CN105960456A - 对蛋白酶裂解敏感性降低的变体α-淀粉酶及其使用方法 - Google Patents

对蛋白酶裂解敏感性降低的变体α-淀粉酶及其使用方法 Download PDF

Info

Publication number
CN105960456A
CN105960456A CN201480063251.2A CN201480063251A CN105960456A CN 105960456 A CN105960456 A CN 105960456A CN 201480063251 A CN201480063251 A CN 201480063251A CN 105960456 A CN105960456 A CN 105960456A
Authority
CN
China
Prior art keywords
amylase
alpha
amino acid
starch
variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480063251.2A
Other languages
English (en)
Chinese (zh)
Inventor
D·芬南
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Publication of CN105960456A publication Critical patent/CN105960456A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201480063251.2A 2013-11-20 2014-11-13 对蛋白酶裂解敏感性降低的变体α-淀粉酶及其使用方法 Pending CN105960456A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361906617P 2013-11-20 2013-11-20
US61/906,617 2013-11-20
PCT/US2014/065522 WO2015077126A1 (en) 2013-11-20 2014-11-13 Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof

Publications (1)

Publication Number Publication Date
CN105960456A true CN105960456A (zh) 2016-09-21

Family

ID=52023630

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480063251.2A Pending CN105960456A (zh) 2013-11-20 2014-11-13 对蛋白酶裂解敏感性降低的变体α-淀粉酶及其使用方法

Country Status (7)

Country Link
US (1) US20160272957A1 (https=)
EP (1) EP3071691B1 (https=)
JP (1) JP6560214B2 (https=)
CN (1) CN105960456A (https=)
BR (1) BR112016010551A2 (https=)
MX (1) MX2016006489A (https=)
WO (1) WO2015077126A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113166745A (zh) * 2018-10-12 2021-07-23 丹尼斯科美国公司 在螯合剂存在下具有可增强稳定性的突变的α-淀粉酶

