JP5889910B2 - 新規セレンテラジン基質及び使用法 - Google Patents

新規セレンテラジン基質及び使用法 Download PDF

Info

Publication number
JP5889910B2
JP5889910B2 JP2013537799A JP2013537799A JP5889910B2 JP 5889910 B2 JP5889910 B2 JP 5889910B2 JP 2013537799 A JP2013537799 A JP 2013537799A JP 2013537799 A JP2013537799 A JP 2013537799A JP 5889910 B2 JP5889910 B2 JP 5889910B2
Authority
JP
Japan
Prior art keywords
pbi
coelenterazine
ogluc
luminescence
luciferase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2013537799A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014503485A (ja
Inventor
ディーター エイチ クラウバート
ディーター エイチ クラウバート
ポンチョ マイゼンハイマー
ポンチョ マイゼンハイマー
ジェームズ アンク
ジェームズ アンク
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44983728&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP5889910(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Promega Corp filed Critical Promega Corp
Publication of JP2014503485A publication Critical patent/JP2014503485A/ja
Application granted granted Critical
Publication of JP5889910B2 publication Critical patent/JP5889910B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12013—Oplophorus-luciferin 2-monooxygenase (1.13.12.13)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
JP2013537799A 2010-11-02 2011-11-02 新規セレンテラジン基質及び使用法 Active JP5889910B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40942210P 2010-11-02 2010-11-02
US61/409,422 2010-11-02
PCT/US2011/059017 WO2012061529A1 (en) 2010-11-02 2011-11-02 Novel coelenterazine substrates and methods of use

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016028303A Division JP6574721B2 (ja) 2010-11-02 2016-02-17 新規セレンテラジン基質及び使用法

Publications (2)

Publication Number Publication Date
JP2014503485A JP2014503485A (ja) 2014-02-13
JP5889910B2 true JP5889910B2 (ja) 2016-03-22

Family

ID=44983728

Family Applications (6)

Application Number Title Priority Date Filing Date
JP2013537799A Active JP5889910B2 (ja) 2010-11-02 2011-11-02 新規セレンテラジン基質及び使用法
JP2013537800A Active JP6155424B2 (ja) 2010-11-02 2011-11-02 オキヒオドシエビ属由来ルシフェラーゼ、新規セレンテラジン基質、及び使用法
JP2016024952A Active JP6374420B2 (ja) 2010-11-02 2016-02-12 オキヒオドシエビ属由来ルシフェラーゼ、新規セレンテラジン基質、及び使用法
JP2016028303A Active JP6574721B2 (ja) 2010-11-02 2016-02-17 新規セレンテラジン基質及び使用法
JP2017084390A Active JP6539689B2 (ja) 2010-11-02 2017-04-21 オキヒオドシエビ属由来ルシフェラーゼ、新規セレンテラジン基質、及び使用法
JP2018040100A Active JP6752839B2 (ja) 2010-11-02 2018-03-06 新規セレンテラジン基質及び使用法

Family Applications After (5)

Application Number Title Priority Date Filing Date
JP2013537800A Active JP6155424B2 (ja) 2010-11-02 2011-11-02 オキヒオドシエビ属由来ルシフェラーゼ、新規セレンテラジン基質、及び使用法
JP2016024952A Active JP6374420B2 (ja) 2010-11-02 2016-02-12 オキヒオドシエビ属由来ルシフェラーゼ、新規セレンテラジン基質、及び使用法
JP2016028303A Active JP6574721B2 (ja) 2010-11-02 2016-02-17 新規セレンテラジン基質及び使用法
JP2017084390A Active JP6539689B2 (ja) 2010-11-02 2017-04-21 オキヒオドシエビ属由来ルシフェラーゼ、新規セレンテラジン基質、及び使用法
JP2018040100A Active JP6752839B2 (ja) 2010-11-02 2018-03-06 新規セレンテラジン基質及び使用法

Country Status (15)

