DE69232666D1 - Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung - Google Patents

Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung

Info

Publication number
DE69232666D1
DE69232666D1 DE69232666T DE69232666T DE69232666D1 DE 69232666 D1 DE69232666 D1 DE 69232666D1 DE 69232666 T DE69232666 T DE 69232666T DE 69232666 T DE69232666 T DE 69232666T DE 69232666 D1 DE69232666 D1 DE 69232666D1
Authority
DE
Germany
Prior art keywords
pichia
proteolytic activity
genes
genes influencing
strains
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69232666T
Other languages
English (en)
Other versions
DE69232666T2 (de
Inventor
Martin Anthony Gleeson
Bradley Drake Howard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Application granted granted Critical
Publication of DE69232666D1 publication Critical patent/DE69232666D1/de
Publication of DE69232666T2 publication Critical patent/DE69232666T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/938—Pichia

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69232666T 1991-04-01 1992-03-31 Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung Expired - Fee Related DE69232666T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67891691A 1991-04-01 1991-04-01
PCT/US1992/002521 WO1992017595A1 (en) 1991-04-01 1992-03-31 Genes which influence pichia proteolytic activity, and uses therefor

Publications (2)

Publication Number Publication Date
DE69232666D1 true DE69232666D1 (de) 2002-08-08
DE69232666T2 DE69232666T2 (de) 2003-03-20

Family

ID=24724845

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69232666T Expired - Fee Related DE69232666T2 (de) 1991-04-01 1992-03-31 Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung

Country Status (9)

Country Link
US (5) US5324660A (de)
EP (1) EP0578746B1 (de)
JP (2) JPH06506117A (de)
AT (1) ATE220105T1 (de)
AU (1) AU661844B2 (de)
CA (1) CA2105064A1 (de)
DE (1) DE69232666T2 (de)
ES (1) ES2177528T3 (de)
WO (1) WO1992017595A1 (de)

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US5594119A (en) * 1994-09-20 1997-01-14 Novo Nordisk A/S Gene encoding carboxypeptidase of aspergillus niger
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US6153424A (en) * 1995-11-09 2000-11-28 Zymogenetics, Inc. Protease-deficient strains of Pichia methanolica
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CN1120235C (zh) 1996-03-27 2003-09-03 诺沃奇梅兹有限公司 碱性蛋白酶缺陷型丝状真菌
US6110703A (en) * 1996-07-05 2000-08-29 Novo Nordisk A/S Method for the production of polypeptides
ATE313620T1 (de) * 1996-09-19 2006-01-15 Novozymes As Wirtszellen und methoden für die produktion von proteinen
WO1999007862A1 (en) 1997-08-05 1999-02-18 Chiron Corporation Novel pichia pastoris gene sequences and methods for their use
AU9231898A (en) * 1997-09-15 1999-04-05 Zymogenetics Inc. Protease-deficient strains of (pichia methanolica)
US6183953B1 (en) 1997-09-15 2001-02-06 Zymogenetics, Inc. Chromosomal mutagenesis in Pichia methanolica
AU752578B2 (en) * 1997-09-15 2002-09-26 Zymogenetics Inc. Chromosomal mutagenesis in pichia methanolica
US6605450B1 (en) 2000-05-25 2003-08-12 Chiron Corporation Iron regulation of the PpSEC10 transcriptional regulatory region of Pichia pastoris and methods of use
US20060034830A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GalGlcNAcMan5GLcNAc2 glycoform
US20060029604A1 (en) * 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
US20060034828A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAcMAN5GLCNAC2 glycoform
US20060257399A1 (en) * 2000-06-28 2006-11-16 Glycofi, Inc. Immunoglobulins comprising predominantly a Man5GIcNAc2 glycoform
WO2002101038A1 (fr) 2001-05-29 2002-12-19 Asahi Glass Company, Limited Procede de construction d'un hote et procede de production d'une proteine etrangere
US20060024292A1 (en) * 2001-12-27 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Gal2GlcNAc2Man3GlcNAc2 glycoform
US20060034829A1 (en) * 2001-12-27 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a MAN3GLCNAC2 glycoform
US7972809B2 (en) 2002-04-26 2011-07-05 National Institute Of Advanced Industrial Science & Technology Methylotrophic yeast producing mammalian type sugar chain
US7514253B2 (en) * 2003-05-16 2009-04-07 Glycofi, Inc. URA5 gene and methods for stable genetic integration in yeast
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CA2531616A1 (en) * 2004-12-28 2006-06-28 Kabushiki Kaisha Kobe Seiko Sho (Kobe Steel, Ltd.) High strength thin steel sheet having high hydrogen embrittlement resisting property and high workability
EP1937305A4 (de) * 2005-09-09 2008-10-08 Glycofi Inc Immunglobulin, das vorwiegend eine man7glcnac2, man8glcnac2 glycoform enthält
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EP2494050A4 (de) * 2009-10-30 2013-10-30 Merck Sharp & Dohme In glycoengineerter pichia pastoris hergestellter granulozytenkolonienstimulierender faktor
WO2011073883A1 (en) 2009-12-14 2011-06-23 Centre Hospitalier Universitaire Vaudois (Chuv) Pichia pastoris deficient in endogenous secreted protease
JP2014502144A (ja) 2010-10-06 2014-01-30 ビーピー・コーポレーション・ノース・アメリカ・インコーポレーテッド バリアントｃｂｈｉポリペプチド
BR112020016258A2 (pt) 2018-02-12 2020-12-15 Lonza Ltd Uma célula hospedeira eucariótica modificada geneticamente projetada para reduzir a produção de proteínas da célula hospedeira, método de produzir uma proteína de interesse usando a célula hospedeira, método para reduzir a contaminação por proteína da célula hospedeira
JP7061234B2 (ja) 2019-01-11 2022-04-27 ロンザ リミテッド 組換え宿主細胞内での炭素源調節タンパク質産生
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
ES2964319T3 (es) 2019-04-01 2024-04-05 Univ Wien Bodenkultur Levadura metilotrófica Mut-
US20230126246A1 (en) 2020-04-01 2023-04-27 Lonza Ltd Helper factors for expressing proteins in yeast
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Also Published As

Publication number Publication date
CA2105064A1 (en) 1992-10-02
US5831053A (en) 1998-11-03
US5691166A (en) 1997-11-25
US6051419A (en) 2000-04-18
ATE220105T1 (de) 2002-07-15
ES2177528T3 (es) 2002-12-16
WO1992017595A1 (en) 1992-10-15
EP0578746A1 (de) 1994-01-19
DE69232666T2 (de) 2003-03-20
AU1750592A (en) 1992-11-02
JP2003159090A (ja) 2003-06-03
EP0578746B1 (de) 2002-07-03
JPH06506117A (ja) 1994-07-14
US5324660A (en) 1994-06-28
US5541112A (en) 1996-07-30
AU661844B2 (en) 1995-08-10

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