ATE374820T1 - Mutantenluciferase - Google Patents
MutantenluciferaseInfo
- Publication number
- ATE374820T1 ATE374820T1 AT00971589T AT00971589T ATE374820T1 AT E374820 T1 ATE374820 T1 AT E374820T1 AT 00971589 T AT00971589 T AT 00971589T AT 00971589 T AT00971589 T AT 00971589T AT E374820 T1 ATE374820 T1 AT E374820T1
- Authority
- AT
- Austria
- Prior art keywords
- luciferase
- amino acid
- compared
- wild
- type
- Prior art date
Links
- 108060001084 Luciferase Proteins 0.000 title abstract 9
- 239000005089 Luciferase Substances 0.000 title abstract 7
- 150000001413 amino acids Chemical class 0.000 abstract 3
- 230000002378 acidificating effect Effects 0.000 abstract 2
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 abstract 1
- 241000254064 Photinus pyralis Species 0.000 abstract 1
- 101000622060 Photinus pyralis Luciferin 4-monooxygenase Proteins 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 125000000539 amino acid group Chemical group 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000005516 coenzyme A Substances 0.000 abstract 1
- 229940093530 coenzyme a Drugs 0.000 abstract 1
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9925161.3A GB9925161D0 (en) | 1999-10-26 | 1999-10-26 | Novel enzyme |
| GBGB0016744.5A GB0016744D0 (en) | 1999-10-26 | 2000-07-10 | Novel enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE374820T1 true ATE374820T1 (de) | 2007-10-15 |
Family
ID=26244619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00971589T ATE374820T1 (de) | 1999-10-26 | 2000-10-26 | Mutantenluciferase |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP1224294B1 (de) |
| JP (1) | JP5154724B2 (de) |
| CN (1) | CN100516223C (de) |
| AT (1) | ATE374820T1 (de) |
| AU (1) | AU772485C (de) |
| CA (1) | CA2387691A1 (de) |
| DE (1) | DE60036633T2 (de) |
| DK (1) | DK1224294T3 (de) |
| ES (1) | ES2292479T3 (de) |
| NZ (1) | NZ518413A (de) |
| RU (1) | RU2251571C2 (de) |
| WO (1) | WO2001031028A2 (de) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6602677B1 (en) | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
| GB9823468D0 (en) * | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
| GB9925161D0 (en) * | 1999-10-26 | 1999-12-22 | Secr Defence | Novel enzyme |
| GB0111275D0 (en) | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| GB2400659B8 (en) | 2003-04-17 | 2007-07-09 | Cambrex Bio Science Nottingham | Assay methods and materials |
| WO2005035719A2 (en) * | 2003-10-03 | 2005-04-21 | Board Of Control Of Michigan Technological University | Modified luciferase |
| GB0517005D0 (en) | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| JP4999341B2 (ja) * | 2005-09-06 | 2012-08-15 | 株式会社バイオエネックス | 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法 |
| JP5224457B2 (ja) * | 2006-06-12 | 2013-07-03 | 独立行政法人産業技術総合研究所 | 変異型ルシフェラーゼ |
| JP5188771B2 (ja) | 2007-09-26 | 2013-04-24 | 株式会社日立製作所 | 変異型ホタルルシフェラーゼ |
| JP5753083B2 (ja) | 2008-07-22 | 2015-07-22 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | Ａｄｐ検出に基づく発光ホスホトランスフェラーゼまたはａｔｐヒドロラーゼのアッセイ法 |
| JP2011120559A (ja) | 2008-12-26 | 2011-06-23 | Kikkoman Corp | ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法 |
| WO2010127368A1 (en) * | 2009-05-01 | 2010-11-04 | Promega Corporation | Synthetic oplophorus luciferases with enhanced light output |
| WO2011002924A2 (en) * | 2009-06-30 | 2011-01-06 | The General Hospital Corporation | Gaussia luciferase variant for high-throughput screening |
| SG10202103336SA (en) | 2010-11-02 | 2021-04-29 | Promega Corp | Novel coelenterazine substrates and methods of use |
| AU2011323455B2 (en) | 2010-11-02 | 2017-07-27 | Promega Corporation | Coelenterazine derivatives and methods of using same |
| JP5896679B2 (ja) | 2011-03-15 | 2016-03-30 | オリンパス株式会社 | オオオバボタル由来ルシフェラーゼ |
| RU2495929C1 (ru) * | 2012-05-30 | 2013-10-20 | ФЕДЕРАЛЬНОЕ ГОСУДАРСТВЕННОЕ БЮДЖЕТНОЕ УЧРЕЖДЕНИЕ НАУКИ Институт биофизики Сибирского отделения Российской академии наук | УКОРОЧЕННАЯ МУТАНТНАЯ ЛЮЦИФЕРАЗА ИЗ Metridia longa ДЛЯ ПРИМЕНЕНИЯ В КАЧЕСТВЕ БИОЛЮМИНЕСЦЕНТНОГО РЕПОРТЕРА В ЖИВЫХ КЛЕТКАХ |
| JP6588917B2 (ja) | 2014-01-29 | 2019-10-09 | プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ | 生細胞への適用のためのプロ基質 |
| EP3099683B1 (de) | 2014-01-29 | 2020-08-05 | Promega Corporation | Chinonmaskierte sonden als markierungsreagenzien für zellaufnahmemessungen |
| US9732373B2 (en) | 2014-09-11 | 2017-08-15 | Promega Corporation | Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence |
| WO2016057084A1 (en) | 2014-10-08 | 2016-04-14 | Promega Corporation | Bioluminescent succinate detection assay |
| RU2674894C2 (ru) * | 2017-01-30 | 2018-12-13 | Общество с ограниченной ответственностью "ПЛАНТА" | Новые люциферазы и способы их использования |
| GB201805396D0 (en) * | 2018-04-01 | 2018-05-16 | Univ College Cardiff Consultants Ltd | Modified Luciferases |
| CN110456052A (zh) * | 2019-08-22 | 2019-11-15 | 中国科学院武汉病毒研究所 | 一种血清中梅毒螺旋体抗体的快速检测方法及其应用 |
| EP4105336A4 (de) | 2020-02-14 | 2024-04-10 | Kikkoman Corporation | Flüssige zusammensetzung zur messung von atp sowie amp und/oder adp in proben |
| CN114015664B (zh) * | 2021-12-06 | 2023-07-21 | 郑州伊美诺生物技术有限公司 | 荧光素酶突变体及其应用 |
| CN114350627B (zh) * | 2021-12-16 | 2022-08-05 | 大连博格林生物科技有限公司 | 一种萤火虫荧光素酶突变体及其制备方法 |
| EP4560025A1 (de) | 2022-07-22 | 2025-05-28 | Kikkoman Corporation | Verfahren und kit zum nachweis von mikroorganismen oder zellen oder nachweis von mikroorganismen oder zellverwandten substanzen |
| WO2024158050A1 (ja) | 2023-01-27 | 2024-08-02 | キッコーマン株式会社 | ルシフェラーゼ変異体 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3048466B2 (ja) * | 1991-06-27 | 2000-06-05 | キッコーマン株式会社 | 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法 |
| ATE391769T1 (de) * | 1994-01-03 | 2008-04-15 | Promega Corp | Mutierte luziferasen |
| GB9501170D0 (en) * | 1994-03-23 | 1995-03-08 | Secr Defence | Luciferases |
| AU707243B2 (en) * | 1995-01-20 | 1999-07-08 | Promega Corporation | Mutant luciferases |
| GB9707486D0 (en) * | 1997-04-11 | 1997-05-28 | Secr Defence | Enzyme assays |
| US6602677B1 (en) * | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
| AU754312C (en) * | 1997-09-19 | 2005-11-10 | Promega Corporation | Thermostable luciferases and methods of production |
| GB9823468D0 (en) * | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
-
2000
- 2000-10-26 CN CNB008177368A patent/CN100516223C/zh not_active Expired - Fee Related
- 2000-10-26 AU AU10425/01A patent/AU772485C/en not_active Ceased
- 2000-10-26 JP JP2001533163A patent/JP5154724B2/ja not_active Expired - Lifetime
- 2000-10-26 WO PCT/GB2000/004133 patent/WO2001031028A2/en not_active Ceased
- 2000-10-26 EP EP00971589A patent/EP1224294B1/de not_active Expired - Lifetime
- 2000-10-26 CA CA002387691A patent/CA2387691A1/en not_active Abandoned
- 2000-10-26 AT AT00971589T patent/ATE374820T1/de not_active IP Right Cessation
- 2000-10-26 DE DE60036633T patent/DE60036633T2/de not_active Expired - Lifetime
- 2000-10-26 ES ES00971589T patent/ES2292479T3/es not_active Expired - Lifetime
- 2000-10-26 DK DK00971589T patent/DK1224294T3/da active
- 2000-10-26 NZ NZ518413A patent/NZ518413A/en unknown
- 2000-10-26 RU RU2002113653/13A patent/RU2251571C2/ru not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2003512071A (ja) | 2003-04-02 |
| AU772485B2 (en) | 2004-04-29 |
| WO2001031028A2 (en) | 2001-05-03 |
| NZ518413A (en) | 2004-11-26 |
| RU2002113653A (ru) | 2004-01-27 |
| EP1224294A2 (de) | 2002-07-24 |
| CN1413252A (zh) | 2003-04-23 |
| ES2292479T3 (es) | 2008-03-16 |
| CN100516223C (zh) | 2009-07-22 |
| DE60036633D1 (de) | 2007-11-15 |
| WO2001031028A3 (en) | 2002-05-02 |
| DE60036633T2 (de) | 2008-07-03 |
| AU772485C (en) | 2004-11-18 |
| AU1042501A (en) | 2001-05-08 |
| EP1224294B1 (de) | 2007-10-03 |
| CA2387691A1 (en) | 2001-05-03 |
| JP5154724B2 (ja) | 2013-02-27 |
| RU2251571C2 (ru) | 2005-05-10 |
| DK1224294T3 (da) | 2008-02-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |