioside F and be incorporated into the food product such that the amount of rebaudioside F in the product is from 25-1000 mg/kg, e.g., 100-600 mg/kg, 25-100 mg/kg, 25-95 mg/kg, 50-75 mg/kg or 50-95 mg/kg on a

2018200459 19 Jan 2018 dry weight basis. Typically, the rebaudioside F-enriched composition has an undetectable amount of stevia plant-derived contaminants.

Such a steviol glycoside composition can be a dulcoside A-enriched composition having greater than 4% dulcoside A and be incorporated into the food product such that the amount of dulcoside A in the product is from 25-1000 mg/kg, e.g., 100-600 mg/kg, 25-100 mg/kg, 25-95 mg/kg, 50-75 mg/kg or 50-95 mg/kg on a dry weight basis. Typically, the dulcoside A-enriched composition has an undetectable amount of stevia plant-derived contaminants.

In some embodiments, a substantially pure steviol or steviol glycoside is incorporated into a tabletop sweetener or “cup-for-cup” product. Such products typically are diluted to the appropriate sweetness level with one or more bulking agents, e.g., maltodextrins, known to those skilled in the art. Steviol glycoside compositions enriched for rebaudioside A, rebaudioside C, rebaudioside D, rebaudioside E, rebaudioside F, or dulcoside A can be package in a sachet, for example, at from 10,000 to 30,000 mg steviol glycoside/kg product on a dry weight basis, for tabletop use.

VI. Plant Breeding

A. Polymorphisms

Polymorphisms among the nucleic acids described herein (e.g., UGT91D2 nucleic acids) can be used as markers in plant genetic mapping and plant breeding programs in Stevia. See, e.g., Yao et al., Genome, 1999, 42:657-661. Thus, the polymorphisms described herein can be used in a method of identifying whether that polymorphism is associated with variation in a trait. The method involves measuring the correlation between variation in the trait in plants of a Stevia line or population and the presence of one or more genetic polymorphisms in those plants, thereby identifying whether or not the genetic polymorphisms are associated with variation in the trait. Typically, the trait is the total amount of steviol glycosides present in leaves of the plant, although the trait also can be the amount of a particular steviol glycoside,

e.g., rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, rebaudioside F, or dulcoside A. In some embodiments, the trait is the amount of steviol, or the amount of an isoprenoid precursor. A statistically significant correlation

2018200459 19 Jan 2018 between the trait and the presence of the polymorphic marker is determined using an appropriate parametric or non-parametric statistic, e.g., Chi-square test, Student's ttest, Mann-Whitney test, or F-test. A statistically significant correlation between, for example, the amount of rebaudioside A in a plant and presence of a polymorphic marker indicates that the marker may be useful in a marker-assisted breeding program for selection of altered rebaudioside A levels.

Polymorphisms may be detected by means known in the art, including without limitation, restriction fragment length polymorphism (RFFP), random amplified polymorphic DNA detection (RAPD), amplified fragment length polymorphism (AFFP), simple sequence repeat (SSR) or microsatellites. Discovery, detection, and genotyping of polymorphisms have been described in the literature. See, e.g., Henry, ed. (2001) Plant Genotyping. The DNA Fingerprinting of Plants Wallingford: CABI Publishing; and Phillips and Vasil, eds. (2001) DNA-based Markers in Plants Dordrecht: Kluwer Academic Publishers. For example, a primer or probe derived from the nucleic acid sequences set forth in SEQ ID NOG, SEQ ID NO:9, or SEQ ID NO:96, or the complements thereof, can be used to identify one or more individual plants that possess the polymorphic allele that is correlated with a desired steviol glycoside composition. Those plants then can be used in a breeding program to combine the polymorphic allele with a plurality of other alleles at other loci that are correlated with the desired steviol glycoside composition. As will be evident to one of skill, the number and type of markers required can differ, depending on the trait(s) to be selected for and the degree of correlation for each marker. The methods, therefore, involve detecting a plurality of polymorphisms in the genome of the plant in certain embodiments. It will be appreciated that the method may further comprise storing the results of the step of detecting the plurality of polymorphisms on a computer readable medium.

Thus, in some embodiments, a method for identifying Stevia plant lines or populations comprises supplying a nucleic acid sample for a Stevia plant, providing amplification primers for amplifying a region of a Stevia plant corresponding to a

UGT gene having 90% or greater sequence identity to a nucleic acid encoding the polypeptides set forth inSEQ ID NOs: 1, 3, 5, or 7, present in the sample, applying the amplification primers to the nucleic acid sample such that amplification of the region

2018200459 19 Jan 2018 occurs, and identifying plants having a desired trait based on the presence of one or more polymorphisms in the amplified nucleic acid sample that correlate with the trait.

In some embodiments, a method of determining the presence of a polynucleotide in a Stevia plant involves contacting at least one probe or primer pair with nucleic acid from the plant. The probe or primer pair is specific for a polynucleotide that encodes a UGT polypeptide having at least 90% sequence identity to SEQ ID NOs: 1,3,5, or 7. The presence or absence of the polynucleotide is then determined.

In addition to methods for detecting polymorphisms and determining the genotype of a Stevia plant, kits suitable for carrying out the methods are also described, as well as a computer readable medium produced by such methods that contains data generated by the methods. A kit for genotyping a Stevia biological sample includes a primer pair that specifically amplifies, or a probe that specifically hybridizes to, a polynucleotide that encodes a UGT polypeptide having at least 90% sequence identity to SEQ ID NOs: 1, 3, 5, or 7. Such kits typically have the primer or probe contained within suitable packaging material.

In some embodiments of the methods and kits described herein, one or more sets of oligonucleotides, each capable of recognizing the presence or absence of a specific and defined genomic position, is used. For polyploid Stevia lines or populations, more oligonucleotides are desirable. The lower limit is one oligonucleotide pair and the upper limit is set by the desired resolution capacity of the method and the test kit. Hybridization of the oligonucleotides to DNA from the Stevia plant is preferably recorded in situ by any conventional labelling system, applying for instance terminal transferase and conventional recordable labels. As an alternative to in situ labelling the hybridized sample DNA may be released from the solid support and subsequently hybridized with labelled polynucleotide sequences corresponding to each of the original oligonucleotide sequences attached to the solid support. Hybridization is optionally reversible and the solid support can be returned to its original state for reuse. A labelled dideoxynucleotide can be incorporated at the end of the oligonucleotide provided that the oligonucleotide is hybridized to genomic DNA as template. The nucleotide sequence at the genomic position adjacent to the region matching the oligonucleotide is known and therefore the particular nucleotide which

2018200459 19 Jan 2018 will be incorporated (A, C, G, T or U) is known. Co-dominant scoring is achieved using paired, i.e. two or parallel, i.e. three, flanking oligonucleotide sequences. The results obtained are recorded as full, empty, failure or null alleles and can be used to distinguish between heterozygous and/or homozygous genotypes. Optional post5 hybridization treatments, including washing and digestion, are provided in order to remove sample DNA not fully hybridized to the solid support-attached oligonucleotide sequences, for example before and after labelling. The presence or absence of hybridization is recorded using a method allowing the recording of the hybridization state, typically on a computer readable medium.

B. Breeding Programs

Stevia is typically an outcrossing species, although self-polination is occasionally observed. Thus, a Stevia plant breeding program typically involves the use of one or more of: recurrent selection mass selection, bulk selection, and intercrossing. These techniques can be used alone or in combination with one or more other techniques in a breeding program. See, Yadav et al., Can, J, Plant Sci. 91: 1-27 (2011). Each identified plant can be crossed to a different plant to produce seed, which is then germinated to form progeny plants. Seed from one or more progeny plants possessing the desired phenotype(s) and desired polymorphism(s) is composited and then randomly mated to form a subsequent progeny generation. The breeding program can repeat these steps for an additional 0 to 5 generations as appropriate in order to achieve the desired stability in the resulting plant population, which retains the polymorphic allele(s). In most breeding programs, analysis for the particular polymorphic allele will be carried out in each generation, although analysis can be carried out in alternate generations if desired. Selfing of progeny plants may be carried out for those stevia lines and populations in which selfing is feasible.

Recurrent selection is a method used in a plant breeding program to improve a population of plants. The method entails individual plants cross pollinating with each other to form progeny. The progeny are grown and the superior progeny selected by any number of selection methods, which include individual plant, half-sib progeny, full-sib progeny and selfed progeny. The selected progeny are self pollinated or cross pollinated with each other to form progeny for another population. This population is

2018200459 19 Jan 2018 planted and again superior plants are selected to self pollinate or cross pollinate with each other. Recurrent selection is a cyclical process and therefore can be repeated as many times as desired. The objective of recurrent selection is to improve the traits of a population. The improved population can then be used as a source of breeding material to obtain new varieties for commercial or breeding use, including the production of a synthetic cultivar. A synthetic cultivar is the resultant progeny formed by the intercrossing of several selected varieties. The number of parental plant varieties, populations, wild accessions, ecotypes, etc., that are used to generate a synthetic can vary from as little as 10 to as much as 500. Typically, about 100 to 300 varieties, populations, etc., are used a parents for the synthetic variety. Seed from the parental seed production plot of a synthetic variety can be sold to the farmer. Alternatively, seed from the parental seed production plot can subsequently undergo one or two generations of multiplication, depending on the amount of seed produced in the parental plot and the demand for seed.

Mass selection is a useful technique when used in conjunction with molecular marker-assisted selection. In mass selection, seeds from individuals are selected based on phenotype or genotype. These selected seeds are then bulked and used to grow the next generation. Bulk selection requires growing a population of plants in a bulk plot, allowing the plants to self-pollinate, harvesting the seed in bulk and then using a sample of the seed harvested in bulk to plant the next generation. Also, instead of self pollination, directed pollination could be used as part of the breeding program.

Thus, in some embodiments, a method of making a Stevia plant line or population involves identifying one or more plants in the line or population in which the presence of a polymorphism at a locus having nucleotide sequence encoding a polypeptide that is at least 90% identical to SEQ ID NOs: 1, 3, 5, or 7 is associated with variation in a trait of interest. The identified plant(s) is then crossed with itself or a different stevia plant to produce seed, and at least one progeny plant grown from the seed is again crossed with itself or a different stevia plant for an additional 0-5 generations to make a line or population that possesses the polymorphism.

In some cases, selection for other useful traits is also carried out, e.g., selection for disease resistance. Selection for such other traits can be carried out before, during

2018200459 19 Jan 2018 or after identification of individual plants that possess the desired polymorphic allele.

Marker-assisted breeding techniques may be used in addition to, or as an alternative to, other sorts of identification techniques.

The invention will be further described in the following examples, which do not limit the scope of the invention described in the claims.

VI. Examples

Example 1 -- Construction of Kaurene Biosynthesis Pathway Genes

A nucleotide sequence encoding a truncated baker’s yeast HMG CoA o reductase was cloned into a yeast high copy episomal plasmid vector such that the coding sequence was operably linked to and under the transcriptional control of a promoter which can be repressed by the amino acid methionine. See, U.S. Patent Nos. 5,460,949 and 5,306,862.

Nucleotide sequences encoding the GGPPS enzymes shown in Table 1 were modified for expression in yeast (see SEQ ID NOs: 18-25) and cloned into an E. coli vector such that the coding sequence was operably linked to and under the transcriptional control of a yeast promoter which can be repressed by the amino acid methionine. The name for each expression cassette-containing plasmid (“entry vector”) is also shown in Table 1. The nucleotide sequences from the source organisms from which the polypeptides were originally identified are set forth in SEQ ID NOs: 26-33. Other entry vectors were constructed using GGPPS enzymes expressed by an unmodified nucleotide sequence from Catharanthus roseus designated EV270, an unmodified nucleotide sequence from Aspergillus nidulans designated C301 and an unmodified nucleotide sequence from Xanthophyllomyces dendrorhous designated C413.

Table 1. GGPPS Clones

Enzyme Source Organism	gi Number	Accession Number	Plasmid Name	Construct Name	Length (nts)	SEQ ID (DNA)	SEQ ID (protein)
Stevia rebaudiana	90289577	ABD92926	pMUS14	MM-1	1086	18	121
Gibberella fujikuroi	3549881	CAA75568	pMUS15	MM-2	1029	19	122

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Mus musculus	47124116	AAH69913	pMUS16	MM-3	903	20	123
Thalassiosira pseudonana	223997332	XP 0022883 39	pMUS17	MM-4	1020	21	124
Streptomyces clavuligerus	254389342	ZP_05004570	pMUS18	MM-5	1068	22	125
Sulfulobus acidocaldarius	506371	BAA43200	pMUS19	MM-6	993	23	126
Synechococcus sp.	86553638	ABC98596	pMUS20	MM-7	894	24	127
Arabidopsis thaliana	15234534	NP 195399	pMUS21	MM-8	1113	25	128

Nucleotide sequences encoding the CDPS enzymes shown in Table 2 were modified for expression in yeast (see SEQ ID NOs: 34-36) and cloned into yeast entry vectors. The nucleotide sequences from the source organisms from which the polypeptides were originally identified are set forth in SEQ ID NOs: 37-39. Other entry vectors were constructed using CDPS enzymes expressed by an unmodified nucleotide sequence from Arabidopsis thaliana designated EV64, an unmodified nucleotide sequence from Zea mays designated EV65 and an unmodified nucleotide sequence from Lycopersicon esculentum designated EV66.

Table 2. CDPS Clones

Enzyme Source Organism	gi Number	Accession Number	Plasmid Name	Construct Name	Length (nts)	SEQ ID: (DNA)	SEQ ID (protein)
Stevia rebaudiana	2642661	AAB87091	pMUS22	MM-9	2364	34	129
Streptomyces clavuligerus	197705855	EDY51667	pMUS23	MM-10	1584	35	130
Bradyrhizobium japonicum	529968	AAC28895.1	pMUS24	MM-11	1551	36	131

Nucleotide sequences encoding the KS enzymes shown in Table 3 were modified for expression in yeast (see SEQ ID NOs: 40 -43) and cloned into yeast entry vectors. The nucleotide sequences from the source organisms from which the polypeptides were originally identified are set forth in SEQ ID NOs:44-47. Other entry vectors were constructed using KS enzymes expressed by an unmodified nucleotide sequence from Arabidopsis thaliana designated EV70, an unmodified nucleotide sequence from Cucurbita maxima designated EV71 and an unmodified nucleotide sequence from Cucumis sativus designated EV72.

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Table 3. KS Clones

Enzyme Source Organism	gi Number	Accession Number	Plasmid Name	Construct Name	Length (nts)	SEQ ID (DNA)	SEQ ID (protein)
Stevia rebaudiana	4959241	AAD34295	pMUS25	MM-12	2355	40	132
Stevia rebaudiana	4959239	AAD34294	pMUS26	MM-13	2355	41	133
Zea mays	162458963	NP 001105097	pMUS27	MM-14	1773	42	134
Populus trichocarpa	224098838	XP002311286	pMUS28	MM-15	2232	43	135

Nucleotide sequences encoding the CDPS-KS fusion enzymes shown in Table 4 were modified for expression in yeast (see SEQ ID NOs: 48 and 49) and cloned into yeast entry vectors. The nucleotide sequences from the source organisms from which the polypeptides were originally identified are set forth in SEQ ID NOs: 50 and 51.

Table 4. CDPS-KS Clones

Enzyme Source Organism	gi Number	Accession Number	Plasmid Name	Construct Name	Lengt h (nts)	SEQ ID (DNA)	SEQ ID (protein)
Phomopsis amygdali	18670430 6	BAG30962	pMUS29	MM-16	2952	48	136
Physcomitrella patens	14632598 6	BAF61135	pMUS30	MM-17	2646	49	137

Nucleotide sequences encoding the KO enzymes shown in Table 5 were modified for expression in yeast (see SEQ ID NOs: 52-55) and cloned into yeast entry vectors. The nucleotide sequences from the source organisms from which the polypeptides were originally identified are set forth in SEQ ID NOs: 56-59.

Table 5. KO Clones

Enzyme Source Organism	gi Number	Accession Number	Plasmid Name	Construct Name	Length (nts)	SEQ ID (DNA)	SEQ ID (protein)
Stevia	76446107	ABA42921	pMUS31	MM-18	1542	52	138

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rebaudiana
Arabidopsis thaliana	3342249	AAC39505	pMUS32	MM-19	1530	53	139
Gibberella fujikoroi	4127832	CAA76703	pMUS33	MM-20	1578	54	140
Trametes versicolor	14278967	BAB59027	pMUS34	MM-21	1500	55	141

Nucleotide sequences encoding the KAH enzymes shown in Table 6 were modified for expression in yeast (see SEQ ID NOs: 60-64) and cloned into yeast entry vectors. The nucleotide sequences from the source organisms from which the polypeptides were originally identified are set forth in SEQ ID NOs: 65-69.

Table 6. KAH Clones

Enzyme Source Organism	gi Number	Accession Number	Plasmid Name	Construct Name	Length (nts)	SEQ ID (DNA)	SEQ ID (protein)
Stevia rebaudiana			pMUS35	MM-22	1578	60	142
Stevia rebaudiana	189418962	ACD93722	pMUS36	MM-23	1431	61	143
Arabidopsis thaliana	15238644	NP_197872	pMUS37	MM-24	1578	62	144
Vitis vinifera	225458454	XP_002282091	pMUS38	MM-25	1590	63	145
Medicago trunculata	84514135	ABC59076	pMUS39	MM-26	1440	64	146

* = Sequence is shown in U.S. Patent Publication No. 2008-0064063.

Nucleotide sequences encoding the CPR enzymes shown in Table 7 were 10 modified for expression in yeast (see SEQ ID NOs: 70-72) and cloned into yeast entry vectors. The nucleotide sequences from the source organisms from which the polypeptides were originally identified are set forth in SEQ ID NOs:73-75.

Table 7. CPR Clones

Enzyme Source Organism	gi Number	Accession Number	Plasmid Name	Construct Name	Length (nts)	SEQ ID (DNA)	SEQ ID (protein)
Stevia rebaudiana	93211213	ABB88839	pMUS40	MM-27	2133	70	147
Arabidopsis thaliana	15233853	NP_194183	pMUS41	MM-28	2079	71	148
Giberella fujikuroi	32562989	CAE09055	pMUS42	MM-29	2142	72	149

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Example 2 — Construction of Steviol Glycoside Pathway Genes

Integration vectors containing nucleotide sequences encoding the UGT85C2 and UGT74G1 enzymes listed in Table 8 were transformed into yeast. Transformants were obtained that contained UGT85C2, or UGT85C2 and UGT74G1, integrated into the genome.

Table 8. UGT Clones

Source Organism	UGT No.	gi Number	Accession Number	Type	Plasmid Name	Length (nucleotides)	SEQ ID
Stevia rebaudiana	UGT85C2	37993660	AY345978.1	Integration vector	pMUSll	1446	4
Stevia rebaudiana	UGT74G1	37993668	AY345982	Integration vector	pMUS12	1383	2
Stevia rebaudiana	UGT76G1	37993652	AY345974	Integration vector	pMUS13	1377	8
Ipomoea purpurea	IP3GGT	62857205	AB192315.1	High copy vector	pMUSlO	1380	77
Beilis perennis	UGT94B1 R25S mutant	56550538 (wild type)	AB 190262.1 (wild type)	High copy vector	pEFl156	1317 (wild type)	79
Arabidopsis thaliana	UGT79B3	28951020	BT005370.1	High copy vector	pEFl153	1362	151

Nucleotide sequences encoding the IP3GGT and UGT94B1 R25S enzymes were modified for expression in yeast (see SEQ ID NOs: 77 and 79) and cloned into yeast entry vectors. Amino acid sequences for IP3GGT and UGT94B1 R25S are set forth in SEQ ID NOs: 76 and 78, respectively. The high copy episomal vector containing a modified IP3GGT nucleotide sequence was designated pEFl 155. The high copy episomal vector containing a modified UGT94B1 R25S nucleotide sequence was designated pEFl 156.

Example 3 — Construction of Yeast Strains

A yeast strain designated EFSC301 was modified by replacing the endogenous ERG9 promoter with the copper inducible CUP1 promoter. Strain EFSC301 is a derivative of EUROSCARF collection yeast strain BY4742. See, the world wide web at uni-frankfurt. de/fb 15/mikro/euroscarf/data/by.html. In standard yeast growth medium, the ERG9 gene is transcribed at very low levels, since the concentration of copper in such medium is low. The decrease in ergosterol production in this strain results in increased amounts of isoprene units available for steviol biosynthesis. The

2018200459 19 Jan 2018 yeast strain was also modified by genomically integrating the Stevia UGT85C2 and

UGT74G1 genes, each under the transcriptional control of the strong constitutive

GPD1 promoter. See Table 8. The strain has one copy of each of the Stevia

UGT85C2 and UGT74G1 genes integrated in the MUS1241 strain genome.

Example 4 — Analysis of Steviol Glycoside Pathway Gene Expression in Yeast

To examine steviol glycoside biosynthesis in yeast, the expression cassettes of the 36 entry vectors of Tables 1-7 and Example 1 were randomly concatenated in ligation reactions to create artificial yeast chromosomes (“eYACs”). The process is shown schematically in FIG 5.

Two different sets of ligations were carried out. Ligation set A included all genes listed in Tables 1-7, except that no bi-functional CDPS-KS genes (Table 4) were included. Ligation set B included all genes listed in Tables 1-7 except that no mono-functional CDPS and KS genes (Tables 2-3) were included.

From 30 to 200 pg of DNA was prepared from each of the cassette-containing entry vectors. The gene expression cassettes were released from each vector by digestion with the restriction enzyme Ascl. The cassettes were then randomly concatenated into eYACs by ligation with T4 ligase in a 3 hour reaction. The success of the concatenation reaction was assessed by the viscosity of the reaction mixture, since concatenated DNA is highly viscous. DNA fragments (“arms”) containing a centromere, two telomeres and the LEU2 and TRP1 selection markers were added to the end of the concatenated expression cassettes, thereby creating functional eYACs.

The eYACs were transformed into spheroplasts of the competent yeast strain MUS1243 by zymolyase digestion of the yeast cell wall, followed by treatment with a

CaCL/PEG buffer, making the spheroplasts permeable to large molecules such as eYACs.

After transformation, the yeast spheroplasts were embedded in a noble agar based solid growth medium, in which regeneration of the cell wall can take place. Colonies appeared from 4-8 days after inoculation. The regeneration medium lacked the amino acids leucine and tryptophan, thus selecting for the presence of doublearmed eYACs in the yeast cells.

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About 3,000 transformants were obtained for each set. Each transformant was re-streaked and tested for yeast strain markers and the genetic presence of both arms of the eYAC, i.e., the LEU2 and TRP1 markers. More than 97% of the transformants had the correct genotype. Each transformant was given a CEY designation number.

Initially, 24 CEYs from each set were grown for 24 hours in 2 ml of Synthetic

Complete medium (SC), without methionine, so as to induce gene expression from the eYACs. After 24 hours, the supernatant from each culture was collected and subjected to LC-MS (Liquid Chromatography-coupled Mass Spectrometry (Triple Quadropole)) analysis for the presence of rubusoside. Since the Stevia UGT74G1 and

UGT85C2 genes are co-expressed in each CEY transformant, the expected end product when steviol is produced is rubusoside (steviol-(13-p-D-glucopyranosyloxy)β-D-glucopyranosyl ester).

None of the CEYs from set B produced detectable levels of rubusoside, whereas 7 of the CEYs from set A did. Strain CEY 19 was the top producer. CEY 19 produced a compound with a mass of 665.2, which could correspond to a sodium adduct of rubusoside. A compound with a mass of 643.2 also was seen, and probably corresponds to protonated rubusoside. MS-MS-based molecular fractionation of the 665.2 mass compound resulted in a break down mass of 503.2, which corresponds to steviol monoside as a sodium adduct. Since the mass, the fractionation pattern, the

HPLC spectrum, and the retention standard of this compound corresponded exactly to that of a rubusoside standard produced in vitro by the glucosylation of steviol using Stevia enzymes 85 C2 and 74G1, the compound produced by CEY was determined to be rubusoside.

Additional screening for rubusoside production

An additional 95 clones from set A and 95 clones from set B were grown in 96 deep-well trays in 1 ml SC medium without methionine. Supernatants from each of these cultures were combined in pools of two clones, analyzed by LC-MS, and the MS signal/noise ratio determined. The MS s/n ratio is an approximate measure of the relative rubusoside content. When a pool of 2 CEYs was found to produce rubusoside, each clone in that pool was analyzed separately. The results showed that no set B CEYs produced rubusoside, while at least 28 CEYs from set A produced detectable levels of rubusoside.

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Identification of genes present in rubusoside producing CEY clones

To correlate the gene content of eYACs to rubusoside production, a PCR protocol was developed in which similar sized fragments (0.5 kb) of all the possible eYAC-borne genes could be amplified. Internal primers of 20-25 nt were placed so that a similar annealing temperature could be used to amplify all genes. Genomic DNA, which includes eYAC DNA, was prepared from 4 CEYs with no rubusoside production, 4 with low rubusoside production and 6 with high to very high rubusoside production. Using equimolar amounts of these 14 DNA preparations, analytical PCR was performed for all 37 genes for these 14 CEYs, as well as positive and negative controls. All genes were amplified except one, apparently due to primer failure.

The genes present in the six high rubusoside-producing CEY strains are shown in Table 9. The genes present in the eight low or no rubusoside-producing CEY strains are shown in Table 10.

Table 9. Genes Present in High Rubusoside-Producing CEY Strains

	HIGH production	VERY high production
Gene	CEY50	CEY 176	CEY 19	CEY 173	CEY191	CEY213
tHMGl	+	+	+	+	-	+
MM-1	-	+	+	+	+	-
MM-2	-	+	+	+	+	-
MM-3	+	+	+	+	+	+
MM-4	+	+	+	-	+	+
MM-5	+	+	+	+	+	+
MM-6	+	+	+	+	+	+
MM-7	-	+	-	+	+	-
MM-8	+	+	+	+	-	+
EV270	+	+	-	+	+	+
C301	+	+	+	+	+	+
C413	+	+	-	+	+	+
MM-9	+	+	+	+	+	+
MM-10	+	-	-	+	+	+
MM-11	+	+	-	+	+	+
EV64	+	+	+	+	+	+
EV65	-	-	+	+	+	+
EV66	+	+	+	+	+	+
MM-12	+	-	-	+	+	+
MM-13	+	+	+	+	+	+
MM-14	+	+	+	+	+	+
MM-15	-	-	-	-	+	-
EV70	-	+	+	+	-	-
EV71	Primers failed
EV72	+	+	+	+	+	+
MM-18	+	+	+	+	+	-
MM-19	+	-	+	-	+	+

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	MM-20	+	+	+	+	+	+
	MM-21	-	-	+	+	-	+
	MM-22	+	+	+	+	+	+
	MM-23	+	-	+	+	-	+
	MM-24	+	+	+	+	+	+
	MM-25	+	+	+	+	+	+
	MM-26	+	+	+	+	+	+
	MM-27	+	+	+	+	+	+
	MM-28	-	-	-	-	-	-
	MM-29	+	+	+	+	+	+
Tab	le 10. Genes Present in CEY Strains Producing Low or No Rubusoside
	NO rubusoside production	LOW production
Gene	CEY 162	CEY 169	CEY171	CEY188	CEY75	CEY 147	CEY214	CEY87
tHMGl	-	-	-	-	-	-	+	+
MM-1	+	+	+	+	-	+	-	-
MM-2	+	-	+	+	+	+	+	+
MM-3	+	+	+	+	+	+	+	+
MM-4	-	-	+	-	-	+	-	+
MM-5	+	+	+	+	+	+	+	+
MM-6	+	+	+	-	+	+	+	+
MM-7	+	-	+	+	+	+	+	+
MM-8	+	+	+	+	+	+	+	+
EV270	+	+	+	+	+	+	+	+
C301	+	+	+	+	+	+	+	+
C413	+	+	+	+	+	+	+	+
MM-9	+	+	+	+	-	+	+	+
MM-10	+	+	+	+	-	+	+	+
MM-11	+	+	+	+	+	+	+	-
EV64	+	+	+	+	-	+	+	+
EV65	+	-	-	-	+	-	+	-
EV66	+	+	+	+	+	+	+	+
MM-12	+	+	+	+	+	+	+	+
MM-13	+	+	+	+	+	+	+	+
MM-14	+	+	+	+	+	+	+	+
MM-15	+	-	+	-	+	+	-	+
EV70	+	+	+	+	+	+	+	+
EV71	Primers failed
EV72	+	+	+	+	+	+	+	+
MM-18	+	+	+	+	+	+	+	+
MM-19	+	+	+	+	+	+	+	+
MM-20	+	+	+	+	+	+	+	+
MM-21	-	+	-	-	-	+	-	+
MM-22	+	+	+	+	+	-	+	+
MM-23	+	-	+	-	+	+	-	+
MM-24	+	-	+	+	+	+	+	+
MM-25	+	-	+	+	+	+	+	+
MM-26	+	+	+	+	+	-	+	+
MM-27	+	+	+	+	+	+	+	+
MM-28	-	-	+	-	-	-	-	+

MM-29

2018200459 19 Jan 2018

Example 5 — Modification of Yeast Culture Conditions

Experiments were carried out with strain CEY213 in order to determine culture conditions conducive to maximum rubusoside production. The starting material was a glycerol freezer stock (-80°C) of CEY213. Frozen cells originally came from an agar plate containing SC yeast medium without tryptophan, leucine and histidine (SC-TLH), and containing 2 mM methionine. Five ml of liquid SC-TLH medium containing 2 mM methionine was inoculated with a loop-full of freeze stock CEY213 yeast cells. eYAC expression in CEY213 is repressed under these conditions. The cells were grown overnight at 30° C with slow shaking (170 rpm) and were designated as “pre-cultures.”

The CEY 213 pre-cultures were used to inoculate 25-50 ml of SC media without methionine, in which the parameters indicated below were varied.

Rubusoside production under each of the growth conditions was measured by centrifuging 500 pi of each culture medium, transferring 250 μΐ of the supernatant to a new tube, adding 250 μΐ methanol, shaking thoroughly and centrifuging for 10 minutes at maximum speed. An aliquot of the supernatant was analyzed for rubusoside production by LC-MS.

Copper Levels

CEY213 precultures were grown in SC medium to which 50 μΜ bathocuproinedisulfonic acid was added. Bathocuproinedisulfonic acid chelates copper in the growth medium. The ERG9 gene in CEY213 has been modified so that expression is controlled by the CUP1 promoter. A decrease in copper levels in the medium will further decrease ERG9 activity and thereby increase the amount of isoprene units available for steviol biosynthesis.

Chelation of copper ions in the growth medium had a detrimental effect on growth of the yeast culture and rubusoside production was decreased proportionally. These results suggested that even without copper chelation, strain CEY213 is at its minimum rate of ergosterol biosynthesis, and no more isoprene units can be diverted from ergosterol biosynthesis towards steviol glycoside production.

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Glucose

Doubling the available glucose from 2 to 4% had a marginal effect on rubusoside production, about a 5-10% increase in rubusoside production.

Limiting available nitrogen

CEY213 pre-cultures were grown under conditions of limited available nitrogen. Limiting nitrogen during growth of yeast in culture is known to increase production of ergosterol. When the concentration of NH4SO4 was decreased from 4g/l to 2, 1 or 0.4 g/1, the growth rate of CEY213 decreased in proportion to the amount of nitrogen. Rubusoside production decreased proportionally with the decrease in growth.

Aeration of cultures

CEY213 was grown in Ehrlenmeyer flasks with or without baffles. The results indicated that there was at best a marginal effect of increased aeration via the use of baffles. If anything, the lack of aeration via the lack of baffles increased production.

Optical Density at Initiation, Fermentation Time and Growth temperature

Cultures were initiated at two different optical densities, OD₆oo=0.1 or OD₆oo=1.0 of pre-cultured CEY213. Fermentation was then carried out for 24, 48, 72 or 144 hours at a temperature of 20, 25 or 30° C.

As shown in FIG 6, the density of the batch culture at fermentation start, the culture temperature and the length of time in fermentation, in combination, had a significant effect on the amount of rubusoside produced by CEY213. Thus, 144 hours growth of a culture with a starting density of OD₆oo=1.0, at 30° C, resulted in the production of no less than 8.5 mgs/liter of rubusoside.

Example 6 -- Large Scale Production of Rubusoside

A series of fermentation experiments with CEY213 were performed using 3 kinds of yeast medium (rich medium and two types of synthetic medium), varying inoculation density, and changing timing of eYAC gene cassette expression.

Batch Fermentation Conditions

Batch fermentation was carried out by centrifuging a CEY213 pre-culture, discarding the supernatant and re-suspending the cells in 6 liters of SC-TLH medium

2018200459 19 Jan 2018 containing ΙΟΟμΜ methionine and 4% glucose. The OD₆oowas adjusted to 1.0 in a

100 ml Ehrlenmeyer flask without baffles and the cells were allowed to grow for 144 hours at 30° C with slow shaking.

Recovery of Rubusoside

After fermentation, the culture was centrifuged and the supernatant was mixed with an equal volume of methanol, shaken thoroughly, and centrifuged to remove precipitated material. The resulting supernatant was purified by flash C18-silica column chromatography with methanol as the eluent, followed by preparative HPLC to obtain one major compound, with one additional minor compound detected.

The purified compound was analyzed by ^JH and ¹³C NMR, and the data are shown in FIG. 7. The compound was confirmed to be rubusoside based on comparison to ³H and ¹³C NMR literature values for rubusoside. Quantitative analysis indicated that CEY213 fermentation produced 12.8 mgs/liter of rubusoside.

Example 7 — IP3GGT Activity

1. Enzymatic Activity oi Ipomoea purpurea 3 GGT glycosyltransferase in vitro

The enzymatic activity οΐ Ipomoea purpurea 3GGT glycosyltransferase (IP3GGT) using steviol as a substrate was determined in vitro. Genes for Stevia rebaudiana UGT85C2 and IP3GGT glycosyltransferase were each expressed in E.

coli and each enzyme was purified.

The enzymatic reaction was performed in two steps. First, 0.5 mM steviol (9.55 mgs total) was incubated with ca. 0.5 pg UGT85C2 enzyme for 16 hours at 30 °C in a reaction buffer (containing 1 mM UDP-glucose, 100 mM Tris-HCl (pH 8.0), 5 mM MgCfr, 1 mM KC1, 0.1 U/ul calf intestine phosphatase). Then ca. 0.5 pg

IP3GGT enzyme was added and the reaction mixture incubated for an additional 20 hours at 30°C.

Analysis of the reaction products indicated about 100% conversion of steviol to steviol-13-O-monoside, 25% of which was further glycosylated into stevio-13-O1,2-bioside. The theoretical steviol-13-O-1,2-bioside yield was about 4.8 mg. The reaction mixture was then subjected to preparative HPFC, which yielded 2.5 mg steviol-13-O-1,2-bioside (52% purification yield). Using FC-MS, the mass of the purified compound had a different retention time than rubusoside and steviol-13-072

2018200459 19 Jan 2018

1,3-bioside. The purified compound was subjected to *H NMR, heteronuclear single quantum coherence (HSQC)-NMR and heteronuclear multiple bond correlation (HMBC)-NMR analysis, which confirmed that the compound was steviol-13-O-1,2bioside. .

2. In vivo expression of IP3GGT in steviol- or steviol monoside-fed yeast

To determine whether the IP3GGT was active in yeast, the 2μ high copy (episomal) plasmid, pMUSlO, containing an unmodified IP3GGT coding sequence operably linked to a strong GPD1 promoter was transformed into the yeast strain

MUS1245. MUS1245 contains a genomically integrated UGT85C2 expression cassette. The resulting yeast strain was grown in SC medium without histidine to select for the continued presence of the IP3GGT expression plasmid, at a starting density of OD₆oo=0.2. Steviol or steviol monoside was added to the medium at 3 mM. After growth for 72 hours at 30°C, culture supernatants were assayed for the presence of steviol and steviol glucosides by HPLC.

LC-MS analysis indicated that no 1,2-glucosylated steviol-13-O-glucoside was detected after feeding with steviol, although steviol-13-O-monoside could be detected. In contrast, low but detectable amounts of the steviol 1,2-bioside were produced by MUS1245 carrying pMUSlO after feeding with steviol-13-O-monoside.

These results show that the native Ipomoea purpurea 3 GGT coding sequence is expressed in yeast at levels sufficient to obtain detectable in vivo conversion of steviol monoside to steviol 1,2-bioside.

Example 8 -- Modification of Yeast Strains

EXG1 andEXG2

S. cerevisiae may contain enzymes that degrade the 1,2 or 1,3 sugar bonds in steviol 1,2- and steviol 1,3-biosides. To test this possibility, yeast strain CEY213 was grown for 3 days at 30° C on media containing 0.1 mM of each of the two biosides. LC-MS analysis of the culture showed the level of 1,2-bioside to be stable, whereas the 1,3-bond in the 1,3-bioside appeared to completely hydrolyse within the limits of detection of the assay.

2018200459 19 Jan 2018

Twenty-five S. cerevisiae mutants, each disrupted in one known or putative glycoside hydrolase gene, were examined for their ability to degrade steviol biosides.

A culture of each yeast mutant was grown as described above on media containing steviol 1,3-bioside and analyzed by LC-MS. The yeast strain carrying a mutation in the EXG1 (exo-l,3-p-glucanase) gene was found to have lost most of the 1,3-bioside hydrolysing activity. The nucleotide sequence of the yeast EXG1 gene is reported in Vazquez de Aldana et al. Gene 97:173-182 (1991). The yeast strain carrying a mutation in the EXG2 gene (another cxo-1,3-p-gliicanasc) showed a small decrease in hydrolysing activity. Correa, et al., Current Genetics 22:283-288 (1992).

o A double mutant yeast strain (exgl exg2) was made. When the double mutant strain was grown on media containing steviol 1,3-bioside, no hydrolysis of the bioside was detected.

Example 9 - Increased Titer of Steviol Biosynthesis

Individual clones of enzymes from each of the different enzyme classes tested in Example 4 (and Table 11) were examined using eYAC technology to identify particular clones that exhibited the greatest production of steviol from isopentenyl pyrophoshate and farnesyl pyrophosphate. The GGPPS, KO and KAH enzymes have been tested on eYACs, individually or in the case of GGPPS enzymes individually or in pools of two (e.g., Synechococcus sp. + S. acidocaldarius GGPPS or Aspergillus niduians GGPPS alone), in a S. cerevisiae strain expressing all remaining enzymatic steps in the steviol pathway. The results indicated that the Synechococcus spp.

GGPPS clone MM-7 (encoded by SEQ ID NO:24) was the most efficient. GGPPS clones from Aspergillus niduians and Sulfulobus acidocaldarius also were quite active. The results also indicated that among the KO and KAH clones, the Stevia KO clone MM-18 (encoded by SEQ ID NO:52) and the A. thaliana KAH clone MM-24 (encoded by SEQ ID NO:62) resulted in the greatest steviol production.

Table 11.

Source Organism	Enzyme	gi Number	Accession Number	Coding Sequence	Coding Sequence Length (nucleotides)
Stevia rebaudiana	GGPPS-1	158104429	ABD92926	MM-1	1086
Gibberella fujikoroi	GGPPS-2	3549881	CAA75568	MM-2	1029

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Mus musculus	GGPPS-3		BC069913.1	MM-3	903
Thalassiosira pseudonana	GGPPS-4	223997332	XP_002288339	MM-4	1020
Sulfulobus acidocaldarius	GGPPS-6	506371	BAA43200	MM-6	993
Synechococcus sp.	GGPPS-7	86553638	ABC98596	MM-7	894
Cantharanthus roseus	GGPPS-9	1063275	X92893	EV270	1074
Aspergillus nidulans	GGPPS-10	29468175	AF479566	C301	1191
Xanthophyllomyces dendrorhous	GGPPS11	63145970	DQ016502	C413	1131
Stevia rebaudiana	CDPS-1	2642661	AAB87091	MM-9	2364
Streptomyces clavuligerus	CDPS-2	197705855	EDY51667	MM-10	1584
Bradyrhizobium japonicum	CDPS-3	529968	AAC28895.1	MM-11	1551
Arabidopsis thaliana	CDPS-4	18412041	NM_116512	EV-64	2409
Zea mays	CDPS-5	50082774	AY562490	EV-65	2484
Lycopersicon esculentum	CDPS-6	6009477	AB015675	EV-66	2403
Stevia rebaudiana	KS-1	4959241	AAD34295	MM-12	2355
Stevia rebaudiana	KS-2	4959239	AAD34294	MM-13	2355
Zea mays	KS-3	162458963	NP 001105097	MM-14	1773
Populus trichocarpa	KS-4	224098838	XP_002311286	MM-15	2232
Arabidopsis thaliana	KS-5	3056724	AF034774	EV-70	2358
Cucurbita maxima	KS-6	1431869	U43904	EV-71	2370
Cucumis sativus	KS-7	21326756	AB045310	EV-72	2358
Stevia rebaudiana	KO-1	76446107	ABA42921	MM-18	1542
Arabidopsis thaliana	KO-2	3342249	AAC39505	MM-19	1530
Gibberella fujikoroi	KO-3	74676162	094142	MM-20	1578
Trametes versicolor	KO-4	14278966	AB057426	MM-21	1500
Stevia rebaudiana	KAH-1	*		MM-22	1578
Stevia rebaudiana	KAH-2	189418962	ACD93722	MM-23	1431
Arabidopsis thaliana	KAH-3	15238644	NM_122399	MM-24	1578

2018200459 19 Jan 2018

Vitis vinifera	KAH4	225458453	XM 002282055	MM-25	1590
Medicago trunculata	KAH5	84514134	DQ335781	MM-26	1440
Stevia rebaudiana	CPR-1	189098311	DQ269454.4	MM-27	2133
Arabidopis thaliana	CPR-2	145343899	NM_118585	MM-28	2079
Gibberella fujikoroi	CPR-3	32562988	AJ576025.1	MM-29	2142
* U.S. Patent	Publication No. 20080064063

S. cerevisiae strain CEY213, described in Example 4, was transformed with high copy plasmids carrying one of the CDPS or KS genes shown in Table 11, operably linked to the strong GPD1 promoter. Preliminary experiments indicated that overexpression of the Stevia rebaudiana CDPS (CDPS-1, encoded by SEQ ID NO:34) in CEY213 gave an increase in rubusoside production relative to CEY213 that lacked the high copy CDPS-1 overexpressing plasmid. The experiments also indicated that the Stevia rebaudiana KO (KO-1, encoded by SEQ ID NO:52) was the o most active KO of the two tested.

To construct a yeast strain with consistently high levels of steviol glycoside production, expression cassettes containing the GGPPS-10 clone, the KO-1 clone (SEQ ID NO:52) and the KAH-3 clone (SEQ ID NO:62) were stably integrated into the genome of the S. cerevisiae strain CEN.PK 111-61A. Expression of these cassettes was driven by the constitutive GPD1 and TPI1 promoters. In addition, expression cassettes containing KS-1 (SEQ ID NO:40), CDPS-1 (SEQ ID NO:34) and UGT74G1 (SEQ ID NO:2) were stably integrated into the genome. The resulting yeast strain, EFSC1751, however, did not produce any steviol-19-O-monoside when grown at laboratory scale under the conditions described in Example 6.

To determine the basis for the lack of steviol glycoside production in

EFSC1751, CDPS-3, CDPS -4, CDPS -5 and CPR-1 genes, alone or in combination, were expressed in strain EFSC1751. CPR-1 is from Stevia rebaudiana and its sequence can be found at Genbank Accession DQ269454.4. The results showed that CPR-1, when expressed with either CDPS-3, CDPS-4 or CDPS-5, resulted in production of steviol-19-O-monoside in EFSC1751. None of these genes alone in the same strain resulted in any production. These results indicate that the genomically integrated copy of CDPS-1, Stevia enzyme, is non-functional in this yeast construct,

2018200459 19 Jan 2018 whereas the Bradyrhizobium, Arabidopsis or Zea CDPS clones were functional in this construct. In addition, the plant-derived KAH and/or KO genes integrated into the chromosome for this construct appear to require an exogenous CPR for activity. The

CPR from Giberella fujikuroi (MM-29) also appears to be able to work with plant5 derived KAH and/or KO polypeptides.

The two leading GGPPS candidates, GGPPS-6 (encoded by SEQ ID NO:23) and GGPPS-7 (encoded by SEQ ID NO:24), were further expressed individually in a S. cerevisiae strain that has a functional steviol glycoside pathway (including UGT74G1) but no GGPPS genes. Transformants then were analyzed for the production of 19-SMG by LC-MS analysis of culture samples that had been boiled in 50% DMSO for 5 minutes and centrifuged at 16000 relative centrifugal force (RCF) for 5 minutes. It was found that many transformants containing the GGPPS-6expressing plasmid did not produce 19-SMG.

Very few transformants were obtained containing GGPPS-7, indicating that

GGPPS-7 (Synechococcus sp.) may be the more active of the two enzymes, and that the activity could be high enough to confer toxicity. For example, a dramatic increase in GGPP production could result in a drain on a downstream pathway such as ergosterol production. To test this hypothesis, a UPC2-1 gene was co-expressed with GGPPS-7, and ergosterol feeding of the cells was attempted to see if this would rescue growth of cells. However, cell growth was not rescued.

Cell toxicity also may be due to an accumulation of GGPP or a metabolite of

GGPP. To test this hypothesis, CDPS-5 was further overexpressed in the GGPPS-7expressing yeast strain to see if the toxicity could be alleviated by increased GGPP usage. CDPS5 over-expression did appear to rescue growth to some extent since transformants with a plasmid overexpressing this enzyme along with the GGPPS-7 gave rise to a few colonies. The number of transformants was still low. Overexpression of CDPS-5 in a similar strain but with GGPPS-10 instead of GGPPS-7 resulted in a doubling of steviol glycoside production, and these results together could suggest that CDPS is a limiting bottleneck in the introduced steviol glycoside biosynthesis pathway.

In summary, based upon production of 19-SMG or rubusoside in test tube cell cultures at 30°C with yeast medium + 2% glucose, for 24-72 hours, the following

2018200459 19 Jan 2018 conclusions were made with the eYAC constructs: KS-1 (Stevia rebaudiana, encoded by SEQ ID NO:40), KO-1 (5. rebaudiana, encoded by SEQ ID NO:52) and KAH-1 (S. rebaudiana) or KAH-3 (Arabidopsis thaliana, encoded by SEQ ID NO:62) appear to be the best combinations for the steviol pathway. GGPPS-7 (Synechococcus sp.) appears to show the highest amount of activity for this step, but if downstream bottlenecks occur overexpression also could lead to toxicity and overall lower levels of steviol glycosides. All combinations of CDPS and CPR gene analogs were tested and it was found that all 3 CPRs in Table 11 were active, and that combinations of CPR-1 (5. rebaudiana, encoded by SEQ ID NO:70) or CPR-3 (Gibberella fujikuroi, encoded by SEQ ID NO:72) with either CDPS-5 (Zea mays) or CDPS-4 (A. thaliana) were particularly useful. CDPS-5 appears to be the optimal CDPS in the pathway. Combinations can be further tested in a reporter strain with reduced flux to sterol pathways.

To investigate the potential for even higher activity of the CDPS from Zea mays (CDPS-5), this gene was expressed from a 2micron multicopy plasmid using the GPD promoter, with and without a plastid signal peptide, to determine if activity is higher in the cytoplasm when targeting sequences are removed. The nucleotide sequence and amino acid sequence of the CDPS-5 from Zea mays and containing the chloroplast signal peptide are set forth in SEQ ID NOs:80 and 81, respectively. The chloroplast signal peptide is encoded by nucleotides 1-150 of SEQ ID NO:80, and corresponds to amino acids 1 to 50 of SEQ ID NO:81. The plasmid was transformed into the stable rubusoside producer strain (EFSC1859) that has GGPPS-10, CDPS-5, KS-1, KO-1, KAH-3, CPR-1 and UGT74G1 (SEQ ID NO:2) integrated into the genome and expressed from the strong constitutive GPD and TPI promoters.

Furthermore, in strain EFSC1859, expression of squalene synthase, which is encoded by ERG9, was downregulated by displacement of the endogenous promoter with the CUP1 inducible promoter. In addition to these genes, strain EFSC1859 also expresses UGT85C2 (SEQ ID NO:3) from a 2micron multicopy vector using a GPD1 promoter. Rubusoside and 19-SMG production were measured by LC-MS to estimate the production level. The removal of the plastid leader sequence did not appear to increase steviol glycoside production as compared to the wild-type sequence.

2018200459 19 Jan 2018

However, this work demonstrates that the leader sequences can be removed without causing a loss of steviol pathway function.

Similarly, plasmids were constructed for CPR-3, KAH-3 and KO-1 without membrane anchoring sequences (i.e., nucleotides 4-63 of SEQ ID NO:72; nucleotides

4-87 of SEQ ID NO:62; and nucleotides 1-117 of SEQ ID NO:52) and were transformed into strain EFSC1859 with the UGT85C2 integrated on the chromosome rather than on a plasmid. It is expected that these enzymes will be functional without the anchoring sequence.

Example 10 - Identification of Steviol-1,3-O-Monoglucoside 1,2Glucosyltransferase Sequences

Stevia EST Analysis

A tBLASTN search of a Stevia (Stevia rebaudiana) leaf EST (Expressed Sequence Tags) database (Brandle et al., Plant Mol, Biol, 50:613-622, 2002) was carried out using complete Ipomoea (Ipomoea purpurea) UGT79 type UGT (IP3GGT), Beilis (Beilisperennis) UGT94B1, Stevia UGT79A2, Stevia UGT76G1 and Stevia UGT9ID 1 amino acid sequences as queries, thus representing UGTs from all Family 1 glycosyltransferase sub-families known to primarily contain diglycosyltransferases. Partial sequences for 9 previously undescribed UGT genes were identified. One of the partial sequences was from the UGT 79 sub-family (“79EV1”), one from the UGT 76 sub-family (“76-EV1”) and two from the UGT 91 subfamily (“91-EV-l” and “91-EV2”), as well as members of the UGT 71, 72, 78, 84 and 88 sub-families. Seven of the partial sequences were isolated using Stevia cDNA or cDNA libraries as the PCR template for isolation. In addition, two Stevia members of the UGT 76 sub-family were isolated, GenBank accession ACT33422.1 which is a member of the 76G1 sub-family (Mohankumar), and GenBank accession ACM47734.1 which is a member of the 76G2 (Yang) sub-family.

Pyroseguencing

Additional UGT clones were identified and isolated by performing pyrosequencing with Stevia cDNA as follows. Stevia mRNA was prepared from

Stevia leaves, using the Ambion® Micro Poly Purist™ mRNA preparation kit. As a quality control, reverse transcribed mRNA was tested for the presence of the Stevia

2018200459 19 Jan 2018

Rebaudioside A pathway UGT genes 85C2, 74G1 and 76G1, by employing analytical

PCR with oligonucleotide primers identical to 21 nucleotides at the 5’- and 3’-termini of each sequence. The amplified full length mRNA was then used for pyrosequencing and contig assembly (MOgene, St. Louis, MO USA). About 3.4 million reads of an average length of 393 nucleotides were performed, and the resulting raw sequences used to obtain 25907 sequence contigs. A database was constructed, containing publicly available amino acid sequences of a total of ca. 1,500 UGTs. About 150 of the sequenced UGTs were fully annotated UGTs from a wide variety of sub-families. The remaining sequenced UGTs were partially annotated homologs of these. A

BLASTX search was performed (CLC Genomics, Muehltal, Germany), using the

25907 Stevia EST contigs as query, to the fabricated UGT database (Genetic code =

1, Low complexity = Yes, Expect value = 10.0, Word size = 3, No of processors = 2, Matrix = BLOSUM62, Gap cost (open) = 11, Gap cost (extension)= 1). The results suggested that sequences for more than 90 previously unknown UGTs from Stevia were present in the pyrosequencing database.

No additional members of the UGT 79 sub-family or the UGT 94 sub-family were identified in the pyrosequencing database. However, the analysis showed new members of the UGT 76 and 91 sub-families. For a few of the genes, full length sequence data was immediately available from the pyrosequencing EST data. A previously constructed Stevia plasmid cDNA library was used to obtain full-length sequences for those members for which partial sequence data was obtained. An oligonucleotide primer identical to each specific, partial UGT sequence was combined with an oligonucleotide primer identical to the library plasmid vector sequence.

These primers were employed in PCR to obtain the full length product, which was subsequently sequenced. Based on the full length sequence, a second PCR was performed using a proof-reading PCR polymerase enzyme for amplification of the full length UGT gene from a Stevia cDNA library as the template for the reaction. Using this strategy, five members of the UGT 76 sub-family, six members of the UGT 91 sub-family, as well as ten members of other UGT sub-families were isolated.

Each of the 7 UGTs identified from the Stevia EST database, the 2 publicly available Stevia UGT 76 sequences, and the 21 UGTs identified from pyrosequencing was cloned into the E. coli expression vectors pET30A+ or pETDuet (making use of

2018200459 19 Jan 2018 the HIS-tag for purification purposes) and expressed in the autolysis-prone E. coli strains XjA and XjB. For a large number of these UGTs, expression of the UGT protein resulted in the formation of inclusion bodies. In order to overcome formation of those inclusion bodies, some of these UGTs were expressed in the low temperature expression strain “Arctic Express” (Agilent Technologies). For those which failed to express in this system, coupled in vitro transcription-translation of PCR products (TNT®T7 Quick for PCR DNA kit, Promega) was attempted, allowing successful expression of the remaining UGTs. Efficiency of the reaction was ensured by labeling with ³⁵S-methionine, separation on SDS-PAGE and phosphorimaging detection of a protein band of the expected size for the UGT protein in question.

UGT polypeptides from each clone, expressed as described above, were tested for 1,2-glycosylation activity, using steviol-13-O-monoglucoside as substrate. In vitro transcribed/translated protein, corresponding to approximately one fifth of the total protein formed in a 25 pL reaction, was used in an in vitro reaction, using 0.5 mM steviol-13-O-monoglucoside (SMG) as substrate, in a reaction buffer (containing 1 mM UDP-glucose, 100 mM Tris-HCl (pH 8.0), 5 mM MgCl₂, 1 mM KC1, 0.1 U/pl calf intestine phosphatase). The reaction mixture was incubated at 30 °C for 20 hours The reaction mixture was then analyzed by LC-MS analysis for the presence of Steviol-1,2-bioside. LC-MS analyses were performed using an Agilent 1100 Series

HPLC system (Agilent Technologies) fitted with a Phenomenex® Synergy Hydro-RP column (250 x 3 mm, 3 pm particles, 80 A pore size) and hyphenated to a TSQ Quantum (ThermoFisher Scientific) triple quadropole mass spectrometer with electrospray ionization. Elution was carried out using a mobile phase (30 °C) containing MeCN (0.01% Formic acid) and H₂O (0.01% Formic acid) by applying a gradient composed of 0.6^0.4 ml/min, 5% MeCN for 4 min; 0.4 ml/min, 5—>40% MeCN for 2 min; 0.4 ml/min, 40—>55% MeCN for 11 min; 0.4^1.0 ml/min,

55—>100% MeCN for 3 min. Steviol biosides were detected using SIM (Single Ion Monitoring) on Mw 665.2 [M+Na⁺], None of the 30 UGT enzymes tested exhibited detectable steviol-13-O-monoglucoside glycosylation activity.

The nucleotide sequences of the six UGT91 members identified by pyrosequencing were compared to the sequence of Stevia UGT91D1 in Genbank Accession No. AY345980. It appeared that the GenBank sequence encoded 12

2018200459 19 Jan 2018 additional amino acids at the N-terminus, relative to the six sequences identified by pyrosequencing. To re-test UGT9 ID 1 family members for activity, UGT91D1 sequences were re-isolated by PCR amplification of Stevia leaf cDNA. The resulting

PCR products were cloned into a plasmid vector and enzymatic activity for each product was measured as described above by: GST-tagged expression in E. coli, coupled in vitro transcription-translation, and/or in vivo expression in yeast. Steviol 1,2-glucosylation activity was detected from one clone by all three methods. This clone was designated UGT91D2e. The amino acid sequence of UGT91D2e is set forth in SEQ ID NO:5. In contrast, no 1,2-glucosylation activity was detected from a clone having the same sequence as described by Accession No. AY345980 (Protein Accession number AAR06918), but lacking the 12 amino acids of the amino terminus.

Example 11 - Analysis of UGT91D2e Sequences

Sequence variants ofUGT91D2e

As evidenced in FIG. 19B, a small number of amino acid modifications exist between the active (91D2e) variants and the closest inactive homologs (91D1). The 91D1 genes cloned by Ma et al., Shi Yan Sheng Wu Xue Bao, 2003 36(2):123-9 (Protein Accession number AAM53963, GI:21435782 ) and Brandle et al., supra (Protein Accession number AAR06918, GI:37993665) did not exhibit the 1,2glycosylating activity required for RebA biosynthesis. To ascertain which amino acids are required for activity, 21 single site-directed mutants were created such that the amino acid in UGT91D2e (SEQ ID NO:5) was changed to the corresponding amino acid in an inactive homolog. See Table 12. In addition, a site-directed mutation was made such that position 364 (S->P) also was changed. The mutants were made using the QuikChange® II Site-Directed Mutagenesis kit according to manufacturer’s protocols (Agilent Technologies, Santa Clara, CA), and the pGEX-4TI vectors were transformed into a XJb Autolysis E. coli strain (ZymoResearch, Orange, CA). A mutant was not made to change residue 162 from a glycine to an aspartic acid.

In order to assess the activity of the mutant enzymes, a substrate-feeding experiment was performed in vitro using protein produced in E. coli. Initially, E. coli cells were grown overnight at 30°C, followed by induction with 3 mM arabinose and

2018200459 19 Jan 2018

0.1 mM IPTG, and further incubation at 20°C. For the in vitro assay, cells were induced overnight at 20° C, lysed by a freeze/thaw cycle, and the crude cell extract used for an enzymatic reaction in which the substrates were 0.5 mM steviol-13-(9glucoside and 0.5 mM rubusoside.

The results are shown in Table 12 for the steviol monoglucoside (SMG) and

Rubusoside (Rub) substrates. A “+” indicates that diglycosylation activity was detected, a indicates activity was not detected, and “NA” indicates the assay was not performed. The noted mutations are based on the numbering of the 91D2e sequence (SEQ ID NOG).

As some of the genes have a tendency to express in inclusion bodies in E. coli, the coding sequences that did not show activity in the E. coli experiments also were produced by coupled in vitro transcription-translation of PCR products (TNT®T7 Quick for PCR DNA kit, Promega) as above in Example 10. Briefly, 2 pL of DNA from the PCR amplification of the five single mutants and the wild type enzyme were incubated for 90 minutes at 30°C with the kit master mix and 1 pL L-[³⁵S]Methionine, in a total of 25 pL reaction. For each sample, a volume of 2 pL final reaction was run on a SDS-PAGE gel. All six proteins showed similar levels of soluble recombinant protein as judged by visual observation of the SDS-PAGE gel. The results for the in vz'Zra-translatcd proteins are shown on the right side of Table 12.

The percentages in this table indicate the approximate amount of conversion of substrate to product based on relative peak areas of substrate and product.

Table 12

Mutation	E. coli protein SMG	E. coli protein Rub	in vitro protein SMG	in vitro protein Rub
Y30->F	+	+	NA	NA
P93~>Q	+	+	NA	NA
S99~>V	+	+	NA	NA
Y122->F	+	+	NA	NA
H->140Y	+	+	NA	NA

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S142->C	+	+	NA	NA
T144^I	-	-	5.9%	0.05%
A148->T	+	+	NA	NA
M152->L	-	-	25.1%	0.85%
G153->A	+	+	NA	NA
A156->S	+	+	NA	NA
L195->M	+	+	NA	NA
V196->E	+	+	NA	NA
K199->E	+	+	NA	NA
L211->M	+	+	NA	NA
L213^F	-	-	29.4%	1.59%
S221^F	+	+	NA	NA
V286->A	+	+	NA	NA
S364->P	-	-	4.1%	0.4%
G384->C	-	-	14.1%	1.28%
K427->N	+	+	NA	NA
E438->A	+	+	NA	NA

The approximate amount of diglycosylation activity as compared to UGT91D2e (SEQ ID NO:5) was found to be: 6.1% for T144S, 26.2% for M152L, 30.7% for L213F, 4.3% for S364P, and 14.7% for G384C using 13-SMG as substrate.

For rubusoside, the approximate amount of diglycosylation activity as compared to UGT91D2e was 1.4%, 23.4%, 43.7%, 10.9% and 35.2% for T144S, M152L, L213F, S364P, and G384C, respectively.

These results indicate that 5 of the 22 amino acid mutations were noticeably deleterious for activity when done in isolation. It is also possible that combinations of the other 17 mutations also could result in inactivity or loss of activity.

By aligning the 91D2e sequences and the variants described above with proteins termed At72Bl, Mt85H2, VvGTl and Mt71Gl (Osmani et al (2009) Phytochemistry 70, 325-347), and analyzing predicted tertiary structures (alpha helices, beta-sheets, and coil regions), regions can be identified where mutations are likely to result in loss of diglycosylation activity. The first three mutations that are 84

2018200459 19 Jan 2018 deleterious are found in the N-terminal domain, in regions that are thought to be loops. The N-terminal domain (amino acid residues 1-240), in particular the predicted loop regions of the N-terminal domain (amino acids 20-26, 39-43, 88-95, 121-124,

142-158, 185-198, and 203-214), are thought to be primarily responsible for binding of the glucose acceptor molecule substrate. The fourth mutation that appears to be deleterious for activity is found in the C-terminal domain, in a region that is believed to be the C5 loop (corresponding to amino acids 381-386). This loop is also thought to be important for glucose acceptor substrate specificity. Nineteen of the twenty-two mutations that separate the inactive versus the active rubusoside diglycosylase o enzymes are located within five amino acids of the predicted acceptor substrate binding regions of 91D2e. Therefore it is likely that the published 91D1 enzymes catalyze a glycosyl transferase reaction between UDP-glucose and an alternative acceptor substrate.

Example 12 -- Production of Rebaudioside Ain Yeast

Production of Rebaudioside A in Steviol-Fed Yeast

The yeast strain EF SC 15 80, which contains a genomically integrated

UGT74G1 expression cassette, was transformed with three different 2μ high copy (episomal) plasmids for co-expression of Stevia UGTs 91D2e (SEQ ID NO:5), 85C2 (SEQ ID NOG), and 76G1 (SEQ ID NO:7). The three plasmids, designated pMUS44, pMUS7 and pMUS9, contain coding sequences for UGT91D2e, UGT85C2 and UGT76G1, respectively, operably linked to the strong GPD1 promoter. The resulting yeast strain was grown in SC medium without uracil, histidine, and leucine to select for the continued presence of the pMUS44, pMUS7 and pMUS9 expression plasmids.

Steviol was added to the medium to a final concentration of 250μΜ, and the strain was cultured at 30°C. At 18 hours and 72 hours of culture, aliquots of the supernatants and cell pellets were analyzed for the presence of Rebaudioside A by LC-MS. LC-MS analyses were performed using an Agilent 1100 Series HPLC system (Agilent Technologies, Wilmington, DE, USA) fitted with a Phenomenex®

Synergy Hydro-RP column (250 x 3 mm, 3 pm particles, 80 A pore size) and hyphenated to a TSQ Quantum (ThermoFisher Scientific) triple quadropole mass spectrometer with electrospray ionization. Elution was carried out using a mobile

2018200459 19 Jan 2018 phase (30 °C) containing MeCN (0.01% Formic acid) and H2O (0.01% Formic acid) by applying a gradient composed of 0.6^0.4 ml/min, 5% MeCN for 4 min; 0.4 ml/min, 5—>40% MeCN for 2 min; 0.4 ml/min, 40—>55% MeCN for 11 min; 0.4—>1.0 ml/min, 55—>100% MeCN for 3 min. Steviol biosides were detected using SIM (Single Ion Monitoring).

LC-MS results showed that detectable amounts of Rebaudioside A were found in the supernatant at 18 and 72 hours of culture when strain EF SC 15 80 containing pMUS44, pMUS7 and pMUS9 was grown in the presence of steviol. The product coeluted with a Rebaudioside A standard and the expected mass was confirmed as the [M+Na]⁺ = 989. By comparing the absorbance of the product to the absorbance of a

10μΜ Rebaudioside A standard, the accumulation in the supernatant of the cell culture was estimated to be more than 6mg/L at 18 hours, and more than 15 mg/L at 72 hours.

Production of Rebaudioside A and Rebaudioside D in Glucose-Fed Yeast

Yeast strain CEY213, described in Example 4, contains steviol biosynthetic pathway genes expressed from eYACs as well as genomically integrated UGT74G1 and UGT85C2 expression cassettes. Strain CEY213 produces rubusoside, as described in Example 6.

Strain CEY213 was transformed with a 2μ high copy (episomal) dual expression plasmid, pMUS47, for simultaneous expression of UGT91D2e (SEQ ID NO:5) and UGT76G1 (SEQ ID NO:7). The pMUS47 plasmid contains two expression cassettes, one having the coding sequence of UGT91D2e and the other having the coding sequence of UGT76G1. Both coding sequences are operably linked to the strong constitutive GPD1 promoter. The resulting yeast strain was precultured overnight at 30°C in SC medium without histidine, leucine and tryptophan in order to maintain selection for the presence of eYACs, without uracil in order to maintain selection for the presence pMUS47, and finally with methionine (2mM) in order to suppress promoters present on the eYACs. The next day, the cells were washed and transferred to an identical medium, but without methionine, for induction of the eYAC promoters. Samples were collected after 24 hours and 99 hours of

2018200459 19 Jan 2018 incubation, and supernatants and cell pellets analyzed for the presence of

Rebaudioside A and Rebaudioside D, using LC-MS as described above.

The results showed that detectable amounts of Rebaudioside A were found in the supernatants at both 24 and 99 hours. The product co-eluted with a Rebaudioside

A standard and the expected mass was confirmed as the [M+Na]⁺ = 989. By comparing the absorbance of the product to a ΙΟμΜ Rebaudioside A standard, the accumulation of Rebaudioside A in the supernatant was estimated to be more than 3mg/L at 24 hours and more than 6 mg/L at 99 hours. See FIG. 9. The results also indicated that small amounts of stevioside and rubusoside were present in the yeast cell pellet and that detectable amounts of stevioside and rubusoside were present in the culture supernatant. See FIG. 9.

The results also showed that small but detectable amounts of Rebaudioside D were produced, suggesting that UGT91D2e is capable of conjugating an additional glucose to the 19-0 glucose of either stevioside producing Rebaudioside E or directly to the 19-0 glucose of Rebaudioside A. These results also suggest that UGT76G1 may be capable of accepting Rebaudioside E as a substrate to produce Rebaudioside

D. See FIG. 2C.

Example 13 -- Production of Rebaudioside A with codon optimized sequences for

UGT sequences

Optimal coding sequences for UGT 91d2e, 74G1, 76G1, and 85C2 were designed and synthesized for yeast expression using two methodologies, supplied by GeneArt (Regensburg, Germany) (SEQ ID NOs: 6, 2, 8, and 4, respectively) or DNA 2.0 (Menlo Park, CA) (SEQ ID NOs: 84, 83, 85, and 82, respectively). The amino acid sequences of UGT 91d2e, 74G1, 76G1, and 85C2 (SEQ ID NOs: 5, 1, 7, and 3, respectively) were not changed.

High copy number plasmids containing expression cassettes with all four optimized UGTs were constructed and expressed, and their activity compared to expression products of similar constructs containing wild-type sequences. The plasmids were transformed into the universal Watchmaker strain, EFSC301 (described in Example 3). UGTs were inserted in high copy (2μ) vectors and expressed from a strong constitutive promoter (GPDl) (vectors P423-GPD, P42487

2018200459 19 Jan 2018

GPD, P425-GPD, and P426-GPD). After overnight growth and re -inoculation in fresh media at an OD₆oo of 0.25, the culture medium (SC -leu-trp-ura-his) was supplemented with 25 μΜ steviol (final concentration), and production of Rubusoside (Rub), 19-SMG (19SMG) and RebA (RebA) was measured in the media after 24h.

The experiment was repeated, in part due to the fact that 19-SMG was undetectable in one of the first samples.

The results from the two separate studies, shown in Table 13 below, indicate that all eight of the codon-optimized UGTs were active. However, enzyme expression for at least one of the codon-optimized UGTs in each strategy was reduced by the new codon optimization algorithm used to make the constructs. It appears that in the GeneArt modified constructs (SEQ ID NOs: 6, 2, 8, and 4), a bottleneck was potentially created between rubusoside and RebA. It is expected that individual enzyme activity assays and expression analyses of these coding sequences expressed in the yeast strains will allow for the optimal combination of UGT genes in the pathway.

Table 13

	RebA (μΜ)	19SMG (μΜ)	Rub (μΜ)
Wild-type	3.2	17.2	4.9
1.7	14.0	3.2
DNA2.0	4.4	12.4	4.6
1.7	10.8	3.1
GeneArt	1.2	nd	4.6
0.8	11.1	4.5

nd = below detection limit

Example 14 - Production of Rebaudioside A using UGTs with sequence tags

Fusions of small peptides or protein binding domains with the UGT proteins

85C2, 91D2e, 74G1, and 76G1 can promote interactions between the UGTs (channeling) or aid in targeting/anchoring the UGTs to specific components of the yeast cells.

To assess if scaffolding of the UGTs in the RebA pathway could result in active pathway enzymes, the DNA 2.0 codon-optimized UGTs 85C2 and 74G1 were fused in-frame to a string of 4 high-affinity, short (also known as PMI) peptides that

2018200459 19 Jan 2018 resemble the p53 protein motif. The p53 protein motif interacts with the MDM2 protein in humans (see Li et al., J Mol Biol, 2010, 398(2):200-13). DNA 2.0 codonoptimized UGTs 85C2, 91D2e, 74G1 and 76G1 (SEQ ID NOs: 82, 84, 83, and 85, respectively) were fused in-frame to the first 158 amino acids of the human protein

MDM2 (gene accession number ABT17086). A small GS-rich linker region also was fused just prior to the N-terminal methionine of the UGTs. Unfused, the affinity of PMI/MDM2 binding is in the low nM range representing a high-affinity binding. Yeast cells transformed with the above constructs are expected to produce a UGT scaffold around the 4 X PMI (P53-like) peptide repeat fused N-terminally to the 85C2 protein (designated 85C2_P53) scaffold.

The laboratory yeast strain BY4741, deleted for TRP1, was transformed with expression plasmids p423-426 GPD (Mumberg et al, Gene, 156 (1995), 119-122) expressing Stevia rebaudiana UGTs 74G1,76G1 and 91D2e with N-terminal, inframe fusions ofthe first 158 amino acids of human MDM2 protein, and expressing

Stevia rebaudiana UGT85C2 with an N-terminal in-frame fusion of 4 repeats of the synthetic PMI peptide (4 X TSFAEYWNLLSP, SEQ ID NO:86). See SEQ ID NOs: 88, 90, 92, and 94 for the amino acid sequences ofthe 85C2, 74G1, 91D2e, and 76G1 fusion proteins, respectively; see SEQ ID NOs: 89, 92, 93, and 95 for the nucleotide sequences encoding the fusion proteins. This yeast strain and a control strain (expressing the four UGT’s without any fusions) were grown overnight in synthetic yeast medium selecting for the presence of plasmids and then transferred the next day to a 96 deep-well tray containing synthetic yeast medium to a cell density giving an OD₆oo of 1. A final concentration of ΙΟΟμΜ steviol was added. After 72 hours, samples were taken and analysed by LC-MS, as described in Example 12. As indicated in FIGs. 10A and 10B, the UGTs are active in yeast when expressed with the various fusion tags.

Example 15 -UGT91D2e Activity

Additional sub-family 91 UGTs were cloned using cDNA/library preparations made from 3 Stevia sources of different genetic backgrounds. Oligonucleotide primers identical to UGT91Dl/91D2e were used for PCR amplification ofthe cDNA preparations, and the resulting PCR products of correct size were cloned into

2018200459 19 Jan 2018 appropriate plasmid vectors. Numerous clones from each experiment were sequenced, and the sequencing results showed that UGT91D nucleic acids with slight variations in sequence could be amplified. The twenty UGT91D variants with the greatest differences in sequence relative to UGT91D2e were expressed by in vitro transcription-translation followed by enzymatic testing for steviol-13-0monoglucoside-l,2-glucosylating activity. One of the variants showed weak 1,2bioside glucosylation activity, while the reminder showed no detectable glucosylation activity. It therefore appears that UGT91D2 polypeptides are the primary steviol-13O-monoglucoside-l,2-glucosylating enzymes m Stevia.

Enzymatic activity ofUGT91D2e

UGT91D2e (SEQ ID NO:5), made by coupled in vitro transcriptiontranslation, was tested for the ability to xylosylate and rhamnosylate steviol-13-0monoglucoside in an in vitro enzyme assay, using UDP-xylose or UDP-rhamnose as the sugar donors rather than UDP-glucose.

The xylosylation assay was performed as follows: 3 mM UDP-glucuronic acid was mixed with ca. 1 pg Arabidopsis thaliana-cncodcd UDP-glucuronic acid decarboxylase UXS3 (produced in E. coli and then purified), 100 mM Tris-HCl (pH 8.0), 1 mM DTT, 6 pg BSA, 1 mM MgCfl, and 1% calf intestine phosphatase. The reaction mixture was incubated for 30 minutes at 30 °C, in order for UDP-glucuronic acid to be turned into UDP-xylose. Then 1.5 mM steviol-13-O-monoglucoside substrate and ca. 0.5 pg UGT91D2e enzyme made as described in Example 9 was added to the mixture, which was allowed to incubate at 30 °C for an additional 20 hours.

The rhamnosylation assay was performed in the following way: 3 mM UDP25 glucose was mixed with 0.6 pg of each of the N-terminal and C-terminal parts of

Arabidopsis thaliana-cncodcd RHM2 rhamnose synthetase (produced in E. coli and then purified), 100 mM Tris-HCl (pH 8.0), 1 mM DTT, 1.5 mM NADPH, 1.5 mM NAD+, 6 pg BSA, 1 mM MgC’f, and 1% calf intestine phosphatase. The reaction mixture was incubated for 30 minutes at 30 °C, in order for UDP-glucose to be turned into UDP-rhamnose. Then 1.5 mM steviol-13-O-monoglucoside substrate and ca. 0.5 pg UGT91D2e enzyme was added to the mixture, which was allowed to incubate at 30 °C for an additional 20 hours.

2018200459 19 Jan 2018

The results indicated that UGT91D2e was capable of carrying out xylosylation of the steviol-13-(9-monoglucosidc substrate at about one half to one third the rate observed with UDP-glucose, forming 1,2-xylosylated steviol-13-O-monoside, which is a precursor to Rebaudioside F. UGT91D2e was capable of carrying out rhamnosylation of the steviol-13-O-monoglucoside substrate at about the same rate as the rate observed with UDP-glucose, forming 1,2-rhamnosylated steviol-13-0monoside, which is a precursor for Rebaudioside C (Dulcoside B). These results indicate that synthesis of appropriate precursor molecules and expression of appropriate UGTs in vivo should result in the production of Rebaudioside F and C in vivo. See FIGs. 2B and 2D.

UGT91D2e also was tested for its ability to 1,2-glucosylate substrates other than steviol-13-O-monoglucoside in vitro, i.e., rubusoside, steviol-1,3-bioside and 1,3-stevioside. The results indicated that UGT 91D2e was not active when a 1,3bound glucose was present (e.g., steviol 1,3-bioside and 1,3-stevioside), while UGT

91D2e was active regardless of primary glucosylation at the 19-0 position. These results suggest that steviol 1,3-bioside and 1,3-stevioside are likely not present in the in vivo Stevia pathway for rebA formation. See FIG. 2A and FIG. 3.

Example 16 -UGT91D homologs

Different ecotypes of S. rebaudiana are genetically diverse. Investigation of clones of 9 IDs from different Stevia RNA accessions revealed many amino acid changes between six investigated ecotypes (e.g., at nucleotide 74 (resulting in an amino acid change of G to D), 89 (Y to F), 131 (V to A), 137 (F to S), 278 (P to Q), 295 (S to V or P), 331 (E to Q), 365 (Y to F), 395 (A to V), 418 (H to Y), 425 (S to

G), 431 (T to I), 442 (A to T), 454 (M to L), 458 (G to A), 466 (A to S), 485 (G to D),

583 (L to M), 587 (V to E), 595 (K to E), 614 (D to G), 616 (G to R), 631 (L to M), 637 (L to F), 662 (S to F), 664 (K to E), 671 (Y to C), 857 (V to A), 867 (S to R), 919 (F to L), 989 (V to A), 1000 (R to C), 1090 (S to P), 1150 (G to C), 1232 (L to S),

1281 (K to N), 1313 (E to A), 1354 (Q to R), and 1369 (V to I)), as numbered with respect to the nucleotide sequence of 91D2e set forth in SEQ ID NO:9. Some additional variation from these polymorphisms was noted, which is likely due to sequencing or PCR errors, particularly if the polymorphisms were found only once.

Twenty coding regions were chosen for further analysis. See Table 14 for descriptions of clones that were isolated. The numbering of the amino acids in Table is based on the amino acid sequence of UGT91D2e set forth in SEQ ID NO:5.

2018200459 19 Jan 2018

TABLE 14

Clone	Mutations as compared to UGT91D2e (SEQ ID NO:5)
1	+1 frameshift between residues 119-145 in the nucleotide sequence, G165V, 1367V, L388P
2	27 bp deletion starting at nucleotide 728, K214R
3	D205G, V286A, Y443C
4	L28P, Y30F, P93Q, S99V, E111Q, Il 18V, Y122F, H140Y, S142C, T144I, A148T, M152L, G153A, A156S, G162D, L195M, V196E, K199E, L211M, L213F, S221F, L411S, V425A
5	G206R, Y207C, W343R
6	Q13R, F46S, S99P, D395G
7	Y30F, S364P, G384C, K427N, E438A
8	Y94C, A132V, Y224C, G384C, K427N, E438A, Q455R
9	K222E, T341M, G384C
10	Y94C, A132V, Y224C, K313N, R334C, G384C
11	Y30F, K222E, V286A, G384C, K427N, E438A
12	Y30F, P93Q, S99V, Y122F, H140Y, S142C, T144I, T145N, A148T, M152L, G153A, A156S, G162D, L195M, V196E, K199E, L211M, L213F, S221F, V286A S289R, R334C, G384C, K427N, E438A
13	V44A, I136V, G374D, V457I, N463S
14	KOS, K97R, Q103R, F181S, L411S
15	V244A, F307L
16	H140Y, S142C, T144I, A148T, M152L, G153A, A156S
17	L195M, V196E, K199E, L211M, L213F, S221F, V286A, R334C, G384C, K427N, E438A
18	V169A, R334C, G384C, K427N, E438A
19	G25D, Y30F, P93Q, S99V, Y122F, H140Y, S142C, T144I, A148T, M152L, G153A, A156S, G162D, L195M, V196E, K199E, L211M, L213F,

2018200459 19 Jan 2018

	S221F, V286A, G384C
20	I64T, V323A, V33OA, G384C, K427N, E438A

All of the clones in Table 14 were tested for activity using 13-SMG as a substrate. Clone 5 had weak 1,2-glycosylating activity whereas the remaining nineteen did not appear to have activity under the conditions tested. The sequence of clone 5 is set forth in SEQ ID NO:95 and has the following mutations with respect to wild-type UGT92D2e (SEQ ID NO:5): G206R, Y207C, and W343R.

Example 17 -UGT85C homologs

The genetic diversity of UGT85Cs from six different S. rebaudiana ecotypes 1 o was examined to identify homologs that have the same or enhanced activity in pathways for steviol glycoside production. PCR primers were designed that were specific for UGT85C genes, and PCR reactions were carried out on cDNA (some were done on cDNA libraries, some were done on cDNA preparations). The resulting PCR products were cloned and 96 clones were sequenced. Amino acid polymorphisms were mapped and 16 UGT 85C clones were chosen with varying common polymorphism representation. See Table 15. Additional modifications were also noted for some clones, but could be due to PCR errors or were not common polymorphisms. Polymorphisms are described with respect to the nucleotide and amino acid numbering of the wild-type S. rebaudiana UGT85C nucleotide sequence set forth in Accession No. AY345978.1 (see Table 8).

2018200459 19 Jan 2018

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2018200459 19 Jan 2018

2018200459 19 Jan 2018

The clones were expressed through coupled in vitro transcription-translation of PCR products (TNT®T7 Quick for PCR DNA kit, Promega) and assayed for glycosylation activity on the substrates steviol and steviol-19-O-glucosidc (0.5 mM), as described in previous examples. The UGT85Cs produced from clones 1,4, 16, 17, 19,

20, 21, 26, 29, 30, 31, 37, and 39 were soluble and were able to convert 19-SMG to rubusoside in a 90 min assay. The UGT85C produced from clone 27 was considered insoluble. Although UGT85Cs produced from clones 2 and 33 were considered insoluble, trace amounts of rubusoside were produced despite the protein band not being visible. These experiments were independently performed three times. The experiments showed that the following amino acid mutations did not result in a loss of activity: V13F, F15L, H60D, A65S, E71Q, I87F, K220T, R243W, T270M, T270R, Q289H, L334S, A389V, I394V, P397S, E418V, G440D, and H441N. Additional mutations that were seen in active clones include K9E in clone 37, K10R in clone 26, Q21H in clone 2,

M27V in clone 30, L91P in clone 4, Y298C in clone 31, K350T in clone 37, H368R in clone 1, G420R in clone 19, L431P in clone 4, R444G in clone 16, and M471T in clone 30.

