ATE374820T1 - Mutantenluciferase - Google Patents
MutantenluciferaseInfo
- Publication number
- ATE374820T1 ATE374820T1 AT00971589T AT00971589T ATE374820T1 AT E374820 T1 ATE374820 T1 AT E374820T1 AT 00971589 T AT00971589 T AT 00971589T AT 00971589 T AT00971589 T AT 00971589T AT E374820 T1 ATE374820 T1 AT E374820T1
- Authority
- AT
- Austria
- Prior art keywords
- luciferase
- amino acid
- compared
- wild
- type
- Prior art date
Links
- 108060001084 Luciferase Proteins 0.000 title abstract 9
- 239000005089 Luciferase Substances 0.000 title abstract 7
- 150000001413 amino acids Chemical class 0.000 abstract 3
- 230000002378 acidificating effect Effects 0.000 abstract 2
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 abstract 1
- 241000254064 Photinus pyralis Species 0.000 abstract 1
- 101000622060 Photinus pyralis Luciferin 4-monooxygenase Proteins 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000005516 coenzyme A Substances 0.000 abstract 1
- 229940093530 coenzyme a Drugs 0.000 abstract 1
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9925161.3A GB9925161D0 (en) | 1999-10-26 | 1999-10-26 | Novel enzyme |
| GBGB0016744.5A GB0016744D0 (en) | 1999-10-26 | 2000-07-10 | Novel enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE374820T1 true ATE374820T1 (de) | 2007-10-15 |
Family
ID=26244619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00971589T ATE374820T1 (de) | 1999-10-26 | 2000-10-26 | Mutantenluciferase |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP1224294B1 (de) |
| JP (1) | JP5154724B2 (de) |
| CN (1) | CN100516223C (de) |
| AT (1) | ATE374820T1 (de) |
| AU (1) | AU772485C (de) |
| CA (1) | CA2387691A1 (de) |
| DE (1) | DE60036633T2 (de) |
| DK (1) | DK1224294T3 (de) |
| ES (1) | ES2292479T3 (de) |
| NZ (1) | NZ518413A (de) |
| RU (1) | RU2251571C2 (de) |
| WO (1) | WO2001031028A2 (de) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6602677B1 (en) | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
| GB9823468D0 (en) * | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
| GB9925161D0 (en) | 1999-10-26 | 1999-12-22 | Secr Defence | Novel enzyme |
| GB0111275D0 (en) | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| GB2400659B8 (en) | 2003-04-17 | 2007-07-09 | Cambrex Bio Science Nottingham | Assay methods and materials |
| WO2005035719A2 (en) * | 2003-10-03 | 2005-04-21 | Board Of Control Of Michigan Technological University | Modified luciferase |
| GB0517005D0 (en) | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| JP4999341B2 (ja) * | 2005-09-06 | 2012-08-15 | 株式会社バイオエネックス | 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法 |
| WO2007144990A1 (ja) * | 2006-06-12 | 2007-12-21 | National Institute Of Advanced Industrial Science And Technology | 変異型ルシフェラーゼ |
| JP5188771B2 (ja) | 2007-09-26 | 2013-04-24 | 株式会社日立製作所 | 変異型ホタルルシフェラーゼ |
| US8183007B2 (en) | 2008-07-22 | 2012-05-22 | Promega Corporation | ADP detection based methods using adenylate cyclase and bioluminescence |
| JP2011120559A (ja) * | 2008-12-26 | 2011-06-23 | Kikkoman Corp | ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法 |
| DK3181687T3 (en) | 2009-05-01 | 2018-09-10 | Promega Corp | Synthetic oplophorus luciferases with increased light yield |
| US8980603B2 (en) | 2009-06-30 | 2015-03-17 | The General Hospital Corporation | Gaussia luciferase variant for high-throughput screening |
| DK2635583T3 (en) | 2010-11-02 | 2015-08-03 | Promega Corp | Coelenterazinderivater and methods of use thereof |
| EP3498709A1 (de) | 2010-11-02 | 2019-06-19 | Promega Corporation | Aus oplophorus gewonnene luciferasen, neuartige coelenterazinsubstrate und verfahren zur verwendung |
| JP5896679B2 (ja) * | 2011-03-15 | 2016-03-30 | オリンパス株式会社 | オオオバボタル由来ルシフェラーゼ |
| RU2495929C1 (ru) * | 2012-05-30 | 2013-10-20 | ФЕДЕРАЛЬНОЕ ГОСУДАРСТВЕННОЕ БЮДЖЕТНОЕ УЧРЕЖДЕНИЕ НАУКИ Институт биофизики Сибирского отделения Российской академии наук | УКОРОЧЕННАЯ МУТАНТНАЯ ЛЮЦИФЕРАЗА ИЗ Metridia longa ДЛЯ ПРИМЕНЕНИЯ В КАЧЕСТВЕ БИОЛЮМИНЕСЦЕНТНОГО РЕПОРТЕРА В ЖИВЫХ КЛЕТКАХ |
| JP6588917B2 (ja) | 2014-01-29 | 2019-10-09 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 生細胞への適用のためのプロ基質 |
| JP6703484B2 (ja) | 2014-01-29 | 2020-06-03 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 細胞による取り込み測定のための、標識用試薬としての、キノンでマスクされたプローブ |
| WO2016040788A1 (en) | 2014-09-11 | 2016-03-17 | Promega Corporation | Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence |
| EP3204509B1 (de) | 2014-10-08 | 2019-07-10 | Promega Corporation | Biolumineszierender test zum nachweis von succinat |
| RU2674894C2 (ru) * | 2017-01-30 | 2018-12-13 | Общество с ограниченной ответственностью "ПЛАНТА" | Новые люциферазы и способы их использования |
| GB201805396D0 (en) * | 2018-04-01 | 2018-05-16 | Univ College Cardiff Consultants Ltd | Modified Luciferases |
| CN110456052A (zh) * | 2019-08-22 | 2019-11-15 | 中国科学院武汉病毒研究所 | 一种血清中梅毒螺旋体抗体的快速检测方法及其应用 |
| US20230085844A1 (en) | 2020-02-14 | 2023-03-23 | Kikkoman Corporation | Liquid composition for measuring atp, and amp and/or adp in a sample |
| CN114015664B (zh) * | 2021-12-06 | 2023-07-21 | 郑州伊美诺生物技术有限公司 | 荧光素酶突变体及其应用 |
| CN114350627B (zh) * | 2021-12-16 | 2022-08-05 | 大连博格林生物科技有限公司 | 一种萤火虫荧光素酶突变体及其制备方法 |
| WO2024019165A1 (ja) | 2022-07-22 | 2024-01-25 | キッコーマン株式会社 | 微生物若しくは細胞又は微生物関連物質若しくは細胞関連物質を検出する方法及びキット |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3048466B2 (ja) * | 1991-06-27 | 2000-06-05 | キッコーマン株式会社 | 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法 |
| CA2157476C (en) * | 1994-01-03 | 2009-08-25 | Keith V. Wood | Mutant luciferases |
| GB9501170D0 (en) * | 1994-03-23 | 1995-03-08 | Secr Defence | Luciferases |
| AU707243B2 (en) * | 1995-01-20 | 1999-07-08 | Promega Corporation | Mutant luciferases |
| GB9707486D0 (en) * | 1997-04-11 | 1997-05-28 | Secr Defence | Enzyme assays |
| US6602677B1 (en) * | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
| AU754312C (en) * | 1997-09-19 | 2005-11-10 | Promega Corporation | Thermostable luciferases and methods of production |
| GB9823468D0 (en) * | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
-
2000
- 2000-10-26 CN CNB008177368A patent/CN100516223C/zh not_active Expired - Fee Related
- 2000-10-26 DE DE60036633T patent/DE60036633T2/de not_active Expired - Lifetime
- 2000-10-26 ES ES00971589T patent/ES2292479T3/es not_active Expired - Lifetime
- 2000-10-26 EP EP00971589A patent/EP1224294B1/de not_active Expired - Lifetime
- 2000-10-26 AT AT00971589T patent/ATE374820T1/de not_active IP Right Cessation
- 2000-10-26 CA CA002387691A patent/CA2387691A1/en not_active Abandoned
- 2000-10-26 RU RU2002113653/13A patent/RU2251571C2/ru not_active IP Right Cessation
- 2000-10-26 JP JP2001533163A patent/JP5154724B2/ja not_active Expired - Lifetime
- 2000-10-26 WO PCT/GB2000/004133 patent/WO2001031028A2/en active IP Right Grant
- 2000-10-26 NZ NZ518413A patent/NZ518413A/en unknown
- 2000-10-26 AU AU10425/01A patent/AU772485C/en not_active Ceased
- 2000-10-26 DK DK00971589T patent/DK1224294T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| JP5154724B2 (ja) | 2013-02-27 |
| DE60036633T2 (de) | 2008-07-03 |
| NZ518413A (en) | 2004-11-26 |
| EP1224294A2 (de) | 2002-07-24 |
| ES2292479T3 (es) | 2008-03-16 |
| WO2001031028A2 (en) | 2001-05-03 |
| RU2251571C2 (ru) | 2005-05-10 |
| CN1413252A (zh) | 2003-04-23 |
| DE60036633D1 (de) | 2007-11-15 |
| DK1224294T3 (da) | 2008-02-04 |
| CA2387691A1 (en) | 2001-05-03 |
| JP2003512071A (ja) | 2003-04-02 |
| AU772485B2 (en) | 2004-04-29 |
| EP1224294B1 (de) | 2007-10-03 |
| CN100516223C (zh) | 2009-07-22 |
| RU2002113653A (ru) | 2004-01-27 |
| WO2001031028A3 (en) | 2002-05-02 |
| AU772485C (en) | 2004-11-18 |
| AU1042501A (en) | 2001-05-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |