ATE391769T1 - Mutierte luziferasen - Google Patents

Mutierte luziferasen

Info

Publication number
ATE391769T1
ATE391769T1 AT95910074T AT95910074T ATE391769T1 AT E391769 T1 ATE391769 T1 AT E391769T1 AT 95910074 T AT95910074 T AT 95910074T AT 95910074 T AT95910074 T AT 95910074T AT E391769 T1 ATE391769 T1 AT E391769T1
Authority
AT
Austria
Prior art keywords
luciferases
dnas
wavelength
peak intensity
differs
Prior art date
Application number
AT95910074T
Other languages
English (en)
Inventor
Keith V Wood
Monika G Gruber
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ATE391769T1 publication Critical patent/ATE391769T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Luminescent Compositions (AREA)
AT95910074T 1994-01-03 1995-01-03 Mutierte luziferasen ATE391769T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US17708194A 1994-01-03 1994-01-03

Publications (1)

Publication Number Publication Date
ATE391769T1 true ATE391769T1 (de) 2008-04-15

Family

ID=22647117

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95910074T ATE391769T1 (de) 1994-01-03 1995-01-03 Mutierte luziferasen

Country Status (9)

Country Link
US (2) US6552179B1 (de)
EP (3) EP0689587B1 (de)
JP (2) JPH08510387A (de)
AT (1) ATE391769T1 (de)
AU (1) AU698424C (de)
CA (1) CA2157476C (de)
DE (1) DE69535744T2 (de)
ES (1) ES2301160T3 (de)
WO (1) WO1995018853A1 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2311525B (en) * 1995-01-20 1998-11-11 Secr Defence Mutant Luciferases
AU707243B2 (en) * 1995-01-20 1999-07-08 Promega Corporation Mutant luciferases
GB9707486D0 (en) * 1997-04-11 1997-05-28 Secr Defence Enzyme assays
US6602677B1 (en) * 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
EP1064360B1 (de) * 1998-03-27 2008-03-05 Prolume, Ltd. Luciferase, gfp fluoreszenzproteine, kodierende nukleinsaüre und ihre verwendung in der diagnose
EP1925320A3 (de) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferasen, fluoreszierende Proteine, Nukleinsäuren zur Kodierung der Luciferasen und der fluoreszierenden Proteine sowie deren Verwendung zur Diagnose, zum Screening mit hohem Durchsatz und zur Behandlung neuartiger Probleme
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
JP3694181B2 (ja) * 1999-01-05 2005-09-14 キッコーマン株式会社 変異型ルシフェラーゼ、変異型ルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び変異型ルシフェラーゼの製造法
CN100516223C (zh) * 1999-10-26 2009-07-22 3M创新有限公司 新型酶
GB9925161D0 (en) * 1999-10-26 1999-12-22 Secr Defence Novel enzyme
US7109315B2 (en) 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
US7879540B1 (en) * 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
EP1545705A4 (de) 2000-11-16 2010-04-28 Microspherix Llc Flexibler und/oder elastischer brachytherapie-seed oder strang
GB0030727D0 (en) 2000-12-15 2001-01-31 Lumitech Uk Ltd Methods and kits for detecting kinase activity
GB2400659B8 (en) 2003-04-17 2007-07-09 Cambrex Bio Science Nottingham Assay methods and materials
WO2005038029A2 (en) 2003-10-10 2005-04-28 Promega Corporation Luciferase biosensor
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US8227572B2 (en) * 2005-08-19 2012-07-24 Commonwealth Scientific And Industrial Research Organisation Arachnocampa luciferases
GB0517977D0 (en) * 2005-09-02 2005-10-12 Birkeland Innovasjon As Transgenic animal model for identifying modulators of protein kinase
EP2004813B1 (de) 2006-04-03 2015-06-03 Promega Corporation Permutierte und nichtpermutierte Luciferase-biosensoren, die zyklische Nukleotiden binden
WO2008109463A2 (en) * 2007-03-02 2008-09-12 University Of Massachusetts Red-shifted luciferase
WO2008109459A1 (en) * 2007-03-02 2008-09-12 University Of Massachusetts Luciferins
JP2008222084A (ja) * 2007-03-14 2008-09-25 Yamaha Motor Electronics Co Ltd 電動ゴルフカーのブレーキ劣化検出方法及びこれを用いた電動ゴルフカー
JP2008289475A (ja) * 2007-04-27 2008-12-04 National Institute Of Advanced Industrial & Technology 最大発光波長がシフトした変異型ルシフェラーゼ
JP6110591B2 (ja) 2008-05-19 2017-04-05 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ ｃＡＭＰのためのルシフェラーゼバイオセンサー
JP2010081908A (ja) * 2008-10-02 2010-04-15 Hitachi Ltd 変異型のホタルルシフェラーゼ
WO2010127368A1 (en) * 2009-05-01 2010-11-04 Promega Corporation Synthetic oplophorus luciferases with enhanced light output
US9290794B2 (en) 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
EP2990479B1 (de) 2010-05-11 2019-03-27 Promega Corporation Mutante protease-biosensoren mit verbesserten erkennungseigenschaften
AU2011323455B2 (en) 2010-11-02 2017-07-27 Promega Corporation Coelenterazine derivatives and methods of using same
SG10202103336SA (en) 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
JP6588917B2 (ja) 2014-01-29 2019-10-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生細胞への適用のためのプロ基質
EP3099683B1 (de) 2014-01-29 2020-08-05 Promega Corporation Chinonmaskierte sonden als markierungsreagenzien für zellaufnahmemessungen
US9732373B2 (en) 2014-09-11 2017-08-15 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
WO2016051517A1 (ja) 2014-09-30 2016-04-07 オリンパス株式会社 オレンジ色の発光を示すルシフェラーゼ
WO2018071807A2 (en) 2016-10-13 2018-04-19 Promega Corporation Thermal stable luciferase with improved resistance to inhibition by luciferin break-down products
JP6349003B1 (ja) 2017-03-17 2018-06-27 東洋ビーネット株式会社 耐熱性ルシフェラーゼ
WO2019236731A1 (en) 2018-06-05 2019-12-12 Promega Corporation Luciferase enzymes for use with thermostable luciferins in bioluminescent assays

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4968613A (en) 1987-07-29 1990-11-06 Kikkoman Corporation Luciferase gene and novel recombinant DNA as well as a method of producing luciferase
US5219737A (en) 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
US5229285A (en) 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly

Also Published As

Publication number Publication date
EP0689587A4 (de) 2001-08-08
EP1978092A1 (de) 2008-10-08
WO1995018853A1 (en) 1995-07-13
JP2009039129A (ja) 2009-02-26
CA2157476C (en) 2009-08-25
AU698424B2 (en) 1998-10-29
DE69535744T2 (de) 2009-07-02
AU1830395A (en) 1995-08-01
DE69535744D1 (de) 2008-05-21
CA2157476A1 (en) 1995-07-13
EP0689587B1 (de) 2008-04-09
ES2301160T3 (es) 2008-06-16
JP5090304B2 (ja) 2012-12-05
US6552179B1 (en) 2003-04-22
AU698424C (en) 2002-10-10
US20030166905A1 (en) 2003-09-04
EP1978091A1 (de) 2008-10-08
JPH08510387A (ja) 1996-11-05
EP0689587A1 (de) 1996-01-03

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