The only common polymorphisms that were not tested were T270A and I336T, which are both fairly conservative substitutions. Clone 17 had the most changes incorporated as compared to UGT85C, 6/480 amino acids. The 17-20 amino acids that ’0 appear to be changeable represent approximately a 4% difference at the amino acid level.

Generally, there is low genetic diversity among the 85Cs and it is likely that all of the 85 C homo logs with the common polymorphisms set forth in Table 15 will be active.

Example 18 -UGT76G homologs

The genetic diversity of UGT76Gs from six different S. rebaudiana ecotypes was examined to identify homologs that have the same or enhanced activity in pathways for steviol glycoside production. PCR primers were designed that were specific for UGT76G, and PCR reactions were carried out on preparations of cDNA (cDNA libraries or cDNA preparations). The resulting PCR fragments were cloned and 96 clones were sequenced. Common amino acid polymorphisms were mapped and sixteen UGT76G 96

2018200459 19 Jan 2018 clones chosen, with varying polymorphism representation, including (amino acid numbering): RIOS, I16L, F22V, M29I, K52S, V74K/E, P80S, L85A, V87S/G, L91P, I92F, I93F, H96Y, G97R, L108V, El 13D, Gl 16E, A123T, Q125A, I126L, Y128H, T130A, L142I, V145M, S147N, N151T, F152I, H153L, H155Y, V156D, Q160L,

E163D, L167F, P169L, K188N, K191Q, C192S/F, S193G/A, F194Y, M196N, K198Q,

K199(I, V, Q), Y200(L, A, G), Y203I, F204L, E205G, N206K, I207M, T208I, V217I/F, E226Q, S228P, L230V, V233I, I234T, E236D, I237F, S253P, P266Q, S273P, R274S, G284T/A, T285S, 287-3 bp deletion, R298H, P326A, L330V, G331A, P341L, L346I, S376L, D377A, G379A, L380F, S438P, and K441N. Generally, there was very high diversity among the 76Gs.

The clones were expressed through in vitro translation and assayed for glycosylation activity using 0.5 mM steviol-13-G-glucoside and 0.5 mM stevioside as substrates, as described in previous examples. Reactions were carried out for 90 min at 30 °C. The native 76G1 activity was found in thee new 76Gs designated 76G_C4,

76G G7 and 76G H12, by formation of 1,3-bioside when steviol-13-G-glucoside was used as substrate. Activity in this case was determined comparatively to the positive control, the functional 76G1. Clones 76G G7 and 76G H12 produced slightly higher levels of Reb A than the control but 76G C4 had slightly less Reb A than the control. The number of changes in these clones represents a difference of about 7% at the amino ’0 acid level, from the control enzyme. SEQ ID NOs: 98, 100, and 102 set forth the amino acid sequence of 76G C4, 76G G7, and 76G H12, respectively. SEQ ID NOs: 97, 99, and 101 set forth the nucleotide sequences encoding 76G C4, 76G G7, and 76G H12, respectively. SEQ ID NOs: 98, 100, and 102 set forth the amino acid sequence of 76G C4, 76G G7, and 76G H12, respectively. SEQ ID NOs: 97, 99, and 101 set forth the nucleotide sequences encoding 76G C4, 76G G7, and 76G H12, respectively.

Table 16 summarizes the amino acid changes of the 76G clones that had activity, as compared to the wildtype enzyme. There are a large number of overlapping polymorphisms in the active clones, thus it is expected that these polymorphisms do not cause a loss of activity for the enzyme. It appears that certain mutations are frequent in inactive clones, such as the P->S mutation at position 80 or the F->V mutation at position 22.

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TABLE 16

Clone	Mutations
76GG7	M29I, V74E, V87G, L91P, Gl 16E, A123T, Q125A, I126L, T130A, V145M, C192S, S193A, F194Y, M196N, K198Q, K199I, Y200L, Y203I, F204L, E205G, N206K, I207M, T208I, P266Q, S273P, R274S, G284T, T285S, 287-3 bp deletion, L330V, G331A, L346I
76GH12	M29I, V74E, V87G, L91P, Gl 16E, A123T, Q125A, I126L, T130A, V145M, C192S, S193A, F194Y, M196N, K198Q, K199I, Y200L, Y203I, F204L, E205G, N206K, I207M, T208I, P266Q, S273P, R274S, G284T, T285S, 287-3 bp deletion
76G_C4	M29I, V74E, V87G, L91P, Gl 16E, A123T, Q125A, I126L, T130A, V145M, C192S, S193A, F194Y, M196N, K198Q, K199I, Y200L, Y203I, F204L, E205G, N206K, I207M, T208I

Example 19 - Expression of truncated yeast HMG-CoA reductase and other HMGCoA reductases

In S. cerevisiae, the mevalonate pathway is heavily regulated, for example, at the level of the enzyme 3-Hydroxy-3 -methylglutaryl-coenzyme A (HMG-CoA) reductase.

Expressing a truncated HMG-CoA reductase (tHMGl, encoding an enzyme stabilized from degradation) is one method in which flux towards PPP production can be increased in yeast. For example, expression of tHMGl in yeast has led to dramatic overproduction of β-carotene. See, Verwaal et al., 2007, Appl, Environ, Microbiol. 73:4342. Interestingly, such yeast did not show a darker orange coloration on solid growth medium as was expected, but rather a stronger yellow color, likely due to even higher overproduction of the intermediate phytoene.

To determine if expression of HMG-CoA reductase could be used to improve flux to the steviol and steviol glycoside pathways, a yeast reporter strain for testing isoprenoid 98

2018200459 19 Jan 2018 flux was prepared by substituting the inherent promoter of the ERG9 gene with a CUP1 promoter. See, U.S. Patent Application No. 61/346853, filed May 20, 2010.

The genes used to produce the yeast strain are shown in Table 17. The genes from the source organisms were codon optimized according to DNA 2.0 Inc™. For the purpose of monitoring the cellular prenyl phosphate availability, a construct was produced which had a high copy number plasmid containing gene expression cassettes (methionine-repressible promoters) with the genes for the three enzymes needed to turn prenyl phosphates into β-carotene (GGPP synthase from Xanthophyllomyces dendrorhous, phytoene synthase and beta carotene synthase from A. dendrorhous, and zeta carotene synthase and delta carotene synthase from Neurospora crassa). See, Verwaal et al., 2007 supra; and U.S. Patent Application No. 61/346853.

Table 17 Sources of HMG CoA Reductases and other Mevalonate Genes

Accession#	Organism	Enzyme	Size (nt)	Gene name	SEQ ID (codon optimized)	SEQ ID (protein)
XM_001467423	Leishmania infantum	Acetyl-CoA Cacetyltransferase	1323	MEV-4	103	104
YML075C	Saccharomyces cerevisiae	Truncated HMG (tHMGl)	1584	tHMGl	105	106
EU263989	Ganoderma lucidum	3-HMG-CoA reductase	3681	MEV-11	107	108
BC153262	Bos taurus	3-HMG-CoA reductase	2667	MEV-12	109	110
AAD47596	Artemisia annua	3-HMG-CoA reductase	1704	MEV-13	111	112
AAB62280	Trypanosoma cruzi	3-HMG-CoA reductase	1308	MEV-14	113	114
CAG41604	Staph aureus	3-HMG-CoA reductase	1281	MEV-15	115	116
DNA2.0 sequence	Archaeoglobus fulgidus	3-HMG-CoA reductase	1311	HMG reductase	117	118
DNA2.0 sequence	Pseudomonas mevalonii	3-HMG-CoA reductase	1287	HMG reductase	119	120

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The yeast tHMGl was expressed in the CEN.PK-based yeast strain that produces β-carotene, resulting in a color change from orange to light yellow. Interestingly, expression of the full length HMGs from Artemisia annua, Trypanosoma cruzi and Staphylococcus aureus, as well as the NADH-dependent HMG’s from Pseudomonas mevalonii and Archeoglobus fulgidus produced a similar result, indicating these genes also improve the flux through the mevalonate pathway in yeast (similar overexpression of Bos taurus HMG had no such effect). Finally, the same color change was seen after overexpression of Leishmania infantum acetyl-CoA C-acetyltransferase (first enzyme of mevalonate pathway, described in Tabe 17) or native S. cerevisiae (CAB1, YDR531W) or

B. subtilis, (acc. No. YP004204141) pantothenate kinases (known to result in increased acetyl-CoA production).

To test if the color change in these experiments were indeed due to higher GGPP availability, the yeast tHMGl, P. mevalonii or S. aureus HMGs, or B. subtilis pantothenate kinase were expressed in a stable 19-SMG producer strain. None of these constructs appeared to produce an increase in 19-SMG or rubusoside production (UGT85C2 co-expressed) under the conditions tested. Mevalonate feeding to the yeast reporter strain also did not result in increased rubusoside production. The rubusoside reporter strain, however, has not been genetically modified to reduce the £7?G7-cncodcd flux towards ergosterol biosynthesis. It is expected that control of flux to ergosterol ’0 production would result in increased steviol glycoside production using the HMG reductase genes and other mevalonate pathway genes found to be beneficial to betacarotene production.

Example 20 - Production of RebC in vivo

The synthesis of a precursor molecule to Rebaudioside C, steviol-13-Oglucopyranosyl-l,2-rhamnoside, was shown in vitro in Example 15. In that example steviol-13-O-monoglucoside was used as a substrate, along with UDP-glucose and the Arabidopsis thaliana RHM2 enzyme (locus tag AT1G53500) and UGT91D2e. To further demonstrate the pathway shown in Fig. 2B, production of Rebaudioside C from steviol was accomplished in vivo.

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A yeast strain capable of producing Rebaudioside C was constructed, and production of rebaudioside C and rebaudioside A was assayed by LC-MS. A modified Saccharomyces cerevisiae strain BY4742 was constructed and designated EYS583-7A. The use of BY4742 has been described by Naesby et al., Microb Cell Fact, 8:45 (2009)

All four UGTs (91D2d, 76G1, 74G1, and 85C2) were constitutively expressed iin plasmids with GPD promoters. This type of strain has been described by Naesby et. al, Microb Cell Fact, 8:45 (2009). UGT85C2 was inserted in plasmid P423 GPD (ATCC#87355), UGT74G1 was cloned into P424 GPD (ATCC#87357) and both UGT91D2e and UGT76G1 were cloned into P425-GPD (ATCC#87359) with 91D2e in the original multiple cloning site (MCS), and 76G1 inserted with an additional GPD promoter and a CYC terminator. The resulting strain was transformed with plasmid P426 GPD (ATCC#87361) containing the RHM2 gene expressed from the GPD promoter. The strain was grown on SC medium lacking histidine, leucine, tryptophan and uracil for 24 hours. The culture was then re-inoculated to an ODqoo of 0.4 in fresh media containing

25 μΜ steviol, and the yeast was allowed to grow for 72 more hours before detecting if

Rebaudioside C was present in the supernatant and the cell pellets. Rebaudioside C was quantified using an authentic Rebaudioside C standard (Chromadex, Irvine CA). A total of 1.27 μΜ ± 0.36 μΜ of RebC was detected in the supernatant. Similarly, 3.17 μΜ ±

1.09 μΜ RebA was detected in the cell pellet One of skill in the art will recognize that ’0 different ratios of of RebC to RebA can be obtained by modulation of the activity of the RHM2 enzyme and/or by usage of UGT91D2e or UGT76Gl-like enzymes with higher activity for the UDP-rhamnose reactions. The alternative UGTs can be mutagenized versions of the wildtype enzymes or unique enzymes that are obtained through discovery initiatives.

One of skill in the art will recognize that a yeast strain capable of production of

Rebaudioside A from glucose, such as strain CEY213 transformed with a plasmid containing UGT91D2e and UGT76G1 in Example 12 would produce Rebaudioside C with the addition of the RHM2 gene either via a vector or integrated into the chromosome.

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Example 21 — Production of Steviol Glycosides using UGTs expressed in Escherichia coli

Activity of UGT enzymes in gram negative bacteria

The wildtype genes for UGTs 91D2e, 74G1, 76G1, and 85C2 were cloned individually into E. coli XjB-autolysis BL21(DE3) cells using the pET30 vector system from Novagen (EMD4 Biosciences, Madison, WI), except for UGT91D2e, which was cloned into a pGEX 4T-1 (GE Healthcare, Uppsala, Sweden) vector. Similar cloning was described in Examples 7 and 10. All vectors use an IPTG-inducible promoter. Plasmid DNA was transformed into chemically competent cells as described by the vendor.

Transformants displaying the desired antibiotic resistance were grown overnight at 30°C in 2 mL cultures using NZCYM- media and antibiotic. For in vivo feedings, 5 cultures were grown: UGT 91d2e, 74G1, 76G1, and 85C2 individually, and a mix of all 4 clones. The following day, the cultures were induced to a final concentration of 0.3 mM IPTG and 3 mM arabinose, and grown 2 days at 20°C in the presence of 50 μΜ steviol (UGT74G1, UGT85C2 and the quadruple mix) or 50μΜ rubusoside (UGT91D2e and

UGT76G1). The temperature was raised to 30°C and the cells were grown for one more day. The cells were then harvested by centrifugation at 4000 rpm for 5 min., and the supernatants were removed for LC-MS analysis. The cells were resuspended in 50% DMSO, lysed at 80°C for 5 min and the lysates were analyzed by LC-MS.

’0 For in vitro assays, transformants displaying the desired antibiotic resistance were grown overnight at 30°C in 2 mL cultures using NZCYM- media and antibiotic. The following day, the cultures were induced to a final concentration of 0.3 mM IPTG and 3 mM arabinose, and grown for 24h at 20°C. The cells were then harvested by centrifugation at 4000 rpm for 5 min and resuspended in 200 pL GT-buffer (RBC

Bioscience) and 3 tablets/lOOml of Complete mini, protease inhibitor (Roche), transferred to Eppendorf tubes, vortexed and frozen at -80°C for 1.5 hour. Cells were thawed on ice, and left at room temperature for 3 miutes. When approximately half-way thawed, 15 μί of 0.14 mg/ml H₂O DNase solution + 30 μί 0.05M MgCfi was added to each tube and the samples were incubated for approximately 5 minutes at room temperature. The cells were

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2018200459 19 Jan 2018 centrifuged at maximum speed for 5 minutes. One-hundred pL of supernatant (lysate) was transferred to fresh micro fuge tubes, and 100 pL of glycerol was added.

Enzyme assays were performed by adding 15.15 pL H₂O, 7.5 pL 4X Buffer (400mM Tris, 20mM MgC12, 4mM KC1), 0.3 pL FastAP™ (lu/ pL) from Fermentas,

0.45 pL of a 100 mM stock of UDP-glucose, 0.6 pL of substrate (steviol or rubusoside) and 6 pL of the crude enzyme preparations described above. UGT74G1, UGT85C2, as well as all four UGTs mixed were incubated with steviol. UGT 76G1 and 85C2 were incubated with rubusoside. The enzyme assays were incubated overnight at 37°C. Following centrifugation at 4000 rpm for 5 minutes, 30 pL samples were transferred to a io fresh 96 well plate and 30 pL of DMSO was added. The samples were then subjected to LC-MS analysis. Similar in vitro experiments were also done using steviol 1,2-bioside (for UGT76G1 and UGT74G1) or Rebaudioside B (for UGT74G1) as substrates.

No activity was detected in the in vivo feedings. Table 18 illustrates the results for the in vitro assays.

Table 18

Tube	UGT Clone(s)	Substrate fed	Product detected
1	74G1	Steviol	19-SMG, low levels of rubusoside
2	85G1	Steviol	13-SMG, low levels of rubusoside
3	76G1	Rubusoside	1,3-stevioside, an unknown tetraglycoside
4	91D2e	Rubusoside	stevioside
5	Mix of 4 crude UGT preparations	Steviol	Rubusoside, 1,3-stevioside, trace RebA (no monosides)
6	76G1	Steviol 1,2-bioside	Rebaudioside B
7	74G1	Steviol 1,2-bioside	Stevioside
8	74G1	Rebaudioside B	Rebaudioside A

These results indicate that the UGT enzymes are all active in E. coli cells. However, the substrates may not be readily imported into the cytoplasm. It is expected

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2018200459 19 Jan 2018 that if the steviol were produced in E. coli from precursor pathways, the production of the various steviol glycoside products would be feasible from glucose. It is unexpected that the 74G1 and 85G1 UGTs, which have slightly overlapping substrate specificities, can produce rubusoside from steviol singly. The mix of the four crude enzyme preparations gave very low levels of the monosides, which indicates that the conversion to di- and triglycosides was efficient. With respect to UGT91D2e, the preparation that was used had lost some of its original activity after long-term storage. It is expected that a fresh preparation of the enzyme would have yielded higher levels of Rebaudioside A.

io Example 22 — Production of Steviol Glycosides in Physcomitrella patens

Feeding experiments in moss cells

The genes for UGT 91d2e, 74G1, 76G1, and 85C2 were cloned into

Physcomitrella patens using the pTHUbi:Gateway vector system described in U.S. Patent Publication No. 20100297722. This vector uses a strong maize Ubiquitin promoter. PCR primers were designed to amplify the coding regions in previous examples (native sequences) with the addition of “C ACC” upstream of the start codon. Plasmid DNA was digested with Swal and used for transformation into protoplasts (generally around 0.5xl0⁶ protoplasts). Transformants displaying the desired resistance were grown 1 day in 10 mL cultures and then fed either steviol, rubusoside, or buffer + DMSO as indicated ’0 by Table 19. One-half mL of buffer containing substrate was added per 10 mL of culture, and final concentrations of 0.1% DMSO, 50 μΜ steviol or rubusoside, and 0.125 mM phosphate buffer were added to the cultures. A positive control was done where the YFP (yellow fluorescent protein) was expressed in the presence of steviol or just buffer and DMSO. Cultures were grown 2 more days prior to separation of cells and freezing in liquid nitrogen until further analysis. In some cases multiple UGT-containing plasmids were transformed into the same protoplast cells, to illustrate conversion of multiple steps within the moss cells.

Table 19

Tube

UGT Gene(s)

Substrate fed

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1	YFP (control)	none
2	YFP	Steviol (50 pM)
3	74G1	none
4	76G1	none
5	85C2	none
6	91D2E	none
7	74G1	Steviol (50 pM)
8	76G1	Steviol (50 pM)
9	85C2	Steviol (50 pM)
10	91D2E	Steviol (50 pM)
11	74G1/85C2	none
12	74G1/85C2	Steviol (50 pM)
13	74G1/85C2/91D2E	none
14	74G1/85C2/91D2E	Steviol (50 pM)
15	76G1	Rubusoside (50 pM)
16	91D2E	Rubusoside (50 pM)
17	76G1/91D2E	none
18	76G1/91D2E	Rubusoside (50 pM)

Expression was positive in the controls (tubes 1 and 2) as measured by fluorescent signal observation. The supernatants from the experiments were analyzed by LC-MS;

200 pL of each supernatant sample was mixed with an equal volume of 50 percent

DMSO. The samples were spun (15,700 relative centrifugal force, 10 minutes) and 100 micro liters of the resulting supernatant was analyzed by LC-MS.

Protoplast pellets were thawed on ice and 10 mM Tris-HCl pH 8 containing 3 tablets/100 ml of Complete Mini Protease Inhibitor (Roche) was added to reach a final volume of 150 pL. The solutions were divided in two: 75 pL was transferred to a new tube and protoplasts were pelleted (15,700 relative centrifugal force, 1 minute). Pellets were washed with 75 pL Milli-Q water before resuspenion in 150 pL DMSO (50 105

2018200459 19 Jan 2018 percent). Samples were then heated (80 degrees Celsius, 10 minutes), vortexed and centrifuged (15,700 relative centrifugal force, 10 minutes). Fifty pL of the resulting supernatant was analyzed by LC-MS.

No steviol glycoside production was detectable in supernatants or pellets. It is unknown if the steviol and rubusoside can be transported into moss cells.

In vitro feeding of pellet extracts

In vitro feeding experiments were conducted with samples 1,3,4, 5, 6, 11, 13 and 17). Glass beads (425-600 microns) were added to the remaining 75 pL of the original resuspensions and protoplasts were mechanically lysed by vortexing 3 times, 2 minutes each time, at 4 degrees Celsius and storage on ice in between vortexing. The samples were spun (15,700 relative centrifugal force, 10 minutes, 4 degrees Celsius) and 6 pL of resulting supernatants was used in in vitro enzyme reactions. For the enzyme reactions FastAP™ phosphatase (Fermentas) was used (0.3 U/reaction) and the UDP15 glucose:substrate ratio was 5. The samples were fed either steviol or rubusoside according to Table 20.

Table 20

Cell extract from tube	UGT Gene(s)	Substrate fed
1	YFP	None
1	YFP	0.5 mM steviol
1	YFP	0.5 mM rubusoside
3	74G1	0.5 mM steviol
4	76G1	0.5 mM rubusoside
5	85C2	0.5 mM steviol
6	91D2E	0.5 mM rubusoside
11	74G1/85C2	0.5 mM steviol
13	74G1/85C2/91D2E	0.5 mM steviol
17	76G1/91D2E	0.5 mM rubusoside

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Reactions were incubated at 30 °C overnight. After incubation, an equal amount of DMSO (100 percent) was added to the samples and mixed, then the sample was spun (15,700 relative centrifugal force, 10 minutes) and 30 pL of the resulting supernatant was analyzed by LC-MS.

LC-MS analysis showed conversion of rubusoside to 1,3-stevioside by UGT76G1. None of the other steviol glycosides were detectable. It is unknown if soluble expression of the UGTs occurred in Physcomitrella. It is expected if one UGT is active in the moss cells, the others would also be active if expression occurred. In addition, the cloning was done in a transient manner. Stable integration of the genes is expected to produce additional clones that are active for UGT activity when tested.

Methods are known to those with skill in the art for increasing soluble expression of recombinant proteins. Alternative promoters, ribosome binding sites, codon usage, coexpression with chaperones, and change in temperature are non-limiting examples of methods for increasing soluble expression of recombinant proteins.

Example 23 — Production of Steviol Glycosides in Aspergillus nidulans

Activity o f UGT enzymes in fungal cells

The native genes for UGT 91D2e, 74G1, 76G1, and 85C2 were cloned into ’0 Aspergillus nidulans using a PCR-fabricated expression cassette and the USER vector system. Cloning methods are described in Hansen et al., Appl, Environ, Microbiol. 77: 3044-3051 (2011). Briefly, a nucleotide sequence encoding each UGT was inserted between the constitutive PgpdA promoter and the TtrpC terminator, in a vector containing additionally two targeting sequences for genomic integration and argB as selection marker. Plasmid DNA was transformed into A. nidulans protoplasts according to Nielsen et al., Fungal Genet, Biol, 43:54-64 (2006) and Johnstone et al., EMBO J, 4:1307-1311 (1985). Transformants displaying the desired resistance were grown for 48 hours in 150 mL cultures using minimal media (1% Glucose; 10 mM NaNO₃; mineral mix).

Cell lysates prepared by disruption of the mycelia with glass beads were used to

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2018200459 19 Jan 2018 determine the activities of the individual UGTs in in vitro. The cell lysates of strains expressing 74G1 and 85C2 were incubated with 0.5 mM steviol and the strains expressing 76G1 and 91D2e were incubated with 0.5 mM steviol-13-O-glucoside for 24 hours, and the supernatants further analyzed using LC/MS. No steviol glycosides were detected.

It is unknown whether soluble expression of the UGT enzymes was achieved as these products are not typically visible on SDS-PAGE. Since Aspergillus and Saccharomyces are both fungi, it is expected that additional experimentation would result in active clones. Methods are known to those with skill in the art for increasing soluble expression of recombinant proteins. Alternative promoters, inducer levels, ribosome binding sites, codon usage, co-expression with chaperones, and change in temperature are non-limiting examples of methods for increasing soluble expression of recombinant proteins.

OTHER EMBODIMENTS

It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the ’0 following claims.

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Claims (33)

1. A recombinant host cell, comprising a recombinant gene encoding a recombinant polypeptide capable of beta 1,2 glycosylation of the C2’ of the 13-O-glucose, 19-0glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside;

wherein the polypeptide is capable of transferring a sugar moiety to the C2’ of a glucose in the steviol glycoside;

wherein the steviol glycoside is steviol-13-O-glucoside, rubusoside, stevioside or rebaudioside A, and wherein the recombinant host cell is capable of producing stevioside, rebaudioside E, rebaudioside D, steviol-1,2-bioside, steviol-1,2-xylobioside, steviol-1,2rhamnobioside, 1,2-stevioxyloside, an isomer thereof, and/or a composition thereof upon transfer of the sugar moiety by the polypeptide.

2. The recombinant host cell of claim 1, wherein the polypeptide capable of beta 1,2 glycosylation ofthe C2’ ofthe 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of the steviol glycoside comprises:

(a) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:5, 10, 76, 78, or 150;

(b) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:5 and having at least one amino acid substitution at residues 1-19, 27-38, 44-87, 96-120, 125-141, 159-184, 199-202, 215-380, or 387-473 of SEQ ID NO:5;

(c) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:5 and having an amino acid substitution at one or more residues selected from the group consisting of residues 30, 93, 99, 122, 140, 142, 144, 148, 152, 153, 156, 195, 196, 199, 206, 207, 211,213, 221,286, 343, 364, 384, 427, and 438 of SEQ ID NO:5;

(d) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:5 and having an arginine at residue 206, a cysteine at residue 207, and an arginine at residue 343 relative to SEQ ID NO:5; or (e) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:5 and having a tyrosine or (14166264_1):GGG

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2018200459 19 Jan 2018 phenylalanine at residue 30, a proline or glutamine at residue 93, a serine or valine at residue 99, a tyrosine or phenylalanine at residue 122, a histidine or tyrosine at residue 140, a serine or cysteine at residue 142, an alanine or threonine at residue 148, a methionine at residue 152, an alanine at residue 153, an alanine or serine at residue 156, a glycine at residue 162, a leucine or methionine at residue 195, a glutamic acid at residue 196, a lysine or glutamic acid at residue 199, a leucine or methionine at residue 211, a leucine at residue 213, a serine or phenylalanine at residue 221, a valine or isoleucine at residue 253, a valine or alanine at residue 286, an asparagine or lysine at residue 427, or an alanine at residue 438 and an alanine or threonine at residue 462 relative to SEQ ID NO:5.

3. The recombinant host cell of claim 1, wherein the sugar moiety comprises glucose, rhamnose, and/or xylose.

4. The recombinant host cell of claim 1, wherein the recombinant host cell further comprises one or more of:

(a) a gene encoding a polypeptide capable of glycosylating steviol or the steviol glycoside at its C-13 hydroxyl group;

(b) a gene encoding a polypeptide capable of beta 1,3 glycosylation of the C3’ of the 13-O-glucose of the steviol glycoside; and/or (c) a gene encoding a polypeptide capable of glycosylating steviol or the steviol glycoside at its C-19 carboxyl group.

5. The recombinant host cell of claim 4, wherein:

(a) the polypeptide capable of glycosylating steviol or the steviol glycoside at its C-13 hydroxyl group comprises:

(i) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:3; or (II) a polypeptide having one or more amino acid substitutions at residues 9, 10, 13, 15, 21, 27, 60, 65, 71, 87, 91, 220, 243, 270, 289, 298, 334, 336, 350, 368, 389, 394, 397, 418, 420, 440, 441,444, and 471 of SEQ ID NO:3;

(14166264_1):GGG

111

2018200459 19 Jan 2018 (b) the polypeptide capable of beta 1,3 glycosylation of the C3’ of the 13-Oglucose of the steviol glycoside comprises:

(i) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:7; or (ii) a polypeptide having one or more amino acid substitutions at residues 29, 74, 87, 91, 116, 123, 125, 126, 130, 145, 192, 193, 194, 196, 198, 199, 200, 203, 204, 205, 206, 207, 208, 266, 273, 274, 284, 285, 291, 330, 331, and 346 of SEQ ID NO:7;

and/or (c) the polypeptide capable of glycosylating steviol or the steviol glycoside at its C-19 carboxyl group comprises a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:1.

6. The recombinant host cell of claim 1, wherein the host further comprises:

(a) at least one genetic modification in exo-1,3-p-glucanase (EXG1) and/or exo-1,3-p-glucanase (EXG2), wherein the at least one genetic modification reduces EXG1 and/or EXG2 activity in the host; or (b) at least one genetic modification in squalene synthase (ERG9), wherein the at least one genetic modification reduces ERG9 activity in the host.

7. The recombinant host cell of claim 1, wherein one or more of the genes do not comprise:

(a) a leader sequence;

(b) a signal peptide; and/or (c) a membrane anchoring sequence.

8. The recombinant host cell of claim 1, wherein one or more of the genes:

(a) further comprise a nucleotide sequence coding for a fusion tag; or (b) are expressed as fusion proteins.

9. The recombinant host cell of claim 1, wherein the recombinant host cell comprises a permeabilized recombinant host cell which has been transformed with a gene encoding (14166264_1):GGG

112

2018200459 19 Jan 2018 a polypeptide capable of beta 1,2 glycosylation of the C2’ of the 13-O-glucose, 19-0glucose, or both 13-O-glucose and 19-O-glucose of the steviol glycoside.

10. The recombinant host cell of claim 1, wherein the recombinant host cell comprises a plant cell, a mammalian cell, an insect cell, a fungal cell from Aspergillus genus or a yeast cell from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Yarrowia lipolytica, Candida glabrata, Ashbya gossypii, Cyberlindnera jadinii, Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, Candida boidinii, Arxula adeninivorans, Xanthophyllomyces dendrorhous, or Candida albicans species, an algal cell or a bacterial cell from Escherichia coli species or Bacillus genus.

11. The recombinant host cell of claim 1, wherein the recombinant host cell is a Yarrowia lipolytica cell.

12. A method of producing steviol, one or more steviol glycosides or a steviol glycoside composition in a cell culture, comprising growing the recombinant host cell of claim 1 under conditions in which one or more of the genes are expressed;

wherein steviol, the steviol glycoside, or the steviol glycoside composition is produced by the recombinant host cell;

wherein the one or more steviol glycosides is, or the steviol glycoside composition comprises, stevioside, rebaudioside E, rebaudioside D, steviol-1,2-bioside, steviol-1,2-xylobioside, steviol-1,2-rhamnobioside, 1,2-stevioxyloside, and/or an isomer thereof.

13. The method of claim 12, wherein growing can include inducing expression of one or more of the genes or constitutively expressing one or more of the genes.

14. The method of claim 12, wherein the recombinant host cell is grown in a fermentor at a temperature for a period of time, wherein the temperature and period of time facilitate the production of the steviol glycoside composition.

15. The method of claim 12, wherein steviol, the one or more steviol glycosides or the steviol glycoside composition is produced in a permeabilized recombinant host cell which has been transformed with a gene encoding a polypeptide capable of beta 1,2 glycosylation (14166264_1):GGG

113

2018200459 19 Jan 2018 of the C2’ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of the steviol glycoside.

16. The method of claim 15, wherein the steviol glycoside is rebaudioside D, wherein rebaudioside D is produced upon transfer of a glucose moiety to rebaudioside A.

17. The method of claim 15, wherein the permeabilized recombinant host cell comprises Escherichia coli.

18. The method of claim 11, further comprising isolating the Rebaudioside D, alone or together with at least one other steviol glycoside from the cell culture.

19. The method of claim 18, wherein the isolating step comprises separating a liquid phase of the cell culture from a solid phase of the cell culture to obtain a supernatant comprising Rebaudioside D, alone or together with at least one other steviol glycoside, and:

(a) contacting the supernatant with one or more adsorbent resins in order to obtain at least a portion of Rebaudioside D, alone or together with at least one other steviol glycoside; or (b) contacting the supernatant with one or more ion exchange or reversedphase chromatography columns in order to obtain at least a portion of Rebaudioside D, alone or together with at least one other steviol glycoside; or (c) crystallizing or extracting Rebaudioside D, alone or together with at least one other steviol glycoside;

thereby isolating Rebaudioside D, alone or together with at least one other steviol glycoside.

20. The method of claim 11, further comprising recovering Rebaudioside D, alone or together with at least one other steviol glycoside or the steviol glycoside composition from the cell culture;

wherein the recovered steviol glycoside composition is enriched for Rebaudioside D relative to a steviol glycoside composition from a Stevia plant and has a (14166264_1):GGG

114

2018200459 19 Jan 2018 reduced level of Stevia plant-derived components relative to a steviol glycoside composition obtained from a plant-derived Stevia extract.

21. The method of claim 11, wherein the cell culture comprises:

(a) glucose, fructose, sucrose, xylose, rhamnose, uridine diphosphate (UDP)glucose, UDP-rhamnose, UDP-xylose, and/or N-acetyl-glucosamine; and/or (b) supplemental nutrients comprising trace metals, vitamins, salts, yeast nitrogen base (YNB) and/or amino acids.

22. The method of claim 11, wherein the recombinant host cell comprises a plant cell, a mammalian cell, an insect cell, a fungal cell from Aspergillus genus or a yeast cell from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Yarrowia lipolytica, Candida glabrata, Ashbya gossypii, Cyberlindnera jadinii, Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, Candida boidinii, Arxula adeninivorans, Xanthophyllomyces dendrorhous, or Candida albicans species, an algal cell or a bacterial cell from Escherichia coli species or Bacillus genus.

23. The method of claim 11, wherein the recombinant host cell is a Yarrowia lipolytica cell.

24. An in vitro method for producing one or more steviol glycosides or a steviol glycoside composition, comprising adding one or more of:

(a) a polypeptide capable of beta 1,2 glycosylation of the C2’ of the 13-Oglucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside;

(b) a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-13 hydroxyl group;

(c) a polypeptide capable of beta 1,3 glycosylation of the C3’ of the 13-Oglucose of a steviol glycoside; and/or (d) a polypeptide capable of glycosylating steviol or a steviol glycoside at its C-19 carboxyl group;

and steviol, steviol-13-O-glucoside, rubusoside, stevioside or rebaudioside A to a reaction mixture; and wherein at least one of the polypeptides is a recombinant polypeptide;

(14166264_1):GGG

115

2018200459 19 Jan 2018 producing the one or more steviol glycosides or the steviol glycoside composition;

wherein the steviol glycoside comprises steviol-13-O-glucoside, rubusoside, stevioside or rebaudioside A; and wherein the steviol glycoside composition comprises stevioside, rebaudioside E, rebaudioside D, steviol-1,2-bioside, steviol-1,2-xylobioside, steviol-1,2-rhamnobioside, and/or 1,2-stevioxyloside.

25. The method of claim 24, wherein:

(a) the polypeptide capable of beta 1,2 glycosylation of the C2’ of the 13-0glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of the steviol glycoside comprises:

(i) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:5 or 10;

(ii) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:76, 78, or 150;

(iii) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:5 and having at least one amino acid substitution at residues 1-19, 2738, 44-87, 96-120, 125-141, 159-184, 199-202, 215-380, or 387-473 of SEQ ID NO:5;

(iv) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:5 and having an amino acid substitution at one or more residues selected from the group consisting of residues 30, 93, 99, 122, 140, 142, 144, 148, 152, 153, 156, 195, 196, 199, 206, 207, 211, 213, 221,286, 343, 364, 384, 427, and 438 of SEQ ID NO:5;

(v) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:5 and having an arginine at residue 206, a cysteine at residue 207, and an arginine at residue 343 relative to SEQ ID NO:5; or (14166264_1):GGG

116

2018200459 19 Jan 2018 (vi) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:5 and having a tyrosine or phenylalanine at residue 30, a proline or glutamine at residue 93, a serine or valine at residue 99, a tyrosine or phenylalanine at residue 122, a histidine or tyrosine at residue 140, a serine or cysteine at residue 142, an alanine or threonine at residue 148, a methionine at residue 152, an alanine at residue 153, an alanine or serine at residue 156, a glycine at residue 162, a leucine or methionine at residue 195, a glutamic acid at residue 196, a lysine or glutamic acid at residue 199, a leucine or methionine at residue 211, a leucine at residue 213, a serine or phenylalanine at residue 221, a valine or isoleucine at residue 253, a valine or alanine at residue 286, an asparagine or lysine at residue 427, or an alanine at residue 438 and an alanine or threonine at residue 462 relative to SEQ ID NO:5;

(b) the polypeptide capable of glycosylating steviol or the steviol glycoside at its C-13 hydroxyl group comprises:

(i) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:3; or (II) a polypeptide having one or more amino acid substitutions at residues 9, 10, 13, 15, 21, 27, 60, 65, 71, 87, 91, 220, 243, 270, 289, 298, 334, 336, 350, 368, 389, 394, 397, 418, 420, 440, 441,444, and 471 of SEQ ID NO:3;

(c) the polypeptide capable of beta 1,3 glycosylation of the C3’ of the 13-Oglucose of the steviol glycoside comprises:

(i) a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:7; or (ii) a polypeptide having one or more amino acid substitutions at residues 29, 74, 87, 91, 116, 123, 125, 126, 130, 145, 192, 193, 194, 196, 198, 199, 200, 203, 204, 205, 206, 207, 208, 266, 273, 274, 284, 285, 291,330, 331, and 346 of SEQ ID NO:7;

(14166264_1):GGG

117

2018200459 19 Jan 2018 and/or (d) the polypeptide capable of glycosylating steviol or the steviol glycoside at its C-19 carboxyl group comprises a polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:1.

26. The method of claim 24, wherein the reaction mixture comprises:

(a) the polypeptide capable of beta 1,2 glycosylation of the C2’ of the 13-0glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of the precursor steviol glycoside;

(b) the polypeptide capable of glycosylating steviol or the precursor steviol glycoside at its C-13 hydroxyl group;

(c) the polypeptide capable of beta 1,3 glycosylation of the C3' of the 13-Oglucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of the precursor steviol glycoside;

(d) the polypeptide capable of glycosylating steviol or the precursor steviol glycoside at its C-19 carboxyl group;

(e) glucose, fructose, sucrose, xylose, rhamnose, uridine diphosphate (UDP)glucose, UDP-rhamnose, UDP-xylose, and/or N-acetyl-glucosamine; and/or (f) reaction buffer and/or salts.

27. One or more steviol glycosides or a steviol glycoside composition produced by the method of claim 11.

28. A sweetener composition, comprising the one or more steviol glycosides or the steviol glycoside composition of claim 27.

29. A food product, comprising the sweetener composition of claim 28.

30. A beverage or a beverage concentrate, comprising the sweetener composition of claim 28.

31. A cell culture, comprising the recombinant host cell of claim 1, the cell culture further comprising:

(14166264_1):GGG

118

2018200459 19 Jan 2018 (a) stevioside, rebaudioside E, rebaudioside D, steviol-1,2-bioside, steviol1,2-xylobioside, steviol-1,2-rhamnobioside, 1,2-stevioxyloside, an isomer thereof, and/or the composition thereof produced by the recombinant host cell;

(b) glucose, fructose, sucrose, xylose, rhamnose, uridine diphosphate (UDP)glucose, UDP-rhamnose, UDP-xylose, and/or N-acetyl-glucosamine; and (c) supplemental nutrients comprising trace metals, vitamins, salts, YNB, and/or amino acids;

wherein one or more of stevioside, rebaudioside E, rebaudioside D, steviol-1,2bioside, steviol-1,2-xylobioside, steviol-1,2-rhamnobioside, 1,2-stevioxyloside, an isomer thereof alone, and/or in the composition thereof is present at a concentration of at least 1 mg/liter of the cell culture;

wherein the cell culture is enriched for stevioside, rebaudioside E, rebaudioside D, steviol-1,2-bioside, steviol-1,2-xylobioside, steviol-1,2-rhamnobioside, 1,2stevioxyloside, an isomer thereof, and/or the composition thereof relative to a steviol glycoside composition from a Stevia plant and has a reduced level of Stevia plantderived components relative to a steviol glycoside composition obtained from a plantderived Stevia extract.

32. A cell lysate from the recombinant host cell of claim 1 grown in the cell culture, comprising:

(a) stevioside, rebaudioside E, rebaudioside D, steviol-1,2-bioside, steviol1,2-xylobioside, steviol-1,2-rhamnobioside, 1,2-stevioxyloside, an isomer thereof, and/or the composition thereof produced by the recombinant host cell;

(b) glucose, fructose, sucrose, xylose, rhamnose, uridine diphosphate (UDP)glucose, UDP-rhamnose, UDP-xylose, and/or N-acetyl-glucosamine; and (c) supplemental nutrients comprising trace metals, vitamins, salts, YNB, and/or amino acids;

wherein one or more of stevioside, rebaudioside E, rebaudioside D, steviol-1,2bioside, steviol-1,2-xylobioside, steviol-1,2-rhamnobioside, 1,2-stevioxyloside, an isomer thereof alone, and/or in the composition thereof produced by the recombinant host cell is present at a concentration of at least 1 mg/liter of the cell culture.

(14166264_1):GGG

119

2018200459 19 Jan 2018

33. A reaction mixture, comprising:

(a) one or more of stevioside, rebaudioside E, rebaudioside D, steviol-1,2bioside, steviol-1,2-xylobioside, steviol-1,2-rhamnobioside, 1,2stevioxyloside, an isomer thereof, and/or the steviol glycoside composition thereof produced in the reaction mixture, (b) a polypeptide capable of beta 1,2 glycosylation of the C2’ of the 13-0glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside;

(c) glucose, fructose, sucrose, xylose, rhamnose, uridine diphosphate (UDP)glucose, UDP-rhamnose, UDP-xylose, and/or N-acetyl-glucosamine; and (d) reaction buffer and/or salts.

Evolva, Inc.

Patent Attorneys for the Applicant/Nominated Person

SPRUSON & FERGUSON (14166264_1):GGG

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Sieviol-IS-O-Giucoside Rubusoside

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FIGUR E 5 cDN A cassettes liberated eti masse from Entry libraries Concatenated by ligation $ Chromosome arms are added ® Optima! size sYACs selected by PFGE ¢100-300 kb) e These wili contain from 50»150 expression cassettes eYACs

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2018200459 19 Jan 2018

FIGURE 6

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2018200459 19 Jan 2018

Literature Analyte No m 13C 1H HSQC δ [ppm] M J[Hz] δ [ppm] M J[Hz] 1 40.8 40,7 2 19.4 19.4 3 38.3 38,0 4 44.0 - 5 57.3 57.1 6 22.1 22.0 7 41.1 41.5 8 42.4 - 9 53.9 53.7 10 39.8 11 20.7 20.5 12 37.2 36,8 13 85.9 - 14 44.5 44.5 15 47.7 47.6 16 154.5 - 17 4.99 5.33 brs brs 104.4 4.99 5.46 br s brs 104.2 18 1.25 s 28.3 1.25 s 28,3 19 176.9 - 20 1,26 s 15.6 1.21 s 15.6 13-O-GSc 1 5.13 d 7.7 99.7 5.00 d 8.4 99.0 2 4.07 dd 7.7/9.0 75.2 74.8 2 4.22 dd 9.0/8.9 78.8 78.2 4 4.32 dd 8.9/8.8 72.3 71.8 5 4.00 ddd 8,8/2.4/4.4 78.0 77.2 8 4.45 4.26 dd 2.4/12.0 dd 2.4/12.0 63.0 4.42 dd 2.3/12.5 62.6 19-O-Gic 1 6.14 d 7.9 95.9 5.99 d 8.1 95.2 2 4.20 dd 7.9/9.0 74.0 73.5 2 4.22 dd 9.0/9.0 79.1 78.4 4 4.09 dd 9.0/8.8 71.1 70.7 5 3.98 ddd 8.8/2.2/4.6 79.3 78.4 5 4.61 4.26 dd 2.2/11.7 dd 4.6/11.7 62.1 4.31 4.22 dd 2.5/12.3 dd 4.0/11.7 61.8

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FIGURE 8 (C'ONTj

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2018200459 19 Jan 2018

Ξ Pellet, 24h Pellet, 99h □ Supernatant, 24h □ Supernatant, 99h

FIGURE 9

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2018200459 19 Jan 2018

FIGURE 10A 25 20 liiil Si RebA 10 Mb Si bub·: 111 * ( SS 19-SMG 5 IIB 0 iili 1·· sup__cont SLip__85c2__p53

FIGURE 10B

Si :<·? bA : 000½ $O? : Τ' ϊϊϊϊϊϊίϊ is Rubu lllOlIllllllll ll! Si 19-SMG pel_cont pel_85c2_p53

2018200459 19 Jan 2018

<110> EVOLVA SA SEQUENCE LISTING <120> RECOMBINANT PRODUCTION OF STEVIOL GLYCOSIDES <130> 25903-0003WO1 <140> <141> <150> 61/471,622 <151> 2011-04-04 <150> 61/434,582 <151> 2011-01-20 <150> 61/350,553 <151> 2010-06-02 <160> 151 <170> PatentIn version 3.5

<210> 1 <211> 460 <212> PRT <213> Stevia rebaudiana <400> 1

Met Ala Glu 1 Gln Gln 5 Lys Ile Lys Lys Ser 10 Pro His Val Leu Leu 15 Ile Pro Phe Pro Leu Gln Gly His Ile Asn Pro Phe Ile Gln Phe Gly Lys 20 25 30 Arg Leu Ile Ser Lys Gly Val Lys Thr Thr Leu Val Thr Thr Ile His 35 40 45 Thr Leu Asn Ser Thr Leu Asn His Ser Asn Thr Thr Thr Thr Ser Ile 50 55 60 Glu Ile Gln Ala Ile Ser Asp Gly cys Asp Glu Gly Gly Phe Met Ser 65 70 75 80 Ala Gly Glu Ser Tyr Leu Gl u Thr Phe Lys Gln Val Gly Ser Lys Ser 85 90 95 Leu Ala Asp Leu Ile Lys Lys Leu Gln Ser Glu Gly Thr Thr Ile Asp 100 105 110 Ala Ile Ile Tyr Asp Ser Met Thr Glu T rp Val Leu Asp Val Ala Ile 115 120 125 Glu Phe Gly Ile Asp Gly Gly Ser Phe Phe Thr Gln Ala cys Val Val 130 135 140 Asn Ser Leu Tyr Tyr His Val His Lys Gly Leu Ile Ser Leu Pro Leu 145 150 155 160

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2018200459 19 Jan 2018

Gly Glu Thr Val Ser Val 165 Pro Gly Phe Pro Val 170 Leu Gln Arg T rp 175 Glu Thr Pro Leu Ile Leu Gln Asn His Glu Gln Ile Gln Ser Pro T rp Ser 180 185 190 Gln Met Leu Phe Gly Gln Phe Ala Asn Ile Asp Gln Ala Arg T rp Val 195 200 205 Phe Thr Asn Ser Phe Tyr Lys Leu Glu Glu Glu Val Ile Glu T rp Thr 210 215 220 Arg Lys Ile T rp Asn Leu Lys Val Ile Gly Pro Thr Leu Pro Ser Met 225 230 235 240 Tyr Leu Asp Lys Arg Leu Asp Asp Asp Lys Asp Asn Gly Phe Asn Leu 245 250 255 Tyr Lys Ala Asn His His Gl u cys Met Asn T rp Leu Asp Asp Lys Pro 260 265 270 Lys Glu Ser Val Val Tyr Val Ala Phe Gly Ser Leu Val Lys His Gly 275 280 285 Pro Glu Gln Val Glu Glu Ile Thr Arg Ala Leu Ile Asp Ser Asp Val 290 295 300 Asn Phe Leu T rp Val Ile Lys His Lys Glu Glu Gly Lys Leu Pro Glu 305 310 315 320 Asn Leu Ser Glu Val Ile Lys Thr Gly Lys Gly Leu Ile Val Ala T rp 325 330 335 cys Lys Gln Leu Asp Val Leu Ala His Glu Ser Val Gly cys Phe Val 340 345 350 Thr His cys Gly Phe Asn Ser Thr Leu Glu Ala Ile Ser Leu Gly Val 355 360 365 Pro Val Val Ala Met Pro Gl n Phe Ser Asp Gln Thr Thr Asn Ala Lys 370 375 380 Leu Leu Asp Glu Ile Leu Gly Val Gly Val Arg Val Lys Ala Asp Glu 385 390 395 400 Asn Gly Ile Val Arg Arg Gly Asn Leu Ala Ser cys Ile Lys Met Ile 405 410 415 Met Glu Glu Glu Arg Gly Val Ile Ile Arg Lys Asn Ala Val Lys T rp 420 425 430

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Lys Asp Leu Ala Lys Val Ala Val His Glu Gly Gly Ser Ser Asp Asn 435 440 445

Asp Ile Val Glu Phe Val Ser Glu Leu Ile Lys Ala 450 455 460 <210> 2 <211> 1383 <212> DNA <213> Stevia rebaudiana <400> 2

atggcagagc aacaaaagat caaaaagtca cctcacgtct tacttattcc atttcctctg 60 caaggacata tcaacccatt catacaattt gggaaaagat tgattagtaa gggtgtaaag 120 acaacactgg taaccactat ccacactttg aattctactc tgaaccactc aaatactact 180 actacaagta tagaaattca agctatatca gacggatgcg atgagggtgg ctttatgtct 240 gccggtgaat cttacttgga aacattcaag caagtgggat ccaagtctct ggccgatcta 300 atcaaaaagt tacagagtga aggcaccaca attgacgcca taatctacga ttctatgaca 360 gagtgggttt tagacgttgc tatcgaattt ggtattgatg gaggttcctt tttcacacaa 420 gcatgtgttg tgaattctct atactaccat gtgcataaag ggttaatctc tttaccattg 480 ggtgaaactg tttcagttcc aggttttcca gtgttacaac gttgggaaac cccattgatc 540 ttacaaaatc atgaacaaat acaatcacct tggtcccaga tgttgtttgg tcaattcgct 600 aacatcgatc aagcaagatg ggtctttact aattcattct ataagttaga ggaagaggta 660 attgaatgga ctaggaagat ctggaatttg aaagtcattg gtccaacatt gccatcaatg 720 tatttggaca aaagacttga tgatgataaa gataatggtt tcaatttgta caaggctaat 780 catcacgaat gtatgaattg gctggatgac aaaccaaagg aatcagttgt atatgttgct 840 ttcggctctc ttgttaaaca tggtccagaa caagttgagg agattacaag agcacttata 900 gactctgacg taaacttttt gtgggtcatt aagcacaaag aggaggggaa actgccagaa 960 aacctttctg aagtgataaa gaccggaaaa ggtctaatcg ttgcttggtg taaacaattg 1020 gatgttttag ctcatgaatc tgtaggctgt tttgtaacac attgcggatt caactctaca 1080 ctagaagcca tttccttagg cgtacctgtc gttgcaatgc ctcagttctc cgatcagaca 1140 accaacgcta aacttttgga cgaaatacta ggggtgggtg tcagagttaa agcagacgag 1200 aatggtatcg tcagaagagg gaacctagct tcatgtatca aaatgatcat ggaagaggaa 1260 agaggagtta tcataaggaa aaacgcagtt aagtggaagg atcttgcaaa ggttgccgtc 1320 catgaaggcg gctcttcaga taatgatatt gttgaatttg tgtccgaact aatcaaagcc 1380

taa 1383 <210> 3 <211> 481 <212> PRT <213> Stevia rebaudiana

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2018200459 19 Jan 2018

<400> 3 Ala 5 Thr Thr Glu Lys Lys 10 Pro His Val Ile Phe 15 Ile Met 1 Asp Ala Met Pro Phe Pro Ala Gln Ser His Ile Lys Ala Met Leu Lys Leu Ala Gln 20 25 30 Leu Leu His His Lys Gly Leu Gln Ile Thr Phe Val Asn Thr Asp Phe 35 40 45 lie His Asn Gln Phe Leu Gl u Ser Ser Gly Pro His cys Leu Asp Gly 50 55 60 Ala Pro Gly Phe Arg Phe Gl u Thr Ile Pro Asp Gly Val Ser His Ser 65 70 75 80 Pro Glu Ala Ser Ile Pro Ile Arg Glu Ser Leu Leu Arg Ser Ile Glu 85 90 95 Thr Asn Phe Leu Asp Arg Phe Ile Asp Leu Val Thr Lys Leu Pro Asp 100 105 110 Pro Pro Thr cys Ile Ile Ser Asp Gly Phe Leu Ser Val Phe Thr Ile 115 120 125 Asp Ala Ala Lys Lys Leu Gly Ile Pro Val Met Met Tyr T rp Thr Leu 130 135 140 Ala Ala cys Gly Phe Met Gly Phe Tyr His Ile His Ser Leu Ile Glu 145 150 155 160 Lys Gly Phe Ala Pro Leu Lys Asp Ala Ser Tyr Leu Thr Asn Gly Tyr 165 170 175 Leu Asp Thr Val Ile Asp Trp Val Pro Gly Met Glu Gly Ile Arg Leu 180 185 190 Lys Asp Phe Pro Leu Asp Trp Ser Thr Asp Leu Asn Asp Lys Val Leu 195 200 205 Met Phe Thr Thr Glu Ala Pro Gln Arg Ser His Lys Val Ser His His 210 215 220 lie Phe His Thr Phe Asp Gl u Leu Glu Pro Ser Ile Ile Lys Thr Leu 225 230 235 240 Ser Leu Arg Tyr Asn His Ile Tyr Thr Ile Gly Pro Leu Gln Leu Leu 245 250 255 Leu Asp Gln Ile Pro Glu Gl u Lys Lys Gln Thr Gly Ile Thr Ser Leu 260 265 270

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His Gly Tyr Ser Leu Val 275 Lys Glu 280 Glu Pro Glu cys Phe 285 Gln Trp Leu Gln Ser Lys Glu Pro Asn Ser Val Val Tyr Val Asn Phe Gly Ser Thr 290 295 300 Thr Val Met Ser Leu Glu Asp Met Thr Glu Phe Gly T rp Gly Leu Ala 305 310 315 320 Asn Ser Asn His Tyr Phe Leu T rp Ile Ile Arg Ser Asn Leu Val Ile 325 330 335 Gly Glu Asn Ala Val Leu Pro Pro Glu Leu Glu Glu His Ile Lys Lys 340 345 350 Arg Gly Phe Ile Ala Ser Trp cys Ser Gln Glu Lys Val Leu Lys His 355 360 365 Pro Ser Val Gly Gly Phe Leu Thr His cys Gly T rp Gly Ser Thr Ile 370 375 380 Glu Ser Leu Ser Ala Gly Val Pro Met Ile cys T rp Pro Tyr Ser T rp 385 390 395 400 Asp Gln Leu Thr Asn cys Arg Tyr Ile cys Lys Glu T rp Glu Val Gly 405 410 415 Leu Glu Met Gly Thr Lys Val Lys Arg Asp Glu Val Lys Arg Leu Val 420 425 430 Gln Glu Leu Met Gly Glu Gly Gly His Lys Met Arg Asn Lys Ala Lys 435 440 445 Asp T rp Lys Glu Lys Ala Arg Ile Ala Ile Ala Pro Asn Gly Ser Ser 450 455 460 Ser Leu Asn Ile Asp Lys Met Val Lys Glu Ile Thr Val Leu Ala Arg

465 470 475 480

Asn <210> 4 <211> 1446 <212> DNA <213> Stevia rebaudiana <400> 4

atggatgcaa tggcaactac tgagaaaaag cctcatgtga tcttcattcc atttcctgca 60 caatctcaca taaaggcaat gctaaagtta gcacaactat tacaccataa gggattacag 120 ataactttcg tgaataccga cttcatccat aatcaatttc tggaatctag tggccctcat 180 tgtttggacg gagccccagg gtttagattc gaaacaattc ctgacggtgt ttcacattcc Page 5 240

2018200459 19 Jan 2018

ccagaggcct ccatcccaat aagagagagt ttactgaggt caatagaaac caactttttg 300 gatcgtttca ttgacttggt cacaaaactt ccagacccac caacttgcat aatctctgat 360 ggctttctgt cagtgtttac tatcgacgct gccaaaaagt tgggtatccc agttatgatg 420 tactggactc ttgctgcatg cggtttcatg ggtttctatc acatccattc tcttatcgaa 480 aagggttttg ctccactgaa agatgcatca tacttaacca acggctacct ggatactgtt 540 attgactggg taccaggtat ggaaggtata agacttaaag attttccttt ggattggtct 600 acagacctta atgataaagt attgatgttt actacagaag ctccacaaag atctcataag 660 gtttcacatc atatctttca cacctttgat gaattggaac catcaatcat caaaaccttg 720 tctctaagat acaatcatat ctacactatt ggtccattac aattacttct agatcaaatt 780 cctgaagaga aaaagcaaac tggtattaca tccttacacg gctactcttt agtgaaagag 840 gaaccagaat gttttcaatg gctacaaagt aaagagccta attctgtggt ctacgtcaac 900 ttcggaagta caacagtcat gtccttggaa gatatgactg aatttggttg gggccttgct 960 aattcaaatc attactttct atggattatc aggtccaatt tggtaatagg ggaaaacgcc 1020 gtattacctc cagaattgga ggaacacatc aaaaagagag gtttcattgc ttcctggtgt 1080 tctcaggaaa aggtattgaa acatccttct gttggtggtt tccttactca ttgcggttgg 1140 ggctctacaa tcgaatcact aagtgcagga gttccaatga tttgttggcc atattcatgg 1200 gaccaactta caaattgtag gtatatctgt aaagagtggg aagttggatt agaaatggga 1260 acaaaggtta aacgtgatga agtgaaaaga ttggttcagg agttgatggg ggaaggtggc 1320 cacaagatga gaaacaaggc caaagattgg aaggaaaaag ccagaattgc tattgctcct 1380 aacgggtcat cctctctaaa cattgataag atggtcaaag agattacagt cttagccaga 1440

aactaa 1446 <210> 5 <211> 473 <212> PRT <213> Stevia rebaudiana

<400> 5 Met Ala Thr 1 Ser Asp Ser Ile Val 5 Asp Asp 10 Arg Lys Gln Leu His 15 Val Ala Thr Phe Pro T rp Leu Ala Phe Gly His Ile Leu Pro Tyr Leu Gln 20 25 30 Leu Ser Lys Leu Ile Ala Gl u Lys Gly His Lys Val Ser Phe Leu Ser 35 40 45 Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile 50 55 60 Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp 65 70 75 80

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Ala Glu Ala Thr Thr Asp Val His Pro Glu 90 Asp Ile Pro Tyr Leu 95 Lys 85 Lys Ala Ser Asp Gly Leu Gl n Pro Glu Val Thr Arg Phe Leu Glu Gln 100 105 110 His Ser Pro Asp T rp Ile Ile Tyr Asp Tyr Thr His Tyr T rp Leu Pro 115 120 125 Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr 130 135 140 Thr Pro T rp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile 145 150 155 160 Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro 165 170 175 Lys T rp Phe Pro Phe Pro Thr Lys Val cys T rp Arg Lys His Asp Leu 180 185 190 Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg 195 200 205 Met Gly Leu Val Leu Lys Gly Ser Asp cys Leu Leu Ser Lys cys Tyr 210 215 220 His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln 225 230 235 240 Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp 245 250 255 Glu Lys Asp Glu Thr T rp Val Ser Ile Lys Lys T rp Leu Asp Gly Lys 260 265 270 Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val 275 280 285 Ser Gln Thr Glu Val Val Gl u Leu Ala Leu Gly Leu Glu Leu Ser Gly 290 295 300 Leu Pro Phe Val T rp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser 305 310 315 320 Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg 325 330 335 Gly Leu Val T rp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His 340 345 350

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Glu Ser Val 355 Cys Gly Phe Leu Thr 360 His Cys Gly Ser Gly 365 Ser Ile Val Glu Gly Leu Met Phe Gly Hi s Pro Leu Ile Met Leu Pro Ile Phe Gly 370 375 380 Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile 385 390 395 400 Glu Ile Pro Arg Asn Glu Gl u Asp Gly Cys Leu Thr Lys Glu Ser Val 405 410 415 Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr 420 425 430 Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val 435 440 445 Glu Lys Glu Tyr Val Ser Gl n Phe Val Asp Tyr Leu Glu Lys Asn Ala 450 455 460 Arg Ala Val Ala Ile Asp His Glu Ser 465 470

<210> 6 <211> 1422 <212> DNA <213> Stevia rebaudiana <400> 6

atggctacat ctgattctat tgttgatgac aggaagcagt tgcatgtggc tactttccct 60 tggcttgctt tcggtcatat actgccttac ctacaactat caaaactgat agctgaaaaa 120 ggacataaag tgtcattcct ttcaacaact agaaacattc aaagattatc ttcccacata 180 tcaccattga ttaacgtcgt tcaattgaca cttccaagag tacaggaatt accagaagat 240 gctgaagcta caacagatgt gcatcctgaa gatatccctt acttgaaaaa ggcatccgat 300 ggattacagc ctgaggtcac tagattcctt gagcaacaca gtccagattg gatcatatac 360 gactacactc actattggtt gccttcaatt gcagcatcac taggcatttc tagggcacat 420 ttcagtgtaa ccacaccttg ggccattgct tacatgggtc catccgctga tgctatgatt 480 aacggcagtg atggtagaac taccgttgaa gatttgacaa ccccaccaaa gtggtttcca 540 tttccaacta aagtctgttg gagaaaacac gacttagcaa gactggttcc atacaaggca 600 ccaggaatct cagacggcta tagaatgggt ttagtcctta aagggtctga ctgcctattg 660 tctaagtgtt accatgagtt tgggacacaa tggctaccac ttttggaaac attacaccaa 720 gttcctgtcg taccagttgg tctattacct ccagaaatcc ctggtgatga gaaggacgag 780 acttgggttt caatcaaaaa gtggttagac gggaagcaaa aaggctcagt ggtatatgtg 840 gcactgggtt ccgaagtttt agtatctcaa acagaagttg tggaacttgc cttaggtttg 900 gaactatctg gattgccatt tgtctgggcc tacagaaaac Page 8 caaaaggccc tgcaaagtcc 960

2018200459 19 Jan 2018

gattcagttg aattgccaga cggctttgtc gagagaacta gagatagagg gttggtatgg 1020 acttcatggg ctccacaatt gagaatcctg agtcacgaat ctgtgtgcgg tttcctaaca 1080 cattgtggtt ctggttctat agttgaagga ctgatgtttg gtcatccact tatcatgttg 1140 ccaatctttg gtgaccagcc tttgaatgca cgtctgttag aagataaaca agttggaatt 1200 gaaatcccac gtaatgagga agatggatgt ttaaccaagg agtctgtggc cagatcatta 1260 cgttccgttg tcgttgaaaa ggaaggcgaa atctacaagg ccaatgcccg tgaactttca 1320 aagatctaca atgacacaaa agtagagaag gaatatgttt ctcaatttgt agattaccta 1380 gagaaaaacg ctagagccgt agctattgat catgaatcct aa 1422

<210> 7 <211> 458 <212> PRT <213> Stevia rebaudiana

<400> 7 Asn Lys Thr 5 Glu Thr Thr Val Arg Arg Arg Arg Arg 10 Ile 15 Ile Met 1 Glu Leu Phe Pro Val Pro Phe Gl n Gly His Ile Asn Pro Ile Leu Gln Leu 20 25 30 Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr 35 40 45 Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg 50 55 60 Phe Ile Leu Asp Asn Asp Pro Gln Asp Glu Arg Ile Ser Asn Leu Pro 65 70 75 80 Thr His Gly Pro Leu Ala Gly Met Arg Ile Pro Ile Ile Asn Glu His 85 90 95 Gly Ala Asp Glu Leu Arg Arg Glu Leu Glu Leu Leu Met Leu Ala Ser 100 105 110 Glu Glu Asp Glu Glu Val Ser cys Leu Ile Thr Asp Ala Leu T rp Tyr 115 120 125 Phe Ala Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu 130 135 140 Met Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln 145 150 155 160 Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu 165 170 175 Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Ser Page 9

2018200459 19 Jan 2018

180 185 190 Ala Tyr Ser Asn T rp Gln Ile Leu Lys Glu Ile Leu Gly Lys Met Ile 195 200 205 Lys Gln Thr Lys Ala Ser Ser Gly Val Ile T rp Asn Ser Phe Lys Glu 210 215 220 Leu Glu Glu Ser Glu Leu Gl u Thr Val Ile Arg Glu Ile Pro Ala Pro 225 230 235 240 Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser 245 250 255 Leu Leu Asp His Asp Arg Thr Val Phe Gln T rp Leu Asp Gln Gln Pro 260 265 270 Pro Ser Ser Val Leu Tyr Val Ser Phe Gly Ser Thr Ser Glu Val Asp 275 280 285 Glu Lys Asp Phe Leu Glu Ile Ala Arg Gly Leu Val Asp Ser Lys Gln 290 295 300 Ser Phe Leu T rp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr T rp 305 310 315 320 Val Glu Pro Leu Pro Asp Gly Phe Leu Gly Glu Arg Gly Arg Ile Val 325 330 335 Lys T rp Val Pro Gln Gln Gl u Val Leu Ala His Gly Ala Ile Gly Ala 340 345 350 Phe T rp Thr His Ser Gly Trp Asn Ser Thr Leu Glu Ser Val cys Glu 355 360 365 Gly Val Pro Met Ile Phe Ser Asp Phe Gly Leu Asp Gln Pro Leu Asn 370 375 380 Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu Asn 385 390 395 400 Gly T rp Glu Arg Gly Glu Ile Ala Asn Ala Ile Arg Arg Val Met Val 405 410 415 Asp Glu Glu Gly Glu Tyr Ile Arg Gln Asn Ala Arg Val Leu Lys Gln 420 425 430 Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser Leu 435 440 445 Glu Ser Leu Val Ser Tyr Ile Ser Ser Leu 450 455

Page 10

2018200459 19 Jan 2018 <210> 8 <211> 1377 <212> DNA <213> Stevia rebaudiana <400> 8

atggaaaaca agaccgaaac aacagttaga cgtaggcgta gaatcattct gtttccagta 60 ccttttcaag ggcacatcaa tccaatacta caactagcca acgttttgta ctctaaaggt 120 ttttctatta caatctttca caccaatttc aacaaaccaa aaacatccaa ttacccacat 180 ttcacattca gattcatact tgataatgat ccacaagatg aacgtatttc aaacttacct 240 acccacggtc ctttagctgg aatgagaatt ccaatcatca atgaacatgg tgccgatgag 300 cttagaagag aattagagtt acttatgttg gcatccgaag aggacgagga agtctcttgt 360 ctgattactg acgctctatg gtactttgcc caatctgtgg ctgatagttt gaatttgagg 420 agattggtac taatgacatc cagtctgttt aactttcacg ctcatgttag tttaccacaa 480 tttgacgaat tgggatactt ggaccctgat gacaagacta ggttagagga acaggcctct 540 ggttttccta tgttgaaagt caaagatatc aagtctgcct attctaattg gcaaatcttg 600 aaagagatct taggaaagat gatcaaacag acaaaggctt catctggagt gatttggaac 660 agtttcaaag agttagaaga gtctgaattg gagactgtaa tcagagaaat tccagcacct 720 tcattcctga taccattacc aaaacatttg actgcttcct cttcctcttt gttggatcat 780 gacagaacag tttttcaatg gttggaccaa caaccaccta gttctgtttt gtacgtgtca 840 tttggtagta cttctgaagt cgatgaaaag gacttccttg aaatcgcaag aggcttagtc 900 gatagtaagc agtcattcct ttgggtcgtg cgtccaggtt tcgtgaaagg ctcaacatgg 960 gtcgaaccac ttccagatgg ttttctaggc gaaagaggta gaatagtcaa atgggttcct 1020 caacaggaag ttttagctca tggcgctatt ggggcattct ggactcattc cggatggaat 1080 tcaactttag aatcagtatg cgaaggggta cctatgatct tttcagattt tggtcttgat 1140 caaccactga acgcaagata catgtctgat gttttgaaag tgggtgtata tctagaaaat 1200 ggctgggaaa ggggtgaaat agctaatgca ataagacgtg ttatggttga tgaagagggg 1260 gagtatatca gacaaaacgc aagagtgctg aagcaaaagg ccgacgtttc tctaatgaag 1320 ggaggctctt catacgaatc cttagaatct cttgtttcct acatttcatc actgtaa 1377

<210> 9 <211> 1422 <212> DNA <213> Stevia rebaudiana

<400> 9 atggctacca gtgactccat agttgacgac cgtaagcagc ttcatgttgc gacgttccca 60 tggcttgctt tcggtcacat cctcccttac cttcagcttt cgaaattgat agctgaaaag 120 ggtcacaaag tctcgtttct ttctaccacc agaaacattc aacgtctctc ttctcatatc 180 tcgccactca taaatgttgt tcaactcaca cttccacgtg tccaagagct gccggaggat 240

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2018200459 19 Jan 2018

gcagaggcga ccactgacgt ccaccctgaa gatattccat atctcaagaa ggcttctgat 300 ggtcttcaac cggaggtcac ccggtttcta gaacaacact ctccggactg gattatttat 360 gattatactc actactggtt gccatccatc gcggctagcc tcggtatctc acgagcccac 420 ttctccgtca ccactccatg ggccattgct tatatgggac cctcagctga cgccatgata 480 aatggttcag atggtcgaac cacggttgag gatctcacga caccgcccaa gtggtttccc 540 tttccgacca aagtatgctg gcggaagcat gatcttgccc gactggtgcc ttacaaagct 600 ccggggatat ctgatggata ccgtatgggg ctggttctta agggatctga ttgtttgctt 660 tccaaatgtt accatgagtt tggaactcaa tggctacctc ttttggagac actacaccaa 720 gtaccggtgg ttccggtggg attactgcca ccggaaatac ccggagacga gaaagatgaa 780 acatgggtgt caatcaagaa atggctcgat ggtaaacaaa aaggcagtgt ggtgtacgtt 840 gcattaggaa gcgaggtttt ggtgagccaa accgaggttg ttgagttagc attgggtctc 900 gagctttctg ggttgccatt tgtttgggct tatagaaaac caaaaggtcc cgcgaagtca 960 gactcggtgg agttgccaga cgggttcgtg gaacgaactc gtgaccgtgg gttggtctgg 1020 acgagttggg cacctcagtt acgaatactg agccatgagt cggtttgtgg tttcttgact 1080 cattgtggtt ctggatcaat tgtggaaggg ctaatgtttg gtcaccctct aatcatgcta 1140 ccgatttttg gggaccaacc tctgaatgct cgattactgg aggacaaaca ggtgggaatc 1200 gagataccaa gaaatgagga agatggttgc ttgaccaagg agtcggttgc tagatcactg 1260 aggtccgttg ttgtggaaaa agaaggggag atctacaagg cgaacgcgag ggagctgagt 1320 aaaatctata acgacactaa ggttgaaaaa gaatatgtaa gccaattcgt agactatttg 1380 gaaaagaatg cgcgtgcggt tgccatcgat catgagagtt aa 1422

<210> 10 <211> 473 <212> PRT <213> Stevia rebaudiana

<400> 10 Ser Ile Val Asp Asp Arg 10 Lys Gln Leu His 15 Val Met 1 Ala Thr Ser Asp 5 Ala Thr Phe Pro T rp Leu Ala Phe Gly His Ile Leu Pro Tyr Leu Gln 20 25 30 Leu Ser Lys Leu Ile Ala Gl u Lys Gly His Lys Val Ser Phe Leu Ser 35 40 45 Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile 50 55 60 Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp 65 70 75 80 Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Pro Tyr Leu Lys 85 90 95 Page 12

2018200459 19 Jan 2018

Lys Ala Ser Asp 100 Gly Leu Gln Pro Glu 105 Val Thr Arg Phe Leu 110 Glu Gln His Ser Pro Asp T rp Ile Ile Tyr Asp Tyr Thr His Tyr T rp Leu Pro 115 120 125 Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr 130 135 140 Thr Pro T rp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile 145 150 155 160 Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro 165 170 175 Lys T rp Phe Pro Phe Pro Thr Lys Val cys T rp Arg Lys His Asp Leu 180 185 190 Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg 195 200 205 Met Gly Leu Val Leu Lys Gly Ser Asp cys Leu Leu Ser Lys cys Tyr 210 215 220 His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln 225 230 235 240 Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Val Pro Gly Asp 245 250 255 Glu Lys Asp Glu Thr T rp Val Ser Ile Lys Lys T rp Leu Asp Gly Lys 260 265 270 Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val 275 280 285 Ser Gln Thr Glu Val Val Gl u Leu Ala Leu Gly Leu Glu Leu Ser Gly 290 295 300 Leu Pro Phe Val T rp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser 305 310 315 320 Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg 325 330 335 Gly Leu Val T rp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His 340 345 350 Glu Ser Val cys Gly Phe Leu Thr His cys Gly Ser Gly Ser Ile Val 355 360 365

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2018200459 19 Jan 2018

Glu Gly Leu 370 Met Phe Gly His 375 Pro Leu Ile Met Leu 380 Pro Ile Phe Gly Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile 385 390 395 400 Glu Ile Pro Arg Asn Glu Gl u Asp Gly cys Leu Thr Lys Glu Ser Val 405 410 415 Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr 420 425 430 Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val 435 440 445 Glu Lys Glu Tyr Val Ser Gl n Phe Val Asp Tyr Leu Glu Lys Asn Thr 450 455 460 Arg Ala Val Ala Ile Asp His Glu Ser 465 470

<210> 11 <211> 1422 <212> DNA <213> Stevia rebaudiana <400> 11

atggctacca gtgactccat agttgacgac cgtaagcagc ttcatgttgc gacgttccca 60 tggcttgctt tcggtcacat cctcccttac cttcagcttt cgaaattgat agctgaaaag 120 ggtcacaaag tctcgtttct ttctaccacc agaaacattc aacgtctctc ttctcatatc 180 tcgccactca taaatgttgt tcaactcaca cttccacgtg tccaagagct gccggaggat 240 gcagaggcga ccactgacgt ccaccctgaa gatattccat atctcaagaa ggcttctgat 300 ggtcttcaac cggaggtcac ccggtttcta gaacaacact ctccggactg gattatttat 360 gattatactc actactggtt gccatccatc gcggctagcc tcggtatctc acgagcccac 420 ttctccgtca ccactccatg ggccattgct tatatgggac cctcagctga cgccatgata 480 aatggttcag atggtcgaac cacggttgag gatctcacga caccgcccaa gtggtttccc 540 tttccgacca aagtatgctg gcggaagcat gatcttgccc gactggtgcc ttacaaagct 600 ccggggatat ctgatggata ccgtatgggg ctggttctta agggatctga ttgtttgctt 660 tccaaatgtt accatgagtt tggaactcaa tggctacctc ttttggagac actacaccaa 720 gtaccggtgg ttccggtggg attactgcca ccggaagtac ccggagacga gaaagatgaa 780 acatgggtgt caatcaagaa atggctcgat ggtaaacaaa aaggcagtgt ggtgtacgtt 840 gcattaggaa gcgaggtttt ggtgagccaa accgaggttg ttgagttagc attgggtctc 900 gagctttctg ggttgccatt tgtttgggct tatagaaaac caaaaggtcc cgcgaagtca 960 gactcggtgg agttgccaga cgggttcgtg gaacgaactc gtgaccgtgg gttggtctgg 1020

Page 14

2018200459 19 Jan 2018 acgagttggg cacctcagtt acgaatactg agccatgagt cggtttgtgg tttcttgact cattgtggtt ctggatcaat tgtggaaggg ctaatgtttg gtcaccctct aatcatgcta ccgatttttg gggaccaacc tctgaatgct cgattactgg aggacaaaca ggtgggaatc gagataccaa gaaatgagga agatggttgc ttgaccaagg agtcggttgc tagatcactg aggtccgttg ttgtggaaaa agaaggggag atctacaagg cgaacgcgag ggagctgagt aaaatctata acgacactaa ggttgaaaaa gaatatgtaa gccaattcgt agactatttg gaaaagaata cgcgtgcggt tgccatcgat catgagagtt aa <210> 12 <211> 473 <212> PRT <213> Stevia rebaudiana

<400> 12 Val Asp Asp Arg 10 Lys Gln Leu His 15 Val Met 1 Ala Thr Ser Asp 5 Ser Ile Ala Thr Phe Pro T rp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln 20 25 30 Leu Ser Lys Leu Ile Ala Gl u Lys Gly His Lys Val Ser Phe Leu Ser 35 40 45 Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile 50 55 60 Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp 65 70 75 80 Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys 85 90 95 Lys Ala Val Asp Gly Leu Gl n Pro Glu Val Thr Arg Phe Leu Glu Gln 100 105 110 His Ser Pro Asp T rp Ile Ile Tyr Asp Phe Thr His Tyr T rp Leu Pro 115 120 125 Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala Tyr Phe cys Val Ile 130 135 140 Thr Pro T rp Thr Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile 145 150 155 160 Asn Asp Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro 165 170 175 Lys T rp Phe Pro Phe Pro Thr Lys Val cys T rp Arg Lys His Asp Leu 180 185 190

1080

1140

1200

1260

1320

1380

1422

Page 15

2018200459 19 Jan 2018

Ala Arg Met 195 Glu Pro Tyr Glu Ala Pro Gly Ile Ser Asp Gly Tyr Arg 200 205 Met Gly Met Val Phe Lys Gly Ser Asp cys Leu Leu Phe Lys cys Tyr 210 215 220 His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln 225 230 235 240 Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp 245 250 255 Glu Lys Asp Glu Thr T rp Val Ser Ile Lys Lys T rp Leu Asp Gly Lys 260 265 270 Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val 275 280 285 Ser Gln Thr Glu Val Val Gl u Leu Ala Leu Gly Leu Glu Leu Ser Gly 290 295 300 Leu Pro Phe Val T rp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser 305 310 315 320 Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg 325 330 335 Gly Leu Val T rp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His 340 345 350 Glu Ser Val cys Gly Phe Leu Thr His cys Gly Ser Gly Ser Ile Val 355 360 365 Glu Gly Leu Met Phe Gly Hi s Pro Leu Ile Met Leu Pro Leu Phe Gly 370 375 380 Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile 385 390 395 400 Glu Ile Pro Arg Asn Glu Gl u Asp Gly cys Leu Thr Lys Glu Ser Val 405 410 415 Ala Arg Ser Leu Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr 420 425 430 Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val 435 440 445 Glu Lys Glu Tyr Val Ser Gl n Phe Val Asp Tyr Leu Glu Lys Asn Ala 450 455 460 Arg Ala Val Ala Ile Asp His Glu Ser

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2018200459 19 Jan 2018

465 470 <210> 13 <211> 1422 <212> DNA <213> Stevia rebaudiana <400> 13

atggctacca gtgactccat agttgacgac cgtaagcagc ttcatgttgc gacgttccca 60 tggcttgctt tcggtcacat cctccctttc cttcagcttt cgaaattgat agctgaaaag 120 ggtcacaaag tctcgtttct ttctaccacc agaaacattc aacgtctctc ttctcatatc 180 tcgccactca taaatgttgt tcaactcaca cttccacgtg tccaagagct gccggaggat 240 gcagaggcga ccactgacgt ccaccctgaa gatattcaat atctcaagaa ggctgttgat 300 ggtcttcaac cggaggtcac ccggtttcta gaacaacact ctccggactg gattatttat 360 gattttactc actactggtt gccatccatc gcggctagcc tcggtatctc acgagcctac 420 ttctgcgtca tcactccatg gaccattgct tatttggcac cctcatctga cgccatgata 480 aatgattcag atggtcgaac cacggttgag gatctcacga caccgcccaa gtggtttccc 540 tttccgacca aagtatgctg gcggaagcat gatcttgccc gaatggagcc ttacgaagct 600 ccagggatat ctgatggata ccgtatgggg atggttttta agggatctga ttgtttgctt 660 ttcaaatgtt accatgagtt tggaactcaa tggctacctc ttttggagac actacaccaa 720 gtaccggtgg ttccggtggg attactgccg ccggaaatac ccggagacga gaaagatgaa 780 acatgggtgt caatcaagaa atggctcgat ggtaaacaaa aaggcagtgt ggtgtacgtt 840 gcattaggaa gcgaggcttt ggtgagccaa accgaggttg ttgagttagc attgggtctc 900 gagctttctg ggttgccatt tgtttgggct tatagaaaac caaaaggtcc cgcgaagtca 960 gactcggtgg agttgccaga cgggttcgtg gaacgaactc gtgaccgtgg gttggtctgg 1020 acgagttggg cacctcagtt acgaatactg agccatgagt cggtttgtgg tttcttgact 1080 cattgtggtt ctggatcaat tgtggaaggg ctaatgtttg gtcaccctct aatcatgcta 1140 ccgctttttg gggaccaacc tctgaatgct cgattactgg aggacaaaca ggtgggaatc 1200 gagataccaa gaaatgagga agatggttgc ttgaccaagg agtcggttgc tagatcactg 1260 aggtccgttg ttgtggaaaa cgaaggggag atctacaagg cgaacgcgag ggagctgagt 1320 aaaatctata acgacactaa ggtggaaaaa gaatatgtaa gccaattcgt agactatttg 1380 gaaaagaatg cgcgtgcggt tgccatcgat catgagagtt aa 1422

<210> 14 <211> 485 <212> PRT <213> Stevia rebaudiana <400> 14

Met Tyr Asn Val Thr Tyr His Gln Asn Ser Lys Ala Met Ala Thr Ser 1 5 10 15 Asp Ser Ile Val Asp Asp Arg Lys Gln Leu His Val Ala Thr Phe Pro

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2018200459 19 Jan 2018

20 25 30 T rp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln Leu Ser Lys Leu 35 40 45 Ile Ala Glu Lys Gly His Lys Val Ser Phe Leu Ser Thr Thr Arg Asn 50 55 60 Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile Asn Val Val Gln 65 70 75 80 Leu Thr Leu Pro Arg Val Gl n Glu Leu Pro Glu Asp Ala Glu Ala Thr 85 90 95 Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys Lys Ala Val Asp 100 105 110 Gly Leu Gln Pro Glu Val Thr Arg Phe Leu Glu Gln His Ser Pro Asp 115 120 125 T rp Ile Ile Tyr Asp Phe Thr His Tyr T rp Leu Pro Ser Ile Ala Ala 130 135 140 Ser Leu Gly Ile Ser Arg Ala Tyr Phe cys Val Ile Thr Pro T rp Thr 145 150 155 160 Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile Asn Asp Ser Asp 165 170 175 Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro Lys T rp Phe Pro 180 185 190 Phe Pro Thr Lys Val cys Trp Arg Lys His Asp Leu Ala Arg Met Glu 195 200 205 Pro Tyr Glu Ala Pro Gly Ile Ser Asp Gly Tyr Arg Met Gly Met Val 210 215 220 Phe Lys Gly Ser Asp cys Leu Leu Phe Lys cys Tyr His Glu Phe Gly 225 230 235 240 Thr Gln T rp Leu Pro Leu Leu Glu Thr Leu His Gln Val Pro Val Val 245 250 255 Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp Glu Lys Asp Glu 260 265 270 Thr T rp Val Ser Ile Lys Lys T rp Leu Asp Gly Lys Gln Lys Gly Ser 275 280 285 Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val Ser Gln Thr Glu 290 295 300

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2018200459 19 Jan 2018

Val 305 Val Glu Leu Ala Leu Gly Leu 310 Glu Leu Ser 315 Gly Leu Pro Phe Val 320 T rp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser Asp Ser Val Glu 325 330 335 Leu Pro Asp Gly Phe Val Gl u Arg Thr Arg Asp Arg Gly Leu Val T rp 340 345 350 Thr Ser T rp Ala Pro Gln Leu Arg Ile Leu Ser His Glu Ser Val cys 355 360 365 Gly Phe Leu Thr His cys Gly Ser Gly Ser Ile Val Glu Gly Leu Met 370 375 380 Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe cys Asp Gln Pro Leu 385 390 395 400 Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile Glu Ile Pro Arg 405 410 415 Asn Glu Glu Asp Gly cys Leu Thr Lys Glu Ser Val Ala Arg Ser Leu 420 425 430 Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr Lys Ala Asn Ala 435 440 445 Arg Ala Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val Glu Lys Glu Tyr 450 455 460 Val Ser Gln Phe Val Asp Tyr Leu Glu Lys Asn Ala Arg Ala Val Ala 465 470 475 480 Ile Asp His Glu Ser

485 <210> 15 <211> 1458 <212> DNA <213> Stevia rebaudiana <400> 15

atgtacaacg ttacttatca tcaaaattca aaagcaatgg ctaccagtga ctccatagtt 60 gacgaccgta agcagcttca tgttgcgacg ttcccatggc ttgctttcgg tcacatcctc 120 cctttccttc agctttcgaa attgatagct gaaaagggtc acaaagtctc gtttctttct 180 accaccagaa acattcaacg tctctcttct catatctcgc cactcataaa tgttgttcaa 240 ctcacacttc cacgtgtcca agagctgccg gaggatgcag aggcgaccac tgacgtccac 300 cctgaagata ttcaatatct caagaaggct gttgatggtc ttcaaccgga ggtcacccgg 360 tttctagaac aacactctcc ggactggatt atttatgatt ttactcacta ctggttgcca 420

Page 19

2018200459 19 Jan 2018

tccatcgcgg ctagcctcgg tatctcacga gcctacttct gcgtcatcac tccatggacc 480 attgcttatt tggcaccctc atctgacgcc atgataaatg attcagatgg tcgaaccacg 540 gttgaggatc tcacgacacc gcccaagtgg tttccctttc cgaccaaagt atgctggcgg 600 aagcatgatc ttgcccgaat ggagccttac gaagctccgg ggatatctga tggataccgt 660 atggggatgg tttttaaggg atctgattgt ttgcttttca aatgttacca tgagtttgga 720 actcaatggc tacctctttt ggagacacta caccaagtac cggtggttcc ggtgggatta 780 ctgccgccgg aaatacccgg agacgagaaa gatgaaacat gggtgtcaat caagaaatgg 840 ctcgatggta aacaaaaagg cagtgtggtg tacgttgcat taggaagcga ggctttggtg 900 agccaaaccg aggttgttga gttagcattg ggtctcgagc tttctgggtt gccatttgtt 960 tgggcttata gaaaaccaaa aggtcccgcg aagtcagact cggtggagtt gccagacggg 1020 ttcgtggaac gaactcgtga ccgtgggttg gtctggacga gttgggcacc tcagttacga 1080 atactgagcc acgagtcagt ttgtggtttc ttgactcatt gtggttctgg atcaattgtg 1140 gaagggctaa tgtttggtca ccctctaatc atgctaccga ttttttgtga ccaacctctg 1200 aatgctcgat tactggagga caaacaggtg ggaatcgaga taccaagaaa tgaggaagat 1260 ggttgcttga ccaaggagtc ggttgctaga tcactgaggt ccgttgttgt ggaaaacgaa 1320 ggggagatct acaaggcgaa cgcgagggcg ctgagtaaaa tctataacga cactaaggtg 1380 gaaaaagaat atgtaagcca attcgtagac tatttggaaa agaatgcgcg tgcggttgcc 1440 atcgatcatg agagttaa 1458

<210> 16 <211> 473 <212> PRT <213> Stevia rebaudiana

<400> 16 Ser Ile Val Asp Asp Arg 10 Lys Gln Leu His 15 Val Met 1 Ala Thr Ser Asp 5 Ala Thr Phe Pro T rp Leu Ala Phe Gly His Ile Leu Pro Phe Leu Gln 20 25 30 Leu Ser Lys Leu Ile Ala Gl u Lys Gly His Lys Val Ser Phe Leu Ser 35 40 45 Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile 50 55 60 Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp 65 70 75 80 Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Gln Tyr Leu Lys 85 90 95 Lys Ala Val Asp Gly Leu Gl n Pro Glu Val Thr Arg Phe Leu Glu Gln

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2018200459 19 Jan 2018

100 105 110 His Ser Pro Asp T rp Ile Ile Tyr Asp Phe Thr His Tyr T rp Leu Pro 115 120 125 Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala Tyr Phe cys Val Ile 130 135 140 Thr Pro T rp Thr Ile Ala Tyr Leu Ala Pro Ser Ser Asp Ala Met Ile 145 150 155 160 Asn Asp Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro 165 170 175 Lys T rp Phe Pro Phe Pro Thr Lys Val cys T rp Arg Lys His Asp Leu 180 185 190 Ala Arg Met Glu Pro Tyr Gl u Ala Pro Gly Ile Ser Asp Gly Tyr Arg 195 200 205 Met Gly Met Val Phe Lys Gly Ser Asp cys Leu Leu Phe Lys cys Tyr 210 215 220 His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln 225 230 235 240 Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp 245 250 255 Glu Lys Asp Glu Thr T rp Val Ser Ile Lys Lys T rp Leu Asp Gly Lys 260 265 270 Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Ala Leu Val 275 280 285 Ser Gln Thr Glu Val Val Gl u Leu Ala Leu Gly Leu Glu Leu Ser Gly 290 295 300 Leu Pro Phe Val T rp Ala Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser 305 310 315 320 Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg 325 330 335 Gly Leu Val T rp Thr Ser Trp Ala Pro Gln Leu Arg Ile Leu Ser His 340 345 350 Glu Ser Val cys Gly Phe Leu Thr His cys Gly Ser Gly Ser Ile Val 355 360 365 Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe cys 370 375 380

Page 21

2018200459 19 Jan 2018

Asp 385 Gln Pro Leu Asn Ala Arg 390 Leu Leu Glu Asp 395 Lys Gln Val Gly Ile 400 Glu Ile Pro Arg Asn Glu Gl u Asp Gly cys Leu Thr Lys Glu Ser Val 405 410 415 Ala Arg Ser Leu Arg Ser Val Val Val Glu Asn Glu Gly Glu Ile Tyr 420 425 430 Lys Ala Asn Ala Arg Ala Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val 435 440 445 Glu Lys Glu Tyr Val Ser Gl n Phe Val Asp Tyr Leu Glu Lys Asn Ala 450 455 460 Arg Ala Val Ala Ile Asp His Glu Ser 465 470

<210> 17 <211> 1422 <212> DNA <213> Stevia rebaudiana <400> 17

atggctacca gtgactccat agttgacgac cgtaagcagc ttcatgttgc gacgttccca 60 tggcttgctt tcggtcacat cctccctttc cttcagcttt cgaaattgat agctgaaaag 120 ggtcacaaag tctcgtttct ttctaccacc agaaacattc aacgtctctc ttctcatatc 180 tcgccactca taaatgttgt tcaactcaca cttccacgtg tccaagagct gccggaggat 240 gcagaggcga ccactgacgt ccaccctgaa gatattcaat atctcaagaa ggctgttgat 300 ggtcttcaac cggaggtcac ccggtttcta gaacaacact ctccggactg gattatttat 360 gattttactc actactggtt gccatccatc gcggctagcc tcggtatctc acgagcctac 420 ttctgcgtca tcactccatg gaccattgct tatttggcac cctcatctga cgccatgata 480 aatgattcag atggtcgaac cacggttgag gatctcacga caccgcccaa gtggtttccc 540 tttccgacca aagtatgctg gcggaagcat gatcttgccc gaatggagcc ttacgaagct 600 ccggggatat ctgatggata ccgtatgggg atggttttta agggatctga ttgtttgctt 660 ttcaaatgtt accatgagtt tggaactcaa tggctacctc ttttggagac actacaccaa 720 gtaccggtgg ttccggtggg attactgccg ccggaaatac ccggagacga gaaagatgaa 780 acatgggtgt caatcaagaa atggctcgat ggtaaacaaa aaggcagtgt ggtgtacgtt 840 gcattaggaa gcgaggcttt ggtgagccaa accgaggttg ttgagttagc attgggtctc 900 gagctttctg ggttgccatt tgtttgggct tatagaaaac caaaaggtcc cgcgaagtca 960 gactcggtgg agttgccaga cgggttcgtg gaacgaactc gtgaccgtgg gttggtctgg 1020 acgagttggg cacctcagtt acgaatactg agccacgagt cagtttgtgg tttcttgact 1080 cattgtggtt ctggatcaat tgtggaaggg ctaatgtttg gtcaccctct aatcatgcta 1140

Page 22 ccgatttttt gtgaccaacc tctgaatgct cgattactgg aggacaaaca ggtgggaatc 1200 gagataccaa gaaatgagga agatggttgc ttgaccaagg agtcggttgc tagatcactg 1260 aggtccgttg ttgtggaaaa cgaaggggag atctacaagg cgaacgcgag ggcgctgagt 1320 aaaatctata acgacactaa ggtggaaaaa gaatatgtaa gccaattcgt agactatttg 1380 gaaaagaatg cgcgtgcggt tgccatcgat catgagagtt aa 1422

2018200459 19 Jan 2018 <210> 18 <211> 1086 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 18 atggctttgg taaacccaac cgctcttttc tatggtacct ctatcagaac aagacctaca 60 aacttactaa atccaactca aaagctaaga ccagtttcat catcttcctt accttctttc 120 tcatcagtta gtgcgattct tactgaaaaa catcaatcta atccttctga gaacaacaat 180 ttgcaaactc atctagaaac tcctttcaac tttgatagtt atatgttgga aaaagtcaac 240 atggttaacg aggcgcttga tgcatctgtc ccactaaaag acccaatcaa aatccatgaa 300 tccatgagat actctttatt ggcaggcggt aagagaatca gaccaatgat gtgtattgca 360 gcctgcgaaa tagtcggagg taatatcctt aacgccatgc cagccgcatg tgccgtggaa 420 atgattcata ctatgtcttt ggtgcatgac gatcttccat gtatggataa tgatgacttc 480 agaagaggta aacctatttc acacaaggtc tacggggagg aaatggcagt attgaccggc 540 gatgctttac taagtttatc tttcgaacat atagctactg ctacaaaggg tgtatcaaag 600 gatagaatcg tcagagctat aggggagttg gcccgttcag ttggctccga aggtttagtg 660 gctggacaag ttgtagatat cttgtcagag ggtgctgatg ttggattaga tcacctagaa 720 tacattcaca tccacaaaac agcaatgttg cttgagtcct cagtagttat tggcgctatc 780 atgggaggag gatctgatca gcagatcgaa aagttgagaa aattcgctag atctattggt 840 ctactattcc aagttgtgga tgacattttg gatgttacaa aatctaccga agagttgggg 900 aaaacagctg gtaaggattt gttgacagat aagacaactt acccaaagtt gttaggtata 960 gaaaagtcca gagaatttgc cgaaaaactt aacaaggaag cacaagagca attaagtggc 1020 tttgatagac gtaaggcagc tcctttgatc gcgttagcca actacaatgc gtaccgtcaa 1080 aattga 1086 <210> 19 <211> 1029 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

Page 23

2018200459 19 Jan 2018 <400> 19

atggctgagc aacaaatatc taacttgctg tctatgtttg atgcttcaca tgctagtcag 60 aaattagaaa ttactgtcca aatgatggac acataccatt acagagaaac gcctccagat 120 tcctcatctt ctgaaggcgg ttcattgtct agatacgacg agagaagagt ctctttgcct 180 ctcagtcata atgctgcctc tccagatatt gtatcacaac tatgtttttc cactgcaatg 240 tcttcagagt tgaatcacag atggaaatct caaagattaa aggtggccga ttctccttac 300 aactatatcc taacattacc atcaaaagga attagaggtg cctttatcga ttccctgaac 360 gtatggttgg aggttccaga ggatgaaaca tcagtcatca aggaagttat tggtatgctc 420 cacaactctt cattaatcat tgatgacttc caagataatt ctccacttag aagaggaaag 480 ccatctaccc atacagtctt cggccctgcc caggctatca atactgctac ttacgttata 540 gttaaagcaa tcgaaaagat acaagacata gtgggacacg atgcattggc agatgttacg 600 ggtactatta caactatttt ccaaggtcag gccatggact tgtggtggac agcaaatgca 660 atcgttccat caatacagga atacttactt atggtaaacg ataaaaccgg tgctctcttt 720 agactgagtt tggagttgtt agctctgaat tccgaagcca gtatttctga ctctgcttta 780 gaaagtttat ctagtgctgt ttccttgcta ggtcaatact tccaaatcag agacgactat 840 atgaacttga tcgataacaa gtatacagat cagaaaggct tctgcgaaga tcttgatgaa 900 ggcaagtact cactaacact tattcatgcc ctccaaactg attcatccga tctactgacc 960 aacatccttt caatgagaag agtgcaagga aagttaacgg cacaaaagag atgttggttc 1020

tggaaatga 1029 <210> 20 <211> 903 <212> DNA <213> Artificial Sequence

<220> <223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 20 atggaaaaga ctaaggagaa agcagaacgt atcttgctgg agccatacag atacttatta 60 caactaccag gaaagcaagt ccgttctaaa ctatcacaag cgttcaatca ctggttaaaa 120 gttcctgaag ataagttaca aatcattatt gaagtcacag aaatgctaca caatgcttct 180 ttactgatcg atgatataga ggattcttcc aaactgagaa gaggttttcc tgtcgctcat 240 tccatatacg gggtaccaag tgtaatcaac tcagctaatt acgtctactt cttgggattg 300 gaaaaagtat tgacattaga tcatccagac gctgtaaagc tattcaccag acaacttctt 360 gaattgcatc aaggtcaagg tttggatatc tattggagag acacttatac ttgcccaaca 420 gaagaggagt acaaagcaat ggttctacaa aagactggcg gtttgttcgg acttgccgtt 480 ggtctgatgc aacttttctc tgattacaag gaggacttaa agcctctgtt ggataccttg 540 ggcttgtttt tccagattag agatgactac gctaacttac attcaaagga atattcagaa 600 Page 24

2018200459 19 Jan 2018 aacaaatcat tctgtgaaga tttgactgaa gggaagttta gttttccaac aatccacgcc 660 atttggtcaa gaccagaatc tactcaagtg caaaacattc tgcgtcagag aacagagaat 720 attgacatca aaaagtattg tgttcagtac ttggaagatg ttggttcttt tgcttacaca 780 agacatacac ttagagaatt agaggcaaaa gcatacaagc aaatagaagc ctgtggaggc 840 aatccttctc tagtggcatt ggttaaacat ttgtccaaaa tgttcaccga ggaaaacaag 900 taa 903 <210> 21 <211> 1020 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 21

atggcaagat tctattttct taacgcacta ttgatggtta tctcattaca atcaactaca 60 gccttcactc cagctaaact tgcttatcca acaacaacaa cagctctaaa tgtcgcctcc 120 gccgaaactt ctttcagtct agatgaatac ttggcctcta agataggacc tatagagtct 180 gccttggaag catcagtcaa atccagaatt ccacagaccg ataagatctg cgaatctatg 240 gcctactctt tgatggcagg aggcaagaga attagaccag tgttgtgtat cgctgcatgt 300 gagatgttcg gtggatccca agatgtcgct atgcctactg ctgtggcatt agaaatgata 360 cacacaatgt ctttgattca tgatgatttg ccatccatgg ataacgatga cttgagaaga 420 ggtaaaccaa caaaccatgt cgttttcggc gaagatgtag ctattcttgc aggtgactct 480 ttattgtcaa cttccttcga gcacgtcgct agagaaacaa aaggagtgtc agcagaaaag 540 atcgtggatg ttatcgctag attaggcaaa tctgttggtg ccgagggcct tgctggcggt 600 caagttatgg acttagaatg tgaagctaaa ccaggtacca cattagacga cttgaaatgg 660 attcatatcc ataaaaccgc tacattgtta caagttgctg tagcttctgg tgcagttcta 720 ggtggtgcaa ctcctgaaga ggttgctgca tgcgagttgt ttgctatgaa tataggtctt 780 gcctttcaag ttgccgacga tatccttgat gtaaccgctt catcagaaga tttgggtaaa 840 actgcaggca aagatgaagc tactgataag acaacttacc caaagttatt aggattagaa 900 gagagtaagg catacgcaag acaactaatc gatgaagcca aggaaagttt ggctcctttt 960 ggagatagag ctgccccttt attggccatt gcagatttca ttattgatag aaagaattga 1020

<210> 22 <211> 1068 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 22 atgcacttag caccacgtag agtccctaga ggtagaagat caccacctga cagagttcct 60

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2018200459 19 Jan 2018

gaaagacaag gtgccttggg tagaagacgt ggagctggct ctactggctg tgcccgtgct 120 gctgctggtg ttcaccgtag aagaggagga ggcgaggctg atccatcagc tgctgtgcat 180 agaggctggc aagccggtgg tggcaccggt ttgcctgatg aggtggtgtc taccgcagcc 240 gccttagaaa tgtttcatgc ttttgcttta atccatgatg atatcatgga tgatagtgca 300 actagaagag gctccccaac tgttcacaga gccctagctg atcgtttagg cgctgctctg 360 gacccagatc aggccggtca actaggagtt tctactgcta tcttggttgg agatctggct 420 ttgacatggt ccgatgaatt gttatacgct ccattgactc cacatagact ggcagcagta 480 ctaccattgg taacagctat gagagctgaa accgttcatg gccaatatct tgatataact 540 agtgctagaa gacctgggac cgatacttct cttgcattga gaatagccag atataagaca 600 gcagcttaca caatggaacg tccactgcac attggtgcag ccctggctgg ggcaagacca 660 gaactattag cagggctttc agcatacgcc ttgccagctg gagaagcctt ccaattggca 720 gatgacctgc taggcgtctt cggtgatcca agacgtacag ggaaacctga cctagatgat 780 cttagaggtg gaaagcatac tgtcttagtc gccttggcaa gagaacatgc cactccagaa 840 cagagacaca cattggatac attattgggt acaccaggtc ttgatagaca aggcgcttca 900 agactaagat gcgtattggt agcaactggt gcaagagccg aagccgaaag acttattaca 960 gagagaagag atcaagcatt aactgcattg aacgcattaa cactgccacc tcctttagct 1020 gaggcattag caagattgac attagggtct acagctcatc ctgcctaa 1068

<210> 23 <211> 993 <212> DNA <213> Artificial Sequence

<220> <223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 23 atgtcatatt tcgataacta cttcaatgag atagttaatt ccgtgaacga catcattaag 60 tcttacatct ctggcgacgt accaaaacta tacgaagcct cctaccattt gtttacatca 120 ggaggaaaga gactaagacc attgatcctt acaatttctt ctgatctttt cggtggacag 180 agagaaagag catactatgc tggcgcagca atcgaagttt tgcacacatt cactttggtt 240 cacgatgata tcatggatca agataacatt cgtagaggtc ttcctactgt acatgtcaag 300 tatggcctac ctttggccat tttagctggt gacttattgc atgcaaaagc ctttcaattg 360 ttgactcagg cattgagagg tctaccatct gaaactatca tcaaggcgtt tgatatcttt 420 acaagatcta tcattatcat atcagaaggt caagctgtcg atatggaatt cgaagataga 480 attgatatca aggaacaaga gtatttggat atgatatctc gtaaaaccgc tgccttattc 540 tcagcttctt cttccattgg ggcgttgata gctggagcta atgataacga tgtgagatta 600 atgtccgatt tcggtacaaa tcttgggatc gcatttcaaa ttgtagatga tatacttggt 660 ttaacagctg atgaaaaaga gctaggaaaa cctgttttca gtgatatcag agaaggtaaa 720

Page 26 aagaccatat tagtcattaa gactttagaa ttgtgtaagg aagacgagaa aaagattgtg 780 ttaaaagcgc taggcaacaa gtcagcatca aaggaagagt tgatgagttc tgctgacata 840 atcaaaaagt actcattgga ttacgcctac aacttagctg agaaatacta caaaaacgcc 900 atcgattctc taaatcaagt ttcaagtaaa agtgatattc cagggaaggc attgaaatat 960 cttgctgaat tcaccatcag aagacgtaag taa 993

2018200459 19 Jan 2018 <210> 24 <211> 894 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 24 atggtcgcac aaactttcaa cctggatacc tacttatccc aaagacaaca acaagttgaa 60 gaggccctaa gtgctgctct tgtgccagct tatcctgaga gaatatacga agctatgaga 120 tactccctcc tggcaggtgg caaaagatta agacctatct tatgtttagc tgcttgcgaa 180 ttggcaggtg gttctgttga acaagccatg ccaactgcgt gtgcacttga aatgatccat 240 acaatgtcac taattcatga tgacctgcca gccatggata acgatgattt cagaagagga 300 aagccaacta atcacaaggt gttcggggaa gatatagcca tcttagcggg tgatgcgctt 360 ttagcttacg cttttgaaca tattgcttct caaacaagag gagtaccacc tcaattggtg 420 ctacaagtta ttgctagaat cggacacgcc gttgctgcaa caggcctcgt tggaggccaa 480 gtcgtagacc ttgaatctga aggtaaagct atttccttag aaacattgga gtatattcac 540 tcacataaga ctggagcctt gctggaagca tcagttgtct caggcggtat tctcgcaggg 600 gcagatgaag agcttttggc cagattgtct cattacgcta gagatatagg cttggctttt 660 caaatcgtcg atgatatcct ggatgttact gctacatctg aacagttggg gaaaaccgct 720 ggtaaagacc aggcagccgc aaaggcaact tatccaagtc tattgggttt agaagcctct 780 agacagaaag cggaagagtt gattcaatct gctaaggaag ccttaagacc ttacggttca 840 caagcagagc cactcctagc gctggcagac ttcatcacac gtcgtcagca ttaa 894 <210> 25 <211> 1116 <212> DNA <213> Artificial Sequence

<220> <223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 25 atggcctctg ttactttggg ttcctggatc gtcgtccacc accataacca tcaccatcca 60 tcatctatcc taactaaatc tcgttcaaga tcctgtccta ttacactaac caaaccaatc 120 tcttttcgtt caaagagaac agtttcctct agtagttcta tcgtgtcctc tagtgtcgtc 180

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2018200459 19 Jan 2018

actaaggaag acaatctgag acagtctgaa ccttcttcct ttgatttcat gtcatatatc 240 attactaagg cagaactagt gaataaggct cttgattcag cagttccatt aagagagcca 300 ttgaaaatcc atgaagcaat gagatactct cttctagctg gcgggaagag agtcagacct 360 gtactctgca tagcagcgtg cgaattagtt ggtggcgagg aatcaaccgc tatgcctgcc 420 gcttgtgctg tagaaatgat tcatacaatg tcactgatac acgatgattt gccatgtatg 480 gataacgatg atctgagaag gggtaagcca actaaccata aggttttcgg cgaagatgtt 540 gccgtcttag ctggtgatgc tttgttatct ttcgcgttcg aacatttggc atccgcaaca 600 tcaagtgatg ttgtgtcacc agtaagagta gttagagcag ttggagaact ggctaaagct 660 attggaactg agggtttagt tgcaggtcaa gtcgtcgata tctcttccga aggtcttgat 720 ttgaatgatg taggtcttga acatctcgaa ttcatccatc ttcacaagac agctgcactt 780 ttagaagcca gtgcggttct cggcgcaatt gttggcggag ggagtgatga cgaaattgag 840 agattgagga agtttgctag atgtatagga ttactgttcc aagtagtaga cgatatacta 900 gatgtgacaa agtcttccaa agagttggga aaaacagctg gtaaagattt gattgccgac 960 aaattgacct accctaagat tatggggcta gaaaaatcaa gagaatttgc cgagaaactc 1020 aatagagagg cgcgtgatca actgttgggt ttcgattctg ataaagttgc accactctta 1080 gccttagcca actacatcgc ttacagacaa aactaa 1116

<210> 26 <211> 1086 <212> DNA <213> Stevia rebaudiana

<400> 26 atggctcttg taaatcccac agctttgttc tatggaacct ccataagaac cagacccaca 60 aacttgctca acccgaccca aaaacttcga cccgtttcct cgtcttcttt gccttccttc 120 tcttcagttt ctgcaatctt gacggaaaaa caccaatcaa acccatcaga aaacaataac 180 ttgcaaaccc atctcgaaac accattcaat ttcgactctt acatgctgga gaaagtaaac 240 atggtgaatg aagctctgga cgcctcggtt ccactcaaag acccgataaa gatccatgaa 300 tccatgcggt actcccttct agctggcggg aaacgcatcc gaccgatgat gtgcatcgcc 360 gcttgcgaaa tagtcggagg caacatatta aacgccatgc cagctgcatg cgcggtcgag 420 atgattcaca ccatgtcact agttcatgac gaccttccat gcatggataa cgacgacttc 480 cgacgtggaa aaccaataag ccacaaggtg tacggtgaag aaatggcggt tctaaccggg 540 gacgcgttac tctcattatc cttcgaacat atcgcgaccg cgacaaaagg cgtatccaaa 600 gacaggatcg tccgagccat tggtgaactc gcaaggtccg ttggctcgga gggtttggtc 660 gccggtcagg tggttgatat tttatccgaa ggggctgatg ttgggttaga ccacttggag 720 tatattcata tacacaagac tgcaatgttg cttgagagct cggtcgtgat cggcgcgatc 780 atgggcggtg ggtctgacca acagatcgaa aagttgcgaa agtttgcgag atcgattggt 840 ttgttgtttc aggtggtaga tgatattctt gatgtcacaa agtcgactga ggaattgggg 900

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2018200459 19 Jan 2018

aaaacggcgg gaaaagattt gctgacggac aagacaacgt atccgaagtt gttggggatc 960 gaaaaatcga gagaatttgc ggagaaatta aacaaggaag cgcaagaaca attgtcgggg 1020 tttgatcgcc gcaaggcggc tccgttaatt gcccttgcta attacaatgc ttataggcaa 1080 aactga 1086 <210> 27 <211> 1029 <212> DNA <213> Gibberella fujikuroi <400> 27 atggctgaac aacagatctc caaccttctt tcaatgtttg atgcttctca cgcaagccag 60 aagttggaga ttacggttca gatgatggat acctaccatt acagagaaac tcctccagac 120 tcttcctctt cagaaggcgg ttccttatct cgctatgatg agcgacgggt ctcccttccg 180 ctctctcaca atgcagcctc cccagacata gtctcccagt tatgcttctc aacagctatg 240 agctcggagc tcaatcacag gtggaagtca cagcgcctca aggttgctga ctctccctac 300 aactacatcc tgactcttcc atctaaaggt attcgtgggg ctttcattga ctcactgaat 360 gtctggctcg aggtccccga agacgagacc tcggtgatca aagaggtgat tggcatgctc 420 cacaactcgt ctctcataat cgatgacttc caagacaact ccccacttcg gcggggcaag 480 ccatctacac atactgtctt cggtccagca caagcaatca acacagcaac atatgtcatc 540 gtcaaggcca tcgagaaaat acaggatatc gtcggtcacg atgcattggc agatgtaact 600 ggcactataa ccacaatctt ccagggtcag gcaatggatc tgtggtggac tgctaatgcc 660 attgttccgt ctatccaaga atatctcctg atggtcaatg acaagactgg tgccctgttc 720 aggttatcgc ttgaactact ggcgctgaac tctgaagcat ccatcagtga cagcgcgctt 780 gaatctctca gcagcgctgt ctcactgctc gggcagtatt tccagataag agatgattac 840 atgaatctca ttgacaacaa gtatactgat cagaaaggat tttgcgagga tctggacgag 900 gggaaatact cgttgactct aatccatgct ctgcagaccg actccagcga ccttctcacc 960 aacatcttat cgatgagaag agtccaagga aaacttacgg cgcagaaaag atgctggttt 1020

tggaagtga 1029 <210> 28 <211> 903 <212> DNA <213> Mus musculus <400> 28 atggagaaaa ctaaagagaa agctgagagg attcttctag agccctatag gtacttactt 60 cagttaccag gtaaacaggt gagaagcaaa ctttcacagg catttaatca ctggctgaaa 120 gttccagaag acaagctaca gattatcatt gaagtgactg aaatgttgca taatgccagt 180 ttactcattg atgatattga agacagttca aagctccgac gtggtttccc agtggctcac 240 agcatctatg gtgtcccatc tgtcattaat tctgccaatt acgtctactt ccttggactg 300 gaaaaagtct taacccttga tcacccggat gcggtgaagc ttttcacacg ccagcttctg 360

Page 29

2018200459 19 Jan 2018 gaacttcatc agggacaagg cctcgatatt tactggaggg acacctacac ttgtccaact 420 gaagaagaat ataaagccat ggtgttgcag aagacaggtg gtttgtttgg attagcagta 480 ggtcttatgc agctgttctc tgattacaaa gaagatctaa agccactgct tgacacactt 540 gggctctttt tccagattag agatgattat gccaatctac actccaaaga atacagtgaa 600 aacaaaagtt tctgtgaaga cttgacagaa gggaagttct cattccccac tatccatgcc 660 atttggtcaa ggccagaaag cacccaggta cagaacatcc tgcgccagag aacagagaat 720 atagatatta aaaagtattg tgtgcagtac ctggaggatg taggttcttt tgcatacact 780 cgacacactc ttagagagct tgaagctaaa gcctacaaac aaattgaggc ctgtggtggg 840 aacccttcac tagtggcttt agtcaagcac ttaagtaaga tgttcacaga agaaaataaa 900 taa 903 <210> 29 <211> 1020 <212> DNA <213> Thalassiosira pseudonana

<400> 29 atggctcgtt tctacttcct gaacgctctc ctcatggtga tttctttaca aagcaccacg 60 gcattcaccc cggcaaaact cgcctaccca acaaccacca ctgcattaaa cgttgcctct 120 gccgaaacat catttagcct cgatgaatac ctagcctcca aaatcggacc cattgaatca 180 gctctcgagg catctgtcaa atctcgcatt cctcaaactg acaagatatg cgagtctatg 240 gcatactcac tcatggctgg aggaaagcgt atccgtcccg ttttgtgcat tgctgcttgt 300 gaaatgtttg ggggaagtca agatgtggct atgccgacgg ctgtggcttt ggagatgatt 360 catactatga gtcttattca tgacgatttg ccttcaatgg acaacgatga tctccgacga 420 ggaaagccaa ctaatcatgt tgtctttgga gaggatgttg ctattcttgc tggggattct 480 cttctcagta cgtcttttga acatgttgcc cgtgaaacca aaggagtgtc agctgaaaag 540 attgtagatg ttatcgctcg cctcgggaag tctgtgggtg cagagggtct tgctggtgga 600 caggttatgg atcttgagtg tgaggcgaag ccaggaacta ccctcgacga tctcaagtgg 660 attcacattc acaaaactgc cactcttctt caagtggcag tggcatcagg tgctgttctt 720 ggaggggcca caccagagga ggttgctgct tgtgaactgt tcgcaatgaa tattggactt 780 gccttccagg tcgctgatga tattttggac gtgacggcat cgagtgagga tcttggcaaa 840 actgctggaa aggatgaagc cacagataag acaacttatc ctaagctttt gggattggag 900 gagagtaagg catacgctcg acaactcata gacgaagcaa aggaatcttt ggctcctttc 960 ggtgatcgtg ctgctccatt gttggcaatt gccgacttta tcattgatcg aaagaactag 1020

<210> 30 <211> 1068 <212> DNA <213> Streptomyces clavuligerus <400> 30

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2018200459 19 Jan 2018

atgcacctgg ctccccgccg agtaccgcgc ggccgtcgaa gcccacctga ccgcgttcct 60 gaacgccaag gagcgctcgg ccgccgccgg ggggccggtt ccacaggatg tgcccgcgct 120 gctgcgggag ttcatcggcg ccgggggggg ggggaagcgg atccgtccgc tgctgtgcat 180 cgcggctggc aggccggcgg cggaacagga ctgccggacg aggtggtgtc cacagcggcg 240 gcgctggaga tgttccacgc gttcgcgctg atccacgacg acatcatgga tgactccgcg 300 accaggcgcg gcagcccgac ggtgcaccgg gcactcgccg accggctcgg cgccgctctc 360 gaccccgacc aagccggaca actgggggtg agcacggcga tcctcgtcgg ggacctcgcc 420 ctgacctggt cggacgaact gctgtacgct cccctgaccc cccaccggct ggccgcggta 480 ctgcccctgg tcacggccat gcgcgcggaa acggtccacg gccagtacct ggacatcacc 540 tccgcccgcc ggcccggcac ggacacctca ctggcgctgc gaatcgcgcg ctacaaaacc 600 gctgcttaca ccatggaacg ccccctgcac atcggagcag cgctcgccgg cgcacgaccg 660 gaactcctgg cagggctcag cgcctacgcg ctgccggcgg gcgaggcatt ccagctcgcc 720 gacgacctcc tgggagtgtt cggcgatcca cggagaaccg gcaaacccga cctcgacgac 780 ctccgcggcg gcaagcacac cgtcctcgtg gccctcgccc gggaacacgc cacacctgaa 840 cagcggcaca ccctggacac cctgctcggc acaccaggcc tcgaccggca gggcgcgtcc 900 cggctgcgct gcgtcctcgt cgccaccggg gcccgggcgg aagccgaacg cctgatcacc 960 gaacggcgcg accaggccct caccgcgctc aacgccctga cactgccccc accgctcgcc 1020 gaggcactcg cccgcctcac cctcgggagt accgcacacc cggcctga 1068

<210> 31 <211> 993 <212> DNA <213> Sulfulobus acidicaldarius <400> 31 atgagttact ttgacaacta ttttaatgag attgttaatt ctgtaaacga cattattaag 60 agctatatat ctggagatgt tcctaaacta tatgaagcct catatcattt gtttacatct 120 ggaggtaaga ggttaagacc attaatctta actatatcat cagatttatt cggaggacag 180 agagaaagag cttattatgc aggtgcagct attgaagttc ttcatacttt tacgcttgtg 240 catgatgata ttatggatca agataatatc agaagagggt tacccacagt ccacgtgaaa 300 tacggcttac ccttagcaat attagctggg gatttactac atgcaaaggc ttttcagctc 360 ttaacccagg ctcttagagg tttgccaagt gaaaccataa ttaaggcttt cgatattttc 420 actcgttcaa taataattat atccgaagga caggcagtag atatggaatt tgaggacaga 480 attgatataa aggagcagga ataccttgac atgatctcac gtaagacagc tgcattattc 540 tcggcatcct caagtatagg cgcacttatt gctggtgcta atgataatga tgtaagactg 600 atgtctgatt tcggtacgaa tctaggtatt gcatttcaga ttgttgacga tatcttaggt 660 ctaacagcag acgaaaagga acttggaaag cctgttttta gtgatattag ggagggtaaa 720 aagactatac ttgtaataaa aacactggag ctttgtaaag aggacgagaa gaagattgtc 780

Page 31 ctaaaggcgt taggtaataa gtcagcctca aaagaagaat taatgagctc agcagatata 840 attaagaaat actctttaga ttatgcatac aatttagcag agaaatatta taaaaatgct 900 atagactctt taaatcaagt ctcctctaag agtgatatac ctggaaaggc tttaaaatat 960 ctagctgaat ttacgataag aaggagaaaa taa 993

2018200459 19 Jan 2018 <210> 32 <211> 894 <212> DNA <213> Synechococcus sp.

<400> 32 ttggttgccc aaaccttcaa cctggacacc tacttgagcc aacgccagca acaggtggaa 60 gaggcgcttt ctgcggcatt ggttcccgcc tatccggagc gcatttacga ggcgatgcgc 120 tacagcctgc tggcgggggg gaaacgcctg aggccgatcc tctgtctggc ggcctgtgag 180 ttggccggcg gctctgtgga gcaggccatg cccaccgcct gcgccctgga gatgatccac 240 accatgtcgc tgatccacga cgatctgccg gcgatggaca acgacgattt tcgccgcggc 300 aagcccacca atcacaaggt attcggcgag gatatcgcca ttttggcagg agatgccctg 360 ttggcctatg cctttgagca tatcgccagc caaacgcggg gggtgccgcc gcagttggtg 420 ctgcaagtca ttgcccgcat tggccatgct gtggcggcaa ccggcttggt agggggccag 480 gtggtggatc tggagtccga aggcaaagcc atttccctag aaactttgga gtacatccac 540 agtcacaaga cgggtgctct gctggaggcc tcggtggttt cgggagggat cctggcaggg 600 gccgatgagg agctgctggc gcggctgagc cactacgctc gggacatcgg cctggctttt 660 cagatcgtgg acgacatttt ggatgttact gccaccagcg agcaactggg caaaacggca 720 ggcaaggatc aagctgccgc caaagccacc taccccagct tgttgggcct agaggcttcc 780 cggcagaaag ctgaggaact gatccaatcg gccaaggagg cgttgcgccc ctacggatcc 840 caggccgagc ccctgttggc tctggccgat ttcatcaccc gccgccagca ttga 894

<210> 33 <211> 1116 <212> DNA <213> Arabidopsis thaliana <400> 33 atggcttcag tgactctagg ttcatggatt gttgttcacc accacaatca tcatcatcca 60 tcttcaatcc ttaccaaatc cagatccaga tcttgtccta taactcttac taaacccatc 120 tcctttcgat caaaacgcac cgtttcatca tcttcttcaa tcgtttcttc ttccgttgtt 180 acaaaagaag acaatctacg ccaatctgaa ccatcctctt tcgatttcat gtcgtacatc 240 atcaccaaag ccgaattagt caacaaagct ttagattcag ctgttcctct ccgtgagcca 300 ctcaagatcc acgaagcgat gcgttactct cttctcgccg gtggcaaaag agttagacca 360 gttctctgca tcgctgcttg tgaactcgtc ggaggtgaag aatcaaccgc tatgccagca 420 gcttgcgccg tcgagatgat tcacaccatg tcgttgatcc acgacgatct cccttgtatg 480 gataacgacg atctccgccg tggaaaaccg accaaccaca aagtgtttgg tgaagacgtc 540

Page 32

2018200459 19 Jan 2018 gctgttttag ccggagacgc gcttctctct ttcgctttcg agcatttagc ttcggcgacg 600 agttctgatg ttgtttctcc ggtgagagtg gttcgagccg ttggagaatt ggctaaagcg 660 ataggaacag aagggttagt ggcgggtcaa gtcgtggata ttagtagtga agggttagat 720 ttaaacgacg tcggtttaga gcatttggag tttatccatt tgcataaaac ggcggcgttg 780 cttgaagctt ctgctgtttt gggagctatt gttggtggag gaagtgatga tgagattgag 840 aggttaagaa agtttgcgag atgtattggt ttgttgtttc aggtggttga tgatatcttg 900 gatgtgacga aatcgtcgaa agagttaggg aaaactgctg ggaaagattt gattgctgat 960 aagttgacgt atcctaagat tatgggtttg gagaaatcga gagagtttgc tgagaaattg 1020 aatagagagg ctcgtgatca gcttttaggg tttgattctg ataaggttgc tcctttgttg 1080 gctttggcta attacattgc ctatagacag aactga 1116 <210> 34 <211> 2364 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 34

atgaaaaccg ggtttatctc accagcaaca gtatttcatc acagaatctc accagcgacc 60 actttcagac atcacttatc acctgctact acaaactcta caggcattgt cgccttaaga 120 gacatcaact tcagatgtaa agcagtttct aaagagtact ctgatctgtt gcagaaagat 180 gaggcttctt tcacaaaatg ggacgatgac aaggtgaaag atcatcttga taccaacaaa 240 aacttatacc caaatgatga gattaaggaa tttgttgaat cagtaaaggc tatgttcggt 300 agtatgaatg acggggagat aaacgtctct gcatacgata ctgcatgggt tgctttggtt 360 caagatgtcg atggatcagg tagtcctcag ttcccttctt ctttagaatg gattgccaac 420 aatcaattgt cagatggatc atggggagat catttgctgt tctcagctca cgatagaatc 480 atcaacacat tagcatgcgt tattgcactt acaagttgga atgttcatcc ttctaagtgt 540 gaaaaaggtt tgaattttct gagagaaaac atttgcaaat tagaagatga aaacgcagaa 600 catatgccaa ttggttttga agtaacattc ccatcactaa ttgatatcgc gaaaaagttg 660 aacattgaag tacctgagga tactccagca cttaaagaga tctacgcacg tagagatatc 720 aagttaacta agatcccaat ggaagttctt cacaaggtac ctactacttt gttacattct 780 ttggaaggaa tgcctgattt ggagtgggaa aaactgttaa agctacaatg taaagatggt 840 agtttcttgt tttccccatc tagtaccgca ttcgccctaa tgcaaacaaa agatgagaaa 900 tgcttacagt atctaacaaa tatcgtcact aagttcaacg gtggcgtgcc taatgtgtac 960 ccagtcgatt tgtttgaaca tatttgggtt gttgatagac tgcagagatt ggggattgcc 1020 agatacttca aatcagagat aaaagattgt gtagagtata tcaataagta ctggaccaaa 1080 aatggaattt gttgggctag aaatactcac gttcaagata Page 33 tcgatgatac agccatggga 1140

2018200459 19 Jan 2018

ttcagagtgt tgagagcgca cggttatgac gtcactccag atgtttttag acaatttgaa 1200 aaagatggta aattcgtttg ctttgcaggg caatcaacac aagccgtgac aggaatgttt 1260 aacgtttaca gagcctctca aatgttgttc ccaggggaga gaattttgga agatgccaaa 1320 aagttctctt acaattactt aaaggaaaag caaagtacca acgaattgct ggataaatgg 1380 ataatcgcta aagatctacc tggtgaagtt ggttatgctc tggatatccc atggtatgct 1440 tccttaccaa gattggaaac tcgttattac cttgaacaat acggcggtga agatgatgtc 1500 tggataggca agacattata cagaatgggt tacgtgtcca ataacacata tctagaaatg 1560 gcaaagctgg attacaataa ctatgttgca gtccttcaat tagaatggta cacaatacaa 1620 caatggtacg tcgatattgg tatagagaag ttcgaatctg acaacatcaa gtcagtcctg 1680 gtttcttact acttggctgc ggcttcaata ttcgaacctg agagatctaa ggagagaatc 1740 gcttgggcaa agacaacaat cttagtcgat aagatcacat caattttcga ttcctctcag 1800 tcaagtaagg aagatattac tgcctttatt gacaagtttc gtaacaagtc ctcctctaaa 1860 aagcactcta tcaacggtga accatggcat gaagttatgg tagctttgaa aaagacctta 1920 cacggctttg ctctggatgc tcttatgact cattctcaag atatacatcc acagttacat 1980 caagcctggg aaatgtggtt gactaaacta caagacggcg tagatgttac tgctgagcta 2040 atggtccaaa tgatcaacat gactgctggc agatgggtat caaaggaatt acttactcat 2100 ccacaatatc aaagattgtc tactgtgaca aattctgtgt gtcacgatat taccaaactt 2160 cacaatttca aggagaattc caccacagtg gattcaaagg ttcaggaact agtccagttg 2220 gtttttagtg acacaccaga tgatttggat caagatatga aacaaacatt cctgacagtg 2280 atgaagacat tctactacaa ggcgtggtgt gatccaaaca ctataaacga tcatatatct 2340 aaagttttcg aaatcgtaat ttga 2364

<210> 35 <211> 1584 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 35 atgcctgatg cacacgatgc tccacctcca caaataagac agagaacact agtagatgag 60 gctacccaac tgctaactga gtccgcagaa gatgcatggg gtgaagtcag tgtgtcagaa 120 tacgaaacag caaggctagt tgcccatgct acatggttag gtggacacgc cacaagagtg 180 gccttccttc tggagagaca acacgaagac gggtcatggg gtccaccagg tggatatagg 240 ttagtcccta cattatctgc tgttcacgca ttattgacat gtcttgcctc tcctgctcag 300 gatcatggcg ttccacatga tagactttta agagctgttg acgcaggctt gactgccttg 360 agaagattgg ggacatctga ctccccacct gatactatag cagttgagct ggttatccca 420 tctttgctag agggcattca acacttactg gaccctgctc atcctcatag tagaccagcc 480

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2018200459 19 Jan 2018

ttctctcaac atagaggctc tcttgtttgt cctggtggac tagatgggag aactctagga 540 gctttgagat cacacgccgc agcaggtaca ccagtaccag gaaaagtctg gcacgcttcc 600 gagactttgg gcttgagtac cgaagctgct tctcacttgc aaccagccca aggtataatc 660 ggtggctctg ctgctgccac agcaacatgg ctaaccaggg ttgcaccatc tcaacagtca 720 gattctgcca gaagatacct tgaggaatta caacacagat actctggccc agttccttcc 780 attaccccta tcacatactt cgaaagagca tggttattga acaattttgc agcagccggt 840 gttccttgtg aggctccagc tgctttgttg gattccttag aagcagcact tacaccacaa 900 ggtgctcctg ctggagcagg attgcctcca gatgctgatg atacagccgc tgtgttgctt 960 gcattggcaa cacatgggag aggtagaaga ccagaagtac tgatggatta caggactgac 1020 gggtatttcc aatgctttat tggggaaagg actccatcaa tttcaacaaa cgctcacgta 1080 ttggaaacat tagggcatca tgtggcccaa catccacaag atagagccag atacggatca 1140 gccatggata ccgcatcagc ttggctgctg gcagctcaaa agcaagatgg ctcttggtta 1200 gataaatggc atgcctcacc atactacgct actgtttgtt gcacacaagc cctagccgct 1260 catgcaagtc ctgcaactgc accagctaga cagagagctg tcagatgggt tttagccaca 1320 caaagatccg atggcggttg gggtctatgg cattcaactg ttgaagagac tgcttatgcc 1380 ttacagatct tggccccacc ttctggtggt ggcaatatcc cagtccaaca agcacttact 1440 agaggcagag caagattgtg tggagccttg ccactgactc ctttatggca tgataaggat 1500 ttgtatactc cagtaagagt agtcagagct gccagagctg ctgctctgta cactaccaga 1560 gatctattgt taccaccatt gtaa 1584

<210> 36 <211> 1551 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 36 atgaacgccc tatccgaaca cattttgtct gaattgagaa gattattgtc tgaaatgagt 60 gatggcggat ctgttggtcc atctgtgtat gatacggccc aggccctaag attccacggt 120 aacgtaacag gtagacaaga tgcatatgct tggttgatcg cccagcaaca agcagatgga 180 ggttggggct ctgccgactt tccactcttt agacatgctc caacatgggc tgcacttctc 240 gcattacaaa gagctgatcc acttcctggc gcagcagacg cagttcagac cgcaacaaga 300 ttcttgcaaa gacaaccaga tccatacgct catgccgttc ctgaggatgc ccctattggt 360 gctgaactga tcttgcctca gttttgtgga gaggctgctt ggttgttggg aggtgtggcc 420 ttccctagac acccagccct attaccatta agacaggctt gtttagtcaa actgggtgca 480 gtcgccatgt tgccttcagg acacccattg ctccactcct gggaggcatg gggtacttct 540 ccaacaacag cctgtccaga cgatgatggt tctataggta tctcaccagc agctacagcc 600

Page 35

2018200459 19 Jan 2018 gcctggagag cccaggctgt gaccagaggc tcaactcctc aagtgggcag agctgacgca 660 tacttacaaa tggcttcaag agcaacgaga tcaggcatag aaggagtctt ccctaatgtt 720 tggcctataa acgtattcga accatgctgg tcactgtaca ctctccatct tgccggtctg 780 ttcgcccatc cagcactggc tgaggctgta agagttatcg ttgctcaact tgaagcaaga 840 ttgggagtgc atggcctcgg accagcttta cattttgctg ccgacgctga tgatactgca 900 gttgccttat gcgttctgca tttggctggc agagatcctg cagttgacgc attgagacat 960 tttgaaattg gtgagctctt tgttacattc ccaggagaga gaaatgctag tgtctctacg 1020 aacattcacg ctcttcatgc tttgagattg ttaggtaaac cagctgccgg agcaagtgca 1080 tacgtcgaag caaatagaaa tccacatggt ttgtgggaca acgaaaaatg gcacgtttca 1140 tggctttatc caactgcaca cgccgttgca gctctagctc aaggcaagcc tcaatggaga 1200 gatgaaagag cactagccgc tctactacaa gctcaaagag atgatggtgg ttggggagct 1260 ggtagaggat ccactttcga ggaaaccgcc tacgctcttt tcgctttaca cgttatggac 1320 ggatctgagg aagccacagg cagaagaaga atcgctcaag tcgtcgcaag agccttagaa 1380 tggatgctag ctagacatgc cgcacatgga ttaccacaaa caccactctg gattggtaag 1440 gaattgtact gtcctactag agtcgtaaga gtagctgagc tagctggcct gtggttagca 1500 ttaagatggg gtagaagagt attagctgaa ggtgctggtg ctgcacctta a 1551 <210> 37 <211> 2364 <212> DNA <213> Stevia rebaudiana <400> 37 atgaagaccg gcttcatctc tcccgccacc gtcttccacc accgtatttc tccggcaacc 60 accttccgcc accacctttc tccggcgacc accaactcca ctggaattgt agctcttaga 120 gacatcaact tccggtgtaa agcggtatcc aaagagtact ctgatttact acaaaaagat 180 gaggcttcat ttaccaagtg ggacgatgac aaagtgaagg accatttgga cacaaataag 240 aatttgtatc caaacgatga gatcaaggag tttgttgaga gcgtgaaagc aatgtttggt 300 tctatgaatg acggagaaat aaatgtgtca gcgtatgata cggcttgggt tgcactcgtg 360 caagatgttg atggaagtgg ttcccctcaa tttccatcaa gtttggagtg gatcgcgaac 420 aatcaactct cagatgggtc ttggggcgat catttgttat tttcggctca tgataggatc 480 attaacacgt tggcatgtgt tatagcgctt acttcttgga acgtccatcc aagtaaatgt 540 gaaaaaggac tgaattttct tagagaaaac atatgtaaac tcgaagacga gaacgcggaa 600 catatgccaa ttggttttga agtcacgttc ccgtcgctaa tagatatcgc aaagaagcta 660 aatattgaag ttcctgagga tactcctgcc ttaaaagaaa tttatgcaag aagagacata 720 aaactcacaa agataccaat ggaagtattg cacaaagtgc ccacaacttt acttcatagt 780 ttggaaggaa tgccagattt ggaatgggaa aaacttctga aattgcaatg caaagatgga 840 tcatttctgt tttctccatc atctactgct tttgcactca tgcaaacaaa agatgaaaag 900

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2018200459 19 Jan 2018

tgtcttcagt atttgacaaa tattgttacc aaattcaatg gtggagttcc gaatgtgtac 960 ccggtggatc tattcgaaca tatttgggta gttgatcgac ttcaacgact tgggattgct 1020 cgttatttca aatcagagat caaagattgc gttgaatata ttaacaagta ttggacaaag 1080 aatgggattt gttgggcaag aaacacgcac gtacaagata ttgatgatac cgcaatggga 1140 tttagggttt taagagcaca tggttatgat gttactccag atgtatttcg acaatttgag 1200 aaggatggta aattcgtatg tttcgctgga cagtcaacac aagccgtcac cggaatgttc 1260 aatgtgtata gagcgtcaca aatgctcttt cccggagaaa gaattcttga agatgcaaag 1320 aaattttcat ataattattt gaaagaaaaa caatcgacaa atgagcttct tgataaatgg 1380 atcatcgcca aagacttacc tggagaggtt ggatatgcgc tagacatacc atggtatgca 1440 agcttaccgc gactcgagac aagatattac ttagagcaat acgggggcga ggatgatgtt 1500 tggattggaa aaactctata caggatggga tatgtgagca ataatacgta ccttgaaatg 1560 gccaaattgg actacaataa ctatgtggcc gtgcttcaac tcgaatggta cactatccag 1620 caatggtatg ttgatatcgg tatcgaaaag tttgaaagtg acaatatcaa aagcgtatta 1680 gtgtcgtatt acttggctgc agccagcata ttcgagccgg aaaggtccaa ggaacgaatc 1740 gcgtgggcta aaaccaccat attagttgac aagatcacct caatttttga ttcatcacaa 1800 tcctcaaaag aggacataac agcctttata gacaaattta ggaacaaatc gtcttctaag 1860 aagcattcaa taaatggaga accatggcac gaggtgatgg ttgcactgaa aaagacccta 1920 cacggcttcg ctttggatgc actcatgact catagtcaag acatccaccc gcaactccat 1980 caagcttggg agatgtggtt gacgaaattg caagatggag tagatgtgac agcggaatta 2040 atggtacaaa tgataaatat gacagctggt cgttgggtat ccaaagaact tttaactcat 2100 cctcaatacc aacgcctctc aaccgtcaca aatagtgtgt gtcacgatat aactaagctc 2160 cataacttca aggagaattc cacgacggta gactcgaaag ttcaagaact agtgcaactt 2220 gtgtttagcg acacgcccga tgatcttgat caggatatga aacagacgtt tctaaccgtc 2280 atgaaaacct tctactacaa ggcgtggtgt gatccgaaca cgataaatga ccatatctcc 2340 aaggtgttcg agattgtaat atga 2364

<210> 38 <211> 1584 <212> DNA <213> Streptomyces clavuligerus

<400> 38 ttgcccgacg cgcatgatgc ccctccgcct cagatacgac agcggaccct tgtcgatgag 60 gcgacgcaac tcctcacgga gtcggccgag gacgcctggg gtgaggtgtc cgtgtccgag 120 tacgaaacgg cgcggctggt ggcccacgcc acctggctcg gcggtcacgc cacacgggtg 180 gccttcctgc tggagcggca gcatgaggac ggctcgtggg gcccgcccgg cgggtaccgt 240 ctcgtaccca cgctgagtgc cgtacacgcc ctgctcacct gtctggcgtc tcccgcgcag 300 gaccacggag tgcctcatga ccggctcctg cgcgcagttg acgcgggcct gacggcactg 360

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2018200459 19 Jan 2018

cgtcgtcttg ggacgagcga cagcccgccg gacaccattg cggtcgaact ggtcataccc 420 tcgctccttg agggcatcca gcacctcctg gacccggcgc acccgcattc ccgacccgct 480 ttttcgcaac accgcggcag cctcgtctgc cccgggggcc tcgacggccg cacgctgggg 540 gccttgcgct cccacgccgc agccggcaca cctgtcccgg gcaaggtgtg gcacgcctcg 600 gaaaccttgg ggctatcgac cgaggcagcc tcccaccttc aacccgccca gggcatcatc 660 ggtggctccg ccgccgcgac agcaacatgg ctcaccaggg tcgccccgtc gcaacagagc 720 gacagcgcac ggcgctacct ggaagaactc cagcaccgat acagcggccc ggtgccctcc 780 atcaccccga tcacctattt cgaacgggcc tggctgctca acaacttcgc tgccgcgggg 840 gttccatgcg aggctccggc agcccttctc gacagcctgg aggcagcgct cacaccacag 900 ggcgctccag cgggtgcggg actgccgccg gacgcggatg acaccgccgc cgttctgctg 960 gcgcttgcca cgcacggccg cgggcgccgt cccgaggtcc tcatggacta ccgcacggac 1020 ggctacttcc agtgcttcat cggcgaacgc accccttcca tcagcaccaa tgcccatgtc 1080 ctggagacgc tcggtcacca cgtcgcccaa caccctcagg acagggcccg atacggctca 1140 gccatggaca ccgcatcagc gtggctcctc gcggctcaga agcaggatgg cagctggctc 1200 gacaagtggc acgcctcccc ctactacgcc accgtctgct gcacccaggc actggcagcc 1260 cacgcttccc ctgccaccgc ccccgcacgg cagcgtgctg tgcggtgggt gctggcaaca 1320 caacgctcgg acggcggctg gggcctgtgg cactccacgg tcgaggagac cgcctacgcc 1380 ctgcagatcc tcgccccacc ttccggcggc gggaacatcc ccgtgcaaca ggcgctcacc 1440 agggggcgcg cccgcctctg cggcgctttg ccgctgactc ccctatggca tgacaaggac 1500 ctgtacacgc cggtacgtgt cgtccgcgcc gcccgtgccg ccgccctgta caccacccgt 1560 gacctgcttc tgccgcccct gtga 1584

<210> 39 <211> 1551 <212> DNA <213> Bradyrhizobium japonicum <400> 39 gtgaacgcgc tgtccgaaca tatcctttcc gaattgcgcc gcctgctgag cgaaatgagc 60 gatggcggca gcgtcggtcc gtccgtctac gacacggcgc aggcgctgcg cttccacggc 120 aacgtcaccg gtcggcagga cgcatacgcg tggctcatcg cgcagcaaca ggccgacggc 180 ggatggggaa gcgcggactt cccgctgttc cgccatgcgc ccacgtgggc ggcgttactg 240 gcattgcagc gtgccgatcc tcttcccgga gctgcggacg cagtccagac tgcaacgagg 300 ttcctccagc gccagcccga tccctacgca catgcggtgc cagaagacgc gccgatcggc 360 gcggagctga tcctgccgca gttttgcggt gaggccgcat ggttgctggg tggcgtagcg 420 tttccgcgcc atcctgcgct gttgccattg cggcaagcgt gcctggtcaa gctgggggcg 480 gtggcgatgt tgccgagcgg ccatccgttg ctacactcct gggaagcctg ggggacgtcg 540 ccgaccaccg catgcccgga tgacgacggc agcatcggca tcagtccggc ggccaccgcc 600

Page 38

2018200459 19 Jan 2018 gcgtggcgtg cccaggccgt gacacggggg agcacgccgc aggtcgggcg cgccgatgcg 660 tatctgcaga tggcatcgcg ggcgacgcgc agcggcatcg aaggtgtctt tcccaacgtc 720 tggccgatca atgtgttcga gccatgctgg tcgctgtaca ccctgcatct ggccgggctt 780 ttcgcgcatc ccgcgctcgc ggaggcggtt cgcgtgatcg tcgcgcagct cgaggcccgt 840 ctgggcgtgc acggtctggg cccggccttg cacttcgcgg ctgatgcgga cgacaccgcc 900 gttgcgttgt gcgtcctgca ccttgcaggc cgtgacccgg cggtcgatgc gttgcgccat 960 ttcgaaatcg gcgagctgtt cgtcaccttc cccggcgaac gcaatgcctc ggtgtcgacc 1020 aacattcatg ccctgcatgc gttgcgactg ttgggaaagc ccgccgcggg cgccagcgcg 1080 tacgtcgagg ccaatcgcaa cccgcacggt ctatgggaca acgaaaaatg gcacgtttcg 1140 tggctgtatc ccaccgcgca tgcggtcgct gcgctggcgc aaggcaagcc ccagtggcga 1200 gatgagcgcg cgctggcggc gctgctgcag gcgcagcgcg acgacggtgg ctggggcgcg 1260 ggtcgcgggt ccacgttcga ggaaaccgcc tatgcgctgt ttgcgttgca cgtgatggat 1320 gggagcgaag aggcgacagg gcgccggcgc atcgcgcagg tggtggcgcg tgcgctggag 1380 tggatgctcg cccgccatgc ggcgcatgga ttgccgcaga cgccgctgtg gatcggcaag 1440 gaactgtatt gccccactcg ggtcgtgcgc gtggccgaac tcgccgggtt gtggctggcg 1500 cttcgttggg ggcggcgcgt cctggccgag ggggcaggag cggcgccatg a 1551 <210> 40 <211> 2355 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 40

atgaatttga gtttgtgtat agcatctcca ctattgacca aatctaatag accagctgct 60 ttatcagcaa ttcatacagc tagtacatcc catggtggcc aaaccaaccc tacgaatctg 120 ataatcgata cgaccaagga gagaatacaa aaacaattca aaaatgttga aatttcagtt 180 tcttcttatg atactgcgtg ggttgccatg gttccatcac ctaattctcc aaagtctcca 240 tgtttcccag aatgtttgaa ttggctgatt aacaaccagt tgaatgatgg atcttggggt 300 ttagtcaatc acacgcacaa tcacaaccat ccacttttga aagattcttt atcctcaact 360 ttggcttgca tcgtggccct aaagagatgg aacgtaggtg aggatcagat taacaagggg 420 cttagtttca ttgaatctaa cttggcttcc gcgactgaaa aatctcaacc atctccaata 480 ggattcgata tcatctttcc aggtctgtta gagtacgcca aaaatctaga tatcaactta 540 ctgtctaagc aaactgattt ctcactaatg ttacacaaga gagaattaga acaaaagaga 600 tgtcattcaa acgaaatgga tggttaccta gcttatatct ctgaaggtct tggtaatctt 660 tacgattgga atatggtgaa aaagtaccag atgaaaaatg gctcagtttt caattcccct 720 tctgcaactg cggcagcatt cattaaccat caaaatccag gatgcctgaa ctatttgaat Page 39 780

2018200459 19 Jan 2018

tcactactag acaaattcgg caacgcagtt ccaactgtat accctcacga tttgtttatc 840 agattgagta tggtggatac aattgaaaga cttggtatat cccaccactt tagagtcgag 900 atcaaaaatg ttttggatga gacataccgt tgttgggtgg agagagatga acaaatcttt 960 atggatgttg tgacgtgcgc gttggccttt agattgttgc gtattaacgg ttacgaagtt 1020 agtccagatc cacttgccga aattacaaac gaattagctt taaaggatga atacgccgct 1080 cttgaaacat atcatgcgtc acatatcctt taccaagagg acttatcatc tggaaaacaa 1140 attcttaaat ctgctgattt cctgaaggaa atcatatcca ctgatagtaa tagactgtcc 1200 aaactgatcc ataaagaggt tgaaaatgca cttaagttcc ctattaacac cggcttagaa 1260 cgtattaaca caagacgtaa catccagctt tacaacgtag acaatactag aatcttgaaa 1320 accacttacc attcttccaa catatcaaac actgattacc taagattagc tgttgaagat 1380 ttctacacat gtcagtctat ctatagagaa gagctgaaag gattagagag atgggtcgtt 1440 gagaataagc tagatcaatt gaaatttgcc agacaaaaga cagcttattg ttacttctca 1500 gttgccgcca ctttatcaag tccagaattg tcagatgcac gtatttcttg ggctaaaaac 1560 ggaattttga caactgttgt tgatgatttc tttgatattg gcgggacaat cgacgaattg 1620 acaaacctga ttcaatgcgt tgaaaagtgg aatgtcgatg tcgataaaga ctgttgctca 1680 gaacatgtta gaatactgtt cttggctctg aaagatgcta tctgttggat cggggatgag 1740 gctttcaaat ggcaagctag agatgtgacg tctcacgtca ttcaaacctg gctagaactg 1800 atgaactcta tgttgagaga agcaatttgg actagagatg catacgttcc tacattaaac 1860 gagtatatgg aaaacgctta tgtctccttt gctttgggtc ctatcgttaa gcctgccata 1920 tactttgtag gaccaaagct atccgaggaa atcgtcgaat catcagaata ccataacttg 1980 ttcaagttaa tgtccacaca aggcagatta cttaatgata ttcattcttt caaaagagag 2040 tttaaggaag gaaagttaaa tgctgttgct ctgcatcttt ctaatggcga aagtggtaaa 2100 gtcgaagagg aagtagttga ggaaatgatg atgatgatca aaaacaagag aaaggagttg 2160 atgaaactaa tcttcgaaga gaacggttca attgttccta gagcatgtaa ggatgcattt 2220 tggaacatgt gtcatgtgct aaactttttc tacgcaaacg acgatggttt tactgggaac 2280 acaatactag atacagtaaa agacatcata tacaaccctt tggtcttagt aaacgaaaac 2340 gaggagcaaa gataa 2355

<210> 41 <211> 2355 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 41 atgaatctgt ccctttgtat agctagtcca ctgttgacaa aatcttctag accaactgct 60 ctttctgcaa ttcatactgc cagtactagt catggaggtc aaacaaaccc aacaaatttg 120

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2018200459 19 Jan 2018

ataatcgata ctactaagga gagaatccaa aagctattca aaaatgttga aatctcagta 180 tcatcttatg acaccgcatg ggttgcaatg gtgccatcac ctaattcccc aaaaagtcca 240 tgttttccag agtgcttgaa ttggttaatc aataatcagt taaacgatgg ttcttggggt 300 ttagtcaacc acactcataa ccacaatcat ccattattga aggactcttt atcatcaaca 360 ttagcctgta ttgttgcatt gaaaagatgg aatgtaggtg aagatcaaat caacaagggt 420 ttatcattca tagaatccaa tctagcttct gctaccgaca aatcacaacc atctccaatc 480 gggttcgaca taatcttccc tggtttgctg gagtatgcca aaaaccttga tatcaactta 540 ctgtctaaac aaacagattt ctctttgatg ctacacaaaa gagagttaga gcagaaaaga 600 tgccattcta acgaaattga cgggtactta gcatatatct cagaaggttt gggtaatttg 660 tatgactgga acatggtcaa aaagtatcag atgaaaaatg gatccgtatt caattctcct 720 tctgcaactg ccgcagcatt cattaatcat caaaaccctg ggtgtcttaa ctacttgaac 780 tcactattag ataagtttgg aaatgcagtt ccaacagtct atcctttgga cttgtacatc 840 agattatcta tggttgacac tatagagaga ttaggtattt ctcatcattt cagagttgag 900 atcaaaaatg ttttggacga gacatacaga tgttgggtcg aaagagatga gcaaatcttt 960 atggatgtcg tgacctgcgc tctggctttt agattgctaa ggatacacgg atacaaagta 1020 tctcctgatc aactggctga gattacaaac gaactggctt tcaaagacga atacgccgca 1080 ttagaaacat accatgcatc ccaaatactt taccaggaag acctaagttc aggaaaacaa 1140 atcttgaagt ctgcagattt cctgaaaggc attctgtcta cagatagtaa taggttgtct 1200 aaattgatac acaaggaagt agaaaacgca ctaaagtttc ctattaacac tggtttagag 1260 agaatcaata ctaggagaaa cattcagctg tacaacgtag ataatacaag gattcttaag 1320 accacctacc atagttcaaa catttccaac acctattact taagattagc tgtcgaagac 1380 ttttacactt gtcaatcaat ctacagagag gagttaaagg gcctagaaag atgggtagtt 1440 caaaacaagt tggatcaact gaagtttgct agacagaaga cagcatactg ttatttctct 1500 gttgctgcta ccctttcatc cccagaattg tctgatgcca gaataagttg ggccaaaaat 1560 ggtattctta caactgtagt cgatgatttc tttgatattg gaggtactat tgatgaactg 1620 acaaatctta ttcaatgtgt tgaaaagtgg aacgtggatg tagataagga ttgctgcagt 1680 gaacatgtga gaatactttt cctggctcta aaagatgcaa tatgttggat tggcgacgag 1740 gccttcaagt ggcaagctag agatgttaca tctcatgtca tccaaacttg gcttgaactg 1800 atgaactcaa tgctaagaga agcaatctgg acaagagatg catacgttcc aacattgaac 1860 gaatacatgg aaaacgctta cgtctcattt gccttgggtc ctattgttaa gccagccata 1920 tactttgttg ggccaaagtt atccgaagag attgttgagt cttccgaata tcataaccta 1980 ttcaagttaa tgtcaacaca aggcagactt ctgaacgata tccactcctt caaaagagaa 2040 ttcaaggaag gtaagctaaa cgctgttgct ttgcacttgt ctaatggtga atctggcaaa 2100 gtggaagagg aagtcgttga ggaaatgatg atgatgatca aaaacaagag aaaggaattg 2160

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2018200459 19 Jan 2018

atgaaattga ttttcgagga aaatggttca atcgtaccta gagcttgtaa agatgctttt 2220 tggaatatgt gccatgttct taacttcttt tacgctaatg atgatggctt cactggaaat 2280 acaatattgg atacagttaa agatatcatc tacaacccac ttgttttggt caatgagaac 2340 gaggaacaaa gataa 2355

<210> 42 <211> 1773 <212> DNA <213> Artificial Sequence

<220> <223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 42 atggctatgc cagtgaagct aacacctgcg tcattatcct taaaagctgt gtgctgcaga 60 ttctcatccg gtggccatgc tttgagattc gggagtagtc tgccatgttg gagaaggacc 120 cctacccaaa gatctacttc ttcctctact actagaccag ctgccgaagt gtcatcaggt 180 aagagtaaac aacatgatca ggaagctagt gaagcgacta tcagacaaca attacaactt 240 gtggatgtcc tggagaatat gggaatatcc agacattttg ctgcagagat aaagtgcata 300 ctagacagaa cttacagatc ttggttacaa agacacgagg aaatcatgct ggacactatg 360 acatgtgcta tggcttttag aatcctaaga ttgaacggat acaacgtttc atcagatgaa 420 ctataccacg ttgtagaggc atctggtctg cataattctt tgggtgggta tcttaacgat 480 accagaacac tacttgaatt acacaaggct tcaacagtta gtatctctga ggatgaatct 540 atcttagatt caattggctc tagatccaga acattgctta gagaacaatt ggagtctggt 600 ggcgcactga gaaagccttc tttattcaaa gaggttgaac atgcactgga tggacctttt 660 tacaccacac ttgatagact tcatcatagg tggaatattg aaaacttcaa cattattgag 720 caacacatgt tggagactcc atacttatct aaccagcata catcaaggga tatcctagca 780 ttgtcaatta gagatttttc ctcctcacaa ttcacttatc aacaagagct acagcatctg 840 gagagttggg ttaaggaatg tagattagat caactacagt tcgcaagaca gaaattagcg 900 tacttttacc tatcagccgc aggcaccatg ttttctcctg agctttctga tgcgagaaca 960 ttatgggcca aaaacggggt gttgacaact attgttgatg atttctttga tgttgccggt 1020 tctaaagagg aattggaaaa cttagtcatg ctggtcgaaa tgtgggatga acatcacaaa 1080 gttgaattct attctgagca ggtcgaaatc atcttctctt ccatctacga ttctgtcaac 1140 caattgggtg agaaggcctc tttggttcaa gacagatcaa ttacaaaaca ccttgttgaa 1200 atatggttag acttgttaaa gtccatgatg acggaagttg aatggagact gtcaaaatac 1260 gtgcctacag aaaaggaata catgattaat gcctctctta tcttcggcct aggtccaatc 1320 gttttaccag ctttgtattt cgttggtcca aagatttcag aaagtatagt aaaggaccca 1380 gaatatgatg aattgttcaa actaatgtca acatgtggta gattgttgaa tgacgtgcaa 1440 acgttcgaaa gagaatacaa tgagggtaaa ctgaattctg tcagtctatt ggttcttcac 1500 Page 42

2018200459 19 Jan 2018 ggaggcccaa agaagagatc gaactattct tctagtcaag caaggttctc <210>

<211>

<212>

<213>

<220>

<223>

<400> 43 atgcagaact tccgtttctt acaccttgtt tggtcacttc tgtattctgg ttcatagaac gacattatct aaaccaactg ggcaaaaatc ctgcaagatt ccatcaacaa cgttctcttc gccagacttt gagagaaagt ttctccgata gtctctcttg gctttagaac caaaattcta gacagattgc gctaacttac aggattctaa gcagtggaag agatgggtcg tgctatttct tgtctatttc ttctttcttt ggaaaatgtg tcgaaagagc atacactggt

2232

DNA

Artificial Sequence

Description of Artificial polynucleotide tccatggtac cttatgatac ttccagaatg ctcatggcaa ctcttaaaag tcaactctgc ttccaggtat acattaactc tagaaggtag gggaaatggc ctgcagctgc tccagaaatt caatggtaga tcgttctgga acgcaacctg aagatcactt tgtacagagc gaacttctta gtaaaaacat aaagattagc aaacttccta atttcaatat ttgaaagacg cagccgcagc agacgcaaag ggtccttaga taaagtgtgc aaaagaggta atctgatgtt aaaggaaagg agcctgggtt tactaaatgg tccacttcta atggggaatc tagtgtaacc gattgaatac catgttgcat aagagcttac tatgaaatac attcatccat tggaaacgca tgccctggaa tgaaacatac tgctttggcc ctctaactct cctccaattg cttcttaaaa aattggagag tattcgtaga cagatgctca ctgtcaatca tctagacaag aacattgttt aggaaattac gaagagtctg tatttctttt gacgctgtca taa

Sequence:

atcaaaaaga gcaatggtcc atcctagaaa gttaaagatg ggtgaggaac gataacgaac gctatagact cgtagagccc ttggcctacg caacgtaaaa atacaagatg gtccctacaa cgtcttggta agattttggt ttcagaatat ctgggcggtt tcttacccag caaggtttat gtgcatgatg aggattaagc acaatcggta atacaaagag ttaaagttcg gcccctgaat aaaagcctat tagtaccaag actcaacaac taaatgagcc

Syntheti c tgtttgacaa catcccctga atcagttggg cattatcttc agattaacaa aacacaaacc tagacctgaa ttgaattgac tctctgaagg acggatctct ctgaatgcct tataccctct ttgatagaca tgcaaggaga tgagacttaa acttaaagga acgagtccct ccaatgtctc ctttaaactt attacgctac accaagattt aggaattcaa ctagacaaaa tgtctgatgc tgatacgtgt accatgtaag tgatgggttt actgaagttg

1560

1620

1680

1740

1773 gattgaattg ttgcccagaa tgatggtagt cactcttgct aggactgaga aattggattt tctaccacta atcaggtgga aatcggtaag gttcaatagt ccactatatt cgatatctat tttcagaaag agaggagatt tggttacgat ctcaggagca cctggaaaag cctctgtggt ttccgaccac tgacgataca tctaaaactt gcatattgaa agaggcctat tagaatgtct

120

180

240

300

360

420

480

540

600

660

720

780

840

900

960

1020

1080

1140

1200

1260

1320

1380

1440

Page 43

2018200459 19 Jan 2018

tgggccaaaa atggtgtatt gacaactgtg gttgatgatt tcttcgatgt cggaggctct 1500 gaagaggaat tagttaactt gatagaattg atcgagcgtt gggatgtgaa tggcagtgca 1560 gatttttgta gtgaggaagt tgagattatc tattctgcta tccactcaac tatctctgaa 1620 ataggtgata agtcatttgg ctggcaaggt agagatgtaa agtctcaagt tatcaagatc 1680 tggctggact tattgaaatc aatgttaact gaagctcaat ggtcttcaaa caagtctgtt 1740 cctaccctag atgagtatat gacaaccgcc catgtttcat tcgcacttgg tccaattgta 1800 cttccagcct tatacttcgt tggcccaaag ttgtcagaag aggttgcagg tcatcctgaa 1860 ctactaaacc tctacaaagt cacatctact tgtggcagac tactgaatga ttggagaagt 1920 tttaagagag aatccgagga aggtaagctc aacgctatta gtttatacat gatccactcc 1980 ggtggtgctt ctacagaaga ggaaacaatc gaacatttca aaggtttgat tgattctcag 2040 agaaggcaac tgttacaatt ggtgttgcaa gagaaggata gtatcatacc tagaccatgt 2100 aaagatctat tttggaatat gattaagtta ttacacactt tctacatgaa agatgatggc 2160 ttcacctcaa atgagatgag gaatgtagtt aaggcaatca ttaacgaacc aatctcactg 2220 gatgaattat ga 2232

<210> 44 <211> 2355 <212> DNA <213> Stevia rebaudiana

<400> 44 atgaatcttt cactatgcat cgcgtcccct ttgttaacca aatcaaatcg acccgcggct 60 ctgtcagcta ttcatacagc atcaacttca catggtggac aaactaatcc cactaatctg 120 atcattgata caaccaaaga acggatccaa aaacagttta aaaatgtaga aatttctgtt 180 tcttcatatg acacagcatg ggtagccatg gtcccttctc caaactcacc caaatcgcct 240 tgtttccctg agtgtctcaa ttggttaatt aataatcagc ttaatgatgg ttcatggggt 300 cttgttaatc acactcataa tcataatcac ccgttgctta aagattctct atcttcaaca 360 ttagcatgta ttgttgcatt aaaaagatgg aatgttgggg aagatcaaat aaataaaggt 420 ctaagtttta ttgagtcaaa tcttgcttca gctactgaaa aaagtcaacc atctcccatt 480 ggttttgaca tcatatttcc tggtttgctt gagtatgcga aaaacttgga cataaacctc 540 ctttcaaaac aaacagattt tagtttgatg ctacataaga gggaattgga gcaaaaaaga 600 tgccattcaa atgagatgga tggatacttg gcgtatatct ctgaaggact cggtaattta 660 tatgattgga atatggtgaa gaaatatcag atgaaaaatg gttctgtttt caactcacca 720 tcagcaacag ctgctgcttt cattaatcat caaaatcctg gttgtcttaa ttatttaaat 780 tcacttttgg acaagtttgg taatgcagtc ccaacagttt atcctcatga tttatttatc 840 cgactttcta tggttgacac aattgaaaga ttaggaattt cacaccattt cagagtggaa 900 attaaaaatg ttttagatga aacatacaga tgttgggtgg aacgagatga gcaaatattc 960 atggatgttg taacatgtgc tttagccttt cggttattaa ggatcaatgg gtatgaagtt 1020

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2018200459 19 Jan 2018

tccccagatc cattggctga aattactaat gaattagctt tgaaagacga atatgcagct 1080 cttgaaacat atcatgcgtc acatatatta taccaagagg atttatcttc tggaaaacaa 1140 atcttgaagt cagctgattt cctcaaagag ataatatcca ctgattcaaa caggctttct 1200 aaattaattc acaaagaggt ggaaaatgct cttaagttcc ctatcaatac cggtttagaa 1260 cgcataaaca ctagacgaaa tatacagctt tacaatgtag acaatacaag aattctgaaa 1320 actacatatc actcatcaaa tattagtaac actgattacc taaggttggc tgttgaagat 1380 ttctacacct gccaatctat ttatcgtgaa gaattaaaag gtcttgaaag gtgggtggta 1440 gagaataagt tggaccagct caagtttgct aggcaaaaga ccgcctactg ttatttctct 1500 gttgctgcaa cactttcgtc tcccgaatta tcagatgcgc gtatttcatg ggccaaaaat 1560 ggcatattaa ctacagtagt tgatgacttt tttgatatcg gtggtacaat cgatgaattg 1620 accaacctga ttcaatgtgt tgaaaaatgg aatgtagatg tcgacaagga ttgttgttca 1680 gagcatgttc ggattttatt tttagcatta aaagatgcaa tctgttggat tggagatgaa 1740 gcttttaaat ggcaagcgcg cgatgtaact agccatgtta ttcaaacttg gttggaacta 1800 atgaatagta tgttgagaga agctatatgg acaagagatg cttatgtgcc aacattaaat 1860 gaatatatgg aaaacgctta cgtgtcattt gcattaggcc cgattgtcaa gccggctatt 1920 tactttgtgg ggcccaaatt atcagaggag attgttgaaa gctctgaata tcataatcta 1980 tttaagctaa tgagcacgca gggtcgactt ctaaacgata tccatagctt caagagggaa 2040 tttaaggaag gcaaattaaa cgcggtagca ttgcatttga gtaacggaga aagtgggaaa 2100 gtggaagaag aggttgtgga ggagatgatg atgatgatta aaaacaagag gaaagaatta 2160 atgaaattaa tttttgaaga aaatggtagc attgttccta gagcttgtaa agatgcattt 2220 tggaacatgt gtcacgtgtt gaattttttt tacgcaaacg atgacgggtt tactggaaac 2280 acgattcttg atactgtgaa ggacatcatt tacaacccgt tggtgcttgt gaatgaaaat 2340 gaagaacaaa ggtaa 2355

<210> 45 <211> 2355 <212> DNA <213> Stevia rebaudiana

<400> 45 atgaatcttt cactatgcat tgcgtcccct ttgttaacca aatcaagtcg acccacggct 60 ctgtcagcta ttcatacagc atcaacttca catggtggac aaactaatcc cactaatctg 120 atcattgata caaccaaaga acggatccaa aaactgttta aaaatgtaga aatttctgtt 180 tcttcatatg acacagcatg ggtagccatg gtcccttctc caaactcacc caaatcgcct 240 tgtttccctg agtgtctcaa ttggttaatt aataatcagc ttaatgatgg ttcatggggt 300 cttgttaatc acactcataa tcataatcac ccgttgctta aagattctct atcttcaaca 360 ttagcatgta ttgttgcatt aaaaagatgg aatgttgggg aagatcaaat aaataaaggt 420 ctaagtttta ttgagtcaaa tcttgcttca gcaactgaca aaagtcaacc atctcccatt 480