Country Link
US (11) US8809529B2 (enExample)
EP (4) EP3498710A1 (enExample)
JP (6) JP5889910B2 (enExample)
KR (2) KR101939553B1 (enExample)
CN (3) CN103443121B (enExample)
AU (2) AU2011323418B2 (enExample)
BR (2) BR112013010487B1 (enExample)
CA (3) CA3081115A1 (enExample)
DK (2) DK2635595T3 (enExample)
ES (2) ES2567410T3 (enExample)
IL (5) IL225867B (enExample)
PL (3) PL2635595T4 (enExample)
PT (1) PT2635595T (enExample)
SG (5) SG10201608550SA (enExample)
WO (2) WO2012061529A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018127459A (ja) * 2010-11-02 2018-08-16 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 新規セレンテラジン基質及び使用法

Families Citing this family (88)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2541765A1 (en) 2003-10-10 2004-10-01 Promega Corporation Luciferase biosensor
EP2327768B1 (en) 2006-04-03 2015-09-09 Promega Corporation Permuted and nonpermuted luciferase biosensors
WO2009142735A2 (en) 2008-05-19 2009-11-26 Promega Corporation LUCIFERASE BIOSENSORS FOR cAMP
LT3409764T (lt) * 2009-05-01 2020-06-10 Promega Corporation Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga
US9290794B2 (en) * 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US8735559B2 (en) 2010-05-11 2014-05-27 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
WO2012061477A1 (en) 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
EP2782916B1 (en) 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
AU2013359160B2 (en) 2012-12-12 2018-11-15 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
AU2013359166B2 (en) 2012-12-12 2019-09-19 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
JP6511720B2 (ja) * 2013-02-28 2019-05-15 Jnc株式会社 コドン最適化変異エビルシフェラーゼ遺伝子およびその使用方法
EP2969435B1 (en) 2013-03-15 2021-11-03 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
SG11201507306VA (en) 2013-03-15 2015-10-29 Promega Corp Activation of bioluminescence by structural complementation
US10168323B2 (en) 2013-03-15 2019-01-01 Promega Corporation Compositions and methods for capture of cellular targets of bioactive agents
JP2016517687A (ja) * 2013-03-27 2016-06-20 サンプル テクノロジーズ，インコーポレイティド 組換えファージ及び細菌検出方法
JP6040846B2 (ja) * 2013-04-08 2016-12-07 Jnc株式会社 セレンテラジン類縁体
AU2014281406A1 (en) 2013-06-19 2016-01-21 Sample6 Technologies, Inc. Phage-based bacterial detection assay
HK1222673A1 (zh) 2013-07-12 2017-07-07 The Children's Hospital Of Philadelphia Aav载体和用於抗aav(腺相关病毒)中和抗体的检测
EP3022316B1 (en) 2013-07-18 2017-11-29 Ecole Polytechnique Fédérale de Lausanne (EPFL) Means and methods for bioluminescence resonance energy transfer (bret) analysis in a biological sample
JP6484987B2 (ja) 2013-10-21 2019-03-20 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
WO2015067302A1 (en) 2013-11-05 2015-05-14 Ecole Polytechnique Federale De Lausanne (Epfl) Sensor molecules and uses thereof
JP6442825B2 (ja) 2013-12-26 2018-12-26 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
US9790537B2 (en) 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
US9927430B2 (en) 2014-01-29 2018-03-27 Promega Corporation Pro-substrates for live cell applications
JP6256118B2 (ja) 2014-03-11 2018-01-10 Jnc株式会社 エビルシフェラーゼの触媒蛋白質のキメラ遺伝子及びその使用法
JP6265020B2 (ja) 2014-04-16 2018-01-24 Jnc株式会社 エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法
US9676997B2 (en) 2014-05-23 2017-06-13 Promega Corporation Compositions and methods for stabilizing luminogenic substrates
US9732373B2 (en) 2014-09-11 2017-08-15 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US11365402B2 (en) * 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
EP4219736A1 (en) * 2014-09-12 2023-08-02 Promega Corporation Internal protein tags
WO2016098078A2 (en) * 2014-12-19 2016-06-23 Novartis Ag Dimerization switches and uses thereof
EP3254113B1 (en) 2015-02-05 2024-10-09 Promega Corporation Luciferase-based thermal shift assays
JP6876002B2 (ja) 2015-06-05 2021-05-26 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 機能的要素を共有結合により係留させるための細胞透過性、細胞適合性、かつ開裂可能であるリンカー
CN107922428B (zh) 2015-06-25 2021-02-05 普洛麦格公司 噻吩并吡咯化合物及其作为刺虾源性荧光素酶的抑制剂的用途
US20170029798A1 (en) * 2015-07-27 2017-02-02 Cellivery Therapeutics, Inc. Development of Improved Cell-Permeable (iCP) Parkin Recombinant Protein as a Protein-Based Anti-Neurodegenerative Agent for the Treatment of Parkinson's Disease-Associated Phenotypes by Utilizing BBB-Penetrating Protein Delivery System MITT, Enabled by Advanced Macromolecule Transduction Domain (aMTD)
EP3416968B1 (en) * 2016-02-15 2022-07-27 Promega Corporation Coelenterazine analogues
WO2017155036A1 (ja) 2016-03-11 2017-09-14 国立研究開発法人産業技術総合研究所 発光酵素タンパク質
US10634680B2 (en) 2016-04-26 2020-04-28 University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods
CN105968114B (zh) * 2016-05-27 2018-11-09 山东大学 一种腔肠素类似物及其制备方法与应用
JP7212612B2 (ja) * 2016-07-28 2023-01-25 プロメガ コーポレイション セレンテラジン類縁体
US10202584B2 (en) * 2016-09-01 2019-02-12 The Regents Of The University Of California Red-shifted luciferase-luciferin pairs for enhanced bioluminescence
WO2018049241A1 (en) 2016-09-09 2018-03-15 Promega Corporation Dual protected pro-coelenterazine substrates
EP3535270B3 (en) 2016-11-01 2024-05-29 Promega Corporation Coelenterazine analogues tethered to energy acceptors
JP7104702B2 (ja) * 2016-12-01 2022-07-21 プロメガ コーポレイション ５，５－二置換ルシフェリン及びルシフェラーゼ系アッセイにおけるそれらの使用
US10577370B2 (en) 2016-12-01 2020-03-03 Promega Corporation Cell impermeable coelenterazine analogues
US10815247B2 (en) 2016-12-28 2020-10-27 Promega Corporation Functionalized NANOLUC inhibitors
EP3395803A1 (en) 2017-04-28 2018-10-31 Institut Pasteur Imidazopyrazine derivatives, process for preparation thereof, and their uses as luciferins
WO2018219953A1 (en) 2017-05-30 2018-12-06 Ecole polytechnique fédérale de Lausanne (EPFL) Sensors, methods and kits for detecting nadph based on resonance energy transfer.
ES2983923T3 (es) 2017-07-06 2024-10-28 Promega Corp Análogos de la pro-coelenterazina estables en suero
CN108107209B (zh) * 2017-09-19 2020-07-24 中国中医科学院医学实验中心 一种检测分泌型报告蛋白含量的优化方法及其所用生物材料
JP7419260B2 (ja) 2018-05-01 2024-01-22 プロメガ コーポレイション ＮａｎｏＬｕｃ自殺基質としてのセレンテラジン化合物
EP3803401A4 (en) 2018-05-30 2022-02-23 Promega Corporation BROAD SPECTRUM KINASE BINDERS
EP4140991B1 (en) 2018-06-01 2024-05-15 Promega Corporation Inhibitors of oplophorus luciferase-derived bioluminescent complexes
EP3807419A4 (en) * 2018-06-12 2022-09-07 Promega Corporation MULTIPLE LUCIFERASE
US10962538B2 (en) 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
WO2020023871A1 (en) 2018-07-27 2020-01-30 Promega Corporation Quinone-containing conjugates
JP7653351B2 (ja) 2018-08-23 2025-03-28 プロメガ コーポレイション セレンテラジン類似体
CN112996923B (zh) 2018-10-03 2025-02-18 普洛麦格公司 用于稳定腔肠素及其类似物和衍生物的组合物和方法
WO2020113036A2 (en) 2018-11-28 2020-06-04 Promega Corporation Reactive peptide labeling
US20200200765A1 (en) 2018-12-04 2020-06-25 Promega Corporation Broad spectrum gpcr binding agents
WO2020176755A1 (en) * 2019-02-27 2020-09-03 University Of Virginia Patent Foundation Atp-independent bioluminescent reporter variants to improve in vivo imaging
EP3941195A4 (en) 2019-03-20 2023-03-22 Promega Corporation PHOTOAFFINITY PROBES
US11360096B2 (en) 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
CN114450326B (zh) 2019-06-24 2025-02-11 普罗美加公司 用于将生物分子递送到细胞中的改性聚胺聚合物
KR20220121816A (ko) * 2019-11-27 2022-09-01 프로메가 코포레이션 다분할 루시퍼라제 펩타이드 및 폴리펩타이드
US12061202B2 (en) 2019-12-10 2024-08-13 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
JP7790724B2 (ja) * 2020-03-17 2025-12-23 国立研究開発法人産業技術総合研究所 発光基質化合物
EP3882632A1 (en) 2020-03-18 2021-09-22 Universite De Bordeaux Novel ion conducting channel fusion subunits and methods of use thereof
CN113527231A (zh) * 2020-04-15 2021-10-22 深圳华大生命科学研究院 杂环取代腔肠素类化合物的合成方法
EP3904508A1 (en) 2020-04-27 2021-11-03 Institut Pasteur Luciferase linked immunosorbent assay
JP7677997B2 (ja) 2020-05-22 2025-05-15 プロメガ コーポレイション キナーゼターゲットエンゲージメントの増強
CN116391129A (zh) 2020-08-28 2023-07-04 普罗美加公司 针对ras蛋白的靶标接合测定法
KR20230124938A (ko) 2020-12-25 2023-08-28 추가이 세이야쿠 가부시키가이샤 복수의 표적 분자와 함께 복합체를 형성할 수 있는후보 분자의 스크리닝 방법
CN115161296B (zh) * 2021-04-01 2024-08-13 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
EP4337646A4 (en) 2021-05-13 2025-05-21 Promega Corporation BIOLUMINESCENT DETECTION OF DNA SYNTHESIS
AU2022333242A1 (en) * 2021-08-24 2024-03-07 Centre National De La Recherche Scientifique Luminescent based antigen assay
JP2025515181A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 伸長表面ループ領域を有する修飾デハロゲナーゼ
EP4519426A2 (en) 2022-05-04 2025-03-12 Promega Corporation Split modified dehalogenase variants
WO2023215497A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
US20240060059A1 (en) 2022-05-04 2024-02-22 Promega Corporation Circularly permuted dehalogenase variants
EP4549570A4 (en) * 2022-06-30 2026-04-29 Dgi Tech Qing Dao Co Ltd Luciferase Mutant and Its Use
CN115349611B (zh) * 2022-08-05 2023-10-24 江南大学 一种改善南美白对虾虾糜凝胶品质的方法
CN116068198B (zh) * 2022-11-30 2024-01-09 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用
US20240377404A1 (en) * 2023-03-24 2024-11-14 Promega Corporation Bioluminescence-triggered photocatalytic activation
JP7482563B1 (ja) 2023-06-21 2024-05-14 国立大学法人大阪大学 シイノトモシビタケ由来の発光関連遺伝子
DE102023120682B3 (de) 2023-08-03 2024-10-24 Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut Zytotoxizitätstest zum Nachweis von Zellschäden
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3477549D1 (en) 1983-08-17 1989-05-11 Allied Signal Inc Gas generator and method of generating gas
JPH0515398A (ja) 1991-07-15 1993-01-26 Nippon Oil & Fats Co Ltd オキシダーゼ酵素の定量方法
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
EP1978091A1 (en) 1994-01-03 2008-10-08 Promega Corporation Mutant luciferases