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104884614A (zh) * 2012-12-21 2015-09-02 丹尼斯科美国公司 α-淀粉酶变体
US20180030456A1 (en) * 2015-02-19 2018-02-01 Danisco Us Inc. Enhanced protein expression
CN107835855B (zh) 2015-05-13 2022-05-13 丹尼斯科美国公司 AprL-进化枝蛋白酶变体及其用途
FI3307427T3 (fi) 2015-06-09 2023-11-09 Danisco Us Inc Osmoottiset puhkeavat kapselit
WO2016201040A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc. Water-triggered enzyme suspension
WO2016201069A1 (en) 2015-06-09 2016-12-15 Danisco Us Inc Low-density enzyme-containing particles
US11499146B2 (en) 2015-06-17 2022-11-15 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
JP7364330B2 (ja) 2015-11-05 2023-10-18 ダニスコ・ユーエス・インク パエニバチルス（Ｐａｅｎｉｂａｃｉｌｌｕｓ）属種及びバチルス（Ｂａｃｉｌｌｕｓ）属種のマンナナーゼ
EP4141113A1 (en) 2015-11-05 2023-03-01 Danisco US Inc Paenibacillus sp. mannanases
US11920170B2 (en) 2015-12-09 2024-03-05 Danisco Us Inc. Alpha-amylase combinatorial variants
EP3390625B1 (en) 2015-12-18 2023-09-06 Danisco US Inc. Polypeptides with endoglucanase activity and uses thereof
DE102016201643A1 (de) * 2016-02-03 2017-08-03 Henkel Ag & Co. Kgaa Verbesserte Waschleistung durch eine alpha-Amylase aus Bacillus cereus
JP2019518440A (ja) 2016-05-03 2019-07-04 ダニスコ・ユーエス・インク プロテアーゼ変異体およびその使用
CN109072213A (zh) 2016-05-05 2018-12-21 丹尼斯科美国公司 蛋白酶变体及其用途
CN109312319B (zh) * 2016-05-09 2023-05-16 诺维信公司 具有改善的性能的变体多肽及其用途
WO2017210295A1 (en) 2016-05-31 2017-12-07 Danisco Us Inc. Protease variants and uses thereof
EP3472313B1 (en) 2016-06-17 2022-08-31 Danisco US Inc. Protease variants and uses thereof
EP3535365A2 (en) 2016-11-07 2019-09-11 Danisco US Inc. Laundry detergent composition
CN110312795B (zh) 2016-12-21 2024-07-23 丹尼斯科美国公司 蛋白酶变体及其用途
EP3559226B1 (en) 2016-12-21 2023-01-04 Danisco US Inc. Bacillus gibsonii-clade serine proteases
US11453871B2 (en) 2017-03-15 2022-09-27 Danisco Us Inc. Trypsin-like serine proteases and uses thereof
EP3601553B1 (en) 2017-03-31 2025-12-03 Danisco US Inc. Alpha-amylase combinatorial variants
EP3601515A1 (en) 2017-03-31 2020-02-05 Danisco US Inc. Delayed release enzyme formulations for bleach-containing detergents
MX2019014556A (es) 2017-06-30 2020-02-07 Danisco Us Inc Particulas que contienen enzimas de baja aglomeracion.
EP3668973A2 (en) 2017-08-18 2020-06-24 Danisco US Inc. Alpha-amylase variants
CN107653283B (zh) * 2017-10-31 2021-02-09 新疆浙大阳光生物科技有限公司 一种黄霉素的制备方法
US20200354708A1 (en) 2017-11-29 2020-11-12 Danisco Us Inc. Subtilisin variants having improved stability
MX2020006518A (es) 2017-12-21 2020-10-28 Danisco Us Inc Gránulos de fusión en caliente, que contienen enzimas, que comprenden un desecante termotolerante.
US20200359656A1 (en) 2018-02-08 2020-11-19 Danisco Us Inc. Thermally-resistant wax matrix particles for enzyme encapsulation
WO2019245704A1 (en) 2018-06-19 2019-12-26 Danisco Us Inc Subtilisin variants
EP3799601A1 (en) 2018-06-19 2021-04-07 Danisco US Inc. Subtilisin variants
EP3830231A1 (en) 2018-07-31 2021-06-09 Danisco US Inc. Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid
WO2020047215A1 (en) 2018-08-30 2020-03-05 Danisco Us Inc Enzyme-containing granules
WO2020068486A1 (en) 2018-09-27 2020-04-02 Danisco Us Inc Compositions for medical instrument cleaning
US12509672B2 (en) 2018-11-28 2025-12-30 Danisco Us Inc. Subtilisin variants having improved stability
US20220220419A1 (en) 2019-05-24 2022-07-14 Danisco Us Inc Subtilisin variants and methods of use
US20220306968A1 (en) 2019-06-06 2022-09-29 Danisco Us Inc Methods and compositions for cleaning
US10941432B1 (en) * 2019-08-15 2021-03-09 Santiago De Urbina Gaviria Method for the preparation of low molecular weight porcine lympho-reticular polypeptides
BR112022007697A2 (pt) 2019-10-24 2022-07-12 Danisco Us Inc Alfa-amilase variante que forma maltopentaose/maltohexaose
EP4204553A1 (en) 2020-08-27 2023-07-05 Danisco US Inc. Enzymes and enzyme compositions for cleaning
CA3189622A1 (en) 2020-08-28 2022-03-03 Kerry Luxembourg S.A. R.L. Feed composition supplemented with a protease combination comprising neutral metalloprotease and serine alkaline protease at a given ratio
JP7510309B2 (ja) * 2020-08-31 2024-07-03 花王株式会社 α－アミラーゼ変異体
EP4284906A1 (en) 2021-01-29 2023-12-06 Danisco US Inc. Compositions for cleaning and methods related thereto
EP4363565A1 (en) 2021-06-30 2024-05-08 Danisco US Inc. Variant lipases and uses thereof
US20240384205A1 (en) 2021-09-03 2024-11-21 Danisco Us Inc. Laundry compositions for cleaning
CN117957318A (zh) 2021-09-13 2024-04-30 丹尼斯科美国公司 含有生物活性物质的颗粒
CN118679251A (zh) 2021-12-16 2024-09-20 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2023114939A2 (en) 2021-12-16 2023-06-22 Danisco Us Inc. Subtilisin variants and methods of use
EP4448747A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Variant maltopentaose/maltohexaose-forming alpha-amylases
EP4448750A2 (en) 2021-12-16 2024-10-23 Danisco US Inc. Subtilisin variants and uses thereof
JP2023095355A (ja) * 2021-12-24 2023-07-06 花王株式会社 アミラーゼ配合洗浄剤組成物
CN118974227A (zh) 2022-03-01 2024-11-15 丹尼斯科美国公司 用于清洁的酶和酶组合物
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2023250301A1 (en) 2022-06-21 2023-12-28 Danisco Us Inc. Methods and compositions for cleaning comprising a polypeptide having thermolysin activity
WO2024050339A1 (en) 2022-09-02 2024-03-07 Danisco Us Inc. Mannanase variants and methods of use
CN120112635A (zh) 2022-09-02 2025-06-06 丹尼斯科美国公司 枯草杆菌蛋白酶变体及其相关方法
EP4581119A1 (en) 2022-09-02 2025-07-09 Danisco US Inc. Detergent compositions and methods related thereto
EP4615968A1 (en) 2022-11-09 2025-09-17 Danisco US Inc. Subtilisin variants and methods of use
WO2024163584A1 (en) 2023-02-01 2024-08-08 Danisco Us Inc. Subtilisin variants and methods of use
CN120712348A (zh) 2023-03-06 2025-09-26 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
EP4680013A1 (en) 2023-03-16 2026-01-21 Nutrition & Biosciences USA 4, Inc. Brevibacillus fermentate extracts for cleaning and malodor control and use thereof
CN121909283A (zh) 2023-09-28 2026-04-21 丹尼斯科美国公司 具有改善的溶解度的变体角质酶及其用途
CN121969733A (zh) 2023-10-20 2026-05-01 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2026024921A1 (en) 2024-07-25 2026-01-29 The Procter & Gamble Company Detergent composition comprising a subtilisin variant and methods of use
WO2026050315A1 (en) 2024-08-29 2026-03-05 Danisco Us Inc. Subtilisin variants and methods of use

