DE68923123T2 - Herstellung von Proteinen. - Google Patents
Herstellung von Proteinen.Info
- Publication number
- DE68923123T2 DE68923123T2 DE68923123T DE68923123T DE68923123T2 DE 68923123 T2 DE68923123 T2 DE 68923123T2 DE 68923123 T DE68923123 T DE 68923123T DE 68923123 T DE68923123 T DE 68923123T DE 68923123 T2 DE68923123 T2 DE 68923123T2
- Authority
- DE
- Germany
- Prior art keywords
- production
- proteins
- yeast strains
- transformed
- methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB888810524A GB8810524D0 (en) | 1988-05-04 | 1988-05-04 | Improvements in production of polypeptides |
GB888812627A GB8812627D0 (en) | 1988-05-27 | 1988-05-27 | Improvements in production of polypeptides |
GB898907110A GB8907110D0 (en) | 1988-05-04 | 1989-03-29 | Improvements in the production of polypeptides |
Publications (2)
Publication Number | Publication Date |
---|---|
DE68923123D1 DE68923123D1 (de) | 1995-07-27 |
DE68923123T2 true DE68923123T2 (de) | 1995-11-30 |
Family
ID=27263890
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE68923123T Expired - Lifetime DE68923123T2 (de) | 1988-05-04 | 1989-05-01 | Herstellung von Proteinen. |
Country Status (16)
Country | Link |
---|---|
EP (1) | EP0341215B1 (de) |
JP (1) | JPH0824565B2 (de) |
AT (1) | ATE124086T1 (de) |
AU (1) | AU614121B2 (de) |
CA (1) | CA1340016C (de) |
DE (1) | DE68923123T2 (de) |
DK (1) | DK175701B1 (de) |
ES (1) | ES2072918T3 (de) |
FI (1) | FI103129B1 (de) |
HU (1) | HU218731B (de) |
IE (1) | IE66044B1 (de) |
IL (1) | IL90170A (de) |
MX (1) | MX169508B (de) |
NO (1) | NO180593C (de) |
NZ (1) | NZ228955A (de) |
PT (1) | PT90436B (de) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8809860D0 (en) * | 1988-04-26 | 1988-06-02 | Ciba Geigy Ag | Process for production of polypeptides |
FR2645175B1 (fr) * | 1989-03-31 | 1994-02-18 | Transgene Sa | Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche |
FR2645174B1 (fr) * | 1989-03-31 | 1994-01-07 | Transgene Sa | Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant |
US5879926A (en) * | 1989-03-31 | 1999-03-09 | Transgene S.A. | Yeast strains for the production of mature heterologous proteins, especially hirudin |
EP0502962B1 (de) * | 1989-12-01 | 1996-03-13 | BASF Aktiengesellschaft | Hirudin-Muteine und deren Polyalkylenglykolkonjugate |
DE69114500T2 (de) * | 1990-07-13 | 1996-06-13 | Transkaryotic Therapies Inc | Verfahren zum absuchen einer bibliothek. |
US5580734A (en) * | 1990-07-13 | 1996-12-03 | Transkaryotic Therapies, Inc. | Method of producing a physical map contigous DNA sequences |
US5612198A (en) * | 1990-09-04 | 1997-03-18 | The Salk Institute | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
CA2090969C (en) * | 1990-09-04 | 2001-08-21 | Russell Arthur Brierley | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
CA2072375C (en) | 1990-11-08 | 2000-01-11 | Satoru Misawa | Hirudin analog, method of manufacturing thereof and anti-coagulant composition, and secretion vector, transformed microorganisms containing saidvector and manufacturing method of products which is produced from said microorganism |
SG84484A1 (en) * | 1991-02-25 | 2001-11-20 | Ciba Geigy Ag | Improved yeast vectors |
ATE220105T1 (de) * | 1991-04-01 | 2002-07-15 | Merck & Co Inc | Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung |
HU214698B (hu) * | 1992-04-09 | 1998-06-29 | Gyógyszerkutató Intézet Kft. | Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására |
DE69329540T2 (de) * | 1992-09-04 | 2001-06-07 | Novartis Ag | Verfahren zur Herstellung von Proteaseinhibitoren |
TW282490B (de) * | 1992-12-15 | 1996-08-01 | Ciba Geigy Ag | |
AU685835B2 (en) * | 1993-08-04 | 1998-01-29 | Novartis Ag | High molecular weight desulphatohirudin |
DE4404168A1 (de) * | 1994-02-10 | 1995-08-17 | Hoechst Ag | Hirudinderivate und Verfahren zu deren Herstellung |
JP3732516B2 (ja) * | 1995-02-09 | 2006-01-05 | ノバルティス・アクチエンゲゼルシャフト | タンパク質の製造法 |
