ATE256191T1 - Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae - Google Patents

Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae

Info

Publication number
ATE256191T1
ATE256191T1 AT95922443T AT95922443T ATE256191T1 AT E256191 T1 ATE256191 T1 AT E256191T1 AT 95922443 T AT95922443 T AT 95922443T AT 95922443 T AT95922443 T AT 95922443T AT E256191 T1 ATE256191 T1 AT E256191T1
Authority
AT
Austria
Prior art keywords
area gene
pct
aspergillus oryzae
modified
mushroom
Prior art date
Application number
AT95922443T
Other languages
English (en)
Inventor
Tove Christensen
Michael J Hynes
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE256191T1 publication Critical patent/ATE256191T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
AT95922443T 1994-06-17 1995-06-19 Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae ATE256191T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK71794 1994-06-17
PCT/DK1995/000254 WO1995035385A1 (en) 1994-06-17 1995-06-19 A FUNGUS WHEREIN THE areA GENE HAS BEEN MODIFIED AND AN areA GENE FROM ASPERGILLUS ORYZAE

Publications (1)

Publication Number Publication Date
ATE256191T1 true ATE256191T1 (de) 2003-12-15

Family

ID=8096735

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95922443T ATE256191T1 (de) 1994-06-17 1995-06-19 Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae

Country Status (10)

Country Link
US (1) US6025185A (de)
EP (1) EP0770139B1 (de)
JP (1) JPH10501414A (de)
CN (1) CN1253574C (de)
AT (1) ATE256191T1 (de)
AU (1) AU2733895A (de)
DE (1) DE69532283T2 (de)
DK (1) DK0770139T3 (de)
FI (1) FI965031L (de)
WO (1) WO1995035385A1 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0866872A1 (de) * 1995-12-15 1998-09-30 Novo Nordisk A/S Ein pilz, bei dem die gene area. pepc und / oder pepe inaktiviert wurden
ATE373493T1 (de) 1996-07-05 2007-10-15 Novozymes As Alpha-amylase transkriptionsfaktor
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
DE69729085T2 (de) * 1997-07-05 2005-04-21 Nestle Sa Gesteigerte Expression proteolytischer Enzyme in ...
EP1117798B1 (de) * 1998-10-05 2006-12-27 Novozymes A/S Transkriptionsaktivator prtt aus dem pilz aspergillus niger verwendbar in verfahren zur herstellung von polypeptiden
US6806062B1 (en) 1998-10-05 2004-10-19 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
EP1117808B1 (de) * 1998-10-06 2004-12-29 Mark Aaron Emalfarb Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen
CN1197965C (zh) 1998-10-26 2005-04-20 诺维信公司 在丝状真菌细胞内构建和筛选目的dna文库
ATE469223T1 (de) * 2000-03-14 2010-06-15 Novozymes As Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
EP2505651A3 (de) 2006-12-10 2013-01-09 Dyadic International, Inc. Pilzisolate mit reduzierter Proteaseaktivität
CA2736661A1 (en) * 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
DK2438163T3 (en) 2009-06-02 2015-04-20 Novozymes Inc Polypeptides having cellobiohydrolase activity and polynucleotides encoding them
US8143021B2 (en) 2009-07-07 2012-03-27 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CA2775358A1 (en) 2009-09-29 2011-04-07 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
BR112012006978B1 (pt) 2009-09-29 2018-11-06 Novozymes, Inc. célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo tendo atividade de xilanase, para degradar ou converter um material celulósico ou um material contendo xilano, para produzir um produto de fermentação, para fermentar um material celulósico ou um material contendo xilano, construção contendo ácido nucleico, e, vetor de expressão.
CA2775353A1 (en) 2009-09-30 2011-04-07 Novozymes, Inc. Polypeptides derived from thermoascus crustaceu having cellulolytic enhancing activity and polynucleotides encoding same
EP3222716B1 (de) 2009-11-06 2020-08-19 Novozymes, Inc. Zusammensetzungen zur verzuckerung von cellulosematerial
EP2635594B1 (de) 2010-11-04 2017-01-11 Novozymes Inc. Polypeptide mit cellobiohydrolaseaktivität und dafür kodierende polynukleotide
US9139823B2 (en) 2010-11-12 2015-09-22 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
BR112013016830A2 (pt) 2011-02-23 2017-03-01 Novozymes Inc polipeptídeo isolado, polinucleotídeo isolado, método de produzir o polipeptídeo, de produzir um mutante de uma célula parental, de inibir a expressão de um polipeptídeo, de produzir uma proteína, de degradar ou converter um material celulósico, de produzir um produto de fermentação e de fermentar um material celulósico, planta transgênica, parte da planta ou célula de planta transformada com um polinucleotídeo, molécula de rna de fita dupla, composição, e, formulação de caldo completo ou composição de cultura de células
DK2689011T3 (en) 2011-03-25 2018-01-22 Novozymes As PROCEDURE FOR DEGRADATION OR CONVERSION OF CELLULOSE-SUBSTANCING MATERIAL
CN103797126A (zh) 2011-04-29 2014-05-14 诺维信股份有限公司 用于增强纤维素材料的降解或转化的方法
US10308921B2 (en) 2011-10-31 2019-06-04 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
EP3342860A1 (de) 2011-11-22 2018-07-04 Novozymes, Inc. Polypeptide mit beta-xylosidase-aktivität und dafür codierende polynukleotide
EP2785732B1 (de) 2011-12-01 2017-04-12 Novozymes, Inc. Polypeptide mit beta-xylosidase-aktivität und dafür codierende polynukleotide
EP2791330B1 (de) 2011-12-16 2017-07-26 Novozymes, Inc. Polypeptide mit laccaseaktivität und dafür kodierende polynukleotide
WO2013091547A1 (en) 2011-12-19 2013-06-27 Novozymes, Inc. Polypeptides having catalase activity and polynucleotides encoding same
US9567596B2 (en) 2012-01-05 2017-02-14 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
BR112015014624A2 (pt) 2012-12-24 2017-10-10 Novozymes As polipeptídeo isolado, composição, polinucleotídeo isolado, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, e, método para tratamento de têxtil
MX2016000306A (es) 2013-07-10 2016-08-08 Novartis Ag Celulas de hongos filamentosos deficientes en multiples proteasas y métodos de uso de las mismas.
US10619144B2 (en) 2013-08-23 2020-04-14 Novozymes A/S Regulated PepC expression
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190931A (en) * 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US4885249A (en) * 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
EP0206783A3 (de) * 1985-06-20 1988-10-05 The Salk Institute Biotechnology Industrial Associates, Inc. Expression und Sekretion von Polypeptiden aus Saccharomyces cerevisiae
DK122686D0 (da) * 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
ES2204884T3 (es) * 1988-01-05 2004-05-01 Roche Diagnostics Gmbh Procedimiento para la obtencion de proteinas o productos genicos que contienen proteinas.
CA1333777C (en) * 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
ATE220105T1 (de) * 1991-04-01 2002-07-15 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung

Also Published As

Publication number Publication date
CN1150824A (zh) 1997-05-28
DE69532283D1 (de) 2004-01-22
DE69532283T2 (de) 2004-09-23
EP0770139B1 (de) 2003-12-10
DK0770139T3 (da) 2004-04-13
WO1995035385A1 (en) 1995-12-28
FI965031A7 (fi) 1996-12-16
US6025185A (en) 2000-02-15
JPH10501414A (ja) 1998-02-10
FI965031A0 (fi) 1996-12-16
CN1253574C (zh) 2006-04-26
AU2733895A (en) 1996-01-15
EP0770139A1 (de) 1997-05-02
FI965031L (fi) 1996-12-16

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