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2018200459 19 Jan 2018

ggttttgata tcatatttcc tggtttgctt gagtatgcga aaaacttgga cataaacctc 540 ctttcaaaac aaacagattt tagtttgatg ctacataaga gggaattgga gcaaaaaaga 600 tgccattcaa atgagattga tggatacttg gcgtatatct ctgaaggact cggtaattta 660 tatgattgga atatggtgaa gaaatatcag atgaaaaatg gttctgtttt caactcacca 720 tcagcaacag cagctgcttt cattaatcat caaaatcccg gttgtcttaa ttatttaaat 780 tcacttttgg acaagtttgg taatgcagtc ccaacagttt atcctcttga tttatatatc 840 cggctttcta tggttgacac aattgaaaga ttaggaattt cacaccattt cagagtggaa 900 attaaaaatg ttttagatga aacatacaga tgttgggtgg aacgagatga gcaaatattc 960 atggatgttg taacatgtgc tttagccttt cggttattaa ggatccacgg gtataaagtc 1020 tccccagatc aattggctga aattactaat gaattagctt tcaaagacga atacgcagct 1080 cttgaaacat atcatgcatc acagatatta taccaagagg atttatcttc tggaaaacaa 1140 atcttgaagt cagctgattt cctcaaaggg atattatcca ctgattcaaa caggctttct 1200 aaattaattc acaaagaggt ggaaaatgct cttaagttcc ctatcaatac cggtttagaa 1260 cgcataaaca ctagacgaaa tatacagctt tacaatgtag acaatacaag aattctgaaa 1320 actacatatc actcatcaaa tattagtaac acttattacc taaggttggc tgttgaagat 1380 ttctacacct gccaatctat ttatcgtgaa gaattaaaag gtcttgaaag gtgggtggta 1440 cagaataagt tggaccagct caagtttgct aggcaaaaga ccgcctactg ttatttctct 1500 gttgctgcaa cactttcgtc tcccgaatta tcagatgcgc gtatttcatg ggccaaaaat 1560 ggcatattaa ctacagtagt tgatgacttt tttgatatcg gtggtacaat cgatgaattg 1620 accaacctga ttcaatgtgt tgaaaaatgg aatgtagatg tcgacaagga ttgttgttca 1680 gagcatgttc ggattttatt tttagcatta aaagatgcaa tctgttggat tggagatgaa 1740 gcttttaaat ggcaagcgcg cgatgtaact agccatgtta ttcaaacttg gttggaacta 1800 atgaatagta tgttgagaga agctatatgg acaagagatg cttatgtgcc aacattaaat 1860 gaatatatgg aaaacgctta cgtgtcattt gcattaggcc cgattgtcaa gccggctatt 1920 tactttgtgg ggcccaaatt atcagaggag attgttgaaa gctctgaata tcataatcta 1980 tttaagctaa tgagcacgca gggtcgactt ctaaacgata tccatagctt caagagggaa 2040 tttaaggaag gcaaattaaa cgcggtagca ttgcatttga gtaacggaga aagtgggaaa 2100 gtggaagaag aggttgtgga ggagatgatg atgatgatta aaaacaagag gaaagaatta 2160 atgaaattaa tttttgaaga aaatggtagc attgttccta gagcttgtaa agatgcattt 2220 tggaacatgt gtcacgtgtt gaattttttt tacgcaaacg atgacgggtt tactggaaac 2280 acgattcttg atactgtgaa ggacatcatt tacaacccgt tggtgcttgt gaatgaaaat 2340 gaagaacaaa ggtaa 2355

<210> 46 <211> 1773 <212> DNA <213> Zea mays

Page 46

2018200459 19 Jan 2018 <400> 46

atggccatgc cagtgaagct gactcctgcc tccctctcgc tgaaggcggt ctgctgccgc 60 ttcagctccg gagggcatgc gctgcgcttc ggctcgtcgc taccgtgctg gaggaggacg 120 ccgacgcaac ggagcacgtc gtcgtctacg acgcgccctg cggctgaggt tagctctggc 180 aaaagcaagc agcacgatca agaagcatcg gaggctacga taagacagca gctccagcta 240 gtcgatgtgc ttgagaacat ggggatttct cggcattttg ctgctgaaat caaatgcatc 300 cttgacagga catacagaag ttggttacag agacatgagg aaattatgct ggacacaatg 360 acctgtgcga tggcatttcg tattctaagg ttgaatggat acaatgtctc ttctgatgag 420 ttgtatcatg ttgttgaagc ttccggactc cataattcac ttggaggata tctcaatgat 480 acaagaacct tgttagaatt acacaaggcc tcgacagtta gtatctctga agatgagtct 540 atcctggata gcataggctc aaggtcacgt accttactga gggaacaact agagtctggt 600 ggtgctctac gaaaaccttc actctttaaa gaggtggaac atgctctgga cggtcccttc 660 tacaccacat tggaccgtct acaccatagg tggaacatcg aaaatttcaa tattatagag 720 cagcacatgc tagagacacc atacttgtca aatcaacata ccagtagaga tattctagcg 780 ttgagtatta gagacttcag ttcctctcag tttacttacc agcaagaact tcaacatctt 840 gaaagctggg tgaaagagtg caggttagac cagctacaat ttgcgcgaca gaagttggca 900 tacttctact tgtctgctgc tggcaccatg ttctctcctg agctgtctga tgctcgaact 960 ttgtgggcca aaaatggtgt gctcacaact attgttgacg acttctttga tgttgcggga 1020 tcaaaagaag aacttgaaaa ccttgtcatg ttggttgaga tgtgggacga gcatcacaaa 1080 gttgagttct actcagaaca agtagagatt atattttctt caatttatga ctcagttaac 1140 caacttggtg aaaaggcttc tttggtacaa gaccgcagta ttaccaaaca cctagtagaa 1200 atatggttgg atttgctaaa gtctatgatg acagaggtag agtggcgttt gagcaaatat 1260 gtgccaacag agaaggaata catgataaat gcatctttaa tatttggact aggccccatt 1320 gtattgccag cattatattt tgttgggcca aagatctcag agtctattgt taaagatcca 1380 gaatatgatg aattgttcaa actgatgagc acatgtggtc gcctcttgaa tgatgttcag 1440 acttttgaga gggagtacaa cgagggcaag ttgaatagtg tttctctcct cgttcttcat 1500 ggtggcccca tgtccatatc agacgccaaa aggaaattac agaagcccat agacacatgc 1560 agaagagacc tcctaagttt agttcttcgt gaagaaagtg ttgttcctag gccctgcaag 1620 gaattatttt ggaaaatgtg caaggtgtgc tacttcttct actcgacgac ggatgggttt 1680 agctcacaag tggagagggc taaagaagtg gatgcggtga tcaatgagcc actaaagcta 1740 caaggaagtc atacgctggt gtctgatgtg tga 1773

<210> 47 <211> 2232 <212> DNA <213> Populus trichocarpa <400> 47

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2018200459 19 Jan 2018

atgcagaact ttcatggaac taaggaaagg atcaagaaga tgtttgataa gattgaattg 60 tcagtgtctt catatgacac tgcttgggtg gcaatggtcc catctccaga ttgtccggaa 120 actccttgtt ttccagagtg cacaaaatgg attttggaaa atcaacttgg tgatggctcc 180 tggagtcttc ctcatggcaa tccattatta gttaaggatg ctctttcatc tacattagcg 240 tgcatccttg cattgaagcg atggggtatc ggtgaagaac aaataaataa aggccttcga 300 tttattgagt tgaattccgc ttcagttacg gataacgagc aacataaacc aattggattt 360 gatataatat ttcctggcat gattgaatat gccatagatt tggatttgaa cctccctttg 420 aagccgacag atataaattc catgctccac aggagggctt tggagcttac aagtggcggt 480 ggcaagaact tggagggaag aagagcctac ttagcatatg tttcggaagg aattggaaaa 540 ttacaggatt gggaaatggc catgaaatat caaagaaaga atggatcact gttcaattca 600 ccatccacca cagcagctgc ctttattcat attcaagatg ctgagtgtct ccattatatt 660 cgttcactct tacagaagtt tgggaatgca gttccaacca tttatccttt ggatatatat 720 gctcgtcttt ctatggttga tgctcttgaa aggttgggaa tcgatcggca ttttaggaag 780 gaaagaaaat ttgttttgga cgaaacatac cgattttggt tgcaggggga ggaagagata 840 ttttctgata atgccacttg tgctttggca tttaggatat tacgtttgaa cggatatgat 900 gtctctctag aagatcattt ctctaattca ctgggaggat atttgaagga ttcgggagct 960 gccttagagt tgtacagagc tctgcagcta agttatccag atgaatcact tctggaaaaa 1020 caaaattctc ggacaagcta tttcctgaaa cagggattat ccaacgtttc actttgtgga 1080 gataggcttc gtaaaaatat tatcggagag gtgcatgatg ctctcaattt ttctgaccat 1140 gcaaatttgc aacgcttagc tatcagaaga agaattaaac attatgctac agatgatacg 1200 aggattttga aaacttcgta tcgttgttcg actattggta accaggattt tctcaaattg 1260 gctgtagaag acttcaatat ctgtcaatca atacagcgtg aagaatttaa acatatcgag 1320 aggtgggttg tagagaggag actggacaag ctaaagtttg ctaggcagaa ggaggcctac 1380 tgttacttct ctgctgcagc aactctcttc gctccagaac tatctgatgc acgcatgtca 1440 tgggcaaaaa atggtgtgct tactactgtt gttgatgact tctttgatgt tggtggttct 1500 gaagaagaac tggtaaacct tattgaattg attgagaggt gggatgtcaa tggcagtgct 1560 gatttttgtt ctgaggaagt tgagatcata tattcggcaa ttcacagcac tataagtgag 1620 ataggagaca aatctttcgg atggcaagga cgcgatgtga aaagtcaggt tatcaagatt 1680 tggttggatt tgctcaaatc catgttgaca gaagcacaat ggtcaagtaa caaatcagtg 1740 ccgacccttg atgaatatat gacaactgca catgtatcgt tcgctctagg gcctattgtt 1800 cttccagctc tgtattttgt ggggcctaag ctttcagagg aggttgctgg acatcctgaa 1860 ttgcttaatc tatacaaggt tacgagcact tgcgggcgtc tgctcaatga ctggagaagc 1920 tttaagagag aatctgaaga agggaaattg aatgccatct cattgtacat gattcacagc 1980 ggtggtgctt caactgaaga agagaccatc gaacatttta aaggattgat cgacagccag 2040 agaagacaat tgcttcaatt agttttgcag gaaaaggata gtataattcc tagaccctgc 2100

Page 48 aaggatttgt tttggaacat gataaaatta ttgcacacgt tctacatgaa ggatgatgga 2160 ttcacttcaa acgagatgag aaatgttgtc aaggcaataa taaatgaacc catctctcta 2220 gatgaattat aa 2232 <210> 48 <211> 2952 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

2018200459 19 Jan 2018

<400> 48 atggaatttg atgaaccatt ggttgacgaa gcaagatctt tagtgcagcg tactttacaa 60 gattatgatg acagatacgg cttcggtact atgtcatgtg ctgcttatga tacagcctgg 120 gtgtctttag ttacaaaaac agtcgatggg agaaaacaat ggcttttccc agagtgtttt 180 gaatttctac tagaaacaca atctgatgcc ggaggatggg aaatcgggaa ttcagcacca 240 atcgacggta tattgaatac agctgcatcc ttacttgctc taaaacgtca cgttcaaact 300 gagcaaatca tccaacctca acatgaccat aaggatctag caggtagagc tgaacgtgcc 360 gctgcatctt tgagagcaca attggctgca ttggatgtgt ctacaactga acacgtcggt 420 tttgagataa ttgttcctgc aatgctagac ccattagaag ccgaagatcc atctctagtt 480 ttcgattttc cagctaggaa acctttgatg aagattcatg atgctaagat gagtagattc 540 aggccagaat acttgtatgg caaacaacca atgaccgcct tacattcatt agaggctttc 600 ataggcaaaa tcgacttcga taaggtaaga caccaccgta cccatgggtc tatgatgggt 660 tctccttcat ctaccgcagc ctacttaatg cacgcttcac aatgggatgg tgactcagag 720 gcttacctta gacacgtgat taaacacgca gcagggcagg gaactggtgc tgtaccatct 780 gctttcccat caacacattt tgagtcatct tggattctta ccacattgtt tagagctgga 840 ttttcagctt ctcatcttgc ctgtgatgag ttgaacaagt tggtcgagat acttgagggc 900 tcattcgaga aggaaggtgg ggcaatcggt tacgctccag ggtttcaagc agatgttgat 960 gatactgcta aaacaataag tacattagca gtccttggaa gagatgctac accaagacaa 1020 atgatcaagg tatttgaagc taatacacat tttagaacat accctggtga aagagatcct 1080 tctttgacag ctaattgtaa tgctctatca gccttactac accaaccaga tgcagcaatg 1140 tatggatctc aaattcaaaa gattaccaaa tttgtctgtg actattggtg gaagtctgat 1200 ggtaagatta aagataagtg gaacacttgc tacttgtacc catctgtctt attagttgag 1260 gttttggttg atcttgttag tttattggag cagggtaaat tgcctgatgt tttggatcaa 1320 gagcttcaat acagagtcgc catcacattg ttccaagcat gtttaaggcc attactagac 1380 caagatgccg aaggatcatg gaacaagtct atcgaagcca cagcctacgg catccttatc 1440 ctaactgaag ctaggagagt ttgtttcttc gacagattgt ctgagccatt gaatgaggca 1500 atccgtagag gtatcgcttt cgccgactct atgtctggaa Page 49 ctgaagctca gttgaactac 1560

2018200459 19 Jan 2018

atttggatcg aaaaggttag ttacgcacct gcattattga ctaaatccta tttgttagca 1620 gcaagatggg ctgctaagtc tcctttaggc gcttccgtag gctcttcttt gtggactcca 1680 ccaagagaag gattggataa gcatgtcaga ttattccatc aagctgagtt attcagatcc 1740 cttccagaat gggaattaag agcctccatg attgaagcag ctttgttcac accacttcta 1800 agagcacata gactagacgt tttccctaga caagatgtag gtgaagacaa atatcttgat 1860 gtagttccat tcttttggac tgccgctaac aacagagata gaacttacgc ttccactcta 1920 ttcctttacg atatgtgttt tatcgcaatg ttaaacttcc agttagacga attcatggag 1980 gccacagccg gtatcttatt cagagatcat atggatgatt tgaggcaatt gattcatgat 2040 cttttggcag agaaaacttc cccaaagagt tctggtagaa gtagtcaggg cacaaaagat 2100 gctgactcag gtatagagga agacgtgtca atgtccgatt cagcttcaga ttcccaggat 2160 agaagtccag aatacgactt ggttttcagt gcattgagta cctttacaaa acatgtcttg 2220 caacacccat ctatacaaag tgcctctgta tgggatagaa aactacttgc tagagagatg 2280 aaggcttact tacttgctca tatccaacaa gcagaagatt caactccatt gtctgaattg 2340 aaagatgtgc ctcaaaagac tgatgtaaca agagtttcta catctactac taccttcttt 2400 aactgggtta gaacaacttc cgcagaccat atatcctgcc catactcctt ccactttgta 2460 gcatgccatc taggcgcagc attgtcacct aaagggtcta acggtgattg ctatccttca 2520 gctggtgaga agttcttggc agctgcagtc tgcagacatt tggccaccat gtgtagaatg 2580 tacaacgatc ttggatcagc tgaacgtgat tctgatgaag gtaatttgaa ctccttggac 2640 ttccctgaat tcgccgattc cgcaggaaac ggagggatag aaattcagaa ggccgctcta 2700 ttaaggttag ctgagtttga gagagattca tacttagagg ccttccgtcg tttacaagat 2760 gaatccaata gagttcacgg tccagccggt ggtgatgaag ccagattgtc cagaaggaga 2820 atggcaatcc ttgaattctt cgcccagcag gtagatttgt acggtcaagt atacgtcatt 2880 agggatattt ccgctcgtat tcctaaaaac gaggttgaga aaaagagaaa attggatgat 2940 gctttcaatt ga 2952

<210> 49 <211> 2646 <212> DNA <213> Artificial Sequence

<220> <223> Description of Artificial ! polynucleoti de Sequence: Synthetic <400> 49 atggcttcta gtacacttat ccaaaacaga tcatgtggcg tcacatcatc tatgtcaagt 60 tttcaaatct tcagaggtca accactaaga tttcctggca ctagaacccc agctgcagtt 120 caatgcttga aaaagaggag atgccttagg ccaaccgaat ccgtactaga atcatctcct 180 ggctctggtt catatagaat agtaactggc ccttctggaa ttaaccctag ttctaacggg 240 cacttgcaag agggttcctt gactcacagg ttaccaatac caatggaaaa atctatcgat 300

Page 50

2018200459 19 Jan 2018

aacttccaat ctactctata tgtgtcagat atttggtctg aaacactaca gagaactgaa 360 tgtttgctac aagtaactga aaacgtccag atgaatgagt ggattgagga aattagaatg 420 tactttagaa atatgacttt aggtgaaatt tccatgtccc cttacgacac tgcttgggtg 480 gctagagttc cagcgttgga cggttctcat gggcctcaat tccacagatc tttgcaatgg 540 attatcgaca accaattacc agatggggac tggggcgaac cttctctttt cttgggttac 600 gatagagttt gtaatacttt agcctgtgtg attgcgttga aaacatgggg tgttggggca 660 caaaacgttg aaagaggaat tcagttccta caatctaaca tatacaagat ggaggaagat 720 gacgctaatc atatgccaat aggattcgaa atcgtattcc ctgctatgat ggaagatgcc 780 aaagcattag gtttggattt gccatacgat gctactattt tgcaacagat ttcagccgaa 840 agagagaaaa agatgaaaaa gatcccaatg gcaatggtgt acaaataccc aaccacttta 900 cttcactcct tagaaggctt gcatagagaa gttgattgga ataagttgtt acaattacaa 960 tctgaaaatg gtagttttct ttattcacct gcttcaaccg catgcgcctt aatgtacact 1020 aaggacgtta aatgttttga ttacttaaac cagttgttga tcaagttcga ccacgcatgc 1080 ccaaatgtat atccagtcga tctattcgaa agattatgga tggttgacag attgcagaga 1140 ttagggatct ccagatactt tgaaagagag attagagatt gtttacaata cgtctacaga 1200 tattggaaag attgtggaat cggatgggct tctaactctt ccgtacaaga tgttgatgat 1260 acagccatgg cgtttagact tttaaggact catggtttcg acgtaaagga agattgcttt 1320 agacagtttt tcaaggacgg agaattcttc tgcttcgcag gccaatcatc tcaagcagtt 1380 acaggcatgt ttaatctttc aagagccagt caaacattgt ttccaggaga atctttattg 1440 aaaaaggcta gaaccttctc tagaaacttc ttgagaacaa agcatgagaa caacgaatgt 1500 ttcgataaat ggatcattac taaagatttg gctggtgaag tcgagtataa cttgaccttc 1560 ccatggtatg cctctttgcc tagattagaa cataggacat acttagatca atatggaatc 1620 gatgatatct ggataggcaa atctttatac aaaatgcctg ctgttaccaa cgaagttttc 1680 ctaaagttgg caaaggcaga ctttaacatg tgtcaagctc tacacaaaaa ggaattggaa 1740 caagtgataa agtggaacgc gtcctgtcaa ttcagagatc ttgaattcgc cagacaaaaa 1800 tcagtagaat gctattttgc tggtgcagcc acaatgttcg aaccagaaat ggttcaagct 1860 agattagtct gggcaagatg ttgtgtattg acaactgtct tagacgatta ctttgaccac 1920 gggacacctg ttgaggaact tagagtgttt gttcaagctg tcagaacatg gaatccagag 1980 ttgatcaacg gtttgccaga gcaagctaaa atcttgttta tgggcttata caaaacagtt 2040 aacacaattg cagaggaagc attcatggca cagaaaagag acgtccatca tcatttgaaa 2100 cactattggg acaagttgat aacaagtgcc ctaaaggagg ccgaatgggc agagtcaggt 2160 tacgtcccaa catttgatga atacatggaa gtagctgaaa tttctgttgc tctagaacca 2220 attgtctgta gtaccttgtt ctttgcgggt catagactag atgaggatgt tctagatagt 2280 tacgattacc atctagttat gcatttggta aacagagtcg gtagaatctt gaatgatata 2340

Page 51

2018200459 19 Jan 2018 caaggcatga gaacatccat aattcaatgc aagagaatcc ttctcatccc gagtaa

agagggaggc ttcacaaggt aagatctcat cagttcaaat ctacatggag 2400 ctgttccatc tgaggccatg gcgatcgctc atcttcaaga gttagttgat 2460 agcaattgac atacgaagtt cttaggttca ctgcggttcc aaaaagttgt 2520 acttgaatat ggctaaaatc atgcatgcct tctacaagga tactgatgga 2580 ttactgcaat gacaggattc gtcaaaaagg ttcttttcga acctgtgcct 2640 2646

<210> 50 <211> 2952 <212> DNA <213> Phomopsis amygdali

<400> 50 atggagttcg atgaaccact tgtggacgag gcgaggtcct tggtccaaag aaccctgcaa 60 gattatgacg accgctatgg ctttggcact atgagctgtg cggcctatga cacagcatgg 120 gtatcgctgg tgactaaaac agtcgatggg cgtaaacaat ggttgttccc tgagtgcttc 180 gaatttctcc tagaaacgca gtccgatgct ggcggctggg aaatcggcaa cagcgcaccc 240 atcgatggga tccttaacac tgctgcttca ctgctggcat tgaagcgcca cgtccaaaca 300 gagcagatta ttcagccgca acacgaccat aaagacctgg ccgggcgtgc ggaaagagcg 360 gcggcgtctt tgcgagcaca gttggcggct ctggatgtgt cgacaacgga gcatgtgggc 420 ttcgaaatca tcgtcccggc catgctcgac cctctcgagg ccgaagaccc gtctttggtg 480 ttcgactttc cagcacgcaa accactgatg aagatccacg acgctaagat gtcgcgattc 540 cgaccagagt acctctacgg taaacagccg atgacggcat tgcattcgct cgaggccttt 600 atcgggaaaa tagacttcga caaagtacgg catcacagga cacacggttc gatgatgggg 660 tcgccctcgt cgacggctgc atacctgatg catgcttctc agtgggacgg cgactctgag 720 gcctatctac gccatgtcat caagcacgca gctggccagg gcaccggagc tgttccgagt 780 gcatttcctt cgacgcattt cgagtcttct tggattttga caacattgtt tcgagctggg 840 ttctcagcct ctcatctagc atgcgacgaa ttaaacaagc tggtagagat cctcgaaggc 900 tcatttgaga aagaaggggg agccatcggt tatgctcctg ggtttcaagc agatgtggat 960 gataccgcaa agaccatctc cactttggct gtgcttggga gagatgccac tccccggcaa 1020 atgatcaagg tttttgaagc caatacacac tttcggactt accctggtga aagagatcca 1080 agcttgactg ccaattgcaa cgcgctctcg gctcttcttc accagccaga cgcagcaatg 1140 tacggcagcc agatccagaa gatcacaaag tttgtttgtg actactggtg gaaaagtgac 1200 ggcaaaatca aggacaagtg gaatacctgc tacttgtatc catcggtcct cctcgtcgag 1260 gtgttagtag accttgtgtc cctgttggag caaggaaagc tacccgacgt gctggatcag 1320 gagctgcaat acagggtcgc cattacgtta ttccaggcct gcttgcgacc gctacttgat 1380 caagatgctg aaggttcatg gaacaaatcc attgaagcca cagcctacgg cattctaatc 1440 cttacggagg cgcggcgagt atgctttttt gaccgtctga gtgagcctct gaatgaggct 1500

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attcgacgcg ggattgcgtt tgcagattcg atgagcggta ctgaagctca gctgaattat 1560 atatggatcg agaaagtgag ctacgcacct gctcttctga ccaaatcata cctcctcgca 1620 gctcggtggg cggcaaagtc cccgcttggc gcttccgttg gatccagcct ttggacgcct 1680 ccaagagaag gcttggataa gcacgtccgt ctattccacc aggcagagct cttcaggtcg 1740 ttgccggagt gggagctgcg cgcgtccatg atcgaggcag ccctgttcac tcctttgctg 1800 cgtgcgcata ggctggatgt atttccacgc caagacgtcg gcgaggacaa gtacctggac 1860 gttgtgccgt tcttctggac ggccgccaat aaccgcgatc gcacgtacgc atccactctg 1920 tttctgtatg acatgtgctt tatcgccatg cttaacttcc agctggatga gttcatggag 1980 gctacagcgg gaatcctctt ccgggaccat atggatgatt tgcgccaact catccacgac 2040 ctgcttgccg aaaagacgag ccccaagtca tcgggcagaa gtagccaagg aaccaaagac 2100 gcggactcgg gcatcgaaga agacgtttct atgagcgact cagcgtcaga ctcccaggac 2160 cgcagccctg aatacgacct ggtcttctct gcgctctcta ccttcaccaa acatgtcctg 2220 cagcaccctt caatccagtc agccagtgtc tgggatagga aactactcgc tcgcgagatg 2280 aaagcatacc tcctagctca tattcaacag gctgaggaca gcacgccctt gagtgagctc 2340 aaggacgtcc ctcaaaaaac tgacgtgaca cgcgtctcaa cgtccacaac gactttcttc 2400 aactgggtac gcacaacatc cgcagaccac atatcctgcc catattcatt ccatttcgtg 2460 gcgtgtcacc tcggcgccgc gctgagcccc aagggcagca acggcgactg ttacccgtca 2520 gccggtgaaa agttcctcgc ggccgccgta tgccgccatt tggccacgat gtgccgcatg 2580 tacaatgact tgggatcggc ggagcgcgac agtgacgagg gaaatttgaa ttcactcgac 2640 tttcccgagt tcgccgactc agcggggaat ggtgggattg agatccagaa agctgccttg 2700 ctcaggctgg ccgagttcga acgcgactcg tatctcgagg ctttccggcg acttcaggat 2760 gaaagcaacc gcgttcacgg accggctggt ggggatgaag ccagactcag caggcggcgc 2820 atggccatcc ttgagttctt tgcccagcag gtggacttgt atggccaggt ctacgttatt 2880 cgcgatatct cggccaggat tccaaagaac gaggttgaga agaaaaggaa actagatgat 2940 gctttcaatt ag 2952

<210> 51 <211> 2646 <212> DNA <213> Physcomitrella patens

<400> 51 atggcttcca gcaccttgat acagaatcgc tcttgtggcg ttacgtcaag catgtcttcc 60 tttcagattt ttcgagggca acctctacgt tttccaggca ctagaactcc tgctgcagtt 120 caatgcctaa agaagcgtcg atgtttgcga cctactgaat cagtcctcga gagctctcct 180 ggtagcggat cttacaggat tgtaactgga ccctccggca tcaatccttc ttcaaacggc 240 cacttgcaag aggggtccct tactcacaga cttccgatac ccatggaaaa atccattgat 300 aacttccagt ctactttgta cgtatcagac atatggtcag aaaccttgca aagaacggaa 360 Page 53

2018200459 19 Jan 2018

tgtttgttgc aggtgactga gaatgtacag atgaacgagt ggattgagga aatcagaatg 420 tacttccgaa atatgacact gggggaaata tccatgtctc catacgacac agcttgggta 480 gcgcgagtgc cagcgctgga tggctcacat ggccctcagt tccatcggtc tttgcagtgg 540 attattgata atcagctccc ggatggcgat tggggtgaac cgtctctttt ccttggatac 600 gatcgcgttt gcaacactct cgcctgtgta attgccctga aaacttgggg tgttggggct 660 cagaacgtag agcgtggaat ccagtttctg caatctaaca tctacaaaat ggaggaagat 720 gacgccaatc atatgccgat tggatttgag attgtcttcc cagcgatgat ggaagatgcc 780 aaggcactgg gactggattt accatacgat gccactatct tgcaacaaat ctcggctgaa 840 agagagaaga aaatgaaaaa gattcctatg gcgatggtgt acaagtaccc cactactttg 900 ctgcattctc tggaaggcct gcaccgggaa gtggactgga acaagctcct ccagctacag 960 tccgagaatg gctcctttct gtattcaccc gcatccactg catgcgcact tatgtacaca 1020 aaagatgtga agtgcttcga ctacttgaac cagctcctca tcaagttcga ccacgcttgt 1080 ccaaacgtgt accccgttga tctcttcgag cgtttgtgga tggtagaccg cctacaaagg 1140 ctgggaatat cccgctactt cgagcgagaa atcagagact gtctacaata tgtataccga 1200 tactggaagg attgtggtat tggctgggca agcaattcgt ccgtgcagga cgtggacgac 1260 acggccatgg ccttccgcct tctccgcaca cacggattcg acgtcaagga ggactgcttc 1320 agacagtttt tcaaagatgg tgagttcttc tgcttcgccg gccagtccag ccaagccgtc 1380 acgggaatgt tcaacctcag cagagcatcg caaacgctct tcccagggga atcactccta 1440 aaaaaggcca gaaccttttc cagaaacttt ttgagaacca agcatgaaaa caatgaatgc 1500 ttcgacaagt ggataatcac gaaggatcta gcgggcgagg tggaatacaa tctcacattc 1560 ccctggtatg ctagccttcc tcgtcttgag catcgcacct acttggacca atatgggatt 1620 gatgatatct ggattggcaa gtcgctctac aaaatgccgg ccgtcaccaa cgaagtgttt 1680 ctcaaattgg ccaaagccga cttcaacatg tgccaagctc ttcacaagaa ggaactcgag 1740 caggtcatca aatggaatgc cagctgccaa tttagagacc tcgagtttgc tagacagaaa 1800 tccgtggagt gctacttcgc aggcgctgca accatgtttg agcccgaaat ggtgcaggcg 1860 aggctcgttt gggcacgctg ttgcgtgctc accaccgttc tagacgatta cttcgatcac 1920 ggtacacctg tggaagagct tcgggttttt gtgcaggccg taaggacttg gaatcccgag 1980 ctcatcaacg gactacctga gcaagccaag attctcttta tgggactgta caagactgtg 2040 aacactatcg ccgaggaggc attcatggca cagaaacgag acgtacatca tcatctcaag 2100 cattactggg acaaattgat cacttcagct ttgaaagaag ccgaatgggc agagtccggc 2160 tacgtcccca ccttcgacga gtatatggaa gtcgctgaaa tctccgtcgc actagagccc 2220 attgtatgta gcactctctt cttcgccggc cataggctcg atgaggatgt gcttgacagt 2280 tatgactacc atcttgtcat gcatctcgtc aaccgcgtag gtcgcatcct caacgacatc 2340 caaggaatga agagggaagc cagccaaggg aagatatcga gcgtgcagat ctacatggag 2400 gagcatccaa gtgtgccttc agaggccatg gccatcgctc atctgcagga attggtcgac 2460

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aactccatgc aacagctgac atacgaagtg ctgcgcttca ctgcagtccc gaagtcctgt 2520 aagagaatcc atttaaacat ggcgaagatc atgcacgctt tctacaagga cactgatggg 2580 ttttcgtcac tgacagccat gacagggttt gtgaagaagg tgctcttcga gaatag <210> 52 <211> 1542 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 52 gccagtacct 2640 2646 atggatgctg tgacgggttt gttaactgtc ccagcaaccg ctataactat tggtggaact 60 gctgtagcat tggcggtagc gctaatcttt tggtacctga aatcctacac atcagctaga 120 agatcccaat caaatcatct tccaagagtg cctgaagtcc caggtgttcc attgttagga 180 aatctgttac aattgaagga gaaaaagcca tacatgactt ttacgagatg ggcagcgaca 240 tatggaccta tctatagtat caaaactggg gctacaagta tggttgtggt atcatctaat 300 gagatagcca aggaggcatt ggtgaccaga ttccaatcca tatctacaag gaacttatct 360 aaagccctga aagtacttac agcagataag acaatggtcg caatgtcaga ttatgatgat 420 tatcataaaa cagttaagag acacatactg accgccgtct tgggtcctaa tgcacagaaa 480 aagcatagaa ttcacagaga tatcatgatg gataacatat ctactcaact tcatgaattc 540 gtgaaaaaca acccagaaca ggaagaggta gaccttagaa aaatctttca atctgagtta 600 ttcggcttag ctatgagaca agccttagga aaggatgttg aaagtttgta cgttgaagac 660 ctgaaaatca ctatgaatag agacgaaatc tttcaagtcc ttgttgttga tccaatgatg 720 ggagcaatcg atgttgattg gagagacttc tttccatacc taaagtgggt cccaaacaaa 780 aagttcgaaa atactattca acaaatgtac atcagaagag aagctgttat gaaatcttta 840 atcaaagagc acaaaaagag aatagcgtca ggcgaaaagc taaatagtta tatcgattac 900 cttttatctg aagctcaaac tttaaccgat cagcaactat tgatgtcctt gtgggaacca 960 atcattgaat cttcagatac aacaatggtc acaacagaat gggcaatgta cgaattagct 1020 aaaaacccta aattgcaaga taggttgtac agagacatta agtccgtctg tggatctgaa 1080 aagataaccg aagagcatct atcacagctg ccttacatta cagctatttt ccacgaaaca 1140 ctgagaagac actcaccagt tcctatcatt cctctaagac atgtacatga agataccgtt 1200 ctaggcggct accatgttcc tgctggcaca gaacttgccg ttaacatcta cggttgcaac 1260 atggacaaaa acgtttggga aaatccagag gaatggaacc cagaaagatt catgaaagag 1320 aatgagacaa ttgattttca aaagacgatg gccttcggtg gtggtaagag agtttgtgct 1380 ggttccttgc aagccctttt aactgcatct attgggattg ggagaatggt tcaagagttc 1440 gaatggaaac tgaaggatat gactcaagag gaagtgaaca Page 55 cgataggcct aactacacaa 1500

2018200459 19 Jan 2018 atgttaagac cattgagagc tattatcaaa cctaggatct aa 1542 <210> 53 <211> 1530 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 53

atggcatttt tctctatgat ttcaattttg ttgggatttg ttatttcttc tttcatcttc 60 atctttttct tcaaaaagtt acttagtttt agtaggaaaa acatgtcaga agtttctact 120 ttgccaagtg ttccagtagt gcctggtttt ccagttattg ggaatttgtt gcaactaaag 180 gagaaaaagc ctcataaaac tttcactaga tggtcagaga tatatggacc tatctactct 240 ataaagatgg gttcttcatc tcttattgta ttgaacagta cagaaactgc taaggaagca 300 atggtcacta gattttcatc aatatctacc agaaaattgt caaacgccct aacagttcta 360 acctgcgata agtctatggt cgccacttct gattatgatg acttccacaa attagttaag 420 agatgtttgc taaatggact tcttggtgct aatgctcaaa agagaaaaag acactacaga 480 gatgctttga ttgaaaatgt gagttccaag ctacatgcac acgctagaga tcatccacaa 540 gagccagtta actttagagc aattttcgaa cacgaattgt ttggtgtagc attaaagcaa 600 gccttcggta aagacgtaga atccatatac gtcaaggagt taggcgtaac attatcaaaa 660 gatgaaatct ttaaggtgct tgtacatgat atgatggagg gtgcaattga tgtagattgg 720 agagatttct tcccatattt gaaatggatc cctaataagt cttttgaagc taggatacaa 780 caaaagcaca agagaagact agctgttatg aacgcactta tacaggacag attgaagcaa 840 aatgggtctg aatcagatga tgattgttac cttaacttct taatgtctga ggctaaaaca 900 ttgactaagg aacagatcgc aatccttgtc tgggaaacaa tcattgaaac agcagatact 960 accttagtca caactgaatg ggccatatac gagctagcca aacatccatc tgtgcaagat 1020 aggttgtgta aggagatcca gaacgtgtgt ggtggagaga aattcaagga agagcagttg 1080 tcacaagttc cttaccttaa cggcgttttc catgaaacct tgagaaaata ctcacctgca 1140 ccattagttc ctattagata cgcccacgaa gatacacaaa tcggtggcta ccatgttcca 1200 gctgggtccg aaattgctat aaacatctac gggtgcaaca tggacaaaaa gagatgggaa 1260 agaccagaag attggtggcc agaaagattc ttagatgatg gcaaatatga aacatctgat 1320 ttgcataaaa caatggcttt cggagctggc aaaagagtgt gtgccggtgc tctacaagcc 1380 tccctaatgg ctggtatcgc tattggtaga ttggtccaag agttcgaatg gaaacttaga 1440 gatggtgaag aggaaaatgt cgatacttat gggttaacat ctcaaaagtt atacccacta 1500 atggcaatca tcaatcctag aagatcctaa 1530

<210> 54 <211> 1578

Page 56

2018200459 19 Jan 2018 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 54

atgagtaagt ctaatagtat gaattctaca tcacacgaaa ccctttttca acaattggtc 60 ttgggtttgg accgtatgcc attgatggat gttcactggt tgatctacgt tgctttcggc 120 gcatggttat gttcttatgt gatacatgtt ttatcatctt cctctacagt aaaagtgcca 180 gttgttggat acaggtctgt attcgaacct acatggttgc ttagacttag attcgtctgg 240 gaaggtggct ctatcatagg tcaagggtac aataagttta aagactctat tttccaagtt 300 aggaaattgg gaactgatat tgtcattata ccacctaact atattgatga agtgagaaaa 360 ttgtcacagg acaagactag atcagttgaa cctttcatta atgattttgc aggtcaatac 420 acaagaggca tggttttctt gcaatctgac ttacaaaacc gtgttataca acaaagacta 480 actccaaaat tggtttcctt gaccaaggtc atgaaggaag agttggatta tgctttaaca 540 aaagagatgc ctgatatgaa aaatgacgaa tgggtagaag tagatatcag tagtataatg 600 gtgagattga tttccaggat ctccgccaga gtctttctag ggcctgaaca ctgtcgtaac 660 caggaatggt tgactactac agcagaatat tcagaatcac ttttcattac agggtttatc 720 ttaagagttg tacctcatat cttaagacca ttcatcgccc ctctattacc ttcatacagg 780 actctactta gaaacgtttc aagtggtaga agagtcatcg gtgacatcat aagatctcag 840 caaggggatg gtaacgaaga tatactttcc tggatgagag atgctgccac aggagaggaa 900 aagcaaatcg ataacattgc tcagagaatg ttaattcttt ctttagcatc aatccacact 960 actgcgatga ccatgacaca tgccatgtac gatctatgtg cttgccctga gtacattgaa 1020 ccattaagag atgaagttaa atctgttgtt ggggcttctg gctgggacaa gacagcgtta 1080 aacagatttc ataagttgga ctccttccta aaagagtcac aaagattcaa cccagtattc 1140 ttattgacat tcaatagaat ctaccatcaa tctatgacct tatcagatgg cactaacatt 1200 ccatctggaa cacgtattgc tgttccatca cacgcaatgt tgcaagattc tgcacatgtc 1260 ccaggtccaa ccccacctac tgaatttgat ggattcagat atagtaagat acgttctgat 1320 agtaactacg cacaaaagta cctattctcc atgaccgatt cttcaaacat ggctttcgga 1380 tacggcaagt atgcttgtcc aggtagattt tacgcgtcta atgagatgaa actaacatta 1440 gccattttgt tgctacaatt tgagttcaaa ctaccagatg gtaaaggtcg tcctagaaat 1500 atcactatcg attctgatat gattccagac ccaagagcta gactttgcgt cagaaaaaga 1560 tcacttagag atgaatga 1578

<210> 55 <211> 1500 <212> DNA <213> Artificial Sequence <220>

Page 57

2018200459 19 Jan 2018 <223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 55 atggaagatc ctactgtctt atatgcttgt cttgccattg cagttgcaac tttcgttgtt 60 agatggtaca gagatccatt gagatccatc ccaacagttg gtggttccga tttgcctatt 120 ctatcttaca tcggcgcact aagatggaca agacgtggca gagagatact tcaagaggga 180 tatgatggct acagaggatc tacattcaaa atcgcgatgt tagaccgttg gatcgtgatc 240 gcaaatggtc ctaaactagc tgatgaagtc agacgtagac cagatgaaga gttaaacttt 300 atggacggat taggagcatt cgtccaaact aagtacacct taggtgaagc tattcataac 360 gatccatacc atgtcgatat cataagagaa aaactaacaa gaggccttcc agccgtgctt 420 cctgatgtca ttgaagagtt gacacttgcg gttagacagt acattccaac agaaggtgat 480 gaatgggtgt ccgtaaactg ttcaaaggcc gcaagagata ttgttgctag agcttctaat 540 agagtctttg taggtttgcc tgcttgcaga aaccaaggtt acttagattt ggcaatagac 600 tttacattgt ctgttgtcaa ggatagagcc atcatcaata tgtttccaga attgttgaag 660 ccaatagttg gcagagttgt aggtaacgcc accagaaatg ttcgtagagc tgttcctttt 720 gttgctccat tggtggagga aagacgtaga cttatggaag agtacggtga agactggtct 780 gaaaaaccta atgatatgtt acagtggata atggatgaag ctgcatccag agatagttca 840 gtgaaggcaa tcgcagagag attgttaatg gtgaacttcg cggctattca tacctcatca 900 aacactatca ctcatgcttt gtaccacctt gccgaaatgc ctgaaacttt gcaaccactt 960 agagaagaga tcgaaccatt agtcaaagag gagggctgga ccaaggctgc tatgggaaaa 1020 atgtggtggt tagattcatt tctaagagaa tctcaaagat acaatggcat taacatcgta 1080 tctttaacta gaatggctga caaagatatt acattgagtg atggcacatt tttgccaaaa 1140 ggtactctag tggccgttcc agcgtattct actcatagag atgatgctgt ctacgctgat 1200 gccttagtat tcgatccttt cagattctca cgtatgagag cgagagaagg tgaaggtaca 1260 aagcaccagt tcgttaatac ttcagtcgag tacgttccat ttggtcacgg aaagcatgct 1320 tgtccaggaa gattcttcgc cgcaaacgaa ttgaaagcaa tgttggctta cattgttcta 1380 aactatgatg taaagttgcc tggtgacggt aaacgtccat tgaacatgta ttggggtcca 1440 acagttttgc ctgcaccagc aggccaagta ttgttcagaa agagacaagt tagtctataa 1500

<210> 56 <211> 1542 <212> DNA <213> Stevia rebaudiana

<400> 56 atggatgccg tcaccggttt gctgacagtt ccggcaaccg caataaccat cggcggtacg 60 gccgtcgcac tcgccgtcgc tctgatattc tggtacctca aaagctacac atctgcacgc 120 aggagccaat caaaccatct ccctcgggtt cccgaggtac ctggtgtgcc attattgggg 180 aatttattgc agttgaagga gaagaaacct tacatgactt ttacaagatg ggcggcaact 240

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tatggtccga tttattcgat taaaaccgga gcaacttcta tggtggtcgt cagttcaaat 300 gaaattgcaa aggaggcatt ggttaccaga tttcaatcta tctcaaccag aaacctatca 360 aaggcattaa aggttctcac agcagataaa accatggtgg cgatgagtga ttatgatgat 420 tatcataaga ctgtcaaacg ccatatactg accgctgttt tgggaccaaa tgctcagaag 480 aaacaccgca tccataggga catcatgatg gataatatat caacccaact tcatgaattt 540 gttaaaaata atcctgaaca agaggaagtg gatctaagga aaatattcca atccgaactt 600 tttggattag ctatgagaca agcattggga aaggatgtgg agagcttata tgttgaggat 660 cttaaaatca ccatgaaccg agacgagata tttcaggtat tggttgttga cccgatgatg 720 ggtgcaattg acgtcgactg gagagatttc ttcccgtatc taaagtgggt cccgaataaa 780 aagtttgaaa acacgatcca acaaatgtat atccggagag aagctgtgat gaagtctctt 840 attaaagaac ataaaaaacg tattgcatcc ggagagaaat taaacagcta cattgattac 900 ttgctatcgg aagcacaaac gttaaccgat caacaactac ttatgtctct atgggaacct 960 attattgaat catcagacac cactatggtt acaactgaat gggctatgta tgaacttgca 1020 aaaaacccca aacttcagga tcgtttgtat cgggatatca aaagtgtttg cgggtcagag 1080 aagattacag aagaacactt gtctcaactg ccatacataa ctgccatttt tcatgaaacc 1140 ttgagaaggc atagtccagt tcctataatt ccattaagac acgtgcatga agacacagtg 1200 ttaggagggt accatgtgcc agctggaacc gagctagcgg taaacattta tggatgtaac 1260 atggataaga atgtgtggga gaatcctgaa gaatggaatc cagagagatt catgaaggaa 1320 aatgaaacga tagatttcca gaaaacaatg gcgtttggag gtggaaagcg cgtatgtgct 1380 ggttcgcttc aagcattgtt gactgcttcc attggaattg gaagaatggt gcaagagttt 1440 gagtggaaac tgaaagayat gacccaagaa gaagttaata cgattgggct tacgacccag 1500 atgcttcgtc cactgcgggc cataataaag cccaggatat ga 1542

<210> 57 <211> 1530 <212> DNA <213> Arabidopsis thaliana

<400> 57 atggccttct tctccatgat ctccattctc cttggctttg ttatctcctc cttcatcttc 60 atcttcttct tcaagaaact tctctccttc tccagaaaga acatgtctga agtctccact 120 ctcccctctg ttccagtggt accagggttt cctgttattg ggaacttgct gcaactaaaa 180 gagaagaaac ctcacaagac tttcactaga tggtcagaga tttatggtcc tatttactct 240 ataaagatgg gttcttcttc tcttattgtc ctcaattcta ctgagactgc caaagaggcc 300 atggtgacgc ggttttcgtc tatctcaacg aggaagttgt caaatgcgtt gacagtcctt 360 acttgtgaca aatctatggt tgctactagt gattatgatg atttccacaa gttggtgaaa 420 cggtgtctct tgaacggtct tttgggtgct aatgcacaga aacgaaaaag acattacaga 480 gatgcactca ttgaaaatgt gtcttccaag ttgcatgccc atgctaggga ccatccacaa 540

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2018200459 19 Jan 2018

gaacctgtaa acttcagagc tatatttgag catgagcttt tcggtgtagc attgaagcaa 600 gcttttggga aagatgtgga atccatttat gttaaagaac tcggtgtgac tttgtcgaaa 660 gacgagatct tcaaggtttt agtacatgac atgatggaag gtgcaattga tgttgattgg 720 agagacttct tcccatactt gaaatggatt ccaaataaaa gttttgaagc aagaatccag 780 caaaagcata aacgtagact cgcggtgatg aatgctctga ttcaagatcg actgaagcag 840 aatggttcag aatcggatga tgattgctat ctcaacttct tgatgtcgga agcgaaaaca 900 ctaaccaagg agcaaattgc tatcttggtt tgggagacga ttatcgagac agctgacact 960 actttggtta caactgaatg ggccatctat gagctcgcta agcatccaag tgtccaagat 1020 cgtctgtgta aagaaatcca aaatgtctgc ggaggagaaa agttcaaaga agagcaattg 1080 tctcaagttc cttatctcaa tggagtattc catgaaacgc ttaggaaata cagtcctgct 1140 cctctagtcc ccattcgcta tgcccacgaa gatacgcaaa tcggaggcta tcatgtccct 1200 gcaggaagtg agattgcaat aaacatctat ggatgcaaca tggataagaa gcgttgggag 1260 agaccagagg actggtggcc ggagcggttt cttgatgatg gcaaatacga aacgtcggat 1320 cttcacaaga caatggcgtt tggagcggga aagagggttt gtgctggtgc tcttcaagca 1380 tctctcatgg caggcattgc cattgggagg ttagtgcaag aattcgagtg gaagcttaga 1440 gacggtgaag aagagaatgt ggatacatat ggcttgacct ctcagaagct ttatcctctt 1500 atggctatta tcaatccaag gcgttcttaa 1530

<210> 58 <211> 1578 <212> DNA <213> Gibberella fujikoroi

<400> 58 atgagtaagt ccaacagcat gaacagtacc agccatgaaa cgttattcca gcagctcgtc 60 ttaggtcttg acagaatgcc gctaatggac gttcactggc tgatctacgt ggcctttggc 120 gcttggttat gctcttatgt catccatgtc ctatcgtcct cttctacagt caaagtgccc 180 gtcgtaggct accgcagcgt ctttgagcct acatggcttc tccgtttgcg ctttgtttgg 240 gaagggggat ctatcatcgg ccaaggctac aacaaattta aagactctat cttccaggtg 300 cgaaagcttg gtaccgatat cgtcatcatc ccgccaaact acatcgatga ggtcagaaag 360 ctgtcccaag acaagactcg ctcggtcgag cccttcatca atgactttgc gggacagtat 420 acacggggca tggtctttct gcaaagtgat ttgcagaacc gtgtgattca gcagcggttg 480 acgccaaaac tcgtatcgtt gacaaaggta atgaaggagg agcttgacta tgccttgacc 540 aaagagatgc ctgacatgaa gaatgatgaa tgggttgaag tcgacatttc ttccatcatg 600 gtcaggctca tatcacgcat ctcagccaga gtgtttctcg gtccagagca ctgccgcaac 660 caagaatggt tgacgaccac tgcagagtac agcgagagcc tgttcataac tggctttatt 720 ctccgcgttg tcccccatat tctaagacca ttcatagccc cgctgctacc ctcctacaga 780 acactacttc gcaacgtctc gtcaggtcga agagttattg gagacatcat tcgctcccag 840 Page 60

2018200459 19 Jan 2018

caaggtgatg gcaacgagga catcctgtca tggatgaggg atgctgcgac aggggaagaa 900 aagcaaattg acaacattgc ccagcggatg cttatcctga gtctcgcgtc tattcacact 960 acggcaatga cgatgacgca tgctatgtat gacttatgtg cttgccctga gtacatagag 1020 cctcttagag atgaggtcaa aagtgtcgtt ggcgctagtg gttgggacaa gacggcgttg 1080 aatcgattcc acaaactcga cagctttctc aaagagtcac aacgcttcaa ccccgtgttc 1140 ctcttaacgt tcaatcgcat ttatcaccaa tccatgacac tctcagatgg caccaacatc 1200 ccatcaggca ctcgcatcgc ggttccctct cacgcgatgc ttcaggactc agcgcatgtc 1260 ccaggcccga cgccaccaac cgagtttgat ggatttagat actcaaagat tcgctcagac 1320 tcaaactatg cacagaaata tctcttctcc atgactgatt ctagtaacat ggcgtttggg 1380 tatgggaaat acgcctgccc agggcggttc tatgcatcta atgagatgaa gctgactttg 1440 gcgatactcc ttttacaatt tgagttcaag ttgccagatg ggaaaggaag accacgaaat 1500 atcactattg atagtgacat gatacctgat ccgagagcta ggctgtgcgt taggaagcga 1560 tcactgagag atgaatga 1578

<210> 59 <211> 1500 <212> DNA <213> Trametes versicolor

<400> 59 atggaggatc ccaccgtact ctacgcttgc ctcgccatcg ctgtcgctac tttcgttgtc 60 agatggtaca gagacccgct tcggtccatt cctacggttg ggggctctga ccttcccatc 120 ctctcataca tcggggcgct caggtggacc cgccgcggaa gagagatact gcaagaaggt 180 tatgatgggt atcgcggatc cacgttcaag atcgcgatgc tcgaccggtg gatcgtcatc 240 gccaacggcc caaagctcgc cgacgaggtg aggaggcgtc ctgacgaaga gctaaacttc 300 atggacggac tgggagcgtt cgtgcagacg aagtataccc ttggggaagc aatccacaat 360 gacccgtacc acgtggacat tattcgtgag aagctgacgc gaggcctccc ggcagtcctg 420 ccggacgtca tcgaggaact cacgctagcc gttcgccagt acatcccgac ggaaggagat 480 gaatgggtca gcgtgaactg ctccaaagca gcgcgggaca tcgtcgcccg ggcaagcaac 540 cgcgtctttg tcgggttgcc cgcttgccgc aaccagggtt atctcgacct cgccattgac 600 ttcaccctga gcgttgtcaa agacagggcg atcatcaata tgttcccgga gttgctgaaa 660 cctatcgtcg gacgcgtggt tggaaatgcc actaggaacg tgcgccgcgc ggtcccattc 720 gtagcgccgt tggtggagga acgtcgccgc ctcatggagg agtacggtga ggattggtcg 780 gagaaaccga acgacatgct ccagtggatc atggacgagg cagcctcgcg ggactcctcc 840 gtcaaagcga tcgctgagcg tcttctcatg gtcaactttg ccgcaattca cacgtcgtcg 900 aacaccatca cccacgctct ttaccacctc gccgagatgc cggagaccct acagccgctg 960 cgggaagaga tcgagccgct cgtcaaggaa gaaggctgga cgaaggccgc catgggcaag 1020 atgtggtggc tcgacagctt cctgcgggag tcacagcgct acaatggcat caacatcgtc 1080

Page 61

2018200459 19 Jan 2018 tccctgacgc gcatggccga caaggacata acgctcagcg acggcacgtt cctcccgaag 1140 ggcacgctcg tcgcggtccc cgcgtactcg acgcaccgcg acgacgcggt gtacgcggac 1200 gcgctggtct tcgacccgtt ccgcttctcc cgcatgcgcg cccgcgaggg cgagggcacg 1260 aagcaccagt tcgtcaacac ctccgtggag tacgtgccct tcggccacgg gaagcacgcc 1320 tgccccgggc ggttcttcgc ggccaacgag ctgaaggcga tgctcgcgta catcgtgctc 1380 aactacgacg tgaagctgcc cggcgatggc aagcgccccc tgaacatgta ctggggcccg 1440 acggtcttgc ctgctccggc tgggcaggtg ctcttccgca agaggcaggt gtcgctgtag 1500 <210> 60 <211> 1578 <212> DNA <213> Artificial Sequence

<220> <223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 60 atgggtttgt tcccattaga ggattcctac gcgctggtct ttgaaggact agcaataaca 60 ctggctttgt actatctact gtctttcatc tacaaaacat ctaaaaagac atgtacacct 120 cctaaagcat ctggtgaaat cattccaatt acaggaatca tattgaatct gctatctggc 180 tcaagtggtc tacctattat cttagcactt gcctctttag cagacagatg tggtcctatt 240 ttcaccatta ggctgggtat taggagagtg ctagtagtat caaattggga aatcgctaag 300 gagattttca ctacccacga tttgatagtt tctaatagac caaaatactt agccgctaag 360 attcttggtt tcaattatgt ttcattctct ttcgctccat acggcccata ttgggtcgga 420 atcagaaaga ttattgctac aaaactaatg tcttcttcca gacttcagaa gttgcaattt 480 gtaagagttt ttgaactaga aaactctatg aaatctatca gagaatcatg gaaggagaaa 540 aaggatgaag agggaaaggt attagttgag atgaaaaagt ggttctggga actgaatatg 600 aacatagtgt taaggacagt tgctggtaaa caatacactg gtacagttga tgatgccgat 660 gcaaagcgta tctccgagtt attcagagaa tggtttcact acactggcag atttgtcgtt 720 ggagacgctt ttccttttct aggttggttg gacctgggcg gatacaaaaa gacaatggaa 780 ttagttgcta gtagattgga ctcaatggtc agtaaatggt tagatgagca tcgtaaaaag 840 caagctaacg atgacaaaaa ggaggatatg gatttcatgg atatcatgat ctccatgaca 900 gaagcaaatt caccacttga aggatacggc actgatacta ttatcaagac cacatgtatg 960 actttgattg tttcaggagt tgatacaacc tcaatcgtac ttacttgggc cttatcactt 1020 ttgttaaaca acagagatac tttgaaaaag gcacaagagg aattagatat gtgcgtaggt 1080 aaaggaagac aagtcaacga gtctgatctt gttaacttga tatacttgga agcagtgctt 1140 aaagaggctt taagacttta cccagcagcg ttcttaggcg gaccaagagc attcttggaa 1200 gattgtactg ttgctggtta tagaattcca aagggcacct gcttgttgat taacatgtgg 1260 aaactgcata gagatccaaa catttggagt gatccttgcg aattcaagcc agaaagattt 1320

Page 62 ttgacaccta atcaaaagga tgttgatgtg atcggtatgg atttcgaatt gataccattt 1380 ggtgccggca gaagatattg tccaggtact agattggctt tacagatgtt gcatatcgta 1440 ttagcgacat tgctgcaaaa cttcgaaatg tcaacaccaa acgatgcgcc agtcgatatg 1500 actgcttctg ttggcatgac aaatgccaaa gcatcacctt tagaagtctt gctatcacct 1560 cgtgttaaat ggtcctaa 1578

2018200459 19 Jan 2018 <210> 61 <211> 1431 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 61 atgatacaag ttttaactcc aattctactc ttcctcatct tcttcgtttt ctggaaagtc 60 tacaaacatc aaaagactaa aatcaatcta ccaccaggtt ccttcggctg gccatttttg 120 ggtgaaacct tagccttact tagagcaggc tgggattctg agccagaaag attcgtaaga 180 gagcgtatca aaaagcatgg atctccactt gttttcaaga catcactatt tggagacaga 240 ttcgctgttc tttgcggtcc agctggtaat aagtttttgt tctgcaacga aaacaaatta 300 gtggcatctt ggtggccagt ccctgtaagg aagttgttcg gtaaaagttt actcacaata 360 agaggagatg aagcaaaatg gatgagaaaa atgctattgt cttacttggg tccagatgca 420 tttgccacac attatgccgt tactatggat gttgtaacac gtagacatat tgatgtccat 480 tggaggggca aggaggaagt taatgtattt caaacagtta agttgtacgc attcgaatta 540 gcttgtagat tattcatgaa cctagatgac ccaaaccaca tcgcgaaact cggtagtctt 600 ttcaacattt tcctcaaagg gatcatcgag cttcctatag acgttcctgg aactagattt 660 tactccagta aaaaggccgc agctgccatt agaattgaat tgaaaaagct cattaaagct 720 agaaaactcg aattgaagga gggtaaggcg tcttcttcac aggacttgct ttctcatcta 780 ttaacatcac ctgatgagaa tgggatgttc ttgacagaag aggaaatagt cgataacatt 840 ctacttttgt tattcgctgg tcacgatacc tctgcactat caataacact tttgatgaaa 900 accttaggtg aacacagtga tgtgtacgac aaggttttga aggaacaatt agaaatttcc 960 aaaacaaagg aggcttggga atcactaaag tgggaagata tccagaagat gaagtactca 1020 tggtcagtaa tctgtgaagt catgagattg aatcctcctg tcatagggac atacagagag 1080 gcgttggttg atatcgacta tgctggttac actatcccaa aaggatggaa gttgcattgg 1140 tcagctgttt ctactcaaag agacgaagcc aatttcgaag atgtaactag attcgatcca 1200 tccagatttg aaggggcagg ccctactcca ttcacatttg tgcctttcgg tggaggtcct 1260 agaatgtgtt taggcaaaga gtttgccagg ttagaagtgt tagcatttct ccacaacatt 1320 gttaccaact ttaagtggga tcttctaatc cctgatgaga agatcgaata tgatccaatg 1380 gctactccag ctaagggctt gccaattaga cttcatccac accaagtcta a 1431

Page 63

2018200459 19 Jan 2018 <210> 62 <211> 1578 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 62

atggagtctt tagtggttca tacagtaaat gctatctggt gtattgtaat cgtcgggatt 60 ttctcagttg gttatcacgt ttacggtaga gctgtggtcg aacaatggag aatgagaaga 120 tcactgaagc tacaaggtgt taaaggccca ccaccatcca tcttcaatgg taacgtctca 180 gaaatgcaac gtatccaatc cgaagctaaa cactgctctg gcgataacat tatctcacat 240 gattattctt cttcattatt cccacacttc gatcactgga gaaaacagta cggcagaatc 300 tacacatact ctactggatt aaagcaacac ttgtacatca atcatccaga aatggtgaag 360 gagctatctc agactaacac attgaacttg ggtagaatca cccatataac caaaagattg 420 aatcctatct taggtaacgg aatcataacc tctaatggtc ctcattgggc ccatcagcgt 480 agaattatcg cctacgagtt tactcatgat aagatcaagg gtatggttgg tttgatggtt 540 gagtctgcta tgcctatgtt gaataagtgg gaggagatgg taaagagagg cggagaaatg 600 ggatgcgaca taagagttga tgaggacttg aaagatgttt cagcagatgt gattgcaaaa 660 gcctgtttcg gatcctcatt ttctaaaggt aaggctattt tctctatgat aagagatttg 720 cttacagcta tcacaaagag aagtgttcta ttcagattca acggattcac tgatatggtc 780 tttgggagta aaaagcatgg tgacgttgat atagacgctt tagaaatgga attggaatca 840 tccatttggg aaactgtcaa ggaacgtgaa atagaatgta aagatactca caaaaaggat 900 ctgatgcaat tgattttgga aggggcaatg cgttcatgtg acggtaacct ttgggataaa 960 tcagcatata gaagatttgt tgtagataat tgtaaatcta tctacttcgc agggcatgat 1020 agtacagctg tctcagtgtc atggtgtttg atgttactgg ccctaaaccc atcatggcaa 1080 gttaagatcc gtgatgaaat tctgtcttct tgcaaaaatg gtattccaga tgccgaaagt 1140 atcccaaacc ttaaaacagt gactatggtt attcaagaga caatgagatt ataccctcca 1200 gcaccaatcg tcgggagaga agcctctaaa gatatcagat tgggcgatct agttgttcct 1260 aaaggcgtct gtatatggac actaatacca gctttacaca gagatcctga gatttgggga 1320 ccagatgcaa acgatttcaa accagaaaga ttttctgaag gaatttcaaa ggcttgtaag 1380 tatcctcaaa gttacattcc atttggtctg ggtcctagaa catgcgttgg taaaaacttt 1440 ggcatgatgg aagtaaaggt tcttgtttcc ctgattgtct ccaagttctc tttcactcta 1500 tctcctacct accaacatag tcctagtcac aaacttttag tagaaccaca acatggggtg 1560 gtaattagag tggtttaa 1578

<210> 63 <211> 1590 <212> DNA

Page 64

2018200459 19 Jan 2018 <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 63

atgtacttcc tactacaata cctcaacatc acaaccgttg gtgtctttgc cacattgttt 60 ctctcttatt gtttacttct ctggagaagt agagcgggta acaaaaagat tgccccagaa 120 gctgccgctg catggcctat tatcggccac ctccacttac ttgcaggtgg atcccatcaa 180 ctaccacata ttacattggg taacatggca gataagtacg gtcctgtatt cacaatcaga 240 ataggcttgc atagagctgt agttgtctca tcttgggaaa tggcaaagga atgttcaaca 300 gctaatgatc aagtgtcttc ttcaagacct gaactattag cttctaagtt gttgggttat 360 aactacgcca tgtttggttt ttcaccatac ggttcatact ggagagaaat gagaaagatc 420 atctctctcg aattactatc taattccaga ttggaactat tgaaagatgt tagagcctca 480 gaagttgtca catctattaa ggaactatac aaattgtggg cggaaaagaa gaatgagtca 540 ggattggttt ctgtcgagat gaaacaatgg ttcggagatt tgactttaaa cgtgatcttg 600 agaatggtgg ctggtaaaag atacttctcc gcgagtgacg cttcagaaaa caaacaggcc 660 cagcgttgta gaagagtctt cagagaattc ttccatctct ccggcttgtt tgtggttgct 720 gatgctatac cttttcttgg atggctcgat tggggaagac acgagaagac cttgaaaaag 780 accgccatag aaatggattc catcgcccag gagtggcttg aggaacatag acgtagaaaa 840 gattctggag atgataattc tacccaagat ttcatggacg ttatgcaatc tgtgctagat 900 ggcaaaaatc taggcggata cgatgctgat acgattaaca aggctacatg cttaactctt 960 atatcaggtg gcagtgatac tactgtagtt tctttgacat gggctcttag tcttgtgtta 1020 aacaatagag atactttgaa aaaggcacag gaagagttag acatccaagt cggtaaggaa 1080 agattggtta acgagcaaga catcagtaag ttagtttact tgcaagcaat agtaaaagag 1140 acactcagac tttatccacc aggtcctttg ggtggtttga gacaattcac tgaagattgt 1200 acactaggtg gctatcacgt ttcaaaagga actagattaa tcatgaactt atccaagatt 1260 caaaaagatc cacgtatttg gtctgatcct actgaattcc aaccagagag attccttacg 1320 actcataaag atgtcgatcc acgtggtaaa cactttgaat tcattccatt cggtgcagga 1380 agacgtgcat gtcctggtat cacattcgga ttacaagtac tacatctaac attggcatct 1440 ttcttgcatg cgtttgaatt ttcaacacca tcaaatgagc aggttaacat gagagaatca 1500 ttaggtctta cgaatatgaa atctacccca ttagaagttt tgatttctcc aagactatcc 1560 cttaattgct tcaaccttat gaaaatttga 1590

<210> 64 <211> 1440 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic Page 65

2018200459 19 Jan 2018 polynucleotide <400> 64

atggaaccta acttttactt gtcattacta ttgttgttcg tgaccttcat ttctttaagt 60 ctgtttttca tcttttacaa acaaaagtcc ccattgaatt tgccaccagg gaaaatgggt 120 taccctatca taggtgaaag tttagaattc ctatccacag gctggaaggg acatcctgaa 180 aagttcatat ttgatagaat gcgtaagtac agtagtgagt tattcaagac ttctattgta 240 ggcgaatcca cagttgtttg ctgtggggca gctagtaaca aattcctatt ctctaacgaa 300 aacaaactgg taactgcctg gtggccagat tctgttaaca aaatcttccc aacaacttca 360 ctggattcta atttgaagga ggaatctata aagatgagaa agttgctgcc acagttcttc 420 aaaccagaag cacttcaaag atacgtcggc gttatggatg taatcgcaca aagacatttt 480 gtcactcact gggacaacaa aaatgagatc acagtttatc cacttgctaa aagatacact 540 ttcttgcttg cgtgtagact gttcatgtct gttgaggatg aaaatcatgt ggcgaaattc 600 tcagacccat tccaactaat cgctgcaggc atcatttcac ttcctatcga tcttcctggt 660 actccattca acaaggccat aaaggcttca aatttcatta gaaaagagct gataaagatt 720 atcaaacaaa gacgtgttga tctggcagag ggtacagcat ctccaaccca ggatatcttg 780 tcacatatgc tattaacatc tgatgaaaac ggtaaatcta tgaacgagtt gaacattgcc 840 gacaagattc ttggactatt gataggaggc cacgatacag cttcagtagc ttgcacattt 900 ctagtgaagt acttaggaga attaccacat atctacgata aagtctacca agagcaaatg 960 gaaattgcca agtccaaacc tgctggggaa ttgttgaatt gggatgactt gaaaaagatg 1020 aagtattcat ggaatgtggc atgtgaggta atgagattgt caccaccttt acaaggtggt 1080 tttagagagg ctataactga ctttatgttt aacggtttct ctattccaaa agggtggaag 1140 ttatactggt ccgccaactc tacacacaaa aatgcagaat gtttcccaat gcctgagaaa 1200 ttcgatccta ccagatttga aggtaatggt ccagcgcctt atacatttgt accattcggt 1260 ggaggcccta gaatgtgtcc tggaaaggaa tacgctagat tagaaatctt ggttttcatg 1320 cataatctgg tcaaacgttt taagtgggaa aaggttattc cagacgaaaa gattattgtc 1380 gatccattcc caatcccagc taaagatctt ccaatccgtt tgtatcctca caaagcttaa 1440

<210> 65 <211> 1572 <212> DNA <213> Stevia rebaudiana

<400> 65 atgggtctct tccctttgga agatagttac gcactcgtct ttgaaggttt agcaataact 60 actctagctc tctactactt attatccttc atctataaaa cctctaaaaa gacttgtact 120 ccacctaaag caagcggtga gcaccctata acaggccact taaaccttct tagtggttca 180 tccggtcttc cccatctagc cttagcatct ttggctgacc gatgtgggcc catattcacc 240 atccgacttg gcatacgtag agttttggtg gttagtaatt gggaaattgc taaggagatc 300 ttcactaccc atgatttgat tgtttcaaac cgtcccaaat Page 66 acctcgctgc aaagattttg 360

2018200459 19 Jan 2018

ggattcaact atgtgtcctt ttcgtttgct ccatatggcc cctattgggt tggaatccgt 420 aagatcatcg ccacaaaact gatgtcaagt agcaggctcc agaagcttca gtttgtccga 480 gttttcgaac tagaaaactc catgaaaagc atacgcgagt cttggaaaga gaaaaaagac 540 gaagaaggta aagtgttggt ggagatgaaa aaatggtttt gggaattgaa tatgaatata 600 gttcttagaa ctgttgctgg taaacagtac actggaactg ttgatgatgc ggatgcgaag 660 aggattagtg aattgtttag agaatggttt cattacacag gaaggtttgt tgtgggagat 720 gcttttcctt ttcttgggtg gttggatttg ggtggatata agaagaccat ggaactagtg 780 gcttccagac tagattccat ggtctcaaaa tggttagacg agcatcgcaa aaagcaggct 840 aacgacgaca aaaaagagga catggatttc atggacatca tgatatcgat gactgaagcc 900 aattcccctt tggagggtta tggtacggat acaataatta aaaccacttg catgactctt 960 attgtcagtg gtgtagatac aacctccatc gtgctaactt gggcactctc gttactactg 1020 aacaaccgtg acactcttaa gaaagctcaa gaagagctag acatgtgtgt gggaaaaggt 1080 cgacaagtaa acgaatcaga tctagtaaac ctaatctacc ttgaagccgt attaaaagaa 1140 gcattgcgac tatacccagc agcattcctt ggaggtccta gagccttttt agaagactgc 1200 accgtggcag ggtaccgtat cccaaaaggc acatgtctac ttattaacat gtggaaactt 1260 catcgtgatc caaacatatg gtcagaccca tgtgagttta aaccagagag gttcttaacc 1320 ccaaaccaaa aggacgtaga tgttattgga atggattttg agttaatccc atttggtgcg 1380 ggaagaaggt attgtccagg gacacgtttg gcattacaaa tgttacacat agttctggcc 1440 actctactac aaaactttga gatgtcaact ccaaatgatg cacccgttga tatgaccgcg 1500 agtgttggaa tgacaaatgc gaaggcaagt ccacttgaag ttctactttc gccacgtgtt 1560 aagtggtcat ag 1572

<210> 66 <211> 1431 <212> DNA <213> Stevia rebaudiana

<400> 66 atgattcaag ttctaacacc gatccttctc ttcctcattt tcttcgtttt ctggaaggtt 60 tacaagcacc agaaaaccaa aatcaatctt ccaccgggaa gcttcggatg gccatttctg 120 ggcgaaactc tggcactcct acgtgcaggt tgggactcag agccggagag atttgttcgt 180 gaacggatca agaaacacgg aagtcctcta gtgtttaaga cgtcgttgtt tggcgaccgt 240 tttgcggtgt tgtgtggacc tgccggaaac aagttcctgt tctgcaacga gaacaagctg 300 gtggcgtcgt ggtggccggt tccggtgagg aagcttttcg gcaagtctct gctcacgatt 360 cgtggtgatg aagctaagtg gatgaggaag atgttgttat cgtatctcgg tcctgatgct 420 ttcgcaactc attatgccgt caccatggac gtcgtcaccc gtcggcatat cgacgttcat 480 tggcgaggga aggaagaggt gaacgtattc caaaccgtta agttatatgc ctttgagctt 540 gcatgtcgtt tattcatgaa cctagacgac ccaaaccaca ttgcaaaact cggttccttg 600

Page 67

2018200459 19 Jan 2018 ttcaacattt tcttgaaagg catcattgag cttccaatcg acgtcccagg gacacgattt 660 tatagctcca aaaaagcagc agcagctatc aggattgaac taaaaaaatt gattaaagca 720 agaaaactgg aactgaaaga agggaaggca tcatcttcac aagacctctt atcacatttg 780 cttacatctc cagatgaaaa tggtatgttt ctaaccgaag aagagattgt agacaacatc 840 ttgttactac tctttgcggg tcatgatacc tcggctcttt caatcacttt gctcatgaag 900 actcttggcg aacattctga tgtttatgac aaggtgttaa aagagcaact agagatatcg 960 aagacgaaag aagcatggga gtccctgaaa tgggaggaca tacaaaagat gaaatactcc 1020 tggagtgtta tatgtgaagt catgagacta aatccacctg ttataggaac ctatagagag 1080 gcccttgtgg atattgatta tgcgggttat accatcccca aaggatggaa gctgcactgg 1140 agtgctgtat cgacacaaag ggacgaggct aactttgaag acgtaacacg ttttgaccca 1200 tcacggtttg aaggcgcagg accgactcca ttcacctttg ttccgtttgg aggggggcct 1260 agaatgtgtt tagggaaaga atttgctcga ttggaagtac ttgcgtttct tcacaatatt 1320 gtcaccaatt tcaaatggga cctgttgata cctgatgaga aaatagaata tgatcccatg 1380 gctaccccag caaaggggct tccaattcgt cttcatcccc atcaagtttg a 1431 <210> 67 <211> 1578 <212> DNA <213> Arabidopsis thaliana <400> 67 atggagagtt tggttgttca tacggtaaat gcaatttggt gcatagttat tgtcggaatc 60 ttcagcgtag gttatcatgt gtatggaaga gcggtggtgg agcagtggag gatgcggagg 120 agtttaaagt tgcaaggcgt gaagggtcct ccaccgtcga tctttaacgg caatgtgtcg 180 gagatgcaac ggattcagtc ggaggctaaa cactgttccg gcgataacat catttctcat 240 gactattctt cttctctatt tcctcatttc gatcactggc gaaaacaata cggaaggatt 300 tacacatact caacggggtt aaagcagcac ctttacataa accacccgga aatggtgaag 360 gagcttagcc aaaccaacac acttaacctt ggtagaatca ctcacatcac caaacgcctt 420 aaccccattc tcggcaatgg catcatcacc tctaatgggc ctcattgggc ccatcaacgt 480 cgtatcattg cctatgagtt tacccacgac aaaatcaagg gaatggttgg tttaatggtg 540 gaatctgcca tgccaatgtt gaacaaatgg gaagagatgg tgaaaagagg aggagaaatg 600 ggttgtgaca taagagtgga cgaagacctt aaggatgtct cagctgatgt catcgctaag 660 gcttgctttg ggagctcttt ttcaaaaggc aaagcaatat tctctatgat tagggatctt 720 ttaaccgcca ttactaaacg aagcgtcctc ttcagattca atggcttcac tgatatggtg 780 tttggaagta agaagcatgg tgatgtggat attgatgcgc ttgagatgga attagaatct 840 tctatatggg aaacggttaa ggagagggaa attgaatgta aggatactca caagaaggat 900 ctaatgcagt tgatactcga gggagcgatg cgaagctgcg atggtaactt gtgggacaag 960 tcagcctata gacggtttgt ggtggacaat tgcaagagca tctatttcgc cggacatgat 1020

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2018200459 19 Jan 2018

tcaaccgcag tctcagtgtc ttggtgcctt atgctcctcg ctctcaatcc tagttggcag 1080 gttaaaattc gcgatgaaat cttgagttct tgcaagaatg gcattcccga cgcagaatca 1140 attcctaatc tcaaaacggt gacaatggta atacaagaaa caatgagact atacccacca 1200 gcaccaatcg tgggaagaga agcatccaaa gacataagac ttggagacct tgtggtgcca 1260 aaaggagtgt gcatttggac actcattcct gccttacacc gagaccccga gatctgggga 1320 ccagacgcaa acgacttcaa gccagagagg tttagtgagg gaatctctaa ggcttgcaaa 1380 taccctcagt catacatccc atttggcctt ggaccaagaa catgcgtagg caaaaacttt 1440 ggtatgatgg aagtgaaagt gcttgtttca cttattgtct caaagttcag ttttactctt 1500 tccccgactt atcagcactc tccaagccat aaactccttg tagagcctca acatggtgtt 1560 gtcattaggg ttgtttga 1578

<210> 68 <211> 1590 <212> DNA <213> Vitis vinifera

<400> 68 atgtatttcc ttctccaata cctaaacatc accacggtcg gagtctttgc cacacttttc 60 ctttcctact gtctattatt atggaggtct agagctggta acaaaaaaat agcacctgaa 120 gctgctgctg catggcccat aatcggtcac ctacacctgt tagctggtgg ttctcatcag 180 cttccccaca taaccttggg aaacatggcc gacaaatatg gaccggtctt cacaattcgg 240 attgggttgc atcgagctgt ggtggtaagt tcttgggaga tggctaaaga atgctcgacc 300 gccaatgacc aggtttcatc ctcgcgtccc gaacttttag cctcaaaact tttgggctac 360 aactacgcca tgtttggttt ctctccatac ggttcttact ggcgtgaaat gcgcaagata 420 atcagcctag agctactctc taacagccgc ttagagctgc tgaaggacgt ccgagcttca 480 gaagtggtga catccataaa agagctatac aagctctggg cagagaaaaa aaatgaatcg 540 ggccttgtct cggtggagat gaagcagtgg tttggagact tgactctgaa cgtaattctt 600 aggatggtgg cagggaagcg ttatttcagt gcttcagatg caagtgaaaa taaacaggcg 660 cagaggtgcc ggagagtgtt cagggaattc tttcatttgt cagggctctt tgtggtggcg 720 gacgctattc catttcttgg atggctcgac tgggggagac atgagaaaac cctaaagaag 780 acagcaatag aaatggacag tattgctcaa gaatggttag aggagcaccg tcggaggaaa 840 gactccggtg atgataatag tacgcaagac ttcatggatg tgatgcagtc agttcttgat 900 ggcaaaaacc ttggtggtta cgacgctgat accatcaata aagccacatg cctgactcta 960 atctccggag gtagcgacac aactgttgtc tctctaacat gggcactctc tcttgtacta 1020 aacaaccgtg acaccttaaa aaaagctcaa gaagaattag acatccaagt tggtaaggaa 1080 agattagtga atgaacaaga tataagtaag ttggtctatc tccaagccat tgttaaagag 1140 acattacggt tatatccacc aggaccactt ggaggactac gccaatttac cgaggattgc 1200 accttgggtg gataccatgt ctctaaaggc acccgtttaa Page 69 taatgaacct ttcgaagatc 1260

2018200459 19 Jan 2018

caaaaggatc caagaatttg gtcagatccg acagaattcc aaccagagag gtttctcacc 1320 acccataaag atgttgatcc tcggggaaaa cattttgagt ttataccatt tggagctggt 1380 cgaagagcat gtccaggaat aacttttggt cttcaagtat tacatttaac attggctagt 1440 ttcttacatg cgtttgaatt ttcaactcca tcaaatgaac aggtcaatat gcgcgagagc 1500 cttggactta caaatatgaa atctacccca cttgaagttc tcatttctcc acgcttatca 1560 ttgaattgtt ttaacctaat gaagatataa 1590

<210> 69 <211> 1440 <212> DNA <213> Medicago trunculata <400> 69

atggagccta atttctatct ctcccttctc cttctctttg tcactttcat atctctctct 60 ctttttttca tattctacaa acagaaatct ccattaaatt tgccacctgg taaaatgggt 120 tacccaatca taggtgaaag ccttgagttc ttatcaacag gatggaaagg acatcctgaa 180 aaattcattt tcgaccgtat gcgtaaatat tcctcagaac tctttaaaac atcaatcgta 240 ggagaatcta cggtggtttg ttgcggagca gcaagtaaca agtttttgtt ttcaaacgag 300 aataaacttg tgactgcatg gtggccagat agtgtaaaca aaatcttccc tactacttct 360 cttgactcta acttgaagga agaatccatc aagatgagaa aattgcttcc acaattcttt 420 aaacccgaag ctctacaacg ttatgttggt gtcatggatg ttattgctca aagacatttt 480 gttactcatt gggataataa aaatgaaatc accgtctacc ccttggccaa gaggtacacc 540 tttttgttag cttgtcggtt gttcatgagc gttgaagacg agaatcatgt agcaaaattt 600 agtgatccat ttcagttaat tgcggccgga atcatatctc taccaattga tttgccagga 660 acaccattca acaaagctat aaaggcctca aactttataa gaaaggagtt gattaagatc 720 ataaagcaaa ggagggtaga tttggcagaa gggacagcat caccaacaca agatatattg 780 tctcacatgt tgttgacaag tgatgaaaat ggaaagagta tgaatgaact taatattgct 840 gataagattc ttggcctttt gatcggagga catgacactg ctagcgtcgc atgcactttc 900 cttgtcaaat atctcggcga gttacctcac atttatgata aagtctatca agagcaaatg 960 gaaattgcaa aatcgaaacc agcaggagaa ttgttgaatt gggatgacct gaagaaaatg 1020 aaatactctt ggaacgtagc ttgtgaagta atgagacttt cccctccact ccaaggaggt 1080 ttcagggaag ccatcactga ctttatgttc aatggattct caattcctaa gggatggaag 1140 ctttattgga gtgcaaattc aacacataag aacgcagaat gttttcccat gccagagaaa 1200 tttgacccaa caagatttga aggaaatgga ccagctcctt atacttttgt tccctttggt 1260 ggaggaccaa ggatgtgtcc tggaaaagag tatgcaagat tagaaatact tgttttcatg 1320 cacaatttgg tgaaaaggtt taagtgggaa aaggtgattc cagatgagaa gattattgtt 1380 gatccattcc ccatccctgc aaaggatctt ccaattcgcc tttatccaca caaagcttaa 1440

Page 70

2018200459 19 Jan 2018 <210> 70 <211> 2133 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 70 atgcaatcag attcagtcaa agtctctcca tttgatttgg tttccgctgc tatgaatggc 60 aaggcaatgg aaaagttgaa cgctagtgaa tctgaagatc caacaacatt gcctgcacta 120 aagatgctag ttgaaaatag agaattgttg acactgttca caacttcctt cgcagttctt 180 attgggtgtc ttgtatttct aatgtggaga cgttcatcct ctaaaaagct ggtacaagat 240 ccagttccac aagttatcgt tgtaaagaag aaagagaagg agtcagaggt tgatgacggg 300 aaaaagaaag tttctatttt ctacggcaca caaacaggaa ctgccgaagg ttttgctaaa 360 gcattagtcg aggaagcaaa agtgagatat gaaaagacct ctttcaaggt tatcgatcta 420 gatgactacg ctgcagatga tgatgaatat gaggaaaaac tgaaaaagga atccttagcc 480 ttcttcttct tggccacata cggtgatggt gaacctactg ataatgctgc taacttctac 540 aagtggttca cagaaggcga cgataaaggt gaatggctga aaaagttaca atacggagta 600 tttggtttag gtaacagaca atatgaacat ttcaacaaga tcgctattgt agttgatgat 660 aaacttactg aaatgggagc caaaagatta gtaccagtag gattagggga tgatgatcag 720 tgtatagaag atgacttcac cgcctggaag gaattggtat ggccagaatt ggatcaactt 780 ttaagggacg aagatgatac ttctgtgact accccataca ctgcagccgt attggagtac 840 agagtggttt accatgataa accagcagac tcatatgctg aagatcaaac ccatacaaac 900 ggtcatgttg ttcatgatgc acagcatcct tcaagatcta atgtggcttt caaaaaggaa 960 ctacacacct ctcaatcaga taggtcttgt actcacttag aattcgatat ttctcacaca 1020 ggactgtctt acgaaactgg cgatcacgtt ggcgtttatt ccgagaactt gtccgaagtt 1080 gtcgatgaag cactaaaact gttagggtta tcaccagaca catacttctc agtccatgct 1140 gataaggagg atgggacacc tatcggtggt gcttcactac caccaccttt tcctccttgc 1200 acattgagag acgctctaac cagatacgca gatgtcttat cctcacctaa aaaggtagct 1260 ttgctggcat tggctgctca tgctagtgat cctagtgaag ccgataggtt aaagttcctg 1320 gcttcaccag ccggaaaaga tgaatatgca caatggatcg tcgccaacca acgttctttg 1380 ctagaagtga tgcaaagttt tccatctgcc aagcctccat taggtgtgtt cttcgcagca 1440 gtagctccac gtttacaacc aagatactac tctatcagtt catctcctaa gatgtctcct 1500 aacagaatac atgttacatg tgctttggtg tacgagacta ctccagcagg cagaattcac 1560 agaggattgt gttcaacctg gatgaaaaat gctgtccctt taacagagtc acctgattgc 1620 tctcaagcat ccattttcgt tagaacatca aatttcagac ttccagtgga tccaaaagtt 1680 ccagtcatta tgataggacc aggcactggt cttgccccat tcaggggctt tcttcaagag 1740 agattggcct tgaaggaatc tggtacagaa ttgggttctt ctatcttttt ctttggttgc 1800

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2018200459 19 Jan 2018

cgtaatagaa aagttgactt tatctacgag gacgagctta acaattttgt tgagacagga 1860 gcattgtcag aattgatcgt cgcattttca agagaaggga ctgccaaaga gtacgttcag 1920 cacaagatga gtcaaaaagc ctccgatata tggaaacttc taagtgaagg tgcctatctt 1980 tatgtctgtg gcgatgcaaa gggcatggcc aaggatgtcc atagaactct gcatacaatt 2040 gttcaggaac aagggagtct ggattcttcc aaggctgaat tgtacgtcaa aaacttacag 2100 atgtctggaa gatacttaag agatgtttgg taa 2133

<210> 71 <211> 2079 <212> DNA <213> Artificial Sequence

<220> <223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 71 atgacttctg cactttatgc ctccgatctt ttcaaacaat tgaaaagtat catgggaacg 60 gattctttgt ccgatgatgt tgtattagtt attgctacaa cttctctggc actggttgct 120 ggtttcgttg tcttattgtg gaaaaagacc acggcagatc gttccggcga gctaaagcca 180 ctaatgatcc ctaagtctct gatggcgaaa gatgaggatg atgacttaga tctaggttct 240 ggaaaaacga gagtctctat cttcttcggc acacaaaccg gaacagccga aggattcgct 300 aaagcacttt cagaagagat caaagcaaga tacgaaaagg cggctgtaaa agtaatcgat 360 ttggatgatt acgctgccga tgatgaccaa tatgaggaaa agttgaaaaa ggaaacattg 420 gctttctttt gtgtagccac gtatggtgat ggtgaaccaa ccgataacgc cgcaagattc 480 tacaagtggt ttactgaaga gaacgaaaga gatatcaagt tgcagcaact tgcttacggc 540 gtttttgcct taggtaacag acaatacgag cactttaaca agataggtat tgtcttagat 600 gaagagttat gcaaaaaggg tgcgaagaga ttgattgaag tcggtttagg agatgatgat 660 caatctatcg aggatgactt taatgcatgg aaggaatctt tgtggtctga attagataag 720 ttacttaagg acgaagatga taaatccgtt gccactccat acacagccgt cattccagaa 780 tatagagtag ttactcatga tccaagattc acaacacaga aatcaatgga aagtaatgtg 840 gctaatggta atactaccat cgatattcat catccatgta gagtagacgt tgcagttcaa 900 aaggaattgc acactcatga atcagacaga tcttgcatac atcttgaatt tgatatatca 960 cgtactggta tcacttacga aacaggtgat cacgtgggtg tctacgctga aaaccatgtt 1020 gaaattgtag aggaagctgg aaagttgttg ggccatagtt tagatcttgt tttctcaatt 1080 catgccgata aagaggatgg ctcaccacta gaaagtgcag tgcctccacc atttccagga 1140 ccatgcaccc taggtaccgg tttagctcgt tacgcggatc tgttaaatcc tccacgtaaa 1200 tcagctctag tggccttggc tgcgtacgcc acagaacctt ctgaggcaga aaaactgaaa 1260 catctaactt caccagatgg taaggatgaa tactcacaat ggatagtagc tagtcaacgt 1320 tctttactag aagttatggc tgctttccca tccgctaaac Page 72 ctcctttggg tgttttcttc 1380

2018200459 19 Jan 2018 gccgcaatag cgcctagact gcaaccaaga tactattcaa tttcatcctc acctagactg 1440 gcaccatcaa gagttcatgt cacatccgct ttagtgtacg gtccaactcc tactggtaga 1500 atccataagg gcgtttgttc aacatggatg aaaaacgcgg ttccagcaga gaagtctcac 1560 gaatgttctg gtgctccaat ctttatcaga gcctccaact tcaaactgcc ttccaatcct 1620 tctactccta ttgtcatggt cggtcctggt acaggtcttg ctccattcag aggtttctta 1680 caagagagaa tggccttaaa ggaggatggt gaagagttgg gatcttcttt gttgtttttc 1740 ggctgtagaa acagacaaat ggatttcatc tacgaagatg aactgaataa ctttgtagat 1800 caaggagtta tttcagagtt gataatggct ttttctagag aaggtgctca gaaggagtac 1860 gtccaacaca aaatgatgga aaaggccgca caagtttggg acttaatcaa agaggaaggc 1920 tatctatatg tctgtggtga tgcaaagggt atggcaagag atgttcacag aacacttcat 1980 actatagtcc aggaacagga aggcgttagt tcttctgaag cggaagcaat tgtgaaaaag 2040 ttacaaacag agggaagata cttgagagat gtgtggtaa 2079 <210> 72 <211> 2142 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 72

atggcagaat tagatacact tgatatagta gtattaggtg ttatcttttt gggtactgtg 60 gcatacttta ctaagggtaa attgtggggt gttaccaagg atccatacgc taacggattc 120 gctgcaggtg gtgcttccaa gcctggcaga actagaaaca tcgtcgaagc tatggaggaa 180 tcaggtaaaa actgtgttgt tttctacggc agtcaaacag gtacagcgga ggattacgca 240 tcaagacttg caaaggaagg aaagtccaga ttcggtttga acactatgat cgccgatcta 300 gaagattatg acttcgataa cttagacact gttccatctg ataacatcgt tatgtttgta 360 ttggctactt acggtgaagg cgaaccaaca gataacgccg tggatttcta tgagttcatt 420 actggcgaag atgcctcttt caatgagggc aacgatcctc cactaggtaa cttgaattac 480 gttgcgttcg gtctgggcaa caatacctac gaacactaca actcaatggt caggaacgtt 540 aacaaggctc tagaaaagtt aggagctcat agaattggag aagcaggtga gggtgacgac 600 ggagctggaa ctatggaaga ggacttttta gcttggaaag atccaatgtg ggaagccttg 660 gctaaaaaga tgggcttgga ggaaagagaa gctgtatatg aacctatttt cgctatcaat 720 gagagagatg atttgacccc tgaagcgaat gaggtatact tgggagaacc taataagcta 780 cacttggaag gtacagcgaa aggtccattc aactcccaca acccatatat cgcaccaatt 840 gcagaatcat acgaactttt ctcagctaag gatagaaatt gtctgcatat ggaaattgat 900 atttctggta gtaatctaaa gtatgaaaca ggcgaccata tcgcgatctg gcctaccaac 960 ccaggtgaag aggtcaacaa atttcttgac attctagatc Page 73 tgtctggtaa gcaacattcc 1020

2018200459 19 Jan 2018

gtcgtaacag tgaaagcctt agaacctaca gccaaagttc cttttccaaa tccaactacc 1080 tacgatgcta tattgagata ccatctggaa atatgcgctc cagtttctag acagtttgtc 1140 tcaactttag cagcattcgc ccctaatgat gatatcaaag ctgagatgaa ccgtttggga 1200 tcagacaaag attacttcca cgaaaagaca ggaccacatt actacaatat cgctagattt 1260 ttggcctcag tctctaaagg tgaaaaatgg acaaagatac cattttctgc tttcatagaa 1320 ggccttacaa aactacaacc aagatactat tctatctctt cctctagttt agttcagcct 1380 aaaaagatta gtattactgc tgttgtcgaa tctcagcaaa ttccaggtag agatgaccca 1440 ttcagaggtg tagcgactaa ctacttgttc gctttgaagc agaaacaaaa cggtgatcca 1500 aatccagctc cttttggcca atcatacgag ttgacaggac caaggaataa gtatgatggt 1560 atacatgttc cagtccatgt aagacattct aactttaagc taccatctga tccaggcaaa 1620 cctattatca tgatcggtcc aggtaccggt gttgcccctt ttagaggctt cgtccaagag 1680 agggcaaaac aagccagaga tggtgtagaa gttggtaaaa cactgctgtt ctttggatgt 1740 agaaagagta cagaagattt catgtatcaa aaagagtggc aagagtacaa ggaagctctt 1800 ggcgacaaat tcgaaatgat tacagctttt tcaagagaag gatctaaaaa ggtttatgtt 1860 caacacagac tgaaggaaag atcaaaggaa gtttctgatc ttctatccca aaaagcatac 1920 ttctacgttt gcggagacgc cgcacatatg gcacgtgaag tgaacactgt gttagcacag 1980 atcatagcag aaggccgtgg tgtatcagaa gccaagggtg aggaaattgt caaaaacatg 2040 agatcagcaa atcaatacca agtgtgttct gatttcgtaa ctttacactg taaagagaca 2100 acatacgcga attcagaatt gcaagaggat gtctggagtt aa 2142

<210> 73 <211> 2133 <212> DNA <213> Stevia rebaudiana

<400> 73 atgcaatcag attccgtaaa agtgtcgccg ttcgatctcg tatctgcagc tatgaacgga 60 aaagcaatgg agaaattgaa cgcatcggaa tcggaagatc cgacgacgct accggcgttg 120 aagatgctgg tggagaatcg cgagctgctg acactgttta cgacgtcgtt tgctgtattg 180 atcggatgtc tcgtgttttt gatgtggcgg agatcgtcct cgaagaaact ggttcaggat 240 ccggtgccgc aggtaatcgt tgttaagaag aaagagaagg agtctgaggt tgatgatggc 300 aagaagaaag tttcgatatt ctacggaact caaacaggaa ccgctgaagg ttttgccaag 360 gcacttgtag aggaagctaa agttcgatat gaaaagacat cctttaaagt tattgatctg 420 gatgattatg ctgctgatga cgatgagtat gaggagaagc ttaagaaaga atctttggcg 480 tttttctttt tggcaacgta tggagatggt gaaccaacag ataatgcagc caatttttac 540 aaatggttta cagagggaga tgacaaaggc gaatggctga agaaacttca atatggcgtg 600 tttggcctcg gtaacagaca atatgagcat ttcaataaga ttgcaatagt ggttgatgac 660 aaactcacag aaatgggcgc aaaacgcctt gttcctgtgg gtcttggaga tgacgatcaa 720

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tgtatagaag atgactttac agcatggaaa gagttagtgt ggcccgagtt ggatcaattg 780 ttgcgtgatg aggatgacac gagtgttacg actccttaca ctgctgcggt tttggaatac 840 cgagttgtat atcatgataa acctgcagac tcgtatgcag aagatcaaac tcatacaaat 900 ggtcatgttg ttcatgatgc tcaacatcca tctagatcca atgtggcatt taaaaaggaa 960 ttgcacacct ctcaatctga ccggtcttgc actcatttgg aatttgatat ctctcacacc 1020 gggctatcat acgagacggg ggatcatgtt ggtgtctaca gtgagaatct aagtgaagtt 1080 gtagatgaag ctttaaaatt actcggtttg tcacccgaca cttatttctc agtccatgct 1140 gacaaggaag acggaacacc tattggcggc gcctccttgc cgccaccttt ccctccatgc 1200 actttaagag atgcattaac gcgctacgca gatgttttga gttctcctaa aaaggttgct 1260 ttgcttgctc tggctgctca tgcttctgat cctagcgaag ccgatcgatt aaaatttcta 1320 gcatctccgg ctggcaagga tgaatatgct caatggatag ttgcaaacca aagaagtctt 1380 cttgaagtta tgcagtcatt tccgtcagct aaaccgccac ttggtgtttt cttcgcagct 1440 gtcgccccac gtttacaacc tcgatattac tcgatttctt cttctccaaa gatgtcacca 1500 aacagaattc atgtgacttg tgcattagtt tatgagacaa cacctgcagg acgtattcac 1560 agaggattgt gttcaacatg gatgaagaat gctgtgcctt tgaccgaaag tccagattgt 1620 agtcaggcgt cgatttttgt tagaacgtct aacttccgac ttccggttga cccgaaagtc 1680 ccggtcatca tgatcggtcc cgggactggg ttagcccctt tcagaggttt tcttcaagaa 1740 cggttagctt tgaaggaatc tggaaccgaa ctcgggtcat ctattttctt tttcggatgc 1800 agaaaccgca aagtggattt tatatacgaa gacgaactaa acaactttgt ggagaccggt 1860 gctttatcgg agcttattgt tgcattctcc cgtgaaggaa ccgcaaagga gtatgtgcaa 1920 cataaaatga gccagaaggc ttcagatatc tggaagttgc tttcagaggg agcatattta 1980 tatgtatgtg gtgatgctaa aggcatggct aaagatgtac acagaaccct tcacacaatt 2040 gtacaagaac agggatctct agattcttcc aaggcagaat tgtatgtaaa gaacctacaa 2100 atgtcgggaa gatatcttcg tgatgtttgg taa 2133

<210> 74 <211> 2079 <212> DNA <213> Arabidopsis thaliana

<400> 74 atgacttctg ctttgtatgc ttccgatttg tttaagcagc tcaagtcaat tatggggaca 60 gattcgttat ccgacgatgt tgtacttgtg attgcaacga cgtctttggc actagtagct 120 ggatttgtgg tgttgttatg gaagaaaacg acggcggatc ggagcgggga gctgaagcct 180 ttgatgatcc ctaagtctct tatggctaag gacgaggatg atgatttgga tttgggatcc 240 gggaagacta gagtctctat cttcttcggt acgcagactg gaacagctga gggatttgct 300 aaggcattat ccgaagaaat caaagcgaga tatgaaaaag cagcagtcaa agtcattgac 360 ttggatgact atgctgccga tgatgaccag tatgaagaga Page 75 aattgaagaa ggaaactttg 420

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gcatttttct gtgttgctac ttatggagat ggagagccta ctgacaatgc tgccagattt 480 tacaaatggt ttacggagga aaatgaacgg gatataaagc ttcaacaact agcatatggt 540 gtgtttgctc ttggtaatcg ccaatatgaa cattttaata agatcgggat agttcttgat 600 gaagagttat gtaagaaagg tgcaaagcgt cttattgaag tcggtctagg agatgatgat 660 cagagcattg aggatgattt taatgcctgg aaagaatcac tatggtctga gctagacaag 720 ctcctcaaag acgaggatga taaaagtgtg gcaactcctt atacagctgt tattcctgaa 780 taccgggtgg tgactcatga tcctcggttt acaactcaaa aatcaatgga atcaaatgtg 840 gccaatggaa atactactat tgacattcat catccctgca gagttgatgt tgctgtgcag 900 aaggagcttc acacacatga atctgatcgg tcttgcattc atctcgagtt cgacatatcc 960 aggacgggta ttacatatga aacaggtgac catgtaggtg tatatgctga aaatcatgtt 1020 gaaatagttg aagaagctgg aaaattgctt ggccactctt tagatttagt attttccata 1080 catgctgaca aggaagatgg ctccccattg gaaagcgcag tgccgcctcc tttccctggt 1140 ccatgcacac ttgggactgg tttggcaaga tacgcagacc ttttgaaccc tcctcgaaag 1200 tctgcgttag ttgccttggc ggcctatgcc actgaaccaa gtgaagccga gaaacttaag 1260 cacctgacat cacctgatgg aaaggatgag tactcacaat ggattgttgc aagtcagaga 1320 agtcttttag aggtgatggc tgcttttcca tctgcaaaac ccccactagg tgtatttttt 1380 gctgcaatag ctcctcgtct acaacctcgt tactactcca tctcatcctc gccaagattg 1440 gcgccaagta gagttcatgt tacatccgca ctagtatatg gtccaactcc tactggtaga 1500 atccacaagg gtgtgtgttc tacgtggatg aagaatgcag ttcctgcgga gaaaagtcat 1560 gaatgtagtg gagccccaat ctttattcga gcatctaatt tcaagttacc atccaaccct 1620 tcaactccaa tcgttatggt gggacctggg actgggctgg caccttttag aggttttctg 1680 caggaaagga tggcactaaa agaagatgga gaagaactag gttcatcttt gctcttcttt 1740 gggtgtagaa atcgacagat ggactttata tacgaggatg agctcaataa ttttgttgat 1800 caaggcgtaa tatctgagct catcatggca ttctcccgtg aaggagctca gaaggagtat 1860 gttcaacata agatgatgga gaaggcagca caagtttggg atctaataaa ggaagaagga 1920 tatctctatg tatgcggtga tgctaagggc atggcgaggg acgtccaccg aactctacac 1980 accattgttc aggagcagga aggtgtgagt tcgtcagagg cagaggctat agttaagaaa 2040 cttcaaaccg aaggaagata cctcagagat gtctggtga 2079

<210> 75 <211> 2142 <212> DNA <213> Giberella fujikuroi

<400> 75 atggctgaac tcgacactct ggacatcgtc gtcctcggcg ttatcttcct cggaacggtt 60 gcatacttta caaagggcaa gctatggggt gttaccaagg atccctacgc gaatggcttc 120 gctgccggcg gcgcttctaa gccgggtcgc acgaggaaca tcgtcgaggc aatggaagaa 180

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tccggcaaga actgtgttgt cttctatggt tctcagaccg gtactgctga agattatgct 240 tctcgcctcg ccaaggaggg taagagtcga ttcggactaa acaccatgat tgccgatctt 300 gaggactacg atttcgacaa cctggatacc gttcccagtg acaacattgt catgttcgtt 360 ctcgcaactt atggtgaagg tgagcctacc gataacgcgg tcgacttcta tgaattcatt 420 accggcgagg atgccagctt caatgagggc aatgatcctc cgctgggcaa cctcaactac 480 gttgctttcg gtctcggaaa caacacgtac gagcactaca actctatggt ccgcaatgtt 540 aacaaggctc tcgagaagct tggcgctcac cgcatcggtg aagctggtga gggtgatgat 600 ggtgctggta ccatggaaga ggacttcttg gcctggaagg atcccatgtg ggaagccctc 660 gctaagaaaa tgggactgga agagcgtgaa gcagtctacg agcctatttt tgccattaac 720 gaacgcgacg acctgactcc tgaagccaat gaagtgtatc tcggtgagcc caacaagctg 780 catctcgaag gcaccgccaa gggaccattc aactctcaca acccctacat tgcccctatc 840 gctgaatctt atgagttgtt ctccgccaag gacagaaact gcctccacat ggaaattgac 900 atcagcggtt ctaacctcaa gtacgaaact ggagaccata ttgctatctg gcctaccaac 960 cctggtgagg aggtcaacaa attcctggat attctcgacc tctctggaaa gcagcacagc 1020 gttgtcactg tcaaggctct cgagcctacc gccaaggttc ctttccccaa ccctacaacc 1080 tacgatgcca ttctgcgata ccacctcgag atctgcgctc ctgtttcacg tcaattcgtc 1140 tctactctcg ccgcatttgc tcccaacgat gatatcaagg ctgagatgaa ccgccttggc 1200 agcgataagg attatttcca cgagaagact ggcccgcatt actacaacat tgcccgtttc 1260 cttgccagcg tcagcaaggg cgagaagtgg accaaaatcc cgttctctgc cttcatcgag 1320 ggtctcacca agctccagcc ccgttactac tccatttctt cctcgtctct ggttcagccc 1380 aagaaaatct cgatcactgc cgtcgttgaa tcccagcaga ttcctggccg ggatgatcct 1440 ttccgtggtg ttgctacaaa ctatcttttt gccctaaagc aaaagcagaa cggtgacccc 1500 aaccctgcac cttttggtca gagctacgag cttacaggcc cccgcaataa gtatgatggc 1560 atccacgttc ctgtccatgt tcgtcactcc aacttcaagc tcccctcgga ccccggtaag 1620 cccatcatca tgattggtcc tggtactggt gtcgctccct tccgcggttt cgtgcaggag 1680 cgtgctaagc aagcccgtga tggtgttgag gttggaaaga cactcttgtt ctttggttgc 1740 cgaaagtcaa ccgaggattt catgtaccaa aaggagtggc aggaatacaa ggaggctctt 1800 ggcgataagt ttgaaatgat caccgccttt tctcgagagg gctccaagaa ggtttatgtt 1860 cagcaccgac ttaaggagcg atctaaggag gtcagcgatc tgctctccca gaaggcttat 1920 ttctatgtct gcggtgatgc agcccacatg gcccgcgagg tcaataccgt cttggcacaa 1980 atcattgccg agggacgtgg ggtgtctgag gccaagggcg aggagatcgt gaagaacatg 2040 agatcagcga accaatacca ggtatgtagt gactttgtta ctcttcactg caaagaaacc 2100 acatatgcta actcagaatt acaggaggat gtttggtcat ag 2142

<210> 76

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2018200459 19 Jan 2018 <211> 459 <212> PRT <213> Ipomoea purpurea

<400> 76 Tyr His Met 10 Ala Met Tyr Pro T rp 15 Phe Met 1 Gly Ser Gln Ala 5 Thr Thr Gly Val Gly His Leu Thr Gly Phe Phe Arg Leu Ala Asn Lys Leu Ala 20 25 30 Gly Lys Gly His Arg Ile Ser Phe Leu Ile Pro Lys Asn Thr Gln Ser 35 40 45 Lys Leu Glu Ser Phe Asn Leu His Pro His Leu Ile Ser Phe Val Pro 50 55 60 Ile Val Val Pro Ser Ile Pro Gly Leu Pro Pro Gly Ala Glu Thr Thr 65 70 75 80 Ser Asp Val Pro Phe Pro Ser Thr His Leu Leu Met Glu Ala Met Asp 85 90 95 Lys Thr Gln Asn Asp Ile Gl u Ile Ile Leu Lys Asp Leu Lys Val Asp 100 105 110 Val Val Phe Tyr Asp Phe Thr His T rp Leu Pro Ser Leu Ala Arg Lys 115 120 125 Ile Gly Ile Lys Ser Val Phe Tyr Ser Thr Ile Ser Pro Leu Met His 130 135 140 Gly Tyr Ala Leu Ser Pro Gl u Arg Arg Val Val Gly Lys Gln Leu Thr 145 150 155 160 Glu Ala Asp Met Met Lys Ala Pro Ala Ser Phe Pro Asp Pro Ser Ile 165 170 175 Lys Leu His Ala His Glu Ala Arg Gly Phe Thr Ala Arg Thr Val Met 180 185 190 Lys Phe Gly Gly Asp Ile Thr Phe Phe Asp Arg Ile Phe Thr Ala Val 195 200 205 Ser Glu Ser Asp Gly Leu Ala Tyr Ser Thr cys Arg Glu Ile Glu Gly 210 215 220 Gln Phe cys Asp Tyr Ile Gl u Thr Gln Phe Gln Lys Pro Val Leu Leu 225 230 235 240 Ala Gly Pro Ala Leu Pro Val Pro Ser Lys Ser Thr Met Glu Gln Lys 245 250 255

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T rp Ser Asp Trp 260 Leu Gly Lys Phe Lys 265 Glu Gly Ser Val Ile 270 Tyr cys Ala Phe Gly Ser Glu cys Thr Leu Arg Lys Asp Lys Phe Gln Glu Leu 275 280 285 Leu T rp Gly Leu Glu Leu Thr Gly Met Pro Phe Phe Ala Ala Leu Lys 290 295 300 Pro Pro Phe Glu Thr Glu Ser Val Glu Ala Ala Ile Pro Glu Glu Leu 305 310 315 320 Lys Glu Lys Ile Gln Gly Arg Gly Ile Val His Gly Glu T rp Val Gln 325 330 335 Gln Gln Leu Phe Leu Gln His Pro Ser Val Gly cys Phe Val Ser His 340 345 350 cys Gly T rp Ala Ser Leu Ser Glu Ala Leu Val Asn Asp cys Gln Ile 355 360 365 Val Leu Leu Pro Gln Val Gly Asp Gln Ile Ile Asn Ala Arg Ile Met 370 375 380 Ser Val Ser Leu Lys Val Gly Val Glu Val Glu Lys Gly Glu Glu Asp 385 390 395 400 Gly Val Phe Ser Arg Glu Ser Val cys Lys Ala Val Lys Ala Val Met 405 410 415 Asp Glu Lys Ser Glu Ile Gly Arg Glu Val Arg Gly Asn His Asp Lys 420 425 430 Leu Arg Gly Phe Leu Met Asn Ala Asp Leu Asp Ser Lys Tyr Met Asp 435 440 445 Ser Phe Asn Gln Lys Leu Gl n Asp Leu Leu Gly 450 455

<210> 77 <211> 1380 <212> DNA <213> Ipomoea purpurea <400> 77

atgggttctc aagctacaac ttaccatatg gccatgtatc catggtttgg ggttggacat 60 ttgactggtt tcttccgttt ggcaaacaaa ttagctggca aaggacatag aatctcattt 120 ctaattccta aaaacactca atctaagtta gaatctttca accttcatcc acacttaatc 180 tcttttgtgc ctatcgttgt cccaagtata ccaggcctgc cacctggtgc agagactaca 240 tcagatgttc ctttcccaag tacacatttg ctaatggaag caatggacaa gactcaaaac 300 gatatagaga ttatcctgaa ggatcttaaa gtagatgttg ttttctatga ttttactcac 360

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tggttgcctt ctctggccag aaagattggc attaagagtg tcttttactc caccatttct 420 cctttaatgc atggatatgc tttatcacca gaaagacgtg tagttggtaa gcaattgaca 480 gaggcagata tgatgaaggc cccagcttct ttcccagacc catccattaa gctacatgca 540 catgaagcta ggggttttac agccagaacc gttatgaaat tcggtggtga catcaccttt 600 ttcgatagaa tattcacagc agtttccgaa agtgatggcc tggcctactc tacttgtaga 660 gagatcgagg gacaattctg tgattacatt gaaacacaat tccagaagcc agtcttgtta 720 gccggtccag ctttgccagt cccatccaaa tccactatgg aacaaaagtg gtcagattgg 780 ttggggaaat tcaaggaagg ctccgtcatc tactgtgctt tcgggtctga atgtacattg 840 agaaaggaca aatttcagga acttttatgg ggtttggaat tgacaggaat gcctttcttc 900 gctgctctga agccaccttt tgagactgag tctgttgagg ctgctatccc tgaggaacta 960 aaggaaaaga ttcagggaag aggtatagta catggagaat gggtacaaca acaattgttt 1020 cttcaacacc catctgtcgg gtgcttcgtt tctcactgcg gctgggcaag tttatctgaa 1080 gcccttgtta atgattgtca aatcgtgtta cttccacaag ttggcgatca gattatcaac 1140 gccagaataa tgtcagtatc acttaaagtg ggcgtggaag ttgaaaaggg tgaggaggac 1200 ggtgtctttt caagagaatc tgtgtgcaag gctgttaaag cagtaatgga tgaaaaatct 1260 gaaatcggta gagaagtcag aggtaatcat gataaactga ggggtttctt gatgaatgca 1320 gacttagatt caaagtacat ggattcattc aatcaaaagc tacaagattt gctaggttaa 1380

<210> 78 <211> 438 <212> PRT <213> Bellis perennis

<400> 78 Asp Ser Lys Thr Phe Arg 10 Val Val Met Leu 15 Pro Met 1 Asp Ser Lys Ile 5 T rp Leu Ala Tyr Ser His Ile Ser Ser Phe Leu Val Phe Ala Lys Arg 20 25 30 Leu Thr Asn His Asn Phe Hi s Ile Tyr Ile cys Ser Ser Gln Thr Asn 35 40 45 Met Gln Tyr Leu Lys Asn Asn Leu Thr Ser Gln Tyr Ser Lys Ser Ile 50 55 60 Gln Leu Ile Glu Leu Asn Leu Pro Ser Ser Ser Glu Leu Pro Leu Gln 65 70 75 80 Tyr His Thr Thr His Gly Leu Pro Pro His Leu Thr Lys Thr Leu Ser 85 90 95 Asp Asp Tyr Gln Lys Ser Gly Pro Asp Phe Glu Thr Ile Leu Ile Lys

100 105 110

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Leu Asn Pro 115 His Leu Val Ile Tyr Asp 120 Phe Asn Gln Leu Trp 125 Ala Pro Glu Val Ala Ser Thr Leu His Ile Pro Ser Ile Gln Leu Leu Ser Gly 130 135 140 cys Val Ala Leu Tyr Ala Leu Asp Ala His Leu Tyr Thr Lys Pro Leu 145 150 155 160 Asp Glu Asn Leu Ala Lys Phe Pro Phe Pro Glu Ile Tyr Pro Lys Asn 165 170 175 Arg Asp Ile Pro Lys Gly Gly Ser Lys Tyr Ile Glu Arg Phe Val Asp 180 185 190 cys Met Arg Arg Ser cys Gl u Ile Ile Leu Val Arg Ser Thr Met Glu 195 200 205 Leu Glu Gly Lys Tyr Ile Asp Tyr Leu Ser Lys Thr Leu Gly Lys Lys 210 215 220 Val Leu Pro Val Gly Pro Leu Val Gln Glu Ala Ser Leu Leu Gln Asp 225 230 235 240 Asp His Ile T rp Ile Met Lys T rp Leu Asp Lys Lys Glu Glu Ser Ser 245 250 255 Val Val Phe Val cys Phe Gly Ser Glu Tyr Ile Leu Ser Asp Asn Glu 260 265 270 Ile Glu Asp Ile Ala Tyr Gly Leu Glu Leu Ser Gln Val Ser Phe Val 275 280 285 T rp Ala Ile Arg Ala Lys Thr Ser Ala Leu Asn Gly Phe Ile Asp Arg 290 295 300 Val Gly Asp Lys Gly Leu Val Ile Asp Lys T rp Val Pro Gln Ala Asn 305 310 315 320 Ile Leu Ser His Ser Ser Thr Gly Gly Phe Ile Ser His cys Gly T rp 325 330 335 Ser Ser Thr Met Glu Ser Ile Arg Tyr Gly Val Pro Ile Ile Ala Met 340 345 350 Pro Met Gln Phe Asp Gln Pro Tyr Asn Ala Arg Leu Met Glu Thr Val 355 360 365 Gly Ala Gly Ile Glu Val Gly Arg Asp Gly Glu Gly Arg Leu Lys Arg 370 375 380

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Glu 385 Glu Ile Ala Ala Val 390 Val Arg Lys Val Val 395 Val Glu Asp Ser Gly 400 Glu Ser Ile Arg Glu Lys Ala Lys Glu Leu Gly Glu Ile Met Lys Lys 405 410 415 Asn Met Glu Ala Glu Val Asp Gly Ile Val Ile Glu Asn Leu Val Lys 420 425 430

Leu Cys Glu Met Asn Asn 435 <210> 79 <211> 1317 <212> DNA <213> Bellis perennis <400> 79

atggattcta aaatcgattc aaagacattc agagtcgtta tgttgccttg gcttgcatac 60 tcacacattt catcattcct agtgtttgcc aagagactaa caaatcataa cttccacatc 120 tacatttgtt cctctcaaac aaatatgcaa tacctgaaaa acaacttgac gtctcagtat 180 tcaaaatcta tacaactgat tgagttgaat cttccatcta gttccgaatt gcctctgcag 240 tatcatacta ctcacggact accaccacac cttacgaaaa cattgtctga tgattatcaa 300 aagtccggac ctgactttga aaccattttg atcaaattga acccacatct ggtaatctac 360 gactttaatc aactttgggc tccagaggtt gctagtacac ttcatattcc atccatacag 420 ttactgtctg gttgcgtcgc cttatatgcc ttagacgccc atctgtacac aaagccacta 480 gacgaaaact tggctaagtt tcctttccca gaaatctatc ctaaaaacag agatattcct 540 aagggaggta gtaaatacat cgaaaggttc gtagactgta tgagaagatc ttgtgaaatc 600 atattagtca gaagtaccat ggaacttgaa ggaaaataca ttgattactt gtctaagaca 660 ttagggaaaa aggtgttgcc agtagggcct ctggtgcaag aggcttcttt gttgcaagat 720 gatcatatat ggattatgaa gtggttagac aaaaaggagg agtcatccgt cgtgtttgtt 780 tgttttggtt ctgagtacat cttatcagac aacgaaatag aagatattgc ttatggccta 840 gagttgtccc aagtaagttt cgtttgggca ataagagcta agacttctgc cttaaatggc 900 ttcattgata gagtgggtga taaaggctta gtcatcgata aatgggttcc acaggctaac 960 atcttatctc actcttctac tggtggattc attagtcatt gcggttggtc atcaacaatg 1020 gaatctatta gatatggggt tcctattatc gccatgccaa tgcaattcga tcaaccttac 1080 aatgctaggt tgatggaaac tgttggtgca ggtatcgaag ttggcagaga tggcgaaggt 1140 agattgaaaa gagaagagat tgctgccgtg gttagaaagg tcgttgttga agattctggg 1200 gaatccataa gggagaaggc aaaggaattg ggagaaatca tgaaaaaaaa catggaggcc 1260 gaagtagatg gtatagtgat tgaaaatcta gttaagctat gtgagatgaa caattaa 1317

<210> 80 <211> 2490

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2018200459 19 Jan 2018 <212> DNA <213> Zea mays <400> 80

atggttttgt cttcttcttg tactacagta ccacacttat cttcattagc tgtcgtgcaa 60 cttggtcctt ggagcagtag gattaaaaag aaaaccgata ctgttgcagt accagccgct 120 gcaggaaggt ggagaagggc cttggctaga gcacagcaca catcagaatc cgcagctgtc 180 gcaaagggca gcagtttgac ccctatagtg agaactgacg ctgagtcaag gagaacaaga 240 tggccaaccg atgacgatga cgccgaacct ttagtggatg agatcagggc aatgcttact 300 tccatgtctg atggtgacat ttccgtgagc gcatacgata cagcctgggt cggattggtt 360 ccaagattag acggcggtga aggtcctcaa tttccagcag ctgtgagatg gataagaaat 420 aaccagttgc ctgacggaag ttggggcgat gccgcattat tctctgccta tgacaggctt 480 atcaataccc ttgcctgcgt tgtaactttg acaaggtggt ccctagaacc agagatgaga 540 ggtagaggac tatctttttt gggtaggaac atgtggaaat tagcaactga agatgaagag 600 tcaatgccta ttggcttcga attagcattt ccatctttga tagagcttgc taagagccta 660 ggtgtccatg acttccctta tgatcaccag gccctacaag gaatctactc ttcaagagag 720 atcaaaatga agaggattcc aaaagaagtg atgcataccg ttccaacatc aatattgcac 780 agtttggagg gtatgcctgg cctagattgg gctaaactac ttaaactaca gagcagcgac 840 ggaagttttt tgttctcacc agctgccact gcatatgctt taatgaatac cggagatgac 900 aggtgtttta gctacatcga tagaacagta aagaaattca acggcggcgt ccctaatgtt 960 tatccagtgg atctatttga acatatttgg gccgttgata gacttgaaag attaggaatc 1020 tccaggtact tccaaaagga gatcgaacaa tgcatggatt atgtaaacag gcattggact 1080 gaggacggta tttgttgggc aaggaactct gatgtcaaag aggtggacga cacagctatg 1140 gcctttagac ttcttaggtt gcacggctac agcgtcagtc ctgatgtgtt taaaaacttc 1200 gaaaaggacg gtgaattttt cgcatttgtc ggacagtcta atcaagctgt taccggtatg 1260 tacaacttaa acagagcaag ccagatatcc ttcccaggcg aggatgtgct tcatagagct 1320 ggtgccttct catatgagtt cttgaggaga aaagaagcag agggagcttt gagggacaag 1380 tggatcattt ctaaagatct acctggtgaa gttgtgtata ctttggattt tccatggtac 1440 ggcaacttac ctagagtcga ggccagagac tacctagagc aatacggagg tggtgatgac 1500 gtttggattg gcaagacatt gtataggatg ccacttgtaa acaatgatgt atatttggaa 1560 ttggcaagaa tggatttcaa ccactgccag gctttgcatc agttagagtg gcaaggacta 1620 aaaagatggt atactgaaaa taggttgatg gactttggtg tcgcccaaga agatgccctt 1680 agagcttatt ttcttgcagc cgcatctgtt tacgagcctt gtagagctgc cgagaggctt 1740 gcatgggcta gagccgcaat actagctaac gccgtgagca cccacttaag aaatagccca 1800 tcattcagag aaaggttaga gcattctctt aggtgtagac ctagtgaaga gacagatggc 1860 tcctggttta actcctcaag tggctctgat gcagttttag taaaggctgt cttaagactt 1920 actgattcat tagccaggga agcacagcca atccatggag gtgacccaga agatattata 1980

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cacaagttgt taagatctgc ttgggccgag tgggttaggg aaaaggcaga cgctgccgat 2040 agcgtgtgca atggtagttc tgcagtagaa caagagggat caagaatggt ccatgataaa 2100 cagacctgtc tattattggc tagaatgatc gaaatttctg ccggtagggc agctggtgaa 2160 gcagccagtg aggacggcga tagaagaata attcaattaa caggctccat ctgcgacagt 2220 cttaagcaaa aaatgctagt ttcacaggac cctgaaaaaa atgaagagat gatgtctcac 2280 gtggatgacg aattgaagtt gaggattaga gagttcgttc aatatttgct tagactaggt 2340 gaaaaaaaga ctggatctag cgaaaccagg caaacatttt taagtatagt gaaatcatgt 2400 tactatgctg ctcattgccc acctcatgtc gttgatagac acattagtag agtgattttc 2460 gagccagtaa gtgccgcaaa gtaaccgcgg 2490

<210> 81 <211> 827 <212> PRT <213> Zea mays

<400> 81 Met 1 Val Leu Ser Ser Ser cys 5 Thr Thr Val 10 Pro His Leu Ser Ser 15 Leu Ala Val Val Gln Leu Gly Pro T rp Ser Ser Arg Ile Lys Lys Lys Thr 20 25 30 Asp Thr Val Ala Val Pro Ala Ala Ala Gly Arg T rp Arg Arg Ala Leu 35 40 45 Ala Arg Ala Gln His Thr Ser Glu Ser Ala Ala Val Ala Lys Gly Ser 50 55 60 Ser Leu Thr Pro Ile Val Arg Thr Asp Ala Glu Ser Arg Arg Thr Arg 65 70 75 80 T rp Pro Thr Asp Asp Asp Asp Ala Glu Pro Leu Val Asp Glu Ile Arg 85 90 95 Ala Met Leu Thr Ser Met Ser Asp Gly Asp Ile Ser Val Ser Ala Tyr 100 105 110 Asp Thr Ala T rp Val Gly Leu Val Pro Arg Leu Asp Gly Gly Glu Gly 115 120 125 Pro Gln Phe Pro Ala Ala Val Arg T rp Ile Arg Asn Asn Gln Leu Pro 130 135 140 Asp Gly Ser T rp Gly Asp Ala Ala Leu Phe Ser Ala Tyr Asp Arg Leu 145 150 155 160 Ile Asn Thr Leu Ala cys Val Val Thr Leu Thr Arg T rp Ser Leu Glu

165 170 175

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2018200459 19 Jan 2018

Pro Glu Met Arg 180 Gly Arg Gly Leu Ser 185 Phe Leu Gly Arg Asn 190 Met T rp Lys Leu Ala Thr Glu Asp Gl u Glu Ser Met Pro Ile Gly Phe Glu Leu 195 200 205 Ala Phe Pro Ser Leu Ile Gl u Leu Ala Lys Ser Leu Gly Val His Asp 210 215 220 Phe Pro Tyr Asp His Gln Ala Leu Gln Gly Ile Tyr Ser Ser Arg Glu 225 230 235 240 Ile Lys Met Lys Arg Ile Pro Lys Glu Val Met His Thr Val Pro Thr 245 250 255 Ser Ile Leu His Ser Leu Gl u Gly Met Pro Gly Leu Asp T rp Ala Lys 260 265 270 Leu Leu Lys Leu Gln Ser Ser Asp Gly Ser Phe Leu Phe Ser Pro Ala 275 280 285 Ala Thr Ala Tyr Ala Leu Met Asn Thr Gly Asp Asp Arg cys Phe Ser 290 295 300 Tyr Ile Asp Arg Thr Val Lys Lys Phe Asn Gly Gly Val Pro Asn Val 305 310 315 320 Tyr Pro Val Asp Leu Phe Gl u His Ile T rp Ala Val Asp Arg Leu Glu 325 330 335 Arg Leu Gly Ile Ser Arg Tyr Phe Gln Lys Glu Ile Glu Gln cys Met 340 345 350 Asp Tyr Val Asn Arg His Trp Thr Glu Asp Gly Ile cys T rp Ala Arg 355 360 365 Asn Ser Asp Val Lys Glu Val Asp Asp Thr Ala Met Ala Phe Arg Leu 370 375 380 Leu Arg Leu His Gly Tyr Ser Val Ser Pro Asp Val Phe Lys Asn Phe 385 390 395 400 Glu Lys Asp Gly Glu Phe Phe Ala Phe Val Gly Gln Ser Asn Gln Ala 405 410 415 Val Thr Gly Met Tyr Asn Leu Asn Arg Ala Ser Gln Ile Ser Phe Pro 420 425 430 Gly Glu Asp Val Leu His Arg Ala Gly Ala Phe Ser Tyr Glu Phe Leu 435 440 445

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Arg Arg Lys Glu Ala Glu Gly 455 Ala Leu Arg Asp Lys 460 T rp Ile Ile Ser 450 Lys Asp Leu Pro Gly Glu Val Val Tyr Thr Leu Asp Phe Pro T rp Tyr 465 470 475 480 Gly Asn Leu Pro Arg Val Gl u Ala Arg Asp Tyr Leu Glu Gln Tyr Gly 485 490 495 Gly Gly Asp Asp Val T rp Ile Gly Lys Thr Leu Tyr Arg Met Pro Leu 500 505 510 Val Asn Asn Asp Val Tyr Leu Glu Leu Ala Arg Met Asp Phe Asn His 515 520 525 cys Gln Ala Leu His Gln Leu Glu T rp Gln Gly Leu Lys Arg T rp Tyr 530 535 540 Thr Glu Asn Arg Leu Met Asp Phe Gly Val Ala Gln Glu Asp Ala Leu 545 550 555 560 Arg Ala Tyr Phe Leu Ala Ala Ala Ser Val Tyr Glu Pro cys Arg Ala 565 570 575 Ala Glu Arg Leu Ala T rp Ala Arg Ala Ala Ile Leu Ala Asn Ala Val 580 585 590 Ser Thr His Leu Arg Asn Ser Pro Ser Phe Arg Glu Arg Leu Glu His 595 600 605 Ser Leu Arg cys Arg Pro Ser Glu Glu Thr Asp Gly Ser T rp Phe Asn 610 615 620 Ser Ser Ser Gly Ser Asp Ala Val Leu Val Lys Ala Val Leu Arg Leu 625 630 635 640 Thr Asp Ser Leu Ala Arg Gl u Ala Gln Pro Ile His Gly Gly Asp Pro 645 650 655 Glu Asp Ile Ile His Lys Leu Leu Arg Ser Ala T rp Ala Glu T rp Val 660 665 670 Arg Glu Lys Ala Asp Ala Ala Asp Ser Val cys Asn Gly Ser Ser Ala 675 680 685 Val Glu Gln Glu Gly Ser Arg Met Val His Asp Lys Gln Thr cys Leu 690 695 700 Leu Leu Ala Arg Met Ile Gl u Ile Ser Ala Gly Arg Ala Ala Gly Glu 705 710 715 720 Ala Ala Ser Glu Asp Gly Asp Arg Arg Ile Ile Gln Leu Thr Gly Ser

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2018200459 19 Jan 2018

725

730

735

Ile cys Asp Ser 740 Leu Lys Gln Lys Met 745 Leu Val Ser Gln Asp 750 Pro Glu Lys Asn Glu Glu Met Met Ser His Val Asp Asp Glu Leu Lys Leu Arg 755 760 765 Ile Arg Glu Phe Val Gln Tyr Leu Leu Arg Leu Gly Glu Lys Lys Thr 770 775 780 Gly Ser Ser Glu Thr Arg Gl n Thr Phe Leu Ser Ile Val Lys Ser cys 785 790 795 800 Tyr Tyr Ala Ala His cys Pro Pro His Val Val Asp Arg His Ile Ser 805 810 815 Arg Val Ile Phe Glu Pro Val Ser Ala Ala Lys 820 825

<210> 82 <211> 1461 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 82 actagtaaaa tggatgcaat ggcaactact gagaaaaagc ctcatgtgat cttcattcca 60 tttcctgcac aatctcacat aaaggcaatg ctaaagttag cacaactatt acaccataag 120 ggattacaga taactttcgt gaataccgac ttcatccata atcaatttct ggaatctagt 180 ggccctcatt gtttggacgg agccccaggg tttagattcg aaacaattcc tgacggtgtt 240 tcacattccc cagaggcctc catcccaata agagagagtt tactgaggtc aatagaaacc 300 aactttttgg atcgtttcat tgacttggtc acaaaacttc cagacccacc aacttgcata 360 atctctgatg gctttctgtc agtgtttact atcgacgctg ccaaaaagtt gggtatccca 420 gttatgatgt actggactct tgctgcatgc ggtttcatgg gtttctatca catccattct 480 cttatcgaaa agggttttgc tccactgaaa gatgcatcat acttaaccaa cggctacctg 540 gatactgtta ttgactgggt accaggtatg gaaggtataa gacttaaaga ttttcctttg 600 gattggtcta cagaccttaa tgataaagta ttgatgttta ctacagaagc tccacaaaga 660 tctcataagg tttcacatca tatctttcac acctttgatg aattggaacc atcaatcatc 720 aaaaccttgt ctctaagata caatcatatc tacactattg gtccattaca attacttcta 780 gatcaaattc ctgaagagaa aaagcaaact ggtattacat ccttacacgg ctactcttta 840 gtgaaagagg aaccagaatg ttttcaatgg ctacaaagta aagagcctaa ttctgtggtc 900 tacgtcaact tcggaagtac aacagtcatg tccttggaag atatgactga atttggttgg 960

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2018200459 19 Jan 2018 ggccttgcta attcaaatca ttactttcta tggattatca ggtccaattt ggtaataggg 1020 gaaaacgccg tattacctcc agaattggag gaacacatca aaaagagagg tttcattgct 1080 tcctggtgtt ctcaggaaaa ggtattgaaa catccttctg ttggtggttt ccttactcat 1140 tgcggttggg gctctacaat cgaatcacta agtgcaggag ttccaatgat ttgttggcca 1200 tattcatggg accaacttac aaattgtagg tatatctgta aagagtggga agttggatta 1260 gaaatgggaa caaaggttaa acgtgatgaa gtgaaaagat tggttcagga gttgatgggg 1320 gaaggtggcc acaagatgag aaacaaggcc aaagattgga aggaaaaagc cagaattgct 1380 attgctccta acgggtcatc ctctctaaac attgataaga tggtcaaaga gattacagtc 1440 ttagccagaa actaagtcga c 1461 <210> 83 <211> 1398 <212> DNA <213> Artificial Sequence

<220> <223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 83 actagtaaaa tggcagagca acaaaagatc aaaaagtcac ctcacgtctt acttattcca 60 tttcctctgc aaggacatat caacccattc atacaatttg ggaaaagatt gattagtaag 120 ggtgtaaaga caacactggt aaccactatc cacactttga attctactct gaaccactca 180 aatactacta ctacaagtat agaaattcaa gctatatcag acggatgcga tgagggtggc 240 tttatgtctg ccggtgaatc ttacttggaa acattcaagc aagtgggatc caagtctctg 300 gccgatctaa tcaaaaagtt acagagtgaa ggcaccacaa ttgacgccat aatctacgat 360 tctatgacag agtgggtttt agacgttgct atcgaatttg gtattgatgg aggttccttt 420 ttcacacaag catgtgttgt gaattctcta tactaccatg tgcataaagg gttaatctct 480 ttaccattgg gtgaaactgt ttcagttcca ggttttccag tgttacaacg ttgggaaacc 540 ccattgatct tacaaaatca tgaacaaata caatcacctt ggtcccagat gttgtttggt 600 caattcgcta acatcgatca agcaagatgg gtctttacta attcattcta taagttagag 660 gaagaggtaa ttgaatggac taggaagatc tggaatttga aagtcattgg tccaacattg 720 ccatcaatgt atttggacaa aagacttgat gatgataaag ataatggttt caatttgtac 780 aaggctaatc atcacgaatg tatgaattgg ctggatgaca aaccaaagga atcagttgta 840 tatgttgctt tcggctctct tgttaaacat ggtccagaac aagttgagga gattacaaga 900 gcacttatag actctgacgt aaactttttg tgggtcatta agcacaaaga ggaggggaaa 960 ctgccagaaa acctttctga agtgataaag accggaaaag gtctaatcgt tgcttggtgt 1020 aaacaattgg atgttttagc tcatgaatct gtaggctgtt ttgtaacaca ttgcggattc 1080 aactctacac tagaagccat ttccttaggc gtacctgtcg ttgcaatgcc tcagttctcc 1140 gatcagacaa ccaacgctaa acttttggac gaaatactag gggtgggtgt cagagttaaa 1200

Page 88 gcagacgaga atggtatcgt cagaagaggg aacctagctt catgtatcaa aatgatcatg 1260 gaagaggaaa gaggagttat cataaggaaa aacgcagtta agtggaagga tcttgcaaag 1320 gttgccgtcc atgaaggcgg ctcttcagat aatgatattg ttgaatttgt gtccgaacta 1380 atcaaagcct aagtcgac 1398

2018200459 19 Jan 2018 <210> 84 <211> 1437 <212> DNA <213> Artificial Sequence

<220> <223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 84 actagtaaaa tggctacatc tgattctatt gttgatgaca ggaagcagtt gcatgtggct 60 actttccctt ggcttgcttt cggtcatata ctgccttacc tacaactatc aaaactgata 120 gctgaaaaag gacataaagt gtcattcctt tcaacaacta gaaacattca aagattatct 180 tcccacatat caccattgat taacgtcgtt caattgacac ttccaagagt acaggaatta 240 ccagaagatg ctgaagctac aacagatgtg catcctgaag atatccctta cttgaaaaag 300 gcatccgatg gattacagcc tgaggtcact agattccttg agcaacacag tccagattgg 360 atcatatacg actacactca ctattggttg ccttcaattg cagcatcact aggcatttct 420 agggcacatt tcagtgtaac cacaccttgg gccattgctt acatgggtcc atccgctgat 480 gctatgatta acggcagtga tggtagaact accgttgaag atttgacaac cccaccaaag 540 tggtttccat ttccaactaa agtctgttgg agaaaacacg acttagcaag actggttcca 600 tacaaggcac caggaatctc agacggctat agaatgggtt tagtccttaa agggtctgac 660 tgcctattgt ctaagtgtta ccatgagttt gggacacaat ggctaccact tttggaaaca 720 ttacaccaag ttcctgtcgt accagttggt ctattacctc cagaaatccc tggtgatgag 780 aaggacgaga cttgggtttc aatcaaaaag tggttagacg ggaagcaaaa aggctcagtg 840 gtatatgtgg cactgggttc cgaagtttta gtatctcaaa cagaagttgt ggaacttgcc 900 ttaggtttgg aactatctgg attgccattt gtctgggcct acagaaaacc aaaaggccct 960 gcaaagtccg attcagttga attgccagac ggctttgtcg agagaactag agatagaggg 1020 ttggtatgga cttcatgggc tccacaattg agaatcctga gtcacgaatc tgtgtgcggt 1080 ttcctaacac attgtggttc tggttctata gttgaaggac tgatgtttgg tcatccactt 1140 atcatgttgc caatctttgg tgaccagcct ttgaatgcac gtctgttaga agataaacaa 1200 gttggaattg aaatcccacg taatgaggaa gatggatgtt taaccaagga gtctgtggcc 1260 agatcattac gttccgttgt cgttgaaaag gaaggcgaaa tctacaaggc caatgcccgt 1320 gaactttcaa agatctacaa tgacacaaaa gtagagaagg aatatgtttc tcaatttgta 1380 gattacctag agaaaaacgc tagagccgta gctattgatc atgaatccta agtcgac 1437

<210> 85

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2018200459 19 Jan 2018 <211> 1392 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 85

actagtaaaa tggaaaacaa gaccgaaaca acagttagac gtaggcgtag aatcattctg 60 tttccagtac cttttcaagg gcacatcaat ccaatactac aactagccaa cgttttgtac 120 tctaaaggtt tttctattac aatctttcac accaatttca acaaaccaaa aacatccaat 180 tacccacatt tcacattcag attcatactt gataatgatc cacaagatga acgtatttca 240 aacttaccta cccacggtcc tttagctgga atgagaattc caatcatcaa tgaacatggt 300 gccgatgagc ttagaagaga attagagtta cttatgttgg catccgaaga ggacgaggaa 360 gtctcttgtc tgattactga cgctctatgg tactttgccc aatctgtggc tgatagtttg 420 aatttgagga gattggtact aatgacatcc agtctgttta actttcacgc tcatgttagt 480 ttaccacaat ttgacgaatt gggatacttg gaccctgatg acaagactag gttagaggaa 540 caggcctctg gttttcctat gttgaaagtc aaagatatca agtctgccta ttctaattgg 600 caaatcttga aagagatctt aggaaagatg atcaaacaga caaaggcttc atctggagtg 660 atttggaaca gtttcaaaga gttagaagag tctgaattgg agactgtaat cagagaaatt 720 ccagcacctt cattcctgat accattacca aaacatttga ctgcttcctc ttcctctttg 780 ttggatcatg acagaacagt ttttcaatgg ttggaccaac aaccacctag ttctgttttg 840 tacgtgtcat ttggtagtac ttctgaagtc gatgaaaagg acttccttga aatcgcaaga 900 ggcttagtcg atagtaagca gtcattcctt tgggtcgtgc gtccaggttt cgtgaaaggc 960 tcaacatggg tcgaaccact tccagatggt tttctaggcg aaagaggtag aatagtcaaa 1020 tgggttcctc aacaggaagt tttagctcat ggcgctattg gggcattctg gactcattcc 1080 ggatggaatt caactttaga atcagtatgc gaaggggtac ctatgatctt ttcagatttt 1140 ggtcttgatc aaccactgaa cgcaagatac atgtctgatg ttttgaaagt gggtgtatat 1200 ctagaaaatg gctgggaaag gggtgaaata gctaatgcaa taagacgtgt tatggttgat 1260 gaagaggggg agtatatcag acaaaacgca agagtgctga agcaaaaggc cgacgtttct 1320 ctaatgaagg gaggctcttc atacgaatcc ttagaatctc ttgtttccta catttcatca 1380 ctgtaagtcg ac 1392

<210> 86 <211> 12 <212> PRT <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic pepti de <400> 86

Thr Ser Phe Ala Glu Tyr Trp Asn Leu Leu Ser Pro Page 90

2018200459 19 Jan 2018 <210> 87 <211> 1602 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 87 atgaccagct ttgccgagta ttggaatctg ttaagtccca cttcttttgc agaatattgg 60 aaccttctat caccgacgag tttcgcggag tactggaatt tgttttctcc aacatcgttc 120 gctgaatact ggaacttact cagccctgct agtaaaatgg atgcaatggc aactactgag 180 aaaaagcctc atgtgatctt cattccattt cctgcacaat ctcacataaa ggcaatgcta 240 aagttagcac aactattaca ccataaggga ttacagataa ctttcgtgaa taccgacttc 300 atccataatc aatttctgga atctagtggc cctcattgtt tggacggagc cccagggttt 360 agattcgaaa caattcctga cggtgtttca cattccccag aggcctccat cccaataaga 420 gagagtttac tgaggtcaat agaaaccaac tttttggatc gtttcattga cttggtcaca 480 aaacttccag acccaccaac ttgcataatc tctgatggct ttctgtcagt gtttactatc 540 gacgctgcca aaaagttggg tatcccagtt atgatgtact ggactcttgc tgcatgcggt 600 ttcatgggtt tctatcacat ccattctctt atcgaaaagg gttttgctcc actgaaagat 660 gcatcatact taaccaacgg ctacctggat actgttattg actgggtacc aggtatggaa 720 ggtataagac ttaaagattt tcctttggat tggtctacag accttaatga taaagtattg 780 atgtttacta cagaagctcc acaaagatct cataaggttt cacatcatat ctttcacacc 840 tttgatgaat tggaaccatc aatcatcaaa accttgtctc taagatacaa tcatatctac 900 actattggtc cattacaatt acttctagat caaattcctg aagagaaaaa gcaaactggt 960 attacatcct tacacggcta ctctttagtg aaagaggaac cagaatgttt tcaatggcta 1020 caaagtaaag agcctaattc tgtggtctac gtcaacttcg gaagtacaac agtcatgtcc 1080 ttggaagata tgactgaatt tggttggggc cttgctaatt caaatcatta ctttctatgg 1140 attatcaggt ccaatttggt aataggggaa aacgccgtat tacctccaga attggaggaa 1200 cacatcaaaa agagaggttt cattgcttcc tggtgttctc aggaaaaggt attgaaacat 1260 ccttctgttg gtggtttcct tactcattgc ggttggggct ctacaatcga atcactaagt 1320 gcaggagttc caatgatttg ttggccatat tcatgggacc aacttacaaa ttgtaggtat 1380 atctgtaaag agtgggaagt tggattagaa atgggaacaa aggttaaacg tgatgaagtg 1440 aaaagattgg ttcaggagtt gatgggggaa ggtggccaca agatgagaaa caaggccaaa 1500 gattggaagg aaaaagccag aattgctatt gctcctaacg ggtcatcctc tctaaacatt 1560 gataagatgg tcaaagagat tacagtctta gccagaaact aa 1602

<210> 88

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2018200459 19 Jan 2018 <211> 533 <212> PRT <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polypepti de

<400> 88 Leu 10 Leu Ser Pro Thr Ser 15 Phe Met 1 Thr Ser Phe Ala Glu 5 Tyr Trp Asn Ala Glu Tyr T rp Asn Leu Leu Ser Pro Thr Ser Phe Ala Glu Tyr T rp 20 25 30 Asn Leu Phe Ser Pro Thr Ser Phe Ala Glu Tyr T rp Asn Leu Leu Ser 35 40 45 Pro Ala Ser Lys Met Asp Ala Met Ala Thr Thr Glu Lys Lys Pro His 50 55 60 Val Ile Phe Ile Pro Phe Pro Ala Gln Ser His Ile Lys Ala Met Leu 65 70 75 80 Lys Leu Ala Gln Leu Leu His His Lys Gly Leu Gln Ile Thr Phe Val 85 90 95 Asn Thr Asp Phe Ile His Asn Gln Phe Leu Glu Ser Ser Gly Pro His 100 105 110 cys Leu Asp Gly Ala Pro Gly Phe Arg Phe Glu Thr Ile Pro Asp Gly 115 120 125 Val Ser His Ser Pro Glu Ala Ser Ile Pro Ile Arg Glu Ser Leu Leu 130 135 140 Arg Ser Ile Glu Thr Asn Phe Leu Asp Arg Phe Ile Asp Leu Val Thr 145 150 155 160 Lys Leu Pro Asp Pro Pro Thr cys Ile Ile Ser Asp Gly Phe Leu Ser 165 170 175 Val Phe Thr Ile Asp Ala Ala Lys Lys Leu Gly Ile Pro Val Met Met 180 185 190 Tyr T rp Thr Leu Ala Ala cys Gly Phe Met Gly Phe Tyr His Ile His 195 200 205 Ser Leu Ile Glu Lys Gly Phe Ala Pro Leu Lys Asp Ala Ser Tyr Leu 210 215 220 Thr Asn Gly Tyr Leu Asp Thr Val Ile Asp T rp Val Pro Gly Met Glu 225 230 235 240

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2018200459 19 Jan 2018

Gly Ile Arg Leu Lys 245 Asp Phe Pro Leu Asp Trp Ser Thr Asp Leu Asn 250 255 Asp Lys Val Leu Met Phe Thr Thr Glu Ala Pro Gln Arg Ser His Lys 260 265 270 Val Ser His His Ile Phe His Thr Phe Asp Glu Leu Glu Pro Ser Ile 275 280 285 Ile Lys Thr Leu Ser Leu Arg Tyr Asn His Ile Tyr Thr Ile Gly Pro 290 295 300 Leu Gln Leu Leu Leu Asp Gl n Ile Pro Glu Glu Lys Lys Gln Thr Gly 305 310 315 320 Ile Thr Ser Leu His Gly Tyr Ser Leu Val Lys Glu Glu Pro Glu cys 325 330 335 Phe Gln T rp Leu Gln Ser Lys Glu Pro Asn Ser Val Val Tyr Val Asn 340 345 350 Phe Gly Ser Thr Thr Val Met Ser Leu Glu Asp Met Thr Glu Phe Gly 355 360 365 T rp Gly Leu Ala Asn Ser Asn His Tyr Phe Leu T rp Ile Ile Arg Ser 370 375 380 Asn Leu Val Ile Gly Glu Asn Ala Val Leu Pro Pro Glu Leu Glu Glu 385 390 395 400 His Ile Lys Lys Arg Gly Phe Ile Ala Ser T rp cys Ser Gln Glu Lys 405 410 415 Val Leu Lys His Pro Ser Val Gly Gly Phe Leu Thr His cys Gly T rp 420 425 430 Gly Ser Thr Ile Glu Ser Leu Ser Ala Gly Val Pro Met Ile cys T rp 435 440 445 Pro Tyr Ser T rp Asp Gln Leu Thr Asn cys Arg Tyr Ile cys Lys Glu 450 455 460 T rp Glu Val Gly Leu Glu Met Gly Thr Lys Val Lys Arg Asp Glu Val 465 470 475 480 Lys Arg Leu Val Gln Glu Leu Met Gly Glu Gly Gly His Lys Met Arg 485 490 495 Asn Lys Ala Lys Asp T rp Lys Glu Lys Ala Arg Ile Ala Ile Ala Pro 500 505 510 Asn Gly Ser Ser Ser Leu Asn Ile Asp Lys Met Val Lys Glu Ile Thr Page 93

2018200459 19 Jan 2018

515

520

525

Val Leu Ala Arg Asn 530 <210> 89 <211> 1893 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 89 atgtgcaata ccaacatgtc tgtacctact gatggtgctg taaccacctc acagattcca 60 gcttcggaac aagagaccct ggttagacca aagccattgc ttttgaagtt attaaagtct 120 gttggtgcac aaaaagacac ttatactatg aaagaggttc ttttttatct tggccagtat 180 attatgacta aacgattata tgatgagaag caacaacata ttgtatattg ttcaaatgat 240 cttctaggag atttgtttgg cgtgccaagc ttctctgtga aagagcacag gaaaatatat 300 accatgatct acaggaactt ggtagtagtc aatcagcagg aatcatcgga ctcaggtaca 360 tctgtgagtg agaacaggtg tcaccttgaa ggtgggagtg atcaaaagga ccttgtacaa 420 gagcttcagg aagagaaacc ttcatcttca catttggttt ctagaccatc taccggtggt 480 agcggatcct ctggaggcag tgctagtaaa atggcagagc aacaaaagat caaaaagtca 540 cctcacgtct tacttattcc atttcctctg caaggacata tcaacccatt catacaattt 600 gggaaaagat tgattagtaa gggtgtaaag acaacactgg taaccactat ccacactttg 660 aattctactc tgaaccactc aaatactact actacaagta tagaaattca agctatatca 720 gacggatgcg atgagggtgg ctttatgtct gccggtgaat cttacttgga aacattcaag 780 caagtgggat ccaagtctct ggccgatcta atcaaaaagt tacagagtga aggcaccaca 840 attgacgcca taatctacga ttctatgaca gagtgggttt tagacgttgc tatcgaattt 900 ggtattgatg gaggttcctt tttcacacaa gcatgtgttg tgaattctct atactaccat 960 gtgcataaag ggttaatctc tttaccattg ggtgaaactg tttcagttcc aggttttcca 1020 gtgttacaac gttgggaaac cccattgatc ttacaaaatc atgaacaaat acaatcacct 1080 tggtcccaga tgttgtttgg tcaattcgct aacatcgatc aagcaagatg ggtctttact 1140 aattcattct ataagttaga ggaagaggta attgaatgga ctaggaagat ctggaatttg 1200 aaagtcattg gtccaacatt gccatcaatg tatttggaca aaagacttga tgatgataaa 1260 gataatggtt tcaatttgta caaggctaat catcacgaat gtatgaattg gctggatgac 1320 aaaccaaagg aatcagttgt atatgttgct ttcggctctc ttgttaaaca tggtccagaa 1380 caagttgagg agattacaag agcacttata gactctgacg taaacttttt gtgggtcatt 1440 aagcacaaag aggaggggaa actgccagaa aacctttctg aagtgataaa gaccggaaaa 1500 ggtctaatcg ttgcttggtg taaacaattg gatgttttag ctcatgaatc tgtaggctgt 1560

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2018200459 19 Jan 2018 tttgtaacac attgcggatt caactctaca ctagaagcca tttccttagg cgtacctgtc gttgcaatgc ctcagttctc cgatcagaca accaacgcta aacttttgga cgaaatacta ggggtgggtg tcagagttaa agcagacgag aatggtatcg tcagaagagg gaacctagct tcatgtatca aaatgatcat ggaagaggaa agaggagtta tcataaggaa aaacgcagtt aagtggaagg atcttgcaaa ggttgccgtc catgaaggcg gctcttcaga taatgatatt gttgaatttg tgtccgaact aatcaaagcc taa <210> 90 <211> 630 <212> PRT <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polypepti de

<400> 90 Met 1 cys Asn Thr Asn 5 Met Ser Val Pro Thr 10 Asp Gly Ala Val Thr 15 Thr Ser Gln Ile Pro Ala Ser Gl u Gln Glu Thr Leu Val Arg Pro Lys Pro 20 25 30 Leu Leu Leu Lys Leu Leu Lys Ser Val Gly Ala Gln Lys Asp Thr Tyr 35 40 45 Thr Met Lys Glu Val Leu Phe Tyr Leu Gly Gln Tyr Ile Met Thr Lys 50 55 60 Arg Leu Tyr Asp Glu Lys Gl n Gln His Ile Val Tyr cys Ser Asn Asp 65 70 75 80 Leu Leu Gly Asp Leu Phe Gly Val Pro Ser Phe Ser Val Lys Glu His 85 90 95 Arg Lys Ile Tyr Thr Met Ile Tyr Arg Asn Leu Val Val Val Asn Gln 100 105 110 Gln Glu Ser Ser Asp Ser Gly Thr Ser Val Ser Glu Asn Arg cys His 115 120 125 Leu Glu Gly Gly Ser Asp Gl n Lys Asp Leu Val Gln Glu Leu Gln Glu 130 135 140 Glu Lys Pro Ser Ser Ser His Leu Val Ser Arg Pro Ser Thr Gly Gly 145 150 155 160 Ser Gly Ser Ser Gly Gly Ser Ala Ser Lys Met Ala Glu Gln Gln Lys 165 170 175 Ile Lys Lys Ser Pro His Val Leu Leu Ile Pro Phe Pro Leu Gln Gly 180 185 190

1620

1680

1740

1800

1860

1893

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His Ile Asn 195 Pro Phe Ile Gl n Phe 200 Gly Lys Arg Leu Ile 205 Ser Lys Gly Val Lys Thr Thr Leu Val Thr Thr Ile His Thr Leu Asn Ser Thr Leu 210 215 220 Asn His Ser Asn Thr Thr Thr Thr Ser Ile Glu Ile Gln Ala Ile Ser 225 230 235 240 Asp Gly cys Asp Glu Gly Gly Phe Met Ser Ala Gly Glu Ser Tyr Leu 245 250 255 Glu Thr Phe Lys Gln Val Gly Ser Lys Ser Leu Ala Asp Leu Ile Lys 260 265 270 Lys Leu Gln Ser Glu Gly Thr Thr Ile Asp Ala Ile Ile Tyr Asp Ser 275 280 285 Met Thr Glu T rp Val Leu Asp Val Ala Ile Glu Phe Gly Ile Asp Gly 290 295 300 Gly Ser Phe Phe Thr Gln Ala cys Val Val Asn Ser Leu Tyr Tyr His 305 310 315 320 Val His Lys Gly Leu Ile Ser Leu Pro Leu Gly Glu Thr Val Ser Val 325 330 335 Pro Gly Phe Pro Val Leu Gl n Arg T rp Glu Thr Pro Leu Ile Leu Gln 340 345 350 Asn His Glu Gln Ile Gln Ser Pro T rp Ser Gln Met Leu Phe Gly Gln 355 360 365 Phe Ala Asn Ile Asp Gln Ala Arg T rp Val Phe Thr Asn Ser Phe Tyr 370 375 380 Lys Leu Glu Glu Glu Val Ile Glu T rp Thr Arg Lys Ile T rp Asn Leu 385 390 395 400 Lys Val Ile Gly Pro Thr Leu Pro Ser Met Tyr Leu Asp Lys Arg Leu 405 410 415 Asp Asp Asp Lys Asp Asn Gly Phe Asn Leu Tyr Lys Ala Asn His His 420 425 430 Glu cys Met Asn T rp Leu Asp Asp Lys Pro Lys Glu Ser Val Val Tyr 435 440 445 Val Ala Phe Gly Ser Leu Val Lys His Gly Pro Glu Gln Val Glu Glu

450 455 460

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Ile 465 Thr Arg Ala Leu Ile Asp Ser Asp Val 470 Asn 475 Phe Leu T rp Val Ile 480 Lys His Lys Glu Glu Gly Lys Leu Pro Glu Asn Leu Ser Glu Val Ile 485 490 495 Lys Thr Gly Lys Gly Leu Ile Val Ala T rp cys Lys Gln Leu Asp Val 500 505 510 Leu Ala His Glu Ser Val Gly cys Phe Val Thr His cys Gly Phe Asn 515 520 525 Ser Thr Leu Glu Ala Ile Ser Leu Gly Val Pro Val Val Ala Met Pro 530 535 540 Gln Phe Ser Asp Gln Thr Thr Asn Ala Lys Leu Leu Asp Glu Ile Leu 545 550 555 560 Gly Val Gly Val Arg Val Lys Ala Asp Glu Asn Gly Ile Val Arg Arg 565 570 575 Gly Asn Leu Ala Ser cys Ile Lys Met Ile Met Glu Glu Glu Arg Gly 580 585 590 Val Ile Ile Arg Lys Asn Ala Val Lys T rp Lys Asp Leu Ala Lys Val 595 600 605 Ala Val His Glu Gly Gly Ser Ser Asp Asn Asp Ile Val Glu Phe Val 610 615 620 Ser Glu Leu Ile Lys Ala

625 630 <210> 91 <211> 1932 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 91 atgtgcaata ccaacatgtc tgtacctact gatggtgctg taaccacctc acagattcca 60 gcttcggaac aagagaccct ggttagacca aagccattgc ttttgaagtt attaaagtct 120 gttggtgcac aaaaagacac ttatactatg aaagaggttc ttttttatct tggccagtat 180 attatgacta aacgattata tgatgagaag caacaacata ttgtatattg ttcaaatgat 240 cttctaggag atttgtttgg cgtgccaagc ttctctgtga aagagcacag gaaaatatat 300 accatgatct acaggaactt ggtagtagtc aatcagcagg aatcatcgga ctcaggtaca 360 tctgtgagtg agaacaggtg tcaccttgaa ggtgggagtg atcaaaagga ccttgtacaa 420

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gagcttcagg aagagaaacc ttcatcttca catttggttt ctagaccatc taccggtggt 480 agcggatcct ctggaggcag tgctagtaaa atggctacat ctgattctat tgttgatgac 540 aggaagcagt tgcatgtggc tactttccct tggcttgctt tcggtcatat actgccttac 600 ctacaactat caaaactgat agctgaaaaa ggacataaag tgtcattcct ttcaacaact 660 agaaacattc aaagattatc ttcccacata tcaccattga ttaacgtcgt tcaattgaca 720 cttccaagag tacaggaatt accagaagat gctgaagcta caacagatgt gcatcctgaa 780 gatatccctt acttgaaaaa ggcatccgat ggattacagc ctgaggtcac tagattcctt 840 gagcaacaca gtccagattg gatcatatac gactacactc actattggtt gccttcaatt 900 gcagcatcac taggcatttc tagggcacat ttcagtgtaa ccacaccttg ggccattgct 960 tacatgggtc catccgctga tgctatgatt aacggcagtg atggtagaac taccgttgaa 1020 gatttgacaa ccccaccaaa gtggtttcca tttccaacta aagtctgttg gagaaaacac 1080 gacttagcaa gactggttcc atacaaggca ccaggaatct cagacggcta tagaatgggt 1140 ttagtcctta aagggtctga ctgcctattg tctaagtgtt accatgagtt tgggacacaa 1200 tggctaccac ttttggaaac attacaccaa gttcctgtcg taccagttgg tctattacct 1260 ccagaaatcc ctggtgatga gaaggacgag acttgggttt caatcaaaaa gtggttagac 1320 gggaagcaaa aaggctcagt ggtatatgtg gcactgggtt ccgaagtttt agtatctcaa 1380 acagaagttg tggaacttgc cttaggtttg gaactatctg gattgccatt tgtctgggcc 1440 tacagaaaac caaaaggccc tgcaaagtcc gattcagttg aattgccaga cggctttgtc 1500 gagagaacta gagatagagg gttggtatgg acttcatggg ctccacaatt gagaatcctg 1560 agtcacgaat ctgtgtgcgg tttcctaaca cattgtggtt ctggttctat agttgaagga 1620 ctgatgtttg gtcatccact tatcatgttg ccaatctttg gtgaccagcc tttgaatgca 1680 cgtctgttag aagataaaca agttggaatt gaaatcccac gtaatgagga agatggatgt 1740 ttaaccaagg agtctgtggc cagatcatta cgttccgttg tcgttgaaaa ggaaggcgaa 1800 atctacaagg ccaatgcccg tgaactttca aagatctaca atgacacaaa agtagagaag 1860 gaatatgttt ctcaatttgt agattaccta gagaaaaacg ctagagccgt agctattgat 1920 catgaatcct aa 1932

<210> 92 <211> 643 <212> PRT <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polypepti de

<400> 92 Met Cys Asn Thr Asn Met Ser Val Pro Thr Asp Gly Ala Val Thr Thr 1 5 10 15 Ser Gln Ile Pro Ala Ser Gl u Gln Glu Thr Leu Val Arg Pro Lys Pro 20 25 30

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Leu Leu

Thr Met 50

Arg Leu 65

Leu Leu

Arg Lys

Gln Glu

Leu Glu 130

Glu Lys 145

Leu Lys 35

Lys Glu

Tyr Asp

Gly Asp

Ile Tyr 100

Ser Ser 115

Gly Gly

Pro Ser

Leu Leu

Val Leu

Glu Lys 70

Leu Phe 85

Thr Met

Asp Ser

Ser Asp

Ser Ser 150

Ser Gly

Ser Ser

Gly Gly 165

Lys Ser 40

Phe Tyr 55

Gln Gln

Gly Val

Ile Tyr

Gly Thr 120

Gl n Lys 135

His Leu

Ser Ala

Val Gly

Ala Gln

Ile Val

Asp Asp 180

Arg Lys

Gln Leu

Ala Phe

Gly His 195

Ile Leu

Pro Tyr 200

Leu Gly

Gln Tyr 60

Glu Lys 210

Gly His

Lys Val

Ser Phe 215

Arg Leu 225

Leu Pro

Val His

Gln Pro

Ile Tyr 290

Ser Ser

His Ile 230

Ser Pro

Arg Val

Pro Glu 260

Glu Val 275

Asp Tyr

Gln Glu 245

Leu Pro

Asp Ile

Thr Arg

Thr His

Pro Tyr

Phe Leu 280

Tyr Trp 295

His Ile

Pro Ser 90

Arg Asn 105

Ser Val

Asp Leu

Val Ser

Val Tyr 75

Phe Ser

Leu Val

Ser Lys 170

His Val 185

Leu Gln

Leu Ser

Leu Ile

Glu Asp 250

Leu Lys 265

Glu Gln

Leu Pro

Lys Asp 45

Ile Met cys Ser

Val Lys

Val Val 110

Thr Tyr

Thr Lys

Asn Asp 80

Glu His 95

Asn Gln

Ser Glu

Val Gln 140

Arg Pro 155

Met Ala

Ala Thr

Leu Ser

Thr Thr 220

Asn Val 235

Ala Glu

Lys Ala

Asn Arg 125

Glu Leu

Ser Thr

Thr Ser

His Ser

Ser Ile 300

Phe Pro 190

Lys Leu 205

Arg Asn

Val Gln

Ala Thr

Ser Asp 270

Pro Asp 285

Ala Ala cys His

Gln Glu

Gly Gly 160

Asp Ser 175

Trp Leu

Ile Ala

Ile Gln

Leu Thr 240

Thr Asp 255

Gly Leu

Trp Ile

Ser Leu

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Gly 305 Ile Ser Arg Ala His 310 Phe Ser Val Thr Thr 315 Pro T rp Ala Ile Ala 320 Tyr Met Gly Pro Ser Ala Asp Ala Met Ile Asn Gly Ser Asp Gly Arg 325 330 335 Thr Thr Val Glu Asp Leu Thr Thr Pro Pro Lys T rp Phe Pro Phe Pro 340 345 350 Thr Lys Val cys T rp Arg Lys His Asp Leu Ala Arg Leu Val Pro Tyr 355 360 365 Lys Ala Pro Gly Ile Ser Asp Gly Tyr Arg Met Gly Leu Val Leu Lys 370 375 380 Gly Ser Asp cys Leu Leu Ser Lys cys Tyr His Glu Phe Gly Thr Gln 385 390 395 400 T rp Leu Pro Leu Leu Glu Thr Leu His Gln Val Pro Val Val Pro Val 405 410 415 Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp Glu Lys Asp Glu Thr T rp 420 425 430 Val Ser Ile Lys Lys T rp Leu Asp Gly Lys Gln Lys Gly Ser Val Val 435 440 445 Tyr Val Ala Leu Gly Ser Gl u Val Leu Val Ser Gln Thr Glu Val Val 450 455 460 Glu Leu Ala Leu Gly Leu Gl u Leu Ser Gly Leu Pro Phe Val T rp Ala 465 470 475 480 Tyr Arg Lys Pro Lys Gly Pro Ala Lys Ser Asp Ser Val Glu Leu Pro 485 490 495 Asp Gly Phe Val Glu Arg Thr Arg Asp Arg Gly Leu Val T rp Thr Ser 500 505 510 T rp Ala Pro Gln Leu Arg Ile Leu Ser His Glu Ser Val cys Gly Phe 515 520 525 Leu Thr His cys Gly Ser Gly Ser Ile Val Glu Gly Leu Met Phe Gly 530 535 540 His Pro Leu Ile Met Leu Pro Ile Phe Gly Asp Gln Pro Leu Asn Ala 545 550 555 560 Arg Leu Leu Glu Asp Lys Gl n Val Gly Ile Glu Ile Pro Arg Asn Glu 565 570 575