US6387675B1 (en) 1994-01-03 2002-05-14 Promega Corporation Mutant luciferases
GB9501170D0 (en) 1994-03-23 1995-03-08 Secr Defence Luciferases
JP3648763B2 (ja) 1994-08-23 2005-05-18 日本油脂株式会社 ウミホタルルシフェリン誘導体および糖加水分解酵素の定量方法
GB9417593D0 (en) 1994-09-01 1994-10-19 Secr Defence Luciferase labelling method
US5670356A (en) 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
DE69633171T2 (de) 1995-01-20 2005-08-18 The Secretary Of State For Defence, Salisbury Mutierte luziferasen
JPH08294397A (ja) 1995-04-27 1996-11-12 Nippon Oil & Fats Co Ltd 免疫学的活性物質の測定方法
US5998204A (en) 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
GB9707486D0 (en) 1997-04-11 1997-05-28 Secr Defence Enzyme assays
US6429286B1 (en) 1997-04-16 2002-08-06 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Immunoregulatory molecules for costimulatory signal transduction
CA2301828A1 (en) 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
JP3694181B2 (ja) * 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法
NZ518413A (en) * 1999-10-26 2004-11-26 Secr Defence Novel enzyme
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
US20010046687A1 (en) 2000-03-31 2001-11-29 Dicesare Joseph L. Method for the enhancement of luminescence intensity from a reaction between adenosine triphosphate (ATP) and a luciferase/luciferin reactant system
EP1156103B1 (en) 2000-04-26 2010-08-11 Chisso Corporation Oplophorus luciferase
JP4613441B2 (ja) * 2000-04-26 2011-01-19 チッソ株式会社 新規ルシフェラーゼおよび発光蛋白質
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US7268229B2 (en) * 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
EP1472238B1 (en) 2002-02-01 2012-07-04 Promega Corporation Bioluminescent protease assay
WO2004027378A2 (en) 2002-09-20 2004-04-01 Promega Corporation Luminescence-based methods and probes for measuring cytochrome p450 activity
DK1413584T3 (da) 2002-10-21 2005-07-04 Axxam S R L Fotoprotein med forbedret bioluminescens
JP4485470B2 (ja) 2002-12-23 2010-06-23 プロメガ コーポレイション ルシフェラーゼ酵素活性を保護するための方法及びキット
EP2341134B1 (en) 2003-01-31 2014-08-27 Promega Corporation Covalent tethering of functional groups to proteins
US7429472B2 (en) 2003-01-31 2008-09-30 Promega Corporation Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
AU2004210982B2 (en) 2003-02-12 2008-12-18 Promega Corporation Methods and kits for dual enzymatic assays whereby light is quenched from luminescent reactions
US20040178545A1 (en) 2003-03-14 2004-09-16 Cates Larry E. System for optically analyzing a molten metal bath
CA2541765A1 (en) 2003-10-10 2004-10-01 Promega Corporation Luciferase biosensor
ATE443070T1 (de) 2004-01-08 2009-10-15 Univ Zuerich Metallkomplexe mit vitamin b12 als ligand
US7553632B2 (en) 2004-01-22 2009-06-30 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7416854B2 (en) 2004-01-22 2008-08-26 Promega Corporation Luminogenic and nonluminogenic multiplex assay
US7425436B2 (en) 2004-07-30 2008-09-16 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US20070087400A1 (en) 2004-07-30 2007-04-19 Aldis Darzins Covalent tethering of functional groups to proteins and substrates therefor
EP3179252B1 (en) 2004-07-30 2018-11-14 Promega Corporation Covalent tethering of functional groups to proteins and substrates therefor
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
JP4639904B2 (ja) 2005-03-30 2011-02-23 チッソ株式会社 蛍光活性を有するルシフェラーゼの活性増強方法
JP3844082B2 (ja) * 2005-04-08 2006-11-08 キッコーマン株式会社 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び耐熱性ホタルルシフェラーゼの製造法
EP2778234B1 (en) 2005-05-31 2017-09-27 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
EP1935986B1 (en) 2005-05-31 2014-04-16 Promega Corporation Luminogenic and fluorogenic compounds and methods to detect molecules or conditions
US7495579B2 (en) 2005-06-13 2009-02-24 Sirota J Marcos Traffic light status remote sensor system
KR20080039431A (ko) 2005-08-17 2008-05-07 예다 리서치 앤드 디벨럽먼트 캄파니 리미티드 생물학적으로 활성인 혈청 파라옥소나제의 수준을 구하기위한 방법 및 조성물
JP4999341B2 (ja) 2005-09-06 2012-08-15 株式会社バイオエネックス 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
EP2327768B1 (en) 2006-04-03 2015-09-09 Promega Corporation Permuted and nonpermuted luciferase biosensors
JP2007306871A (ja) 2006-05-19 2007-11-29 Chisso Corp 封入体可溶化方法及び精製方法
EP2087107A2 (en) 2006-10-30 2009-08-12 Promega Corporation Mutant hydrolase proteins with enhanced kinetics and functional expression
WO2008086035A2 (en) 2007-01-10 2008-07-17 Promega Corporation Split mutant hydrolase fusion reporter and uses thereof
US20080248511A1 (en) 2007-03-26 2008-10-09 Promega Corporation Methods to quench light from optical reactions
EP2215227A2 (en) 2007-11-05 2010-08-11 Promega Corporation Hybrid fusion reporter and uses thereof
WO2009142735A2 (en) 2008-05-19 2009-11-26 Promega Corporation LUCIFERASE BIOSENSORS FOR cAMP
EP2326953B1 (en) 2008-07-22 2018-03-21 Promega Corporation Adp detection based luminescent phosphotransferase or atp hydrolase assay
TWI375901B (en) 2008-08-05 2012-11-01 Elan Microelectronics Corp Touch screen and method for positioning coordinate
GB2479847B (en) * 2009-02-09 2014-02-19 Jnc Corp Coelenterazine analogs and manufacturing method thereof
WO2010119721A1 (ja) 2009-04-17 2010-10-21 独立行政法人産業技術総合研究所 超高輝度で安定な人工生物発光酵素
LT3409764T (lt) * 2009-05-01 2020-06-10 Promega Corporation Sintetinės oplophorus liuciferazės su sustiprinta šviesos išeiga
WO2011038219A1 (en) 2009-09-24 2011-03-31 Promega Corporation Proteases having improved stability, solubility and activity
JP5605727B2 (ja) * 2010-04-06 2014-10-15 Ｊｎｃ株式会社 セレンテラジン類縁体及びセレンテラミド類縁体
US8735559B2 (en) 2010-05-11 2014-05-27 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
WO2012030960A1 (en) 2010-09-01 2012-03-08 Promega Corporation Oxidized glutathione assay
US9284188B2 (en) 2010-09-03 2016-03-15 The Regents Of The University Of California Cytosolic delivery of materials with endosome-disrupting colloids
BR112013010487B1 (pt) * 2010-11-02 2021-02-02 Promega Corporation compostos de coelenterazina, kit compreendendo ditos compostos e método para detectar luminescência em uma amostra in vitro
WO2012061477A1 (en) * 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
JP6067019B2 (ja) 2011-09-02 2017-01-25 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 代謝的に活性な細胞の酸化還元状態を評価するための化合物及び方法、ならびにｎａｄ（ｐ）／ｎａｄ（ｐ）ｈを測定するための方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018127459A (ja) * 2010-11-02 2018-08-16 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 新規セレンテラジン基質及び使用法

Also Published As

Publication number Publication date
WO2012061530A8 (en) 2013-06-06
JP2014503485A (ja) 2014-02-13
KR101939553B1 (ko) 2019-01-17
AU2016213854B2 (en) 2018-01-04
US20200399676A1 (en) 2020-12-24
US12435354B2 (en) 2025-10-07
CN103370319B (zh) 2016-08-24
CN106148362A (zh) 2016-11-23
EP2635582B1 (en) 2016-01-27
WO2012061530A2 (en) 2012-05-10
US20240158831A1 (en) 2024-05-16
SG10201607774RA (en) 2016-11-29
ES2723773T3 (es) 2019-09-02
US9840730B2 (en) 2017-12-12
AU2016213854A1 (en) 2016-09-01
PT2635595T (pt) 2019-01-29
KR20130099997A (ko) 2013-09-06
JP6374420B2 (ja) 2018-08-15
CN103443121A (zh) 2013-12-11
IL274316B (en) 2021-12-01
IL288070A (en) 2022-01-01
IL258790B (en) 2020-05-31
US20140223590A1 (en) 2014-08-07
IL274316A (en) 2020-06-30
EP2635595A2 (en) 2013-09-11
EP3498709A1 (en) 2019-06-19
CN103443121B (zh) 2016-04-20
CN106148362B (zh) 2021-04-30
US20150064731A1 (en) 2015-03-05
JP2014500011A (ja) 2014-01-09
CA3081115A1 (en) 2012-05-10
WO2012061529A1 (en) 2012-05-10
KR20130084312A (ko) 2013-07-24
JP6155424B2 (ja) 2017-07-05
IL288070B (en) 2022-10-01
EP3498709B1 (en) 2025-12-31
KR101538624B1 (ko) 2015-07-22
IL225867A0 (en) 2013-07-31
ES2567410T3 (es) 2016-04-22
PL2635595T3 (pl) 2019-04-30
IL225867B (en) 2018-07-31
AU2011323418B2 (en) 2017-07-27
CA2815227A1 (en) 2012-05-10
CA2815227C (en) 2019-03-26
BR112013010487B1 (pt) 2021-02-02
US12215375B2 (en) 2025-02-04
US20260028662A1 (en) 2026-01-29
EP3498710A1 (en) 2019-06-19
PL3498709T3 (pl) 2026-03-23
PL2635582T3 (pl) 2016-08-31
IL288070B2 (en) 2023-02-01
WO2012061530A3 (en) 2012-09-27
SG189496A1 (en) 2013-05-31
US9951373B2 (en) 2018-04-24
US9938564B2 (en) 2018-04-10
DK2635582T3 (da) 2016-03-14
EP3498709C0 (en) 2025-12-31
JP6752839B2 (ja) 2020-09-09
AU2011323418A1 (en) 2013-05-30
US10774364B2 (en) 2020-09-15
IL258790A (en) 2018-06-28
JP6539689B2 (ja) 2019-07-03
CA2817102A1 (en) 2012-05-10
JP6574721B2 (ja) 2019-09-11
BR112013010855A2 (pt) 2017-06-27
DK2635595T3 (en) 2019-03-04
IL226114B (en) 2018-04-30
JP2017176182A (ja) 2017-10-05
US11661623B2 (en) 2023-05-30
EP2635582A1 (en) 2013-09-11
CN103370319A (zh) 2013-10-23
SG10201608550SA (en) 2016-12-29
JP2018127459A (ja) 2018-08-16
PL2635595T4 (pl) 2019-04-30
US8669103B2 (en) 2014-03-11
AU2011323419A1 (en) 2013-05-30
US20120117667A1 (en) 2012-05-10
US20160002703A1 (en) 2016-01-07
US9139836B2 (en) 2015-09-22
IL226114A0 (en) 2013-06-27
US20180327810A1 (en) 2018-11-15
JP2016144454A (ja) 2016-08-12
CA2817102C (en) 2020-07-28
US8809529B2 (en) 2014-08-19
SG10202103336SA (en) 2021-04-29
JP2016145219A (ja) 2016-08-12
EP2635595B1 (en) 2019-01-09
SG190104A1 (en) 2013-06-28
BR112013010487A2 (pt) 2016-11-22
US20220348983A1 (en) 2022-11-03
US20140227759A1 (en) 2014-08-14
US20120174242A1 (en) 2012-07-05

Similar Documents

Publication Publication Date Title
JP6574721B2 (ja) 新規セレンテラジン基質及び使用法
AU2011323419B2 (en) Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20141031

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150715

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20150716

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20151008

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20151113

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151215

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20160118

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20160217

R150 Certificate of patent or registration of utility model

Ref document number: 5889910

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250