ATE313620T1 - Wirtszellen und methoden für die produktion von proteinen - Google Patents

Wirtszellen und methoden für die produktion von proteinen

Info

Publication number
ATE313620T1
ATE313620T1 AT97939990T AT97939990T ATE313620T1 AT E313620 T1 ATE313620 T1 AT E313620T1 AT 97939990 T AT97939990 T AT 97939990T AT 97939990 T AT97939990 T AT 97939990T AT E313620 T1 ATE313620 T1 AT E313620T1
Authority
AT
Austria
Prior art keywords
proteins
methods
host cell
relates
host cells
Prior art date
Application number
AT97939990T
Other languages
English (en)
Inventor
Jan Lehmbeck
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE313620T1 publication Critical patent/ATE313620T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT97939990T 1996-09-19 1997-09-19 Wirtszellen und methoden für die produktion von proteinen ATE313620T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK102496 1996-09-19
PCT/DK1997/000397 WO1998012300A1 (en) 1996-09-19 1997-09-19 Novel host cells and methods of producing proteins

Publications (1)

Publication Number Publication Date
ATE313620T1 true ATE313620T1 (de) 2006-01-15

Family

ID=8100132

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97939990T ATE313620T1 (de) 1996-09-19 1997-09-19 Wirtszellen und methoden für die produktion von proteinen

Country Status (10)

