ATE124086T1 - Herstellung von proteinen. - Google Patents

Herstellung von proteinen.

Info

Publication number
ATE124086T1
ATE124086T1 AT89810335T AT89810335T ATE124086T1 AT E124086 T1 ATE124086 T1 AT E124086T1 AT 89810335 T AT89810335 T AT 89810335T AT 89810335 T AT89810335 T AT 89810335T AT E124086 T1 ATE124086 T1 AT E124086T1
Authority
AT
Austria
Prior art keywords
production
proteins
yeast strains
transformed
methods
Prior art date
Application number
AT89810335T
Other languages
English (en)
Inventor
Hansjoerg Dr Treichler
Kenji Dr Takabayashi
Dieter Heinrich Prof Dr Wolf
Jutta Dr Heim
Original Assignee
Ciba Geigy Ag
Ucp Gen Pharma Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888810524A external-priority patent/GB8810524D0/en
Priority claimed from GB888812627A external-priority patent/GB8812627D0/en
Priority claimed from GB898907110A external-priority patent/GB8907110D0/en
Application filed by Ciba Geigy Ag, Ucp Gen Pharma Ag filed Critical Ciba Geigy Ag
Application granted granted Critical
Publication of ATE124086T1 publication Critical patent/ATE124086T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Liquid Crystal Substances (AREA)
AT89810335T 1988-05-04 1989-05-01 Herstellung von proteinen. ATE124086T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB888810524A GB8810524D0 (en) 1988-05-04 1988-05-04 Improvements in production of polypeptides
GB888812627A GB8812627D0 (en) 1988-05-27 1988-05-27 Improvements in production of polypeptides
GB898907110A GB8907110D0 (en) 1988-05-04 1989-03-29 Improvements in the production of polypeptides

Publications (1)

Publication Number Publication Date
ATE124086T1 true ATE124086T1 (de) 1995-07-15

Family

ID=27263890

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89810335T ATE124086T1 (de) 1988-05-04 1989-05-01 Herstellung von proteinen.

Country Status (16)

Country Link
EP (1) EP0341215B1 (de)
JP (1) JPH0824565B2 (de)
AT (1) ATE124086T1 (de)
AU (1) AU614121B2 (de)
CA (1) CA1340016C (de)
DE (1) DE68923123T2 (de)
DK (1) DK175701B1 (de)
ES (1) ES2072918T3 (de)
FI (1) FI103129B1 (de)
HU (1) HU218731B (de)
IE (1) IE66044B1 (de)
IL (1) IL90170A (de)
MX (1) MX169508B (de)
NO (1) NO180593C (de)
NZ (1) NZ228955A (de)
PT (1) PT90436B (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
FR2645174B1 (fr) * 1989-03-31 1994-01-07 Transgene Sa Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
FR2645175B1 (fr) * 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche
US5879926A (en) * 1989-03-31 1999-03-09 Transgene S.A. Yeast strains for the production of mature heterologous proteins, especially hirudin
JP3187044B2 (ja) * 1989-12-01 2001-07-11 ビーエーエスエフ アクチェンゲゼルシャフト ヒルジン突然変異蛋白質及びヒルジンポリアルキレングリコール複合体
US5580734A (en) * 1990-07-13 1996-12-03 Transkaryotic Therapies, Inc. Method of producing a physical map contigous DNA sequences
ATE130049T1 (de) * 1990-07-13 1995-11-15 Transkaryotic Therapies Inc Verfahren zum absuchen einer bibliothek.
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
CA2090969C (en) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production of insulin-like growth factor-1 in methylotrophic yeast cells
ATE176500T1 (de) 1990-11-08 1999-02-15 Japan Energy Corp Sekretionsvektor, diesen enthaltene transformierte mikroorganismen und herstellung von produkten durch obigen mikroorganismus
SG84484A1 (en) * 1991-02-25 2001-11-20 Ciba Geigy Ag Improved yeast vectors
EP0578746B1 (de) * 1991-04-01 2002-07-03 Merck & Co., Inc. Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
HU214698B (hu) * 1992-04-09 1998-06-29 Gyógyszerkutató Intézet Kft. Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
DE69329540T2 (de) * 1992-09-04 2001-06-07 Novartis Ag, Basel Verfahren zur Herstellung von Proteaseinhibitoren
TW282490B (de) * 1992-12-15 1996-08-01 Ciba Geigy Ag
EP0663009A1 (de) * 1993-08-04 1995-07-19 Novartis AG Hochmolekulares desulphatohirudin
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
AU707657B2 (en) 1995-02-09 1999-07-15 Behringwerke Aktiengesellschaft Process for the production of proteins
WO1996029391A1 (en) * 1995-03-20 1996-09-26 Novo Nordisk A/S Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
DE19529997C1 (de) * 1995-08-16 1997-04-10 Hoechst Ag Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
WO1997012045A1 (en) * 1995-09-27 1997-04-03 Chiron Corporation Method of making a protease deficient host
EP0985513B1 (de) 1996-01-31 2003-01-02 Sumitomo Bakelite Company Limited Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung
FR2768743B1 (fr) 1997-09-23 2001-09-14 Bio Merieux Procede de lyse de micro-organisme
GB2338236B (en) 1998-06-13 2003-04-09 Aea Technology Plc Microbiological cell processing
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
CN102639559B (zh) * 2009-11-25 2015-04-29 诺沃—诺迪斯克有限公司 用于制备多肽的方法
KR102620914B1 (ko) 2017-10-10 2024-01-05 아지노모토 가부시키가이샤 단백질의 제조 방법
WO2021251383A1 (ja) 2020-06-11 2021-12-16 味の素株式会社 タンパク質の製造法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
JPS62224284A (ja) * 1986-03-25 1987-10-02 Agency Of Ind Science & Technol 微生物
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
GB8817160D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Novel proteins
GB8817161D0 (en) * 1988-07-19 1988-08-24 Ciba Geigy Ag Modified proteins

Also Published As

Publication number Publication date
FI103129B (fi) 1999-04-30
EP0341215B1 (de) 1995-06-21
IL90170A0 (en) 1989-12-15
IE66044B1 (en) 1995-12-13
DK175701B1 (da) 2005-01-24
PT90436A (pt) 1989-11-30
NO891837L (no) 1989-11-06
ES2072918T3 (es) 1995-08-01
FI892114A0 (fi) 1989-05-03
AU3382889A (en) 1989-11-09
EP0341215A2 (de) 1989-11-08
NO180593B (no) 1997-02-03
FI892114L (fi) 1989-11-05
IE891451L (en) 1989-11-04
CA1340016C (en) 1998-08-25
NO891837D0 (no) 1989-05-03
AU614121B2 (en) 1991-08-22
MX169508B (es) 1993-07-08
JPH02104279A (ja) 1990-04-17
DK217589A (da) 1989-11-05
EP0341215A3 (de) 1992-01-15
NZ228955A (en) 1991-06-25
HU218731B (hu) 2000-11-28
HUT54210A (en) 1991-01-28
NO180593C (no) 1997-05-14
JPH0824565B2 (ja) 1996-03-13
DE68923123D1 (de) 1995-07-27
FI103129B1 (fi) 1999-04-30
PT90436B (pt) 1994-11-30
DK217589D0 (da) 1989-05-03
DE68923123T2 (de) 1995-11-30
IL90170A (en) 1995-12-31

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Legal Events

Date Code Title Description
EELA Cancelled due to lapse of time