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101848986A (zh) * 2007-11-05 2010-09-29 丹尼斯科美国公司 具有改变的特性的α-淀粉酶变体
CN101878303A (zh) * 2007-11-02 2010-11-03 斯克利普斯研究院 使用包含非天然氨基酸的蛋白质进行定向进化
CN101883841A (zh) * 2007-11-05 2010-11-10 丹尼斯科美国公司 具有增强的热稳定性和/或减少的钙依赖性的地衣芽孢杆菌α-淀粉酶的变体

Family Cites Families (187)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
GB1483591A (en) 1973-07-23 1977-08-24 Novo Industri As Process for coating water soluble or water dispersible particles by means of the fluid bed technique
DE2413561A1 (de) 1974-03-21 1975-10-02 Henkel & Cie Gmbh Lagerbestaendiger, leichtloeslicher waschmittelzusatz und verfahren zu dessen herstellung
DE2437090A1 (de) 1974-08-01 1976-02-19 Hoechst Ag Reinigungsmittel
GB1590432A (en) 1976-07-07 1981-06-03 Novo Industri As Process for the production of an enzyme granulate and the enzyme granuate thus produced
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
NO840200L (no) 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
US4536477A (en) 1983-08-17 1985-08-20 Cpc International Inc. Thermostable glucoamylase and method for its production
US5422267A (en) 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
US4587215A (en) 1984-06-25 1986-05-06 Uop Inc. Highly thermostable amyloglucosidase
DE3428834A1 (de) 1984-08-04 1986-02-13 Henkel KGaA, 4000 Düsseldorf Geschirreinigungsmittel
US4628031A (en) 1984-09-18 1986-12-09 Michigan Biotechnology Institute Thermostable starch converting enzymes
GB8514708D0 (en) 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
GB8514707D0 (en) 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
DK154572C (da) 1985-08-07 1989-04-24 Novo Industri As Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler
JPH0697997B2 (ja) 1985-08-09 1994-12-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ 新規の酵素的洗浄剤添加物
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
EP0270608B1 (en) 1986-05-21 1990-08-22 Novo Nordisk A/S Coated detergent enzymes
DE3750450T2 (de) 1986-08-29 1995-01-05 Novo Industri As Enzymhaltiger Reinigungsmittelzusatz.
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
GB8629534D0 (en) 1986-12-10 1987-01-21 Unilever Plc Enzymatic detergent & bleaching composition
GB8629536D0 (en) 1986-12-10 1987-01-21 Unilever Plc Enzymatic detergent composition
EP0305216B1 (en) 1987-08-28 1995-08-02 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
DE68924654T2 (de) 1988-01-07 1996-04-04 Novonordisk As Spezifische Protease.
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
JP2728531B2 (ja) 1988-03-24 1998-03-18 ノボ ノルディスク アクティーゼルスカブ セルラーゼ調製品
US5648263A (en) 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
GB8811045D0 (en) 1988-05-10 1988-06-15 Unilever Plc Enzymatic detergent composition
GB8813687D0 (en) 1988-06-09 1988-07-13 Unilever Plc Enzymatic dishwashing & rinsing composition
DK78089D0 (da) 1989-02-20 1989-02-20 Novo Industri As Detergentholdigt granulat og fremgangsmaade til fremstilling deraf
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5156773A (en) 1989-12-12 1992-10-20 Novo Nordisk A/S Stabilized enzymatic liquid detergent composition
US5447649A (en) 1990-03-01 1995-09-05 Novo Nordisk A/S Lipase containing liquid pre-spotter and use of such pre-spotter
WO1991013142A1 (en) 1990-03-01 1991-09-05 Novo Nordisk A/S Lipase containing liquid pre-spotter and use of such pre-spotter
KR100236540B1 (ko) 1990-04-14 2000-01-15 레클로우크스 라우에르 알카리성 바실러스-리파제, 이를 코-딩하는 dna 서열 및 리파제를 생산하는 바실러스 균주
DK0531372T4 (da) 1990-05-09 2004-08-09 Novozymes As Cellulasepræparat omfattende et endoglucanaseenzym
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
