ATE220105T1 - Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung - Google Patents

Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung

Info

Publication number
ATE220105T1
ATE220105T1 AT92910129T AT92910129T ATE220105T1 AT E220105 T1 ATE220105 T1 AT E220105T1 AT 92910129 T AT92910129 T AT 92910129T AT 92910129 T AT92910129 T AT 92910129T AT E220105 T1 ATE220105 T1 AT E220105T1
Authority
AT
Austria
Prior art keywords
pichia
proteolytic activity
genes
genes affecting
strains
Prior art date
Application number
AT92910129T
Other languages
English (en)
Inventor
Martin Anthony Gleeson
Bradley Drake Howard
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24724845&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE220105(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Application granted granted Critical
Publication of ATE220105T1 publication Critical patent/ATE220105T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/938—Pichia

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT92910129T 1991-04-01 1992-03-31 Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung ATE220105T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67891691A 1991-04-01 1991-04-01
PCT/US1992/002521 WO1992017595A1 (en) 1991-04-01 1992-03-31 Genes which influence pichia proteolytic activity, and uses therefor

Publications (1)

Publication Number Publication Date
ATE220105T1 true ATE220105T1 (de) 2002-07-15

Family

ID=24724845

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92910129T ATE220105T1 (de) 1991-04-01 1992-03-31 Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung

Country Status (9)