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Glu Asp Gly cys Leu Thr Lys Glu Ser Val Ala Arg Ser Leu Arg Ser 580 585 590 Val Val Val Glu Lys Glu Gly Glu Ile Tyr Lys Ala Asn Ala Arg Glu 595 600 605 Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val Glu Lys Glu Tyr Val Ser 610 615 620 Gln Phe Val Asp Tyr Leu Gl u Lys Asn Ala Arg Ala Val Ala Ile Asp 625 630 635 640

His Glu Ser <210> 93 <211> 1887 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 93 atgtgcaata ccaacatgtc tgtacctact gatggtgctg taaccacctc acagattcca 60 gcttcggaac aagagaccct ggttagacca aagccattgc ttttgaagtt attaaagtct 120 gttggtgcac aaaaagacac ttatactatg aaagaggttc ttttttatct tggccagtat 180 attatgacta aacgattata tgatgagaag caacaacata ttgtatattg ttcaaatgat 240 cttctaggag atttgtttgg cgtgccaagc ttctctgtga aagagcacag gaaaatatat 300 accatgatct acaggaactt ggtagtagtc aatcagcagg aatcatcgga ctcaggtaca 360 tctgtgagtg agaacaggtg tcaccttgaa ggtgggagtg atcaaaagga ccttgtacaa 420 gagcttcagg aagagaaacc ttcatcttca catttggttt ctagaccatc taccggtggt 480 agcggatcct ctggaggcag tgctagtaaa atggaaaaca agaccgaaac aacagttaga 540 cgtaggcgta gaatcattct gtttccagta ccttttcaag ggcacatcaa tccaatacta 600 caactagcca acgttttgta ctctaaaggt ttttctatta caatctttca caccaatttc 660 aacaaaccaa aaacatccaa ttacccacat ttcacattca gattcatact tgataatgat 720 ccacaagatg aacgtatttc aaacttacct acccacggtc ctttagctgg aatgagaatt 780 ccaatcatca atgaacatgg tgccgatgag cttagaagag aattagagtt acttatgttg 840 gcatccgaag aggacgagga agtctcttgt ctgattactg acgctctatg gtactttgcc 900 caatctgtgg ctgatagttt gaatttgagg agattggtac taatgacatc cagtctgttt 960 aactttcacg ctcatgttag tttaccacaa tttgacgaat tgggatactt ggaccctgat 1020 gacaagacta ggttagagga acaggcctct ggttttccta tgttgaaagt caaagatatc 1080 aagtctgcct attctaattg gcaaatcttg aaagagatct taggaaagat gatcaaacag 1140 acaaaggctt catctggagt gatttggaac agtttcaaag agttagaaga gtctgaattg 1200

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gagactgtaa tcagagaaat tccagcacct tcattcctga taccattacc aaaacatttg 1260 actgcttcct cttcctcttt gttggatcat gacagaacag tttttcaatg gttggaccaa 1320 caaccaccta gttctgtttt gtacgtgtca tttggtagta cttctgaagt cgatgaaaag 1380 gacttccttg aaatcgcaag aggcttagtc gatagtaagc agtcattcct ttgggtcgtg 1440 cgtccaggtt tcgtgaaagg ctcaacatgg gtcgaaccac ttccagatgg ttttctaggc 1500 gaaagaggta gaatagtcaa atgggttcct caacaggaag ttttagctca tggcgctatt 1560 ggggcattct ggactcattc cggatggaat tcaactttag aatcagtatg cgaaggggta 1620 cctatgatct tttcagattt tggtcttgat caaccactga acgcaagata catgtctgat 1680 gttttgaaag tgggtgtata tctagaaaat ggctgggaaa ggggtgaaat agctaatgca 1740 ataagacgtg ttatggttga tgaagagggg gagtatatca gacaaaacgc aagagtgctg 1800 aagcaaaagg ccgacgtttc tctaatgaag ggaggctctt catacgaatc cttagaatct 1860 cttgtttcct acatttcatc actgtaa 1887

<210> 94 <211> 628 <212> PRT <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polypepti de

<400> 94 Thr Asn Met Ser 5 Val Pro Thr 10 Asp Gly Ala Val Thr 15 Thr Met 1 cys Asn Ser Gln Ile Pro Ala Ser Gl u Gln Glu Thr Leu Val Arg Pro Lys Pro 20 25 30 Leu Leu Leu Lys Leu Leu Lys Ser Val Gly Ala Gln Lys Asp Thr Tyr 35 40 45 Thr Met Lys Glu Val Leu Phe Tyr Leu Gly Gln Tyr Ile Met Thr Lys 50 55 60 Arg Leu Tyr Asp Glu Lys Gl n Gln His Ile Val Tyr cys Ser Asn Asp 65 70 75 80 Leu Leu Gly Asp Leu Phe Gly Val Pro Ser Phe Ser Val Lys Glu His 85 90 95 Arg Lys Ile Tyr Thr Met Ile Tyr Arg Asn Leu Val Val Val Asn Gln 100 105 110 Gln Glu Ser Ser Asp Ser Gly Thr Ser Val Ser Glu Asn Arg cys His 115 120 125 Leu Glu Gly Gly Ser Asp Gl n Lys Asp Leu Val Gln Glu Leu Gln Glu Page 102

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130 135 140

Glu 145 Lys Pro Ser Ser Ser 150 His Leu Val Ser Arg 155 Pro Ser Thr Gly Gly 160 Ser Gly Ser Ser Gly Gly Ser Ala Ser Lys Met Glu Asn Lys Thr Glu 165 170 175 Thr Thr Val Arg Arg Arg Arg Arg Ile Ile Leu Phe Pro Val Pro Phe 180 185 190 Gln Gly His Ile Asn Pro Ile Leu Gln Leu Ala Asn Val Leu Tyr Ser 195 200 205 Lys Gly Phe Ser Ile Thr Ile Phe His Thr Asn Phe Asn Lys Pro Lys 210 215 220 Thr Ser Asn Tyr Pro His Phe Thr Phe Arg Phe Ile Leu Asp Asn Asp 225 230 235 240 Pro Gln Asp Glu Arg Ile Ser Asn Leu Pro Thr His Gly Pro Leu Ala 245 250 255 Gly Met Arg Ile Pro Ile Ile Asn Glu His Gly Ala Asp Glu Leu Arg 260 265 270 Arg Glu Leu Glu Leu Leu Met Leu Ala Ser Glu Glu Asp Glu Glu Val 275 280 285 Ser cys Leu Ile Thr Asp Ala Leu T rp Tyr Phe Ala Gln Ser Val Ala 290 295 300 Asp Ser Leu Asn Leu Arg Arg Leu Val Leu Met Thr Ser Ser Leu Phe 305 310 315 320 Asn Phe His Ala His Val Ser Leu Pro Gln Phe Asp Glu Leu Gly Tyr 325 330 335 Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu Glu Gln Ala Ser Gly Phe 340 345 350 Pro Met Leu Lys Val Lys Asp Ile Lys Ser Ala Tyr Ser Asn T rp Gln 355 360 365 Ile Leu Lys Glu Ile Leu Gly Lys Met Ile Lys Gln Thr Lys Ala Ser 370 375 380 Ser Gly Val Ile T rp Asn Ser Phe Lys Glu Leu Glu Glu Ser Glu Leu 385 390 395 400 Glu Thr Val Ile Arg Glu Ile Pro Ala Pro Ser Phe Leu Ile Pro Leu 405 410 415

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Pro Lys His Leu Thr Ala Ser Ser Ser Ser Leu Leu Asp His Asp Arg 420 425 430 Thr Val Phe Gln T rp Leu Asp Gln Gln Pro Pro Ser Ser Val Leu Tyr 435 440 445 Val Ser Phe Gly Ser Thr Ser Glu Val Asp Glu Lys Asp Phe Leu Glu 450 455 460 Ile Ala Arg Gly Leu Val Asp Ser Lys Gln Ser Phe Leu T rp Val Val 465 470 475 480 Arg Pro Gly Phe Val Lys Gly Ser Thr T rp Val Glu Pro Leu Pro Asp 485 490 495 Gly Phe Leu Gly Glu Arg Gly Arg Ile Val Lys T rp Val Pro Gln Gln 500 505 510 Glu Val Leu Ala His Gly Ala Ile Gly Ala Phe T rp Thr His Ser Gly 515 520 525 T rp Asn Ser Thr Leu Glu Ser Val cys Glu Gly Val Pro Met Ile Phe 530 535 540 Ser Asp Phe Gly Leu Asp Gl n Pro Leu Asn Ala Arg Tyr Met Ser Asp 545 550 555 560 Val Leu Lys Val Gly Val Tyr Leu Glu Asn Gly T rp Glu Arg Gly Glu 565 570 575 Ile Ala Asn Ala Ile Arg Arg Val Met Val Asp Glu Glu Gly Glu Tyr 580 585 590 Ile Arg Gln Asn Ala Arg Val Leu Lys Gln Lys Ala Asp Val Ser Leu 595 600 605 Met Lys Gly Gly Ser Ser Tyr Glu Ser Leu Glu Ser Leu Val Ser Tyr 610 615 620

Ile Ser Ser Leu 625 <210> 95 <211> 473 <212> PRT <213> Stevia rebaudiana <400> 95

Met 1 Ala Thr Ser Asp 5 Ser Ile Val Asp Asp Arg Lys 10 Gln Leu His 15 Val Ala Thr Phe Pro T rp Leu Ala Phe Gly His Ile Leu Page 104 Pro Tyr Leu Gln

2018200459 19 Jan 2018

20 25 30 Leu Ser Lys Leu Ile Ala Gl u Lys Gly His Lys Val Ser Phe Leu Ser 35 40 45 Thr Thr Arg Asn Ile Gln Arg Leu Ser Ser His Ile Ser Pro Leu Ile 50 55 60 Asn Val Val Gln Leu Thr Leu Pro Arg Val Gln Glu Leu Pro Glu Asp 65 70 75 80 Ala Glu Ala Thr Thr Asp Val His Pro Glu Asp Ile Pro Tyr Leu Lys 85 90 95 Lys Ala Ser Asp Gly Leu Gl n Pro Glu Val Thr Arg Phe Leu Glu Gln 100 105 110 His Ser Pro Asp T rp Ile Ile Tyr Asp Tyr Thr His Tyr T rp Leu Pro 115 120 125 Ser Ile Ala Ala Ser Leu Gly Ile Ser Arg Ala His Phe Ser Val Thr 130 135 140 Thr Pro T rp Ala Ile Ala Tyr Met Gly Pro Ser Ala Asp Ala Met Ile 145 150 155 160 Asn Gly Ser Asp Gly Arg Thr Thr Val Glu Asp Leu Thr Thr Pro Pro 165 170 175 Lys T rp Phe Pro Phe Pro Thr Lys Val cys T rp Arg Lys His Asp Leu 180 185 190 Ala Arg Leu Val Pro Tyr Lys Ala Pro Gly Ile Ser Asp Arg cys Arg 195 200 205 Met Gly Leu Val Leu Lys Gly Ser Asp cys Leu Leu Ser Lys cys Tyr 210 215 220 His Glu Phe Gly Thr Gln Trp Leu Pro Leu Leu Glu Thr Leu His Gln 225 230 235 240 Val Pro Val Val Pro Val Gly Leu Leu Pro Pro Glu Ile Pro Gly Asp 245 250 255 Glu Lys Asp Glu Thr T rp Val Ser Ile Lys Lys T rp Leu Asp Gly Lys 260 265 270 Gln Lys Gly Ser Val Val Tyr Val Ala Leu Gly Ser Glu Val Leu Val 275 280 285 Ser Gln Thr Glu Val Val Gl u Leu Ala Leu Gly Leu Glu Leu Ser Gly

290 295 300

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Leu 305 Pro Phe Val Trp Ala 310 Tyr Arg Lys Pro Lys 315 Gly Pro Ala Lys Ser 320 Asp Ser Val Glu Leu Pro Asp Gly Phe Val Glu Arg Thr Arg Asp Arg 325 330 335 Gly Leu Val T rp Thr Ser Arg Ala Pro Gln Leu Arg Ile Leu Ser His 340 345 350 Glu Ser Val cys Gly Phe Leu Thr His cys Gly Ser Gly Ser Ile Val 355 360 365 Glu Gly Leu Met Phe Gly His Pro Leu Ile Met Leu Pro Ile Phe Gly 370 375 380 Asp Gln Pro Leu Asn Ala Arg Leu Leu Glu Asp Lys Gln Val Gly Ile 385 390 395 400 Glu Ile Pro Arg Asn Glu Gl u Asp Gly cys Leu Thr Lys Glu Ser Val 405 410 415 Ala Arg Ser Leu Arg Ser Val Val Val Glu Lys Glu Gly Glu Ile Tyr 420 425 430 Lys Ala Asn Ala Arg Glu Leu Ser Lys Ile Tyr Asn Asp Thr Lys Val 435 440 445 Glu Lys Glu Tyr Val Ser Gl n Phe Val Asp Tyr Leu Glu Lys Asn Ala 450 455 460 Arg Ala Val Ala Ile Asp His Glu Ser 465 470

<210> 96 <211> 1422 <212> DNA <213> Stevia rebaudiana <400> 96

atggctacca gtgactccat agttgacgac cgtaagcagc ttcatgttgc gacgttccca 60 tggcttgctt tcggtcacat cctcccttac cttcagcttt cgaaattgat agctgaaaag 120 ggtcacaaag tctcgtttct ttctaccacc agaaacattc aacgtctctc ttctcatatc 180 tcgccactca taaatgttgt tcaactcaca cttccacgtg tccaagagct gccggaggat 240 gcagaggcga ccactgacgt ccaccctgaa gatattccat atctcaagaa ggcttctgat 300 ggtcttcaac cggaggtcac ccggtttcta gaacaacact ctccggactg gattatttat 360 gattatactc actactggtt gccatccatc gcggctagcc tcggtatctc acgagcccac 420 ttctccgtca ccactccatg ggccattgct tatatgggac cctcagctga cgccatgata 480 aatggttcag atggtcgaac cacggttgag gatctcacga caccgcccaa gtggtttccc 540

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2018200459 19 Jan 2018 tttccgacca aagtatgctg gcggaagcat gatcttgccc gactggtgcc ttacaaagct 600 ccggggatat ctgatcgatg ccgtatgggg ctggttctta agggatctga ttgtttgctc 660 tccaaatgtt accatgagtt tggaactcaa tggctacctc ttttggagac actacaccaa 720 gtaccggtgg ttccggtggg attactgcca ccggaaatac ccggagacga gaaagatgaa 780 acatgggtgt caatcaagaa atggctcgat ggtaaacaaa aaggcagtgt ggtgtacgtt 840 gcattaggaa gcgaggtttt ggtgagccaa accgaggttg ttgagttagc attgggtctc 900 gagctttctg ggttgccatt tgtttgggct tatagaaaac caaaaggtcc cgcgaagtca 960 gactcggtgg agttgccaga cgggttcgtg gaacgaactc gtgaccgtgg gttggtctgg 1020 acgagtcggg cacctcagtt acgaatactg agccatgagt cggtttgtgg gttcttgacg 1080 cattgtggtt ctggatcaat tgtggaaggg ctaatgtttg gtcaccctct aatcatgcta 1140 ccgatttttg gggaccaacc tctgaatgct cgattactgg aggacaaaca ggtgggaatc 1200 gagataccaa gaaatgagga agatggttgc ttgaccaagg agtcggttgc tagatcactg 1260 aggtccgttg ttgtggaaaa agaaggggag atctacaagg cgaacgcgag ggagctgagt 1320 aaaatctata acgacactaa ggttgaaaaa gaatatgtaa gccaattcgt agactatttg 1380 gaaaagaatg cgcgtgcggt tgccatcgat catgagagtt aa 1422 <210> 97 <211> 1380 <212> DNA <213> Stevia rebaudiana <400> 97 atggaaaata aaacggagac caccgttcgc cggcgccgga gaataatatt attcccggta 60 ccatttcaag gccacattaa cccaattctt cagctagcca atgtgttgta ctctaaagga 120 ttcagtatca ccatctttca caccaacttc aacaaaccca aaacatctaa ttaccctcac 180 ttcactttca gattcatcct cgacaacgac ccacaagacg aacgcatttc caatctaccg 240 actcatggtc cgctcgctgg tatgcggatt ccgattatca acgaacacgg agctgacgaa 300 ttacgacgcg aactggaact gttgatgtta gcttctgaag aagatgaaga ggtatcgtgt 360 ttaatcacgg atgctctttg gtacttcgcg caatctgttg ctgacagtct taacctccga 420 cggcttgttt tgatgacaag cagcttgttt aattttcatg cacatgtttc acttcctcag 480 tttgatgagc ttggttacct cgatcctgat gacaaaaccc gtttggaaga acaagcgagt 540 gggtttccta tgctaaaagt gaaagacatc aagtctgcgt attcgaactg gcaaatactc 600 aaagagatat tagggaagat gataaaacaa acaaaagcat cttcaggagt catctggaac 660 tcatttaagg aactcgaaga gtctgagctc gaaactgtta tccgtgagat cccggctcca 720 agtttcttga taccactccc caagcatttg acagcctctt ccagcagctt actagaccac 780 gatcgaaccg tttttccatg gttagaccaa caaccgtcac gttcggtact gtatgttagt 840 tttggtagtg gtactgaagt actggatgag aaagatttct tggaaatagc tcgtgggttg 900 gttgatagca agcagtcgtt tttatgggtg gttcgacctg ggtttgtcaa gggttcgacg 960

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tgggtcgaac cgttgccaga tgggttcttg ggtgaaagag gacgtattgt gaaatgggtt 1020 ccacagcaag aagtgctagc tcatggagca ataggcgcat tctggactca tagcggatgg 1080 aactctacgt tggaaagcgt ttgtgaaggt gttcctatga ttttctcgga ttttgggctc 1140 gatcaaccgt tgaatgctag atacatgagt gatgttttga aggtaggggt gtatttggaa 1200 aatgggtggg aaagaggaga gatagcaaat gcaataagaa gagttatggt ggatgaagaa 1260 ggagaataca ttagacagaa tgcaagagtt ttgaaacaaa aggcagatgt ttctttgatg 1320 aagggtggtt cgtcttacga atcattagag tctctagttt cttacatttc atcgttgtaa 1380

<210> 98 <211> 459 <212> PRT <213> Stevia rebaudiana

<400> 98 Arg Arg Arg 10 Arg Arg Ile 15 Ile Met 1 Glu Asn Lys Thr 5 Glu Thr Thr Val Leu Phe Pro Val Pro Phe Gl n Gly His Ile Asn Pro Ile Leu Gln Leu 20 25 30 Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr 35 40 45 Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg 50 55 60 Phe Ile Leu Asp Asn Asp Pro Gln Asp Glu Arg Ile Ser Asn Leu Pro 65 70 75 80 Thr His Gly Pro Leu Ala Gly Met Arg Ile Pro Ile Ile Asn Glu His 85 90 95 Gly Ala Asp Glu Leu Arg Arg Glu Leu Glu Leu Leu Met Leu Ala Ser 100 105 110 Glu Glu Asp Glu Glu Val Ser cys Leu Ile Thr Asp Ala Leu T rp Tyr 115 120 125 Phe Ala Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu 130 135 140 Met Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln 145 150 155 160 Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu 165 170 175 Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Ser

180 185 190

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Ala Tyr Ser Asn Trp Gln Ile 195 Leu 200 Lys Glu Ile Leu Gly 205 Lys Met Ile Lys Gln Thr Lys Ala Ser Ser Gly Val Ile T rp Asn Ser Phe Lys Glu 210 215 220 Leu Glu Glu Ser Glu Leu Gl u Thr Val Ile Arg Glu Ile Pro Ala Pro 225 230 235 240 Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser 245 250 255 Leu Leu Asp His Asp Arg Thr Val Phe Pro T rp Leu Asp Gln Gln Pro 260 265 270 Ser Arg Ser Val Leu Tyr Val Ser Phe Gly Ser Gly Thr Glu Val Leu 275 280 285 Asp Glu Lys Asp Phe Leu Gl u Ile Ala Arg Gly Leu Val Asp Ser Lys 290 295 300 Gln Ser Phe Leu T rp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr 305 310 315 320 T rp Val Glu Pro Leu Pro Asp Gly Phe Leu Gly Glu Arg Gly Arg Ile 325 330 335 Val Lys T rp Val Pro Gln Gl n Glu Val Leu Ala His Gly Ala Ile Gly 340 345 350 Ala Phe T rp Thr His Ser Gly T rp Asn Ser Thr Leu Glu Ser Val cys 355 360 365 Glu Gly Val Pro Met Ile Phe Ser Asp Phe Gly Leu Asp Gln Pro Leu 370 375 380 Asn Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu 385 390 395 400 Asn Gly T rp Glu Arg Gly Gl u Ile Ala Asn Ala Ile Arg Arg Val Met 405 410 415 Val Asp Glu Glu Gly Glu Tyr Ile Arg Gln Asn Ala Arg Val Leu Lys 420 425 430 Gln Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser 435 440 445 Leu Glu Ser Leu Val Ser Tyr Ile Ser Ser Leu 450 455

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2018200459 19 Jan 2018 <210> 99 <211> 1380 <212> DNA <213> Stevia rebaudiana <220>

<221> modified_base <222> (861)..(863) <223> a, c, t, g, unknown or other <400> 99

atggaaaata aaacggagac caccgttcgc cggcgccgga gaataatatt attcccggta 60 ccatttcaag gccacattaa cccaattctt cagctagcca atgtgttgta ctctaaagga 120 ttcagtatca ccatctttca caccaacttc aacaaaccca aaacatctaa ttaccctcac 180 ttcactttca gattcatcct cgacaacgac ccacaagacg aacgcatttc caatctaccg 240 actcatggtc cgctcgctgg tatgcggatt ccgattatca acgaacacgg agctgacgaa 300 ttacgacgcg aactggaact gttgatgtta gcttctgaag aagatgaaga ggtatcgtgt 360 ttaatcacgg atgctctttg gtacttcgcg caatctgttg ctgacagtct taacctccga 420 cggcttgttt tgatgacaag cagcttgttt aattttcatg cacatgtttc acttcctcag 480 tttgatgagc ttggttacct cgatcctgat gacaaaaccc gtttggaaga acaagcgagt 540 gggtttccta tgctaaaagt gaaagacatc aagtctgcgt attcgaactg gcaaatactc 600 aaagagatat tagggaagat gataaaacaa acaaaagcat cttcaggagt catctggaac 660 tcatttaagg aactcgaaga gtctgagctc gaaactgtta tccgtgagat cccggctcca 720 agtttcttga taccactccc caagcatttg acagcctctt ccagcagctt actagaccac 780 gatcgaaccg tttttcaatg gttagaccaa caaccgccaa gttcggtact gtatgttagt 840 tttggtagta ctagtgaagt nnnggatgag aaagatttct tggaaatagc tcgtgggttg 900 gttgatagca agcagtcgtt tttatgggtg gttcgacctg ggtttgtcaa gggttcgacg 960 tgggtcgaac cgttgccaga tgggttcgtg gccgaaagag ggcgtattgt gaaatgggtt 1020 ccgcaacagg aagtgatagc tcatggagca atcggtgcat tctggactca tagcggatgg 1080 aactctacat tggaaagcgt ttgtgaaggt gttcctatga ttttctcgga ttttgggctc 1140 gatcaaccgt tgaatgctag atacatgagt gatgttttga aggtaggggt gtatttggaa 1200 aatgggtggg aaagaggaga gatagcaaat gcaatacgaa gagttatggt ggatgaagaa 1260 ggagaataca ttagacagaa tgcaagagtt ttgaaacaaa aggcagatgt ttctttgatg 1320 aagggtggtt catcttacga atcattagag tctctagttt cttacatttc atcgttgtaa 1380

<210> 100 <211> 459 <212> PRT <213> Stevia rebaudiana <220>

<221> MOD_RES <222> (288)..(288) <223> Any amino acid

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<400> 100 Thr 5 Glu Thr Thr Val Arg Arg Arg Arg Arg 10 Ile 15 Ile Met 1 Glu Asn Lys Leu Phe Pro Val Pro Phe Gl n Gly His Ile Asn Pro Ile Leu Gln Leu 20 25 30 Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr 35 40 45 Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg 50 55 60 Phe Ile Leu Asp Asn Asp Pro Gln Asp Glu Arg Ile Ser Asn Leu Pro 65 70 75 80 Thr His Gly Pro Leu Ala Gly Met Arg Ile Pro Ile Ile Asn Glu His 85 90 95 Gly Ala Asp Glu Leu Arg Arg Glu Leu Glu Leu Leu Met Leu Ala Ser 100 105 110 Glu Glu Asp Glu Glu Val Ser cys Leu Ile Thr Asp Ala Leu T rp Tyr 115 120 125 Phe Ala Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu 130 135 140 Met Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln 145 150 155 160 Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu 165 170 175 Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Ser 180 185 190 Ala Tyr Ser Asn T rp Gln Ile Leu Lys Glu Ile Leu Gly Lys Met Ile 195 200 205 Lys Gln Thr Lys Ala Ser Ser Gly Val Ile T rp Asn Ser Phe Lys Glu 210 215 220 Leu Glu Glu Ser Glu Leu Gl u Thr Val Ile Arg Glu Ile Pro Ala Pro 225 230 235 240 Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser 245 250 255 Leu Leu Asp His Asp Arg Thr Val Phe Gln T rp Leu Asp Gln Gln Pro 260 265 270

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Pro Ser Ser Val Leu Tyr Val Ser 280 Phe Gly Ser Thr Ser 285 Glu Val Xaa 275 Asp Glu Lys Asp Phe Leu Gl u Ile Ala Arg Gly Leu Val Asp Ser Lys 290 295 300 Gln Ser Phe Leu T rp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr 305 310 315 320 T rp Val Glu Pro Leu Pro Asp Gly Phe Val Ala Glu Arg Gly Arg Ile 325 330 335 Val Lys T rp Val Pro Gln Gl n Glu Val Ile Ala His Gly Ala Ile Gly 340 345 350 Ala Phe T rp Thr His Ser Gly T rp Asn Ser Thr Leu Glu Ser Val cys 355 360 365 Glu Gly Val Pro Met Ile Phe Ser Asp Phe Gly Leu Asp Gln Pro Leu 370 375 380 Asn Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu 385 390 395 400 Asn Gly T rp Glu Arg Gly Gl u Ile Ala Asn Ala Ile Arg Arg Val Met 405 410 415 Val Asp Glu Glu Gly Glu Tyr Ile Arg Gln Asn Ala Arg Val Leu Lys 420 425 430 Gln Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser 435 440 445 Leu Glu Ser Leu Val Ser Tyr Ile Ser Ser Leu

450 455 <210> 101 <211> 1380 <212> DNA <213> Stevia rebaudiana <220>

<221> modified_base <222> (861)..(863) <223> a, c, t, g, unknown or other <400> 101

atggaaaata aaacggagac caccgttcgc cggcgccgga gaataatatt attcccggta 60 ccatttcaag gccacattaa cccaattctt cagctagcca atgtgttgta ctctaaagga 120 ttcagtatca ccatctttca caccaacttc aacaaaccca aaacatctaa ttaccctcac 180 ttcactttca gattcatcct cgacaacgac ccacaagacg aacgcatttc caatctaccg 240

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actcatggtc cgctcgctgg tatgcggatt ccgattatca acgaacacgg agctgacgaa 300 ttacgacgcg aactggaact gttgatgtta gcttctgaag aagatgaaga ggtatcgtgt 360 ttaatcacgg atgctctttg gtacttcgcg caatctgttg ctgacagtct taacctccga 420 cggcttgttt tgatgacaag cagcttgttt aattttcatg cacatgtttc acttcctcag 480 tttgatgagc ttggttacct cgatcctgat gacaaaaccc gtttggaaga acaagcgagt 540 gggtttccta tgctaaaagt gaaagacatc aagtctgcgt attcgaactg gcaaatactc 600 aaagagatat tagggaagat gataaaacaa acaaaagcat cttcaggagt catctggaac 660 tcatttaagg aactcgaaga gtctgagctc gaaactgtta tccgtgagat cccggctcca 720 agtttcttga taccactccc caagcatttg acagcctctt ccagcagctt actagaccac 780 gatcgaaccg tttttcaatg gttagaccaa caaccgccaa gttcggtact gtatgttagt 840 tttggtagta ctagtgaagt nnnggatgag aaagatttct tggaaatagc tcgtgggttg 900 gttgatagca agcagtcgtt tttatgggtg gttcgacctg ggtttgtcaa gggttcgacg 960 tgggtcgaac cgttgccaga tgggttcttg ggtgaaagag gacgtattgt gaaatgggtt 1020 ccacagcaag aagtgctagc tcatggagca ataggcgcat tctggactca tagcggatgg 1080 aactctacgt tggaaagcgt ttgtgaaggt gttcctatga ttttctcgga ttttgggctc 1140 gatcaaccgt tgaatgctag atacatgagt gatgttttga aggtaggggt gtatttggaa 1200 aatgggtggg aaagaggaga gatagcaaat gcaataagaa gagttatggt ggatgaagaa 1260 ggagaataca ttagacagaa tgcaagagtt ttgaaacaaa aggcagatgt ttctttgatg 1320 aagggtggtt cgtcttacga atcattagag tctctagttt cttacatttc atcgttgtaa 1380

<210> 102 <211> 459 <212> PRT <213> Stevia rebaudiana <220>

<221> MOD_RES <222> (288)..(288) <223> Any amino acid

<400> 102 Met 1 Glu Asn Lys Thr Glu 5 Thr Thr Val Arg 10 Arg Arg Arg Arg Ile 15 Ile Leu Phe Pro Val Pro Phe Gl n Gly His Ile Asn Pro Ile Leu Gln Leu 20 25 30 Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr 35 40 45 Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg 50 55 60 Phe Ile Leu Asp Asn Asp Pro Gln Asp Glu Arg Ile Ser Asn Leu Pro 65 70 75 80

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Thr His Gly Pro Leu Ala 85 Gly Met Arg Ile 90 Pro Ile Ile Asn Glu 95 His Gly Ala Asp Glu Leu Arg Arg Glu Leu Glu Leu Leu Met Leu Ala Ser 100 105 110 Glu Glu Asp Glu Glu Val Ser cys Leu Ile Thr Asp Ala Leu T rp Tyr 115 120 125 Phe Ala Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu 130 135 140 Met Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln 145 150 155 160 Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu 165 170 175 Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Ser 180 185 190 Ala Tyr Ser Asn T rp Gln Ile Leu Lys Glu Ile Leu Gly Lys Met Ile 195 200 205 Lys Gln Thr Lys Ala Ser Ser Gly Val Ile T rp Asn Ser Phe Lys Glu 210 215 220 Leu Glu Glu Ser Glu Leu Gl u Thr Val Ile Arg Glu Ile Pro Ala Pro 225 230 235 240 Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser 245 250 255 Leu Leu Asp His Asp Arg Thr Val Phe Gln T rp Leu Asp Gln Gln Pro 260 265 270 Pro Ser Ser Val Leu Tyr Val Ser Phe Gly Ser Thr Ser Glu Val Xaa 275 280 285 Asp Glu Lys Asp Phe Leu Gl u Ile Ala Arg Gly Leu Val Asp Ser Lys 290 295 300 Gln Ser Phe Leu T rp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr 305 310 315 320 T rp Val Glu Pro Leu Pro Asp Gly Phe Leu Gly Glu Arg Gly Arg Ile 325 330 335 Val Lys T rp Val Pro Gln Gl n Glu Val Leu Ala His Gly Ala Ile Gly

340 345 350

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Ala Phe Trp Thr His Ser Gly Trp Asn Ser Thr Leu Glu Ser Val Cys 355 360 365

Glu Gly Val Pro Met Ile Phe Ser Asp Phe Gly Leu Asp Gln Pro Leu 370 375 380

Asn Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu 385 390 395 400

Asn Gly Trp Glu Arg Gly Glu Ile Ala Asn Ala Ile Arg Arg Val Met 405 410 415

Val Asp Glu Glu Gly Glu Tyr Ile Arg Gln Asn Ala Arg Val Leu Lys 420 425 430

Gln Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser 435 440 445

Leu Glu Ser Leu Val Ser Tyr Ile Ser Ser Leu 450 455 <210> 103 <211> 1323 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 103 atgcattcta ccagacatat cttaagacaa agggccgtcc tagttacagg cgctagaaca 60 ccattcgtga aatcatttgg ggctcttatg aaagcagata ccttggaatt ggcatcagca 120 tcagtcgctg ggttgctgaa caagacctca ctggacccta gagatatcga tcatatcgtt 180 tggggtaatg ttgtacttca aggatcagct cataactgcg ccagagaaat agttatcgac 240 cttaacatgc ctaaaaagat catcggtaat ttgacatcta tggcctgtgc ttcaggctta 300 tcttctttgt cacaagcctg tatgctaata gagggtggtc atgccgatgt cgtcattgct 360 ggcggttctg attcagtctc caacactgaa gtgcctttgc caagatccgt cacttacggt 420 ctaatgatgg cccaaaggaa gggtgttatg ggcttcttta aggaagcagg atacaaccca 480 ttcaaatggt ttccaggcgg tattgcttta accgaacgta gtacaggaaa aactatgggt 540 tggcatggag acttaattgc tgagttaaac tctatatcta gagatgacca ggaagccctg 600 gctgtggctt ctcatgcaaa tgctgctaga gcagaaaaag ctgggtactt taaggaggaa 660 attgtacctg tgacaatcga caaaaagggc aaaaagactg aagtaacatg tgatgatgtt 720 atgcaaagag atacagaaaa gatgaaggcc aagatgccat cattgaagcc tgttttcaga 780 aaagagggag gtacaataac agcagccact tccagtactc tgactgatgg tggctctgca 840 atgttggtta tgtcagagga aaaggccaaa aagttgggtt atccaactga tgtctgcgtg 900

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2018200459 19 Jan 2018 aagtcttggt atttcagtgg tatcgatcct tacccacaac ttttgttagc accagttcta ggttggggtc cagctttgaa aaaggccgga ttaaccccta aagatatcga tttgtacgaa attcacgaag catttgctgc acaagttcta gccacaatta agtgtttgaa gtctcaggaa ttcttcgata ggtacgctaa cggtgcaaag ccagtattaa ctgaggatat tgatctttct aaactaaatg ttaatggcgg ttccttagca cttggccacc cattcgccgc tacaggaggt agaatcgtaa tctctctagc aaatgagttg agaagatccg gaaagagaca cgggctggtc agtatttgtg cagctggagg gttaggcgga gtagctatac ttgagcatac agcaagtaag taa <210> 104 <211> 440 <212> PRT <213> Leishmania infantum

<400> 104 Arg Ala Val Leu Val 15 Thr Met 1 His Ser Thr Arg 5 His Ile Leu Arg Gln 10 Gly Ala Arg Thr Pro Phe Val Lys Ser Phe Gly Ala Leu Met Lys Ala 20 25 30 Asp Thr Leu Glu Leu Ala Ser Ala Ser Val Ala Gly Leu Leu Asn Lys 35 40 45 Thr Ser Leu Asp Pro Arg Asp Ile Asp His Ile Val T rp Gly Asn Val 50 55 60 Val Leu Gln Gly Ser Ala His Asn cys Ala Arg Glu Ile Val Ile Asp 65 70 75 80 Leu Asn Met Pro Lys Lys Ile Ile Gly Asn Leu Thr Ser Met Ala cys 85 90 95 Ala Ser Gly Leu Ser Ser Leu Ser Gln Ala cys Met Leu Ile Glu Gly 100 105 110 Gly His Ala Asp Val Val Ile Ala Gly Gly Ser Asp Ser Val Ser Asn 115 120 125 Thr Glu Val Pro Leu Pro Arg Ser Val Thr Tyr Gly Leu Met Met Ala 130 135 140 Gln Arg Lys Gly Val Met Gly Phe Phe Lys Glu Ala Gly Tyr Asn Pro 145 150 155 160 Phe Lys T rp Phe Pro Gly Gly Ile Ala Leu Thr Glu Arg Ser Thr Gly 165 170 175 Lys Thr Met Gly T rp His Gly Asp Leu Ile Ala Glu Leu Asn Ser Ile 180 185 190

960

1020

1080

1140

1200

1260

1320

1323

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Ser Arg Asp Asp 195 Gln Glu Ala Leu Ala Val 200 Ala Ser His 205 Ala Asn Ala Ala Arg Ala Glu Lys Ala Gly Tyr Phe Lys Glu Glu Ile Val Pro Val 210 215 220 Thr Ile Asp Lys Lys Gly Lys Lys Thr Glu Val Thr cys Asp Asp Val 225 230 235 240 Met Gln Arg Asp Thr Glu Lys Met Lys Ala Lys Met Pro Ser Leu Lys 245 250 255 Pro Val Phe Arg Lys Glu Gly Gly Thr Ile Thr Ala Ala Thr Ser Ser 260 265 270 Thr Leu Thr Asp Gly Gly Ser Ala Met Leu Val Met Ser Glu Glu Lys 275 280 285 Ala Lys Lys Leu Gly Tyr Pro Thr Asp Val cys Val Lys Ser T rp Tyr 290 295 300 Phe Ser Gly Ile Asp Pro Tyr Pro Gln Leu Leu Leu Ala Pro Val Leu 305 310 315 320 Gly T rp Gly Pro Ala Leu Lys Lys Ala Gly Leu Thr Pro Lys Asp Ile 325 330 335 Asp Leu Tyr Glu Ile His Gl u Ala Phe Ala Ala Gln Val Leu Ala Thr 340 345 350 Ile Lys cys Leu Lys Ser Gl n Glu Phe Phe Asp Arg Tyr Ala Asn Gly 355 360 365 Ala Lys Pro Val Leu Thr Gl u Asp Ile Asp Leu Ser Lys Leu Asn Val 370 375 380 Asn Gly Gly Ser Leu Ala Leu Gly His Pro Phe Ala Ala Thr Gly Gly 385 390 395 400 Arg Ile Val Ile Ser Leu Ala Asn Glu Leu Arg Arg Ser Gly Lys Arg 405 410 415 His Gly Leu Val Ser Ile cys Ala Ala Gly Gly Leu Gly Gly Val Ala 420 425 430 Ile Leu Glu His Thr Ala Ser Lys 435 440

<210> 105 <211> 1584 <212> DNA

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2018200459 19 Jan 2018 <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 105

atggcagctg accaattggt gaaaactgaa gtcaccaaga agtcttttac tgctcctgta 60 caaaaggctt ctacaccagt tttaaccaat aaaacagtca tttctggatc gaaagtcaaa 120 agtttatcat ctgcgcaatc gagctcatca ggaccttcat catctagtga ggaagatgat 180 tcccgcgata ttgaaagctt ggataagaaa atacgtcctt tagaagaatt agaagcatta 240 ttaagtagtg gaaatacaaa acaattgaag aacaaagagg tcgctgcctt ggttattcac 300 ggtaagttac ctttgtacgc tttggagaaa aaattaggtg atactacgag agcggttgcg 360 gtacgtagga aggctctttc aattttggca gaagctcctg tattagcatc tgatcgttta 420 ccatataaaa attatgacta cgaccgcgta tttggcgctt gttgtgaaaa tgttataggt 480 tacatgcctt tgcccgttgg tgttataggc cccttggtta tcgatggtac atcttatcat 540 ataccaatgg caactacaga gggttgtttg gtagcttctg ccatgcgtgg ctgtaaggca 600 atcaatgctg gcggtggtgc aacaactgtt ttaactaagg atggtatgac aagaggccca 660 gtagtccgtt tcccaacttt gaaaagatct ggtgcctgta agatatggtt agactcagaa 720 gagggacaaa acgcaattaa aaaagctttt aactctacat caagatttgc acgtctgcaa 780 catattcaaa cttgtctagc aggagattta ctcttcatga gatttagaac aactactggt 840 gacgcaatgg gtatgaatat gatttctaaa ggtgtcgaat actcattaaa gcaaatggta 900 gaagagtatg gctgggaaga tatggaggtt gtctccgttt ctggtaacta ctgtaccgac 960 aaaaaaccag ctgccatcaa ctggatcgaa ggtcgtggta agagtgtcgt cgcagaagct 1020 actattcctg gtgatgttgt cagaaaagtg ttaaaaagtg atgtttccgc attggttgag 1080 ttgaacattg ctaagaattt ggttggatct gcaatggctg ggtctgttgg tggatttaac 1140 gcacatgcag ctaatttagt gacagctgtt ttcttggcat taggacaaga tcctgcacaa 1200 aatgttgaaa gttccaactg tataacattg atgaaagaag tggacggtga tttgagaatt 1260 tccgtatcca tgccatccat cgaagtaggt accatcggtg gtggtactgt tctagaacca 1320 caaggtgcca tgttggactt attaggtgta agaggcccgc atgctaccgc tcctggtacc 1380 aacgcacgtc aattagcaag aatagttgcc tgtgccgtct tggcaggtga attatcctta 1440 tgtgctgccc tagcagccgg ccatttggtt caaagtcata tgacccacaa caggaaacct 1500 gctgaaccaa caaaacctaa caatttggac gccactgata taaatcgttt gaaagatggg 1560 tccgtcacct gcattaaatc ctaa 1584

<210> 106 <211> 527 <212> PRT <213> Saccharomyces cerevisiae <400> 106

Met Ala Ala Asp Gln Leu Val Lys Thr Glu Val Thr Lys Lys Ser Phe Page 118

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15 10 15

Thr Ala Pro Val Gln Lys Ala Ser Thr 25 Pro Val Leu Thr Asn 30 Lys Thr 20 Val Ile Ser Gly Ser Lys Val Lys Ser Leu Ser Ser Ala Gln Ser Ser 35 40 45 Ser Ser Gly Pro Ser Ser Ser Ser Glu Glu Asp Asp Ser Arg Asp Ile 50 55 60 Glu Ser Leu Asp Lys Lys Ile Arg Pro Leu Glu Glu Leu Glu Ala Leu 65 70 75 80 Leu Ser Ser Gly Asn Thr Lys Gln Leu Lys Asn Lys Glu Val Ala Ala 85 90 95 Leu Val Ile His Gly Lys Leu Pro Leu Tyr Ala Leu Glu Lys Lys Leu 100 105 110 Gly Asp Thr Thr Arg Ala Val Ala Val Arg Arg Lys Ala Leu Ser Ile 115 120 125 Leu Ala Glu Ala Pro Val Leu Ala Ser Asp Arg Leu Pro Tyr Lys Asn 130 135 140 Tyr Asp Tyr Asp Arg Val Phe Gly Ala cys cys Glu Asn Val Ile Gly 145 150 155 160 Tyr Met Pro Leu Pro Val Gly Val Ile Gly Pro Leu Val Ile Asp Gly 165 170 175 Thr Ser Tyr His Ile Pro Met Ala Thr Thr Glu Gly cys Leu Val Ala 180 185 190 Ser Ala Met Arg Gly cys Lys Ala Ile Asn Ala Gly Gly Gly Ala Thr 195 200 205 Thr Val Leu Thr Lys Asp Gly Met Thr Arg Gly Pro Val Val Arg Phe 210 215 220 Pro Thr Leu Lys Arg Ser Gly Ala cys Lys Ile T rp Leu Asp Ser Glu 225 230 235 240 Glu Gly Gln Asn Ala Ile Lys Lys Ala Phe Asn Ser Thr Ser Arg Phe 245 250 255 Ala Arg Leu Gln His Ile Gl n Thr cys Leu Ala Gly Asp Leu Leu Phe 260 265 270 Met Arg Phe Arg Thr Thr Thr Gly Asp Ala Met Gly Met Asn Met Ile 275 280 285

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Ser Lys 290 Gly Val Glu Tyr Ser 295 Leu Lys Gln Met Val 300 Glu Glu Tyr Gly T rp Glu Asp Met Glu Val Val Ser Val Ser Gly Asn Tyr cys Thr Asp 305 310 315 320 Lys Lys Pro Ala Ala Ile Asn T rp Ile Glu Gly Arg Gly Lys Ser Val 325 330 335 Val Ala Glu Ala Thr Ile Pro Gly Asp Val Val Arg Lys Val Leu Lys 340 345 350 Ser Asp Val Ser Ala Leu Val Glu Leu Asn Ile Ala Lys Asn Leu Val 355 360 365 Gly Ser Ala Met Ala Gly Ser Val Gly Gly Phe Asn Ala His Ala Ala 370 375 380 Asn Leu Val Thr Ala Val Phe Leu Ala Leu Gly Gln Asp Pro Ala Gln 385 390 395 400 Asn Val Glu Ser Ser Asn cys Ile Thr Leu Met Lys Glu Val Asp Gly 405 410 415 Asp Leu Arg Ile Ser Val Ser Met Pro Ser Ile Glu Val Gly Thr Ile 420 425 430 Gly Gly Gly Thr Val Leu Gl u Pro Gln Gly Ala Met Leu Asp Leu Leu 435 440 445 Gly Val Arg Gly Pro His Ala Thr Ala Pro Gly Thr Asn Ala Arg Gln 450 455 460 Leu Ala Arg Ile Val Ala cys Ala Val Leu Ala Gly Glu Leu Ser Leu 465 470 475 480 cys Ala Ala Leu Ala Ala Gly His Leu Val Gln Ser His Met Thr His 485 490 495 Asn Arg Lys Pro Ala Glu Pro Thr Lys Pro Asn Asn Leu Asp Ala Thr 500 505 510 Asp Ile Asn Arg Leu Lys Asp Gly Ser Val Thr cys Ile Lys Ser 515 520 525

<210> 107 <211> 3681 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic Page 120

2018200459 19 Jan 2018 polynucleotide

<400> 107 atgagagctg tccttagatt gttatcaaca catactgttt tctctcctat tgaaacaatt 60 gtatctgttt tcgtgttagc tacattagct tacttccaca tcttgtccgg aatcaagcac 120 tcaagtttct ttgcatcttc tcatcctcct gctatcagac ctgcttttgc acatctgacc 180 aacggggaat gggttgccgt ctcccaacat gattggactg aagcatggaa gcatcctggc 240 ggttcacttg atgcattaga acttcaacaa gtagttttca ctttagatga caagactcaa 300 ccatctgctg tgctagatgc atccgcaatt agtcagcact tagtttccaa tgttcctgca 360 ttatctggaa aagcctactc ttcattgtgc caccatccaa atgtatcagg cacctcctgt 420 tttacatcag tttctggtcc aggagcttca ccaatcttga cactgagttt taagcctgga 480 actagagacg attggttagg atcattaagg aaggagaaaa ctatcacact agatggggtt 540 aagtacgacg ttggagccgg aaaaagacaa gagtcaatcg gcgatatgga atcatctaag 600 tgggttgctt atgcattatc agctttggta cttagatttt gggaattaac aaaggcagat 660 tccttagata tactagtggt tctaactggg tacatcctaa tgcacgtaac attcatgaga 720 ttgttcttgg catccagagc acttggcagt aacttttggt tatcagctgg catattctcc 780 tccgcaacaa tttctttcct attcacttta ccaatgtgta gatctatgga tattccactt 840 gatccaattg ccttgacaga agccctgcca ttcttggtgt gtaccgtagg ttttgacaaa 900 ccacttagat tggcaagagc tgtgatggct catcctaata tccttaaacc tcaagatgat 960 ggtaggatga aagctgccgg agatgtcatt cttgaggcac tggacagagt tggtaacatg 1020 atattgagag attacgcttt agagatcgca gttctattcg ttggcgttaa ctccagagtt 1080 ggcggtctta aggaattttg tgctgtagct gcagcattac ttgctatgga cagattaatg 1140 acattcacac tttatacagc agtgttaacc atcatggttg aggtaaggcg tatcaaaaag 1200 gtcagagata tgactaaggc tagatctaga agttcttcta ttaccgccgt tacagccaac 1260 ggcaccgcca taagaggcgt tttgagtaga aaatcttcaa aacaatctgt gacagaacca 1320 gagacaacta aaaacctaag acaaagagcc actgattcag ccatcggtgt taagggttca 1380 ttgctgaaag atggaggcag attgcaggaa gccgaggaga atccaatggc aagattaaag 1440 ctattgttaa tcgcttcctt cttaacacta cacatcttga acttttgtac tactttgact 1500 tcagccacag ctaacgcaag acatcaaaga catcctttta gaaccgttca agaggtagta 1560 ccaattccta gagttgacat tactacccca gccatagcca atatcttgtc tcatctagct 1620 gtggctcagg aacctatgtt cactgttgtt ggcagtgaac ctatcgaact tcttgttaaa 1680 gtcgctgctc cagtctacgt ccatgctcta ccattggccc ctgctttaag agcttcaaac 1740 actaatactg gagaagctat tgaaaacttt atgagttcat ggtctagtct ggtaggtgac 1800 ccagttgtta gtaagtggat cgtagcattg ctagctgtct ctgttgcatt gaatggatac 1860 ttgttaaagg gtatagccgc aggttccggg ttggctgcca tgagagctgt tagatctcaa 1920 ggtgttcgtt tcagatctag agctagaagt atcgtaaaga tatctgatga acctgagcca 1980

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gagccagaac actctatcga cccagcacca gtagtgttct tcgcttccgc agcaccagct 2040 gtagaggccc ctgctccagc tcctgcacct gaaccagaac caccagtcaa cagaccacca 2100 ccattgacta ttttctcaag accactgaac ttagaaacag tggacaaaaa gttacaagat 2160 gctctgccaa taagatcccc accacctgtt gaaccaatca ctccagaatc tagagaagtg 2220 gaaccaaccc aagtagaagt aagatctcta gctgaatgtg tggatgtgtt cgagaatggg 2280 ccaagaccag tctcagtggc tttaaagact ctgaatgatg aggaagttat cctgctttgc 2340 caaacaggta agatagctcc atatgcattg gttaagatgt tggctgattt cgatagggcc 2400 gtacgtgtca gaagagcact tattagtaga gcttcacgta caaaaacttt agaaaactca 2460 ctggttccta tgaaagatta tgattacgcc agagtcatgg gtgcctgttg tgaaaacgtt 2520 atcggataca tgccattacc actagggatt gcaggtccat tgaagattga tggcttgatg 2580 tatcctatac caatggcaac cgcagaaggt accttggttg catctacttc taggggctgt 2640 aaggccttaa atgctggtgg aggggtcaca actgtcttga cagcagatgg catgacaaga 2700 gggccagcta tagactttcc ttccatcgtc agagctgcag aggctaaggc cttcattgaa 2760 tcagaagatg gatacgctac aatcagggag gctttcgagt ctacttctag atttgccaag 2820 ttgcaaaaga tcaagtgtgc actagctggt cgtactcttt ttgtcagatt tgctactaga 2880 acaggagatg ccatgggtat gaacatgatt tctaaggcta ccgaaaaggc acttgatgtc 2940 ctgagtcacg agttccctga aatggtcgtc cttgctttgt ctggtaacta ctgcacagac 3000 aaaaagcctg cagctatttc atggatcgaa ggtaggggaa aatctattgt agcagaagca 3060 gttattcctg gtaaggtcgt taagtcagtc ctgaaaacaa cagtcgagtc tctttgcaat 3120 gtcaacacta agaaaaacct gattggttca gccatggcag gttctgttgg tggtttcaac 3180 gctcatgccg ccaacatcct aacagctgtg ttcctagcca caggtcagga tcctgctcaa 3240 aatgtcgaat cttctaattg catgacttta atggaaccaa caaacggcgg tgaggatttg 3300 ctaatgacaa tttcaatgcc atgtatagag gtaggaaccg ttggtggagg gacaattctg 3360 gaaccacaag gtgcagtttt ggatttgttg ggcgttagag gggctcaccc tactaatcct 3420 ggtcaaaacg ctcaacagtt agccagaatt atcgcatcag ctgtaatggc aggcgaattg 3480 tctttgataa gtgccttagc cgcaggtcat ttggttagag ctcatcttgc ccacaatcgt 3540 tctcaattga atacaccaat gccatccaga ccacatactc ctggccctga ggatgtctca 3600 catgtgcagc agctacctac accatctgca tctgatgata aaggtgttac agctcaaggt 3660 tacgttgtcg aagcaaaata a 3681

<210> 108 <211> 1226 <212> PRT <213> Ganoderma lucidum <400> 108

Met Arg Ala Val Leu Arg Leu Leu Ser Thr His Thr Val Phe Ser Pro 15 10 15

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Ile Glu Thr Ile Val Ser Val Phe Val 25 Leu Ala Thr Leu Ala Tyr 30 Phe 20 His Ile Leu Ser Gly Ile Lys His Ser Ser Phe Phe Ala Ser Ser His 35 40 45 Pro Pro Ala Ile Arg Pro Ala Phe Ala His Leu Thr Asn Gly Glu T rp 50 55 60 Val Ala Val Ser Gln His Asp T rp Thr Glu Ala T rp Lys His Pro Gly 65 70 75 80 Gly Ser Leu Asp Ala Leu Gl u Leu Gln Gln Val Val Phe Thr Leu Asp 85 90 95 Asp Lys Thr Gln Pro Ser Ala Val Leu Asp Ala Ser Ala Ile Ser Gln 100 105 110 His Leu Val Ser Asn Val Pro Ala Leu Ser Gly Lys Ala Tyr Ser Ser 115 120 125 Leu cys His His Pro Asn Val Ser Gly Thr Ser cys Phe Thr Ser Val 130 135 140 Ser Gly Pro Gly Ala Ser Pro Ile Leu Thr Leu Ser Phe Lys Pro Gly 145 150 155 160 Thr Arg Asp Asp T rp Leu Gly Ser Leu Arg Lys Glu Lys Thr Ile Thr 165 170 175 Leu Asp Gly Val Lys Tyr Asp Val Gly Ala Gly Lys Arg Gln Glu Ser 180 185 190 Ile Gly Asp Met Glu Ser Ser Lys T rp Val Ala Tyr Ala Leu Ser Ala 195 200 205 Leu Val Leu Arg Phe T rp Gl u Leu Thr Lys Ala Asp Ser Leu Asp Ile 210 215 220 Leu Val Val Leu Thr Gly Tyr Ile Leu Met His Val Thr Phe Met Arg 225 230 235 240 Leu Phe Leu Ala Ser Arg Ala Leu Gly Ser Asn Phe T rp Leu Ser Ala 245 250 255 Gly Ile Phe Ser Ser Ala Thr Ile Ser Phe Leu Phe Thr Leu Pro Met 260 265 270 cys Arg Ser Met Asp Ile Pro Leu Asp Pro Ile Ala Leu Thr Glu Ala 275 280 285 Leu Pro Phe Leu Val cys Thr Val Gly Phe Asp Lys Pro Leu Arg Leu Page 123

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290 295 300

Ala 305 Arg Ala Val Met Ala 310 Hi s Pro Asn Ile Leu 315 Lys Pro Gln Asp Asp 320 Gly Arg Met Lys Ala Ala Gly Asp Val Ile Leu Glu Ala Leu Asp Arg 325 330 335 Val Gly Asn Met Ile Leu Arg Asp Tyr Ala Leu Glu Ile Ala Val Leu 340 345 350 Phe Val Gly Val Asn Ser Arg Val Gly Gly Leu Lys Glu Phe cys Ala 355 360 365 Val Ala Ala Ala Leu Leu Ala Met Asp Arg Leu Met Thr Phe Thr Leu 370 375 380 Tyr Thr Ala Val Leu Thr Ile Met Val Glu Val Arg Arg Ile Lys Lys 385 390 395 400 Val Arg Asp Met Thr Lys Ala Arg Ser Arg Ser Ser Ser Ile Thr Ala 405 410 415 Val Thr Ala Asn Gly Thr Ala Ile Arg Gly Val Leu Ser Arg Lys Ser 420 425 430 Ser Lys Gln Ser Val Thr Gl u Pro Glu Thr Thr Lys Asn Leu Arg Gln 435 440 445 Arg Ala Thr Asp Ser Ala Ile Gly Val Lys Gly Ser Leu Leu Lys Asp 450 455 460 Gly Gly Arg Leu Gln Glu Ala Glu Glu Asn Pro Met Ala Arg Leu Lys 465 470 475 480 Leu Leu Leu Ile Ala Ser Phe Leu Thr Leu His Ile Leu Asn Phe cys 485 490 495 Thr Thr Leu Thr Ser Ala Thr Ala Asn Ala Arg His Gln Arg His Pro 500 505 510 Phe Arg Thr Val Gln Glu Val Val Pro Ile Pro Arg Val Asp Ile Thr 515 520 525 Thr Pro Ala Ile Ala Asn Ile Leu Ser His Leu Ala Val Ala Gln Glu 530 535 540 Pro Met Phe Thr Val Val Gly Ser Glu Pro Ile Glu Leu Leu Val Lys 545 550 555 560 Val Ala Ala Pro Val Tyr Val His Ala Leu Pro Leu Ala Pro Ala Leu 565 570 575

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Arg Ala Ser Asn 580 Thr Asn Thr Gly Glu Ala Ile 585 Glu Asn Phe 590 Met Ser Ser T rp Ser Ser Leu Val Gly Asp Pro Val Val Ser Lys T rp Ile Val 595 600 605 Ala Leu Leu Ala Val Ser Val Ala Leu Asn Gly Tyr Leu Leu Lys Gly 610 615 620 Ile Ala Ala Gly Ser Gly Leu Ala Ala Met Arg Ala Val Arg Ser Gln 625 630 635 640 Gly Val Arg Phe Arg Ser Arg Ala Arg Ser Ile Val Lys Ile Ser Asp 645 650 655 Glu Pro Glu Pro Glu Pro Gl u His Ser Ile Asp Pro Ala Pro Val Val 660 665 670 Phe Phe Ala Ser Ala Ala Pro Ala Val Glu Ala Pro Ala Pro Ala Pro 675 680 685 Ala Pro Glu Pro Glu Pro Pro Val Asn Arg Pro Pro Pro Leu Thr Ile 690 695 700 Phe Ser Arg Pro Leu Asn Leu Glu Thr Val Asp Lys Lys Leu Gln Asp 705 710 715 720 Ala Leu Pro Ile Arg Ser Pro Pro Pro Val Glu Pro Ile Thr Pro Glu 725 730 735 Ser Arg Glu Val Glu Pro Thr Gln Val Glu Val Arg Ser Leu Ala Glu 740 745 750 cys Val Asp Val Phe Glu Asn Gly Pro Arg Pro Val Ser Val Ala Leu 755 760 765 Lys Thr Leu Asn Asp Glu Gl u Val Ile Leu Leu cys Gln Thr Gly Lys 770 775 780 Ile Ala Pro Tyr Ala Leu Val Lys Met Leu Ala Asp Phe Asp Arg Ala 785 790 795 800 Val Arg Val Arg Arg Ala Leu Ile Ser Arg Ala Ser Arg Thr Lys Thr 805 810 815 Leu Glu Asn Ser Leu Val Pro Met Lys Asp Tyr Asp Tyr Ala Arg Val 820 825 830 Met Gly Ala cys cys Glu Asn Val Ile Gly Tyr Met Pro Leu Pro Leu 835 840 845

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Gly Ile Ala Gly Pro Leu Lys Ile Asp Gly Leu Met Tyr Pro Ile Pro 850 855 860 Met Ala Thr Ala Glu Gly Thr Leu Val Ala Ser Thr Ser Arg Gly cys 865 870 875 880 Lys Ala Leu Asn Ala Gly Gly Gly Val Thr Thr Val Leu Thr Ala Asp 885 890 895 Gly Met Thr Arg Gly Pro Ala Ile Asp Phe Pro Ser Ile Val Arg Ala 900 905 910 Ala Glu Ala Lys Ala Phe Ile Glu Ser Glu Asp Gly Tyr Ala Thr Ile 915 920 925 Arg Glu Ala Phe Glu Ser Thr Ser Arg Phe Ala Lys Leu Gln Lys Ile 930 935 940 Lys cys Ala Leu Ala Gly Arg Thr Leu Phe Val Arg Phe Ala Thr Arg 945 950 955 960 Thr Gly Asp Ala Met Gly Met Asn Met Ile Ser Lys Ala Thr Glu Lys 965 970 975 Ala Leu Asp Val Leu Ser His Glu Phe Pro Glu Met Val Val Leu Ala 980 985 990 Leu Ser Gly Asn Tyr cys Thr Asp Lys Lys Pro Alt 1 Al i Ile Ser Trp

995 1000 1005

Ile Glu 1010 Gly Arg Gly Lys Ser 1015 Ile Val Ala Glu Ala 1020 Val Ile Pro Gly Lys Val Val Lys Ser Val Leu Lys Thr Thr Val Glu Ser Leu 1025 1030 1035 cys Asn Val Asn Thr Lys Lys Asn Leu Ile Gly Ser Ala Met Ala 1040 1045 1050 Gly Ser Val Gly Gly Phe Asn Ala His Ala Ala Asn Ile Leu Thr 1055 1060 1065 Ala Val Phe Leu Ala Thr Gly Gln Asp Pro Ala Gln Asn Val Glu 1070 1075 1080 Ser Ser Asn cys Met Thr Leu Met Glu Pro Thr Asn Gly Gly Glu 1085 1090 1095 Asp Leu Leu Met Thr Ile Ser Met Pro cys Ile Glu Val Gly Thr 1100 1105 1110

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Val Gly 1115 Gly Gly Thr Ile Leu 1120 Glu Pro Gln Gly Ala 1125 Val Leu Asp Leu Leu Gly Val Arg Gly Ala His Pro Thr Asn Pro Gly Gln Asn 1130 1135 1140 Ala Gln Gln Leu Ala Arg Ile Ile Ala Ser Ala Val Met Ala Gly 1145 1150 1155 Glu Leu Ser Leu Ile Ser Ala Leu Ala Ala Gly His Leu Val Arg 1160 1165 1170 Ala His Leu Ala His Asn Arg Ser Gln Leu Asn Thr Pro Met Pro 1175 1180 1185 Ser Arg Pro His Thr Pro Gly Pro Glu Asp Val Ser His Val Gln 1190 1195 1200 Gln Leu Pro Thr Pro Ser Ala Ser Asp Asp Lys Gly Val Thr Ala 1205 1210 1215 Gln Gly Tyr Val Val Glu Ala Lys 1220 1225

<210> 109 <211> 2667 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 109 atgttatcaa gattgttcag aatgcatggt ctatttgttg cttctcaccc ttgggaagta 60 atagttggta ctgtaacatt aacgatctgt atgatgtcta tgaacatgtt taccggaaac 120 aacaagattt gtggttggaa ttatgagtgt cctaagctgg aagaggatgt gttgagttca 180 gacatcatca tacttactat aacaagatgc attgcaatat tgtatatcta cttccaattt 240 caaaacctta gacaattggg tagtaaatac atcctaggca tcgccggatt gttcactatt 300 ttctctagtt ttgttttctc aaccgtcgtt attcactttt tggacaaaga gttaactggt 360 ttgaacgaag ctctaccatt cttcttgctg ctggtagatt tgtccagagc ttccgcttta 420 gctaaattcg ctctgtcctc taattctcaa gatgaagtta gagagaatat agcaagggga 480 atggccatac ttggacctac tttcacactt gatgcccttg tcgaatgttt ggttattggg 540 gttggcacaa tgtccggcgt tagacagtta gaaatcatgt gttgttttgg ctgtatgagt 600 gtcttggcta actactttgt ctttatgaca ttctttccag cttgcgtttc tttggtattg 660 gagctgtcaa gagaatcaag agaaggcaga ccaatatggc aactatcaca tttcgccaga 720 gtgttagaag aggaggaaaa caaacctaat cctgtcacac agagagtgaa aatgatcatg 780 tctttgggtt tagtcctagt gcatgctcat tctagatgga tcgcagatcc atcccctcag 840

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aattctacag ctgataactc taaagttagt ttaggtttag atgaaaatgt aagtaagagg 900 attgaacctt ccgtgtcttt gtggcaattc tacttatcaa aaatgatttc catggatatt 960 gaacaagtga taacgttgtc tttggcttta ttgttagccg ttaagtacat tttctttgag 1020 caagccgaaa cggaatctac attatcactg aaaaacccaa ttacatcccc agtcgttacc 1080 cagaaaaaga taactgatga ttgctgtaga agagatccag tgttggtcag gaatgatcaa 1140 aagttccacg ccatggagga ggaaactagg aaaaacagag aaaggaaagt tgaagttatc 1200 aagcctctat tagcagaaaa tgacacttca catagggcca ctttcgttgt cggcaattca 1260 tctcttttag gtacgtcatt ggagctggaa acacaggaac cagaaatgga actaccagtt 1320 gaaccaagac caaatgagga atgtttgcaa atactagaga acgctgaaaa gggagccaag 1380 ttcctatctg atgccgagat tatccagctg gtcaatgcca agcacattcc tgcctacaag 1440 ttggaaaccc ttatggagac acatgagaga ggtgtgtcta ttaggagaca attactatct 1500 aaaaagttac ctgaaccaag ttccctacaa tacctgcctt atagagatta caattactcc 1560 ttggtaatgg gagcttgttg tgaaaatgtc attgggtaca tgccaattcc agtgggtgtc 1620 gccggtccac tatgtttgga cggtaaggaa tttcaagtac ctatggcaac gactgaaggc 1680 tgcttagttg catctacaaa cagaggttgt agagccattg gattaggtgg cggtgcttct 1740 tcaagagtct tggctgacgg tatgactaga ggtcctgttg tgagatttcc tagggcctgt 1800 gactctgcag aagttaaggc ttggttggaa actccagaag gtttcaccgt aatcaaagag 1860 gcctttgatt ccacatcaag ggtggccaga ttacaaaaac tacacatgtc tgtcgctggg 1920 agaaatctgt atatcagatt tcaatccaga tccggcgacg caatgggtat gaatatgatt 1980 tcaaaaggga cagaaaaggc tttgtcaaag ctgcaggagt atttcccaga gatgcaaatc 2040 ttggccgtat ctggcaacta ttgcacagac aaaaagcctg ccgccatcaa ctggattgaa 2100 ggaagaggca aatctgtggt ttgtgaagct gtaattccag ccaaagttgt tagagaagtg 2160 ttaaagacca caacagaagc tatgattgaa gtaaacataa acaaaaactt agtagggtct 2220 gccatggctg gttcaattgg aggatacaac gctcatgctg ccaatattgt aaccgctatc 2280 tacatcgcat gtggacaaga tgctgcccaa aatgtcggtt cctcaaattg catcacattg 2340 atggaagcat ctggccctac aaacgaggat ttgtatatca gttgcacaat gccatctata 2400 gaaataggga ctgtgggagg aggaactaac ttacttccac agcaagcctg cttacaaatg 2460 ctgggtgtac aaggagcctg tagagataat ccaggggaga acgctagaca acttgccaga 2520 attgtttgtg ggacagttat ggctggtgaa cttagtctaa tggcagcttt ggctgctggg 2580 cacctggtga gatctcatat gattcataat agaagtaaga ttaaccttca agatttgcaa 2640 ggtacgtgta cgaaaaaggc tgcctaa 2667

<210> 110 <211> 888 <212> PRT <213> Bos taurus

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2018200459 19 Jan 2018 <400> 110

Met 1 Leu Ser Arg Leu 5 Phe Arg Met His Gly Leu 10 Phe Val Ala Ser 15 His Pro T rp Glu Val Ile Val Gly Thr Val Thr Leu Thr Ile cys Met Met 20 25 30 Ser Met Asn Met Phe Thr Gly Asn Asn Lys Ile cys Gly T rp Asn Tyr 35 40 45 Glu cys Pro Lys Leu Glu Gl u Asp Val Leu Ser Ser Asp Ile Ile Ile 50 55 60 Leu Thr Ile Thr Arg cys Ile Ala Ile Leu Tyr Ile Tyr Phe Gln Phe 65 70 75 80 Gln Asn Leu Arg Gln Leu Gly Ser Lys Tyr Ile Leu Gly Ile Ala Gly 85 90 95 Leu Phe Thr Ile Phe Ser Ser Phe Val Phe Ser Thr Val Val Ile His 100 105 110 Phe Leu Asp Lys Glu Leu Thr Gly Leu Asn Glu Ala Leu Pro Phe Phe 115 120 125 Leu Leu Leu Val Asp Leu Ser Arg Ala Ser Ala Leu Ala Lys Phe Ala 130 135 140 Leu Ser Ser Asn Ser Gln Asp Glu Val Arg Glu Asn Ile Ala Arg Gly 145 150 155 160 Met Ala Ile Leu Gly Pro Thr Phe Thr Leu Asp Ala Leu Val Glu cys 165 170 175 Leu Val Ile Gly Val Gly Thr Met Ser Gly Val Arg Gln Leu Glu Ile 180 185 190 Met cys cys Phe Gly cys Met Ser Val Leu Ala Asn Tyr Phe Val Phe 195 200 205 Met Thr Phe Phe Pro Ala cys Val Ser Leu Val Leu Glu Leu Ser Arg 210 215 220 Glu Ser Arg Glu Gly Arg Pro Ile T rp Gln Leu Ser His Phe Ala Arg 225 230 235 240 Val Leu Glu Glu Glu Glu Asn Lys Pro Asn Pro Val Thr Gln Arg Val 245 250 255 Lys Met Ile Met Ser Leu Gly Leu Val Leu Val His Ala His Ser Arg 260 265 270

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Trp Ile

Val Ser 290

Val Ser 305

Glu Gln

Ile Phe

Pro Ile

Cys Arg 370

Met Glu 385

Lys Pro

Val Gly

Glu Pro

Leu Gln 450

Ala Glu 465

Leu Glu

Gln Leu

Pro Tyr

Asn Val 530

Cys Leu

Ala Asp 275

Leu Gly

Leu Trp

Val Ile

Phe Glu 340

Thr Ser 355

Arg Asp

Glu Glu

Leu Leu

Asn Ser 420

Glu Met 435

Ile Leu

Ile Ile

Thr Leu

Leu Ser 500

Arg Asp 515

Ile Gly

Asp Gly

Pro Ser

Leu Asp

Gln Phe 310

Thr Leu 325

Gln Ala

Pro Val

Pro Val

Thr Arg 390

Ala Glu 405

Ser Leu

Glu Leu

Glu Asn

Gln Leu 470

Met Glu 485

Lys Lys

Tyr Asn

Tyr Met

Lys Glu

Pro Gln 280

Glu Asn 295

Tyr Leu

Ser Leu

Glu Thr

Val Thr 360

Leu Val 375

Lys Asn

Asn Asp

Leu Gly

Pro Val 440

Ala Glu 455

Val Asn

Thr His

Leu Pro

Tyr Ser 520

Pro Ile 535

Phe Gln

Asn Ser

Val Ser

Ser Lys

Ala Leu 330

Glu Ser 345

Gln

Arg

Arg

Thr

Thr 425

Glu

Lys

Ala

Glu

Glu 505

Leu

Pro

Val

Thr

Lys Lys

Asn Asp

Glu Arg 395

Ser His 410

Ser Leu

Pro Arg

Gly Ala

Lys His 475

Arg Gly 490

Pro Ser

Val Met

Val Gly

Pro Met

Page 130

Thr Ala

Lys Arg 300

Met Ile 315

Leu Leu

Leu

Ile

Gln 380

Lys

Arg

Glu

Pro

Lys 460

Ile

Val

Ser

Gly

Val 540

Ala

Asp Asn 285

Ile Glu

Ser Met

Ala Val

Ser Leu 350

Thr Asp 365

Lys Phe

Val Glu

Ala Thr

Leu Glu 430

Asn Glu 445

Phe Leu

Pro Ala

Ser Ile

Leu Gln 510

Ala Cys 525

Ala Gly

Thr Thr

Ser Lys

Pro Ser

Asp Ile 320

Lys Tyr 335

Lys Asn

Asp Cys

His Ala

Val Ile 400

Phe Val 415

Thr Gln

Glu Cys

Ser Asp

Tyr Lys 480

Arg Arg 495

Tyr Leu

Cys Glu

Pro Leu

Glu Gly

2018200459 19 Jan 2018

545 550 555 560

cys Leu Val Ala Ser 565 Thr Asn Arg Gly cys Arg Ala Ile Gly Leu Gly 570 575 Gly Gly Ala Ser Ser Arg Val Leu Ala Asp Gly Met Thr Arg Gly Pro 580 585 590 Val Val Arg Phe Pro Arg Ala cys Asp Ser Ala Glu Val Lys Ala T rp 595 600 605 Leu Glu Thr Pro Glu Gly Phe Thr Val Ile Lys Glu Ala Phe Asp Ser 610 615 620 Thr Ser Arg Val Ala Arg Leu Gln Lys Leu His Met Ser Val Ala Gly 625 630 635 640 Arg Asn Leu Tyr Ile Arg Phe Gln Ser Arg Ser Gly Asp Ala Met Gly 645 650 655 Met Asn Met Ile Ser Lys Gly Thr Glu Lys Ala Leu Ser Lys Leu Gln 660 665 670 Glu Tyr Phe Pro Glu Met Gl n Ile Leu Ala Val Ser Gly Asn Tyr cys 675 680 685 Thr Asp Lys Lys Pro Ala Ala Ile Asn T rp Ile Glu Gly Arg Gly Lys 690 695 700 Ser Val Val cys Glu Ala Val Ile Pro Ala Lys Val Val Arg Glu Val 705 710 715 720 Leu Lys Thr Thr Thr Glu Ala Met Ile Glu Val Asn Ile Asn Lys Asn 725 730 735 Leu Val Gly Ser Ala Met Ala Gly Ser Ile Gly Gly Tyr Asn Ala His 740 745 750 Ala Ala Asn Ile Val Thr Ala Ile Tyr Ile Ala cys Gly Gln Asp Ala 755 760 765 Ala Gln Asn Val Gly Ser Ser Asn cys Ile Thr Leu Met Glu Ala Ser 770 775 780 Gly Pro Thr Asn Glu Asp Leu Tyr Ile Ser cys Thr Met Pro Ser Ile 785 790 795 800 Glu Ile Gly Thr Val Gly Gly Gly Thr Asn Leu Leu Pro Gln Gln Ala 805 810 815 cys Leu Gln Met Leu Gly Val Gln Gly Ala cys Arg Asp Asn Pro Gly 820 825 830

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Glu Asn Ala 835 Arg Gln Leu Ala Arg 840 Ile Val cys Gly Thr 845 Val Met Ala Gly Glu Leu Ser Leu Met Ala Ala Leu Ala Ala Gly His Leu Val Arg 850 855 860 Ser His Met Ile His Asn Arg Ser Lys Ile Asn Leu Gln Asp Leu Gln 865 870 875 880 Gly Thr cys Thr Lys Lys Ala Ala

885 <210> 111 <211> 1704 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 111

atggatttga gaaggaaatt accacctaag cctccatctt caacaacaac aaaacagcca 60 agtcataggt cccattctcc tacgccaatt ccaaaggctt cagatgcatt gcctcttcca 120 ttgtacctga ccaatacgtt tttcttcact cttttctttt ccgtagcata ttacctgttg 180 cataggtgga gagacaagat tagatccgga acacctttac acgttgtgac actgactgaa 240 ctatccgcaa ttgtactgct gattgcttcc ttcatctatc ttttaggctt tttcggtatt 300 gattttgtgc aatctttcac atcaagagaa aatgagcaac taaacaacga tgatcacaac 360 gtcgtgtcaa caaacaatgt tttatctgat agaaggttag tttacgacta tggattcgat 420 gtgacaggag acaacgataa cgataatgat gacgatgtta ttgtgaaaag tgtcgtttct 480 ggggaagtta attcttatag tttggaggct tccctaggag attgttacag agccgcaaag 540 attagaaaga gagccgtcga gagaattgtc gggagagaag tattaggctt gggtttcgag 600 ggatttgatt atgaatctat cctggggcaa tgttgtgaaa tgcctatcgg gtacgtccaa 660 gtgccagtag gtgtcgctgg acctttattg ttaaatggtg gggaattcat ggttccaatg 720 gctacaactg aaggctgtct tgtagcttcc actaatagag gttgtaaagc catatgctta 780 tcaggtggtg ccactgccat attgctaaaa gatggtatga caagagcccc agtagtgaga 840 ttcgccacag ctgagagagc ttcacaacta aagttttact tggaagatgg tgtcaatttc 900 gatacattgt ctgttgtctt taacaaaagt tcaagatttg ccagattgca aaacatccaa 960 tgctcaattg ccggtaaaaa cttgtacatt aggtttactt gctccacagg cgacgccatg 1020 ggtatgaaca tggtttcaaa aggagtacaa aatgtattag actttttaca aaatgatttt 1080 cctgatatgg acgtaattgg gatctcttgg aagttctgct ctgacaaaaa gccaacagct 1140 gtcaactgga ttgagggcag aggaaagtct gtcgttttcc aggccgtaat taccaaaaag 1200 gtggttagaa agtctgcact gaaccctcaa acttgcacat gtagaacttt gacctgttta 1260

Page 132

2018200459 19 Jan 2018 agaccattat atcgtgtctg cactgtatca atgccatcta tgtttgaact ttgttagcta ataagtgctg tcagcaatag tggttctgct ccgtgttcat ctatgatgga tagaagttgg tgcttggtgt gaatcgttgc ggcaactagt cttctaaggt acttctggtt cgctaccggt ggctgtcaac cacggtggga aaagggtgcc tggttctgtt taaatctcat ctaa ttgctagtgg caagatccag aatggtaagg ggtggcactc tgtatagaat ctggcaggcg atgaaataca acttaatgca ctcagaatat atttgcacgt agctagcctc ccccaggatc aattaagttt ataggtctag tatgcttcat cgaatctagt taatgttacg tcaatcagcc aaacgcccag gatgtcagct tagagatatg

1320

1380

1440

1500

1560

1620

1680

1704

<210> 112 <211> 567 <212> PRT <213> Artemisia annua <400> 112

Met 1 Asp Leu Arg Arg 5 Lys Leu Pro Pro Lys 10 Pro Pro Ser Ser Thr 15 Thr Thr Lys Gln Pro Ser His Arg Ser His Ser Pro Thr Pro Ile Pro Lys 20 25 30 Ala Ser Asp Ala Leu Pro Leu Pro Leu Tyr Leu Thr Asn Thr Phe Phe 35 40 45 Phe Thr Leu Phe Phe Ser Val Ala Tyr Tyr Leu Leu His Arg T rp Arg 50 55 60 Asp Lys Ile Arg Ser Gly Thr Pro Leu His Val Val Thr Leu Thr Glu 65 70 75 80 Leu Ser Ala Ile Val Leu Leu Ile Ala Ser Phe Ile Tyr Leu Leu Gly 85 90 95 Phe Phe Gly Ile Asp Phe Val Gln Ser Phe Thr Ser Arg Glu Asn Glu 100 105 110 Gln Leu Asn Asn Asp Asp His Asn Val Val Ser Thr Asn Asn Val Leu 115 120 125 Ser Asp Arg Arg Leu Val Tyr Asp Tyr Gly Phe Asp Val Thr Gly Asp 130 135 140 Asn Asp Asn Asp Asn Asp Asp Asp Val Ile Val Lys Ser Val Val Ser 145 150 155 160 Gly Glu Val Asn Ser Tyr Ser Leu Glu Ala Ser Leu Gly Asp cys Tyr 165 170 175 Arg Ala Ala Lys Ile Arg Lys Arg Ala Val Glu Arg Ile Val Gly Arg

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180 185 190 Glu Val Leu Gly Leu Gly Phe Glu Gly Phe Asp Tyr Glu Ser Ile Leu 195 200 205 Gly Gln cys cys Glu Met Pro Ile Gly Tyr Val Gln Val Pro Val Gly 210 215 220 Val Ala Gly Pro Leu Leu Leu Asn Gly Gly Glu Phe Met Val Pro Met 225 230 235 240 Ala Thr Thr Glu Gly cys Leu Val Ala Ser Thr Asn Arg Gly cys Lys 245 250 255 Ala Ile cys Leu Ser Gly Gly Ala Thr Ala Ile Leu Leu Lys Asp Gly 260 265 270 Met Thr Arg Ala Pro Val Val Arg Phe Ala Thr Ala Glu Arg Ala Ser 275 280 285 Gln Leu Lys Phe Tyr Leu Gl u Asp Gly Val Asn Phe Asp Thr Leu Ser 290 295 300 Val Val Phe Asn Lys Ser Ser Arg Phe Ala Arg Leu Gln Asn Ile Gln 305 310 315 320 cys Ser Ile Ala Gly Lys Asn Leu Tyr Ile Arg Phe Thr cys Ser Thr 325 330 335 Gly Asp Ala Met Gly Met Asn Met Val Ser Lys Gly Val Gln Asn Val 340 345 350 Leu Asp Phe Leu Gln Asn Asp Phe Pro Asp Met Asp Val Ile Gly Ile 355 360 365 Ser T rp Lys Phe cys Ser Asp Lys Lys Pro Thr Ala Val Asn T rp Ile 370 375 380 Glu Gly Arg Gly Lys Ser Val Val Phe Gln Ala Val Ile Thr Lys Lys 385 390 395 400 Val Val Arg Lys Ser Ala Leu Asn Pro Gln Thr cys Thr cys Arg Thr 405 410 415 Leu Thr cys Leu Arg Pro Leu Leu Val Leu Leu Leu Leu Val Leu Leu 420 425 430 Val Asp Leu Met His Met Leu His Ile Val Ser Ala Val Phe Ile Ala 435 440 445 Thr Gly Gln Asp Pro Ala Gl n Asn Ile Glu Ser Ser His cys Ile Thr

450 455 460

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Met 465 Met Glu Ala Val Asn 470 Asn Gly Lys Asp Leu 475 His Val Asn Val Thr 480 Met Pro Ser Ile Glu Val Gly Thr Val Gly Gly Gly Thr Gln Leu Ala 485 490 495 Ser Gln Ser Ala cys Leu Asn Leu Leu Gly Val Lys Gly Ala cys Ile 500 505 510 Glu Ser Pro Gly Ser Asn Ala Gln Leu Leu Ala Arg Ile Val Ala Gly 515 520 525 Ser Val Leu Ala Gly Glu Leu Ser Leu Met Ser Ala Ile Ser Ala Gly 530 535 540 Gln Leu Val Lys Ser His Met Lys Tyr Asn Arg Ser Ser Arg Asp Met 545 550 555 560 Ser Ala Ile Ala Ser Lys Val

565 <210> 113 <211> 1308 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 113 atgtttagaa gagctatact gttaggatgc tctgctgcca agacaccatg gtctgagtgt 60 tctaacgctc aattagttga tgcagttaag tctagaaaga tctcattcta cggtcttgaa 120 caagccttgg aaccagatta tagaagggct atcgaagtaa ggagagaggt tgtctctgaa 180 atcgcctcac aacagccaga agcaaaaaag aagcaatccg cattgcacac aataccattt 240 gagaattatg attggaataa ggtcgttggc caaaactgtg aaaacattat tggatacgtc 300 ccaataccac tgggcgttgc tggccctatt ttgattgatg gtaaagagta cccaatacca 360 atggctacaa cagaaggcgc tttggtcgct agtactcata gaggtgctag agctattaca 420 agatccggag gttgtaagac attgttatta ggtgaaggta tgacaagagc accagtggtt 480 gaattgcctt cattagagga agctgggcgt ttgcacaagt actgtaatga gaacttctta 540 tctttaaagg aagcatttga atcaactacc caatatggaa aacttaattc tttaaagtgc 600 gtactagctg gtagaaaagc ataccttaga ttcagagcca ctacaggcga tgctatgggc 660 atgaacatga taacaaaggg tgtagacaaa gcactgtctg ttctacagca acatttccct 720 tcaatggaaa tcctagccct aagtggtaat tactgtaccg acaaaaagcc atctgctgta 780 aattggattg atggcagagg taaatcagtg gttgcagaag ccactttatt ggctgatgtt 840 gtcgaagata ctctgaaatg tacagtcgat tctttggtat ccttgaatat cgacaaaaac 900

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2018200459 19 Jan 2018

cttgttgggt cagctatggc tggttctgtt ggaggtttta acgcccaggc tgcaaacgct 960 gtggcagcca ttttcattgc aaccggtcaa gatcctgctc aagtggtaga aagttcaatg 1020 tgtatcacta caatgtccaa ggtaggtaac gatctattga tctctgtgac catgccttct 1080 atcgaggtcg gggtcgtggg aggagggact ggtcttgctg cccaaagagg atgcttagag 1140 ttaatagggt gcggaggccc atctaaggag tctcctggta ctaatgccca acttctaagt 1200 agagttgttg cagctggcgt tttatcagcc gaactttcct tgatgtccgg actggcagca 1260 ggtcatctat tgtcagcaca tatgagattg aacagaaaga agaaataa 1308

<210> 114 <211> 435 <212> PRT <213> Trypanosoma cruzi

<400> 114 Met 1 Phe Arg Arg Ala Ile 5 Leu Leu Gly cys 10 Ser Ala Ala Lys Thr 15 Pro T rp Ser Glu cys Ser Asn Ala Gln Leu Val Asp Ala Val Lys Ser Arg 20 25 30 Lys Ile Ser Phe Tyr Gly Leu Glu Gln Ala Leu Glu Pro Asp Tyr Arg 35 40 45 Arg Ala Ile Glu Val Arg Arg Glu Val Val Ser Glu Ile Ala Ser Gln 50 55 60 Gln Pro Glu Ala Lys Lys Lys Gln Ser Ala Leu His Thr Ile Pro Phe 65 70 75 80 Glu Asn Tyr Asp T rp Asn Lys Val Val Gly Gln Asn cys Glu Asn Ile 85 90 95 Ile Gly Tyr Val Pro Ile Pro Leu Gly Val Ala Gly Pro Ile Leu Ile 100 105 110 Asp Gly Lys Glu Tyr Pro Ile Pro Met Ala Thr Thr Glu Gly Ala Leu 115 120 125 Val Ala Ser Thr His Arg Gly Ala Arg Ala Ile Thr Arg Ser Gly Gly 130 135 140 cys Lys Thr Leu Leu Leu Gly Glu Gly Met Thr Arg Ala Pro Val Val 145 150 155 160 Glu Leu Pro Ser Leu Glu Gl u Ala Gly Arg Leu His Lys Tyr cys Asn 165 170 175 Glu Asn Phe Leu Ser Leu Lys Glu Ala Phe Glu Ser Thr Thr Gln Tyr 180 185 190

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Gly Lys Leu Asn Ser Leu Lys cys Val Leu Ala Gly Arg Lys Ala Tyr 195 200 205 Leu Arg Phe Arg Ala Thr Thr Gly Asp Ala Met Gly Met Asn Met Ile 210 215 220 Thr Lys Gly Val Asp Lys Ala Leu Ser Val Leu Gln Gln His Phe Pro 225 230 235 240 Ser Met Glu Ile Leu Ala Leu Ser Gly Asn Tyr cys Thr Asp Lys Lys 245 250 255 Pro Ser Ala Val Asn T rp Ile Asp Gly Arg Gly Lys Ser Val Val Ala 260 265 270 Glu Ala Thr Leu Leu Ala Asp Val Val Glu Asp Thr Leu Lys cys Thr 275 280 285 Val Asp Ser Leu Val Ser Leu Asn Ile Asp Lys Asn Leu Val Gly Ser 290 295 300 Ala Met Ala Gly Ser Val Gly Gly Phe Asn Ala Gln Ala Ala Asn Ala 305 310 315 320 Val Ala Ala Ile Phe Ile Ala Thr Gly Gln Asp Pro Ala Gln Val Val 325 330 335 Glu Ser Ser Met cys Ile Thr Thr Met Ser Lys Val Gly Asn Asp Leu 340 345 350 Leu Ile Ser Val Thr Met Pro Ser Ile Glu Val Gly Val Val Gly Gly 355 360 365 Gly Thr Gly Leu Ala Ala Gl n Arg Gly cys Leu Glu Leu Ile Gly cys 370 375 380 Gly Gly Pro Ser Lys Glu Ser Pro Gly Thr Asn Ala Gln Leu Leu Ser 385 390 395 400 Arg Val Val Ala Ala Gly Val Leu Ser Ala Glu Leu Ser Leu Met Ser 405 410 415 Gly Leu Ala Ala Gly His Leu Leu Ser Ala His Met Arg Leu Asn Arg

420

Lys Lys Lys 435 <210> 115 <211> 1281 <212> DNA <213> Artificial Sequence

425

430

Page 137

2018200459 19 Jan 2018 <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de <400> 115

atgcaatccc tggacaaaaa ctttagacac ttatcaagac aacagaagtt acaacagcta 60 gttgataaac aatggctatc agaggaacaa ttcaatattc tacttaacca cccacttatt 120 gatgaagagg tagcaaactc attgatagaa aatgtcatcg cacagggcgc actgcctgtt 180 ggtttactac caaatatcat cgttgatgac aaagcatacg tcgtgcctat gatggtggaa 240 gagccatctg ttgttgccgc tgcttcatac ggcgctaaat tggtgaacca aacaggtggt 300 ttcaaaaccg tgtcctcaga acgtatcatg ataggtcaaa tagtatttga tggagtcgat 360 gataccgaga aactgtctgc agatatcaag gctcttgaaa aacaaatcca tcagattgca 420 gatgaggctt acccttctat taaggccaga ggtggaggct atcaaaggat cgccatcgat 480 acattcccag aacaacagtt gctttcattg aaggttttcg ttgatactaa ggatgctatg 540 ggcgctaata tgttaaacac aatcctagaa gcaatcacag cctttttgaa aaacgaattc 600 ccacaatctg atatcttgat gtctatcctt tccaaccacg caacagccag tgttgtcaag 660 gtccagggtg aaatagacgt taaggatttg gcaagaggag aacgtactgg agaagaggtc 720 gctaagagaa tggaaagagc atctgtgtta gctcaagtgg acattcatag agcagcaaca 780 cacaataagg gtgttatgaa tggcattcat gctgtagtct tggctacagg taatgatact 840 agaggtgcag aagcctctgc tcacgcttac gcttccaaag acggtcaata tagagggata 900 gctacatgga gatacgatca agagagacaa aggttaatag gaactataga agttccaatg 960 actctggcca ttgttggtgg cggtaccaag gtactgccta ttgctaaggc ctctttagaa 1020 ctgttaaacg tagaaagtgc ccaagagttg ggacatgttg tcgctgccgt tggactagct 1080 caaaacttcg ctgcatgtag agctttggtt tccgaaggta ttcaacaagg gcatatgtct 1140 ttgcaataca agtctttagc catcgtagtc ggggctaagg gcgatgaaat tgctcaggta 1200 gccgaagcac taaagcaaga gccaagagca aacactcaag ttgcagagag aattttgcaa 1260 gatttgagaa gtcaacaata a 1281

<210> 116 <211> 426 <212> PRT <213> Staphylococcus aureus <400> 116

Met 1 Gln Ser Leu Asp 5 Lys Asn Phe Arg His 10 Leu Ser Arg Gln Gln 15 Lys Leu Gln Gln Leu Val Asp Lys Gln T rp Leu Ser Glu Glu Gln Phe Asn 20 25 30 Ile Leu Leu Asn His Pro Leu Ile Asp Glu Glu Val Ala Asn Ser Leu

35 40 45

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Ile Glu Asn Val Ile Ala Gl n Gly Ala Leu Pro Val Gly Leu Leu Pro 50 55 60 Asn Ile Ile Val Asp Asp Lys Ala Tyr Val Val Pro Met Met Val Glu 65 70 75 80 Glu Pro Ser Val Val Ala Ala Ala Ser Tyr Gly Ala Lys Leu Val Asn 85 90 95 Gln Thr Gly Gly Phe Lys Thr Val Ser Ser Glu Arg Ile Met Ile Gly 100 105 110 Gln Ile Val Phe Asp Gly Val Asp Asp Thr Glu Lys Leu Ser Ala Asp 115 120 125 Ile Lys Ala Leu Glu Lys Gl n Ile His Gln Ile Ala Asp Glu Ala Tyr 130 135 140 Pro Ser Ile Lys Ala Arg Gly Gly Gly Tyr Gln Arg Ile Ala Ile Asp 145 150 155 160 Thr Phe Pro Glu Gln Gln Leu Leu Ser Leu Lys Val Phe Val Asp Thr 165 170 175 Lys Asp Ala Met Gly Ala Asn Met Leu Asn Thr Ile Leu Glu Ala Ile 180 185 190 Thr Ala Phe Leu Lys Asn Gl u Phe Pro Gln Ser Asp Ile Leu Met Ser 195 200 205 Ile Leu Ser Asn His Ala Thr Ala Ser Val Val Lys Val Gln Gly Glu 210 215 220 Ile Asp Val Lys Asp Leu Ala Arg Gly Glu Arg Thr Gly Glu Glu Val 225 230 235 240 Ala Lys Arg Met Glu Arg Ala Ser Val Leu Ala Gln Val Asp Ile His 245 250 255 Arg Ala Ala Thr His Asn Lys Gly Val Met Asn Gly Ile His Ala Val 260 265 270 Val Leu Ala Thr Gly Asn Asp Thr Arg Gly Ala Glu Ala Ser Ala His 275 280 285 Ala Tyr Ala Ser Lys Asp Gly Gln Tyr Arg Gly Ile Ala Thr T rp Arg 290 295 300 Tyr Asp Gln Glu Arg Gln Arg Leu Ile Gly Thr Ile Glu Val Pro Met 305 310 315 320 Thr Leu Ala Ile Val Gly Gly Gly Thr Lys Val Leu Pro Ile Ala Lys

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325

330

335

Ala Ser Leu Glu Leu Leu Asn Val Glu Ser Ala Gln Glu Leu Gly His 340 345 350 Val Val Ala Ala Val Gly Leu Ala Gln Asn Phe Ala Ala cys Arg Ala 355 360 365 Leu Val Ser Glu Gly Ile Gl n Gln Gly His Met Ser Leu Gln Tyr Lys 370 375 380 Ser Leu Ala Ile Val Val Gly Ala Lys Gly Asp Glu Ile Ala Gln Val 385 390 395 400 Ala Glu Ala Leu Lys Gln Gl u Pro Arg Ala Asn Thr Gln Val Ala Glu 405 410 415 Arg Ile Leu Gln Asp Leu Arg Ser Gln Gln 420 425

<210> 117 <211> 1311 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic polynucleoti de

<400> 117 atgcaggtct taagattgga taggagacat tacaaaagtg gcaagattag aagagcaatg 60 agttctagaa ttcctggttt ctacaaattg tcagtcgagg aaagactgaa aaaggttgct 120 gaatttgcag ggttatctga tgaggaagtg aaagctgttt tgtcacaagg tttacctttg 180 gacgtagctg atagaatgat cgaaaatgtg atcggtacat ttgaattacc acttggtata 240 gcaaccaatt tccttattga tggcaaggat tatctaatcc ctatggctat agaggaacca 300 tcagtagttg cagctgcttc taacgcagct agaatggcca gagagtctgg cgggtttaca 360 actgattaca cagggtccct gatgattggt caaattcaag tcacaaaact gttgaatcca 420 aatgcagcta agttcgaagt tctacgtcaa aaagacgaaa tcatagaaag agcaaatgag 480 tgtgatccaa tgttggtgaa tttgggcggt ggatgtaaag atatagaagc aagggtgatc 540 gatacaatca tgggtaagat gctaattgtt catctgatcg ttgatgttaa agacgctatg 600 ggtgcaaatg ctgtcaacac tatgtgtgaa aaagttgctc ctttcatcga acgtattact 660 gggggaaagg tctatcttag aatcatttcc aacttggctg catatagact tgctagagca 720 aaggccgttt ttgacaaaga cgttattggc ggagaggagg ttgtagaagg gatcatgctt 780 gcatacgcct tcgctgccgc tgacccattt cgttgcgcca cccacaataa gggtatcatg 840 aatggcatat cagccttaat gatcgctaca ggaaacgact ttagagccat tgaagcagga 900 gctcattcct atgctgcaat aggtggatac aaaccactaa ctacctacga agttgataga 960

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2018200459 19 Jan 2018 aaaggtaatc tagtaggcac aattgaaata cctatggcag taggcgtgat tggtggtgca accaaagtca acccactagc caagatctct cttaagatac taggagtgaa cactgctgaa gagttagcca gagtcgcagc cgctctaggt ttggctcaaa actttgctgc cttaagagcc ttggccacag aaggtatcca aagaggtcac atggaattac atgccaggaa cttagcaatc atggctggag ctactggaga tgaggttgac agagttgtag agattatggt gagagatggc aaaatcagat tggactacgc taaggaagta ttggagagac tgcgttccta a <210> 118 <211> 436 <212> PRT <213> Archaeoglobus fulgidus

<400> 118 Asp Arg Arg His 10 Tyr Lys Ser Gly Lys 15 Ile Met 1 Gln Val Leu Arg 5 Leu Arg Arg Ala Met Ser Ser Arg Ile Pro Gly Phe Tyr Lys Leu Ser Val 20 25 30 Glu Glu Arg Leu Lys Lys Val Ala Glu Phe Ala Gly Leu Ser Asp Glu 35 40 45 Glu Val Lys Ala Val Leu Ser Gln Gly Leu Pro Leu Asp Val Ala Asp 50 55 60 Arg Met Ile Glu Asn Val Ile Gly Thr Phe Glu Leu Pro Leu Gly Ile 65 70 75 80 Ala Thr Asn Phe Leu Ile Asp Gly Lys Asp Tyr Leu Ile Pro Met Ala 85 90 95 Ile Glu Glu Pro Ser Val Val Ala Ala Ala Ser Asn Ala Ala Arg Met 100 105 110 Ala Arg Glu Ser Gly Gly Phe Thr Thr Asp Tyr Thr Gly Ser Leu Met 115 120 125 Ile Gly Gln Ile Gln Val Thr Lys Leu Leu Asn Pro Asn Ala Ala Lys 130 135 140 Phe Glu Val Leu Arg Gln Lys Asp Glu Ile Ile Glu Arg Ala Asn Glu 145 150 155 160 cys Asp Pro Met Leu Val Asn Leu Gly Gly Gly cys Lys Asp Ile Glu 165 170 175 Ala Arg Val Ile Asp Thr Ile Met Gly Lys Met Leu Ile Val His Leu 180 185 190 Ile Val Asp Val Lys Asp Ala Met Gly Ala Asn Ala Val Asn Thr Met 195 200 205

1020

1080

1140

1200

1260

1311

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2018200459 19 Jan 2018

cys Glu Lys Val 210 Ala Pro Phe Ile Glu 215 Arg Ile Thr 220 Gly Gly Lys Val Tyr Leu Arg Ile Ile Ser Asn Leu Ala Ala Tyr Arg Leu Ala Arg Ala 225 230 235 240 Lys Ala Val Phe Asp Lys Asp Val Ile Gly Gly Glu Glu Val Val Glu 245 250 255 Gly Ile Met Leu Ala Tyr Ala Phe Ala Ala Ala Asp Pro Phe Arg cys 260 265 270 Ala Thr His Asn Lys Gly Ile Met Asn Gly Ile Ser Ala Leu Met Ile 275 280 285 Ala Thr Gly Asn Asp Phe Arg Ala Ile Glu Ala Gly Ala His Ser Tyr 290 295 300 Ala Ala Ile Gly Gly Tyr Lys Pro Leu Thr Thr Tyr Glu Val Asp Arg 305 310 315 320 Lys Gly Asn Leu Val Gly Thr Ile Glu Ile Pro Met Ala Val Gly Val 325 330 335 Ile Gly Gly Ala Thr Lys Val Asn Pro Leu Ala Lys Ile Ser Leu Lys 340 345 350 Ile Leu Gly Val Asn Thr Ala Glu Glu Leu Ala Arg Val Ala Ala Ala 355 360 365 Leu Gly Leu Ala Gln Asn Phe Ala Ala Leu Arg Ala Leu Ala Thr Glu 370 375 380 Gly Ile Gln Arg Gly His Met Glu Leu His Ala Arg Asn Leu Ala Ile 385 390 395 400 Met Ala Gly Ala Thr Gly Asp Glu Val Asp Arg Val Val Glu Ile Met 405 410 415 Val Arg Asp Gly Lys Ile Arg Leu Asp Tyr Ala Lys Glu Val Leu Glu

420 425 430

Arg Leu Arg Ser 435 <210> 119 <211> 1287 <212> DNA <213> Artificial Sequence <220>

<223> Description of Artificial Sequence: Synthetic Page 142

2018200459 19 Jan 2018 polynucleotide

<400> 119 atgtccttag attcaagact gccagctttc agaaatctgt ctccagctgc aagactagat 60 cacattggcc aacttttggg actaagtcat gacgacgttt cccttttagc aaacgccggt 120 gctttaccaa tggatatcgc taatggtatg attgaaaatg taatcgggac ctttgaactg 180 ccatatgcag tggccagtaa ctttcagatc aatggccgtg acgtcttagt accattagtt 240 gtggaggaac ctagtatcgt tgctgcagcc tcttacatgg caaagttagc tagagccaat 300 ggtgggttca ctacatcttc atctgctcca ctaatgcatg cacaagtaca aattgtcggc 360 attcaggatc cactaaacgc aagattgtct ttactgcgta gaaaggatga gatcatagaa 420 ttagccaata ggaaggacca acttctgaat tcattgggcg gtggttgcag agacatagag 480 gtgcatacat ttgccgatac tccaagagga ccaatgcttg tagcacacct tattgtcgat 540 gtgcgtgatg ccatgggagc taatactgtt aacactatgg ctgaagcagt agcacctctg 600 atggaagcca taacaggtgg ccaggtaaga ttgagaatcc tttccaattt ggctgatctt 660 agattggcca gagcccaagt gagaatcact cctcagcaat tggaaactgc cgaattctca 720 ggtgaggcag taattgaggg tatcttggac gcatatgctt ttgccgctgt ggacccttac 780 agagccgcta cccacaacaa aggcataatg aacggtatcg atcctttgat cgtcgctaca 840 ggaaatgatt ggagagctgt tgaggcagga gctcatgcat acgcttgtag atccggacat 900 tacggttcat taacaacatg ggaaaaagat aacaatggac acttggtcgg gacattggaa 960 atgcctatgc cagttggttt agttgggggt gctacaaaaa cccatcctct tgctcaattg 1020 tctttgagga tacttggtgt caaaactgct caagcactag ccgaaattgc cgttgctgtt 1080 ggtttggcac aaaacttggg tgcaatgcgt gctttagcta cagaaggcat ccaaagagga 1140 catatggctc tacacgctag aaacattgca gttgttgcag gagccagagg tgatgaggtt 1200 gattgggtgg ctagacaact tgtcgaatat catgatgtca gagcagacag ggctgtggca 1260 ttactgaaac agaagagagg tcaataa 1287

<210> 120 <211> 428 <212> PRT <213> Pseudomonas mevalonii <400> 120

Met 1 Ser Leu Asp Ser 5 Arg Leu Pro Ala Phe Arg Asn 10 Leu Ser Pro 15 Ala Ala Arg Leu Asp His Ile Gly Gln Leu Leu Gly Leu Ser His Asp Asp 20 25 30 Val Ser Leu Leu Ala Asn Ala Gly Ala Leu Pro Met Asp Ile Ala Asn 35 40 45 Gly Met Ile Glu Asn Val Ile Gly Thr Phe Glu Leu Pro Tyr Ala Val 50 55 60

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Ala 65 Ser Asn Phe Gln Ile 70 Asn Gly Arg Asp Val 75 Leu Val Pro Leu Val 80 Val Glu Glu Pro Ser Ile Val Ala Ala Ala Ser Tyr Met Ala Lys Leu 85 90 95 Ala Arg Ala Asn Gly Gly Phe Thr Thr Ser Ser Ser Ala Pro Leu Met 100 105 110 His Ala Gln Val Gln Ile Val Gly Ile Gln Asp Pro Leu Asn Ala Arg 115 120 125 Leu Ser Leu Leu Arg Arg Lys Asp Glu Ile Ile Glu Leu Ala Asn Arg 130 135 140 Lys Asp Gln Leu Leu Asn Ser Leu Gly Gly Gly cys Arg Asp Ile Glu 145 150 155 160 Val His Thr Phe Ala Asp Thr Pro Arg Gly Pro Met Leu Val Ala His 165 170 175 Leu Ile Val Asp Val Arg Asp Ala Met Gly Ala Asn Thr Val Asn Thr 180 185 190 Met Ala Glu Ala Val Ala Pro Leu Met Glu Ala Ile Thr Gly Gly Gln 195 200 205 Val Arg Leu Arg Ile Leu Ser Asn Leu Ala Asp Leu Arg Leu Ala Arg 210 215 220 Ala Gln Val Arg Ile Thr Pro Gln Gln Leu Glu Thr Ala Glu Phe Ser 225 230 235 240 Gly Glu Ala Val Ile Glu Gly Ile Leu Asp Ala Tyr Ala Phe Ala Ala 245 250 255 Val Asp Pro Tyr Arg Ala Ala Thr His Asn Lys Gly Ile Met Asn Gly 260 265 270 Ile Asp Pro Leu Ile Val Ala Thr Gly Asn Asp T rp Arg Ala Val Glu 275 280 285 Ala Gly Ala His Ala Tyr Ala cys Arg Ser Gly His Tyr Gly Ser Leu 290 295 300 Thr Thr T rp Glu Lys Asp Asn Asn Gly His Leu Val Gly Thr Leu Glu 305 310 315 320 Met Pro Met Pro Val Gly Leu Val Gly Gly Ala Thr Lys Thr His Pro 325 330 335

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Leu Ala Gln Leu 340 Ser Leu Arg Ile Leu Gly Val 345 Lys Thr Ala Gln Ala 350 Leu Ala Glu Ile Ala Val Ala Val Gly Leu Ala Gln Asn Leu Gly Ala 355 360 365 Met Arg Ala Leu Ala Thr Gl u Gly Ile Gln Arg Gly His Met Ala Leu 370 375 380 His Ala Arg Asn Ile Ala Val Val Ala Gly Ala Arg Gly Asp Glu Val 385 390 395 400 Asp T rp Val Ala Arg Gln Leu Val Glu Tyr His Asp Val Arg Ala Asp 405 410 415 Arg Ala Val Ala Leu Leu Lys Gln Lys Arg Gly Gln

420 425 <210> 121 <211> 361 <212> PRT <213> Stevia rebaudiana <400> 121

Met Ala 1 Leu Val Asn 5 Pro Thr Ala Leu Phe Tyr Gly Thr Ser 10 Ile 15 Arg Thr Arg Pro Thr Asn Leu Leu Asn Pro Thr Gln Lys Leu Arg Pro Val 20 25 30 Ser Ser Ser Ser Leu Pro Ser Phe Ser Ser Val Ser Ala Ile Leu Thr 35 40 45 Glu Lys His Gln Ser Asn Pro Ser Glu Asn Asn Asn Leu Gln Thr His 50 55 60 Leu Glu Thr Pro Phe Asn Phe Asp Ser Tyr Met Leu Glu Lys Val Asn 65 70 75 80 Met Val Asn Glu Ala Leu Asp Ala Ser Val Pro Leu Lys Asp Pro Ile 85 90 95 Lys Ile His Glu Ser Met Arg Tyr Ser Leu Leu Ala Gly Gly Lys Arg 100 105 110 Ile Arg Pro Met Met cys Ile Ala Ala cys Glu Ile Val Gly Gly Asn 115 120 125 Ile Leu Asn Ala Met Pro Ala Ala cys Ala Val Glu Met Ile His Thr 130 135 140 Met Ser Leu Val His Asp Asp Leu Pro cys Met Asp Asn Asp Asp Phe 145 150 155 160

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Arg Arg Gly Lys Pro Ile Ser 165 His Lys Val 170 Tyr Gly Glu Glu Met 175 Ala Val Leu Thr Gly Asp Ala Leu Leu Ser Leu Ser Phe Glu His Ile Ala 180 185 190 Thr Ala Thr Lys Gly Val Ser Lys Asp Arg Ile Val Arg Ala Ile Gly 195 200 205 Glu Leu Ala Arg Ser Val Gly Ser Glu Gly Leu Val Ala Gly Gln Val 210 215 220 Val Asp Ile Leu Ser Glu Gly Ala Asp Val Gly Leu Asp His Leu Glu 225 230 235 240 Tyr Ile His Ile His Lys Thr Ala Met Leu Leu Glu Ser Ser Val Val 245 250 255 Ile Gly Ala Ile Met Gly Gly Gly Ser Asp Gln Gln Ile Glu Lys Leu 260 265 270 Arg Lys Phe Ala Arg Ser Ile Gly Leu Leu Phe Gln Val Val Asp Asp 275 280 285 Ile Leu Asp Val Thr Lys Ser Thr Glu Glu Leu Gly Lys Thr Ala Gly 290 295 300 Lys Asp Leu Leu Thr Asp Lys Thr Thr Tyr Pro Lys Leu Leu Gly Ile 305 310 315 320 Glu Lys Ser Arg Glu Phe Ala Glu Lys Leu Asn Lys Glu Ala Gln Glu 325 330 335 Gln Leu Ser Gly Phe Asp Arg Arg Lys Ala Ala Pro Leu Ile Ala Leu 340 345 350 Ala Asn Tyr Asn Ala Tyr Arg Gln Asn

355 360 <210> 122 <211> 342 <212> PRT <213> Gibberella fujikuroi <400> 122

Met Ala Glu Gln 1 Gln Ile Ser Asn 5 Leu Leu Ser Met 10 Phe Asp Ala 15 Ser His Ala Ser Gln Lys Leu Gl u Ile Thr Val Gln Met Met Asp Thr Tyr 20 25 30 His Tyr Arg Glu Thr Pro Pro Asp Ser Ser Ser Ser Glu Gly Gly Ser 35 40 45

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Leu Ser Arg 50 Tyr Asp Glu Arg Arg 55 Val Ser Leu Pro 60 Leu Ser His Asn Ala Ala Ser Pro Asp Ile Val Ser Gln Leu cys Phe Ser Thr Ala Met 65 70 75 80 Ser Ser Glu Leu Asn His Arg T rp Lys Ser Gln Arg Leu Lys Val Ala 85 90 95 Asp Ser Pro Tyr Asn Tyr Ile Leu Thr Leu Pro Ser Lys Gly Ile Arg 100 105 110 Gly Ala Phe Ile Asp Ser Leu Asn Val T rp Leu Glu Val Pro Glu Asp 115 120 125 Glu Thr Ser Val Ile Lys Gl u Val Ile Gly Met Leu His Asn Ser Ser 130 135 140 Leu Ile Ile Asp Asp Phe Gl n Asp Asn Ser Pro Leu Arg Arg Gly Lys 145 150 155 160 Pro Ser Thr His Thr Val Phe Gly Pro Ala Gln Ala Ile Asn Thr Ala 165 170 175 Thr Tyr Val Ile Val Lys Ala Ile Glu Lys Ile Gln Asp Ile Val Gly 180 185 190 His Asp Ala Leu Ala Asp Val Thr Gly Thr Ile Thr Thr Ile Phe Gln 195 200 205 Gly Gln Ala Met Asp Leu Trp T rp Thr Ala Asn Ala Ile Val Pro Ser 210 215 220 Ile Gln Glu Tyr Leu Leu Met Val Asn Asp Lys Thr Gly Ala Leu Phe 225 230 235 240 Arg Leu Ser Leu Glu Leu Leu Ala Leu Asn Ser Glu Ala Ser Ile Ser 245 250 255 Asp Ser Ala Leu Glu Ser Leu Ser Ser Ala Val Ser Leu Leu Gly Gln 260 265 270 Tyr Phe Gln Ile Arg Asp Asp Tyr Met Asn Leu Ile Asp Asn Lys Tyr 275 280 285 Thr Asp Gln Lys Gly Phe cys Glu Asp Leu Asp Glu Gly Lys Tyr Ser 290 295 300 Leu Thr Leu Ile His Ala Leu Gln Thr Asp Ser Ser Asp Leu Leu Thr 305 310 315 320

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Asn Ile Leu Ser Met Arg Arg Val Gln Gly Lys Leu Thr Ala Gln Lys 325 330 335

Arg cys Trp Phe Trp Lys 340 <210> 123 <211> 300 <212> PRT <213> Mus musculus

<400> 123 Lys 5 Glu Lys Ala Glu Arg 10 Ile Leu Leu Glu Pro 15 Tyr Met 1 Glu Lys Thr Arg Tyr Leu Leu Gln Leu Pro Gly Lys Gln Val Arg Ser Lys Leu Ser 20 25 30 Gln Ala Phe Asn His T rp Leu Lys Val Pro Glu Asp Lys Leu Gln Ile 35 40 45 Ile Ile Glu Val Thr Glu Met Leu His Asn Ala Ser Leu Leu Ile Asp 50 55 60 Asp Ile Glu Asp Ser Ser Lys Leu Arg Arg Gly Phe Pro Val Ala His 65 70 75 80 Ser Ile Tyr Gly Val Pro Ser Val Ile Asn Ser Ala Asn Tyr Val Tyr 85 90 95 Phe Leu Gly Leu Glu Lys Val Leu Thr Leu Asp His Pro Asp Ala Val 100 105 110 Lys Leu Phe Thr Arg Gln Leu Leu Glu Leu His Gln Gly Gln Gly Leu 115 120 125 Asp Ile Tyr T rp Arg Asp Thr Tyr Thr cys Pro Thr Glu Glu Glu Tyr 130 135 140 Lys Ala Met Val Leu Gln Lys Thr Gly Gly Leu Phe Gly Leu Ala Val 145 150 155 160 Gly Leu Met Gln Leu Phe Ser Asp Tyr Lys Glu Asp Leu Lys Pro Leu 165 170 175 Leu Asp Thr Leu Gly Leu Phe Phe Gln Ile Arg Asp Asp Tyr Ala Asn 180 185 190 Leu His Ser Lys Glu Tyr Ser Glu Asn Lys Ser Phe cys Glu Asp Leu 195 200 205 Thr Glu Gly Lys Phe Ser Phe Pro Thr Ile His Ala Ile T rp Ser Arg 210 215 220

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Pro 225 Glu Ser Thr Gln Val 230 Gln Asn Ile Leu Arg 235 Gln Arg Thr Glu Asn 240 Ile Asp Ile Lys Lys Tyr cys Val Gln Tyr Leu Glu Asp Val Gly Ser 245 250 255 Phe Ala Tyr Thr Arg His Thr Leu Arg Glu Leu Glu Ala Lys Ala Tyr 260 265 270 Lys Gln Ile Glu Ala cys Gly Gly Asn Pro Ser Leu Val Ala Leu Val 275 280 285 Lys His Leu Ser Lys Met Phe Thr Glu Glu Asn Lys

290 295 300 <210> 124 <211> 339 <212> PRT

<213> Thalassiosira pseudonana <400> 124 Met Ala Arg Phe Tyr Phe Leu Asn Ala Leu Leu Met Val Ile Ser Leu 1 5 10 15 Gln Ser Thr Thr Ala Phe Thr Pro Ala Lys Leu Ala Tyr Pro Thr Thr 20 25 30 Thr Thr Ala Leu Asn Val Ala Ser Ala Glu Thr Ser Phe Ser Leu Asp 35 40 45 Glu Tyr Leu Ala Ser Lys Ile Gly Pro Ile Glu Ser Ala Leu Glu Ala 50 55 60 Ser Val Lys Ser Arg Ile Pro Gln Thr Asp Lys Ile cys Glu Ser Met 65 70 75 80 Ala Tyr Ser Leu Met Ala Gly Gly Lys Arg Ile Arg Pro Val Leu cys 85 90 95 Ile Ala Ala cys Glu Met Phe Gly Gly Ser Gln Asp Val Ala Met Pro 100 105 110 Thr Ala Val Ala Leu Glu Met Ile His Thr Met Ser Leu Ile His Asp 115 120 125 Asp Leu Pro Ser Met Asp Asn Asp Asp Leu Arg Arg Gly Lys Pro Thr 130 135 140 Asn His Val Val Phe Gly Glu Asp Val Ala Ile Leu Ala Gly Asp Ser 145 150 155 160 Leu Leu Ser Thr Ser Phe Glu His Val Ala Arg Glu Thr Lys Gly Val

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165 170 175 Ser Ala Glu Lys Ile Val Asp Val Ile Ala Arg Leu Gly Lys Ser Val 180 185 190 Gly Ala Glu Gly Leu Ala Gly Gly Gln Val Met Asp Leu Glu cys Glu 195 200 205 Ala Lys Pro Gly Thr Thr Leu Asp Asp Leu Lys T rp Ile His Ile His 210 215 220 Lys Thr Ala Thr Leu Leu Gl n Val Ala Val Ala Ser Gly Ala Val Leu 225 230 235 240 Gly Gly Ala Thr Pro Glu Gl u Val Ala Ala cys Glu Leu Phe Ala Met 245 250 255 Asn Ile Gly Leu Ala Phe Gl n Val Ala Asp Asp Ile Leu Asp Val Thr 260 265 270 Ala Ser Ser Glu Asp Leu Gly Lys Thr Ala Gly Lys Asp Glu Ala Thr 275 280 285 Asp Lys Thr Thr Tyr Pro Lys Leu Leu Gly Leu Glu Glu Ser Lys Ala 290 295 300 Tyr Ala Arg Gln Leu Ile Asp Glu Ala Lys Glu Ser Leu Ala Pro Phe 305 310 315 320 Gly Asp Arg Ala Ala Pro Leu Leu Ala Ile Ala Asp Phe Ile Ile Asp 325 330 335 Arg Lys Asn

<210> 125 <211> 355 <212> PRT <213> Streptomyces clavuligerus <400> 125

Met 1 His Leu Ala Pro 5 Arg Arg Val Pro Arg Gly Arg Arg Ser Pro Pro 10 15 Asp Arg Val Pro Glu Arg Gl n Gly Ala Leu Gly Arg Arg Arg Gly Ala 20 25 30 Gly Ser Thr Gly cys Ala Arg Ala Ala Ala Gly Val His Arg Arg Arg 35 40 45 Gly Gly Gly Glu Ala Asp Pro Ser Ala Ala Val His Arg Gly T rp Gln 50 55 60

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Ala Gly Gly 65 Gly Thr Gly Leu 70 Pro Asp Glu Val 75 Val Ser Thr Ala Ala 80 Ala Leu Glu Met Phe His Ala Phe Ala Leu Ile His Asp Asp Ile Met 85 90 95 Asp Asp Ser Ala Thr Arg Arg Gly Ser Pro Thr Val His Arg Ala Leu 100 105 110 Ala Asp Arg Leu Gly Ala Ala Leu Asp Pro Asp Gln Ala Gly Gln Leu 115 120 125 Gly Val Ser Thr Ala Ile Leu Val Gly Asp Leu Ala Leu Thr T rp Ser 130 135 140 Asp Glu Leu Leu Tyr Ala Pro Leu Thr Pro His Arg Leu Ala Ala Val 145 150 155 160 Leu Pro Leu Val Thr Ala Met Arg Ala Glu Thr Val His Gly Gln Tyr 165 170 175 Leu Asp Ile Thr Ser Ala Arg Arg Pro Gly Thr Asp Thr Ser Leu Ala 180 185 190 Leu Arg Ile Ala Arg Tyr Lys Thr Ala Ala Tyr Thr Met Glu Arg Pro 195 200 205 Leu His Ile Gly Ala Ala Leu Ala Gly Ala Arg Pro Glu Leu Leu Ala 210 215 220 Gly Leu Ser Ala Tyr Ala Leu Pro Ala Gly Glu Ala Phe Gln Leu Ala 225 230 235 240 Asp Asp Leu Leu Gly Val Phe Gly Asp Pro Arg Arg Thr Gly Lys Pro 245 250 255 Asp Leu Asp Asp Leu Arg Gly Gly Lys His Thr Val Leu Val Ala Leu 260 265 270 Ala Arg Glu His Ala Thr Pro Glu Gln Arg His Thr Leu Asp Thr Leu 275 280 285 Leu Gly Thr Pro Gly Leu Asp Arg Gln Gly Ala Ser Arg Leu Arg cys 290 295 300 Val Leu Val Ala Thr Gly Ala Arg Ala Glu Ala Glu Arg Leu Ile Thr 305 310 315 320 Glu Arg Arg Asp Gln Ala Leu Thr Ala Leu Asn Ala Leu Thr Leu Pro 325 330 335 Pro Pro Leu Ala Glu Ala Leu Ala Arg Leu Thr Leu Gly Ser Thr Ala Page 151

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His Pro Ala 355

340 345 350 <210> 126 <211> 330 <212> PRT <213> Sulfulobus acidicaldarius

<400> 126 Phe Asp Asn 5 Tyr Phe Asn Glu 10 Ile Val Asn Ser Val 15 Asn Met 1 Ser Tyr Asp Ile Ile Lys Ser Tyr Ile Ser Gly Asp Val Pro Lys Leu Tyr Glu 20 25 30 Ala Ser Tyr His Leu Phe Thr Ser Gly Gly Lys Arg Leu Arg Pro Leu 35 40 45 Ile Leu Thr Ile Ser Ser Asp Leu Phe Gly Gly Gln Arg Glu Arg Ala 50 55 60 Tyr Tyr Ala Gly Ala Ala Ile Glu Val Leu His Thr Phe Thr Leu Val 65 70 75 80 His Asp Asp Ile Met Asp Gl n Asp Asn Ile Arg Arg Gly Leu Pro Thr 85 90 95 Val His Val Lys Tyr Gly Leu Pro Leu Ala Ile Leu Ala Gly Asp Leu 100 105 110 Leu His Ala Lys Ala Phe Gl n Leu Leu Thr Gln Ala Leu Arg Gly Leu 115 120 125 Pro Ser Glu Thr Ile Ile Lys Ala Phe Asp Ile Phe Thr Arg Ser Ile 130 135 140 Ile Ile Ile Ser Glu Gly Gl n Ala Val Asp Met Glu Phe Glu Asp Arg 145 150 155 160 Ile Asp Ile Lys Glu Gln Gl u Tyr Leu Asp Met Ile Ser Arg Lys Thr 165 170 175 Ala Ala Leu Phe Ser Ala Ser Ser Ser Ile Gly Ala Leu Ile Ala Gly 180 185 190 Ala Asn Asp Asn Asp Val Arg Leu Met Ser Asp Phe Gly Thr Asn Leu 195 200 205 Gly Ile Ala Phe Gln Ile Val Asp Asp Ile Leu Gly Leu Thr Ala Asp 210 215 220

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Glu 225 Lys Glu Leu Gly Lys 230 Pro Val Lys Thr Ile Leu Val 245 Ile Lys Thr Lys Lys Ile Val 260 Leu Lys Ala Leu Glu Leu Met 275 Ser Ser Ala Asp Ile 280 Ala Tyr 290 Asn Leu Ala Glu Lys 295 Tyr Asn 305 Gln Val Ser Ser Lys 310 Ser Asp Leu Ala Glu Phe Thr Ile Arg Arg

325

Phe Ser Asp 235 Ile Arg Glu Gly Lys 240 Leu Glu 250 Leu cys Lys Glu Asp 255 Glu Gly 265 Asn Lys Ser Ala Ser 270 Lys Glu Ile Lys Lys Tyr Ser 285 Leu Asp Tyr Tyr Lys Asn Ala 300 Ile Asp Ser Leu Ile Pro Gly 315 Lys Ala Leu Lys Tyr 320

Arg Lys 330 <210> 127 <211> 297 <212> PRT

<213> Synechococcus sp. <400> 127 Gln Thr 5 Phe Asn Leu Met 1 Val Ala Gln Gln Val Glu 20 Glu Ala Leu Ser Glu Arg Ile 35 Tyr Glu Ala Met Arg 40 Arg Leu 50 Arg Pro Ile Leu cys 55 Leu Ser 65 Val Glu Gln Ala Met 70 Pro Thr Thr Met Ser Leu Ile 85 His Asp Asp Phe Arg Arg Gly 100 Lys Pro Thr Asn Ala Ile Leu 115 Ala Gly Asp Ala Leu 120 Ala Ser 130 Gln Thr Arg Gly Val 135 Pro

Asp Thr 10 Tyr Leu Ser Gln Arg 15 Gln Ala 25 Ala Leu Val Pro Ala 30 Tyr Pro Tyr Ser Leu Leu Ala 45 Gly Gly Lys Ala Ala cys Glu 60 Leu Ala Gly Gly Ala cys Ala 75 Leu Glu Met Ile His 80 Leu Pro 90 Ala Met Asp Asn Asp 95 Asp His 105 Lys Val Phe Gly Glu 110 Asp Ile Leu Ala Tyr Ala Phe 125 Glu His Ile Pro Gln Leu Val Leu Gln Val Ile

140

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Ala 145 Arg Ile Gly His Ala Val 150 Ala Ala Thr Gly 155 Leu Val Gly Gly Gln 160 Val Val Asp Leu Glu Ser Gl u Gly Lys Ala Ile Ser Leu Glu Thr Leu 165 170 175 Glu Tyr Ile His Ser His Lys Thr Gly Ala Leu Leu Glu Ala Ser Val 180 185 190 Val Ser Gly Gly Ile Leu Ala Gly Ala Asp Glu Glu Leu Leu Ala Arg 195 200 205 Leu Ser His Tyr Ala Arg Asp Ile Gly Leu Ala Phe Gln Ile Val Asp 210 215 220 Asp Ile Leu Asp Val Thr Ala Thr Ser Glu Gln Leu Gly Lys Thr Ala 225 230 235 240 Gly Lys Asp Gln Ala Ala Ala Lys Ala Thr Tyr Pro Ser Leu Leu Gly 245 250 255 Leu Glu Ala Ser Arg Gln Lys Ala Glu Glu Leu Ile Gln Ser Ala Lys 260 265 270 Glu Ala Leu Arg Pro Tyr Gly Ser Gln Ala Glu Pro Leu Leu Ala Leu 275 280 285 Ala Asp Phe Ile Thr Arg Arg Gln His

290 295 <210> 128 <211> 371 <212> PRT <213> Arabidopsis thaliana <400> 128

Met 1 Ala Ser Val Thr 5 Leu Gly Ser T rp Ile Val 10 Val His His His 15 Asn His His His Pro Ser Ser Ile Leu Thr Lys Ser Arg Ser Arg Ser cys 20 25 30 Pro Ile Thr Leu Thr Lys Pro Ile Ser Phe Arg Ser Lys Arg Thr Val 35 40 45 Ser Ser Ser Ser Ser Ile Val Ser Ser Ser Val Val Thr Lys Glu Asp 50 55 60 Asn Leu Arg Gln Ser Glu Pro Ser Ser Phe Asp Phe Met Ser Tyr Ile 65 70 75 80 Ile Thr Lys Ala Glu Leu Val Asn Lys Ala Leu Asp Ser Ala Val Pro 85 90 95

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Leu Arg Glu Pro 100 Leu Lys Ile His Glu 105 Ala Met Arg Tyr Ser 110 Leu Leu Ala Gly Gly Lys Arg Val Arg Pro Val Leu cys Ile Ala Ala cys Glu 115 120 125 Leu Val Gly Gly Glu Glu Ser Thr Ala Met Pro Ala Ala cys Ala Val 130 135 140 Glu Met Ile His Thr Met Ser Leu Ile His Asp Asp Leu Pro cys Met 145 150 155 160 Asp Asn Asp Asp Leu Arg Arg Gly Lys Pro Thr Asn His Lys Val Phe 165 170 175 Gly Glu Asp Val Ala Val Leu Ala Gly Asp Ala Leu Leu Ser Phe Ala 180 185 190 Phe Glu His Leu Ala Ser Ala Thr Ser Ser Asp Val Val Ser Pro Val 195 200 205 Arg Val Val Arg Ala Val Gly Glu Leu Ala Lys Ala Ile Gly Thr Glu 210 215 220 Gly Leu Val Ala Gly Gln Val Val Asp Ile Ser Ser Glu Gly Leu Asp 225 230 235 240 Leu Asn Asp Val Gly Leu Gl u His Leu Glu Phe Ile His Leu His Lys 245 250 255 Thr Ala Ala Leu Leu Glu Ala Ser Ala Val Leu Gly Ala Ile Val Gly 260 265 270 Gly Gly Ser Asp Asp Glu Ile Glu Arg Leu Arg Lys Phe Ala Arg cys 275 280 285 Ile Gly Leu Leu Phe Gln Val Val Asp Asp Ile Leu Asp Val Thr Lys 290 295 300 Ser Ser Lys Glu Leu Gly Lys Thr Ala Gly Lys Asp Leu Ile Ala Asp 305 310 315 320 Lys Leu Thr Tyr Pro Lys Ile Met Gly Leu Glu Lys Ser Arg Glu Phe 325 330 335 Ala Glu Lys Leu Asn Arg Gl u Ala Arg Asp Gln Leu Leu Gly Phe Asp 340 345 350 Ser Asp Lys Val Ala Pro Leu Leu Ala Leu Ala Asn Tyr Ile Ala Tyr

355 360 365

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Arg Gln Asn 370 <210> 129 <211> 787 <212> PRT <213> Stevia rebaudiana <400> 129

Met 1 Lys Thr Gly Phe 5 Ile Ser Pro Ala Thr 10 Val Phe His His Arg 15 Ile Ser Pro Ala Thr Thr Phe Arg His His Leu Ser Pro Ala Thr Thr Asn 20 25 30 Ser Thr Gly Ile Val Ala Leu Arg Asp Ile Asn Phe Arg cys Lys Ala 35 40 45 Val Ser Lys Glu Tyr Ser Asp Leu Leu Gln Lys Asp Glu Ala Ser Phe 50 55 60 Thr Lys T rp Asp Asp Asp Lys Val Lys Asp His Leu Asp Thr Asn Lys 65 70 75 80 Asn Leu Tyr Pro Asn Asp Gl u Ile Lys Glu Phe Val Glu Ser Val Lys 85 90 95 Ala Met Phe Gly Ser Met Asn Asp Gly Glu Ile Asn Val Ser Ala Tyr 100 105 110 Asp Thr Ala T rp Val Ala Leu Val Gln Asp Val Asp Gly Ser Gly Ser 115 120 125 Pro Gln Phe Pro Ser Ser Leu Glu T rp Ile Ala Asn Asn Gln Leu Ser 130 135 140 Asp Gly Ser T rp Gly Asp His Leu Leu Phe Ser Ala His Asp Arg Ile 145 150 155 160 Ile Asn Thr Leu Ala cys Val Ile Ala Leu Thr Ser T rp Asn Val His 165 170 175 Pro Ser Lys cys Glu Lys Gly Leu Asn Phe Leu Arg Glu Asn Ile cys 180 185 190 Lys Leu Glu Asp Glu Asn Ala Glu His Met Pro Ile Gly Phe Glu Val 195 200 205 Thr Phe Pro Ser Leu Ile Asp Ile Ala Lys Lys Leu Asn Ile Glu Val 210 215 220 Pro Glu Asp Thr Pro Ala Leu Lys Glu Ile Tyr Ala Arg Arg Asp Ile 225 230 235 240 Page 156

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Lys Leu Thr Lys Ile 245 Pro Met Glu Val Leu 250 His Lys Val Pro Thr 255 Thr Leu Leu His Ser Leu Glu Gly Met Pro Asp Leu Glu T rp Glu Lys Leu 260 265 270 Leu Lys Leu Gln cys Lys Asp Gly Ser Phe Leu Phe Ser Pro Ser Ser 275 280 285 Thr Ala Phe Ala Leu Met Gl n Thr Lys Asp Glu Lys cys Leu Gln Tyr 290 295 300 Leu Thr Asn Ile Val Thr Lys Phe Asn Gly Gly Val Pro Asn Val Tyr 305 310 315 320 Pro Val Asp Leu Phe Glu His Ile T rp Val Val Asp Arg Leu Gln Arg 325 330 335 Leu Gly Ile Ala Arg Tyr Phe Lys Ser Glu Ile Lys Asp cys Val Glu 340 345 350 Tyr Ile Asn Lys Tyr T rp Thr Lys Asn Gly Ile cys T rp Ala Arg Asn 355 360 365 Thr His Val Gln Asp Ile Asp Asp Thr Ala Met Gly Phe Arg Val Leu 370 375 380 Arg Ala His Gly Tyr Asp Val Thr Pro Asp Val Phe Arg Gln Phe Glu 385 390 395 400 Lys Asp Gly Lys Phe Val cys Phe Ala Gly Gln Ser Thr Gln Ala Val 405 410 415 Thr Gly Met Phe Asn Val Tyr Arg Ala Ser Gln Met Leu Phe Pro Gly 420 425 430 Glu Arg Ile Leu Glu Asp Ala Lys Lys Phe Ser Tyr Asn Tyr Leu Lys 435 440 445 Glu Lys Gln Ser Thr Asn Gl u Leu Leu Asp Lys T rp Ile Ile Ala Lys 450 455 460 Asp Leu Pro Gly Glu Val Gly Tyr Ala Leu Asp Ile Pro T rp Tyr Ala 465 470 475 480 Ser Leu Pro Arg Leu Glu Thr Arg Tyr Tyr Leu Glu Gln Tyr Gly Gly 485 490 495 Glu Asp Asp Val T rp Ile Gly Lys Thr Leu Tyr Arg Met Gly Tyr Val 500 505 510

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Ser Asn Asn 515 Thr Tyr Leu Gl u Met Ala Lys 520 Leu Asp Tyr Asn Asn Tyr 525 Val Ala Val Leu Gln Leu Gl u T rp Tyr Thr Ile Gln Gln T rp Tyr Val 530 535 540 Asp Ile Gly Ile Glu Lys Phe Glu Ser Asp Asn Ile Lys Ser Val Leu 545 550 555 560 Val Ser Tyr Tyr Leu Ala Ala Ala Ser Ile Phe Glu Pro Glu Arg Ser 565 570 575 Lys Glu Arg Ile Ala T rp Ala Lys Thr Thr Ile Leu Val Asp Lys Ile 580 585 590 Thr Ser Ile Phe Asp Ser Ser Gln Ser Ser Lys Glu Asp Ile Thr Ala 595 600 605 Phe Ile Asp Lys Phe Arg Asn Lys Ser Ser Ser Lys Lys His Ser Ile 610 615 620 Asn Gly Glu Pro T rp His Gl u Val Met Val Ala Leu Lys Lys Thr Leu 625 630 635 640 His Gly Phe Ala Leu Asp Ala Leu Met Thr His Ser Gln Asp Ile His 645 650 655 Pro Gln Leu His Gln Ala Trp Glu Met T rp Leu Thr Lys Leu Gln Asp 660 665 670 Gly Val Asp Val Thr Ala Gl u Leu Met Val Gln Met Ile Asn Met Thr 675 680 685 Ala Gly Arg T rp Val Ser Lys Glu Leu Leu Thr His Pro Gln Tyr Gln 690 695 700 Arg Leu Ser Thr Val Thr Asn Ser Val cys His Asp Ile Thr Lys Leu 705 710 715 720 His Asn Phe Lys Glu Asn Ser Thr Thr Val Asp Ser Lys Val Gln Glu 725 730 735 Leu Val Gln Leu Val Phe Ser Asp Thr Pro Asp Asp Leu Asp Gln Asp 740 745 750 Met Lys Gln Thr Phe Leu Thr Val Met Lys Thr Phe Tyr Tyr Lys Ala 755 760 765 T rp cys Asp Pro Asn Thr Ile Asn Asp His Ile Ser Lys Val Phe Glu 770 775 780

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Ile Val Ile 785 <210> 130 <211> 527 <212> PRT <213> Streptomyces clavuligerus <400> 130

Met 1 Pro Asp Ala His 5 Asp Ala Pro Pro Pro Gln Ile Arg Gln 10 Arg 15 Thr Leu Val Asp Glu Ala Thr Gl n Leu Leu Thr Glu Ser Ala Glu Asp Ala 20 25 30 T rp Gly Glu Val Ser Val Ser Glu Tyr Glu Thr Ala Arg Leu Val Ala 35 40 45 His Ala Thr T rp Leu Gly Gly His Ala Thr Arg Val Ala Phe Leu Leu 50 55 60 Glu Arg Gln His Glu Asp Gly Ser T rp Gly Pro Pro Gly Gly Tyr Arg 65 70 75 80 Leu Val Pro Thr Leu Ser Ala Val His Ala Leu Leu Thr cys Leu Ala 85 90 95 Ser Pro Ala Gln Asp His Gly Val Pro His Asp Arg Leu Leu Arg Ala 100 105 110 Val Asp Ala Gly Leu Thr Ala Leu Arg Arg Leu Gly Thr Ser Asp Ser 115 120 125 Pro Pro Asp Thr Ile Ala Val Glu Leu Val Ile Pro Ser Leu Leu Glu 130 135 140 Gly Ile Gln His Leu Leu Asp Pro Ala His Pro His Ser Arg Pro Ala 145 150 155 160 Phe Ser Gln His Arg Gly Ser Leu Val cys Pro Gly Gly Leu Asp Gly 165 170 175 Arg Thr Leu Gly Ala Leu Arg Ser His Ala Ala Ala Gly Thr Pro Val 180 185 190 Pro Gly Lys Val T rp His Ala Ser Glu Thr Leu Gly Leu Ser Thr Glu 195 200 205 Ala Ala Ser His Leu Gln Pro Ala Gln Gly Ile Ile Gly Gly Ser Ala 210 215 220 Ala Ala Thr Ala Thr T rp Leu Thr Arg Val Ala Pro Ser Gln Gln Ser 225 230 235 240

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Asp Ser Ala Arg Arg 245 Tyr Leu Glu Glu Leu Gln 250 His Arg Tyr Ser 255 Gly Pro Val Pro Ser Ile Thr Pro Ile Thr Tyr Phe Glu Arg Ala T rp Leu 260 265 270 Leu Asn Asn Phe Ala Ala Ala Gly Val Pro cys Glu Ala Pro Ala Ala 275 280 285 Leu Leu Asp Ser Leu Glu Ala Ala Leu Thr Pro Gln Gly Ala Pro Ala 290 295 300 Gly Ala Gly Leu Pro Pro Asp Ala Asp Asp Thr Ala Ala Val Leu Leu 305 310 315 320 Ala Leu Ala Thr His Gly Arg Gly Arg Arg Pro Glu Val Leu Met Asp 325 330 335 Tyr Arg Thr Asp Gly Tyr Phe Gln cys Phe Ile Gly Glu Arg Thr Pro 340 345 350 Ser Ile Ser Thr Asn Ala His Val Leu Glu Thr Leu Gly His His Val 355 360 365 Ala Gln His Pro Gln Asp Arg Ala Arg Tyr Gly Ser Ala Met Asp Thr 370 375 380 Ala Ser Ala T rp Leu Leu Ala Ala Gln Lys Gln Asp Gly Ser T rp Leu 385 390 395 400 Asp Lys T rp His Ala Ser Pro Tyr Tyr Ala Thr Val cys cys Thr Gln 405 410 415 Ala Leu Ala Ala His Ala Ser Pro Ala Thr Ala Pro Ala Arg Gln Arg 420 425 430 Ala Val Arg T rp Val Leu Ala Thr Gln Arg Ser Asp Gly Gly T rp Gly 435 440 445 Leu T rp His Ser Thr Val Gl u Glu Thr Ala Tyr Ala Leu Gln Ile Leu 450 455 460 Ala Pro Pro Ser Gly Gly Gly Asn Ile Pro Val Gln Gln Ala Leu Thr 465 470 475 480 Arg Gly Arg Ala Arg Leu cys Gly Ala Leu Pro Leu Thr Pro Leu T rp 485 490 495 His Asp Lys Asp Leu Tyr Thr Pro Val Arg Val Val Arg Ala Ala Arg 500 505 510

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Ala Ala Ala Leu Tyr Thr Thr Arg Asp Leu Leu Leu Pro Pro Leu 515 520 525 <210> 131 <211> 516 <212> PRT <213> Bradyrhizobium japonicum

<400> 131 Leu Ser 10 Glu Leu Arg Arg Leu 15 Leu Met 1 Asn Ala Leu Ser 5 Glu His Ile Ser Glu Met Ser Asp Gly Gly Ser Val Gly Pro Ser Val Tyr Asp Thr 20 25 30 Ala Gln Ala Leu Arg Phe His Gly Asn Val Thr Gly Arg Gln Asp Ala 35 40 45 Tyr Ala T rp Leu Ile Ala Gl n Gln Gln Ala Asp Gly Gly T rp Gly Ser 50 55 60 Ala Asp Phe Pro Leu Phe Arg His Ala Pro Thr T rp Ala Ala Leu Leu 65 70 75 80 Ala Leu Gln Arg Ala Asp Pro Leu Pro Gly Ala Ala Asp Ala Val Gln 85 90 95 Thr Ala Thr Arg Phe Leu Gl n Arg Gln Pro Asp Pro Tyr Ala His Ala 100 105 110 Val Pro Glu Asp Ala Pro Ile Gly Ala Glu Leu Ile Leu Pro Gln Phe 115 120 125 cys Gly Glu Ala Ala T rp Leu Leu Gly Gly Val Ala Phe Pro Arg His 130 135 140 Pro Ala Leu Leu Pro Leu Arg Gln Ala cys Leu Val Lys Leu Gly Ala 145 150 155 160 Val Ala Met Leu Pro Ser Gly His Pro Leu Leu His Ser T rp Glu Ala 165 170 175 T rp Gly Thr Ser Pro Thr Thr Ala cys Pro Asp Asp Asp Gly Ser Ile 180 185 190 Gly Ile Ser Pro Ala Ala Thr Ala Ala T rp Arg Ala Gln Ala Val Thr 195 200 205 Arg Gly Ser Thr Pro Gln Val Gly Arg Ala Asp Ala Tyr Leu Gln Met 210 215 220 Ala Ser Arg Ala Thr Arg Ser Gly Ile Glu Gly Val Phe Pro Asn Val 225 230 235 240

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Trp Pro Ile Asn Val Phe Glu Pro cys T rp 250 Ser Leu Tyr Thr Leu 255 His 245 Leu Ala Gly Leu Phe Ala His Pro Ala Leu Ala Glu Ala Val Arg Val 260 265 270 Ile Val Ala Gln Leu Glu Ala Arg Leu Gly Val His Gly Leu Gly Pro 275 280 285 Ala Leu His Phe Ala Ala Asp Ala Asp Asp Thr Ala Val Ala Leu cys 290 295 300 Val Leu His Leu Ala Gly Arg Asp Pro Ala Val Asp Ala Leu Arg His 305 310 315 320 Phe Glu Ile Gly Glu Leu Phe Val Thr Phe Pro Gly Glu Arg Asn Ala 325 330 335 Ser Val Ser Thr Asn Ile His Ala Leu His Ala Leu Arg Leu Leu Gly 340 345 350 Lys Pro Ala Ala Gly Ala Ser Ala Tyr Val Glu Ala Asn Arg Asn Pro 355 360 365 His Gly Leu T rp Asp Asn Gl u Lys T rp His Val Ser T rp Leu Tyr Pro 370 375 380 Thr Ala His Ala Val Ala Ala Leu Ala Gln Gly Lys Pro Gln T rp Arg 385 390 395 400 Asp Glu Arg Ala Leu Ala Ala Leu Leu Gln Ala Gln Arg Asp Asp Gly 405 410 415 Gly T rp Gly Ala Gly Arg Gly Ser Thr Phe Glu Glu Thr Ala Tyr Ala 420 425 430 Leu Phe Ala Leu His Val Met Asp Gly Ser Glu Glu Ala Thr Gly Arg 435 440 445 Arg Arg Ile Ala Gln Val Val Ala Arg Ala Leu Glu T rp Met Leu Ala 450 455 460 Arg His Ala Ala His Gly Leu Pro Gln Thr Pro Leu T rp Ile Gly Lys 465 470 475 480 Glu Leu Tyr cys Pro Thr Arg Val Val Arg Val Ala Glu Leu Ala Gly 485 490 495 Leu T rp Leu Ala Leu Arg Trp Gly Arg Arg Val Leu Ala Glu Gly Ala 500 505 510

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Gly Ala Ala Pro 515 <210> 132 <211> 784 <212> PRT <213> Stevia rebaudiana <400> 132

Met 1 Asn Leu Ser Leu 5 Cys Ile Ala Ser Pro 10 Leu Leu Thr Lys Ser 15 Asn Arg Pro Ala Ala Leu Ser Ala Ile His Thr Ala Ser Thr Ser His Gly 20 25 30 Gly Gln Thr Asn Pro Thr Asn Leu Ile Ile Asp Thr Thr Lys Glu Arg 35 40 45 Ile Gln Lys Gln Phe Lys Asn Val Glu Ile Ser Val Ser Ser Tyr Asp 50 55 60 Thr Ala T rp Val Ala Met Val Pro Ser Pro Asn Ser Pro Lys Ser Pro 65 70 75 80 Cys Phe Pro Glu Cys Leu Asn T rp Leu Ile Asn Asn Gln Leu Asn Asp 85 90 95 Gly Ser T rp Gly Leu Val Asn His Thr His Asn His Asn His Pro Leu 100 105 110 Leu Lys Asp Ser Leu Ser Ser Thr Leu Ala Cys Ile Val Ala Leu Lys 115 120 125 Arg T rp Asn Val Gly Glu Asp Gln Ile Asn Lys Gly Leu Ser Phe Ile 130 135 140 Glu Ser Asn Leu Ala Ser Ala Thr Glu Lys Ser Gln Pro Ser Pro Ile 145 150 155 160 Gly Phe Asp Ile Ile Phe Pro Gly Leu Leu Glu Tyr Ala Lys Asn Leu 165 170 175 Asp Ile Asn Leu Leu Ser Lys Gln Thr Asp Phe Ser Leu Met Leu His 180 185 190 Lys Arg Glu Leu Glu Gln Lys Arg Cys His Ser Asn Glu Met Asp Gly 195 200 205 Tyr Leu Ala Tyr Ile Ser Gl u Gly Leu Gly Asn Leu Tyr Asp T rp Asn 210 215 220 Met Val Lys Lys Tyr Gln Met Lys Asn Gly Ser Val Phe Asn Ser Pro 225 230 235 240

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Ser Ala Thr Ala Ala Ala Phe Ile Asn His 250 Gln Asn Pro Gly cys 255 Leu 245 Asn Tyr Leu Asn Ser Leu Leu Asp Lys Phe Gly Asn Ala Val Pro Thr 260 265 270 Val Tyr Pro His Asp Leu Phe Ile Arg Leu Ser Met Val Asp Thr Ile 275 280 285 Glu Arg Leu Gly Ile Ser His His Phe Arg Val Glu Ile Lys Asn Val 290 295 300 Leu Asp Glu Thr Tyr Arg cys T rp Val Glu Arg Asp Glu Gln Ile Phe 305 310 315 320 Met Asp Val Val Thr cys Ala Leu Ala Phe Arg Leu Leu Arg Ile Asn 325 330 335 Gly Tyr Glu Val Ser Pro Asp Pro Leu Ala Glu Ile Thr Asn Glu Leu 340 345 350 Ala Leu Lys Asp Glu Tyr Ala Ala Leu Glu Thr Tyr His Ala Ser His 355 360 365 Ile Leu Tyr Gln Glu Asp Leu Ser Ser Gly Lys Gln Ile Leu Lys Ser 370 375 380 Ala Asp Phe Leu Lys Glu Ile Ile Ser Thr Asp Ser Asn Arg Leu Ser 385 390 395 400 Lys Leu Ile His Lys Glu Val Glu Asn Ala Leu Lys Phe Pro Ile Asn 405 410 415 Thr Gly Leu Glu Arg Ile Asn Thr Arg Arg Asn Ile Gln Leu Tyr Asn 420 425 430 Val Asp Asn Thr Arg Ile Leu Lys Thr Thr Tyr His Ser Ser Asn Ile 435 440 445 Ser Asn Thr Asp Tyr Leu Arg Leu Ala Val Glu Asp Phe Tyr Thr cys 450 455 460 Gln Ser Ile Tyr Arg Glu Gl u Leu Lys Gly Leu Glu Arg T rp Val Val 465 470 475 480 Glu Asn Lys Leu Asp Gln Leu Lys Phe Ala Arg Gln Lys Thr Ala Tyr 485 490 495 cys Tyr Phe Ser Val Ala Ala Thr Leu Ser Ser Pro Glu Leu Ser Asp 500 505 510

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Ala Arg Ile Ser Trp Ala Lys Asn Gly Ile Leu Thr Thr 525 Val Val Asp 515 520 Asp Phe Phe Asp Ile Gly Gly Thr Ile Asp Glu Leu Thr Asn Leu Ile 530 535 540 Gln cys Val Glu Lys T rp Asn Val Asp Val Asp Lys Asp cys cys Ser 545 550 555 560 Glu His Val Arg Ile Leu Phe Leu Ala Leu Lys Asp Ala Ile cys T rp 565 570 575 Ile Gly Asp Glu Ala Phe Lys T rp Gln Ala Arg Asp Val Thr Ser His 580 585 590 Val Ile Gln Thr T rp Leu Gl u Leu Met Asn Ser Met Leu Arg Glu Ala 595 600 605 Ile T rp Thr Arg Asp Ala Tyr Val Pro Thr Leu Asn Glu Tyr Met Glu 610 615 620 Asn Ala Tyr Val Ser Phe Ala Leu Gly Pro Ile Val Lys Pro Ala Ile 625 630 635 640 Tyr Phe Val Gly Pro Lys Leu Ser Glu Glu Ile Val Glu Ser Ser Glu 645 650 655 Tyr His Asn Leu Phe Lys Leu Met Ser Thr Gln Gly Arg Leu Leu Asn 660 665 670 Asp Ile His Ser Phe Lys Arg Glu Phe Lys Glu Gly Lys Leu Asn Ala 675 680 685 Val Ala Leu His Leu Ser Asn Gly Glu Ser Gly Lys Val Glu Glu Glu 690 695 700 Val Val Glu Glu Met Met Met Met Ile Lys Asn Lys Arg Lys Glu Leu 705 710 715 720 Met Lys Leu Ile Phe Glu Gl u Asn Gly Ser Ile Val Pro Arg Ala cys 725 730 735 Lys Asp Ala Phe T rp Asn Met cys His Val Leu Asn Phe Phe Tyr Ala 740 745 750 Asn Asp Asp Gly Phe Thr Gly Asn Thr Ile Leu Asp Thr Val Lys Asp 755 760 765 Ile Ile Tyr Asn Pro Leu Val Leu Val Asn Glu Asn Glu Glu Gln Arg 770 775 780

<210> 133

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2018200459 19 Jan 2018 <211> 784 <212> PRT <213> Stevia rebaudiana <400> 133

Met 1 Asn Leu Ser Leu 5 cys Ile Ala Ser Pro 10 Leu Leu Thr Lys Ser 15 Ser Arg Pro Thr Ala Leu Ser Ala Ile His Thr Ala Ser Thr Ser His Gly 20 25 30 Gly Gln Thr Asn Pro Thr Asn Leu Ile Ile Asp Thr Thr Lys Glu Arg 35 40 45 Ile Gln Lys Leu Phe Lys Asn Val Glu Ile Ser Val Ser Ser Tyr Asp 50 55 60 Thr Ala T rp Val Ala Met Val Pro Ser Pro Asn Ser Pro Lys Ser Pro 65 70 75 80 cys Phe Pro Glu cys Leu Asn T rp Leu Ile Asn Asn Gln Leu Asn Asp 85 90 95 Gly Ser T rp Gly Leu Val Asn His Thr His Asn His Asn His Pro Leu 100 105 110 Leu Lys Asp Ser Leu Ser Ser Thr Leu Ala cys Ile Val Ala Leu Lys 115 120 125 Arg T rp Asn Val Gly Glu Asp Gln Ile Asn Lys Gly Leu Ser Phe Ile 130 135 140 Glu Ser Asn Leu Ala Ser Ala Thr Asp Lys Ser Gln Pro Ser Pro Ile 145 150 155 160 Gly Phe Asp Ile Ile Phe Pro Gly Leu Leu Glu Tyr Ala Lys Asn Leu 165 170 175 Asp Ile Asn Leu Leu Ser Lys Gln Thr Asp Phe Ser Leu Met Leu His 180 185 190 Lys Arg Glu Leu Glu Gln Lys Arg cys His Ser Asn Glu Ile Asp Gly 195 200 205 Tyr Leu Ala Tyr Ile Ser Gl u Gly Leu Gly Asn Leu Tyr Asp T rp Asn 210 215 220 Met Val Lys Lys Tyr Gln Met Lys Asn Gly Ser Val Phe Asn Ser Pro 225 230 235 240 Ser Ala Thr Ala Ala Ala Phe Ile Asn His Gln Asn Pro Gly cys Leu 245 250 255

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Asn Tyr Leu Asn Ser Leu Leu Asp Lys Phe Gly Asn Ala Val Pro Thr 260 265 270 Val Tyr Pro Leu Asp Leu Tyr Ile Arg Leu Ser Met Val Asp Thr Ile 275 280 285 Glu Arg Leu Gly Ile Ser His His Phe Arg Val Glu Ile Lys Asn Val 290 295 300 Leu Asp Glu Thr Tyr Arg cys T rp Val Glu Arg Asp Glu Gln Ile Phe 305 310 315 320 Met Asp Val Val Thr cys Ala Leu Ala Phe Arg Leu Leu Arg Ile His 325 330 335 Gly Tyr Lys Val Ser Pro Asp Gln Leu Ala Glu Ile Thr Asn Glu Leu 340 345 350 Ala Phe Lys Asp Glu Tyr Ala Ala Leu Glu Thr Tyr His Ala Ser Gln 355 360 365 Ile Leu Tyr Gln Glu Asp Leu Ser Ser Gly Lys Gln Ile Leu Lys Ser 370 375 380 Ala Asp Phe Leu Lys Gly Ile Leu Ser Thr Asp Ser Asn Arg Leu Ser 385 390 395 400 Lys Leu Ile His Lys Glu Val Glu Asn Ala Leu Lys Phe Pro Ile Asn 405 410 415 Thr Gly Leu Glu Arg Ile Asn Thr Arg Arg Asn Ile Gln Leu Tyr Asn 420 425 430 Val Asp Asn Thr Arg Ile Leu Lys Thr Thr Tyr His Ser Ser Asn Ile 435 440 445 Ser Asn Thr Tyr Tyr Leu Arg Leu Ala Val Glu Asp Phe Tyr Thr cys 450 455 460 Gln Ser Ile Tyr Arg Glu Gl u Leu Lys Gly Leu Glu Arg T rp Val Val 465 470 475 480 Gln Asn Lys Leu Asp Gln Leu Lys Phe Ala Arg Gln Lys Thr Ala Tyr 485 490 495 cys Tyr Phe Ser Val Ala Ala Thr Leu Ser Ser Pro Glu Leu Ser Asp 500 505 510 Ala Arg Ile Ser T rp Ala Lys Asn Gly Ile Leu Thr Thr Val Val Asp 515 520 525 Asp Phe Phe Asp Ile Gly Gly Thr Ile Asp Glu Leu Thr Asn Leu Ile

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530 535 540 Gln cys Val Glu Lys T rp Asn Val Asp Val Asp Lys Asp cys cys Ser 545 550 555 560 Glu His Val Arg Ile Leu Phe Leu Ala Leu Lys Asp Ala Ile cys T rp 565 570 575 Ile Gly Asp Glu Ala Phe Lys T rp Gln Ala Arg Asp Val Thr Ser His 580 585 590 Val Ile Gln Thr T rp Leu Gl u Leu Met Asn Ser Met Leu Arg Glu Ala 595 600 605 Ile T rp Thr Arg Asp Ala Tyr Val Pro Thr Leu Asn Glu Tyr Met Glu 610 615 620 Asn Ala Tyr Val Ser Phe Ala Leu Gly Pro Ile Val Lys Pro Ala Ile 625 630 635 640 Tyr Phe Val Gly Pro Lys Leu Ser Glu Glu Ile Val Glu Ser Ser Glu 645 650 655 Tyr His Asn Leu Phe Lys Leu Met Ser Thr Gln Gly Arg Leu Leu Asn 660 665 670 Asp Ile His Ser Phe Lys Arg Glu Phe Lys Glu Gly Lys Leu Asn Ala 675 680 685 Val Ala Leu His Leu Ser Asn Gly Glu Ser Gly Lys Val Glu Glu Glu 690 695 700 Val Val Glu Glu Met Met Met Met Ile Lys Asn Lys Arg Lys Glu Leu 705 710 715 720 Met Lys Leu Ile Phe Glu Gl u Asn Gly Ser Ile Val Pro Arg Ala cys 725 730 735 Lys Asp Ala Phe T rp Asn Met cys His Val Leu Asn Phe Phe Tyr Ala 740 745 750 Asn Asp Asp Gly Phe Thr Gly Asn Thr Ile Leu Asp Thr Val Lys Asp 755 760 765 Ile Ile Tyr Asn Pro Leu Val Leu Val Asn Glu Asn Glu Glu Gln Arg 770 775 780

<210> 134 <211> 590 <212> PRT <213> Zea mays <400> 134

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Met Ala Met Pro Val Lys Leu Thr Pro Ala Ser Leu Ser Leu Lys Ala 1 5 10 15 Val cys cys Arg Phe Ser Ser Gly Gly His Ala Leu Arg Phe Gly Ser 20 25 30 Ser Leu Pro cys T rp Arg Arg Thr Pro Thr Gln Arg Ser Thr Ser Ser 35 40 45 Ser Thr Thr Arg Pro Ala Ala Glu Val Ser Ser Gly Lys Ser Lys Gln 50 55 60 His Asp Gln Glu Ala Ser Gl u Ala Thr Ile Arg Gln Gln Leu Gln Leu 65 70 75 80 Val Asp Val Leu Glu Asn Met Gly Ile Ser Arg His Phe Ala Ala Glu 85 90 95 Ile Lys cys Ile Leu Asp Arg Thr Tyr Arg Ser T rp Leu Gln Arg His 100 105 110 Glu Glu Ile Met Leu Asp Thr Met Thr cys Ala Met Ala Phe Arg Ile 115 120 125 Leu Arg Leu Asn Gly Tyr Asn Val Ser Ser Asp Glu Leu Tyr His Val 130 135 140 Val Glu Ala Ser Gly Leu His Asn Ser Leu Gly Gly Tyr Leu Asn Asp 145 150 155 160 Thr Arg Thr Leu Leu Glu Leu His Lys Ala Ser Thr Val Ser Ile Ser 165 170 175 Glu Asp Glu Ser Ile Leu Asp Ser Ile Gly Ser Arg Ser Arg Thr Leu 180 185 190 Leu Arg Glu Gln Leu Glu Ser Gly Gly Ala Leu Arg Lys Pro Ser Leu 195 200 205 Phe Lys Glu Val Glu His Ala Leu Asp Gly Pro Phe Tyr Thr Thr Leu 210 215 220 Asp Arg Leu His His Arg Trp Asn Ile Glu Asn Phe Asn Ile Ile Glu 225 230 235 240 Gln His Met Leu Glu Thr Pro Tyr Leu Ser Asn Gln His Thr Ser Arg 245 250 255 Asp Ile Leu Ala Leu Ser Ile Arg Asp Phe Ser Ser Ser Gln Phe Thr 260 265 270 Tyr Gln Gln Glu Leu Gln His Leu Glu Ser T rp Val Lys Glu cys Arg

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275 280 285 Leu Asp Gln Leu Gln Phe Ala Arg Gln Lys Leu Ala Tyr Phe Tyr Leu 290 295 300 Ser Ala Ala Gly Thr Met Phe Ser Pro Glu Leu Ser Asp Ala Arg Thr 305 310 315 320 Leu T rp Ala Lys Asn Gly Val Leu Thr Thr Ile Val Asp Asp Phe Phe 325 330 335 Asp Val Ala Gly Ser Lys Gl u Glu Leu Glu Asn Leu Val Met Leu Val 340 345 350 Glu Met T rp Asp Glu His His Lys Val Glu Phe Tyr Ser Glu Gln Val 355 360 365 Glu Ile Ile Phe Ser Ser Ile Tyr Asp Ser Val Asn Gln Leu Gly Glu 370 375 380 Lys Ala Ser Leu Val Gln Asp Arg Ser Ile Thr Lys His Leu Val Glu 385 390 395 400 Ile T rp Leu Asp Leu Leu Lys Ser Met Met Thr Glu Val Glu T rp Arg 405 410 415 Leu Ser Lys Tyr Val Pro Thr Glu Lys Glu Tyr Met Ile Asn Ala Ser 420 425 430 Leu Ile Phe Gly Leu Gly Pro Ile Val Leu Pro Ala Leu Tyr Phe Val 435 440 445 Gly Pro Lys Ile Ser Glu Ser Ile Val Lys Asp Pro Glu Tyr Asp Glu 450 455 460 Leu Phe Lys Leu Met Ser Thr cys Gly Arg Leu Leu Asn Asp Val Gln 465 470 475 480 Thr Phe Glu Arg Glu Tyr Asn Glu Gly Lys Leu Asn Ser Val Ser Leu 485 490 495 Leu Val Leu His Gly Gly Pro Met Ser Ile Ser Asp Ala Lys Arg Lys 500 505 510 Leu Gln Lys Pro Ile Asp Thr cys Arg Arg Asp Leu Leu Ser Leu Val 515 520 525 Leu Arg Glu Glu Ser Val Val Pro Arg Pro cys Lys Glu Leu Phe T rp 530 535 540 Lys Met cys Lys Val cys Tyr Phe Phe Tyr Ser Thr Thr Asp Gly Phe 545 550 555 560

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Ser Ser Gln Val Glu Arg Ala Lys Glu Val Asp Ala Val Ile Asn Glu 565 570 575 Pro Leu Lys Leu Gln Gly Ser His Thr Leu Val Ser Asp Val

580 585 590 <210> 135 <211> 743 <212> PRT <213> Populus trichocarpa

<400> 135 His 5 Gly Thr Lys Glu Arg 10 Ile Lys Lys Met Phe 15 Asp Met 1 Gln Asn Phe Lys Ile Glu Leu Ser Val Ser Ser Tyr Asp Thr Ala T rp Val Ala Met 20 25 30 Val Pro Ser Pro Asp cys Pro Glu Thr Pro cys Phe Pro Glu cys Thr 35 40 45 Lys T rp Ile Leu Glu Asn Gl n Leu Gly Asp Gly Ser T rp Ser Leu Pro 50 55 60 His Gly Asn Pro Leu Leu Val Lys Asp Ala Leu Ser Ser Thr Leu Ala 65 70 75 80 cys Ile Leu Ala Leu Lys Arg T rp Gly Ile Gly Glu Glu Gln Ile Asn 85 90 95 Lys Gly Leu Arg Phe Ile Gl u Leu Asn Ser Ala Ser Val Thr Asp Asn 100 105 110 Glu Gln His Lys Pro Ile Gly Phe Asp Ile Ile Phe Pro Gly Met Ile 115 120 125 Glu Tyr Ala Ile Asp Leu Asp Leu Asn Leu Pro Leu Lys Pro Thr Asp 130 135 140 Ile Asn Ser Met Leu His Arg Arg Ala Leu Glu Leu Thr Ser Gly Gly 145 150 155 160 Gly Lys Asn Leu Glu Gly Arg Arg Ala Tyr Leu Ala Tyr Val Ser Glu 165 170 175 Gly Ile Gly Lys Leu Gln Asp T rp Glu Met Ala Met Lys Tyr Gln Arg 180 185 190 Lys Asn Gly Ser Leu Phe Asn Ser Pro Ser Thr Thr Ala Ala Ala Phe 195 200 205 Ile His Ile Gln Asp Ala Gl u cys Leu His Tyr Ile Arg Ser Leu Leu

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210 215 220

Gln 225 Lys Phe Gly Asn Ala 230 Val Pro Thr Ile Tyr 235 Pro Leu Asp Ile Tyr 240 Ala Arg Leu Ser Met Val Asp Ala Leu Glu Arg Leu Gly Ile Asp Arg 245 250 255 His Phe Arg Lys Glu Arg Lys Phe Val Leu Asp Glu Thr Tyr Arg Phe 260 265 270 T rp Leu Gln Gly Glu Glu Gl u Ile Phe Ser Asp Asn Ala Thr cys Ala 275 280 285 Leu Ala Phe Arg Ile Leu Arg Leu Asn Gly Tyr Asp Val Ser Leu Glu 290 295 300 Asp His Phe Ser Asn Ser Leu Gly Gly Tyr Leu Lys Asp Ser Gly Ala 305 310 315 320 Ala Leu Glu Leu Tyr Arg Ala Leu Gln Leu Ser Tyr Pro Asp Glu Ser 325 330 335 Leu Leu Glu Lys Gln Asn Ser Arg Thr Ser Tyr Phe Leu Lys Gln Gly 340 345 350 Leu Ser Asn Val Ser Leu cys Gly Asp Arg Leu Arg Lys Asn Ile Ile 355 360 365 Gly Glu Val His Asp Ala Leu Asn Phe Ser Asp His Ala Asn Leu Gln 370 375 380 Arg Leu Ala Ile Arg Arg Arg Ile Lys His Tyr Ala Thr Asp Asp Thr 385 390 395 400 Arg Ile Leu Lys Thr Ser Tyr Arg cys Ser Thr Ile Gly Asn Gln Asp 405 410 415 Phe Leu Lys Leu Ala Val Gl u Asp Phe Asn Ile cys Gln Ser Ile Gln 420 425 430 Arg Glu Glu Phe Lys His Ile Glu Arg T rp Val Val Glu Arg Arg Leu 435 440 445 Asp Lys Leu Lys Phe Ala Arg Gln Lys Glu Ala Tyr cys Tyr Phe Ser 450 455 460 Ala Ala Ala Thr Leu Phe Ala Pro Glu Leu Ser Asp Ala Arg Met Ser 465 470 475 480 T rp Ala Lys Asn Gly Val Leu Thr Thr Val Val Asp Asp Phe Phe Asp 485 490 495

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Val Gly Gly Ser 500 Glu Glu Glu Leu Val 505 Asn Leu Ile Glu Leu Ile Glu 510 Arg T rp Asp Val Asn Gly Ser Ala Asp Phe cys Ser Glu Glu Val Glu 515 520 525 Ile Ile Tyr Ser Ala Ile His Ser Thr Ile Ser Glu Ile Gly Asp Lys 530 535 540 Ser Phe Gly T rp Gln Gly Arg Asp Val Lys Ser Gln Val Ile Lys Ile 545 550 555 560 T rp Leu Asp Leu Leu Lys Ser Met Leu Thr Glu Ala Gln T rp Ser Ser 565 570 575 Asn Lys Ser Val Pro Thr Leu Asp Glu Tyr Met Thr Thr Ala His Val 580 585 590 Ser Phe Ala Leu Gly Pro Ile Val Leu Pro Ala Leu Tyr Phe Val Gly 595 600 605 Pro Lys Leu Ser Glu Glu Val Ala Gly His Pro Glu Leu Leu Asn Leu 610 615 620 Tyr Lys Val Thr Ser Thr cys Gly Arg Leu Leu Asn Asp T rp Arg Ser 625 630 635 640 Phe Lys Arg Glu Ser Glu Gl u Gly Lys Leu Asn Ala Ile Ser Leu Tyr 645 650 655 Met Ile His Ser Gly Gly Ala Ser Thr Glu Glu Glu Thr Ile Glu His 660 665 670 Phe Lys Gly Leu Ile Asp Ser Gln Arg Arg Gln Leu Leu Gln Leu Val 675 680 685 Leu Gln Glu Lys Asp Ser Ile Ile Pro Arg Pro cys Lys Asp Leu Phe 690 695 700 T rp Asn Met Ile Lys Leu Leu His Thr Phe Tyr Met Lys Asp Asp Gly 705 710 715 720 Phe Thr Ser Asn Glu Met Arg Asn Val Val Lys Ala Ile Ile Asn Glu 725 730 735 Pro Ile Ser Leu Asp Glu Leu

<210> 136 <211> 983 <212> PRT

740

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2018200459 19 Jan 2018 <213> Phomopsis amygdali <400> 136

Met Glu 1 Phe Asp Glu 5 Pro Leu Val Asp Glu 10 Ala Arg Ser Leu Val 15 Gln Arg Thr Leu Gln Asp Tyr Asp Asp Arg Tyr Gly Phe Gly Thr Met Ser 20 25 30 cys Ala Ala Tyr Asp Thr Ala T rp Val Ser Leu Val Thr Lys Thr Val 35 40 45 Asp Gly Arg Lys Gln T rp Leu Phe Pro Glu cys Phe Glu Phe Leu Leu 50 55 60 Glu Thr Gln Ser Asp Ala Gly Gly T rp Glu Ile Gly Asn Ser Ala Pro 65 70 75 80 Ile Asp Gly Ile Leu Asn Thr Ala Ala Ser Leu Leu Ala Leu Lys Arg 85 90 95 His Val Gln Thr Glu Gln Ile Ile Gln Pro Gln His Asp His Lys Asp 100 105 110 Leu Ala Gly Arg Ala Glu Arg Ala Ala Ala Ser Leu Arg Ala Gln Leu 115 120 125 Ala Ala Leu Asp Val Ser Thr Thr Glu His Val Gly Phe Glu Ile Ile 130 135 140 Val Pro Ala Met Leu Asp Pro Leu Glu Ala Glu Asp Pro Ser Leu Val 145 150 155 160 Phe Asp Phe Pro Ala Arg Lys Pro Leu Met Lys Ile His Asp Ala Lys 165 170 175 Met Ser Arg Phe Arg Pro Gl u Tyr Leu Tyr Gly Lys Gln Pro Met Thr 180 185 190 Ala Leu His Ser Leu Glu Ala Phe Ile Gly Lys Ile Asp Phe Asp Lys 195 200 205 Val Arg His His Arg Thr His Gly Ser Met Met Gly Ser Pro Ser Ser 210 215 220 Thr Ala Ala Tyr Leu Met His Ala Ser Gln T rp Asp Gly Asp Ser Glu 225 230 235 240 Ala Tyr Leu Arg His Val Ile Lys His Ala Ala Gly Gln Gly Thr Gly 245 250 255 Ala Val Pro Ser Ala Phe Pro Ser Thr His Phe Glu Ser Ser T rp Ile 260 265 270

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Leu Thr Thr Leu Phe Arg Ala Gly Phe Ser Ala Ser His Leu Ala cys 275 280 285 Asp Glu Leu Asn Lys Leu Val Glu Ile Leu Glu Gly Ser Phe Glu Lys 290 295 300 Glu Gly Gly Ala Ile Gly Tyr Ala Pro Gly Phe Gln Ala Asp Val Asp 305 310 315 320 Asp Thr Ala Lys Thr Ile Ser Thr Leu Ala Val Leu Gly Arg Asp Ala 325 330 335 Thr Pro Arg Gln Met Ile Lys Val Phe Glu Ala Asn Thr His Phe Arg 340 345 350 Thr Tyr Pro Gly Glu Arg Asp Pro Ser Leu Thr Ala Asn cys Asn Ala 355 360 365 Leu Ser Ala Leu Leu His Gl n Pro Asp Ala Ala Met Tyr Gly Ser Gln 370 375 380 Ile Gln Lys Ile Thr Lys Phe Val cys Asp Tyr T rp T rp Lys Ser Asp 385 390 395 400 Gly Lys Ile Lys Asp Lys Trp Asn Thr cys Tyr Leu Tyr Pro Ser Val 405 410 415 Leu Leu Val Glu Val Leu Val Asp Leu Val Ser Leu Leu Glu Gln Gly 420 425 430 Lys Leu Pro Asp Val Leu Asp Gln Glu Leu Gln Tyr Arg Val Ala Ile 435 440 445 Thr Leu Phe Gln Ala cys Leu Arg Pro Leu Leu Asp Gln Asp Ala Glu 450 455 460 Gly Ser T rp Asn Lys Ser Ile Glu Ala Thr Ala Tyr Gly Ile Leu Ile 465 470 475 480 Leu Thr Glu Ala Arg Arg Val cys Phe Phe Asp Arg Leu Ser Glu Pro 485 490 495 Leu Asn Glu Ala Ile Arg Arg Gly Ile Ala Phe Ala Asp Ser Met Ser 500 505 510 Gly Thr Glu Ala Gln Leu Asn Tyr Ile T rp Ile Glu Lys Val Ser Tyr 515 520 525 Ala Pro Ala Leu Leu Thr Lys Ser Tyr Leu Leu Ala Ala Arg T rp Ala

530 535 540

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Ala 545 Lys Ser Pro Leu Gly Ala 550 Ser Val Gly Ser Ser 555 Leu T rp Thr Pro 560 Pro Arg Glu Gly Leu Asp Lys His Val Arg Leu Phe His Gln Ala Glu 565 570 575 Leu Phe Arg Ser Leu Pro Gl u T rp Glu Leu Arg Ala Ser Met Ile Glu 580 585 590 Ala Ala Leu Phe Thr Pro Leu Leu Arg Ala His Arg Leu Asp Val Phe 595 600 605 Pro Arg Gln Asp Val Gly Gl u Asp Lys Tyr Leu Asp Val Val Pro Phe 610 615 620 Phe T rp Thr Ala Ala Asn Asn Arg Asp Arg Thr Tyr Ala Ser Thr Leu 625 630 635 640 Phe Leu Tyr Asp Met cys Phe Ile Ala Met Leu Asn Phe Gln Leu Asp 645 650 655 Glu Phe Met Glu Ala Thr Ala Gly Ile Leu Phe Arg Asp His Met Asp 660 665 670 Asp Leu Arg Gln Leu Ile His Asp Leu Leu Ala Glu Lys Thr Ser Pro 675 680 685 Lys Ser Ser Gly Arg Ser Ser Gln Gly Thr Lys Asp Ala Asp Ser Gly 690 695 700 Ile Glu Glu Asp Val Ser Met Ser Asp Ser Ala Ser Asp Ser Gln Asp 705 710 715 720 Arg Ser Pro Glu Tyr Asp Leu Val Phe Ser Ala Leu Ser Thr Phe Thr 725 730 735 Lys His Val Leu Gln His Pro Ser Ile Gln Ser Ala Ser Val T rp Asp 740 745 750 Arg Lys Leu Leu Ala Arg Gl u Met Lys Ala Tyr Leu Leu Ala His Ile 755 760 765 Gln Gln Ala Glu Asp Ser Thr Pro Leu Ser Glu Leu Lys Asp Val Pro 770 775 780 Gln Lys Thr Asp Val Thr Arg Val Ser Thr Ser Thr Thr Thr Phe Phe 785 790 795 800 Asn T rp Val Arg Thr Thr Ser Ala Asp His Ile Ser cys Pro Tyr Ser 805 810 815

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Phe His Phe Val 820 Ala cys His Leu Gly Ala Ala Leu Ser Pro Lys Gly 825 830 Ser Asn Gly Asp cys Tyr Pro Ser Ala Gly Glu Lys Phe Leu Ala Ala 835 840 845 Ala Val cys Arg His Leu Ala Thr Met cys Arg Met Tyr Asn Asp Leu 850 855 860 Gly Ser Ala Glu Arg Asp Ser Asp Glu Gly Asn Leu Asn Ser Leu Asp 865 870 875 880 Phe Pro Glu Phe Ala Asp Ser Ala Gly Asn Gly Gly Ile Glu Ile Gln 885 890 895 Lys Ala Ala Leu Leu Arg Leu Ala Glu Phe Glu Arg Asp Ser Tyr Leu 900 905 910 Glu Ala Phe Arg Arg Leu Gl n Asp Glu Ser Asn Arg Val His Gly Pro 915 920 925 Ala Gly Gly Asp Glu Ala Arg Leu Ser Arg Arg Arg Met Ala Ile Leu 930 935 940 Glu Phe Phe Ala Gln Gln Val Asp Leu Tyr Gly Gln Val Tyr Val Ile 945 950 955 960 Arg Asp Ile Ser Ala Arg Ile Pro Lys Asn Glu Val Glu Lys Lys Arg 965 970 975 Lys Leu Asp Asp Ala Phe Asn

980 <210> 137 <211> 881 <212> PRT <213> Physcomitrella patens <400> 137

Met 1 Ala Ser Ser Thr 5 Leu Ile Gln Asn Arg 10 Ser cys Gly Val Thr 15 Ser Ser Met Ser Ser Phe Gln Ile Phe Arg Gly Gln Pro Leu Arg Phe Pro 20 25 30 Gly Thr Arg Thr Pro Ala Ala Val Gln cys Leu Lys Lys Arg Arg cys 35 40 45 Leu Arg Pro Thr Glu Ser Val Leu Glu Ser Ser Pro Gly Ser Gly Ser 50 55 60 Tyr Arg Ile Val Thr Gly Pro Ser Gly Ile Asn Pro Ser Ser Asn Gly 65 70 75 80

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His Leu Gln Glu Gly Ser 85 Leu Thr Hi s Arg 90 Leu Pro Ile Pro Met 95 Glu Lys Ser Ile Asp Asn Phe Gl n Ser Thr Leu Tyr Val Ser Asp Ile T rp 100 105 110 Ser Glu Thr Leu Gln Arg Thr Glu cys Leu Leu Gln Val Thr Glu Asn 115 120 125 Val Gln Met Asn Glu T rp Ile Glu Glu Ile Arg Met Tyr Phe Arg Asn 130 135 140 Met Thr Leu Gly Glu Ile Ser Met Ser Pro Tyr Asp Thr Ala T rp Val 145 150 155 160 Ala Arg Val Pro Ala Leu Asp Gly Ser His Gly Pro Gln Phe His Arg 165 170 175 Ser Leu Gln T rp Ile Ile Asp Asn Gln Leu Pro Asp Gly Asp T rp Gly 180 185 190 Glu Pro Ser Leu Phe Leu Gly Tyr Asp Arg Val cys Asn Thr Leu Ala 195 200 205 cys Val Ile Ala Leu Lys Thr T rp Gly Val Gly Ala Gln Asn Val Glu 210 215 220 Arg Gly Ile Gln Phe Leu Gl n Ser Asn Ile Tyr Lys Met Glu Glu Asp 225 230 235 240 Asp Ala Asn His Met Pro Ile Gly Phe Glu Ile Val Phe Pro Ala Met 245 250 255 Met Glu Asp Ala Lys Ala Leu Gly Leu Asp Leu Pro Tyr Asp Ala Thr 260 265 270 Ile Leu Gln Gln Ile Ser Ala Glu Arg Glu Lys Lys Met Lys Lys Ile 275 280 285 Pro Met Ala Met Val Tyr Lys Tyr Pro Thr Thr Leu Leu His Ser Leu 290 295 300 Glu Gly Leu His Arg Glu Val Asp T rp Asn Lys Leu Leu Gln Leu Gln 305 310 315 320 Ser Glu Asn Gly Ser Phe Leu Tyr Ser Pro Ala Ser Thr Ala cys Ala 325 330 335 Leu Met Tyr Thr Lys Asp Val Lys cys Phe Asp Tyr Leu Asn Gln Leu 340 345 350

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Leu Ile Lys 355 Phe Asp His Ala cys 360 Pro Asn Val Tyr Pro 365 Val Asp Leu Phe Glu Arg Leu T rp Met Val Asp Arg Leu Gln Arg Leu Gly Ile Ser 370 375 380 Arg Tyr Phe Glu Arg Glu Ile Arg Asp cys Leu Gln Tyr Val Tyr Arg 385 390 395 400 Tyr T rp Lys Asp cys Gly Ile Gly T rp Ala Ser Asn Ser Ser Val Gln 405 410 415 Asp Val Asp Asp Thr Ala Met Ala Phe Arg Leu Leu Arg Thr His Gly 420 425 430 Phe Asp Val Lys Glu Asp cys Phe Arg Gln Phe Phe Lys Asp Gly Glu 435 440 445 Phe Phe cys Phe Ala Gly Gl n Ser Ser Gln Ala Val Thr Gly Met Phe 450 455 460 Asn Leu Ser Arg Ala Ser Gl n Thr Leu Phe Pro Gly Glu Ser Leu Leu 465 470 475 480 Lys Lys Ala Arg Thr Phe Ser Arg Asn Phe Leu Arg Thr Lys His Glu 485 490 495 Asn Asn Glu cys Phe Asp Lys T rp Ile Ile Thr Lys Asp Leu Ala Gly 500 505 510 Glu Val Glu Tyr Asn Leu Thr Phe Pro T rp Tyr Ala Ser Leu Pro Arg 515 520 525 Leu Glu His Arg Thr Tyr Leu Asp Gln Tyr Gly Ile Asp Asp Ile T rp 530 535 540 Ile Gly Lys Ser Leu Tyr Lys Met Pro Ala Val Thr Asn Glu Val Phe 545 550 555 560 Leu Lys Leu Ala Lys Ala Asp Phe Asn Met cys Gln Ala Leu His Lys 565 570 575 Lys Glu Leu Glu Gln Val Ile Lys T rp Asn Ala Ser cys Gln Phe Arg 580 585 590 Asp Leu Glu Phe Ala Arg Gl n Lys Ser Val Glu cys Tyr Phe Ala Gly 595 600 605 Ala Ala Thr Met Phe Glu Pro Glu Met Val Gln Ala Arg Leu Val T rp 610 615 620 Ala Arg cys cys Val Leu Thr Thr Val Leu Asp Asp Tyr Phe Asp His Page 179

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625 630 635 640 Gly Thr Pro Val Glu Glu Leu Arg Val Phe Val Gln Ala Val Arg Thr 645 650 655 T rp Asn Pro Glu Leu Ile Asn Gly Leu Pro Glu Gln Ala Lys Ile Leu 660 665 670 Phe Met Gly Leu Tyr Lys Thr Val Asn Thr Ile Ala Glu Glu Ala Phe 675 680 685 Met Ala Gln Lys Arg Asp Val His His His Leu Lys His Tyr T rp Asp 690 695 700 Lys Leu Ile Thr Ser Ala Leu Lys Glu Ala Glu T rp Ala Glu Ser Gly 705 710 715 720 Tyr Val Pro Thr Phe Asp Gl u Tyr Met Glu Val Ala Glu Ile Ser Val 725 730 735 Ala Leu Glu Pro Ile Val Cys Ser Thr Leu Phe Phe Ala Gly His Arg 740 745 750 Leu Asp Glu Asp Val Leu Asp Ser Tyr Asp Tyr His Leu Val Met His 755 760 765 Leu Val Asn Arg Val Gly Arg Ile Leu Asn Asp Ile Gln Gly Met Lys 770 775 780 Arg Glu Ala Ser Gln Gly Lys Ile Ser Ser Val Gln Ile Tyr Met Glu 785 790 795 800 Glu His Pro Ser Val Pro Ser Glu Ala Met Ala Ile Ala His Leu Gln 805 810 815 Glu Leu Val Asp Asn Ser Met Gln Gln Leu Thr Tyr Glu Val Leu Arg 820 825 830 Phe Thr Ala Val Pro Lys Ser Cys Lys Arg Ile His Leu Asn Met Ala 835 840 845 Lys Ile Met His Ala Phe Tyr Lys Asp Thr Asp Gly Phe Ser Ser Leu 850 855 860 Thr Ala Met Thr Gly Phe Val Lys Lys Val Leu Phe Glu Pro Val Pro 865 870 875 880

Glu <210> 138 <211> 513

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2018200459 19 Jan 2018 <212> PRT <213> Stevia rebaudiana

<400> 138 Met 1 Asp Ala Val Thr 5 Gly Leu Leu Thr Val 10 Pro Ala Thr Ala Ile 15 Thr Ile Gly Gly Thr Ala Val Ala Leu Ala Val Ala Leu Ile Phe T rp Tyr 20 25 30 Leu Lys Ser Tyr Thr Ser Ala Arg Arg Ser Gln Ser Asn His Leu Pro 35 40 45 Arg Val Pro Glu Val Pro Gly Val Pro Leu Leu Gly Asn Leu Leu Gln 50 55 60 Leu Lys Glu Lys Lys Pro Tyr Met Thr Phe Thr Arg T rp Ala Ala Thr 65 70 75 80 Tyr Gly Pro Ile Tyr Ser Ile Lys Thr Gly Ala Thr Ser Met Val Val 85 90 95 Val Ser Ser Asn Glu Ile Ala Lys Glu Ala Leu Val Thr Arg Phe Gln 100 105 110 Ser Ile Ser Thr Arg Asn Leu Ser Lys Ala Leu Lys Val Leu Thr Ala 115 120 125 Asp Lys Thr Met Val Ala Met Ser Asp Tyr Asp Asp Tyr His Lys Thr 130 135 140 Val Lys Arg Hi s Ile Leu Thr Ala Val Leu Gly Pro Asn Ala Gln Lys 145 150 155 160 Lys His Arg Ile His Arg Asp Ile Met Met Asp Asn Ile Ser Thr Gln 165 170 175 Leu His Glu Phe Val Lys Asn Asn Pro Glu Gln Glu Glu Val Asp Leu 180 185 190 Arg Lys Ile Phe Gln Ser Glu Leu Phe Gly Leu Ala Met Arg Gln Ala 195 200 205 Leu Gly Lys Asp Val Glu Ser Leu Tyr Val Glu Asp Leu Lys Ile Thr 210 215 220 Met Asn Arg Asp Glu Ile Phe Gln Val Leu Val Val Asp Pro Met Met 225 230 235 240 Gly Ala Ile Asp Val Asp Trp Arg Asp Phe Phe Pro Tyr Leu Lys T rp 245 250 255 Val Pro Asn Lys Lys Phe Glu Asn Thr Ile Gln Gln Met Tyr Ile Arg Page 181

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260 265 270 Arg Glu Ala Val Met Lys Ser Leu Ile Lys Glu His Lys Lys Arg Ile 275 280 285 Ala Ser Gly Glu Lys Leu Asn Ser Tyr Ile Asp Tyr Leu Leu Ser Glu 290 295 300 Ala Gln Thr Leu Thr Asp Gl n Gln Leu Leu Met Ser Leu T rp Glu Pro 305 310 315 320 Ile Ile Glu Ser Ser Asp Thr Thr Met Val Thr Thr Glu T rp Ala Met 325 330 335 Tyr Glu Leu Ala Lys Asn Pro Lys Leu Gln Asp Arg Leu Tyr Arg Asp 340 345 350 Ile Lys Ser Val cys Gly Ser Glu Lys Ile Thr Glu Glu His Leu Ser 355 360 365 Gln Leu Pro Tyr Ile Thr Ala Ile Phe His Glu Thr Leu Arg Arg His 370 375 380 Ser Pro Val Pro Ile Ile Pro Leu Arg His Val His Glu Asp Thr Val 385 390 395 400 Leu Gly Gly Tyr His Val Pro Ala Gly Thr Glu Leu Ala Val Asn Ile 405 410 415 Tyr Gly cys Asn Met Asp Lys Asn Val T rp Glu Asn Pro Glu Glu T rp 420 425 430 Asn Pro Glu Arg Phe Met Lys Glu Asn Glu Thr Ile Asp Phe Gln Lys 435 440 445 Thr Met Ala Phe Gly Gly Gly Lys Arg Val cys Ala Gly Ser Leu Gln 450 455 460 Ala Leu Leu Thr Ala Ser Ile Gly Ile Gly Arg Met Val Gln Glu Phe 465 470 475 480 Glu T rp Lys Leu Lys Asp Met Thr Gln Glu Glu Val Asn Thr Ile Gly 485 490 495 Leu Thr Thr Gln Met Leu Arg Pro Leu Arg Ala Ile Ile Lys Pro Arg 500 505 510

Ile <210> 139 <211> 509

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2018200459 19 Jan 2018 <212> PRT <213> Arabidopsis thaliana

<400> 139 Phe Ser Met Ile Ser Ile 5 Leu 10 Leu Gly Phe Val Ile 15 Ser Met 1 Ala Phe Ser Phe Ile Phe Ile Phe Phe Phe Lys Lys Leu Leu Ser Phe Ser Arg 20 25 30 Lys Asn Met Ser Glu Val Ser Thr Leu Pro Ser Val Pro Val Val Pro 35 40 45 Gly Phe Pro Val Ile Gly Asn Leu Leu Gln Leu Lys Glu Lys Lys Pro 50 55 60 His Lys Thr Phe Thr Arg Trp Ser Glu Ile Tyr Gly Pro Ile Tyr Ser 65 70 75 80 Ile Lys Met Gly Ser Ser Ser Leu Ile Val Leu Asn Ser Thr Glu Thr 85 90 95 Ala Lys Glu Ala Met Val Thr Arg Phe Ser Ser Ile Ser Thr Arg Lys 100 105 110 Leu Ser Asn Ala Leu Thr Val Leu Thr cys Asp Lys Ser Met Val Ala 115 120 125 Thr Ser Asp Tyr Asp Asp Phe His Lys Leu Val Lys Arg cys Leu Leu 130 135 140 Asn Gly Leu Leu Gly Ala Asn Ala Gln Lys Arg Lys Arg His Tyr Arg 145 150 155 160 Asp Ala Leu Ile Glu Asn Val Ser Ser Lys Leu His Ala His Ala Arg 165 170 175 Asp His Pro Gln Glu Pro Val Asn Phe Arg Ala Ile Phe Glu His Glu 180 185 190 Leu Phe Gly Val Ala Leu Lys Gln Ala Phe Gly Lys Asp Val Glu Ser 195 200 205 Ile Tyr Val Lys Glu Leu Gly Val Thr Leu Ser Lys Asp Glu Ile Phe 210 215 220 Lys Val Leu Val His Asp Met Met Glu Gly Ala Ile Asp Val Asp T rp 225 230 235 240 Arg Asp Phe Phe Pro Tyr Leu Lys T rp Ile Pro Asn Lys Ser Phe Glu 245 250 255 Ala Arg Ile Gln Gln Lys His Lys Arg Arg Leu Ala Val Met Asn Ala Page 183

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260 265 270 Leu Ile Gln Asp Arg Leu Lys Gln Asn Gly Ser Glu Ser Asp Asp Asp 275 280 285 cys Tyr Leu Asn Phe Leu Met Ser Glu Ala Lys Thr Leu Thr Lys Glu 290 295 300 Gln Ile Ala Ile Leu Val Trp Glu Thr Ile Ile Glu Thr Ala Asp Thr 305 310 315 320 Thr Leu Val Thr Thr Glu Trp Ala Ile Tyr Glu Leu Ala Lys His Pro 325 330 335 Ser Val Gln Asp Arg Leu cys Lys Glu Ile Gln Asn Val cys Gly Gly 340 345 350 Glu Lys Phe Lys Glu Glu Gl n Leu Ser Gln Val Pro Tyr Leu Asn Gly 355 360 365 Val Phe His Glu Thr Leu Arg Lys Tyr Ser Pro Ala Pro Leu Val Pro 370 375 380 Ile Arg Tyr Ala His Glu Asp Thr Gln Ile Gly Gly Tyr His Val Pro 385 390 395 400 Ala Gly Ser Glu Ile Ala Ile Asn Ile Tyr Gly cys Asn Met Asp Lys 405 410 415 Lys Arg T rp Glu Arg Pro Gl u Asp T rp T rp Pro Glu Arg Phe Leu Asp 420 425 430 Asp Gly Lys Tyr Glu Thr Ser Asp Leu His Lys Thr Met Ala Phe Gly 435 440 445 Ala Gly Lys Arg Val cys Ala Gly Ala Leu Gln Ala Ser Leu Met Ala 450 455 460 Gly Ile Ala Ile Gly Arg Leu Val Gln Glu Phe Glu T rp Lys Leu Arg 465 470 475 480 Asp Gly Glu Glu Glu Asn Val Asp Thr Tyr Gly Leu Thr Ser Gln Lys 485 490 495 Leu Tyr Pro Leu Met Ala Ile Ile Asn Pro Arg Arg Ser 500 505

<210> 140 <211> 525 <212> PRT <213> Gibberella fujikoroi <400> 140

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Met 1 Ser Lys Ser Asn 5 Ser Met Asn Ser Thr Ser His 10 Glu Thr Leu 15 Phe Gln Gln Leu Val Leu Gly Leu Asp Arg Met Pro Leu Met Asp Val His 20 25 30 T rp Leu Ile Tyr Val Ala Phe Gly Ala T rp Leu cys Ser Tyr Val Ile 35 40 45 His Val Leu Ser Ser Ser Ser Thr Val Lys Val Pro Val Val Gly Tyr 50 55 60 Arg Ser Val Phe Glu Pro Thr T rp Leu Leu Arg Leu Arg Phe Val T rp 65 70 75 80 Glu Gly Gly Ser Ile Ile Gly Gln Gly Tyr Asn Lys Phe Lys Asp Ser 85 90 95 Ile Phe Gln Val Arg Lys Leu Gly Thr Asp Ile Val Ile Ile Pro Pro 100 105 110 Asn Tyr Ile Asp Glu Val Arg Lys Leu Ser Gln Asp Lys Thr Arg Ser 115 120 125 Val Glu Pro Phe Ile Asn Asp Phe Ala Gly Gln Tyr Thr Arg Gly Met 130 135 140 Val Phe Leu Gln Ser Asp Leu Gln Asn Arg Val Ile Gln Gln Arg Leu 145 150 155 160 Thr Pro Lys Leu Val Ser Leu Thr Lys Val Met Lys Glu Glu Leu Asp 165 170 175 Tyr Ala Leu Thr Lys Glu Met Pro Asp Met Lys Asn Asp Glu T rp Val 180 185 190 Glu Val Asp Ile Ser Ser Ile Met Val Arg Leu Ile Ser Arg Ile Ser 195 200 205 Ala Arg Val Phe Leu Gly Pro Glu His cys Arg Asn Gln Glu T rp Leu 210 215 220 Thr Thr Thr Ala Glu Tyr Ser Glu Ser Leu Phe Ile Thr Gly Phe Ile 225 230 235 240 Leu Arg Val Val Pro His Ile Leu Arg Pro Phe Ile Ala Pro Leu Leu 245 250 255 Pro Ser Tyr Arg Thr Leu Leu Arg Asn Val Ser Ser Gly Arg Arg Val 260 265 270 Ile Gly Asp Ile Ile Arg Ser Gln Gln Gly Asp Gly Asn Glu Asp Ile Page 185

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275 280 285 Leu Ser T rp Met Arg Asp Ala Ala Thr Gly Glu Glu Lys Gln Ile Asp 290 295 300 Asn Ile Ala Gln Arg Met Leu Ile Leu Ser Leu Ala Ser Ile His Thr 305 310 315 320 Thr Ala Met Thr Met Thr His Ala Met Tyr Asp Leu cys Ala cys Pro 325 330 335 Glu Tyr Ile Glu Pro Leu Arg Asp Glu Val Lys Ser Val Val Gly Ala 340 345 350 Ser Gly T rp Asp Lys Thr Ala Leu Asn Arg Phe His Lys Leu Asp Ser 355 360 365 Phe Leu Lys Glu Ser Gln Arg Phe Asn Pro Val Phe Leu Leu Thr Phe 370 375 380 Asn Arg Ile Tyr His Gln Ser Met Thr Leu Ser Asp Gly Thr Asn Ile 385 390 395 400 Pro Ser Gly Thr Arg Ile Ala Val Pro Ser His Ala Met Leu Gln Asp 405 410 415 Ser Ala His Val Pro Gly Pro Thr Pro Pro Thr Glu Phe Asp Gly Phe 420 425 430 Arg Tyr Ser Lys Ile Arg Ser Asp Ser Asn Tyr Ala Gln Lys Tyr Leu 435 440 445 Phe Ser Met Thr Asp Ser Ser Asn Met Ala Phe Gly Tyr Gly Lys Tyr 450 455 460 Ala cys Pro Gly Arg Phe Tyr Ala Ser Asn Glu Met Lys Leu Thr Leu 465 470 475 480 Ala Ile Leu Leu Leu Gln Phe Glu Phe Lys Leu Pro Asp Gly Lys Gly 485 490 495 Arg Pro Arg Asn Ile Thr Ile Asp Ser Asp Met Ile Pro Asp Pro Arg 500 505 510 Ala Arg Leu cys Val Arg Lys Arg Ser Leu Arg Asp Glu 515 520 525

<210> 141 <211> 499 <212> PRT <213> Trametes versicolor <400> 141

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Met 1 Glu Asp Pro Thr Val 5 Leu Tyr Ala cys 10 Leu Ala Ile Ala Val 15 Ala Thr Phe Val Val Arg T rp Tyr Arg Asp Pro Leu Arg Ser Ile Pro Thr 20 25 30 Val Gly Gly Ser Asp Leu Pro Ile Leu Ser Tyr Ile Gly Ala Leu Arg 35 40 45 T rp Thr Arg Arg Gly Arg Gl u Ile Leu Gln Glu Gly Tyr Asp Gly Tyr 50 55 60 Arg Gly Ser Thr Phe Lys Ile Ala Met Leu Asp Arg T rp Ile Val Ile 65 70 75 80 Ala Asn Gly Pro Lys Leu Ala Asp Glu Val Arg Arg Arg Pro Asp Glu 85 90 95 Glu Leu Asn Phe Met Asp Gly Leu Gly Ala Phe Val Gln Thr Lys Tyr 100 105 110 Thr Leu Gly Glu Ala Ile His Asn Asp Pro Tyr His Val Asp Ile Ile 115 120 125 Arg Glu Lys Leu Thr Arg Gly Leu Pro Ala Val Leu Pro Asp Val Ile 130 135 140 Glu Glu Leu Thr Leu Ala Val Arg Gln Tyr Ile Pro Thr Glu Gly Asp 145 150 155 160 Glu T rp Val Ser Val Asn cys Ser Lys Ala Ala Arg Asp Ile Val Ala 165 170 175 Arg Ala Ser Asn Arg Val Phe Val Gly Leu Pro Ala cys Arg Asn Gln 180 185 190 Gly Tyr Leu Asp Leu Ala Ile Asp Phe Thr Leu Ser Val Val Lys Asp 195 200 205 Arg Ala Ile Ile Asn Met Phe Pro Glu Leu Leu Lys Pro Ile Val Gly 210 215 220 Arg Val Val Gly Asn Ala Thr Arg Asn Val Arg Arg Ala Val Pro Phe 225 230 235 240 Val Ala Pro Leu Val Glu Gl u Arg Arg Arg Leu Met Glu Glu Tyr Gly 245 250 255 Glu Asp T rp Ser Glu Lys Pro Asn Asp Met Leu Gln T rp Ile Met Asp 260 265 270 Glu Ala Ala Ser Arg Asp Ser Ser Val Lys Ala Ile Ala Glu Arg Leu

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275 280 285 Leu Met Val Asn Phe Ala Ala Ile His Thr Ser Ser Asn Thr Ile Thr 290 295 300 His Ala Leu Tyr His Leu Ala Glu Met Pro Glu Thr Leu Gln Pro Leu 305 310 315 320 Arg Glu Glu Ile Glu Pro Leu Val Lys Glu Glu Gly T rp Thr Lys Ala 325 330 335 Ala Met Gly Lys Met T rp Trp Leu Asp Ser Phe Leu Arg Glu Ser Gln 340 345 350 Arg Tyr Asn Gly Ile Asn Ile Val Ser Leu Thr Arg Met Ala Asp Lys 355 360 365 Asp Ile Thr Leu Ser Asp Gly Thr Phe Leu Pro Lys Gly Thr Leu Val 370 375 380 Ala Val Pro Ala Tyr Ser Thr His Arg Asp Asp Ala Val Tyr Ala Asp 385 390 395 400 Ala Leu Val Phe Asp Pro Phe Arg Phe Ser Arg Met Arg Ala Arg Glu 405 410 415 Gly Glu Gly Thr Lys His Gl n Phe Val Asn Thr Ser Val Glu Tyr Val 420 425 430 Pro Phe Gly His Gly Lys His Ala cys Pro Gly Arg Phe Phe Ala Ala 435 440 445 Asn Glu Leu Lys Ala Met Leu Ala Tyr Ile Val Leu Asn Tyr Asp Val 450 455 460 Lys Leu Pro Gly Asp Gly Lys Arg Pro Leu Asn Met Tyr T rp Gly Pro 465 470 475 480 Thr Val Leu Pro Ala Pro Ala Gly Gln Val Leu Phe Arg Lys Arg Gln 485 490 495

Val Ser Leu <210> 142 <211> 525 <212> PRT <213> Stevia rebaudiana <400> 142

Met Gly Leu Phe Pro Leu Glu Asp Ser Tyr Ala Leu Val Phe Glu Gly 15 10 15

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Leu Ala Ile Thr 20 Leu Ala Leu Tyr Tyr 25 Leu Leu Ser Phe Ile 30 Tyr Lys Thr Ser Lys Lys Thr cys Thr Pro Pro Lys Ala Ser Gly Glu Ile Ile 35 40 45 Pro Ile Thr Gly Ile Ile Leu Asn Leu Leu Ser Gly Ser Ser Gly Leu 50 55 60 Pro Ile Ile Leu Ala Leu Ala Ser Leu Ala Asp Arg cys Gly Pro Ile 65 70 75 80 Phe Thr Ile Arg Leu Gly Ile Arg Arg Val Leu Val Val Ser Asn T rp 85 90 95 Glu Ile Ala Lys Glu Ile Phe Thr Thr His Asp Leu Ile Val Ser Asn 100 105 110 Arg Pro Lys Tyr Leu Ala Ala Lys Ile Leu Gly Phe Asn Tyr Val Ser 115 120 125 Phe Ser Phe Ala Pro Tyr Gly Pro Tyr T rp Val Gly Ile Arg Lys Ile 130 135 140 Ile Ala Thr Lys Leu Met Ser Ser Ser Arg Leu Gln Lys Leu Gln Phe 145 150 155 160 Val Arg Val Phe Glu Leu Gl u Asn Ser Met Lys Ser Ile Arg Glu Ser 165 170 175 T rp Lys Glu Lys Lys Asp Gl u Glu Gly Lys Val Leu Val Glu Met Lys 180 185 190 Lys T rp Phe T rp Glu Leu Asn Met Asn Ile Val Leu Arg Thr Val Ala 195 200 205 Gly Lys Gln Tyr Thr Gly Thr Val Asp Asp Ala Asp Ala Lys Arg Ile 210 215 220 Ser Glu Leu Phe Arg Glu Trp Phe His Tyr Thr Gly Arg Phe Val Val 225 230 235 240 Gly Asp Ala Phe Pro Phe Leu Gly T rp Leu Asp Leu Gly Gly Tyr Lys 245 250 255 Lys Thr Met Glu Leu Val Ala Ser Arg Leu Asp Ser Met Val Ser Lys 260 265 270 T rp Leu Asp Glu His Arg Lys Lys Gln Ala Asn Asp Asp Lys Lys Glu 275 280 285 Asp Met Asp Phe Met Asp Ile Met Ile Ser Met Thr Glu Ala Asn Ser Page 189

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290

295

300

Pro 305 Leu Glu Gly Tyr Gly 310 Thr Asp Thr Ile Ile 315 Lys Thr Thr cys Met 320 Thr Leu Ile Val Ser Gly Val Asp Thr Thr Ser Ile Val Leu Thr T rp 325 330 335 Ala Leu Ser Leu Leu Leu Asn Asn Arg Asp Thr Leu Lys Lys Ala Gln 340 345 350 Glu Glu Leu Asp Met cys Val Gly Lys Gly Arg Gln Val Asn Glu Ser 355 360 365 Asp Leu Val Asn Leu Ile Tyr Leu Glu Ala Val Leu Lys Glu Ala Leu 370 375 380 Arg Leu Tyr Pro Ala Ala Phe Leu Gly Gly Pro Arg Ala Phe Leu Glu 385 390 395 400 Asp cys Thr Val Ala Gly Tyr Arg Ile Pro Lys Gly Thr cys Leu Leu 405 410 415 Ile Asn Met T rp Lys Leu His Arg Asp Pro Asn Ile T rp Ser Asp Pro 420 425 430 cys Glu Phe Lys Pro Glu Arg Phe Leu Thr Pro Asn Gln Lys Asp Val 435 440 445 Asp Val Ile Gly Met Asp Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg 450 455 460 Arg Tyr cys Pro Gly Thr Arg Leu Ala Leu Gln Met Leu His Ile Val 465 470 475 480 Leu Ala Thr Leu Leu Gln Asn Phe Glu Met Ser Thr Pro Asn Asp Ala 485 490 495 Pro Val Asp Met Thr Ala Ser Val Gly Met Thr Asn Ala Lys Ala Ser 500 505 510 Pro Leu Glu Val Leu Leu Ser Pro Arg Val Lys T rp Ser 515 520 525

<210> 143 <211> 476 <212> PRT <213> Stevia rebaudiana <400> 143

Met Ile Gln Val Leu Thr Pro Ile Leu Leu Phe Leu Ile Phe Phe Val 15 10 15

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Phe T rp Lys Val Tyr Lys His Gln Lys Thr Lys Ile Asn Leu Pro Pro 20 25 30 Gly Ser Phe Gly T rp Pro Phe Leu Gly Glu Thr Leu Ala Leu Leu Arg 35 40 45 Ala Gly T rp Asp Ser Glu Pro Glu Arg Phe Val Arg Glu Arg Ile Lys 50 55 60 Lys His Gly Ser Pro Leu Val Phe Lys Thr Ser Leu Phe Gly Asp Arg 65 70 75 80 Phe Ala Val Leu cys Gly Pro Ala Gly Asn Lys Phe Leu Phe cys Asn 85 90 95 Glu Asn Lys Leu Val Ala Ser T rp T rp Pro Val Pro Val Arg Lys Leu 100 105 110 Phe Gly Lys Ser Leu Leu Thr Ile Arg Gly Asp Glu Ala Lys T rp Met 115 120 125 Arg Lys Met Leu Leu Ser Tyr Leu Gly Pro Asp Ala Phe Ala Thr His 130 135 140 Tyr Ala Val Thr Met Asp Val Val Thr Arg Arg His Ile Asp Val His 145 150 155 160 T rp Arg Gly Lys Glu Glu Val Asn Val Phe Gln Thr Val Lys Leu Tyr 165 170 175 Ala Phe Glu Leu Ala cys Arg Leu Phe Met Asn Leu Asp Asp Pro Asn 180 185 190 His Ile Ala Lys Leu Gly Ser Leu Phe Asn Ile Phe Leu Lys Gly Ile 195 200 205 Ile Glu Leu Pro Ile Asp Val Pro Gly Thr Arg Phe Tyr Ser Ser Lys 210 215 220 Lys Ala Ala Ala Ala Ile Arg Ile Glu Leu Lys Lys Leu Ile Lys Ala 225 230 235 240 Arg Lys Leu Glu Leu Lys Gl u Gly Lys Ala Ser Ser Ser Gln Asp Leu 245 250 255 Leu Ser His Leu Leu Thr Ser Pro Asp Glu Asn Gly Met Phe Leu Thr 260 265 270 Glu Glu Glu Ile Val Asp Asn Ile Leu Leu Leu Leu Phe Ala Gly His 275 280 285 Asp Thr Ser Ala Leu Ser Ile Thr Leu Leu Met Lys Thr Leu Gly Glu

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290 295 300 His Ser Asp Val Tyr Asp Lys Val Leu Lys Glu Gln Leu Glu Ile Ser 305 310 315 320 Lys Thr Lys Glu Ala T rp Gl u Ser Leu Lys T rp Glu Asp Ile Gln Lys 325 330 335 Met Lys Tyr Ser T rp Ser Val Ile cys Glu Val Met Arg Leu Asn Pro 340 345 350 Pro Val Ile Gly Thr Tyr Arg Glu Ala Leu Val Asp Ile Asp Tyr Ala 355 360 365 Gly Tyr Thr Ile Pro Lys Gly T rp Lys Leu His T rp Ser Ala Val Ser 370 375 380 Thr Gln Arg Asp Glu Ala Asn Phe Glu Asp Val Thr Arg Phe Asp Pro 385 390 395 400 Ser Arg Phe Glu Gly Ala Gly Pro Thr Pro Phe Thr Phe Val Pro Phe 405 410 415 Gly Gly Gly Pro Arg Met cys Leu Gly Lys Glu Phe Ala Arg Leu Glu 420 425 430 Val Leu Ala Phe Leu His Asn Ile Val Thr Asn Phe Lys T rp Asp Leu 435 440 445 Leu Ile Pro Asp Glu Lys Ile Glu Tyr Asp Pro Met Ala Thr Pro Ala 450 455 460 Lys Gly Leu Pro Ile Arg Leu His Pro His Gln Val

465 470 475 <210> 144 <211> 525 <212> PRT <213> Arabidopsis thaliana <400> 144

Met Glu Ser Leu Val Val His Thr Val Asn Ala Ile T rp cys Ile Val 1 5 10 15 Ile Val Gly Ile Phe Ser Val Gly Tyr His Val Tyr Gly Arg Ala Val 20 25 30 Val Glu Gln T rp Arg Met Arg Arg Ser Leu Lys Leu Gln Gly Val Lys 35 40 45 Gly Pro Pro Pro Ser Ile Phe Asn Gly Asn Val Ser Glu Met Gln Arg 50 55 60

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Ile 65 Gln Ser Glu Ala Lys 70 His cys Ser Gly Asp Asn 75 Ile Ile Ser His 80 Asp Tyr Ser Ser Ser Leu Phe Pro His Phe Asp His T rp Arg Lys Gln 85 90 95 Tyr Gly Arg Ile Tyr Thr Tyr Ser Thr Gly Leu Lys Gln His Leu Tyr 100 105 110 Ile Asn His Pro Glu Met Val Lys Glu Leu Ser Gln Thr Asn Thr Leu 115 120 125 Asn Leu Gly Arg Ile Thr His Ile Thr Lys Arg Leu Asn Pro Ile Leu 130 135 140 Gly Asn Gly Ile Ile Thr Ser Asn Gly Pro His T rp Ala His Gln Arg 145 150 155 160 Arg Ile Ile Ala Tyr Glu Phe Thr His Asp Lys Ile Lys Gly Met Val 165 170 175 Gly Leu Met Val Glu Ser Ala Met Pro Met Leu Asn Lys T rp Glu Glu 180 185 190 Met Val Lys Arg Gly Gly Gl u Met Gly cys Asp Ile Arg Val Asp Glu 195 200 205 Asp Leu Lys Asp Val Ser Ala Asp Val Ile Ala Lys Ala cys Phe Gly 210 215 220 Ser Ser Phe Ser Lys Gly Lys Ala Ile Phe Ser Met Ile Arg Asp Leu 225 230 235 240 Leu Thr Ala Ile Thr Lys Arg Ser Val Leu Phe Arg Phe Asn Gly Phe 245 250 255 Thr Asp Met Val Phe Gly Ser Lys Lys His Gly Asp Val Asp Ile Asp 260 265 270 Ala Leu Glu Met Glu Leu Gl u Ser Ser Ile T rp Glu Thr Val Lys Glu 275 280 285 Arg Glu Ile Glu cys Lys Asp Thr His Lys Lys Asp Leu Met Gln Leu 290 295 300 Ile Leu Glu Gly Ala Met Arg Ser cys Asp Gly Asn Leu T rp Asp Lys 305 310 315 320 Ser Ala Tyr Arg Arg Phe Val Val Asp Asn cys Lys Ser Ile Tyr Phe 325 330 335 Ala Gly His Asp Ser Thr Ala Val Ser Val Ser T rp cys Leu Met Leu

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340 345 350 Leu Ala Leu Asn Pro Ser Trp Gln Val Lys Ile Arg Asp Glu Ile Leu 355 360 365 Ser Ser cys Lys Asn Gly Ile Pro Asp Ala Glu Ser Ile Pro Asn Leu 370 375 380 Lys Thr Val Thr Met Val Ile Gln Glu Thr Met Arg Leu Tyr Pro Pro 385 390 395 400 Ala Pro Ile Val Gly Arg Gl u Ala Ser Lys Asp Ile Arg Leu Gly Asp 405 410 415 Leu Val Val Pro Lys Gly Val cys Ile T rp Thr Leu Ile Pro Ala Leu 420 425 430 His Arg Asp Pro Glu Ile Trp Gly Pro Asp Ala Asn Asp Phe Lys Pro 435 440 445 Glu Arg Phe Ser Glu Gly Ile Ser Lys Ala cys Lys Tyr Pro Gln Ser 450 455 460 Tyr Ile Pro Phe Gly Leu Gly Pro Arg Thr cys Val Gly Lys Asn Phe 465 470 475 480 Gly Met Met Glu Val Lys Val Leu Val Ser Leu Ile Val Ser Lys Phe 485 490 495 Ser Phe Thr Leu Ser Pro Thr Tyr Gln His Ser Pro Ser His Lys Leu 500 505 510 Leu Val Glu Pro Gln His Gly Val Val Ile Arg Val Val

515 520 525 <210> 145 <211> 529 <212> PRT

<213> V tis vin fera <400> 145 Met Tyr Phe Leu Leu Gln Tyr Leu Asn Ile Thr Thr Val Gly Val Phe 1 5 10 15 Ala Thr Leu Phe Leu Ser Tyr cys Leu Leu Leu T rp Arg Ser Arg Ala 20 25 30 Gly Asn Lys Lys Ile Ala Pro Glu Ala Ala Ala Ala T rp Pro Ile Ile 35 40 45 Gly His Leu His Leu Leu Ala Gly Gly Ser His Gln Leu Pro His Ile 50 55 60

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Thr 65 Leu Gly Asn Met Ala Asp Lys Tyr Gly Pro Val Phe Thr Ile Arg 80 70 75 Ile Gly Leu His Arg Ala Val Val Val Ser Ser T rp Glu Met Ala Lys 85 90 95 Glu cys Ser Thr Ala Asn Asp Gln Val Ser Ser Ser Arg Pro Glu Leu 100 105 110 Leu Ala Ser Lys Leu Leu Gly Tyr Asn Tyr Ala Met Phe Gly Phe Ser 115 120 125 Pro Tyr Gly Ser Tyr T rp Arg Glu Met Arg Lys Ile Ile Ser Leu Glu 130 135 140 Leu Leu Ser Asn Ser Arg Leu Glu Leu Leu Lys Asp Val Arg Ala Ser 145 150 155 160 Glu Val Val Thr Ser Ile Lys Glu Leu Tyr Lys Leu T rp Ala Glu Lys 165 170 175 Lys Asn Glu Ser Gly Leu Val Ser Val Glu Met Lys Gln T rp Phe Gly 180 185 190 Asp Leu Thr Leu Asn Val Ile Leu Arg Met Val Ala Gly Lys Arg Tyr 195 200 205 Phe Ser Ala Ser Asp Ala Ser Glu Asn Lys Gln Ala Gln Arg cys Arg 210 215 220 Arg Val Phe Arg Glu Phe Phe His Leu Ser Gly Leu Phe Val Val Ala 225 230 235 240 Asp Ala Ile Pro Phe Leu Gly T rp Leu Asp T rp Gly Arg His Glu Lys 245 250 255 Thr Leu Lys Lys Thr Ala Ile Glu Met Asp Ser Ile Ala Gln Glu T rp 260 265 270 Leu Glu Glu His Arg Arg Arg Lys Asp Ser Gly Asp Asp Asn Ser Thr 275 280 285 Gln Asp Phe Met Asp Val Met Gln Ser Val Leu Asp Gly Lys Asn Leu 290 295 300 Gly Gly Tyr Asp Ala Asp Thr Ile Asn Lys Ala Thr cys Leu Thr Leu 305 310 315 320 Ile Ser Gly Gly Ser Asp Thr Thr Val Val Ser Leu Thr T rp Ala Leu 325 330 335 Ser Leu Val Leu Asn Asn Arg Asp Thr Leu Lys Lys Ala Gln Glu Glu Page 195

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340

345

350

Leu Asp Ile Gln 355 Val Gly Lys Glu 360 Arg Leu Val Asn Glu 365 Gln Asp Ile Ser Lys Leu Val Tyr Leu Gl n Ala Ile Val Lys Glu Thr Leu Arg Leu 370 375 380 Tyr Pro Pro Gly Pro Leu Gly Gly Leu Arg Gln Phe Thr Glu Asp cys 385 390 395 400 Thr Leu Gly Gly Tyr His Val Ser Lys Gly Thr Arg Leu Ile Met Asn 405 410 415 Leu Ser Lys Ile Gln Lys Asp Pro Arg Ile T rp Ser Asp Pro Thr Glu 420 425 430 Phe Gln Pro Glu Arg Phe Leu Thr Thr His Lys Asp Val Asp Pro Arg 435 440 445 Gly Lys His Phe Glu Phe Ile Pro Phe Gly Ala Gly Arg Arg Ala cys 450 455 460 Pro Gly Ile Thr Phe Gly Leu Gln Val Leu His Leu Thr Leu Ala Ser 465 470 475 480 Phe Leu His Ala Phe Glu Phe Ser Thr Pro Ser Asn Glu Gln Val Asn 485 490 495 Met Arg Glu Ser Leu Gly Leu Thr Asn Met Lys Ser Thr Pro Leu Glu 500 505 510 Val Leu Ile Ser Pro Arg Leu Ser Leu Asn cys Phe Asn Leu Met Lys 515 520 525

Ile <210> 146 <211> 479 <212> PRT <213> Medicago trunculata <400> 146

Met 1 Glu Pro Asn Phe 5 Tyr Leu Ser Leu Leu 10 Leu Leu Phe Val Thr 15 Phe Ile Ser Leu Ser Leu Phe Phe Ile Phe Tyr Lys Gln Lys Ser Pro Leu 20 25 30 Asn Leu Pro Pro Gly Lys Met Gly Tyr Pro Ile Ile Gly Glu Ser Leu 35 40 45

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Glu Phe 50 Leu Ser Thr Gly Trp 55 Lys Gly His Pro Glu 60 Lys Phe Ile Phe Asp Arg Met Arg Lys Tyr Ser Ser Glu Leu Phe Lys Thr Ser Ile Val 65 70 75 80 Gly Glu Ser Thr Val Val cys cys Gly Ala Ala Ser Asn Lys Phe Leu 85 90 95 Phe Ser Asn Glu Asn Lys Leu Val Thr Ala T rp T rp Pro Asp Ser Val 100 105 110 Asn Lys Ile Phe Pro Thr Thr Ser Leu Asp Ser Asn Leu Lys Glu Glu 115 120 125 Ser Ile Lys Met Arg Lys Leu Leu Pro Gln Phe Phe Lys Pro Glu Ala 130 135 140 Leu Gln Arg Tyr Val Gly Val Met Asp Val Ile Ala Gln Arg His Phe 145 150 155 160 Val Thr His T rp Asp Asn Lys Asn Glu Ile Thr Val Tyr Pro Leu Ala 165 170 175 Lys Arg Tyr Thr Phe Leu Leu Ala cys Arg Leu Phe Met Ser Val Glu 180 185 190 Asp Glu Asn His Val Ala Lys Phe Ser Asp Pro Phe Gln Leu Ile Ala 195 200 205 Ala Gly Ile Ile Ser Leu Pro Ile Asp Leu Pro Gly Thr Pro Phe Asn 210 215 220 Lys Ala Ile Lys Ala Ser Asn Phe Ile Arg Lys Glu Leu Ile Lys Ile 225 230 235 240 Ile Lys Gln Arg Arg Val Asp Leu Ala Glu Gly Thr Ala Ser Pro Thr 245 250 255 Gln Asp Ile Leu Ser His Met Leu Leu Thr Ser Asp Glu Asn Gly Lys 260 265 270 Ser Met Asn Glu Leu Asn Ile Ala Asp Lys Ile Leu Gly Leu Leu Ile 275 280 285 Gly Gly His Asp Thr Ala Ser Val Ala cys Thr Phe Leu Val Lys Tyr 290 295 300 Leu Gly Glu Leu Pro His Ile Tyr Asp Lys Val Tyr Gln Glu Gln Met 305 310 315 320 Glu Ile Ala Lys Ser Lys Pro Ala Gly Glu Leu Leu Asn T rp Asp Asp Page 197

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325 330 335 Leu Lys Lys Met Lys Tyr Ser T rp Asn Val Ala Cys Glu Val Met Arg 340 345 350 Leu Ser Pro Pro Leu Gln Gly Gly Phe Arg Glu Ala Ile Thr Asp Phe 355 360 365 Met Phe Asn Gly Phe Ser Ile Pro Lys Gly T rp Lys Leu Tyr T rp Ser 370 375 380 Ala Asn Ser Thr His Lys Asn Ala Glu Cys Phe Pro Met Pro Glu Lys 385 390 395 400 Phe Asp Pro Thr Arg Phe Gl u Gly Asn Gly Pro Ala Pro Tyr Thr Phe 405 410 415 Val Pro Phe Gly Gly Gly Pro Arg Met Cys Pro Gly Lys Glu Tyr Ala 420 425 430 Arg Leu Glu Ile Leu Val Phe Met His Asn Leu Val Lys Arg Phe Lys 435 440 445 T rp Glu Lys Val Ile Pro Asp Glu Lys Ile Ile Val Asp Pro Phe Pro 450 455 460 Ile Pro Ala Lys Asp Leu Pro Ile Arg Leu Tyr Pro His Lys Ala

465 470 475 <210> 147 <211> 710 <212> PRT <213> Stevia rebaudiana <400> 147

Met 1 Gln Ser Asp Ser 5 Val Lys Val Ser Pro 10 Phe Asp Leu Val Ser 15 Ala Ala Met Asn Gly Lys Ala Met Glu Lys Leu Asn Ala Ser Glu Ser Glu 20 25 30 Asp Pro Thr Thr Leu Pro Ala Leu Lys Met Leu Val Glu Asn Arg Glu 35 40 45 Leu Leu Thr Leu Phe Thr Thr Ser Phe Ala Val Leu Ile Gly Cys Leu 50 55 60 Val Phe Leu Met T rp Arg Arg Ser Ser Ser Lys Lys Leu Val Gln Asp 65 70 75 80 Pro Val Pro Gln Val Ile Val Val Lys Lys Lys Glu Lys Glu Ser Glu 85 90 95

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Val Asp Asp Gly 100 Lys Lys Lys Val Ser Ile 105 Phe Tyr Gly Thr Gln Thr 110 Gly Thr Ala Glu Gly Phe Ala Lys Ala Leu Val Glu Glu Ala Lys Val 115 120 125 Arg Tyr Glu Lys Thr Ser Phe Lys Val Ile Asp Leu Asp Asp Tyr Ala 130 135 140 Ala Asp Asp Asp Glu Tyr Gl u Glu Lys Leu Lys Lys Glu Ser Leu Ala 145 150 155 160 Phe Phe Phe Leu Ala Thr Tyr Gly Asp Gly Glu Pro Thr Asp Asn Ala 165 170 175 Ala Asn Phe Tyr Lys T rp Phe Thr Glu Gly Asp Asp Lys Gly Glu T rp 180 185 190 Leu Lys Lys Leu Gln Tyr Gly Val Phe Gly Leu Gly Asn Arg Gln Tyr 195 200 205 Glu His Phe Asn Lys Ile Ala Ile Val Val Asp Asp Lys Leu Thr Glu 210 215 220 Met Gly Ala Lys Arg Leu Val Pro Val Gly Leu Gly Asp Asp Asp Gln 225 230 235 240 cys Ile Glu Asp Asp Phe Thr Ala T rp Lys Glu Leu Val T rp Pro Glu 245 250 255 Leu Asp Gln Leu Leu Arg Asp Glu Asp Asp Thr Ser Val Thr Thr Pro 260 265 270 Tyr Thr Ala Ala Val Leu Gl u Tyr Arg Val Val Tyr His Asp Lys Pro 275 280 285 Ala Asp Ser Tyr Ala Glu Asp Gln Thr His Thr Asn Gly His Val Val 290 295 300 His Asp Ala Gln His Pro Ser Arg Ser Asn Val Ala Phe Lys Lys Glu 305 310 315 320 Leu His Thr Ser Gln Ser Asp Arg Ser cys Thr His Leu Glu Phe Asp 325 330 335 Ile Ser His Thr Gly Leu Ser Tyr Glu Thr Gly Asp His Val Gly Val 340 345 350 Tyr Ser Glu Asn Leu Ser Gl u Val Val Asp Glu Ala Leu Lys Leu Leu 355 360 365 Gly Leu Ser Pro Asp Thr Tyr Phe Ser Val His Ala Asp Lys Glu Asp Page 199

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370 375 380

Gly 385 Thr Pro Ile Gly Gly Ala 390 Ser Leu Pro Pro 395 Pro Phe Pro Pro cys 400 Thr Leu Arg Asp Ala Leu Thr Arg Tyr Ala Asp Val Leu Ser Ser Pro 405 410 415 Lys Lys Val Ala Leu Leu Ala Leu Ala Ala His Ala Ser Asp Pro Ser 420 425 430 Glu Ala Asp Arg Leu Lys Phe Leu Ala Ser Pro Ala Gly Lys Asp Glu 435 440 445 Tyr Ala Gln T rp Ile Val Ala Asn Gln Arg Ser Leu Leu Glu Val Met 450 455 460 Gln Ser Phe Pro Ser Ala Lys Pro Pro Leu Gly Val Phe Phe Ala Ala 465 470 475 480 Val Ala Pro Arg Leu Gln Pro Arg Tyr Tyr Ser Ile Ser Ser Ser Pro 485 490 495 Lys Met Ser Pro Asn Arg Ile His Val Thr cys Ala Leu Val Tyr Glu 500 505 510 Thr Thr Pro Ala Gly Arg Ile His Arg Gly Leu cys Ser Thr T rp Met 515 520 525 Lys Asn Ala Val Pro Leu Thr Glu Ser Pro Asp cys Ser Gln Ala Ser 530 535 540 Ile Phe Val Arg Thr Ser Asn Phe Arg Leu Pro Val Asp Pro Lys Val 545 550 555 560 Pro Val Ile Met Ile Gly Pro Gly Thr Gly Leu Ala Pro Phe Arg Gly 565 570 575 Phe Leu Gln Glu Arg Leu Ala Leu Lys Glu Ser Gly Thr Glu Leu Gly 580 585 590 Ser Ser Ile Phe Phe Phe Gly cys Arg Asn Arg Lys Val Asp Phe Ile 595 600 605 Tyr Glu Asp Glu Leu Asn Asn Phe Val Glu Thr Gly Ala Leu Ser Glu 610 615 620 Leu Ile Val Ala Phe Ser Arg Glu Gly Thr Ala Lys Glu Tyr Val Gln 625 630 635 640 His Lys Met Ser Gln Lys Ala Ser Asp Ile T rp Lys Leu Leu Ser Glu 645 650 655

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Gly Ala Tyr Leu Tyr Val cys Gly Asp Ala Lys Gly Met Ala Lys Asp 660 665 670 Val His Arg Thr Leu His Thr Ile Val Gln Glu Gln Gly Ser Leu Asp 675 680 685 Ser Ser Lys Ala Glu Leu Tyr Val Lys Asn Leu Gln Met Ser Gly Arg 690 695 700 Tyr Leu Arg Asp Val T rp

705 710 <210> 148 <211> 692 <212> PRT <213> Arabidopsis thaliana <400> 148

Met Thr Ser Ala Leu Tyr Ala Ser Asp Leu 10 Phe Lys Gln Leu Lys 15 Ser 1 5 Ile Met Gly Thr Asp Ser Leu Ser Asp Asp Val Val Leu Val Ile Ala 20 25 30 Thr Thr Ser Leu Ala Leu Val Ala Gly Phe Val Val Leu Leu T rp Lys 35 40 45 Lys Thr Thr Ala Asp Arg Ser Gly Glu Leu Lys Pro Leu Met Ile Pro 50 55 60 Lys Ser Leu Met Ala Lys Asp Glu Asp Asp Asp Leu Asp Leu Gly Ser 65 70 75 80 Gly Lys Thr Arg Val Ser Ile Phe Phe Gly Thr Gln Thr Gly Thr Ala 85 90 95 Glu Gly Phe Ala Lys Ala Leu Ser Glu Glu Ile Lys Ala Arg Tyr Glu 100 105 110 Lys Ala Ala Val Lys Val Ile Asp Leu Asp Asp Tyr Ala Ala Asp Asp 115 120 125 Asp Gln Tyr Glu Glu Lys Leu Lys Lys Glu Thr Leu Ala Phe Phe cys 130 135 140 Val Ala Thr Tyr Gly Asp Gly Glu Pro Thr Asp Asn Ala Ala Arg Phe 145 150 155 160 Tyr Lys T rp Phe Thr Glu Gl u Asn Glu Arg Asp Ile Lys Leu Gln Gln 165 170 175 Leu Ala Tyr Gly Val Phe Ala Leu Gly Asn Arg Gln Tyr Glu His Phe

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180 185 190 Asn Lys Ile Gly Ile Val Leu Asp Glu Glu Leu cys Lys Lys Gly Ala 195 200 205 Lys Arg Leu Ile Glu Val Gly Leu Gly Asp Asp Asp Gln Ser Ile Glu 210 215 220 Asp Asp Phe Asn Ala T rp Lys Glu Ser Leu T rp Ser Glu Leu Asp Lys 225 230 235 240 Leu Leu Lys Asp Glu Asp Asp Lys Ser Val Ala Thr Pro Tyr Thr Ala 245 250 255 Val Ile Pro Glu Tyr Arg Val Val Thr His Asp Pro Arg Phe Thr Thr 260 265 270 Gln Lys Ser Met Glu Ser Asn Val Ala Asn Gly Asn Thr Thr Ile Asp 275 280 285 Ile His His Pro cys Arg Val Asp Val Ala Val Gln Lys Glu Leu His 290 295 300 Thr His Glu Ser Asp Arg Ser cys Ile His Leu Glu Phe Asp Ile Ser 305 310 315 320 Arg Thr Gly Ile Thr Tyr Gl u Thr Gly Asp His Val Gly Val Tyr Ala 325 330 335 Glu Asn His Val Glu Ile Val Glu Glu Ala Gly Lys Leu Leu Gly His 340 345 350 Ser Leu Asp Leu Val Phe Ser Ile His Ala Asp Lys Glu Asp Gly Ser 355 360 365 Pro Leu Glu Ser Ala Val Pro Pro Pro Phe Pro Gly Pro cys Thr Leu 370 375 380 Gly Thr Gly Leu Ala Arg Tyr Ala Asp Leu Leu Asn Pro Pro Arg Lys 385 390 395 400 Ser Ala Leu Val Ala Leu Ala Ala Tyr Ala Thr Glu Pro Ser Glu Ala 405 410 415 Glu Lys Leu Lys His Leu Thr Ser Pro Asp Gly Lys Asp Glu Tyr Ser 420 425 430 Gln T rp Ile Val Ala Ser Gl n Arg Ser Leu Leu Glu Val Met Ala Ala 435 440 445 Phe Pro Ser Ala Lys Pro Pro Leu Gly Val Phe Phe Ala Ala Ile Ala

450 455 460

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Pro Arg 465 Leu Gln Pro Arg 470 Tyr Tyr Ser Ile Ser 475 Ser Ser Pro Arg Leu 480 Ala Pro Ser Arg Val His Val Thr Ser Ala Leu Val Tyr Gly Pro Thr 485 490 495 Pro Thr Gly Arg Ile His Lys Gly Val cys Ser Thr T rp Met Lys Asn 500 505 510 Ala Val Pro Ala Glu Lys Ser His Glu cys Ser Gly Ala Pro Ile Phe 515 520 525 Ile Arg Ala Ser Asn Phe Lys Leu Pro Ser Asn Pro Ser Thr Pro Ile 530 535 540 Val Met Val Gly Pro Gly Thr Gly Leu Ala Pro Phe Arg Gly Phe Leu 545 550 555 560 Gln Glu Arg Met Ala Leu Lys Glu Asp Gly Glu Glu Leu Gly Ser Ser 565 570 575 Leu Leu Phe Phe Gly cys Arg Asn Arg Gln Met Asp Phe Ile Tyr Glu 580 585 590 Asp Glu Leu Asn Asn Phe Val Asp Gln Gly Val Ile Ser Glu Leu Ile 595 600 605 Met Ala Phe Ser Arg Glu Gly Ala Gln Lys Glu Tyr Val Gln His Lys 610 615 620 Met Met Glu Lys Ala Ala Gl n Val T rp Asp Leu Ile Lys Glu Glu Gly 625 630 635 640 Tyr Leu Tyr Val cys Gly Asp Ala Lys Gly Met Ala Arg Asp Val His 645 650 655 Arg Thr Leu His Thr Ile Val Gln Glu Gln Glu Gly Val Ser Ser Ser 660 665 670 Glu Ala Glu Ala Ile Val Lys Lys Leu Gln Thr Glu Gly Arg Tyr Leu 675 680 685

Arg Asp Val Trp 690 <210> 149 <211> 713 <212> PRT <213> Giberella fujikuroi <400> 149

Met Ala Glu Leu Asp Thr Leu Asp Ile Val Val Leu Gly Val Ile Phe Page 203

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Leu Gly Thr Val Ala Tyr Phe Thr Lys Gly Lys Leu T rp Gly Val Thr 20 25 30 Lys Asp Pro Tyr Ala Asn Gly Phe Ala Ala Gly Gly Ala Ser Lys Pro 35 40 45 Gly Arg Thr Arg Asn Ile Val Glu Ala Met Glu Glu Ser Gly Lys Asn 50 55 60 cys Val Val Phe Tyr Gly Ser Gln Thr Gly Thr Ala Glu Asp Tyr Ala 65 70 75 80 Ser Arg Leu Ala Lys Glu Gly Lys Ser Arg Phe Gly Leu Asn Thr Met 85 90 95 Ile Ala Asp Leu Glu Asp Tyr Asp Phe Asp Asn Leu Asp Thr Val Pro 100 105 110 Ser Asp Asn Ile Val Met Phe Val Leu Ala Thr Tyr Gly Glu Gly Glu 115 120 125 Pro Thr Asp Asn Ala Val Asp Phe Tyr Glu Phe Ile Thr Gly Glu Asp 130 135 140 Ala Ser Phe Asn Glu Gly Asn Asp Pro Pro Leu Gly Asn Leu Asn Tyr 145 150 155 160 Val Ala Phe Gly Leu Gly Asn Asn Thr Tyr Glu His Tyr Asn Ser Met 165 170 175 Val Arg Asn Val Asn Lys Ala Leu Glu Lys Leu Gly Ala His Arg Ile 180 185 190 Gly Glu Ala Gly Glu Gly Asp Asp Gly Ala Gly Thr Met Glu Glu Asp 195 200 205 Phe Leu Ala T rp Lys Asp Pro Met T rp Glu Ala Leu Ala Lys Lys Met 210 215 220 Gly Leu Glu Glu Arg Glu Ala Val Tyr Glu Pro Ile Phe Ala Ile Asn 225 230 235 240 Glu Arg Asp Asp Leu Thr Pro Glu Ala Asn Glu Val Tyr Leu Gly Glu 245 250 255 Pro Asn Lys Leu His Leu Gl u Gly Thr Ala Lys Gly Pro Phe Asn Ser 260 265 270 His Asn Pro Tyr Ile Ala Pro Ile Ala Glu Ser Tyr Glu Leu Phe Ser

275

280

285

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Ala Lys Asp Arg Asn cys Leu His Met Glu Ile Asp Ile Ser Gly Ser 290 295 300 Asn Leu Lys Tyr Glu Thr Gly Asp His Ile Ala Ile T rp Pro Thr Asn 305 310 315 320 Pro Gly Glu Glu Val Asn Lys Phe Leu Asp Ile Leu Asp Leu Ser Gly 325 330 335 Lys Gln His Ser Val Val Thr Val Lys Ala Leu Glu Pro Thr Ala Lys 340 345 350 Val Pro Phe Pro Asn Pro Thr Thr Tyr Asp Ala Ile Leu Arg Tyr His 355 360 365 Leu Glu Ile cys Ala Pro Val Ser Arg Gln Phe Val Ser Thr Leu Ala 370 375 380 Ala Phe Ala Pro Asn Asp Asp Ile Lys Ala Glu Met Asn Arg Leu Gly 385 390 395 400 Ser Asp Lys Asp Tyr Phe His Glu Lys Thr Gly Pro His Tyr Tyr Asn 405 410 415 Ile Ala Arg Phe Leu Ala Ser Val Ser Lys Gly Glu Lys T rp Thr Lys 420 425 430 Ile Pro Phe Ser Ala Phe Ile Glu Gly Leu Thr Lys Leu Gln Pro Arg 435 440 445 Tyr Tyr Ser Ile Ser Ser Ser Ser Leu Val Gln Pro Lys Lys Ile Ser 450 455 460 Ile Thr Ala Val Val Glu Ser Gln Gln Ile Pro Gly Arg Asp Asp Pro 465 470 475 480 Phe Arg Gly Val Ala Thr Asn Tyr Leu Phe Ala Leu Lys Gln Lys Gln 485 490 495 Asn Gly Asp Pro Asn Pro Ala Pro Phe Gly Gln Ser Tyr Glu Leu Thr 500 505 510 Gly Pro Arg Asn Lys Tyr Asp Gly Ile His Val Pro Val His Val Arg 515 520 525 His Ser Asn Phe Lys Leu Pro Ser Asp Pro Gly Lys Pro Ile Ile Met 530 535 540 Ile Gly Pro Gly Thr Gly Val Ala Pro Phe Arg Gly Phe Val Gln Glu 545 550 555 560

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Arg Ala Lys Gln Ala Arg Asp Gly Val Glu Val 570 Gly Lys Thr Leu 575 Leu 565 Phe Phe Gly cys Arg Lys Ser Thr Glu Asp Phe Met Tyr Gln Lys Glu 580 585 590 T rp Gln Glu Tyr Lys Glu Ala Leu Gly Asp Lys Phe Glu Met Ile Thr 595 600 605 Ala Phe Ser Arg Glu Gly Ser Lys Lys Val Tyr Val Gln His Arg Leu 610 615 620 Lys Glu Arg Ser Lys Glu Val Ser Asp Leu Leu Ser Gln Lys Ala Tyr 625 630 635 640 Phe Tyr Val cys Gly Asp Ala Ala His Met Ala Arg Glu Val Asn Thr 645 650 655 Val Leu Ala Gln Ile Ile Ala Glu Gly Arg Gly Val Ser Glu Ala Lys 660 665 670 Gly Glu Glu Ile Val Lys Asn Met Arg Ser Ala Asn Gln Tyr Gln Val 675 680 685 cys Ser Asp Phe Val Thr Leu His cys Lys Glu Thr Thr Tyr Ala Asn 690 695 700 Ser Glu Leu Gln Glu Asp Val T rp Ser

705 710 <210> 150 <211> 453 <212> PRT <213> Arabidopsis thaliana <400> 150

Met 1 Gly Gly Leu Lys 5 Phe His Val Leu Met 10 Tyr Pro Trp Phe Ala 15 Thr Gly His Met Thr Pro Phe Leu Phe Leu Ala Asn Lys Leu Ala Glu Lys 20 25 30 Gly His Thr Val Thr Phe Leu Leu Pro Lys Lys Ser Leu Lys Gln Leu 35 40 45 Glu His Phe Asn Leu Phe Pro His Asn Ile Val Phe Arg Ser Val Thr 50 55 60 Val Pro His Val Asp Gly Leu Pro Val Gly Thr Glu Thr Ala Ser Glu 65 70 75 80 Ile Pro Val Thr Ser Thr Asp Leu Leu Met Ser Ala Met Asp Leu Thr 85 90 95

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Arg Asp Gln Val 100 Glu Ala Val Val Arg 105 Ala Val Glu Pro Asp 110 Leu Ile Phe Phe Asp Phe Ala His Trp Ile Pro Glu Val Ala Arg Asp Phe Gly 115 120 125 Leu Lys Thr Val Lys Tyr Val Val Val Ser Ala Ser Thr Ile Ala Ser 130 135 140 Met Leu Val Pro Gly Gly Gl u Leu Gly Val Pro Pro Pro Gly Tyr Pro 145 150 155 160 Ser Ser Lys Val Leu Leu Arg Lys Gln Asp Ala Tyr Thr Met Lys Lys 165 170 175 Leu Glu Pro Thr Asn Thr Ile Asp Val Gly Pro Asn Leu Leu Glu Arg 180 185 190 Val Thr Thr Ser Leu Met Asn Ser Asp Val Ile Ala Ile Arg Thr Ala 195 200 205 Arg Glu Ile Glu Gly Asn Phe cys Asp Tyr Ile Glu Lys His cys Arg 210 215 220 Lys Lys Val Leu Leu Thr Gly Pro Val Phe Pro Glu Pro Asp Lys Thr 225 230 235 240 Arg Glu Leu Glu Glu Arg Trp Val Lys T rp Leu Ser Gly Tyr Glu Pro 245 250 255 Asp Ser Val Val Phe cys Ala Leu Gly Ser Gln Val Ile Leu Glu Lys 260 265 270 Asp Gln Phe Gln Glu Leu cys Leu Gly Met Glu Leu Thr Gly Ser Pro 275 280 285 Phe Leu Val Ala Val Lys Pro Pro Arg Gly Ser Ser Thr Ile Gln Glu 290 295 300 Ala Leu Pro Glu Gly Phe Gl u Glu Arg Val Lys Gly Arg Gly Leu Val 305 310 315 320 T rp Gly Gly T rp Val Gln Gl n Pro Leu Ile Leu Ser His Pro Ser Val 325 330 335 Gly cys Phe Val Ser His cys Gly Phe Gly Ser Met T rp Glu Ser Leu 340 345 350 Leu Ser Asp cys Gln Ile Val Leu Val Pro Gln Leu Gly Asp Gln Val 355 360 365

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Leu Asn Thr Arg 370 Leu Leu Ser Asp 375 Glu Leu Lys Val 380 Ser Val Glu Val Ala Arg Glu Glu Thr Gly Trp Phe Ser Lys Glu Ser Leu cys Asp Ala 385 390 395 400 Val Asn Ser Val Met Lys Arg Asp Ser Glu Leu Gly Asn Leu Val Arg 405 410 415 Lys Asn His Thr Lys T rp Arg Glu Thr Val Ala Ser Pro Gly Leu Met 420 425 430 Thr Gly Tyr Val Asp Ala Phe Val Glu Ser Leu Gln Asp Leu Val Ser 435 440 445

Gly Thr Thr His Asp 450 <210> 151 <211> 1362 <212> DNA <213> Arabidopsis thaliana

<400> 151 atgggtggtt tgaagtttca tgtacttatg tatccatggt tcgcaacagg ccatatgacc 60 ccgttccttt ttcttgccaa caaattggct gagaaaggtc atacggtcac tttcttgctt 120 cccaagaaat ctctgaaaca gttggaacat ttcaatctgt ttccacacaa cattgtcttt 180 cgctctgtca ccgtccctca tgtggatggt ctccccgttg gcacagagac agcctctgag 240 atccctgtga catcaactga tctcttgatg tctgctatgg atctcacacg tgatcaagtt 300 gaagctgtgg tccgagccgt tgaaccggac ctgatcttct ttgactttgc tcattggatt 360 ccagaagtag ctagggactt cggccttaag actgtaaagt acgtcgtggt gtctgcatcg 420 actatagcta gtatgcttgt cccaggtggt gagttaggtg ttcctccacc gggatatcca 480 tcatcaaagg tgctgcttcg taaacaagat gcttacacta tgaagaaact ggagcctaca 540 aatacaatcg atgtcggacc aaacctcttg gaacgagtca ctacaagtct tatgaactct 600 gatgtcattg cgataaggac agccagagaa atcgaaggaa acttttgcga ctatatagaa 660 aaacattgca ggaaaaaggt tctcttgaca ggtccggtgt tccctgagcc agacaagact 720 agagagctag aggaacgatg ggttaagtgg ctaagtgggt atgaaccaga ctcagtggtg 780 ttttgtgcac tgggctcaca agtcatttta gagaaagatc aattccaaga actctgctta 840 ggaatggagc taacaggttc accgtttctt gtagcggtta agccccctag aggctcatca 900 acgattcaag aagcacttcc tgaaggattc gaagagcggg ttaaaggaag aggccttgtt 960 tggggaggat gggttcaaca accattgata ttgtctcatc catcagtcgg gtgctttgtg 1020 agccattgtg ggtttggatc aatgtgggag tctttgctga gtgattgtca gatagtctta 1080 gtaccacagt tgggtgatca agtcctgaac acaagattgc tgagtgacga actcaaggtt 1140 Page 208

2018200459 19 Jan 2018 tcggttgaag tggcaagaga ggaaacagga tggttctcga aagagagctt gtgcgatgct 1200 gtcaatagtg tgatgaaaag ggacagcgag ctcgggaacc tggtgaggaa gaatcacacc 1260 aagtggaggg agacagtagc tagtcctgga ctaatgactg gttatgtcga tgctttcgta 1320 gagtcattgc aggatcttgt ctctgggacc acccatgact ga 1362

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