Country Link
US (1) US6352841B1 (de)
EP (1) EP0956338B1 (de)
JP (1) JP4091119B2 (de)
CN (1) CN1262639C (de)
AT (1) ATE313620T1 (de)
AU (1) AU4200897A (de)
DE (1) DE69734936T2 (de)
DK (1) DK0956338T3 (de)
ES (1) ES2256895T3 (de)
WO (1) WO1998012300A1 (de)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1525798T3 (da) 1997-12-22 2012-03-05 Novozymes As Carbohydrat-oxidase og anvendelse deraf i bagning
US6806062B1 (en) 1998-10-05 2004-10-19 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
WO2000024883A1 (en) 1998-10-26 2000-05-04 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
DK1141371T3 (da) * 1998-12-23 2009-02-02 Novozymes As Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller
ATE469223T1 (de) 2000-03-14 2010-06-15 Novozymes As Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
EP2119773A1 (de) 2000-06-26 2009-11-18 Novozymes A/S Lipolytische Enzyme aus Fusarium und Acremonium Stämmen
CN1452656B (zh) 2000-09-05 2011-06-15 诺维信公司 脂肪氧合酶
US7063962B2 (en) 2001-07-20 2006-06-20 Novozymes A/S DNA sequences for regulating transcription
JP2005536989A (ja) * 2002-05-24 2005-12-08 ユニベルシテ・ラバル 組換えタンパク質の生産用に形質転換された真菌
AU2003298577A1 (en) * 2002-09-10 2004-05-04 Genencor International, Inc. Induction of gene expression using a high concentration sugar mixture
BRPI0409599B1 (pt) 2003-04-28 2014-09-16 Novozymes As Sequência de ácido nucléico, construto de ácido nucléico, vetor de expressão recombinante, micro-organismo recombinante, composição de massa, métodos de produzir uma fosfolipase, de preparar uma massa ou um produto assado feito da massa, processos para reduzir o conteúdo de fósforo em um óleo vegetal e para produzir queijo
EP1682655B1 (de) 2003-10-30 2009-09-02 Novozymes A/S Kohlenhydratbindende module
WO2005083060A1 (en) 2004-03-01 2005-09-09 Kitakyushu Foundation For The Advancement Of Industry, Science And Technology Human cell strains for protein production, provided by selecting strains with high intracellular protein and mutating with carcinogens
GB0405659D0 (en) * 2004-03-12 2004-04-21 Horticulture Res Internat Altered expression in filamentous fungi
DK1831384T3 (en) 2004-12-22 2015-11-23 Novozymes As Polypeptides with glucoamylase activity and polynucleotides encoding them.
JP2008527985A (ja) 2005-01-24 2008-07-31 ディーエスエム アイピー アセッツ ビー．ブイ． 糸状菌細胞において目的の化合物を産生させるための方法
US7696318B2 (en) 2005-07-13 2010-04-13 Novo Nordisk Health Care Ag Host cell protein knock-out cells for production of therapeutic proteins
EP1930428B1 (de) * 2005-08-03 2010-12-01 Asahi Glass Company, Limited Transformierte Hefezellen UND VERFAHREN ZUR HERSTELLUNG VON FREMDPROTEIN
CN101292024B (zh) * 2005-10-17 2012-04-18 诺维信公司 用于表达抗体的真菌突变体的用途
CN101883848A (zh) * 2007-10-01 2010-11-10 诺维信公司 具有蛋白酶活性的多肽和编码该多肽的多核苷酸
CN105586355B (zh) * 2008-09-30 2020-05-22 诺维信股份有限公司 在镶片镰孢的酶缺陷突变体中产生多肽的方法
AU2010275672A1 (en) 2009-07-22 2012-02-02 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
BR122018077148B1 (pt) 2009-12-18 2020-11-03 Novozymes, Inc mutante de uma cepa de trichoderma reesei parental isolada, e, métodos para produzir um polipeptídeo de inetresse e para obter um mutante de uma cepa de trichoderma reesei parental
WO2011158623A1 (ja) * 2010-06-17 2011-12-22 キッコーマン株式会社 麹菌染色体における大領域重複の作製方法
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
DK2800809T3 (en) 2012-01-05 2018-05-22 Glykos Finland Oy PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
JP6081245B2 (ja) * 2012-04-07 2017-02-15 キッコーマン株式会社 麹菌染色体における任意領域の重複転座の作製方法
US9695454B2 (en) 2012-05-23 2017-07-04 Glykos Finland Oy Production of fucosylated glycoproteins
JP2016523552A (ja) 2013-07-10 2016-08-12 ノバルティス アーゲー 多重プロテアーゼ欠損糸状菌細胞及びその使用方法
CN108064266A (zh) 2014-07-21 2018-05-22 格利科斯芬兰公司 在丝状真菌中具有哺乳动物样n-聚糖的糖蛋白的制备
CN119286826A (zh) 2014-12-19 2025-01-10 诺维信公司 包括具有木聚糖酶活性的多肽和具有阿拉伯呋喃糖苷酶活性的多肽的组合物
TW201702380A (zh) * 2015-02-27 2017-01-16 再生元醫藥公司 宿主細胞蛋白質修飾
JP6245299B2 (ja) * 2016-04-27 2017-12-13 バクスアルタ ゲーエムベーハー 組換え安定細胞クローン、その産生およびその使用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5480794A (en) 1987-06-29 1996-01-02 Massachusetts Institute Of Technology And Metabolix, Inc. Overproduction and purification of soluble PHA synthase
GB8727772D0 (en) * 1987-11-27 1987-12-31 Celltech Ltd Host cells
ATE248925T1 (de) * 1988-01-05 2003-09-15 Roche Diagnostics Gmbh Verfahren zur herstellung von proteinen oder proteinhaltigen genprodukten
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
CA2105064A1 (en) * 1991-04-01 1992-10-02 Martin Anthony Gleeson Genes which influence pichia proteolytic activity, and uses therefor
US5674728A (en) * 1993-11-03 1997-10-07 Novartis Corporation Aspergillus niger vacuolar aspartyl protease
US5589364A (en) 1994-07-29 1996-12-31 Magainin Pharmaceuticals Inc. Recombinant production of biologically active peptides and proteins
JP3974171B2 (ja) * 1995-03-20 2007-09-12 ノボザイムス アクティーゼルスカブ 低下したレベルのメタロプロテアーゼを発見する宿主細胞及びその細胞をタンパク質の生産に用いる方法
ATE317005T1 (de) 1996-03-27 2006-02-15 Novozymes As Alkaline-phosphatase-defiziente, filamentöse pilze

Also Published As

Publication number Publication date
ES2256895T3 (es) 2006-07-16
DE69734936T2 (de) 2006-08-24
EP0956338B1 (de) 2005-12-21
JP4091119B2 (ja) 2008-05-28
WO1998012300A1 (en) 1998-03-26
DK0956338T3 (da) 2006-05-08
CN1230986A (zh) 1999-10-06
EP0956338A1 (de) 1999-11-17
AU4200897A (en) 1998-04-14
CN1262639C (zh) 2006-07-05
JP2001500381A (ja) 2001-01-16
US6352841B1 (en) 2002-03-05
DE69734936D1 (de) 2006-01-26

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