Country Link
US (5) US5324660A (de)
EP (1) EP0578746B1 (de)
JP (2) JPH06506117A (de)
AT (1) ATE220105T1 (de)
AU (1) AU661844B2 (de)
CA (1) CA2105064A1 (de)
DE (1) DE69232666T2 (de)
ES (1) ES2177528T3 (de)
WO (1) WO1992017595A1 (de)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
JPH06506117A (ja) * 1991-04-01 1994-07-14 メルク　エンド　カンパニー　インコーポレーテッド ピキア（Ｐｉｃｈｉａ）蛋白質分解活性に影響する遺伝子およびその使用
FR2692907B1 (fr) 1992-06-25 1995-06-30 Rhone Poulenc Rorer Sa Levures kluyveromyces modifiees, preparation et utilisation.
US5674728A (en) * 1993-11-03 1997-10-07 Novartis Corporation Aspergillus niger vacuolar aspartyl protease
AU2733895A (en) * 1994-06-17 1996-01-15 Novo Nordisk A/S A fungus wherein the area gene has been modified and an area gene from aspergillus oryzae
US5594119A (en) * 1994-09-20 1997-01-14 Novo Nordisk A/S Gene encoding carboxypeptidase of aspergillus niger
JP3974171B2 (ja) * 1995-03-20 2007-09-12 ノボザイムス アクティーゼルスカブ 低下したレベルのメタロプロテアーゼを発見する宿主細胞及びその細胞をタンパク質の生産に用いる方法
US6107057A (en) * 1995-09-25 2000-08-22 Chiron Corporation Pichia secretory leader for protein expression
US6153424A (en) * 1995-11-09 2000-11-28 Zymogenetics, Inc. Protease-deficient strains of Pichia methanolica
CN1160465C (zh) * 1995-12-15 2004-08-04 诺沃奇梅兹有限公司 其中areA、pepC和/或pepE基因已被灭活的真菌
CN1120235C (zh) 1996-03-27 2003-09-03 诺沃奇梅兹有限公司 碱性蛋白酶缺陷型丝状真菌
US6110703A (en) * 1996-07-05 2000-08-29 Novo Nordisk A/S Method for the production of polypeptides
AU4200897A (en) * 1996-09-19 1998-04-14 Novo Nordisk A/S Novel host cells and methods of producing proteins
EP1003889A1 (de) 1997-08-05 2000-05-31 Chiron Corporation Gensequenzen aus pichia pastoris und deren verwendung
WO1999014347A1 (en) * 1997-09-15 1999-03-25 Zymogenetics, Inc. PROTEASE-DEFICIENT STRAINS OF $i(PICHIA METHANOLICA)
US6183953B1 (en) 1997-09-15 2001-02-06 Zymogenetics, Inc. Chromosomal mutagenesis in Pichia methanolica
ATE310815T1 (de) * 1997-09-15 2005-12-15 Zymogenetics Inc Chromosomale mutagenese in pichia methanolica
WO2001090385A2 (en) 2000-05-25 2001-11-29 Chiron Corporation IRON REGULATION OF THE PpSEC10 TRANSCRIPTIONAL REGULATORY REGION OF PICHIA PASTORIS AND METHODS OF USE
US20060257399A1 (en) * 2000-06-28 2006-11-16 Glycofi, Inc. Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform
US20060034828A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAcMAN5GLCNAC2 glycoform
US20060029604A1 (en) * 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
US20060034830A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GalGlcNAcMan5GLcNAc2 glycoform
WO2002101038A1 (fr) 2001-05-29 2002-12-19 Asahi Glass Company, Limited Procede de construction d'un hote et procede de production d'une proteine etrangere
US20060034829A1 (en) * 2001-12-27 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a MAN3GLCNAC2 glycoform
US20060024292A1 (en) * 2001-12-27 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Gal2GlcNAc2Man3GlcNAc2 glycoform
ATE420198T1 (de) * 2002-04-26 2009-01-15 Kirin Pharma Kk Methylotrophe hefe,die eine zuckerkette eines säugers herstellt
US7514253B2 (en) * 2003-05-16 2009-04-07 Glycofi, Inc. URA5 gene and methods for stable genetic integration in yeast
ES2393555T3 (es) 2003-10-22 2012-12-26 Keck Graduate Institute Métodos para la síntesis de polipéptidos hetero-multiméricos en levaduras usando una estrategia de apareamiento haploide.
CN101006174B (zh) 2004-06-21 2010-05-26 诺维信公司 以相同取向稳定保持的至少两个orf的多拷贝
CA2531616A1 (en) * 2004-12-28 2006-06-28 Kabushiki Kaisha Kobe Seiko Sho (Kobe Steel, Ltd.) High strength thin steel sheet having high hydrogen embrittlement resisting property and high workability
EP1937305A4 (de) * 2005-09-09 2008-10-08 Glycofi Inc Immunglobulin, das vorwiegend eine man7glcnac2, man8glcnac2 glycoform enthält
EP2401357B1 (de) * 2009-02-26 2015-03-25 GlaxoSmithKline LLC Wirtszellen und verfahren zu ihrer verwendung
US20120213728A1 (en) * 2009-10-30 2012-08-23 Merck Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris
US8703444B2 (en) 2009-12-14 2014-04-22 Centre Hospitalier Universitaire Vaudois (Chuv) Pichia pastoris deficient in endogenous secreted protease
BR112013008347A2 (pt) 2010-10-06 2016-06-14 Bp Corp North America Inc polipeptídeos de variantes cbh i
US12404309B2 (en) 2018-02-12 2025-09-02 Lonza Ltd Host cell for producing a protein of interest
WO2020106599A1 (en) * 2018-11-19 2020-05-28 Basf Se A recombinant yeast cell
KR102699074B1 (ko) 2019-01-11 2024-08-26 론자 리미티드 재조합 숙주 세포에서 탄소 공급원 조절된 단백질 생산
JP7570346B2 (ja) 2019-04-01 2024-10-21 ウニヴェルズィテート・フューア・ボーデンクルトゥーア・ウィーン Ｍｕｔ－メチロトローフ酵母
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
WO2021198431A1 (en) 2020-04-01 2021-10-07 Lonza Ltd Helper factors for expressing proteins in yeast
IL301312A (en) 2020-09-30 2023-05-01 Lonza Ag Host cells overexpressing translational factors
EP4638756A1 (de) 2022-12-20 2025-10-29 Lonza Ltd. Wirtszellen mit pat1-knockout für erhöhte spezifische proteinproduktivität

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
DE3486216T2 (de) * 1983-04-25 1994-02-17 Chiron Corp Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
IL71991A (en) * 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
US4870008A (en) * 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
GB8327880D0 (en) * 1983-10-18 1983-11-16 Ajinomoto Kk Saccharomyces cerevisiae
US4839295A (en) * 1984-06-08 1989-06-13 Pierce Chemical Company Measurement of protein using bicinchoninic acid
CA1341130C (en) * 1984-07-27 2000-10-31 Adrianus Marinus Ledeboer Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
GB8521496D0 (en) * 1985-08-29 1985-10-02 Ciba Geigy Ag Repressible yeast promoters
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
US4812405A (en) * 1986-02-18 1989-03-14 Phillips Petroleum Company Double auxotrophic mutants of Pichia pastoris and methods for preparation
GB2188933A (en) * 1986-04-10 1987-10-14 Bayer Ag Expression vectors for production of polypeptides, method for enhanced expression of polypeptides, hosts containing the expression vectors, products manufactured thereby
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
US4929555A (en) * 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
KR890701606A (ko) * 1987-11-02 1989-12-21 인더스트리얼 어소시에이츠 인코포레이팃드 피치아 파스토리스로부터의 분비를 통한 동물 리소자임의 생산 및 이를 위한 조성물
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
ATE248925T1 (de) * 1988-01-05 2003-09-15 Roche Diagnostics Gmbh Verfahren zur herstellung von proteinen oder proteinhaltigen genprodukten
DE68922014T2 (de) * 1988-01-27 1995-08-03 M & D Research Co Protease.
AU614121B2 (en) * 1988-05-04 1991-08-22 Novartis Ag Improvements in the production of polypeptides
WO1990000191A1 (en) * 1988-07-01 1990-01-11 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of rabies glycoprotein g and composition therefor
GB8819826D0 (en) * 1988-08-20 1988-09-21 Kabivitrum Ab Glycosylated igf-1
IL91512A0 (en) * 1988-09-09 1990-04-29 Salk Inst Biotech Ind Production of interluekin-2 poly-peptides in pichia pastoris yeast cells
AU4332589A (en) * 1988-09-26 1990-04-18 Salk Institute Biotechnology/Industrial Associates, Inc., The Mixed feed recombinant yeast fermentation
US5204252A (en) * 1989-02-08 1993-04-20 Henkel Research Corporation Candida tropicalis transformation system
WO1990009434A1 (en) * 1989-02-13 1990-08-23 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of superoxide dismutase in pichia pastoris yeast cells
FR2645175B1 (fr) * 1989-03-31 1994-02-18 Transgene Sa Souche de saccharomyces cerevisiaeproductrice d'une proteine heterologue et procede de preparation de ladite proteine heterologue par fermentation de ladite souche
US5122465A (en) * 1989-06-12 1992-06-16 Phillips Petroleum Company Strains of pichia pastoris created by interlocus recombination
US5231010A (en) * 1989-09-13 1993-07-27 Bayer Aktiengesellschaft Recombinant aprotinin variants genetically engineered process for the microbial preparation of homogeneously processed aprotinin variants and the therapeutic use thereof
WO1991005057A1 (en) * 1989-09-28 1991-04-18 The Salk Institute Biotechnology/Industrial Associates, Inc. Cd4 production in pichia pastoris
US5258302A (en) * 1990-07-03 1993-11-02 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA for expression of aprotinin in methylotrophic yeast cells
CA2090969C (en) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production of insulin-like growth factor-1 in methylotrophic yeast cells
EP0547171A1 (de) * 1990-09-05 1993-06-23 The Salk Institute Biotechnology Industrial Associates, Inc. Produktion menschlichen lysozyms in methylotrophen hefezellen und seine effiziente sekretion aus diesen
JPH07108232B2 (ja) * 1990-10-09 1995-11-22 エム・ディ・リサーチ株式会社 ペプチド又は蛋白質の製造方法
US5231178A (en) * 1991-01-16 1993-07-27 The Salk Institute Biotechnology/Industrial Associates, Inc. Method for the purification of intact, correctly-folded insulin-like growth factor-1
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
JPH06506117A (ja) * 1991-04-01 1994-07-14 メルク　エンド　カンパニー　インコーポレーテッド ピキア（Ｐｉｃｈｉａ）蛋白質分解活性に影響する遺伝子およびその使用
US5213010A (en) * 1991-05-07 1993-05-25 Toshiba Kikai Kabushiki Kaisha Driver power transmitting apparatus of twin shaft extruders
WO1993009233A2 (en) * 1991-10-31 1993-05-13 The Salk Institute Biotechnology/Industrial Associates Inc. Recombinant amyloid precursor protein inhibitor domain and treatment of various disease states

