JP6560214B2 - プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法 - Google Patents

プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法 Download PDF

Info

Publication number
JP6560214B2
JP6560214B2 JP2016533121A JP2016533121A JP6560214B2 JP 6560214 B2 JP6560214 B2 JP 6560214B2 JP 2016533121 A JP2016533121 A JP 2016533121A JP 2016533121 A JP2016533121 A JP 2016533121A JP 6560214 B2 JP6560214 B2 JP 6560214B2
Authority
JP
Japan
Prior art keywords
amylase
amino acid
enzyme
starch
pcuamy1
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2016533121A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016538860A5 (https=
JP2016538860A (ja
Inventor
ファイナン、ディナ
Original Assignee
ダニスコ・ユーエス・インク
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ダニスコ・ユーエス・インク filed Critical ダニスコ・ユーエス・インク
Publication of JP2016538860A publication Critical patent/JP2016538860A/ja
Publication of JP2016538860A5 publication Critical patent/JP2016538860A5/ja
Application granted granted Critical
Publication of JP6560214B2 publication Critical patent/JP6560214B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001Alpha-amylase (3.2.1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2016533121A 2013-11-20 2014-11-13 プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法 Expired - Fee Related JP6560214B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361906617P 2013-11-20 2013-11-20
US61/906,617 2013-11-20
PCT/US2014/065522 WO2015077126A1 (en) 2013-11-20 2014-11-13 Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof

Publications (3)

Publication Number Publication Date
JP2016538860A JP2016538860A (ja) 2016-12-15
JP2016538860A5 JP2016538860A5 (https=) 2017-12-07
JP6560214B2 true JP6560214B2 (ja) 2019-08-14

Family

ID=52023630

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016533121A Expired - Fee Related JP6560214B2 (ja) 2013-11-20 2014-11-13 プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法

Country Status (7)

Country Link
US (1) US20160272957A1 (https=)
EP (1) EP3071691B1 (https=)
JP (1) JP6560214B2 (https=)
CN (1) CN105960456A (https=)
BR (1) BR112016010551A2 (https=)
MX (1) MX2016006489A (https=)
WO (1) WO2015077126A1 (https=)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3354728B1 (en) * 2012-12-21 2020-04-22 Danisco US Inc. Alpha-amylase variants
EP3259358B1 (en) * 2015-02-19 2024-06-19 Danisco US Inc. Enhanced protein expression
EP4219704A3 (en) 2015-05-13 2023-08-23 Danisco US Inc Aprl-clade protease variants and uses thereof
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
WO2016201069A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc Low-density enzyme-containing particles
EP3307427B1 (en) 2015-06-09 2023-08-16 Danisco US Inc. Osmotic burst encapsulates
JP7015695B2 (ja) 2015-06-17 2022-02-03 ダニスコ・ユーエス・インク バチルス・ギブソニイ(Bacillus gibsonii)クレードセリンプロテアーゼ
CN108603183B (zh) 2015-11-05 2023-11-03 丹尼斯科美国公司 类芽孢杆菌属物种和芽孢杆菌属物种甘露聚糖酶
EP3371308B1 (en) 2015-11-05 2022-05-11 Danisco US Inc. Paenibacillus sp. mannanases
EP3387124B1 (en) * 2015-12-09 2021-05-19 Danisco US Inc. Alpha-amylase combinatorial variants
US20180362946A1 (en) 2015-12-18 2018-12-20 Danisco Us Inc. Polypeptides with endoglucanase activity and uses thereof
DE102016201643A1 (de) * 2016-02-03 2017-08-03 Henkel Ag & Co. Kgaa Verbesserte Waschleistung durch eine alpha-Amylase aus Bacillus cereus
BR112018072530A2 (pt) 2016-05-03 2019-03-26 Danisco Us Inc variantes de protease e usos das mesmas
BR112018072586A2 (pt) 2016-05-05 2019-02-19 Danisco Us Inc variantes de protease e usos das mesmas
EP3455352B1 (en) * 2016-05-09 2026-03-18 Novozymes A/S Variant polypeptides with improved performance and use of the same
CA3026064A1 (en) 2016-05-31 2017-12-07 Danisco Us Inc. Protease variants and uses thereof
CA3027745A1 (en) 2016-06-17 2017-12-21 Danisco Us Inc. Protease variants and uses thereof
WO2018085524A2 (en) 2016-11-07 2018-05-11 Danisco Us Inc Laundry detergent composition
US11946081B2 (en) 2016-12-21 2024-04-02 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
EP4520820A3 (en) 2016-12-21 2025-04-16 Danisco Us Inc Protease variants and uses thereof
EP3583210B1 (en) 2017-03-15 2021-07-07 Danisco US Inc. Trypsin-like serine proteases and uses thereof
US20200040283A1 (en) 2017-03-31 2020-02-06 Danisco Us Inc Delayed release enzyme formulations for bleach-containing detergents
DK3601553T3 (da) 2017-03-31 2026-02-16 Danisco Us Inc Alfa-amylase-kombinationsvarianter
CA3067837A1 (en) 2017-06-30 2019-01-03 Danisco Us Inc Low-agglomeration, enzyme-containing particles
EP3668973A2 (en) 2017-08-18 2020-06-24 Danisco US Inc. Alpha-amylase variants
CN107653283B (zh) * 2017-10-31 2021-02-09 新疆浙大阳光生物科技有限公司 一种黄霉素的制备方法
EP3717643A1 (en) 2017-11-29 2020-10-07 Danisco US Inc. Subtilisin variants having improved stability
EP3728543B1 (en) 2017-12-21 2025-10-29 Danisco US Inc. Enzyme-containing, hot-melt granules comprising a thermotolerant desiccant
US20200359656A1 (en) 2018-02-08 2020-11-19 Danisco Us Inc. Thermally-resistant wax matrix particles for enzyme encapsulation
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
US12584119B2 (en) 2018-07-31 2026-03-24 Danisco Us Inc Variant alpha-amylases having amino acid substitutions that lower the PKA of the general acid
EP3844255A1 (en) 2018-08-30 2021-07-07 Danisco US Inc. Enzyme-containing granules
JP2022503923A (ja) 2018-09-27 2022-01-12 ダニスコ・ユーエス・インク 医療用器具を洗浄するための組成物
WO2020077331A2 (en) 2018-10-12 2020-04-16 Danisco Us Inc Alpha-amylases with mutations that improve stability in the presence of chelants
EP3887515A1 (en) 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
CN114174504B (zh) 2019-05-24 2026-04-17 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2020247582A1 (en) 2019-06-06 2020-12-10 Danisco Us Inc Methods and compositions for cleaning
US10941432B1 (en) * 2019-08-15 2021-03-09 Santiago De Urbina Gaviria Method for the preparation of low molecular weight porcine lympho-reticular polypeptides
EP4048683A2 (en) 2019-10-24 2022-08-31 Danisco US Inc Variant maltopentaose/maltohexaose-forming alpha-amylases
US20240034960A1 (en) 2020-08-27 2024-02-01 Danisco Us Inc Enzymes and enzyme compositions for cleaning
CA3189622A1 (en) 2020-08-28 2022-03-03 Kerry Luxembourg S.A. R.L. Feed composition supplemented with a protease combination comprising neutral metalloprotease and serine alkaline protease at a given ratio
JP7510309B2 (ja) * 2020-08-31 2024-07-03 花王株式会社 α-アミラーゼ変異体
EP4284906A1 (en) 2021-01-29 2023-12-06 Danisco US Inc. Compositions for cleaning and methods related thereto
WO2023278297A1 (en) 2021-06-30 2023-01-05 Danisco Us Inc Variant lipases and uses thereof
EP4396320A2 (en) 2021-09-03 2024-07-10 Danisco US Inc. Laundry compositions for cleaning
EP4402258A2 (en) 2021-09-13 2024-07-24 Danisco US Inc. Bioactive-containing granules
EP4448751A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and methods of use
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
EP4448750A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and uses thereof
WO2023114988A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Variant maltopentaose/maltohexaose-forming alpha-amylases
JP2023095355A (ja) * 2021-12-24 2023-07-06 花王株式会社 アミラーゼ配合洗浄剤組成物
WO2023168234A1 (en) 2022-03-01 2023-09-07 Danisco Us Inc. Enzymes and enzyme compositions for cleaning
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法
CN119487167A (zh) 2022-06-21 2025-02-18 丹尼斯科美国公司 用于清洁的包含具有嗜热菌蛋白酶活性的多肽的组合物和方法
EP4581119A1 (en) 2022-09-02 2025-07-09 Danisco US Inc. Detergent compositions and methods related thereto
CN120112635A (zh) 2022-09-02 2025-06-06 丹尼斯科美国公司 枯草杆菌蛋白酶变体及其相关方法
EP4581137A1 (en) 2022-09-02 2025-07-09 Danisco Us Inc Mannanase variants and methods of use
CN120303400A (zh) 2022-11-09 2025-07-11 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2024163584A1 (en) 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
EP4677080A1 (en) 2023-03-06 2026-01-14 Danisco US Inc. Subtilisin variants and methods of use
CN120813242A (zh) 2023-03-16 2025-10-17 营养与生物科学美国第四公司 用于清洁和臭味控制的短芽孢杆菌发酵物提取物及其用途
WO2025071996A1 (en) 2023-09-28 2025-04-03 Danisco Us Inc. Variant cutinase enzymes with improved solubility and uses thereof
WO2025085351A1 (en) 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use
WO2026024921A1 (en) 2024-07-25 2026-01-29 The Procter & Gamble Company Detergent composition comprising a subtilisin variant and methods of use
WO2026050315A1 (en) 2024-08-29 2026-03-05 Danisco Us Inc. Subtilisin variants and methods of use

Family Cites Families (190)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
DE2413561A1 (de) 1974-03-21 1975-10-02 Henkel & Cie Gmbh Lagerbestaendiger, leichtloeslicher waschmittelzusatz und verfahren zu dessen herstellung
US3929678A (en) 1974-08-01 1975-12-30 Procter & Gamble Detergent composition having enhanced particulate soil removal performance
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
NO840200L (no) 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
US4536477A (en) 1983-08-17 1985-08-20 Cpc International Inc. Thermostable glucoamylase and method for its production
US5422267A (en) 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
US4587215A (en) 1984-06-25 1986-05-06 Uop Inc. Highly thermostable amyloglucosidase
DE3428834A1 (de) 1984-08-04 1986-02-13 Henkel KGaA, 4000 Düsseldorf Geschirreinigungsmittel
US4628031A (en) 1984-09-18 1986-12-09 Michigan Biotechnology Institute Thermostable starch converting enzymes
GB8514708D0 (en) 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
GB8514707D0 (en) 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
DK154572C (da) 1985-08-07 1989-04-24 Novo Industri As Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler
JPH0697997B2 (ja) 1985-08-09 1994-12-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ 新規の酵素的洗浄剤添加物
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
JPS63503390A (ja) 1986-05-21 1988-12-08 ノボ インダストリ アクテイ−ゼルスカブ 被覆された洗剤用酵素製品
ATE110768T1 (de) 1986-08-29 1994-09-15 Novo Nordisk As Enzymhaltiger reinigungsmittelzusatz.
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
GB8629536D0 (en) 1986-12-10 1987-01-21 Unilever Plc Enzymatic detergent composition
GB8629534D0 (en) 1986-12-10 1987-01-21 Unilever Plc Enzymatic detergent & bleaching composition
DE3854249T2 (de) 1987-08-28 1996-02-29 Novonordisk As Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen.
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
EP0471265B1 (en) 1988-01-07 1995-10-25 Novo Nordisk A/S Specific protease
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
WO1989009259A1 (en) 1988-03-24 1989-10-05 Novo-Nordisk A/S A cellulase preparation
US5776757A (en) 1988-03-24 1998-07-07 Novo Nordisk A/S Fungal cellulase composition containing alkaline CMC-endoglucanase and essentially no cellobiohydrolase and method of making thereof
GB8811045D0 (en) 1988-05-10 1988-06-15 Unilever Plc Enzymatic detergent composition
GB8813687D0 (en) 1988-06-09 1988-07-13 Unilever Plc Enzymatic dishwashing & rinsing composition
DK78089D0 (da) 1989-02-20 1989-02-20 Novo Industri As Detergentholdigt granulat og fremgangsmaade til fremstilling deraf
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5156773A (en) 1989-12-12 1992-10-20 Novo Nordisk A/S Stabilized enzymatic liquid detergent composition
JP2930719B2 (ja) 1990-03-01 1999-08-03 ノボ ノルディスク アクティーゼルスカブ リパーゼ含有液体プレスポッターおよびその使用
US5447649A (en) 1990-03-01 1995-09-05 Novo Nordisk A/S Lipase containing liquid pre-spotter and use of such pre-spotter
EP0528828B2 (de) 1990-04-14 1997-12-03 Genencor International GmbH Alkalische bacillus-lipasen, hierfür codierende dna-sequenzen sowie bacilli, die diese lipasen produzieren
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
CA2082279C (en) 1990-05-09 2007-09-04 Grethe Rasmussen Cellulase preparation comprising an endoglucanase enzyme
US5162210A (en) 1990-06-29 1992-11-10 Iowa State University Research Foundation Process for enzymatic hydrolysis of starch to glucose
DE69129988T2 (de) 1990-09-13 1999-03-18 Novo Nordisk A/S, Bagsvaerd Lipase-varianten
SK21093A3 (en) 1990-09-28 1993-10-06 Procter & Gamble Polyhydroxy fatty acid amide surfactants to enhace enzyme performance
WO1992010581A1 (en) 1990-12-10 1992-06-25 Genencor International, Inc. IMPROVED SACCHARIFICATION OF CELLULOSE BY CLONING AND AMPLIFICATION OF THE β-GLUCOSIDASE GENE OF TRICHODERMA REESEI
DE69133035T2 (de) 1991-01-16 2003-02-13 The Procter & Gamble Company, Cincinnati Kompakte Waschmittelzusammensetzungen mit hochaktiven Cellulasen
DK0583420T3 (da) 1991-04-30 1996-07-29 Procter & Gamble Builderholdige flydende detergenter med borsyre-polyol-kompleks til inhibering af proteolytisk enzym
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
KR100258460B1 (ko) 1991-05-01 2000-06-01 한센 핀 베네드 안정화 효소 및 세제 조성물
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
DE4232170C2 (de) 1992-09-25 1999-09-16 Henkel Kgaa Schwachalkalische Geschirreinigungsmittel
US5898025A (en) 1992-09-25 1999-04-27 Henkel Kommanditgesellschaft Auf Aktien Mildly alkaline dishwashing detergents
EP1431389A3 (en) 1992-10-06 2004-06-30 Novozymes A/S Cellulase variants
US5281526A (en) 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
DE69333065T2 (de) 1992-12-22 2004-04-01 Novozymes A/S Alkalische lipase
ATE287946T1 (de) 1993-04-27 2005-02-15 Genencor Int Neuartige lipasevarianten zur verwendung in reinigungsmitteln
DK52393D0 (https=) 1993-05-05 1993-05-05 Novo Nordisk As
CA2162021A1 (en) 1993-05-05 1994-11-10 POUL NõRGAARD CHRISTENSEN Cleaning gel
DK76893D0 (https=) 1993-06-28 1993-06-28 Novo Nordisk As
DE4322229A1 (de) 1993-07-05 1995-01-12 Cognis Bio Umwelt Umhüllte Enzymzubereitung für Wasch- und Reinigungsmittel
US5840677A (en) 1993-07-09 1998-11-24 Novo Nordisk A/S Boronic acid or borinic acid derivatives as enzyme stabilizers
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
EP0724631A1 (en) 1993-10-13 1996-08-07 Novo Nordisk A/S H 2?o 2?-stable peroxidase variants
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
DE4344215A1 (de) 1993-12-23 1995-06-29 Cognis Bio Umwelt Silberkorrosionsschutzmittelhaltige Enzymzubereitung
EP0746618B1 (en) 1994-02-22 2002-08-21 Novozymes A/S A method of preparing a variant of a lipolytic enzyme
DE4405511A1 (de) 1994-02-22 1995-08-24 Henkel Kgaa Waschmittel mit amorphen silikatischen Buildersubstanzen
WO1995023221A1 (en) 1994-02-24 1995-08-31 Cognis, Inc. Improved enzymes and detergents containing them
US5691295A (en) 1995-01-17 1997-11-25 Cognis Gesellschaft Fuer Biotechnologie Mbh Detergent compositions
WO1995024471A1 (en) 1994-03-08 1995-09-14 Novo Nordisk A/S Novel alkaline cellulases
JP3851656B2 (ja) 1994-05-04 2006-11-29 ジェネンコア インターナショナル インコーポレーテッド 改善された界面活性剤耐性を有するリパーゼ
WO1995035381A1 (en) 1994-06-20 1995-12-28 Unilever N.V. Modified pseudomonas lipases and their use
WO1996000292A1 (en) 1994-06-23 1996-01-04 Unilever N.V. Modified pseudomonas lipases and their use
DE4422433A1 (de) 1994-06-28 1996-01-04 Cognis Bio Umwelt Mehrenzymgranulat
DE69535733T2 (de) 1994-10-06 2009-04-23 Novozymes A/S Ein enzympräparat mit endoglucanase aktivität
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
BR9509525A (pt) 1994-10-26 1995-10-26 Novo Nordisk As Construção de dna vetor de expressão recombinante célula processo para produzir a enzima que exibe atividade lipolítica enzima que exibe atividade lipolítica preparação de enzima aditivo de detergente e composição de detergente
JPH10507639A (ja) 1994-10-26 1998-07-28 ノボ ノルディスク アクティーゼルスカブ 酵素界面活性剤組成物
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
EP0815209B2 (en) 1995-03-17 2015-02-25 Novozymes A/S Novel endoglucanases
CN1103810C (zh) 1995-06-13 2003-03-26 诺沃奇梅兹有限公司 含苯基硼酸衍生物酶稳定剂的液冻组合物
US6071356A (en) 1995-07-12 2000-06-06 Novo Nordisk Als Cleaning-in-place with a solution containing a protease and a lipase
WO1997007202A1 (en) 1995-08-11 1997-02-27 Novo Nordisk A/S Novel lipolytic enzymes
AU6414196A (en) 1995-07-14 1997-02-18 Novo Nordisk A/S A modified enzyme with lipolytic activity
AU6513096A (en) 1995-07-19 1997-02-18 Novo Nordisk A/S Treatment of fabrics
DE19615776A1 (de) 1996-04-20 1997-10-23 Henkel Kgaa Löslichkeitsverbessertes Enzymgranulat
US5763385A (en) * 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
AU3938997A (en) 1996-08-26 1998-03-19 Novo Nordisk A/S A novel endoglucanase
BR9711479B1 (pt) 1996-09-17 2009-08-11 variante de celulase tendo uma resistência aumentada a tensìdeo de ánion.
CA2265734A1 (en) 1996-10-08 1998-04-16 Novo Nordisk A/S Diaminobenzoic acid derivatives as dye precursors
BR9712473B1 (pt) 1996-11-04 2009-08-11 variantes de subtilase e composições.
US6300116B1 (en) 1996-11-04 2001-10-09 Novozymes A/S Modified protease having improved autoproteolytic stability
DE19703364A1 (de) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
WO1998034946A1 (en) 1997-02-12 1998-08-13 Massachusetts Institute Of Technology Daxx, a novel fas-binding protein that activates jnk and apoptosis
US6060441A (en) 1997-04-10 2000-05-09 Henkel Corporation Cleaning compositions having enhanced enzyme activity
DE19717329A1 (de) 1997-04-24 1998-10-29 Henkel Ecolab Gmbh & Co Ohg Flüssige Enzymzubereitung und ihre Verwendung
WO1999028448A1 (en) 1997-11-26 1999-06-10 Novo Nordisk A/S Thermostable glucoamylase
AU2207099A (en) 1997-12-24 1999-07-19 Genencor International, Inc. An improved method of assaying for a preferred enzyme and/or preferred detergentcomposition
AU3247699A (en) 1998-02-17 1999-09-06 Novo Nordisk A/S Lipase variant
CA2331340A1 (en) 1998-07-15 2000-01-27 Novozymes A/S Glucoamylase variants
US6376450B1 (en) 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
DE19904512A1 (de) 1999-02-04 2000-08-17 Henkel Ecolab Gmbh & Co Ohg Verfahren zur Reinigung von Mehrwegflaschen
US6939702B1 (en) 1999-03-31 2005-09-06 Novozymes A/S Lipase variant
DE19914811A1 (de) 1999-03-31 2000-10-05 Henkel Kgaa Enzym- und bleichaktivatorhaltige Wasch- und Reinigungsmittel
DE60040980D1 (de) 1999-06-02 2009-01-15 Novozymes As Chemisch veränderte, lipolytische enzyme
US6254645B1 (en) 1999-08-20 2001-07-03 Genencor International, Inc. Enzymatic modification of the surface of a polyester fiber or article
US6933140B1 (en) 1999-11-05 2005-08-23 Genencor International, Inc. Enzymes useful for changing the properties of polyester
CA2417547A1 (en) 2000-07-28 2003-01-28 Henkel Kommanditgesellschaft Auf Aktien Novel amylolytic enzyme extracted from bacillus sp. a 7-7 (dsm 12368) and washing and cleaning agents containing this novel amylolytic enzyme
US8283300B2 (en) 2000-11-27 2012-10-09 The Procter & Gamble Company Detergent products, methods and manufacture
EP1516918B2 (en) 2000-11-27 2015-07-01 The Procter & Gamble Company Detergent package
ATE373716T1 (de) 2000-11-28 2007-10-15 Henkel Kgaa Cyclodextrin -glucanotransferase(cg tase) aus bacillus agaradherens(dsm 9948)sowie wasch-und reinigungsmittel mit dieser neuen cyclodextrin- glucanotransferase
DE10121463A1 (de) 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10138753B4 (de) 2001-08-07 2017-07-20 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen
DE10142124A1 (de) 2001-08-30 2003-03-27 Henkel Kgaa Umhüllte Wirkstoffzubereitung für den Einsatz in teilchenförmigen Wasch- und Reinigungsmitteln
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10237200A1 (de) 2002-08-14 2004-03-04 Henkel Kgaa Portionierte Wasch- oder Reinigungsmittelzusammensetzung
US20060035800A1 (en) 2002-12-11 2006-02-16 Novozymes A/S Detergent composition
CN1751116A (zh) 2003-02-18 2006-03-22 诺和酶股份有限公司 洗涤剂组合物
DE10361081A1 (de) 2003-06-13 2005-01-05 Henkel Kgaa Verfahren zur Stabilisierung von Peroxycarbonsäuren in tensidhaltigen Dispersionen
DE10361084A1 (de) 2003-06-13 2005-01-05 Henkel Kgaa Lagerstabile Bleichmittelzusammensetzungen auf Basis von Peroxycarbonsäuren
US8703459B2 (en) 2003-12-05 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Catalytic domains of beta(1,4)-galactosyltransferase I having altered metal ion specificity
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
DE102004019751A1 (de) 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
DE102004048590A1 (de) 2004-04-27 2005-11-24 Henkel Kgaa Reinigungsmittel mit Klarspül-Sulfopolymer und einer speziellen α-Amylase
DE102004048591A1 (de) 2004-04-27 2005-11-24 Henkel Kgaa Reinigungsmittel mit Klarspültensid und einer speziellen α-Amylase
DE102004025859A1 (de) 2004-05-24 2005-12-22 Henkel Kgaa Kit aus Schwamm und Reiniger
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
DE102004040849A1 (de) 2004-08-23 2006-03-02 Henkel Kgaa Klares Wasch- und Reinigungsmittel mit Fließgrenze
WO2006032277A1 (en) 2004-09-21 2006-03-30 Novozymes A/S Subtilases
DE102004047097A1 (de) 2004-09-29 2006-04-06 Henkel Kgaa Wasch- und Reinigungsmittel mit immobilisierten aktiven Inhaltsstoffen
DE102004047777B4 (de) 2004-10-01 2018-05-09 Basf Se Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung
EP1661978B1 (en) 2004-11-29 2011-03-02 The Procter & Gamble Company Detergent compositions
EP1726636B2 (en) 2005-03-03 2016-11-23 The Procter & Gamble Company Detergent Compositions
EP1698689A1 (en) 2005-03-03 2006-09-06 The Procter & Gamble Company Detergent compositions
DE102005015328A1 (de) 2005-04-01 2006-10-05 Henkel Kgaa Klares Wasch- und Reinigungsmittel mit Fließgrenze
GB0522659D0 (en) 2005-11-07 2005-12-14 Reckitt Benckiser Nv Delivery cartridge
WO2007134614A1 (de) 2006-05-18 2007-11-29 Henkel Ag & Co. Kgaa Farbschützendes waschmittel
EP1867707B1 (en) 2006-06-16 2011-09-07 The Procter & Gamble Company Detergent compositions
EP1876226B1 (en) 2006-07-07 2011-03-23 The Procter & Gamble Company Detergent compositions
EP2041253A4 (en) 2006-07-03 2012-06-06 Novozymes Biologicals Inc CLEANING COMPOSITION
ATE502998T1 (de) 2006-07-07 2011-04-15 Procter & Gamble Waschmittelzusammensetzungen
US7968691B2 (en) 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
DE102006039873B4 (de) 2006-08-25 2013-10-31 Henkel Ag & Co. Kgaa Verstärkung der Reinigungsleistung von Waschmitteln durch baumwollaktives schmutzablösevermögendes Cellulosederivat
DE102007006629A1 (de) 2007-02-06 2008-08-07 Henkel Ag & Co. Kgaa Reinigungsmittel
EP2132291A2 (de) 2007-04-03 2009-12-16 Henkel AG & Co. KGaA Waschmittel, enthaltend schmutzablösevermögende wirkstoffe
EP2134826B1 (de) 2007-04-03 2015-11-04 Henkel AG & Co. KGaA Vergrauungsinhibierendes waschmittel
WO2008153805A2 (en) * 2007-05-30 2008-12-18 Danisco Us, Inc., Genencor Division Improved variants of the bacillus licheniformis alpha-amylase
FR2918671B1 (fr) 2007-07-10 2010-10-15 Sanofi Pasteur Milieu de culture d'haemophilus influenzae type b.
DE102007033104A1 (de) 2007-07-13 2009-01-15 Henkel Ag & Co. Kgaa Mittel enthaltend Proteasen aus Stenotrophomonas maltophilia
US9150849B2 (en) * 2007-11-02 2015-10-06 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
EP2215201A2 (en) * 2007-11-05 2010-08-11 Danisco US Inc. Alpha-amylase variants with altered properties
CA2704644C (en) * 2007-11-05 2017-10-03 Danisco Us Inc. Variants of bacillus licheniformis alpha-amylase with increased thermostability and/or decreased calcium dependence
KR20100088675A (ko) 2007-11-05 2010-08-10 다니스코 유에스 인크. 변경된 특성을 지닌 바실러스 종 ts-23 알파-아밀라아제의 변이체
EP3323889B1 (en) 2007-11-20 2020-03-25 Danisco US Inc. Glucoamylase variants with altered properties
US8066818B2 (en) 2008-02-08 2011-11-29 The Procter & Gamble Company Water-soluble pouch
EP2262885B1 (en) 2008-03-28 2013-05-15 Novozymes A/S Triggered release system
US8691743B2 (en) 2008-05-14 2014-04-08 Novozymes A/S Liquid detergent compositions
GB0810881D0 (en) 2008-06-16 2008-07-23 Unilever Plc Improvements relating to fabric cleaning
DE102008038479A1 (de) 2008-08-20 2010-02-25 Henkel Ag & Co. Kgaa Wasch- oder Reinigungsmittel mit gesteigerter Waschkraft
CA2736907C (en) 2008-10-03 2018-06-05 E.I. Du Pont De Nemours And Company Improved perhydrolases for enzymatic peracid generation
US20100125046A1 (en) 2008-11-20 2010-05-20 Denome Frank William Cleaning products
US20110281324A1 (en) 2008-12-01 2011-11-17 Danisco Us Inc. Enzymes With Lipase Activity
WO2010066631A1 (en) 2008-12-12 2010-06-17 Henkel Ag & Co. Kgaa Laundry article having cleaning and conditioning properties
DE102008061859A1 (de) 2008-12-15 2010-06-17 Henkel Ag & Co. Kgaa Maschinelles Geschirrspülmittel
DE102009001186A1 (de) 2009-02-26 2010-09-02 Henkel Ag & Co. Kgaa Handgeschirrspülmittel
DE102009001688A1 (de) 2009-03-20 2010-09-23 Henkel Ag & Co. Kgaa Aldehyd-haltiges Wasch-, Reinigungs-, Nachbehandlungs-oder Waschhilfsmittel mit antibakterieller Wirkung
DK2414514T3 (en) 2009-04-01 2015-08-24 Danisco Us Inc FORMATIONS AND PROCEDURES COMPREHENSIVE Alpha- amylase variants WITH CHANGED PROPERTIES
GB0911428D0 (en) 2009-07-02 2009-08-12 Reckitt Benckiser Nv Composition
DE102009046217A1 (de) 2009-10-30 2011-05-12 Henkel Ag & Co. Kgaa Flüssiges maschinelles Geschirrspülmittel
US8728790B2 (en) 2009-12-09 2014-05-20 Danisco Us Inc. Compositions and methods comprising protease variants
MX2012006616A (es) 2009-12-09 2012-06-21 Procter & Gamble Productos para el cuidado de las telas y el hogar.
EP2343310A1 (en) 2010-01-08 2011-07-13 Novozymes A/S Serine hydrolase formulation
US9896673B2 (en) 2010-02-10 2018-02-20 Novozymes A/S Compositions of high stability alpha amylase variants
EP2357220A1 (en) 2010-02-10 2011-08-17 The Procter & Gamble Company Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent
EP2377914B1 (en) 2010-04-19 2016-11-09 The Procter & Gamble Company Mildly alkaline, low-built, solid fabric treatment detergent composition comprising perhydrolase
US20110240510A1 (en) 2010-04-06 2011-10-06 Johan Maurice Theo De Poortere Optimized release of bleaching systems in laundry detergents
US20120067373A1 (en) 2010-04-15 2012-03-22 Philip Frank Souter Automatic Dishwashing Detergent Composition
WO2011130222A2 (en) 2010-04-15 2011-10-20 Danisco Us Inc. Compositions and methods comprising variant proteases
WO2011131585A1 (en) 2010-04-20 2011-10-27 Henkel Ag & Co. Kgaa Laundry article having cleaning properties
EP3095861B1 (en) 2010-05-06 2019-06-26 The Procter and Gamble Company Consumer products with protease variants
EP2576762A4 (en) 2010-06-03 2013-12-04 Mascoma Corp YEAST FOR THE EXPRESSION OF SACCHAROLYTIC ENZYMES FOR CONSOLIDATED BIOVERIC PROCESSING USING STARCH AND CELLULOSE
EP2395070A1 (en) 2010-06-10 2011-12-14 The Procter & Gamble Company Liquid laundry detergent composition comprising lipase of bacterial origin
EP2395071A1 (en) 2010-06-10 2011-12-14 The Procter & Gamble Company Solid detergent composition comprising lipase of bacterial origin
WO2012145062A1 (en) 2011-02-16 2012-10-26 The Procter & Gamble Company Liquid cleaning compositions
WO2012142087A1 (en) 2011-04-12 2012-10-18 The Procter & Gamble Company Metal bleach catalysts
DE102012201522A1 (de) * 2012-02-02 2013-08-08 Basf Se Lagerstabiles flüssiges Geschirrspülmittel enthaltend Protease und Amylase
EP3354728B1 (en) * 2012-12-21 2020-04-22 Danisco US Inc. Alpha-amylase variants

Also Published As

Publication number Publication date
CN105960456A (zh) 2016-09-21
BR112016010551A2 (pt) 2017-12-05
EP3071691B1 (en) 2019-10-23
WO2015077126A1 (en) 2015-05-28
US20160272957A1 (en) 2016-09-22
MX2016006489A (es) 2016-08-03
JP2016538860A (ja) 2016-12-15
EP3071691A1 (en) 2016-09-28

Similar Documents

Publication Publication Date Title
JP6560214B2 (ja) プロテアーゼ開裂に対する感受性を低減した変異体α−アミラーゼ、及びその使用方法
CN105229147B (zh) α-淀粉酶组合变体
EP2859097B1 (en) Alpha-amylase variants derived from the alpha amylase of cytophaga sp. amylasei(cspamy2).
JP2015518707A (ja) 糖化のための、aspergillusclavatus由来アルファアミラーゼの使用
CN103917642A (zh) 变体成麦芽六糖α-淀粉酶变体
JP6499081B2 (ja) アスペルギルス・フミガタス(Aspergillusfumigatus)由来のグルコアミラーゼを発現するトリコデルマ・レーシ(Trichodermareesei)宿主細胞、及びその使用方法
CN104884614A (zh) α-淀粉酶变体
JP2019500058A (ja) α−アミラーゼ組み合わせ変異体
EP3060659B1 (en) Alpha-amylases from exiguobacterium, and methods of use, thereof
JP2016501512A (ja) 糖化のためにタラロミセス・エメルソニ(TALAROMYCESEMERSONII)由来のα−アミラーゼを使用する方法
CN104903461A (zh) 将支链淀粉酶和来自土曲霉的α-淀粉酶用于糖化的方法
CN104870631A (zh) 表达来自烟曲霉的葡糖淀粉酶的里氏木霉宿主细胞和其使用方法
JP2015534456A (ja) アスペルギルス・クラバタス(Aspergillusclavatus)由来のαアミラーゼ及びイソアミラーゼを糖化に使用する方法
EP3052622B1 (en) Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof
CN104903459A (zh) 将支链淀粉酶和来自烟曲霉的α-淀粉酶用于糖化的方法
WO2015021600A1 (en) Beta-amylase and methods of use
US20180112203A1 (en) Amylase with maltogenic properties
WO2014200658A1 (en) Alpha-amylase from promicromonospora vindobonensis
CN104903460A (zh) 将异淀粉酶和来自土曲霉的α-淀粉酶用于糖化的方法
WO2014200656A1 (en) Alpha-amylase from streptomyces umbrinus
EP3011020A1 (en) Alpha-amylase from bacillaceae family member
CN104822829A (zh) 具有产麦芽糖特性的淀粉酶

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171026

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20171026

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20180815

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180828

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20181127

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190128

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20190625

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20190718

R150 Certificate of patent or registration of utility model

Ref document number: 6560214

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

LAPS Cancellation because of no payment of annual fees