ATE220105T1 - Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung - Google Patents

Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung

Info

Publication number
ATE220105T1
ATE220105T1 AT92910129T AT92910129T ATE220105T1 AT E220105 T1 ATE220105 T1 AT E220105T1 AT 92910129 T AT92910129 T AT 92910129T AT 92910129 T AT92910129 T AT 92910129T AT E220105 T1 ATE220105 T1 AT E220105T1
Authority
AT
Austria
Prior art keywords
pichia
proteolytic activity
genes
genes affecting
strains
Prior art date
Application number
AT92910129T
Other languages
English (en)
Inventor
Martin Anthony Gleeson
Bradley Drake Howard
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24724845&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE220105(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Application granted granted Critical
Publication of ATE220105T1 publication Critical patent/ATE220105T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/938—Pichia

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT92910129T 1991-04-01 1992-03-31 Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung ATE220105T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67891691A 1991-04-01 1991-04-01
PCT/US1992/002521 WO1992017595A1 (en) 1991-04-01 1992-03-31 Genes which influence pichia proteolytic activity, and uses therefor

Publications (1)

Publication Number Publication Date
ATE220105T1 true ATE220105T1 (de) 2002-07-15

Family

ID=24724845

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92910129T ATE220105T1 (de) 1991-04-01 1992-03-31 Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung

Country Status (9)

Country Link
US (5) US5324660A (de)
EP (1) EP0578746B1 (de)
JP (2) JPH06506117A (de)
AT (1) ATE220105T1 (de)
AU (1) AU661844B2 (de)
CA (1) CA2105064A1 (de)
DE (1) DE69232666T2 (de)
ES (1) ES2177528T3 (de)
WO (1) WO1992017595A1 (de)

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FR2692907B1 (fr) 1992-06-25 1995-06-30 Rhone Poulenc Rorer Sa Levures kluyveromyces modifiees, preparation et utilisation.
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AU7245096A (en) * 1995-09-25 1997-04-17 Chiron Corporation Pichia secretory leader for protein expression
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AU1092897A (en) * 1995-12-15 1997-07-14 Novo Nordisk A/S A funguns wherein the area, pepc and/or pepe genes have been inactivated
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US6110703A (en) * 1996-07-05 2000-08-29 Novo Nordisk A/S Method for the production of polypeptides
DK0956338T3 (da) 1996-09-19 2006-05-08 Novozymes As Værtsceller og fremgangsmåder til produktion af proteiner
AU8688798A (en) 1997-08-05 1999-03-01 Chiron Corporation Novel (pichia pastoris) gene sequences and methods for their use
WO1999014320A1 (en) * 1997-09-15 1999-03-25 Zymogenetics, Inc. CHROMOSOMAL MUTAGENESIS IN $i(PICHIA METHANOLICA)
AU9231898A (en) * 1997-09-15 1999-04-05 Zymogenetics Inc. Protease-deficient strains of (pichia methanolica)
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US6605450B1 (en) 2000-05-25 2003-08-12 Chiron Corporation Iron regulation of the PpSEC10 transcriptional regulatory region of Pichia pastoris and methods of use
US20060257399A1 (en) * 2000-06-28 2006-11-16 Glycofi, Inc. Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform
US20060034830A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GalGlcNAcMan5GLcNAc2 glycoform
US20060034828A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAcMAN5GLCNAC2 glycoform
US20060029604A1 (en) * 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
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US20060024292A1 (en) * 2001-12-27 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Gal2GlcNAc2Man3GlcNAc2 glycoform
US20060034829A1 (en) * 2001-12-27 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a MAN3GLCNAC2 glycoform
EP1505149B1 (de) 2002-04-26 2009-01-07 Kirin Pharma Kabushiki Kaisha Methylotrophe hefe,die eine zuckerkette eines säugers herstellt
US7514253B2 (en) * 2003-05-16 2009-04-07 Glycofi, Inc. URA5 gene and methods for stable genetic integration in yeast
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WO2019154686A1 (en) 2018-02-12 2019-08-15 Lonza Ltd Host cell for producing a protein of interest
EP3884037A1 (de) * 2018-11-19 2021-09-29 Basf Se Rekombinante hefezelle
EP3874047B2 (de) 2019-01-11 2024-12-25 Lonza Ltd. Kohlenstoffquellenregulierte proteinproduktion in einer rekombinanten wirtszelle
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
AU2020252241B2 (en) 2019-04-01 2024-03-21 Universität Für Bodenkultur Wien Mut- methylotrophic yeast
EP4127185B1 (de) 2020-04-01 2025-06-04 Lonza Ltd. Helferfaktoren zur expression von proteinen in hefe
CN116490517A (zh) 2020-09-30 2023-07-25 龙沙有限公司 过表达翻译因子的宿主细胞
WO2024133538A1 (en) 2022-12-20 2024-06-27 Lonza Ltd Host cells with pat1 knockout for increased specific protein productivity

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Also Published As

Publication number Publication date
US5541112A (en) 1996-07-30
US5324660A (en) 1994-06-28
EP0578746B1 (de) 2002-07-03
US6051419A (en) 2000-04-18
ES2177528T3 (es) 2002-12-16
JPH06506117A (ja) 1994-07-14
WO1992017595A1 (en) 1992-10-15
JP2003159090A (ja) 2003-06-03
US5831053A (en) 1998-11-03
CA2105064A1 (en) 1992-10-02
DE69232666D1 (de) 2002-08-08
AU1750592A (en) 1992-11-02
EP0578746A1 (de) 1994-01-19
DE69232666T2 (de) 2003-03-20
AU661844B2 (en) 1995-08-10
US5691166A (en) 1997-11-25

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