Also Published As

Publication number Publication date
JP2003159090A (ja) 2003-06-03
DE69232666T2 (de) 2003-03-20
ES2177528T3 (es) 2002-12-16
EP0578746A1 (de) 1994-01-19
US5691166A (en) 1997-11-25
US6051419A (en) 2000-04-18
JPH06506117A (ja) 1994-07-14
US5541112A (en) 1996-07-30
CA2105064A1 (en) 1992-10-02
AU1750592A (en) 1992-11-02
US5831053A (en) 1998-11-03
US5324660A (en) 1994-06-28
DE69232666D1 (de) 2002-08-08
AU661844B2 (en) 1995-08-10
EP0578746B1 (de) 2002-07-03
WO1992017595A1 (en) 1992-10-15

Similar Documents

Publication Publication Date Title
DE69232666D1 (de) Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
ATE294867T1 (de) Mutierte alpha-amylase aus bacillus licheniformis,die eine modifizierung von resten a210, h405 und/oder t412 enthält
FI89076B (fi) Alfa-amylasblandningar foer att oeverfoera staerkelse i vaetskeform och foerfarande foer oeverfoering av staerkelse i flytande form
ATE110419T1 (de) Nachweis von listeria.
FI971953A0 (fi) Proteiinityrosiinikinaasin välittämää soluproliferaatiota inhiboivat 6-aryyli-pyrido(2,3-d)pyrimidiinit ja naftyridiinit
DE69115280D1 (de) Indolderivate, die die leukotrienbiosynthese hemmen.
ES2123006T3 (es) Levaduras recombinantes altamente estables para la produccion de proteinas recombinantes.
MY110664A (en) Protein c derivatives
TR199903213T2 (xx) Pirimidin-2,4,6-trion t�revleri, bunlar�n �retimi i�in i�lemler ve bu bile�ikleri ihtiva eden farmas�tik preparasyonlar.
ATE214610T1 (de) Neuartige pharmazeutische verwendungen von krill- enzymen
KR970009949B1 (en) Novel serine proteolytic enzyme and its process
KR880701728A (ko) 조직-형 및 우로키나제-형 플라스 미노겐 활성체 억제제의 진단검정 방법 및 억제제의 암호화 유전자
DE69031271D1 (de) Varianten von pai-2
FI875324A0 (fi) Hybridproteiner.
AU669149B2 (en) Expression signal-peptide-free staphylokinases
IT1262954B (it) Regioni antigeniche dei complessi tpl e anticorpi diretti contro di essi.
MX9304070A (es) Piperidilamidas, piperidilsulfonamidas y piperidilsulfoxamidas novedosas como inhibidores de la biosintesis del colesterol.
ATE226591T1 (de) Oligopeptide hergeleitet von fragmenten des c- reaktiven proteins
DK0713365T4 (da) Rugmel
ES2042988T3 (es) Acidos 1h-indazol-3-aceticos como inhibidores de aldosareductasa.
DE69728165D1 (de) Cycloalkyl inhibitoren der protein-farnesyltransferase
ES2037603A1 (es) Procedimiento para la obtencion de preparados de enzimas, sin agentes superficie activos, pulverulentos o granulados conteniendo proteasa como enzima activa.
DE69333261D1 (de) KLONIERUNG UND EXPRESSION VON DEM beta APP-C100 REZEPTOR
DE59408793D1 (de) Galenische Zubereitungen von Molsidomin
ATE102214T1 (de) Homocyclostatin und cyclostatin enthaltende polypeptide als antihypertensiva.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties