ES2177528T3 - Genes que influyen en la actividad proteolitica de pichia y usoso de los mismos. - Google Patents

Genes que influyen en la actividad proteolitica de pichia y usoso de los mismos.

Info

Publication number
ES2177528T3
ES2177528T3 ES92910129T ES92910129T ES2177528T3 ES 2177528 T3 ES2177528 T3 ES 2177528T3 ES 92910129 T ES92910129 T ES 92910129T ES 92910129 T ES92910129 T ES 92910129T ES 2177528 T3 ES2177528 T3 ES 2177528T3
Authority
ES
Spain
Prior art keywords
genes
pichia
themselves
influence
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92910129T
Other languages
English (en)
Inventor
Martin Anthony Gleeson
Bradley Drake Howard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Application granted granted Critical
Publication of ES2177528T3 publication Critical patent/ES2177528T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/938—Pichia

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

SE DESCRIBEN EL AISLAMIENTO Y LA CARACTERIZACION DE GENES IMPLICADOS EN EL PROCESAMIENTO PROTEOLITICO EN ESPECIES DEL GENES PICHIA. LA DISPONIBILIDAD DE TALES GENES HA FACULTADO LA GENERACION DE RAZAS DE PICHIA QUE SON DEFICIENTES EN LA ACTIVIDAD PROTEOLITICA Y UTILES COMO HUESPEDES PARA LA EXPRESION DE PRODUCTOS RECOMBINANTES PROTEOLITICAMENTE SENSIBLES. TAMBIEN SE DESCRIBEN EL AISLAMIENTO Y LA CARACTERIZACION DE GENES ADICIONALES PROCEDENTES DE ESPECIES DEL GENE PICHIA, ASI COMO USOS PARA ESTO.
ES92910129T 1991-04-01 1992-03-31 Genes que influyen en la actividad proteolitica de pichia y usoso de los mismos. Expired - Lifetime ES2177528T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US67891691A 1991-04-01 1991-04-01

Publications (1)

Publication Number Publication Date
ES2177528T3 true ES2177528T3 (es) 2002-12-16

Family

ID=24724845

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92910129T Expired - Lifetime ES2177528T3 (es) 1991-04-01 1992-03-31 Genes que influyen en la actividad proteolitica de pichia y usoso de los mismos.

Country Status (9)

Country Link
US (5) US5324660A (es)
EP (1) EP0578746B1 (es)
JP (2) JPH06506117A (es)
AT (1) ATE220105T1 (es)
AU (1) AU661844B2 (es)
CA (1) CA2105064A1 (es)
DE (1) DE69232666T2 (es)
ES (1) ES2177528T3 (es)
WO (1) WO1992017595A1 (es)

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FR2692907B1 (fr) 1992-06-25 1995-06-30 Rhone Poulenc Rorer Sa Levures kluyveromyces modifiees, preparation et utilisation.
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DK0770139T3 (da) * 1994-06-17 2004-04-13 Novozymes As En svamp, hvor areA-genet er blevet modificeret, samt et areA-gen fra aspergillus oryzae
US5594119A (en) * 1994-09-20 1997-01-14 Novo Nordisk A/S Gene encoding carboxypeptidase of aspergillus niger
EP0815200B1 (en) * 1995-03-20 2007-02-14 Novozymes A/S Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
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US6153424A (en) * 1995-11-09 2000-11-28 Zymogenetics, Inc. Protease-deficient strains of Pichia methanolica
CN1160465C (zh) * 1995-12-15 2004-08-04 诺沃奇梅兹有限公司 其中areA、pepC和/或pepE基因已被灭活的真菌
CN1120235C (zh) 1996-03-27 2003-09-03 诺沃奇梅兹有限公司 碱性蛋白酶缺陷型丝状真菌
US6110703A (en) * 1996-07-05 2000-08-29 Novo Nordisk A/S Method for the production of polypeptides
ATE313620T1 (de) * 1996-09-19 2006-01-15 Novozymes As Wirtszellen und methoden für die produktion von proteinen
WO1999007862A1 (en) 1997-08-05 1999-02-18 Chiron Corporation Novel pichia pastoris gene sequences and methods for their use
AU9231898A (en) * 1997-09-15 1999-04-05 Zymogenetics Inc. Protease-deficient strains of (pichia methanolica)
US6183953B1 (en) 1997-09-15 2001-02-06 Zymogenetics, Inc. Chromosomal mutagenesis in Pichia methanolica
AU752578B2 (en) * 1997-09-15 2002-09-26 Zymogenetics Inc. Chromosomal mutagenesis in pichia methanolica
US6605450B1 (en) 2000-05-25 2003-08-12 Chiron Corporation Iron regulation of the PpSEC10 transcriptional regulatory region of Pichia pastoris and methods of use
US20060034830A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GalGlcNAcMan5GLcNAc2 glycoform
US20060029604A1 (en) * 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
US20060034828A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAcMAN5GLCNAC2 glycoform
US20060257399A1 (en) * 2000-06-28 2006-11-16 Glycofi, Inc. Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform
WO2002101038A1 (fr) 2001-05-29 2002-12-19 Asahi Glass Company, Limited Procede de construction d'un hote et procede de production d'une proteine etrangere
US20060024292A1 (en) * 2001-12-27 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Gal2GlcNAc2Man3GlcNAc2 glycoform
US20060034829A1 (en) * 2001-12-27 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a MAN3GLCNAC2 glycoform
US7972809B2 (en) 2002-04-26 2011-07-05 National Institute Of Advanced Industrial Science & Technology Methylotrophic yeast producing mammalian type sugar chain
US7514253B2 (en) * 2003-05-16 2009-04-07 Glycofi, Inc. URA5 gene and methods for stable genetic integration in yeast
ES2630224T3 (es) 2003-10-22 2017-08-18 Keck Graduate Institute Métodos para sintetizar polipéptidos heteromultiméricos en levadura con el uso de una estrategia de apareamiento haploide
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CA2531616A1 (en) * 2004-12-28 2006-06-28 Kabushiki Kaisha Kobe Seiko Sho (Kobe Steel, Ltd.) High strength thin steel sheet having high hydrogen embrittlement resisting property and high workability
EP1937305A4 (en) * 2005-09-09 2008-10-08 Glycofi Inc IMMUNOGLOBULIN COMPRISING A MAN7GLCNAC2 OR MAN8GLCNAC2 PREDOMINANT GLYCOFORM
ES2539378T3 (es) * 2009-02-26 2015-06-30 Glaxosmithkline Llc Células huésped y procedimientos de uso
EP2494050A4 (en) * 2009-10-30 2013-10-30 Merck Sharp & Dohme FACTOR FOR STIMULATING GRANULOCYTES AND MONOCYTES PRODUCED IN GLYCOMODIFIED PICHIA PASTORIS
WO2011073883A1 (en) 2009-12-14 2011-06-23 Centre Hospitalier Universitaire Vaudois (Chuv) Pichia pastoris deficient in endogenous secreted protease
JP2014502144A (ja) 2010-10-06 2014-01-30 ビーピー・コーポレーション・ノース・アメリカ・インコーポレーテッド バリアントｃｂｈｉポリペプチド
BR112020016258A2 (pt) 2018-02-12 2020-12-15 Lonza Ltd Uma célula hospedeira eucariótica modificada geneticamente projetada para reduzir a produção de proteínas da célula hospedeira, método de produzir uma proteína de interesse usando a célula hospedeira, método para reduzir a contaminação por proteína da célula hospedeira
JP7061234B2 (ja) 2019-01-11 2022-04-27 ロンザ リミテッド 組換え宿主細胞内での炭素源調節タンパク質産生
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
ES2964319T3 (es) 2019-04-01 2024-04-05 Univ Wien Bodenkultur Levadura metilotrófica Mut-
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Also Published As

Publication number Publication date
CA2105064A1 (en) 1992-10-02
US5831053A (en) 1998-11-03
US5691166A (en) 1997-11-25
US6051419A (en) 2000-04-18
ATE220105T1 (de) 2002-07-15
WO1992017595A1 (en) 1992-10-15
EP0578746A1 (en) 1994-01-19
DE69232666T2 (de) 2003-03-20
AU1750592A (en) 1992-11-02
DE69232666D1 (de) 2002-08-08
JP2003159090A (ja) 2003-06-03
EP0578746B1 (en) 2002-07-03
JPH06506117A (ja) 1994-07-14
US5324660A (en) 1994-06-28
US5541112A (en) 1996-07-30
AU661844B2 (en) 1995-08-10

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