ATE144283T1 - Ergiebige herstellung von protease-mutanten - Google Patents

Ergiebige herstellung von protease-mutanten

Info

Publication number
ATE144283T1
ATE144283T1 AT90202109T AT90202109T ATE144283T1 AT E144283 T1 ATE144283 T1 AT E144283T1 AT 90202109 T AT90202109 T AT 90202109T AT 90202109 T AT90202109 T AT 90202109T AT E144283 T1 ATE144283 T1 AT E144283T1
Authority
AT
Austria
Prior art keywords
efficient production
protease mutants
proteases
bacillus
mutants
Prior art date
Application number
AT90202109T
Other languages
English (en)
Inventor
Der Laan Johannes Cornelis Van
Eekelen Christiaan Albertu Van
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8202457&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE144283(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Application granted granted Critical
Publication of ATE144283T1 publication Critical patent/ATE144283T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Mushroom Cultivation (AREA)
AT90202109T 1989-08-11 1990-08-02 Ergiebige herstellung von protease-mutanten ATE144283T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP89202117 1989-08-11

Publications (1)

Publication Number Publication Date
ATE144283T1 true ATE144283T1 (de) 1996-11-15

Family

ID=8202457

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90202109T ATE144283T1 (de) 1989-08-11 1990-08-02 Ergiebige herstellung von protease-mutanten

Country Status (19)

Country Link
US (1) US20040014175A1 (de)
EP (1) EP0414297B1 (de)
JP (1) JP3490435B2 (de)
KR (1) KR0169978B1 (de)
CN (1) CN1049866A (de)
AT (1) ATE144283T1 (de)
AU (1) AU629970B2 (de)
BG (1) BG60567B1 (de)
BR (1) BR9003956A (de)
CA (1) CA2023094C (de)
DD (1) DD297187A5 (de)
DE (1) DE69028890T2 (de)
DK (1) DK0414297T3 (de)
ES (1) ES2095233T3 (de)
FI (1) FI110365B (de)
GR (1) GR3021669T3 (de)
IE (1) IE77037B1 (de)
PT (1) PT94961B (de)
RU (1) RU2060276C1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4304161A1 (de) * 1993-02-12 1994-08-25 Solvay Enzymes Gmbh & Co Kg Verbesserte hochalkalische Proteasen
AU685638B2 (en) 1994-06-17 1998-01-22 Genencor International, Inc. Novel amylolytic enzymes derived from the b. licheniformis alpha-amylase, having improved characteristics
WO1997035956A1 (en) * 1996-03-27 1997-10-02 Novo Nordisk A/S Alkaline protease deficient filamentous fungi
JP3440694B2 (ja) * 1996-05-16 2003-08-25 ライオン株式会社 口腔用組成物
GB0024554D0 (en) 2000-10-06 2000-11-22 Agrol Ltd Ethanol production
AU2003228393A1 (en) 2002-03-29 2003-10-13 Genencor International, Inc. Ehanced protein expression in bacillus
ES2547866T3 (es) 2005-02-16 2015-10-09 The General Hospital Corporation Uso de proteínas de fusión de hemojuvelina para regular el metabolismo del hierro mediado por hepcidina
RU2510821C1 (ru) * 2012-10-24 2014-04-10 Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Казанский (Приволжский) Федеральный Университет" (ФГАОУ ВПО КФУ) Штамм бактерий bacillus pumilus мк-10 с низкой протеолитической активностью и его применение
RU2511416C1 (ru) * 2012-10-24 2014-04-10 Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Казанский (Приволжский) федеральный университет" (ФГАО ВПО КФУ) Штамм бактерий bacillus pumilus 2a-5 с низкой протеолитической активностью, повышенной активностью фосфатазы, способ его получения и применения

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1234445A (de) * 1967-10-03 1971-06-03
JPS6055118B2 (ja) * 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
ES2135386T3 (es) * 1987-02-27 1999-11-01 Genencor Int Transformacion de cepas de bacillus alcalofilas.
AU620326B2 (en) * 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
PT89702B (pt) * 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem

Also Published As

Publication number Publication date
DE69028890D1 (de) 1996-11-21
US20040014175A1 (en) 2004-01-22
IE77037B1 (en) 1997-11-19
BR9003956A (pt) 1991-09-03
FI903927A0 (fi) 1990-08-08
CA2023094A1 (en) 1991-02-12
KR0169978B1 (ko) 1999-02-01
EP0414297B1 (de) 1996-10-16
EP0414297A1 (de) 1991-02-27
AU6096190A (en) 1991-02-14
GR3021669T3 (en) 1997-02-28
ES2095233T3 (es) 1997-02-16
JP3490435B2 (ja) 2004-01-26
KR910004805A (ko) 1991-03-29
FI110365B (fi) 2002-12-31
BG60567B1 (bg) 1995-08-28
JPH03210177A (ja) 1991-09-13
RU2060276C1 (ru) 1996-05-20
DD297187A5 (de) 1992-01-02
DE69028890T2 (de) 1997-02-27
AU629970B2 (en) 1992-10-15
CN1049866A (zh) 1991-03-13
IE902904A1 (en) 1991-02-27
DK0414297T3 (da) 1997-07-07
CA2023094C (en) 2000-03-28
PT94961A (pt) 1991-04-18
PT94961B (pt) 1997-05-28

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EEIH Change in the person of patent owner
EELA Cancelled due to lapse of time