US5162210A (en) 1990-06-29 1992-11-10 Iowa State University Research Foundation Process for enzymatic hydrolysis of starch to glucose
AU657278B2 (en) 1990-09-13 1995-03-09 Novo Nordisk A/S Lipase variants
CZ37393A3 (en) 1990-09-28 1994-04-13 Procter & Gamble Liquid cleansing preparation with enhanced stability and cleansing efficiency of enzyme
ES2322032T3 (es) 1990-12-10 2009-06-16 Genencor Int Sacrificacion mejorada de celulosa por clonacion y amplificacion del gen de beta-glucosidasa de trichoderma reesei.
ES2174820T3 (es) 1991-01-16 2002-11-16 Procter & Gamble Composiciones detergentes compactas con celulasa de alta actividad.
CZ285148B6 (cs) 1991-04-30 1999-05-12 The Procter And Gamble Company Kapalné detergentní směsi s borito-polyolovým komplexem k inhibici proteolytického enzymu
EP0511456A1 (en) 1991-04-30 1992-11-04 The Procter & Gamble Company Liquid detergents with aromatic borate ester to inhibit proteolytic enzyme
ES2121014T3 (es) 1991-05-01 1998-11-16 Novo Nordisk As Enzimas estabilizadas y composiciones detergentes.
DK72992D0 (da) 1992-06-01 1992-06-01 Novo Nordisk As Enzym
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
DE4232170C2 (de) 1992-09-25 1999-09-16 Henkel Kgaa Schwachalkalische Geschirreinigungsmittel
US5898025A (en) 1992-09-25 1999-04-27 Henkel Kommanditgesellschaft Auf Aktien Mildly alkaline dishwashing detergents
ATE262035T1 (de) 1992-10-06 2004-04-15 Novozymes As Zellulosevarianten
US5281526A (en) 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
JP3507076B2 (ja) 1992-12-22 2004-03-15 ノボザイムス アクティーゼルスカブ アルカリリパーゼ
KR950702240A (ko) 1993-04-27 1995-06-19 한스 발터 라벤 세제로의 이용을 위한 새로운 리파제 변형체
EP0697037A1 (en) 1993-05-05 1996-02-21 Novo Nordisk A/S Cleaning gel
DK52393D0 (https=) 1993-05-05 1993-05-05 Novo Nordisk As
DK76893D0 (https=) 1993-06-28 1993-06-28 Novo Nordisk As
DE4322229A1 (de) 1993-07-05 1995-01-12 Cognis Bio Umwelt Umhüllte Enzymzubereitung für Wasch- und Reinigungsmittel
WO1995002046A1 (en) 1993-07-09 1995-01-19 Novo Nordisk A/S Boronic acid or borinic acid derivatives as enzyme stabilizers
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
US5817495A (en) 1993-10-13 1998-10-06 Novo Nordisk A/S H2 O2 -stable peroxidase variants
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
DE4344215A1 (de) 1993-12-23 1995-06-29 Cognis Bio Umwelt Silberkorrosionsschutzmittelhaltige Enzymzubereitung
DE4405511A1 (de) 1994-02-22 1995-08-24 Henkel Kgaa Waschmittel mit amorphen silikatischen Buildersubstanzen
DE69527835T2 (de) 1994-02-22 2003-04-10 Novozymes A/S, Bagsvaerd Methode zur herstellung einer variante eines lipolytischen enzymes
EP1921147B1 (en) 1994-02-24 2011-06-08 Henkel AG & Co. KGaA Improved enzymes and detergents containing them
US5691295A (en) 1995-01-17 1997-11-25 Cognis Gesellschaft Fuer Biotechnologie Mbh Detergent compositions
DE69536145D1 (de) 1994-03-08 2011-04-07 Novozymes As Neuartige alkalische Zellulasen
WO1995030744A2 (en) 1994-05-04 1995-11-16 Genencor International Inc. Lipases with improved surfactant resistance
WO1995035381A1 (en) 1994-06-20 1995-12-28 Unilever N.V. Modified pseudomonas lipases and their use
AU2884695A (en) 1994-06-23 1996-01-19 Unilever Plc Modified pseudomonas lipases and their use
DE4422433A1 (de) 1994-06-28 1996-01-04 Cognis Bio Umwelt Mehrenzymgranulat
DE69535733T2 (de) 1994-10-06 2009-04-23 Novozymes A/S Ein enzympräparat mit endoglucanase aktivität
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
AU3741995A (en) 1994-10-26 1996-05-23 Novo Nordisk A/S Enzymatic detergent composition
US5827719A (en) 1994-10-26 1998-10-27 Novo Nordisk A/S Enzyme with lipolytic activity
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
CN102080070B (zh) 1995-03-17 2016-01-20 诺沃奇梅兹有限公司 新的内切葡聚糖酶
DE69621131T2 (de) 1995-06-13 2002-11-28 Novozymes A/S, Bagsvaerd 4-substituierte-phenylboronsäuren als enzymstabilisatoren
US6071356A (en) 1995-07-12 2000-06-06 Novo Nordisk Als Cleaning-in-place with a solution containing a protease and a lipase
EP0839186B1 (en) 1995-07-14 2004-11-10 Novozymes A/S A modified enzyme with lipolytic activity
EP0839224A1 (en) 1995-07-19 1998-05-06 Novo Nordisk A/S Treatment of fabrics
AU6655196A (en) 1995-08-11 1997-03-12 Novo Nordisk A/S Novel lipolytic enzymes
DE19615776A1 (de) 1996-04-20 1997-10-23 Henkel Kgaa Löslichkeitsverbessertes Enzymgranulat
US5763385A (en) * 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
AU3938997A (en) 1996-08-26 1998-03-19 Novo Nordisk A/S A novel endoglucanase
EP1726644A1 (en) 1996-09-17 2006-11-29 Novozymes A/S Cellulase variants
WO1998015257A1 (en) 1996-10-08 1998-04-16 Novo Nordisk A/S Diaminobenzoic acid derivatives as dye precursors
EP0932667B1 (en) 1996-11-04 2008-10-01 Novozymes A/S Subtilase variants and compositions
JP4044143B2 (ja) 1996-11-04 2008-02-06 ノボザイムス アクティーゼルスカブ ズブチラーゼ変異体及び組成物
DE19703364A1 (de) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
WO1998034946A1 (en) 1997-02-12 1998-08-13 Massachusetts Institute Of Technology Daxx, a novel fas-binding protein that activates jnk and apoptosis
US6060441A (en) 1997-04-10 2000-05-09 Henkel Corporation Cleaning compositions having enhanced enzyme activity
DE19717329A1 (de) 1997-04-24 1998-10-29 Henkel Ecolab Gmbh & Co Ohg Flüssige Enzymzubereitung und ihre Verwendung
CN1261567C (zh) 1997-11-26 2006-06-28 诺维信公司 热稳定的葡糖淀粉酶
DE69837559T3 (de) 1997-12-24 2011-07-21 Genencor International, Inc., Calif. Verfahren zur Bestimmung der Waschleistung eines Enzyms.
AU3247699A (en) 1998-02-17 1999-09-06 Novo Nordisk A/S Lipase variant
MXPA01000352A (es) 1998-07-15 2002-06-04 Novozymes As Varientes de glucoamilasa.
US6376450B1 (en) 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
DE19904512A1 (de) 1999-02-04 2000-08-17 Henkel Ecolab Gmbh & Co Ohg Verfahren zur Reinigung von Mehrwegflaschen
AU3420100A (en) 1999-03-31 2000-10-23 Novozymes A/S Lipase variant
DE19914811A1 (de) 1999-03-31 2000-10-05 Henkel Kgaa Enzym- und bleichaktivatorhaltige Wasch- und Reinigungsmittel
JP4851034B2 (ja) 1999-06-02 2012-01-11 ノボザイムス アクティーゼルスカブ 化学修飾された脂質分解酵素
US6254645B1 (en) 1999-08-20 2001-07-03 Genencor International, Inc. Enzymatic modification of the surface of a polyester fiber or article
US6933140B1 (en) 1999-11-05 2005-08-23 Genencor International, Inc. Enzymes useful for changing the properties of polyester
CN100491525C (zh) 2000-07-28 2009-05-27 汉高两合股份公司 从芽孢杆菌a7-7(dsm 12368)中提取的新型淀粉分解酶以及含有该新型淀粉分解酶的洗涤剂和清洗剂
CA2426645C (en) 2000-11-27 2008-06-10 The Procter & Gamble Company Detergent pouch comprising superposed or superposable compartments
US8283300B2 (en) 2000-11-27 2012-10-09 The Procter & Gamble Company Detergent products, methods and manufacture
PL361363A1 (en) 2000-11-28 2004-10-04 Henkel Kommanditgesellschaft Auf Aktien Novel cyclodextrin glucanotransferase (cgtase), obtained from bacillus agaradherens (dsm 9948) and detergents and cleaning agents containing said novel cyclodextrin glucanotransferase
DE10121463A1 (de) 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10138753B4 (de) 2001-08-07 2017-07-20 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel mit Hybrid-Alpha-Amylasen
DE10142124A1 (de) 2001-08-30 2003-03-27 Henkel Kgaa Umhüllte Wirkstoffzubereitung für den Einsatz in teilchenförmigen Wasch- und Reinigungsmitteln
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10237200A1 (de) 2002-08-14 2004-03-04 Henkel Kgaa Portionierte Wasch- oder Reinigungsmittelzusammensetzung
MXPA05006071A (es) 2002-12-11 2005-09-30 Novozymes As Composicion detergente.
WO2004074419A2 (en) 2003-02-18 2004-09-02 Novozymes A/S Detergent compositions
DE10361084A1 (de) 2003-06-13 2005-01-05 Henkel Kgaa Lagerstabile Bleichmittelzusammensetzungen auf Basis von Peroxycarbonsäuren
DE10361081A1 (de) 2003-06-13 2005-01-05 Henkel Kgaa Verfahren zur Stabilisierung von Peroxycarbonsäuren in tensidhaltigen Dispersionen
US8703459B2 (en) 2003-12-05 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Catalytic domains of beta(1,4)-galactosyltransferase I having altered metal ion specificity
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
DE102004019751A1 (de) 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
DE102004048590A1 (de) 2004-04-27 2005-11-24 Henkel Kgaa Reinigungsmittel mit Klarspül-Sulfopolymer und einer speziellen α-Amylase
DE102004048591A1 (de) 2004-04-27 2005-11-24 Henkel Kgaa Reinigungsmittel mit Klarspültensid und einer speziellen α-Amylase
DE102004025859A1 (de) 2004-05-24 2005-12-22 Henkel Kgaa Kit aus Schwamm und Reiniger
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
DE102004040849A1 (de) 2004-08-23 2006-03-02 Henkel Kgaa Klares Wasch- und Reinigungsmittel mit Fließgrenze
ATE533843T1 (de) 2004-09-21 2011-12-15 Novozymes As Subtilasen
DE102004047097A1 (de) 2004-09-29 2006-04-06 Henkel Kgaa Wasch- und Reinigungsmittel mit immobilisierten aktiven Inhaltsstoffen
DE102004047777B4 (de) 2004-10-01 2018-05-09 Basf Se Alpha-Amylase-Varianten mit erhöhter Lösungsmittelstabilität, Verfahren zu deren Herstellung sowie deren Verwendung
EP1661978B1 (en) 2004-11-29 2011-03-02 The Procter & Gamble Company Detergent compositions
EP1726636B2 (en) 2005-03-03 2016-11-23 The Procter & Gamble Company Detergent Compositions
EP1698689A1 (en) 2005-03-03 2006-09-06 The Procter & Gamble Company Detergent compositions
DE102005015328A1 (de) 2005-04-01 2006-10-05 Henkel Kgaa Klares Wasch- und Reinigungsmittel mit Fließgrenze
GB0522659D0 (en) 2005-11-07 2005-12-14 Reckitt Benckiser Nv Delivery cartridge
EP2021451A1 (de) 2006-05-18 2009-02-11 Henkel AG & Co. KGaA Farbschützendes waschmittel
EP1867707B1 (en) 2006-06-16 2011-09-07 The Procter & Gamble Company Detergent compositions
US20080023031A1 (en) 2006-07-03 2008-01-31 Novozymes Biologicals, Inc. Cleaning Composition
ATE503011T1 (de) 2006-07-07 2011-04-15 Procter & Gamble Waschmittelzusammensetzungen
ATE502998T1 (de) 2006-07-07 2011-04-15 Procter & Gamble Waschmittelzusammensetzungen
US7968691B2 (en) 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
DE102006039873B4 (de) 2006-08-25 2013-10-31 Henkel Ag & Co. Kgaa Verstärkung der Reinigungsleistung von Waschmitteln durch baumwollaktives schmutzablösevermögendes Cellulosederivat
DE102007006629A1 (de) 2007-02-06 2008-08-07 Henkel Ag & Co. Kgaa Reinigungsmittel
WO2008119836A2 (de) 2007-04-03 2008-10-09 Henkel Ag & Co. Kgaa Waschmittel, enthaltend schmutzablösevermögende wirkstoffe
ES2554983T3 (es) 2007-04-03 2015-12-28 Henkel Ag & Co. Kgaa Agente de lavado inhibidor del agrisado
EP2155870A2 (en) * 2007-05-30 2010-02-24 Danisco US, INC., Genencor Division Improved variants of the bacillus licheniformis alpha-amylase
FR2918671B1 (fr) 2007-07-10 2010-10-15 Sanofi Pasteur Milieu de culture d'haemophilus influenzae type b.
DE102007033104A1 (de) 2007-07-13 2009-01-15 Henkel Ag & Co. Kgaa Mittel enthaltend Proteasen aus Stenotrophomonas maltophilia
KR20100088675A (ko) 2007-11-05 2010-08-10 다니스코 유에스 인크. 변경된 특성을 지닌 바실러스 종 ｔｓ-23 알파-아밀라아제의 변이체
ES2527586T3 (es) 2007-11-20 2015-01-27 Danisco Us Inc. Variantes de glucoamilasa con propiedades modificadas
US8066818B2 (en) 2008-02-08 2011-11-29 The Procter & Gamble Company Water-soluble pouch
US10557108B2 (en) 2008-03-28 2020-02-11 Novozymes A/S Triggered release system
EP2285944B1 (en) 2008-05-14 2013-03-13 Novozymes A/S Liquid detergent compositions
GB0810881D0 (en) 2008-06-16 2008-07-23 Unilever Plc Improvements relating to fabric cleaning
DE102008038479A1 (de) 2008-08-20 2010-02-25 Henkel Ag & Co. Kgaa Wasch- oder Reinigungsmittel mit gesteigerter Waschkraft
AU2009298420B2 (en) 2008-10-03 2016-05-05 E. I. Du Pont De Nemours And Company Improved perhydrolases for enzymatic peracid generation
US20100125046A1 (en) 2008-11-20 2010-05-20 Denome Frank William Cleaning products
EP2367923A2 (en) 2008-12-01 2011-09-28 Danisco US Inc. Enzymes with lipase activity
WO2010066631A1 (en) 2008-12-12 2010-06-17 Henkel Ag & Co. Kgaa Laundry article having cleaning and conditioning properties
DE102008061859A1 (de) 2008-12-15 2010-06-17 Henkel Ag & Co. Kgaa Maschinelles Geschirrspülmittel
DE102009001186A1 (de) 2009-02-26 2010-09-02 Henkel Ag & Co. Kgaa Handgeschirrspülmittel
DE102009001688A1 (de) 2009-03-20 2010-09-23 Henkel Ag & Co. Kgaa Aldehyd-haltiges Wasch-, Reinigungs-, Nachbehandlungs-oder Waschhilfsmittel mit antibakterieller Wirkung
EP2414515A2 (en) 2009-04-01 2012-02-08 Danisco US Inc. Cleaning system comprising an alpha-amylase and a protease
GB0911428D0 (en) 2009-07-02 2009-08-12 Reckitt Benckiser Nv Composition
DE102009046217A1 (de) 2009-10-30 2011-05-12 Henkel Ag & Co. Kgaa Flüssiges maschinelles Geschirrspülmittel
AR079338A1 (es) 2009-12-09 2012-01-18 Danisco Us Inc Variantes de proteasa de bacillus y acidos nucleicos que codifican dichas variantes
CN102652175B (zh) 2009-12-09 2016-02-10 宝洁公司 织物和家居护理产品
EP2343310A1 (en) 2010-01-08 2011-07-13 Novozymes A/S Serine hydrolase formulation
EP3404087A1 (en) 2010-02-10 2018-11-21 Novozymes A/S Alpha-amylase variants with high stability in presence of a chelating agent
EP2357220A1 (en) 2010-02-10 2011-08-17 The Procter & Gamble Company Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent
EP2377914B1 (en) 2010-04-19 2016-11-09 The Procter & Gamble Company Mildly alkaline, low-built, solid fabric treatment detergent composition comprising perhydrolase
US20110240510A1 (en) 2010-04-06 2011-10-06 Johan Maurice Theo De Poortere Optimized release of bleaching systems in laundry detergents
PL2558573T3 (pl) 2010-04-15 2017-08-31 Danisco Us Inc. Kompozycje i sposoby obejmujące warianty proteazy
US20120067373A1 (en) 2010-04-15 2012-03-22 Philip Frank Souter Automatic Dishwashing Detergent Composition
WO2011131585A1 (en) 2010-04-20 2011-10-27 Henkel Ag & Co. Kgaa Laundry article having cleaning properties
JP6448904B2 (ja) 2010-05-06 2019-01-09 ダニスコ・ユーエス・インク サブチリシン変異体を含む組成物及び方法
WO2011153516A2 (en) 2010-06-03 2011-12-08 Mascoma Corporation Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose
EP2395070A1 (en) 2010-06-10 2011-12-14 The Procter & Gamble Company Liquid laundry detergent composition comprising lipase of bacterial origin
EP2395071A1 (en) 2010-06-10 2011-12-14 The Procter & Gamble Company Solid detergent composition comprising lipase of bacterial origin
US8846596B2 (en) 2011-02-16 2014-09-30 The Procter & Gamble Company Liquid cleaning compositions
WO2012142087A1 (en) 2011-04-12 2012-10-18 The Procter & Gamble Company Metal bleach catalysts
DE102012201522A1 (de) * 2012-02-02 2013-08-08 Basf Se Lagerstabiles flüssiges Geschirrspülmittel enthaltend Protease und Amylase
CN104884614A (zh) * 2012-12-21 2015-09-02 丹尼斯科美国公司 α-淀粉酶变体

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101878303A (zh) * 2007-11-02 2010-11-03 斯克利普斯研究院 使用包含非天然氨基酸的蛋白质进行定向进化
CN101848986A (zh) * 2007-11-05 2010-09-29 丹尼斯科美国公司 具有改变的特性的α-淀粉酶变体
CN101883841A (zh) * 2007-11-05 2010-11-10 丹尼斯科美国公司 具有增强的热稳定性和/或减少的钙依赖性的地衣芽孢杆菌α-淀粉酶的变体
CN101932703A (zh) * 2007-11-05 2010-12-29 丹尼斯科美国公司 具有改变的特性的α-淀粉酶变体

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
N.S.REDDY等: "An overview of the microbial α-amylase family.", 《AFRICAN JOURNAL OF BIOTECHNOLOGY》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113166745A (zh) * 2018-10-12 2021-07-23 丹尼斯科美国公司 在螯合剂存在下具有可增强稳定性的突变的α-淀粉酶

Also Published As

Publication number Publication date
EP3071691A1 (en) 2016-09-28
JP2016538860A (ja) 2016-12-15
US20160272957A1 (en) 2016-09-22
MX2016006489A (es) 2016-08-03
JP6560214B2 (ja) 2019-08-14
EP3071691B1 (en) 2019-10-23
BR112016010551A2 (pt) 2017-12-05
WO2015077126A1 (en) 2015-05-28

Similar Documents

Publication Publication Date Title
EP3071691B1 (en) Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof
CN105229147B (zh) α-淀粉酶组合变体
CN104379738A (zh) 衍生自噬纤维菌属物种(CYTOPHAGA SP.)淀粉酶|(CSPAMY2)的α淀粉酶的α淀粉酶变体
CN103917642A (zh) 变体成麦芽六糖α-淀粉酶变体
CN104884614A (zh) α-淀粉酶变体
WO2013169645A1 (en) Use of alpha-amylase from aspergillus clavatus for saccharification
EP3060659B1 (en) Alpha-amylases from exiguobacterium, and methods of use, thereof
CN104903461A (zh) 将支链淀粉酶和来自土曲霉的α-淀粉酶用于糖化的方法
WO2014092960A1 (en) Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof
CA2886578A1 (en) Method of using alpha-amylase from talaromyces emersonii for saccharification
CN104583412A (zh) 采用棒曲霉α-淀粉酶和支链淀粉酶由淀粉制备葡萄糖的方法
CN104640994A (zh) 将异淀粉酶和来自棒曲霉的α-淀粉酶用于糖化的方法
WO2017173324A2 (en) Alpha-amylases, compositions & methods
EP3052622B1 (en) Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof
CN104769106A (zh) 使用来自埃默森篮状菌(TALAROMYCES EMERSONII)的α-淀粉酶进行糖化的方法
WO2015021600A1 (en) Beta-amylase and methods of use
WO2017173190A2 (en) Alpha-amylases, compositions & methods
US20180112203A1 (en) Amylase with maltogenic properties
WO2014200658A1 (en) Alpha-amylase from promicromonospora vindobonensis
WO2014200656A1 (en) Alpha-amylase from streptomyces umbrinus
CN104903460A (zh) 将异淀粉酶和来自土曲霉的α-淀粉酶用于糖化的方法
US20160130571A1 (en) Alpha-Amylase from Bacillaceae Family Member
WO2014200657A1 (en) Alpha-amylase from streptomyces xiamenensis
CN104822829A (zh) 具有产麦芽糖特性的淀粉酶
WO2015094809A1 (en) Chimeric fungal alpha-amylases comprising carbohydrate binding module and the use thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160921