US5861280A (en) * | 1995-03-20 | 1999-01-19 | Novo Nordisk A/S | Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production |
DE19529997C1 (de) | 1995-08-16 | 1997-04-10 | Hoechst Ag | Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen |
AU7112796A (en) * | 1995-09-27 | 1997-04-17 | Chiron Corporation | Method of making a protease deficient host |
DE69624087T2 (de) | 1996-01-31 | 2003-06-05 | Sumitomo Bakelite Co | Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung |
FR2768743B1 (fr) * | 1997-09-23 | 2001-09-14 | Bio Merieux | Procede de lyse de micro-organisme |
GB2338236B (en) | 1998-06-13 | 2003-04-09 | Aea Technology Plc | Microbiological cell processing |
US7795205B2 (en) | 2004-04-12 | 2010-09-14 | Canyon Pharmaceuticals, Inc. | Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor |
CN102639559B (zh) | 2009-11-25 | 2015-04-29 | 诺沃—诺迪斯克有限公司 | 用于制备多肽的方法 |
KR102620914B1 (ko) | 2017-10-10 | 2024-01-05 | 아지노모토 가부시키가이샤 | 단백질의 제조 방법 |
CN115836127A (zh) | 2020-06-11 | 2023-03-21 | 味之素株式会社 | 蛋白的制造方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3342139A1 (de) * | 1983-11-22 | 1985-05-30 | Ciba-Geigy Ag, Basel | Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel |
JPS62224284A (ja) * | 1986-03-25 | 1987-10-02 | Agency Of Ind Science & Technol | 微生物 |
GB8809860D0 (en) * | 1988-04-26 | 1988-06-02 | Ciba Geigy Ag | Process for production of polypeptides |
GB8817160D0 (en) * | 1988-07-19 | 1988-08-24 | Ciba Geigy Ag | Novel proteins |
GB8817161D0 (en) * | 1988-07-19 | 1988-08-24 | Ciba Geigy Ag | Modified proteins |
-
1989
- 1989-04-28 AU AU33828/89A patent/AU614121B2/en not_active Expired
- 1989-05-01 DE DE68923123T patent/DE68923123T2/de not_active Expired - Lifetime
- 1989-05-01 AT AT89810335T patent/ATE124086T1/de not_active IP Right Cessation
- 1989-05-01 ES ES89810335T patent/ES2072918T3/es not_active Expired - Lifetime
- 1989-05-01 EP EP89810335A patent/EP0341215B1/de not_active Expired - Lifetime
- 1989-05-02 JP JP1112294A patent/JPH0824565B2/ja not_active Expired - Lifetime
- 1989-05-02 MX MX015887A patent/MX169508B/es unknown
- 1989-05-02 NZ NZ228955A patent/NZ228955A/en unknown
- 1989-05-02 CA CA000598488A patent/CA1340016C/en not_active Expired - Lifetime
- 1989-05-02 PT PT90436A patent/PT90436B/pt not_active IP Right Cessation
- 1989-05-03 IE IE145189A patent/IE66044B1/en not_active IP Right Cessation
- 1989-05-03 IL IL9017089A patent/IL90170A/en active IP Right Grant
- 1989-05-03 DK DK198902175A patent/DK175701B1/da not_active IP Right Cessation
- 1989-05-03 FI FI892114A patent/FI103129B1/fi not_active IP Right Cessation
- 1989-05-03 NO NO891837A patent/NO180593C/no not_active IP Right Cessation
- 1989-05-03 HU HU077/89A patent/HU218731B/hu unknown
Also Published As
Publication number | Publication date |
---|---|
AU3382889A (en) | 1989-11-09 |
EP0341215B1 (de) | 1995-06-21 |
DE68923123D1 (de) | 1995-07-27 |
PT90436B (pt) | 1994-11-30 |
NO180593B (no) | 1997-02-03 |
AU614121B2 (en) | 1991-08-22 |
EP0341215A3 (de) | 1992-01-15 |
JPH02104279A (ja) | 1990-04-17 |
ATE124086T1 (de) | 1995-07-15 |
IL90170A (en) | 1995-12-31 |
FI103129B (fi) | 1999-04-30 |
EP0341215A2 (de) | 1989-11-08 |
HU218731B (hu) | 2000-11-28 |
DK217589D0 (da) | 1989-05-03 |
FI892114A (fi) | 1989-11-05 |
DK175701B1 (da) | 2005-01-24 |
ES2072918T3 (es) | 1995-08-01 |
NO891837L (no) | 1989-11-06 |
IE66044B1 (en) | 1995-12-13 |
NO180593C (no) | 1997-05-14 |
PT90436A (pt) | 1989-11-30 |
DK217589A (da) | 1989-11-05 |
FI103129B1 (fi) | 1999-04-30 |
CA1340016C (en) | 1998-08-25 |
IE891451L (en) | 1989-11-04 |
NZ228955A (en) | 1991-06-25 |
HUT54210A (en) | 1991-01-28 |
NO891837D0 (no) | 1989-05-03 |
IL90170A0 (en) | 1989-12-15 |
MX169508B (es) | 1993-07-08 |
FI892114A0 (fi) | 1989-05-03 |
JPH0824565B2 (ja) | 1996-03-13 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: NOVARTIS AG, BASEL, CH UCP GEN-PHARMA AG, ZUERICH, |
|
8328 | Change in the person/name/address of the agent |
Representative=s name: PFENNING MEINIG & PARTNER GBR, 80339 MUENCHEN |